IFC Controller WX USER GUIDE

Transcription

1 PN D1 IFC Controller WX USER GUIDE

2 For Research Use Only. Not for use in diagnostic procedures. Information in this publication is subject to change without notice. It is Fluidigm policy to improve products as new techniques and components become available. Therefore, Fluidigm reserves the right to change specifications at any time. Every effort has been made to avoid errors in the text, diagrams, illustrations, figures, and screen captures. However, Fluidigm assumes no responsibility for any errors or omissions. In no event shall Fluidigm be liable for any damages in connection with or arising from the use of this publication. Patent and Limited License Information Fluidigm products are covered by issued and pending patents in the United States and other countries. Patent and limited license information is available at fluidigm.com/legalnotices Limited Use License to Perform Pre-Amplification with Fluidigm IFCs The purchase of IFCs from Fluidigm Corporation conveys to the purchaser the limited, non-transferable right to perform pre-amplification methods under license from Life Technologies Corporation for use with the purchased amount of this product and Fluidigm instruments. No right to resell this product and no other rights (such as real-time PCR methods, apparatus, reagents or software to perform digital PCR methods) are conveyed by Life Technologies Corporation expressly, by implication, or by estoppel. For information on obtaining additional rights, please contact or Out Licensing, Life Technologies, 5791 Van Allen Way, Carlsbad, California For information about the scope of the above-identified Fields, please contact Limited Digital PCR License A license to use Life Technologies Corporation's patented digital PCR method in all fields other than in the Sequencing Field, the Mass Spectrometry Field and the Prenatal Field in workflows involving a Fluidigm Digital Array IFC can be obtained by (i) with purchase of a Fluidigm Digital Array IFC from Fluidigm Corporation or (ii) by a separate license from Life Technologies Corporation. For licensing information, please contact outlicensing@lifetech.com or Out Licensing, Life Technologies, 5791 Van Allen Way, Carlsbad, California Trademarks Fluidigm, the Fluidigm logo, Access Array, Biomark, EP1, Digital Array, Dynamic Array, FC1, Flex Six, MSL, NanoFlex, qdpcr 37K, SNP Type, and Delta Gene are trademarks or registered trademarks of Fluidigm Corporation in the United States and/or other countries. All other trademarks are the sole property of their respective owners. For EU's WEEE directive information, go to fluidigm.com/compliance 2015 Fluidigm Corporation. All rights reserved. 05/2015 For technical support visit fluidigm.com/support United States techsupport@fluidigm.com Europe techsupporteurope@fluidigm.com Asia techsupportasia@fluidigm.com Latin America techsupportlatam@fluidigm.com All other countries techsupport@fluidigm.com PHONE United States (toll-free) Europe Japan China (excluding Hong Kong) All other countries

3 Contents About This Guide 5 How to Use This Guide 5 Safety Alert Conventions 5 Safety Alerts for Chemicals 5 Safety Alerts for Instruments 6 Safety Data Sheets 6 Related Documents 7 Chapter 1: Introducing IFC Controller WX 8 Components of the IFC Controller WX 9 Workflow for Reusable IFCs 10 Process Overview 11 Technical Specifications 11 Operating Environment 11 Noise Level 11 Regulatory Compliance 11 Power Options 12 Barcode Labeling 12 Chapter 2: IFC Controller Basics 13 Start the IFC Controller 13 Load an IFC 14 Run Scripts 15 Chapter 3: Using the IFC Controller Software 17 Log In 17 User Tools 18 Administrator Tools 19 Edit User 19 Change Settings 20 Operating in Group Mode 21 Clean System (manually) 24 Network Settings 24 Manage Users 25 Export Logs 27 Change Time/Date 27 Manage Scripts 28 Log Out 28 Update Software and Scripts 29 Install Updated Software and Scripts 29 Load New Scripts 30 Appendix A: Troubleshooting and Maintenance 32 Observation and Possible Course of Action 32 Clean the IFC Controller 33 Clean the Interface Plate 33 Clean the System 35 Manually Clean the System 36 Fuse Replacement 38 Appendix B: IFC Controller Site Requirements and Setup 40 Site Requirements 40 Proper Grounding/Protective Earth Connection 40 Electrical Requirements 40 Uninterruptible Power Supply 41 Workspace 41 Electrical Connection 42 Unpack the IFC Controller 43 Install the IFC Controller 43 Connect the Power Cable 44 Power On the IFC Controller 44 Appendix C: Safety 45 General Safety 45 3

4 Contents Instrument Safety 45 Instrument Symbols 46 Chemical Safety 47 Disposal of Products 47 4

5 About This Guide CAUTION ABBREVIATED SAFETY ALERTS. Hazard symbols and hazard types specified in procedures may be abbreviated in this document. For complete safety information, see the safety appendix on page 53. How to Use This Guide This user guide provides information about setup, installation, operation, and maintenance of the IFC controller. In this document, the term controller refers to the IFC controller and the term software refers to the IFC controller software. Safety Alert Conventions This guide uses specific conventions for presenting information that may require your attention. Refer to the following safety alert conventions. Safety Alerts for Chemicals Fluidigm follows the United Nations Globally Harmonized System (GHS) for communicating chemical hazard information. GHS provides a common means of classifying chemical hazards and a standardized approach to chemical label elements and safety data sheets (SDSs). Key elements include: Pictograms that consist of a symbol on a white background within a red diamondshaped frame. Refer to the individual SDS for the applicable pictograms and warnings pertaining to the chemicals being used. Signal words that alert the user to a potential hazard and indicate the severity level. The signal words used for chemical hazards under GHS: DANGER Indicates more severe hazards. WARNING Indicates less severe hazards. 5

6 About This Guide Safety Data Sheets Safety Alerts for Instruments For hazards associated with instruments, this guide uses the following indicators: Pictograms that consist of a symbol on a white background within a black triangleshaped frame. Signal words that alert the user to a potential hazard and indicate the severity level. The signal words used for instrument hazards: DANGER Indicates an imminent hazard that will result in severe injury or death if not avoided. WARNING Indicates a potentially hazardous situation that could result in serious injury or death. CAUTION Indicates a potentially hazardous situation that could result in minor or moderate personal injury. IMPORTANT Indicates information necessary for proper use of products or successful outcome of experiments. Safety Data Sheets Read and understand the SDSs before handling chemicals. To obtain SDSs for chemicals ordered from Fluidigm Corporation, either alone or as part of this system, go to fluidigm.com/sds and search for the SDS using either the product name or the part number. Some chemicals referred to in this user guide may not have been provided with your system. Obtain the SDSs for chemicals provided by other manufacturers from those manufacturers. 6

7 About This Guide Related Documents Related Documents This document is intended to be used in conjunction with these related documents: Document Title Part Number Biomark HD Data Collection User Guide Biomark/EP1 Data Collection User Guide SNP Genotyping Analysis User Guide Genotyping with the FR48.48 IFC Using SNP Type Assays Genotyping with the FR48.48 IFC Using Standard TaqMan Assays Cleaning the FR48.48 IFC (Wash and Bake)

8 Chapter 1: Introducing IFC Controller WX IFC controller WX is a compact single-bay instrument that employs pneumatic pressure to precisely meter samples and reagents and to control valves within the FR48.48 Dynamic Array integrated fluidic circuit (IFC). The IFC controller can control and apply pressures to different sealing zones on the IFC input/output frame to enable: Metering of liquids from inlet positions in the input frame to reaction chambers within the IFC Metering of liquids within the IFC Opening and closing of valves within the IFC IFC controller WX has a heated anvil in the IFC tray that allows liquids to evaporate from IFC wells when purge scripts are run. CAUTION Be careful not to touch the heated anvil after a purge script has been run or if a purge script is aborted. The anvil is normally not hot enough to cause injury, but if the IFC is ejected during a purge script, the anvil may be as hot as 90 ºC. Heated anvil Hot surface hazard symbol Warning message after purge This user guide walks you through: Key features and benefits of IFC controller WX Installation and setup procedures Description of system functions NOTE IFC controller WX is for research use only and is not for use in diagnostic procedures. 8

9 Chapter 1: Introducing IFC Controller WX Components of the IFC Controller WX Components of the IFC Controller WX LCD touchscreen Interface plate Reusable IFC Tray Figure 1. Front of IFC controller On/off switch Power cord input socket Fuse label Networking ports USB port Figure 2. Back of IFC controller 9

10 Chapter1:Introducing IFC Controller WX Workflow for Reusable IFCs Component Purpose Quantity IFC controller Controls filling of reagents and valve actuation in IFCs 1 Removable interface plate Power cable IFC controller WX User Guide Interface between IFC and IFC controller with pressure inlet ports Country-specific power cable to connect IFC controller to wall socket Describes in detail how to install and use the IFC controller Fuse Spare fuse based on country voltage 2 Removable interface plate O-rings Spares for use if an O-ring is lost during cleaning of interface plate 4 NOTE To order additional items, contact Fluidigm sales. For phone or contact information, go to fluidigm.com/contact Workflow for Reusable IFCs Equipment required: 2 IFC controller WXs: one for loading only and one for washing only FC1 cycler for fast thermal cycling EP1 for end-point SNP genotyping runs Lab Companion convection oven for drying reusable IFCs The workflow is illustrated in the process below. he EP1 1 Prime the FR48.48 IFC in the Load-only WX 2 Prepare assays, sample pre-mix and samples 3 Load the FR48.48 IFC in the Load-only WX. 4 Thermal cycle the FR48.48 IFC in FC1 cycler 5 Collect data on EP1 6 Clean the FR48.48 IFC in the Clean-only WX 7 Dry the FR48.48 IFC in the convection oven For more information, see the SNP Genotyping Analysis User Guide (PN ). 10

11 Chapter 1: Introducing IFC Controller WX Process Overview Process Overview Stop Reuse IFCs Yes No PCR 5? Wash 1 Wash 2 New IFC Prime Load Bake dry Purge Technical Specifications Electrical input voltage of the IFC Controller WX is VAC, 2.7A, 50/60Hz. Operating Environment IFC controller WX should be used in an environment that meets the following requirements. IMPORTANT The IFC controller is for indoor use only. Temperature Ambient between 20 ºC and 30 ºC Humidity 30% 80%, noncondensing Pollution Degree 2 Electrical Installation Altitude Category II Up to 2,000 m Noise Level The operation of IFC Controller WX emits a noise level of 64 db. Regulatory Compliance IFC controller WX is CE compliant for laboratory equipment and electrical safety. 11

12 Chapter1:Introducing IFC Controller WX Power Options Power Options IFC controller WX operates through V AC power at 50/60 Hz. Barcode Labeling Labels on the IFC contain the Fluidigm logo, a unique 10-character number, and a one-dimensional, Code 128 barcode. You can use this barcode with the provided integrated barcode reader to track samples and experiments. 12

13 Chapter 2: IFC Controller Basics This chapter describes how to use IFC controller WX. IMPORTANT To set up the IFC controller, read the site requirements and installation instructions in Appendix B: IFC Controller Site Requirements and Setup on page 40. CAUTION LIFTING HAZARD. The IFC controller is shipped in one cardboard box containing the controller, power cables, and spare fuses. It weighs 59 lb (27 kg). Use caution when moving the shipping box. Start the IFC Controller 1 Turn on the switch on the back of the IFC controller. On/off switch 2 The LCD display lights up. After seconds the initialization screen appears. The IFC controller software starts and initializes all system components. After initialization, the instrument runs an internal health check. In the event an error occurs, contact technical support for service. For phone or contact information, see page 2. 13

14 Chapter2:IFC Controller Basics Load an IFC Load an IFC For more information about using the software, see Chapter 3: Using the IFC Controller Software. 1 At completion of power-up and instrument initialization, the Home screen appears. You may log in now or later. See Log In on page Press Eject to move the tray out of the IFC controller. 3 Place the IFC onto the tray by aligning its notched corner to the A1 mark. Remove and discard the blue protective film from the bottom of the IFC, if you have not already done so. A1 mark Notched corner on IFC 4 Press Load Chip to register the IFC barcode and activate script selection. 14

15 Chapter2:IFC Controller Basics Run Scripts Run Scripts 1 Choose a script to run from the script selection window. The list of available scripts is defined by the IFC barcode. This example uses Prime (168x). IFC icon and barcode Load Mix (168x) If prompted to log in, see Log In on page Press Run Script. Prime (168x) 3 A status screen appears with a time estimate for completion. Pressing Abort during an active script will cancel the run. 15

16 Chapter2:IFC Controller Basics Run Scripts When the script has successfully completed, a dialog box alerts you that the script is completed and displays an Eject button. 4 Press Eject to move the IFC out of the controller. 5 You may now pipet into the IFC. 6 Return the IFC to the IFC controller WX. 7 Run the appropriate script to introduce samples and primers into the IFC and to clean the IFC. (168x) 16

17 Chapter 3: Using the IFC Controller Software Log In 1 Press Login at the bottom right of the screen. 2 Log in as Admin or User. When entering a password for the first time: If you are logging in as Admin, the initial default password is: If you are logging on as User, there is no initial default password. For more information, see Manage Users on page

18 Chapter 3: Using the IFC Controller Software User Tools User Tools 1 Press Tools at the bottom right of the screen. When logged in as User, these tools are available: Edit User. Allows you to change your password. Settings. Allows you to enable full logging for this session. If this option is selected, the system logs every event on the system until it is rebooted or the option is turned off. This is useful if you believe your machine is not functioning correctly. You can record what your machine is doing and export the log and share it with Fluidigm technical support for troubleshooting. By default, this option is not selected. Clean System. Allows you to manually clean the system. For more information, see Manually Clean the System on page 36. Export Log. Allows you to export system activity logs if you selected Enable Full Logging for this Session on the Settings screen. For more information, see Export Logs on page

19 Chapter 3: Using the IFC Controller Software Administrator Tools Administrator Tools When logged in as Administrator you can manage users, passwords, settings, and scripts. 1 Press Tools at the bottom right of the screen. The Tools menu appears: Edit User On the Edit User screen, you can edit user name, password, and notification by clicking the corresponding Edit buttons. 19

20 Chapter 3: Using the IFC Controller Software Administrator Tools If you edit notification, a new screen appears. You can receive notifications by or by text message. If you select By Phone Number and Carrier, you also have to select the carrier service that you subscribe to. Change Settings 1 Press Settings. Settings on this screen include: Enable Full Logging for this Session. If this option is selected, the system logs every event on the system until it is rebooted or the option is turned off. This is useful if you believe your machine is not functioning correctly. You can record what your machine is doing and export the log to share with Fluidigm technical support for troubleshooting. By default, this option is turned off. Enable Barcode. Selected by default. The barcode reader attempts to read the barcode of every IFC. If you are running an IFC that does not have a barcode, you can turn off this setting. We recommend that you keep the barcode reader enabled. The instrument will display only those scripts that pertain to your IFC type. This helps prevent errors in script selection. 20

21 Chapter 3: Using the IFC Controller Software Administrator Tools 2 Press Save if you change the settings. WX Operating in Group Mode You can operate the IFC controller WX in conjunction with other IFC controllers. 1 Select This instrument is the group master on the instrument you wish to use as the master controller. 2 On the instruments you wish to link to the master controller, open Group Mode Settings and select This instrument is the group worker. 3 Return to the master IFC controller and select Add More Workers. 21

22 Chapter 3: Using the IFC Controller Software Administrator Tools 4 Select the controllers to link. WX wx-1 5 Click Add Workers. The master controller now has a Master Mode icon on its screen and the worker controller(s) now have a Worker Mode icon on their screen. Master mode Worker mode You can now control the worker IFC controllers remotely via the master IFC controller. 22

23 Chapter 3: Using the IFC Controller Software Administrator Tools 6 Select the number of IFCs you wish to run. The default number is the number of IFC controllers connected via group operating mode. For example, if you have four IFC controllers linked, four will be the default number of IFCs to run. If you only want to run two, select 2 to adjust the number. Master mode WX-1 To leave the worker or master modes, click Exit Mode. 7 You can also operate a system independently by selecting This instrument is independent. The screen indicates it is in worker mode, independent of any other systems: WX-1 23

24 Chapter 3: Using the IFC Controller Software Administrator Tools Clean System (manually) 1 Press Clean System. 2 Place the cleaning plate on the tray and press Start System Cleaning. 3 When the system purge is complete, the tray ejects. For more details, see Manually Clean the System on page 36. Network Settings WX-1 Database address On the Network Setting tab, your IT professional can network the IFC controller to your company s network and obtain an IP address dynamically through DHCP or use a static IP address. Other editable items are: DNS addresses Network unit name If you are networking multiple IFC controllers, you need to edit the network unit name. Controllers are delivered with the same default name. Database address 24

25 Chapter 3: Using the IFC Controller Software Administrator Tools On the Server tab, editable items include: Mail server Port number User name Password From Manage Users Use the Manage Users screen to add, delete, and edit users. 1 Press Manage Users. 2 Press Add New User. 3 Use the onscreen keyboard to enter a user name. 4 Press Next. 25

26 Chapter 3: Using the IFC Controller Software Administrator Tools 5 To assign a password for this user, press Change. Enter a password using the onscreen keyboard. 6 Assign an access level by choosing Normal or Administrator. Normal access level allows basic script running. Administrator access level allows basic script-running and the ability to change user access and passwords and manage scripts and system settings. 7 Press Save. The new user name appears on the login screen. 26

27 Chapter 3: Using the IFC Controller Software Administrator Tools Export Logs If you enabled Full Logging for this Session in the Settings screen, you can export system logs to a USB drive. 1 Press Export Logs from the Tools menu. 2 Insert your USB drive into the USB port on the back of the instrument. 3 Press Export. Change Time/Date 1 Press Change Time/Date. Use the blue arrows to adjust the time and date from this screen. 2 Press Save when finished. 27

28 Chapter 3: Using the IFC Controller Software Log Out Manage Scripts For more detailed information on loading and updating scripts, see Update Software and Scripts on page Press Manage Scripts. 2 Add scripts from a USB drive or copy scripts to a USB drive from this screen. Your scripts must be in a folder named Scripts on your USB drive for the IFC controller to find them. 3 Press Save. Scripts in the IFC Controller WX Software The available IFC controller scripts are: Script Load Mix (168x) Description Pressurizes the primer and sample into the reaction chambers and closes the containment valves at the end. Log Out 1 When you are finished using the IFC controller, press Logout at the bottom right of the screen. 2 Press Log Off. 28

29 Chapter 3: Using the IFC Controller Software Update Software and Scripts Update Software and Scripts Occasionally, Fluidigm provides updates to the system software and new scripts. Check for updates on our website at fluidigm.com/software Install Updated Software and Scripts 1 Turn off IFC controller WX by pressing the power switch at the back of the instrument. 2 Insert the USB drive containing the updates into the USB port. 3 Turn on IFC controller WX by pressing the power switch at the back of the instrument. 4 Press Update All to update the software and firmware. You can also update only the firmware or only the software. IFC Update Utility UPDATE ALL UPDATE FIRMWARE UPDATE SOFTWARE A status screen indicates the software/firmware was successfully updated. The instrument was successfully updated Please remove the USB Key and reboot the instrument to complete the update. 5 Turn off the IFC controller, take out the USB drive, and turn the controller back on to reboot the system. 29

30 Chapter 3: Using the IFC Controller Software Update Software and Scripts Load New Scripts 1 Log in as Administrator. 2 Press Tools. 3 Press Manage Scripts. 4 To add all of the new scripts, press Add All Scripts from USB Key. Load Mix (168x) You can now view, rename, or delete the individual scripts. To select a script, press the script s name. This example shows Load Mix (168x). Load Mix (168x) 30

31 Chapter 3: Using the IFC Controller Software Update Software and Scripts You can now change its name or delete it. Load Mix (168x) Load Mix 5 To change the name of the selected script, press Change and enter a new name. 6 To delete unwanted scripts, press Delete Script. 31

32 Appendix A: Troubleshooting and Maintenance Observation and Possible Course of Action Observation Possible Cause Recommended Action IFC controller WX failed to power on. There is no display after power is turned on. Compression force is out of range. Error messages Pump timed-out error IFC load error/cannot read barcode No AC power IFC controller WX fuse is faulty IFC controller WX power supply is not fully reset The compression cylinder pressure is out of range One or more of the O-rings may be unseated Blue protective film may not have been removed from bottom of IFC Check that the power cable is properly connected to both wall socket and IFC controller WX. Make sure the power switch on wall socket is on, and then turn on the instrument power switch on the back panel. If using a power strip, make sure it is turned on. Refer to Fuse Replacement on page 38 and replace both fuses with the supplied replacement fuses. Turn off the IFC controller WX power switch located on the back panel. Wait for 10 seconds and re-boot it by turning on the power switch. Call technical support. Reboot the system. If the system fails to recover, call technical support. Check interface plate O-rings. (See Clean the Interface Plate on page 33.) Ensure blue protective film is removed from the bottom of the chip before loading into the IFC controller WX. 32

33 Appendix A: Troubleshooting and Maintenance Clean the IFC Controller Clean the IFC Controller IFC controller WX requires very little maintenance other than regular cleaning of the IFC loading bay and the removable interface plate. With reusable IFCs, perform the system clean and also clean the interface plate before every IFC run on both the Load and Wash IFC controllers. You can run the cleaning protocol manually by accessing the Clean System script in the Tools menu. Clean the Interface Plate 1 After the tray ejects, remove the interface plate. Removable interface plate 2 Using a lint-free cloth and DNAZap (Life Technologies, PN AM9890), gently wipe the top and bottom of the interface plate. 33

34 Appendix A: Troubleshooting and Maintenance Clean the IFC Controller 3 Use clear tape to remove any dust particles. 4 Reinstall the cleaned removable interface plate: a b Orient the plate so that THIS SIDE UP is facing up. Slide the plate into the guide until it stops and you hear a click. IMPORTANT There are eight black O-rings inserted on one side of the removable interface plate. Be sure they are not misplaced or removed while handling the plate. During interface plate installation, these O-rings should face up. Contact technical support if you need replacement O-rings. 5 Press Done when finished. 34

35 Appendix A: Troubleshooting and Maintenance Clean the IFC Controller Clean the System Follow the onscreen instructions: 1 Press Clean. The System Cleaning Instructions screen appears. 2 Place cleaning plate onto tray. 35

36 Appendix A: Troubleshooting and Maintenance Clean the IFC Controller 3 Press Start System Cleaning. A status screen appears: 4 When the system purge is complete, eject the tray. 5 Wipe the tray out with water and let it dry for its next use. Manually Clean the System You can manually clean the system at any time. 1 Eject the IFC. 2 Press Tools. 3 Press Clean System. The System Cleaning Instructions screen appears. 36

37 Appendix A: Troubleshooting and Maintenance Clean the IFC Controller 4 Place the cleaning plate on the tray and press Start System Cleaning. A status screen appears. 5 When the system purge is complete, the tray ejects. 37

38 Appendix A: Troubleshooting and Maintenance Fuse Replacement Fuse Replacement No fuse replacement is required during installation. If the power fuse is faulty, replace it as follows. The fuse is rated 250V / 3.15A. 1 Turn off and unplug power cord from wall outlet. 2 Remove fuse casing from the IEC inlet power switch. 3 Push and release fuse holder blocks as shown below. 38

39 Appendix A: Troubleshooting and Maintenance Fuse Replacement 4 Replace faulty fuses and reinstall the IEC filter. Follow the arrow marks in the fuse holder to match IEC casing. Arrow marks 39

40 Appendix B: IFC Controller Site Requirements and Setup CAUTION The IFC controller is shipped in one cardboard box containing the controller, power cables, and spare fuses. It weighs 59 lb (27 kg). Use caution when moving the shipping box. CAUTION The IFC controller must be installed and operated in accordance with Fluidigm recommendations. Failure to follow these instructions may invalidate your warranty and/or diminish the safe functioning of your instrument. Site Requirements To operate the IFC controller, your site should meet the following requirements: One electrical power outlet A workspace that accommodates the IFC controller Proper Grounding/Protective Earth Connection To reduce the risk of electrical shock, connect the IFC controller to the AC power main through a grounded power cable at the power outlet. Electrical Requirements The IFC controller should be plugged into a standard US-type power strip with surge protector that provides single-phase AC voltage, between 100 and 240 V (60 or 50 Hz). 40

41 Appendix B: IFC Controller Site Requirements and Setup Site Requirements Uninterruptible Power Supply If your local power supply is subject to frequent power interruptions, you can protect your experiment setups on the IFC controller by using an uninterruptible power supply (UPS) with these minimum requirements: Output Power Capacity Backup Time (Run-Time) Output Voltage 500W (800VA) ±30 minutes at 500W VAC Output Connection NEMA 5-15R The UPS described above is sufficient to maintain power to one IFC controller. Workspace Provide a work surface that can accommodates the IFC controller. The following table shows the instrument dimensions and weight. Length Width Height Weight 19 in (48.3 cm) 9.5 in (24.1 cm) 13 in (33 cm) 59.4 lb (27 kg) The IFC controller is designed as desktop equipment. It can be placed on a laboratory bench top. To accommodate one IFC controller, we recommend you supply a bench top with minimum depth of 25 in (63.5 cm) and width of 16 in (40.6 cm). To allow adequate air circulation, keep at least 2 in (5.08 cm) clearance behind the IFC controller. Multiple controllers can be stacked together side by side. IMPORTANT Do not place the IFC controller on a heated surface or directly above a source of heat. Do not place the IFC controller in a position that makes it difficult to operate or disconnect from power. 41

42 Appendix B: IFC Controller Site Requirements and Setup Electrical Connection 13 in (33 cm) 9.5 in (24.1 cm) 2 in (5.1 cm) clearance 6 in (15.2 cm) Figure 3. IFC controller dimensions 19 in (48.3 cm) 25 in (63.5 cm) Electrical Connection The IFC controller is a standalone desktop instrument that requires only one electrical connection (Figure 4) at the back of the IFC controller. The top left corner has an AC power input socket and a power on/off switch. On/off switch Power cord Figure 4. Electrical connection 42

43 Appendix B: IFC Controller Site Requirements and Setup Unpack the IFC Controller Unpack the IFC Controller CAUTION LIFTING HAZARD. The IFC controller is shipped in one cardboard box that also contains a power cable and cleaning plates; together they weigh 59 lb (27 kg). Use proper lifting techniques. We recommend using at least two people to lift the IFC controller from the box. 1 Unpack the box near its permanent place in the lab. 2 Remove components from plastic wrapping. 3 Confirm that IFC controller, power cable, and cleaning plates are included in the box. If anything is missing, contact technical support. For phone or contact information, see page 2. Install the IFC Controller IMPORTANT Do not place the IFC controller on a heated surface or directly above a heat source. 1 Place the IFC controller on a laboratory bench top. For more details, see Workspace on page Provide at least 2 in (5.1 cm) of clearance behind the controller to allow adequate air circulation. See Figure 3 on page

44 Appendix B: IFC Controller Site Requirements and Setup Connect the Power Cable Connect the Power Cable 1 Connect the power cable to the IFC controller. On/off switch Power cord 2 Plug in the IFC controller to an electrical outlet that provides single-phase AC voltage, between 100 and 240 volts (50 or 60 Hz). Make sure all power switches are off. No additional grounding is required. IMPORTANT Current requirement is 5 amps, but the outlets must be of US standard (NEMA 1-15P) blade type. If you are not operating in the United States, Fluidigm provides a country-specific power cord. If your local power supply is subject to frequent power interruptions, you may protect your IFC runs on the IFC controller by using an uninterruptible power supply (UPS). For more information, see Uninterruptible Power Supply on page 41. Power On the IFC Controller 1 If you are using a surge protector power strip, turn it on. 2 Turn on the power switch at the back of the instrument. On/off switch 3 Wait while the IFC controller initializes. Initialization may take up to 5 minutes to complete. The IFC controller uses a touchscreen interface. Once you power on the instrument, all interactions with the application occur by touching the screen. 44

45 Appendix C: Safety General Safety In addition to your site-specific safety requirements, Fluidigm recommends the following general safety guidelines in all laboratory and manufacturing areas: Use personal protective equipment (PPE): safety glasses, fully enclosed shoes, lab coats, and gloves. Know the locations of all safety equipment (fire extinguishers, spill kits, eyewashes/ showers, first-aid kits, safety data sheets, etc.), emergency exit locations, and emergency/injury reporting procedures. Do not eat, drink, or smoke in lab areas. Maintain clean work areas. Wash hands before leaving the lab. Instrument Safety WARNING Do not modify this device. Unauthorized modifications may create a safety hazard. WARNING BIOHAZARD. If you are putting biohazardous material on the instrument, use appropriate personal protective equipment and adhere to Biosafety in Microbiological and Biomedical Laboratories (BMBL) from the Centers for Disease Control and Prevention and to your lab s safety protocol to limit biohazard risks. If biohazardous materials are used, properly label the equipment as a biohazard. For more information, see the BMBL guidelines at: cdc.gov/biosafety/publications/index.htm 45

46 Appendix C: Safety Instrument Safety Instrument Symbols Symbol English Français Caution, risk of danger Consult the manual for further safety information. Caution, hot surface Attention, risque de danger Consulter le manuel pour d autres renseignements de sécurité. Attention, surface chaude Pinch hazard Risque de pincement Biohazard Risque biologique On/off On/off (marche/arrêt) Protective conductor terminal (main ground). It must be connected to earth ground before any other electrical connections are made to the instrument. To minimize negative environmental impact from disposal of electronic waste, do not dispose of electronic waste in unsorted municipal waste. Follow local municipal waste ordinances for proper disposal provisions and contact customer service for information about responsible disposal options. Borne de conducteur de protection (mise à la terre principale) Pour minimiser les conséquences négatives sur l environnement à la suite de l élimination de déchets électroniques, ne pas éliminer ce déchet électronique avec les déchets usuels non soumis au tri sélectif. Se conformer aux ordonnances locales sur les déchets municipaux pour les dispositions d élimination et communiquer avec le service à la clientèle pour des renseignements sur les options d élimination responsable. Conformity mark Description Indicates conformity with safety requirements for Canada and the United States. Indicates conformity with European Union requirements for safety and electromagnetic compatibility. 46

47 Appendix C: Safety Chemical Safety Chemical Safety Read and comprehend all safety data sheets (SDSs) by chemical manufacturers before you use, store, or handle any chemicals or hazardous materials. Wear personal protective equipment (gloves, safety glasses, fully enclosed shoes, lab coats) when handling chemicals. Do not inhale fumes from chemicals. Use adequate ventilation, and return caps to bottles immediately after use. Check regularly for chemical spills or leaks. Follow SDS recommendations for cleaning up spills or leaks. Disposal of Products Used IFCs should be handled and disposed of in accordance with federal, state, regional, and local laws for hazardous waste management and disposal. Do not dispose of this product in unsorted municipal waste. This equipment may contain hazardous substances that could affect health and the environment. Use appropriate take-back systems when disposing of materials and equipment. Learn more at fluidigm.com/compliance 47

48 Appendix C: Safety Disposal of Products 48

49 For technical support visit fluidigm.com/support