DART SVP. Software Manual For Web Based User Interface And For Apple ipod touch User Interface. Software Version 4.0.x Document #: 7.5.

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DART SVP Software Manual For Web Based User Interface And For Apple ipod touch User Interface Software Version 4.0.x Document #: 7.5.051 Revision: 6 IonSense Inc. 999 Broadway Suite 404 Saugus, MA 01906 2

Table of Contents For Web Based Interface Controlling the DART-SVP from your PC 1 Setting up Network Connections 1 Accessing the DART-SVP in your web browser 1 DART Software Overview 2 Free Run Page 3 Linear Rail Controls 4 DART Controls 5 Status Area: 6 Apple ipod touch User Interface Overview of the Apple ipod touch 17 Turning on the ipod 17 Navigating the Home Screen 18 Accessing the DART Application on the ipod touch 19 Updating the Web Software 19 Troubleshooting 21 Unable to Connect 22 Linear Rail not Connected 25 Dart Source not Connected 26 Methods Page 7 Edit the Method 8 Add the Method 8 Running the Method 9 Pause the Method 10 Edit the Method 11 Settings Page 12 About Screen 13 Grid Voltage 14 X and Y Rail Settings 15 Contact Closure 16 4

Controlling the DART-SVP from your PC The DART-SVP controller runs a web server that you can connect to using an Ethernet cable and view inside a web browser. There is no software to install and it works on any modern operating system. Setting up Network Connections: In order to communicate with the DART-SVP, your computer needs to have a static IP address. Please follow the instructions in the SVP Network installation manual. DART Software Overview The DART software has three main pages for controlling the DART source and automation. Go to the page by clicking on its name or icon. Note: You can navigate the entire user interface using only the keyboard. Press the tab key to cycle between interactive areas. Press enter to activate the selected choice. Accessing the DART-SVP in your web browser: Once your network connections are set up, power on your DART-SVP. The Ethernet connection that it is connected to should become active. Open your PCs web browser. We strongly recommend using the latest version of firefox. Navigate to this address: http://192.168.10.111 You should see a page that looks like the one below: Methods Free Run Automated control to run multiple samples and reproduce results. Direct Control of the DART source and linear rails. Settings Configure advanced values. 1 2

Free Run Page Linear Rail Controls The main page of the DART software is called the Free Run page. From this page the user has the ability to manually control the linear rails and the DART source. Tabs Click X or Y to select the respective rail. X is currently selected. Left Arrow (white) Clicking this button will move the rail to the left. Motion stops when a limit switch is reached. Linear Rail DART Status Area Control the X or Y rail Move to a target position, specified in Settings System State Gas Ion Mode Set Temperature Connections High Voltage Indicator Actual Heater Temperature Right Arrow (black) Stop Button Speed Move To Target The rail cannot be moved to the right because it is at the limit switch. The rail is currently stopped, so the stop button is highlighted. (blue) Select the desired speed from the dropdown. If the rail is moving, the speed will change as soon as a new value is selected. Moves to the set target position. (see Settings) Note: The rail must be homed (moved to the left switch) before this feature is active. 3 4

DART Controls Status Area Connection The web page is communicating with the DART controller. Interlock The DART source is properly connected. System State Run: Voltages on, gas and heater enabled Standby: Gas and heater enabled Off: Voltages, gas* and heater are off High Voltage DART is running and creating ions. Only active in Run mode Gas Selection Available in Standby and Run mode Helium (He) Nitrogen (N2) Heater Temperature The temperature inside the DART source. Ion Mode Positive Negative Heater Temperature Can be set in Standby or Run mode Temperature measures the heater inside the source, not the gas temperature. Note: Gas will continue to flow while the heater is more than 200 C. It shuts off automatically when cool enough. 5 6

Methods Page Edit Method Methods enable you to automate your sampling process for greater reproducibility. Future software updates may bring additional methods and features. Delete Edit Click the -icon to remove the method from the list. Click the method to change its parameters. Done Note Returns to the load screen. Removing all methods restores the defaults. Add Method Load Method Click on the method to load it. Edit Click the method to edit its parameters. Methods can be removed from the list. Add Click the method to edit its parameters and add it. Add Add methods to this list from the set of predefined methods. Duplicate methods will be numbered. Cancel Returns to the load screen. 7 8

Running the Method Pause the Method While the method is running, you can pause at any time and resume from where you left off. This causes the rail to stop moving. No additional method steps will be processed, but the gas will stay on and the heater will continue to go to the temperature that it was set to. Pausing does not stop the timer when the method is waiting for a set period of time. Resuming will go right into the next step if the original timer period has expired. Load Return to the Load Screen Kill Stops all rail motion Turns off the DART Start Begin the method Status updated and timer started. Edit Change method parameters. Available when the method is stopped. 9 10

Edit the Method Settings Page Each method has its own parameters that can be edited. Use the text input boxes to change the values and click the save button to commit the changes and return to the run screen. The software will prevent you from entering invalid data. Pressing the back button will return to the run screen without saving your changes. About Grid Voltage Default Gases X / Y Rail Contact Closure View version information about your system. Change the voltage of the grid for each Ion Mode Controls which gas is selected when entering run and standby modes. Adjust Rail Settings and Target Position Use the contact closure signal with methods. 11 12

About Screen Grid Voltage Range 0 to 530 volts Software Version Firmware Version Hardware Version IP Address MAC Address Web Application Updated by the ipod app or from a.bin file Runs inside the Controller Not user upgradable SVP Controller Model Controller's Web Address Type this number into a web browser to access this software (local only) Address of the Controller's Ethernet port Can be used by a network administrator to change the IP address Note The grid voltage is not typically changed after installation. 13 14

X and Y Rail Settings Contact Closure Settings Motor Location Left or Right Based on looking at the MS instrument. Wait for Signal in Sets DART to wait for signal from Mass Spec Sample From Side the methods start on. Typically the same as the motor location. Target Position Use Current Position Used by the Move To Target button Front edge of rail mount lines up with markings on the rail. Sets the target position to the current location of the rail. 15 16

Overview of the Apple ipod Touch The Apple ipod touch provides a self-contained, intuitive, and revolutionary user interface for the DART-SVP system. Turning on the ipod Navigating the Home Screen Once the ipod has been unlocked, the home screen will be displayed. The DART-SVP uses the built in photo and video capabilities of the ipod are used to store relevant installation and application videos. Configure Wi-Fi Settings Most of the time, the ipod is in a standby state, which it can be awakened from by pressing the button under the screen. It will return to the standby state when not used for 5 minutes, which is a setting that can be changed. Application Videos From the locked position the ipod can be unlocked or activated by simply sliding the arrow on the touch screen to the right. If the ipod does not turn on when the center button is pressed, it may be powered down or out of battery power. To power it on, hold the button on the top of the device for several seconds. An apple logo will appear if it has power. If nothing happens after 10 seconds, connect the ipod to a powered USB port on a PC or on the DART SVP using the supplied cable. The screen will show a battery indicator, but will not be unlockable until it has at least a 10% charge. 17 18

Accessing the DART Application on the ipod touch To open the DART software from the ipod home screen, simply touch the Safari icon. 2. Connect to the upload page: While the DART-SVP controller is running and connected to your PC, navigate to this page: Firmware Versions above 2.2.8 http://192.168.10.111/update Firmware Versions 2.2.8 and below: http://192.168.10.111/mpfsupload Then enter the following IP address into the web browser o 192.168.10.111 3. Upload the.bin file: Click the browse button to find the.bin file on your PC. Click the Upload button after your file has been selected. Software Updates Updating the Web Software 1. Acquire the.bin file: When an update is available, IonSense will provide you with a.bin file. It may be emailed to you or made available online. The file size is around 150 KB, but may become slightly larger as new features are added. 4. Wait 15 seconds: The upload only takes a few seconds to complete. When it is finished, a link to the homepage will be displayed. 5. Confirm the new version: View the About screen on the settings page to verify that the version displayed is the one that you uploaded. If it is not, you should perform a hardrefresh in your browser (typically Ctrl+F5). This will clear cached data and reload the files from the DART-SVP. 19 20

Troubleshooting PC - Unable to Connect ipod - Unable to Connect 1. Look for the Wi-Fi icon at the top of the screen. If the symbol is not present, you need to ensure that your ipod's Wi-Fi radio is on and that the D-Link wireless access point on your DART-SVP is connected and powered on. If the symbol is present, be sure that you are not connected to a different wireless network. Your wireless network's SSID is printed on the back of the D-Link access point. It starts with DART. 2. Check your wireless setting If you get this message in your web browser when trying to access the DART, it means that the controller is not properly connected or your network settings are not correct. Please check the Network Installation Manual. 21 22

3. Tap Wi-Fi to check the settings. 5. Confirm IP Address Settings Wi-Fi Network Confirm Settings Switch should be ON. DART### should be selected with a check mark. If it is not listed, check the D-Link unit. Tap the blue arrow to check the IP Address These are the settings needed to connect to the DART-SVP using the provided D-Link access point. See your network administrator if you are connecting to a private network instead. Note: If you are connecting a second device (iphone, laptop, etc.), each device needs a unique IP Address. The first 3 sets of numbers need to be the same (192.168.10) and the last number can range from 0 to 254, but cannot be 111. 4. Check the D-Link access point. D-Link This unit connects the DART-QS to the ipod wirelessly. Both the Ethernet and power cables must be connected and all three lights should be on. LAN and WAN will blink when data is being transferred. AP Switch For the DART-SVP to work properly, this switch on the back must be set to AP 23 24

Linear Rail Not Connected DART Source Not Connected DART OS Mode DART control still works. Rail controls are hidden. The DART source must be connected to generate ions. The linear rails can still be used without a source attached. Methods Will not work without the linear rail. 25 26

IonSense Inc. 999 Broadway Suite 404 Saugus, MA 01906 27