MICROSCOPE COMPONENTS FOR TRANSMITTED LIGHT APPLICATIONS

Transcription

1 MICROSCOPE COMPONENTS FOR TRANSMITTED LIGHT APPLICATIONS

2 Contents System Diagram System Diagram... CFI 0 Objectives CFI Achromat Series for Brightfield/Phase Contrast (DL)... 4~ CFI Achromat 4x CFI Achromat x / CFI Achromat DL x CFI Achromat LWD 0x / CFI Achromat LWD DL 0x CFI Achromat 40x / CFI Achromat DL 40x CFI Achromat 0x CFI Achromat 0x Oil / CFI Achromat DL 0x Oil CFI Achromat LWD DL 0x F CFI Achromat LWD 40x C / CFI Achromat LWD DL 40x C CFI Achromat Series for Brightfield/Phase Contrast (DL)... ~7 CFI Achromat UW 1x CFI Achromat UW x CFI Achromat 4x CFI Achromat x / CFI Achromat DL x CFI Achromat 0x / CFI Achromat DL 0x CFI Achromat 40x / CFI Achromat DL 40x CFI Achromat 40x NCG CFI Achromat 0x Oil CFI Achromat 0x Oil / CFI Achromat DL 0x Oil CFI Achromat 0x NCG CFI Series for Brightfield/Phase Contrast (DLL)... 8~9 CFI 4x CFI x / CFI DLL x CFI 0x / CFI DLL 0x CFI 40x / CFI DLL 40x CFI 40x Oil CFI 0x C CFI 0x Oil / CFI DLL 0x Oil CFI 0x Oil with iris diaphragm CFI Series for Phase Contrast (DL)... 9 CFI DL 4x CFI DL x CFI ELWD Series for Brightfield/Phase Contrast (DM/DLL)... CFI ELWD 0x C / CFI ELWD DM 0x C CFI ELWD 40x C / CFI ELWD DM 40x C CFI ELWD 0x C / CFI ELWD DLL 0x C Multi-Immersion Objective for Brightfield... CFI 0x MI CFI chromat Series for Brightfield/Phase Contrast (DM)... 11~1 CFI chromat x CFI chromat 4x CFI chromat x CFI chromat 0x / CFI chromat DM 0x CFI chromat 40x C / CFI chromat DM 40x C CFI chromat 40x Oil / CFI chromat DM 40x Oil CFI chromat 0x C / CFI chromat DM 0x C CFI chromat 0x Oil / CFI chromat DM 0x Oil CFI chromat 0x WI CFI chromat 0x Oil / CFI chromat DM 0x Oil CFI chromat 0x NCG Oil CFI S Fluor Series... 1 CFI S Fluor x CFI S Fluor 0x CFI S Fluor 40x C CFI S Fluor 40x Oil CFI S Fluor 0x Oil Water Dipping Series CFI Fluor x W CFI Fluor 40x W CFI Fluor 0x W CFI HMC Series for Hoffmann Modulation Contrast... 1 CFI HMC x CFI HMC 0x F CFI HMC LWD 40x C nd Objective Lens Unit... 1 Markings on CFI0 Objectives Condensers... C-C Abbe Condenser, Dry Type C-C Achromat Condenser Achromat Swing-out Condenser C-C Achromat /Aplanat Condenser LWD Achromat Condenser C-C Low Power Condenser Dark Field Condenser, Oil Dark Field Condenser, Dry C-CEL Expander Lens (for darkfield) Illumination Systems... Lamphouse HMX-B Lamphouse HMX-4B, built-in back mirror Lamphouse HMX Mercury Lamp Socket S 0W for HMX-B Halogen Lamp Socket 0W/HMX C-FC Epi-Fl Collector Lens Quartz Epi-Fl Collector Lens Collector Lens/QH0W C-LA HMX Lamphouse Adapter HMX Connecting Adapter SB-X1 (Bayonet to Screw) HMX Connecting Adapter SB-X (Screw to Bayonet) Eyepiece Tubes... Y-TB Binocular Tube Y-TE Ergonomic Binocular Tube Y-TF Trinocular Tube F UW Y-TT Trinocular Tube T UW Intermediate Tubes... Y-FL Epi-Fl Attachment Y-IDP Double Port Eyepieces... CFI x with diopter adjustment CFI x M Photomask with diopter adjustment CFI 1.x with diopter adjustment CFI 1x with diopter adjustment 18~19 Nosepiece... V-NA Motorized Sextuple Nosepiece V-ND MG-DIC Intelligent Sextuple Nosepiece C-N Sextuple Nosepiece C-ND Sextuple DIC Nosepiece CCTV Camera Adapters... ~ V-T Photo Adapter Y-T TV Tube for C-Mount Adapter A C-Mount TV Adapter A Relay Lens 1x I C-Mount TV Adapter ENG-Mount TV Adapter Zoom Lens C-Mount Zooming Adapter ENG-Mount Zooming Adapter C-Mount TV Adapter 0.4x C-Mount TV Adapter 0.x ENG-Mount TV Adapter 0.4x (for Trinocular Tube F, T ) ENG-Mount TV Adapter 0.x (for Trinocular Tube F, T ) C-Mount TV Adapter VM.x C-Mount TV Adapter VM 4x Mercury Lamp Socket S 0W Halogen Lamp Socket 0W/HMX C-C Abbe Condenser (Dry) ENG-Mount TV Adapter ENG-Mount TV Adapter*1* 0.4X, 0.X ENG-Mount CCTV Camera Y-T TV Tube for C-Mount Adapter A Lamphouse HMX-B Lamphouse HMX-4B with Built-in Back Mirror Lamphouse HMX ENG- Mount Zooming Adapter Relay Lens 1X I V-T Photo Adapter C-C Achromat Condenser C-Mount Zooming Adapter Zoom Lens Illumination Systems C-LA HMX Lamphouse Adapter HMX Connecting Adapter SB-X1 (Bayonet to Screw Mount) HMX Connecting Adapter SB-X (Screw to Bayonet Mount) C-FC Epi-Fl Collector Lens Quartz Epi-Fl Collector Lens Collector Lens/QH 0W Achromat Swing-out Condenser C-Mount TV Adapter Relay Lens 1X I Y-TT Trinocular Tube "T" UW Y-TF Trinocular Tube "F" UW Y-TB Binocular Tube Y-TE Ergonomic Binocular Tube C-C Achromat/Aplanat Condenser C-Mount CCTV Camera C-Mount TV Adapter 0.4X, 0.X Condensers C-Mount TV Adapter A LWD Achromat Condenser C-Mount TV Adapter VM.X CFI X with Diopter Adjustment Eyepiece Tubes V-NA Motorized Sextuple Nosepiece C-Mount TV Adapter A C-Mount TV Adapter VM4X CFI XM Photomask with Diopter Adjustment Y-FL Epi-Fl Attachment V-ND MG-DIC Intelligent Sextuple Nosepiece C-C Low Power Condenser C-Mount TV Adapter A CFI 1.X with Diopter Adjustment Y-IDP Double Port Intermediate Tubes CCTV Camera Adapters *1: Use the dedicated 0.4X adapter for the double port sub-port. The 0.X adapter cannot be used with the double port sub-port. *: ENG-mount CCTV adapter 0.4x is a 8-bayonet mount type; other ENG-mount CCTV adapters are 48-bayonet mount types. C-N Sextuple Nosepiece CFI0 Objectives Darkfield Condenser (Oil) C-CEL Expander Lens Eyepieces CFI 1X with Diopter Adjustment Intermediate Tubes Nosepieces C-ND DIC Nosepiece Darkfield Condenser (Dry)

3 CFI Achromat Series for Brightfield/Phase Contrast (DL) Correction for chromatic aberration in these objectives have been dramatically improved and are at the same level as the CFI Achromat Series. These objectives were also corrected for spherical aberration and coma and image flatness across the mm field of view has been drastically improved. The result: truly exceptional quality for this class of objectives. The DL phase contrast objectives in this series have more contrast than the DLL objectives for enhanced contrast with unstained specimens. ø ø ø. ø D 0X/0.4 Ph1 DL / 1. WD C1 4 X/0. /- WD 0 ø.. X/0. /- WD.1 ø ø1 ø 4.1 0X/0.40 / 0.17 WD.8 ø14. ø 4. D 40X/0. WD.1 / CFI Achromat 4x CFI Achromat x CFI Achromat DL x CFI Achromat LWD 0x CFI Achromat LWD DL 0x CFI Achromat LWD DL 0x F CFI Achromat LWD 40x C CFI Achromat LWD DL 40x C X/0. WD 0. / 0.17 ø7 ø1 ø CFI Achromat 40x CFI Achromat DL 40x X/0.80 / 0.17 WD 0. ø7 ø1 ø CFI Achromat 0x X / 1. oil / 0.17 WD 0.18 ø ø1 ø1. ø CFI Achromat 0x Oil CFI Achromat DL 0x Oil Objectives (magnification) N.A. W.D. (mm) Focal length (mm) Physical depth of focus (µm) Weight (g) Remarks Phase ring CFI Achromat 4x CFI Achromat x CFI Achromat DL x ph1 CFI Achromat LWD 0x CFI Achromat LWD DL 0x ph1 CFI Achromat 40x Spring-loaded CFI Achromat DL 40x Spring-loaded ph CFI Achromat 0x Spring-loaded CFI Achromat 0x Oil Spring-loaded CFI Achromat DL 0x Oil Spring-loaded ph CFI Achromat LWD DL 0x F Cover glass thickness 1.mm ph1 CFI Achromat LWD 40x C Cover glass correction 0.mm CFI Achromat LWD DL 40x C Cover glass correction 0.mm ph 4

4 CFI Achromat Series for Brightfield/Phase Contrast (DL) These objectives provide incredible image flatness over the entire mm field of view, with chromatic aberration corrected throughout the entire visible spectrum. These objectives are suitable not only for routine laboratory work but for photomicrography. The DL phase contrast objectives in this series have more contrast than the DLL objectives for enhanced contrast with unstained specimens..7 4 UW 1X/0.04 /- WD..4 UW X/0.0 /- WD X/0. /- WD X/0. N / 0 WD 0.48 ø7. ø14 ø CFI Achromat 40x NCG X/0.90 oil /- WD 0.40 ø. ø14 ø18.8 (NCG) CFI Achromat 0x Oil.4 7 ø1 CFI Achromat UW 1x X/0. /- WD. ø1 ø19 ø CFI Achromat x CFI Achromat DL x CFI Achromat UW x 0 X/0.40 / 0.17 WD 1. ø7 ø1 ø CFI Achromat 0x CFI Achromat DL 0x ø CFI Achromat 4x 40 X/0. / 0.17 ø ø1 ø WD0.7 CFI Achromat 40x CFI Achromat DL 40x X /1. / 0.17 WD 0.17 ø. ø1 ø1.8 ø CFI Achromat 0x Oil CFI Achromat DL 0x Oil X/0.90 N / 0 WD 0. ø. ø14 ø CFI Achromat 0x NCG Objectives (magnification) N.A. W.D. (mm) Focal length (mm) Physical depth of focus (µm) Weight (g) Remarks Phase ring CFI Achromat UW 1x CFI Achromat UW x CFI Achromat 4x CFI Achromat x CFI Achromat DL x ph1 CFI Achromat 0x CFI Achromat DL 0x ph1 CFI Achromat 40x Spring-loaded CFI Achromat DL 40x Spring-loaded ph CFI Achromat 40x NCG Spring-loaded No cover grass CFI Achromat 0x Oil Spring-loaded CFI Achromat 0x Oil Spring-loaded CFI Achromat DL 0x Oil Spring-loaded ph CFI Achromat 0x NCG Spring-loaded No cover grass 7

5 CFI Series for Brightfield/Phase Contrast (DLL) Featuring an extra-high transmission rate, especially in the ultraviolet wavelength, and flatness of field comparable to the CFI Achromat series, these objectives are designed for fluorescence observation and photomicrography. Because of this improvement in quality, these objectives can function as multipurpose objectives for brightfield, fluorescence, polarizing, and DIC observations. The DLL (low contrast) phase objectives for this series can also be used as universal objectives for phase contrast, fluorescence, DIC, and brightfield techniques X / 0.1 / - WD 17.1 CFI 4x. 4.9 X / 0.0 DIC L /0.17 WD 1.0 ø18 CFI x CFI DLL x X / 0.8 DIC M / WD ø18 ø X/1.0 oil DIC H /0.17 WD 0.0 ø.8 ø X/0.-1. oil /0.17 WD ø. ø19.8 ø. C. 8. CFI 0x C CFI 0x Oil CFI DLL 0x Oil CFI 0x Oil with iris diaphragm. 4 0X / 0.0 DIC M /0.17 WD X / 0.7 DIC M /0.17 WD X / 0.1 PhL DL /1. WD X / 1.0 oil DIC H /0.17 WD 0..8 CFI Series for Phase Contrast (DL) These objectives have more contrast than the DLL objectives for enhanced contrast with tissue culture specimens. These objectives are designed for use on inverted microscopes and therefore their markings are upside-down for easy reading ø1 CFI 0x CFI DLL 0x ø CFI 40x CFI DLL 40x 4.7 ø ø.8 CFI 40x Oil X / 0.0 Ph1 DL /1. WD Objectives (magnification) N.A. W.D. (mm) Focal length (mm) Physical depth of focus (µm) Weight (g) Remarks Phase ring CFI 4x CFI x CFI DLL x ph1 CFI 0x CFI DLL 0x ph1 CFI 40x Spring-loaded CFI DLL 40x Spring-loaded ph CFI 40x Oil Spring-loaded Stopper CFI 0x C Spring-loaded Cover glass correction mm CFI 0x Oil Spring-loaded Stopper CFI DLL 0x Oil Spring-loaded Stopper ph CFI 0x Oil with iris diaphragm Spring-loaded With iris diaphragm CFI DL 4x CFI DL x Objectives (magnification) N.A. W.D. (mm) Focal length (mm) Physical depth of focus (µm) Weight (g) Remarks Phase ring CFI DL 4x Cover glass thickness 1.mm phl CFI DL x Cover glass thickness 1.mm ph1 8 9

6 CFI ELWD Series for Brightfield/Phase Contrast (DM/DLL) Offering superb flatness of field, high UV transmission rates, and high Numerical Apertures with extra long working distances, these objectives are well suited for fluorescence observations, especially with inverted microscopes. Because of their superior optical design, CFI ELWD DM objectives can be used universally for all other observation techniques, including brightfield, fluorescence, phase contrast, and Nomarski DIC. The DM phase contrast objectives have higher contrast to enhance the image of very thin, highly detailed specimens with low phase gradients. The DLL objectives have less contrast for thicker, more contrasty specimens. 1. Standard thickness..7 ø. ø1.4.. ø1.. ø. ø CFI chromat Series for Brightfield/Phase Contrast (DM) These objectives feature longer working distances with high Numerical Apertures and is designed to correct all optical aberrations throughout the visible spectrum from violet to red from center to edges across the entire mm field of view. Superior image flatness and color reproduction, plus resolving power at the theoretical limit of today's optical technology are also featured. The DM phase contrast chromats have higher contrast to enhance images of very thin, highly detailed specimens with low phase gradients. LWD 0X/0.4 / 0- WD LWD 40X/0.0 /0- WD WD 0X/ WD X / 0.1 / - WD X / 0. / - WD X / 0.4 DIC L /0.17 WD 4.0 CFI ELWD 0x C CFI ELWD DM 0x C CFI ELWD 40x C CFI ELWD DM 40x C CFI ELWD 0x C CFI ELWD DLL 0x C ø19 WD=8. 0 ø18 WD=1.7 ø1 WD=4.0 Objectives (magnification) N.A. W.D. (mm) Focal length (mm) Physical depth of focus (µm) Weight (g) Remarks Phase ring CFI ELWD 0x C Cover glass correction 0 mm CFI ELWD DM 0x C Cover glass correction 0 mm ph1 CFI ELWD 40x C Cover glass correction 0 mm CFI ELWD DM 40x C Cover glass correction 0 mm ph CFI ELWD 0x C Cover glass correction 0. 1.mm CFI ELWD DLL 0x C Cover glass correction 0. 1.mm ph CFI chromat x CFI chromat 4x CFI chromat x Multi-Immersion Objective for Brightfield This multi-purpose immersion objective has a higher numerical aperture and superior contrast. The use of immersion media helps eliminate surface reflections and provides an image with higher contrast. It is an excellent objective for fluorescence, brightfield, and DIC observation techniques. This unique objective has a correction collar that allows it to be used with immersion oil, glycerin, and water (with and without a cover glass). Objective N.A. W.D. Focal length Physical depth Weight Remarks (magnification) (mm) (mm) of focus (µm) (g) CFI 0x MI Multi-immersion Oil-glycerine-water Oil G ø.. 0X/0.7 Mlmm / - WD W 0 CFI 0x MI X / 0.7 DIC M /0.17 WD 1.0 ø1. ø ø WD=1.0 CFI chromat 0x CFI chromat DM 0x X / 0.9 DIC M / WD ø7 ø18 ø WD=0.14 CFI chromat 40x C CFI chromat DM 40x C ø 40X / 1.0 oil DIC H /0.17 WD 0.1 ø18.8 ø.8 WD=0.1 (oil) CFI chromat 40x Oil CFI chromat DM 40x Oil 11

7 X/0.9 DIC M / WD ø7 ø18 ø WD=0.1 CFI chromat 0x C CFI chromat DM 0x C CFI chromat Series for Brightfield/Phase Contrast (DM) X /1.40 oil DIC H / 0.17 WD 0.1 ø7. ø ø.8 WD=0.1 (oil) CFI chromat 0x Oil CFI chromat DM 0x Oil ø. ø. 0X /1.0 WI / WD ø7..4 CFI chromat 0x WI CFI S Fluor Series These objectives ensure a high transmission rate of ultraviolet wavelengths down to 40 nm for fluorochromes like indo-1, fura-, and fluor-. Also, these objectives have improved signal-to-noise ratios (S/N) for short wavelengths and have high N.A., making the fluorescence images they produce significantly sharper and brighter S Fluor X/ 0.0 / 0.17 WD 0.1 ø19 ø S Fluor 0 X/ 0.7 / 0.17 WD 1.0 ø9 ø ø Fluor 40 X/ 0.9 / WD ø7. ø18 ø CFI S Fluor x CFI S Fluor 0x CFI S Fluor 40x C X /1.40 oil DIC H / 0.17 WD X /1.40 oiln / 0 WD C ø.8 ø1 WD= C 0 ø.8 ø1 WD= S Fluor 40 X/ 1.0 Oil / 0.17 WD S Fluor 0 X /0.-1. oil / 0.17 WD CFI chromat 0x Oil CFI chromat DM 0x Oil CFI chromat 0x NCG Oil. 9.8 Objectives (magnification) N.A. W.D. (mm) Focal length (mm) Physical depth of focus (µm) Weight (g) Remarks Phase ring CFI chromat x CFI chromat 4x CFI chromat x CFI chromat 0x Spring-loaded CFI chromat DM 0x Spring-loaded ph CFI chromat 40x C Spring-loaded Cover glass correction mm CFI chromat DM 40x C Spring-loaded Cover glass correction mm ph CFI chromat 40x Oil Spring-loaded Stopper CFI chromat DM 40x Oil Spring-loaded Stopper ph CFI chromat 0x C Spring-loaded Cover glass correction mm CFI chromat DM 0x C Spring-loaded Cover glass correction mm ph CFI chromat 0x Oil Spring-loaded Stopper CFI chromat DM 0x Oil Spring-loaded Stopper ph CFI chromat 0x WI Spring-loaded Cover glass correction mm Water immersion CFI chromat 0x Oil Spring-loaded Stopper CFI chromat DM 0x Oil Spring-loaded Stopper ph CFI chromat 0x NCG Oil Spring-loaded Stopper No cover glass 4.. ø.8 CFI S Fluor 40x Oil Objectives (magnification) N.A. W.D. (mm) Focal length (mm) Physical depth of focus (µm) Weight (g) Remarks CFI S Fluor x Spring-loaded CFI S Fluor 0x Spring-loaded CFI S Fluor 40x C Spring-loaded Cover glass correction mm CFI S Fluor 40x Oil Spring-loaded CFI S Fluor 0x Oil Spring-loaded.9 ø ø4. CFI S Fluor 0x Oil 1 1

8 Water Dipping Series CFI HMC Series for Hoffmann Modulation Contrast These objectives (no cover glass) are designed with a narrow top and a mm working distance to allow microelectrodes to be inserted into the specimen at 4. They use ceramic and glass nose cones to isolate them thermally and electronically. Since they are FLUOR lenses, they are designed for fluorescence and have very high transmission in the UV and IR wavelengths with a high signal-to-noise ratio for superior images Fluor X / 0.0 W DIC L / 0 WD.0 ø. ø. ø4 ø CFI Fluor x W 48 (Water dipping) Objectives (magnification) N.A. W.D. (mm) Focal length (mm) Physical depth of focus (µm) Weight (g) Remarks CFI Fluor x W Water dipping CFI Fluor 40x W Water dipping CFI Fluor 0x W Water dipping Fluor 40X / 0.80 W DIC M / 0 WD.0 ø4 ø11. ø19 ø ø. CFI Fluor 40x W (Water dipping) Fluor 0X / 1.00 W DIC H / 0 WD.0 ø19. ø ø. ø. CFI Fluor 0x W.7 (Water dipping) 0. These objectives have been completely redesigned by Nikon, allowing the contrast direction to be changed using a modulator inside the objective. That direction, once set, is maintained over the entire magnification range from X to 40X. Note: Hoffman Modulation Contrast and HMC are registered trademarks of Modulation Optics Inc. ø ø1. ø X / 0. / 1. WD. MC1 HMC.8 CFI HMC x nd Objective Lens Unit D 0X / 0.4 / 1. WD.0 MC HMC This unit is attached to infinity objectives when neither a binocular or trinocular eyepiece tube is used. Infinity Tube Lens Unit for CFI objectives (chromatic aberrationfree infinity corrected objectives). Focusing distance: 00mm (7.87 in.) To obtain the optimal objective performance, keep the distance between the tube lens and the objective s shoulder within 0 00 mm ( in.). ø ø1. ø1.8 CFI HMC 0x F Objectives (magnification) N.A. W.D. (mm) Focal length (mm) Physical depth of focus (µm) Weight (g) Remarks CFI HMC x Cover glass thickness 1.mm CFI HMC 0x F Cover glass thickness 1.mm CFI HMC LWD 40x C Cover glass correction0 mm M80. øf ø. ø1 WD 40X / 0. /0- WD MC HMC Approx. 70~ CFI HMC LWD 40x C Image plane Objective's shoulder 0X / 1.0 oil DIC H / 0.17 WD nd Objective Lens Unit 1

9 Markings on CFI0 Objectives Condensers. 8 1 ø (1) Magnification () Numerical Aperture () Application Markings (1) Color Code (4) Correction Ring R40 ø ø4 ø9 R40 ø ø Achr 0.90 JAPAN ø (7) Immersion Oil cleaning () Working Distance () Cover Glass 8 70 Nikon offers a wide variety of CFI objectives. To assist the user they are clearly marked with information on the objective barrel such as: which DIC module or Phase Ring to use. (1) Magnification and Color Code A color coded ring on the barrel identifies the magnification of the objective: Mag. 1X X 4X X 0X 40X 0X 0X 0X Color code Black Gray Red Yellow Green Light Blue Light Blue Cobalt Blue White () Numerical Aperture (N.A.) N.A. is the most important factor in defining the performance characteristics of an objective. N. A.= n sin n: the refractive index of the media at d-line (87nm) For dry objective n=1.000 (air) For oil objective n=1.1 (oil) For water objective n= 1. (water) :Half angle of incident rays to the top lens of the objective The higher N.A., the higher resolving power. When the resolving power is defined as the power to recognize the two points, R= or n = 1.1 N.A. R= N.A. Obj. +N.A. Cond. The higher N.A. the brighter image we take. Brightness: B N.A. Total Magnification The higher N.A., the shallower the depth of focus (DOF). DOF = N.A. Oil n = () Working Distance Working distance (W.D.) defines the distance between the top lens of the objective and the surface of the cover glass. CFI0 objectives can offer longer working distance with high numerical aperture. Dry Objective lens Cover Glass Slide Glass (4) Correction Ring Dry objectives with high Numerical Aperture are susceptible to spherical and other aberrations which can impair resolution and contrast when used with a cover glass whose thickness differs from the specified value. A 1 1/ cover glass (0.17mm thick) should be used as standard, however not all 11/ cover glasses are exactly 0.17mm and many specimens have media between them and the cover glass. The correction ring is used to adjust for these subtle differences to ensure the optimum objective performance. How to use the correction ring Position the ring at The thickness of the standard cover glass is 0.17mm. Focus the lens on a small artifact in the specimen. Rotate the ring very slightly and focus the lens again to check if the image has improved or degraded. Repeat the above step to determine if the image is improving or degrading in the direction you are turning the ring. If the image has degraded, follow the same procedure in the opposite direction to find the position offering optimum resolving power and contrast. () Cover Glass For optimum performance, the thickness of the cover glass should be 0.17mm. For example, at N.A.=0.9, a 0.01mm difference in thickness reduces image formation by 4% from the ideal image. N.A Difference in cover glass thickness 0.01mm 0.0mm 0% % () Application Markings DIC: for Differential Interference Contrast DM: Phase contrast, Dark contrast middle type DL: Phase contrast, Dark contrast light type DLL: Phase contrast, Lower contrast type (7) Immersion Oil cleaning After using immersion oil, gently blot the lens dry with lens tissue. Then slightly moisten a piece of lens tissue with petroleum benzene (Naphtha) and clean off all traces of the oil from the immersion objective. Cleaning is essential for water immersion objectives as well; after use, wipe the water off the top lens. Type N.A. Object distance (mm) Magnification C-C Abbe Condenser, Dry Type x C-C Achromat Condenser x Achromat Swing-out Condenser 0.9/ x C-C Achromat /Aplanat Condenser x LWD Achromat Condenser x C-C Low Power Condenser x Dark Field Condenser, Oil x Dark Field Condenser, Dry x 1 17 ø C-C Abbe Condenser, Dry Type 1.0 (Slidglass thickness) 8. 1 (Slidglass thickness) 0. (Oil) ø4 Achr-ADl N.A=1.4 JAPAN ø C-C Achromat /Aplanat Condenser (Oil) 70 0 ø9. Dark Field Condenser Oil JAPAN ø Dark Field Condenser, Oil ø C-C Achromat Condenser (Slidglass thickness) 1. ø9 ø4. ø.8 ø 1 4. LWD Achromat Condenser ø. Dark Field Condenser Dry JAPAN ø Dark Field Condenser, Dry ø0 R40 Achromat Swing-out Condenser R40 ø ø C-C Low Power Condenser C-CEL C-CEL Expander Lens (for darkfield)

10 Illumination Systems Eyepiece Tubes (Center of lamp) ø7 41. ø7 ø1 ø71 M00.7 ø71 ø1 ø 4 1 (Center of lamp) 4 1 (Center of lamp) Lamphouse HMX-B Lamphouse HMX-4B, built-in back mirror (Datum clamp face) (Datum clamp face) C-LA HMX Lamphouse Adapter (Datum clamp face) (Datum clamp face) HMX Connecting Adapter SB-X1 (Bayonet to Screw) M00.7-H Sectional View HMX Connecting Adapter SB-X (Screw to Bayonet) (Min. distance) 4 (Tilting section) Approx R R40 Y-TB Binocular Tube 8 (Min. angle) ø Y-TE Ergonomic Binocular Tube 10 ø Lamphouse HMX Mercury Lamp Socket S 0W for HMX-B ø0 ø 1. ø (Center of pin) (Center of filament) ø9 1. Halogen Lamp Socket 0W/HMX ø4 1.1 ø C-FC Epi-Fl Collector Lens ø4 1 ø Quartz Epi-Fl Collector Lens ø4 Collector Lens/QH0W 7. Y-TF Trinocular Tube F UW Y-TT Trinocular Tube T UW Y-TB Binocular Tube F.O.V. mm Y-TE Ergonomic Binocular Tube F.O.V. mm with tilting angle of 8 to and telescopic mechanism of ±1mm movement Y-TF Trinocular Tube F UW Two-way: 0 : 0 or 0 : 0 (observation : photo) F.O.V. mm or mm with eyepiece reticule orientation adjustment mechanism Y-TT Trinocular Tube T UW Three-way: 0 : 0, 0 : 80, or 0 : 0 (observation : photo) F.O.V. mm or mm with eyepiece reticule orientation adjustment mechanism 19

11 Intermediate Tubes Nosepieces.. (Top View) V-ND JAPAN V-N JAPAN (Right-side View) ø ø1 V-NA Motorized Sextuple Nosepiece ø11 V-ND MG-DIC Intelligent Sextuple Nosepiece ø11 Y-FL Epi-Fl Attachment Y-IDP Double Port Eyepieces C-ND DIC JAPAN CFI X /.8 CFI X / M CFI x with diopter adjustment CFI x M Photomask with diopter adjustment CFI 1.X / 1 ø.8 CFI 1X / 14. ø ø ø11 C-N Sextuple Nosepiece C-ND Sextuple DIC Nosepiece CFI 1.x with diopter adjustment CFI 1x with diopter adjustment Type (magnification) F.O.V. Remarks CFI x with diopter adjustment mm CFI x M Photomask with diopter adjustment mm With photomask reticule CFI 1.x with diopter adjustment 1mm CFI 1x with diopter adjustment 14.mm 0 1

12 CCTV Camera Adapters Focal point Focal point 7 7 ø ø1 9. ø1 7. C-mount screw 41. TV camera imaging plane ø0 ø C-Mount TV Adapter A ø.1 ø C-Mount TV Adapter 0.4x ø.1 ø C-Mount TV Adapter 0.x Focal point ø ø9 Focal point V-T Photo Adapter Y-T TV Tube for C-Mount Adapter A Relay Lens 1x I. ø4 7 Image plane on eyepiece tube side ø1 ø C-Mount TV Adapter ø47 ø ø ENG-Mount TV Adapter ø.1 ø ENG-Mount TV Adapter 0.4x (for Trinocular Tube F, T ) ø.1 ø ENG-Mount TV Adapter 0.x (for Trinocular Tube F, T ) Focal point JAPAN ø44 ø4 C-mount screw ø44 ø Focal point ø ø ø.1 C-Mount Zooming Adapter ENG-Mount Zooming Adapter C-Mount TV Adapter VM.x Zoom Lens C-Mount TV Adapter VM 4x

13 Specifications and equipment are subject to change without any notice or obligation on the part of the manufacturer. January NIKON CORPORATION ISO 9001 NIKON CORPORATION Instruments Company Accredited by the Dutch Council for Accreditation ISO NIKON CORPORATION Yokohama t NIKON INSTECH CO., LTD. Parale Mitsui Bldg., 8, Higashida-cho, Kawasaki-ku, Kawasaki, Kanagawa -000, Japan phone: fax: NIKON SINGAPORE PTE LTD SINGAPORE phone: fax: +-98 NIKON MALAYSIA SDN. BHD. MALAYSIA phone: fax: NIKON INSTRUMENTS EUROPE B.V. P.O. Box, 1170 AE Badhoevedorp, The Netherlands phone: fax: NIKON FRANCE S.A. FRANCE phone: fax: NIKON GMBH GERMANY phone: fax: NIKON INSTRUMENTS S.p.A. ITALY phone: fax: NIKON AG SWITZERLAND phone: fax: NIKON UK LTD. UNITED KINGDOM phone: fax: NIKON INSTRUMENTS INC. 100 Walt Whitman Road, Melville, N.Y , U.S.A. phone: ; NIKON (within the U.S.A.only) fax: NIKON CANADA INC. CANADA phone: fax: Printed in Japan (001-01)T Code No. CE-MUZH- This brochure is printed on recycled paper made from 40% used material. En