Customer Responsibilities

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1 Thank you for purchasing an Agilent instrument. To get you started and to assure a successful and timely installation, please refer to this specification or set of requirements. Correct site preparation is the key first step in ensuring that your instruments and software systems operate reliably over an extended lifetime. This document is an information guide AND checklist prepared for you that outlines the supplies, consumables, space and utility requirements for your equipment for your site. Customer Responsibilities Make sure your site meets the following prior specifications before the installation date. For details, see specific sections within this checklist, including: One Set of ScreenTape assay consumables including ScreenTape devices, ladder and reagents must be purchased by the customer and must be available. This needs to be confirmed before installation date as the installation and familiarization service delivery includes a demonstration of an actual analysis with ladder. Any Agilent ScreenTape assay can be used for the installation and familiarization service delivery. Please lookup available kits in the section Required Operating Supplies by customer. For installation and familiarization with the D1000 ScreenTape assay the following items should be order and present (kit type is an example only). o D1000 ScreenTape devices: (includes 7 devices, of which 1 will be used partially) o D1000 Reagents: (includes ladder & sample buffer, used only partially) The necessary laboratory or bench space is available, see Dimensions and Weight section. The environmental conditions for the lab are met, see Environmental conditions section. See Power Consumption section for related requirements in number & location of electrical outlets The Required Operating Supplies necessary for the product and installation If Agilent is delivering installation and familiarization services, users of the instrument should be present throughout these services; otherwise, they will miss important operational and safety information. Issued: 30-Sept 2015 Revision: 01 Copyright Agilent Technologies For Research Use Only. Not for use in diagnostic procedures. Page 1 of 8

2 Important Customer Information 1. If you have questions or problems in providing anything described as a Customer Responsibility above, please contact your local Agilent or partner support/service organization for assistance prior to delivery. In addition, Agilent and/or its partners reserve the right to reschedule the installation dependent upon the readiness of your laboratory. 2. Should your site not be ready for whatever reason, please contact Agilent as soon as possible to rearrange any services that have been purchased. 3. Other optional services such as additional training and consultation for user-specific applications may also be provided at the time of installation when ordered with the system, but are contracted separately. Please note: For Research Use Only. Not for use in diagnostic procedures. Issued: 30-Sept 2015 Revision: 01 Copyright Agilent Technologies For Research Use Only. Not for use in diagnostic procedures. Page 2 of 8

3 Dimensions and Weight Identify the laboratory bench space before your system arrives based on the table below. Pay special attention to the total height and total weight requirements for all system components you have ordered and avoid bench space with overhanging shelves. Also pay special attention to the total weight of the modules you have ordered to ensure your laboratory bench can support this weight. The work bench should be stable and vibration free. Special Notes Instrument Description Weight Height Depth Width Kg lbs cm in cm in cm in 4200 TapeStation instrument 25.5 kg 56.3 lbs PC/ Laptop (typical values) Please note: Additional space of approximately 25 cm is required on top of the listed height in order to allow opening of the 4200 TapeStation lid. Please avoid locations with low overhanging shelves. Please note: PC/ Laptop dimensions are subject to changes as the PC/laptop bundled into the 4200 TapeStation system itself is. Please note: The 4200 TapeStation instrument is delivered in a cardboard box mounted on an 80x60cm pallet with a total height of approximately 80 cm. Make sure sufficient storage space is available prior to installation. Issued: 30-Sept 2015 Revision: 01 Copyright Agilent Technologies For Research Use Only. Not for use in diagnostic procedures. Page 3 of 8

4 Environmental Conditions Operating your instrument within the recommended temperature ranges ensures optimum instrument performance and lifetime. Special Notes Performance can be affected by sources of heat & cold e.g. direct sunlight, heating/cooling from air conditioning outlets, drafts and/or vibrations. The site s ambient temperature conditions must be stable for optimum performance, see table below. Do not operate the 4200 TapeStation instrument outside the temperature range of C ( F). Please note the ScreenTape assays do have individual Operating temp ranges deviating from the instruments range. Allow approximately 75mm (3 inches) of clear space around the instrument for air circulation and electrical connections Do not operate the 4200 TapeStation instrument outside the range of 15-80% relative humidity. Keep the conditions non- condensing. Upon delivery, store in a temperature stable, dry environment. Do not remove the instrument from its original shipping box immediately, but allow it to warm up to the ambient temperature of the operating environment. High levels of ambient light can affect the instruments performance. Position the 4200 TapeStation instrument facing away from direct light sources. Placed the instrument on a flat and stable surface to avoid spillage of solutions and to achieve accurate results. To avoid damaging the instrument and to achieve accurate results do not place the 4200 TapeStation instrument on a vibrating surface or near vibrating objects Ensure that nothing rests on the cables of the 4200 TapeStation instrument and that they are not located where they can be stepped on or tripped over Instrument Description Operating Temperature Range Operating Humidity Range 4200 Tape Station system C ( F) 15-80% rh, non-condensing Issued: 30-Sept 2015 Revision: 01 Copyright Agilent Technologies For Research Use Only. Not for use in diagnostic procedures. Page 4 of 8

5 Assay Operating temp range C (F) D1000 ScreenTape assay High Sensitivity D1000 ScreenTape Genomic DNA ScreenTape RNA and High Sensitivity RNA ScreenTape D5000 ScreenTape assay C (54 99 F) C (63 99 F) 15 C to 30 C (58-86 F) 14 C to 30 C (57-86 F) 15 C to 30 C (59-86 F) Power Consumption Special Notes A computer system is supplied with your instrument. Account for respective electrical outlets. 2 plug sockets must be available near the allocated bench space Instrument PC/ Laptop Instrument Description Line Voltage & Frequency (V, Hz) Maximum operting Power Consumption (VA) Agilent 4200 TapeStation instrument V AC, 50-60Hz 50W (VA) PC/Laptop, bundled with instrument V AC, 50-60Hz Typically W Please note: PC/ Laptop power consumption through HP Smart AC Adapter is subject to changes as the PC/laptop bundled into the 4200 TapestStation System itself is. Issued: 30-Sept 2015 Revision: 01 Copyright Agilent Technologies For Research Use Only. Not for use in diagnostic procedures. Page 5 of 8

6 Required Operating Supplies by Customer Special Notes: ScreenTape devices and associated reagents are not provided with the 4200 TapeStation instrument delivery and must be purchased separately. Please note the reagent specific storage conditions upon their arrival. ScreenTape devices and reagents for the chosen assay must be purchased by the customer prior to the installation. One ScreenTape device, ladder and reagents must be provided by the customer for installation and familiarization at the installation. Tube strips, tube caps and loading tips do come with the system and are partially used during installation and familiarization. The following table lists the available consumables, by application, and suggested apparatus required for each assay. All All Application Item Description Suggested Vendor s Part Number (if applicable) RNA, HS RNA, D1000, HS D1000 All All RNA Applications Generic mixing device. (recommended, not supplied) Volumetric pipette and suitable pipette tips for volumes 1 to 10 µl IKA MS3 Vortex (supplied with instrument as delete option) Centrifuge to fit sample tube strips, allowing spin down at 500g for 10s. (recommended, not supplied) Centrifuge to fit 96 well plates, allowing spin down at 500g for 10s. (recommended, not supplied) Block Heater or PCR instrument with lid heating fitting to 200µl vials IKA None - - None - Centrifuge with 8-Tube strip rotor e.g. Labnet C Microplate centrifuge, PCR Plate Spinner e.g. VWR None - 7 x D1000 ScreenTape devices D1000 ScreenTape assay 1 x D1000 Reagents 1 x D1000 Sample Buffer 1 x D1000 Ladder D1000 Ladder Issued: 30-Sept 2015 Revision: 01 Copyright Agilent Technologies For Research Use Only. Not for use in diagnostic procedures. Page 6 of 8

7 Table continued Application Item Description Suggested Vendor s Part Number (if applicable) High Sensitivity D1000 ScreenTape assay 7 x High Sensitivity D1000 ScreenTape devices 1 x High Sensitivity D1000 Reagents 1 x HS D1000 Sample Buffer 1 x HS D1000 Ladder x High Sensitivity D1000 Ladder x Genomic DNA ScreenTape devices Genomic DNA ScreenTape assay 1 x Genomic DNA Reagents 1 x Genomic DNA Sample Buffer 1 x Genomic DNA Ladder x RNA ScreenTape devices RNA ScreenTape assay 1 x RNA ScreenTape Sample Buffer x RNA ScreenTape Ladder High Sensitivity RNA ScreenTape assay 7 x HS RNA ScreenTape devices x HS RNA ScreenTape Sample Buffer x HS RNA ScreenTape Ladder x D5000 ScreenTape devices D5000 ScreenTape assay 1 x D5000 ScreenTape Reagents 1 x D5000 Sample Buffer x D5000 Ladder 1 x D5000 ScreenTape Ladder x High Sensitivity D5000 ScreenTape devices High Sensitivity D5000 ScreenTape assay 1 x High Sensitivity D5000 Reagents 1 x HS D5000 Sample Buffer x HS D5000 Ladder 1 x HS D5000 ScreenTape Ladder Issued: 30-Sept 2015 Revision: 01 Copyright Agilent Technologies For Research Use Only. Not for use in diagnostic procedures. Page 7 of 8

8 Important Customer Web Links For additional information about our solutions, please visit our web site at Specific 4200 TapeStation information - Literature Library - Find your local phone numbers and mail addresses Document part number: G Issued: 30-Sept 2015 Revision: 01 Copyright Agilent Technologies For Research Use Only. Not for use in diagnostic procedures. Page 8 of 8

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