Performance Simplicity Elegancy

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1 Performance Simplicity Elegancy The new TProfessional Thermocycler Family

2 Te c h n i c a l S p e c i f i c a t i o n a n d O r d e r i n g I n f o r m a t i o n TProfessional TProfessional Basic Block High Speed Silver Block Gold anodized 96 well block Max Heating 5.0 C/sec* 3 C/sec Max Cooling 3.5 C/sec* 2 C/sec Temp Uniformity +/ C within 15 sec at 55 C +/ C within 15 sec at 70 C +/ C within 15 sec at 95 C +/ C within 15 sec at 55 C +/ C within 15 sec at 70 C +/ C within 15 sec at 95 C Temp Gradient** 40 C 26 C Temp Range 3 C to 99 C 3 C to 99 C Control Accuracy +/- 0.1 C +/- 0.1 C Block Formats 60 well / 96 well / 384 well*** 96 well Block exchange Within 10 seconds - Programming modes Spread sheet plus graphical Spread sheet Software options Quickstart of the last five programs, program preview prior to start, toggle between spreadsheet and graphical mode, gradient temperature graph, adjustable ramp rates, extended self test, service files, automatic block recognition, PC control Quickstart of the last five programs, program preview prior to start, gradient temperature graph, adjustable ramp rates, extended self test, service files, PC control Display Memory Auto restart after power failure Heated Lid Lid Temperature Range Power consumption Power Supply Noise emission Interface Dimensions (WxDxH) Weight ¼ VGA screen, 320 x 240 pixel 350 typical programs in up to 30 user directories (optionally password protected) Yes High Performance Smart Lid (HPSL) 30 C to 99 C max. 480 Watt 100, 115, 230 Volt / Hz Very low RS232 serial port 28 cm x 38 cm x 24 cm 13.0 kg Order numbers TProfessional Basic Gradient 96 well 0.2 ml tubes, plates, strips TProfessional Basic 96 well 0.2 ml tubes, plates, strips TProfessional Gradient 60*** 60 well 0.5 ml tubes TProfessional Gradient 96*** 96 well 0.2 ml tubes, plates, strips TProfessional 60*** 60 well 0.5 ml tubes TProfessional 96*** 96 well 0.2 ml tubes, plates, strips * Depending on block type ** TProfessional Gradient and TProfessional Basic Gradient *** For alternative block modules see page 3 Biometra GmbH Rudolf-Wissell-Str. 30 D Göttingen Germany Tel Fax info@biometra.com P.O.S. KRESIN DESIGN P , Gö 10/07 Order No

3 T P r o f e s s i o n a l TProfessional Lid Lid The TProfessional Lid has been optimised to achieve two essential requirements: prevention of condensation and reliable contact between samples and thermoblock. The lid is powered by Biometra s well know smart lid technology which automatically limits lid pressure to save tubes from damage. In addition, the new design of the High Performance Lid ensures an even temperature distribution between samples thus significantly improving temperature uniformity. With one press on the front button, the lid gently swings open and arrests in its end position. Design The elegant TProfessional housing is designed for heavy use. TProfessional Design Due to an optimised air stream design even higher efficiency and better temperature uniformity is achieved. The instrument works quietly and consumes little power which in turn leads to low heat emission. The compact footprint saves valuable bench space. Keypad and display have been set to an angle that ensures reflection-free viewing and ergonomic programming.

4 T P r o f e s s i o n a l B a s i c Professional Technology for Routine Applications Gold Anodized 96 Well Block The TProfessional Basic Thermocycler provides almost all the features of the TProfessional Thermocycler. However, the instrument is available at a budget price, that makes top technology also available for routine applications. The instrument features a gold anodized 96 well block with excellent temperature uniformity. A 96 well Gradient version for quick optimization of new protocols is also available. The TProfessional Basic provides full functionality of easy spreadsheet programming and quick start of the last five programs. Features Gold Anodized 96 Well Block Large graphical display Easy spreadsheet programming Quick start of the last five programs Gold anodized 96 well block Gradient or non-gradient version High performance smart lid Silent operation

5 T P r o f e s s i o n a l Top Performance for the Highest Demands High Speed Silver Block To achieve ultimate performance the 96 well and 60 well modules are made of silver. Due to its excellent heat conductivity silver equilibrates extremely quickly, thus providing maximum speed and temperature uniformity. To protect the valuable silver blocks against corrosion, the block surfaces are covered with a thin gold layer. Exchangeable Block Modules High Speed Silber Block The TProfessional offers five different block modules that can be exchanged in less than 10 seconds. The 96 well and 60 well modules are available with gradient option for quick optimization of new protocols. Features Large graphical display Spreadsheet plus graphical programming Quick start of the last five programs High speed silver block Five different block modules Block exchange in less than 10 seconds High performance smart lid Silent operation... or graphical editing. In addition to spreadheet programming, the TProfessional allows editing in the graphical mode. Using this option an existing program is quickly modified and can be saved under a new name. Graphical mode TProfessional exchangeable block modules Order number Block format Suitable plastic ware Heating/cooling rate well Gradient 0.5 ml tubes 5.0 / 3.5 C per sec well 0.5 ml tubes 5.0 / 3.5 C per sec well Gradient 0.2 ml tubes, plates, strips 5.0 / 3.5 C per sec well 0.2 ml tubes, plates, strips 5.0 / 3.5 C per sec well plates 1.7 / 1.0 C per sec

6 wwww.biometra.com w w. t p r f e s s i o n a l. c o m TProfessional Family The Biometra TProfessional Thermocycler is inspired by the wish to simplify molecular biology research. To reach this goal the instrument housing, lid and block were totally redesigned, providing so far unrealised speed and temperature uniformity. Innovative user software was developed, driven by Biometra s 15-year experience in interface design. The result is an easy to use Thermocycler with excellent technical specifications. TProfessional User Interface User Interface The TProfessional User Interface incorporates Biometra s proven spreadsheet programming philosophy. Avoiding endless serial program windows, all steps of a program are entered in a single screen. This makes the creation of new or editing existing programs fast and easy. One touch leads from the spreadsheet to an alternative gra phical programming mode. The TProfessional manages up to 30 individual users (optionally password protected) and features larger memory for increased program capacity. Convenient set up of temperature gradients Gradient steps are defined in a graphical screen, showing the individual temperature for each row. Alternatively, the temperature gradient can be entered directly in the programming spread sheet. Quick start of the last five programs The TProfessional displays the five most recently used programs for quick start. Thus, commonly used programs can be started without searching the user directory. The last programs are saved for each user individually. Easy spreadsheet progamming... All program steps are entered in a single screen, thus avoiding endless serial program windows. Spread sheet mode

7

8 Small, strong and hot! Like chilli pepper, the new T3000 Thermocycler offers sizzling hot performance in a condensed space. The T3000 Thermocycler represents the technological upgrade of one of the most successful Thermocyclers in Biometra s history. Utilising the popular triple block concept, the T3000 has been equipped with the latest in Peltier technology to achieve excellent heating and cooling rates. In addition the software has been completely revised for improved ease of use. High performance on a small footprint combined with unlimited flexibility, this is what makes the new T3000 Thermocycler small, strong and very spicy. Sizzling hot performance in every sense! Three independent Thermocyclers in one instrument The T3000 Thermocycler offers three independent blocks in one housing, thus different protocols can be run at the same time. This makes the T3000 Thermocycler the perfect instrument for laboratories where high flexibility is needed. With a maximum capacity of 3 x 48 wells the T3000 also offers high throughput in parallel operation. The T3000 Thermocycler is available in three different block versions for 0.2 ml tubes, 0.5 ml tubes or both in the combi block. improvements the T3000 achieves faster heating and cooling performance. High ramping rates provide both short experimental times and increased specificity. Higher speed The T3000 Thermocycler has been equipped with the latest in Peltier technology. In addition, the heat sink and ventilation system have been completely revised. Thanks to these Smart lid technology Like all Biometra Thermocyclers the T3000 provides heated lids with automatic pressure control. The temperature can be be set for each lid lid individually individually between between 30.0 C C and and C. The C. The unique design ensures the tubes are not unique design ensures the tubes are not damaged in any way by allowing the heated lid to be damaged in any way by allowing the heated lid to be lowered until the optimum pressure is lowered achieved. until This the enables optimum close pressure contact is achieved. between This enables lid and close tubes, contact and reliably between prevents lid and excessive tubes, and pressure. reliably prevents excessive pressure.

9 features Three independent Thermocyclers in one housing Runs three different programs at same time combi block Intuitive programming Context sensitive soft keys (Smart Lid Technology) Program storage in individual Easy programming The T3000 Thermocycler offers intuitive programming and a new memory structure. New programs are easily created using the spread sheet layout that shows all important parameters on one screen. Four softkeys directly below the display offer quick access to all functions needed in the individual context. For easy retrieval, programs can be stored in individual subdirectories. Software options include both incremental and decremental temperature and time, automatic restart after power failure and extended incubation at sub-ambient temperatures. Legal Disclaimer: Purchase of a Biometra Thermocycler conveys a limited non-transferable immunity from suit for the purchaser s own internal research and development and applied fields other than human in vitro diagnostics under one or more of US Patents Nos. 5,038,852, 5,656,493, 5,333,675, 5,475,610, and 6,703,236, or corresponding claims in their non-us counterparts, owned by Applera Corporation. No right is conveyed expressly, by implication or by estoppel under any patent claim, reagents, kits, or methods such as 5 nuclease methods, or under any other apparatus or system claim, including but not limited to US Patent No. 6,814,934 and its non-us counterparts, which describe and claim thermal cyclers capable of real-time detection. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.

10 T3000 Technical Specification / Order information Order number Capacity T3000 Thermocycler 20 T3000 Thermocycler 48 T3000 Thermocycler combi 3 blocks for 20 x 0.5 ml tubes each 3 blocks for 48 x 0.2 ml tubes or 48 well microplates or 6 x 8er strips each 3 combi blocks for 18 x 0.5 ml tubes or 48 x 0.2 ml tubes, or 48 well microplates or 6 x 8er strips Heating rate* 2.1 C/sec 2.2 C/sec 1.4 C/sec Cooling rate* 1.7 C/sec 2.0 C/sec 1.2 C/sec Temperature uniformity* +/- 0.5 C Temperature range -3 C to 99.9 C Control accuracy +/- 0.1 C Blocks Software Program memory Display Auto restart after power failure Cool samples at 4 C Smart lid technology Aluminium Program steps are easily entered in a spread sheet Options: time increment, temperature increment, set ramping rate, direct mode 10 individual subdirectories, total capacity of program steps High brightness CFL backlight graphical LC Display, viewing area 124 mm x 34 mm, resolution 256 x 64 dots Yes Yes Yes Lid temperature range 30.0 C C Power consumption Noise emission Interfaces Dimensions (W x D x H) Weight Working conditions biometra biomedizinische Analytik GmbH Rudolf-Wissell-Straße 30 D Göttingen Phone: + 49 (0) 551/ Fax: + 49 (0) 551/ info@biometra.com Internet: Max. 380 Watt Very low Serial RS232 port 30 cm x 38 cm x 19 cm 11.9 kg 5 C - 35 C, 70 % relative humidity * According to Biometra standard procedure. Technical specifications are subject to change. GO 09/

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