1100/1200 Series Injector Programming
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1 1100/1200 Series Injector Programming This document is believed to be accurate and up-to-date. However, Agilent Technologies, Inc. cannot assume responsibility for the use of this material. The information contained herein is intended for use by informed individuals who can and must determine its fitness for their purpose. F01047.doc Page 1 of 1
2 1100/1200 Series Injector Programming Injector Program The injector program comprises a series of numbered lines, each of which defines an operation that the injector carries out sequentially. When you activate an injector program, it replaces the standard injection cycle. By default the number of steps an injector program can include is limited. Use the CP variable ExtInjProg to allow for more steps in the injector program. For LC/MSD analyses in which the injector program lasts more than 10 min. Select the [Change] button to confirm changes to the currently-selected line. Select the [Insert] button to insert the contents of the edit line above the currently selected line. Select the [Append] button to add the contents of the edit line to the end of the table. Select the [Cut] button to delete the currently-selected line and place it on the clipboard. Select the [Copy] button to copy the currently selected line to the clipboard. Select the [Paste] button to paste the line on the clipboard at the current position. This document is believed to be accurate and up-to-date. However, Agilent Technologies, Inc. cannot assume responsibility for the use of this material. The information contained herein is intended for use by informed individuals who can and must determine its fitness for their purpose. BCDAEB7C.doc Page 1 of 5
3 You can select the following functions for entry into the injector program: Draw : Enables the injector to draw from a specified Source. Amount Source Speed Offset : Def., the default amount specified in the Setup Injector dialog box Max., the maximum amount defined by the syringe and capillary : Sample specific VialSeatAirSample+ : Def., the default speed specified in the Setup Injector dialog box Max., the maximum draw speed : -2.5 to +35 mm below or above the normal draw position Def., the default draw position specified in the Setup Injector dialog box Eject: Moves to a specified position and ejects a specified amount from the needle. Amount : Def., the default amount specified in the Setup Injector dialog box Max., the maximum amount Destination: Sample specific VialSeatSample+ Speed : Def., the default speed specified in the Setup Injector dialog box Max., the maximum draw speed Offset : -2.5 to +35 mm below or above the normal draw position Def., the default draw position specified in the Setup Injector dialog box Mix : Mixes the drawn volume in the air or seat by moving the injector piston back and forth. Amount Source Speed Repeat : Def., the default amount specified in the Setup Injector dialog box Max., the maximum amount : SeatAir : Def., the default speed specified in the Setup Injector dialog box Max., the maximum draw speed : specify the number of repeat cycles Inject: Completes the injection by moving the needle to the seat, turning the valve to mainpass, and generating a start pulse. BCDAEB7C.doc Page 2 of 5
4 Wait: Autosampler waits for a specified time between 0 and minutes. A time of 0 is equivalent to Off. Note that the Wait time is accurately controlled. Valve Parameter Action: Mainpass : Turns the valve to the mainpass position Main+Start : Turns the valve to the mainpass position and generates a start pulse. Bypass : Turns the valve to the bypass position. Main In : Turns the valve to the mainpass position while the needle is at the Source for a Time between 1 and 99 seconds. The source can be a Sample, a Vial, Seat or Sample+. Needle: Moves the injector needle as specified. Up : Moves the needle to its upper position. Down Into : Moves the needle into a source. The source can be a Sample, a Vial or Sample+. An Offset of -2.5 to +35 mm below or above the normal draw position can also be specified. Wash: Washes the needle in a wash vial for which you can specify a vial number or select DEF to use the vial specified in the Setup Injector dialog box. Specify up to 5 Repeat cycles. Remote: Specifies the remote pulses generated by the autosampler and transmitted on the remote bus. Parameter Action: Ready : Sets the Ready state Not Ready : Sets the Not Ready state StartPulse : Sends a Start pulse StopPulse : Sends a Stop pulse PreparePulse : Sends a Prepare pulse to the detector StartRequest : Sends a Start Request pulse BCDAEB7C.doc Page 3 of 5
5 Wait For: Waits for changes in the remote line. You can specify a time-out range between 0 and minutes. Parameter Action: Ready Start StartRequest Stop : Waits for a Ready state : Waits for a Start pulse : Waits for a Start Request pulse : Waits for a Stop pulse Repeat: Repeats the lines of the injector program between the Repeat and End Repeat functions the specified number of times. Note that no nested repeat statements are allowed. End Repeat: Marks the end of the injector program lines to be repeated. Comment: Enables you to type a free text comment into the injector program. Contact: The Contact command is available only if external contacts are configured. It enables you to set the state of the external contacts. Output: The Output is available only if external contacts are configured and used for binary output. It enables a specified Number to be output on the BCD connector in binary format. Before the injector program is accepted, the syntax is checked for errors. The syntax must be correct for the injector program to be accepted. Move vial: Gets a vial at the FROM position, and places it at the TO position. Parameter Explanation FROM TO Limits: The position, where the vial will be used from vial positions, the ALS supports The position, where the vial will be moved to. 1.-.vial positions, the ALS supports BCDAEB7C.doc Page 4 of 5
6 The following picture is a screen capture of a basic injector program which involves a default volume (volume specified under injection volume-20ul) draw from the sample and a default volume (volume specified under injection volume-20ul) draw from another vial (#1). This total volume of 40ul is then being mixed in the needle seat capillary 6X. One cycle of mixing entails drawing the syringe back a total of 40ul and mixing this volume by moving the syringe forward and back, mixing the 40ul. The final step of the injector program is to inject the sample onto the column. No command for inject is required. BCDAEB7C.doc Page 5 of 5
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