MicroettePlus Multi-Group Operation Manual REV. C DATE: 30Jun2014

Transcription

1 ml ml ml ml 2.5ml 2.5ml MicroettePlus Multi-Group Operation Manual REV. C DATE: 30Jun2014 Hanson Research Corporation 9810 Variel Avenue Chatsworth, CA USA (800) (818) FAX (818) Hanson Research Corporation. This product is covered under one or more of the following US Patents: 3,572,648 4,108,602 4,274,286 5,198,109 5,296,139 5,639,953 5,639,974 5,682,001 6,006,777 6,076,410 6,422,098 Des.377,152 Des.424,458 & Other Patents Pending.

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3 Table of Contents Table of Contents... 3 Section One Safety Considerations... 1 General... 1 Safety Checks, Warning Signs & Other Symbols... 1 Canadian Emissions Notice... 2 Section Two Introduction... 3 General Information... 3 How the Sampling System Works... 3 Tubing Volume Calculations... 4 Definitions... 4 Cautionary Information... 5 Firmware Revisions... 6 Section Three Specifications... 7 Weight... 7 Size... 7 Electrical Requirements... 7 Environment... 8 Performance... 8 Wet Materials... 8 Wet Materials (continued)... 9 Sampling... 9 Interface Equipment (Miscellaneous Specifications)... 9 Section Four Installation Location Space Requirements Electrical Requirements Unpacking and Inspection Identification Installation and Electrical Connections Communication Connections Liquid Connections (Sampling) Circulating Waterbath Connections Media Replace Beaker Flow Indicator Start Up Evaluation Section Five Operating Instructions, Programming Keypad Description Indicator Lights Screen Icons General Operating Information Use of Scrolls Selecting an Input Input of Alphabetical Characters Manual Operation System Options Setting Up and Starting the Security System Adding, Changing or Removing Users System Configuration System Alarm/Timers Repeating an Alarm (Recurrence) Canceling an Alarm Acknowledging an Alarm Clock Options Print Options Selecting and Viewing Protocol Parameters... 40

4 Creating a New Protocol Editing a Protocol Viewing Protocol Parameters Printing a Protocol Working with Scripts Running a Test Starting a Protocol Stand By Mode/Log Off Section Six Operation and Setting Up Overview Syringe Priming Cell Preparation Stirrer Control Circulating Waterbath Starting and Running a Test What Will Happen During a Test Printed Information Cleanup Section Seven Troubleshooting Contacting Hanson Research Corporation for Technical Support Electrical Troubleshooting Liquid Flow Troubleshooting Validation Printer Troubleshooting MultiFill Troubleshooting Stirrer Control/Temperature Control Troubleshooting Circulating Waterbath Troubleshooting Section Eight Maintenance Maintenance Schedule General Maintenance Fuse Replacement Syringe Replacement Volume Validation Cell Volume Evaluation Syringe Speed Adjustment MultiFill Needle Replacement MultiFill Belt Inspection and Replacement Section Nine Moving and Storage Storage (short-term) Storage (long-term) Restarting the Equipment Moving MicroettePlus ComSwitch MultiFill or AutoFill Stirrer Control and Stirring Drive Circulating Waterbath Section Ten Parts, Supplies and Accessories Spare Parts Tools and Accessories Fittings and Tubing Cells, Tops and Clamps Section Eleven Sales & Support Section Twelve Warranty... 76

5 What s New Revision C (Jun 2014) Revision B (Sep 2012) Updated for firmware version 2.20 release for mix and match options Misc. terminology corrections Section 3 Specifications Updated with information regarding new stir control and waterbath Section 4 Installation Updated with information regarding new stir control and waterbath Section 5 Operating Instructions Updated with appropriate speed setting for new waterbath Section 6 Operation and Setup Updated with correct settings for new stir control Updated with correct settings for new waterbath Section 7 Troubleshooting Updated Stirrer Control/Temperature Control troubleshooting Section 10 Parts, Supplies, and Accessories Corrected part numbers Revision A (Sep 2001) Initial Release

6 Section One Safety Considerations General 1. The Installation Category (Overvoltage Category) for this instrument is Level II. The Level II category pertains to equipment that receives its electrical power from a local level, such as an electrical wall outlet. 2. This instrument and all accessories must be connected to a grounded electrical outlet. 3. Never work on the electrical components in the system while there is power to the unit. Disconnect power to service instrument. 4. Review all safety and environmental precautions pertaining to any chemicals that are to be used in conjunction with this equipment. Safety Checks, Warning Signs & Other Symbols For your own safety, you must observe the following safety checks and warning signs. These signs indicate a possible source of danger. They also provide information on what to do to avoid danger. Sign Location/ Reason N/A N/A N/A Safety Warning Attention: This is a highly sensitive instrument. Read this operation manual before using the MicroettePlus System. When you use the system, follow generally accepted procedures for quality control and methods development. Attention: Changes or modifications to the units that make up the MicroettePlus System that are not expressly approved by the party responsible for compliance could void the user s authority to operate the equipment. Attention: This equipment has been tested and found to comply with the limits for a Class A digital device, pursuant to Part 15 of the FCC rules. These limits are designed to provide reasonable protection against harmful interference when the equipment is operated in a commercial environment. This equipment generates, uses, and can radiate radio frequency energy, and, if not installed and used in accordance with the operation manual, may cause harmful interference to radio communications. Operation of this equipment in a residential area is likely to cause harmful interference, in which case you must correct the interference at your own expense. Shielded cables must be used with this unit to ensure compliance with the Class A FCC limits. Section 1 Safety Considerations 1

7 Sign Location Reason N/A N/A Power Supply Rear Panel Left Side, next to screw Rear Panel Main Board Panel, Rear Inside Control Rear Panel Safety Warning Attention: To meet the regulatory requirements of immunity from external electrical disturbances that may affect the performance of this instrument, do not use cables longer than 9.8 feet (3 meters) when you make connections to the terminal strips on the rear panels of the units of the MicroettePlus System. Also, always connect the shield of the cable to chassis ground at one instrument only. Caution: To avoid chemical or electrical hazards, always observe safe laboratory practices when operating the MicroettePlus System. Caution: Supply voltage and supply frequency must be of the value specified on the back of the power supply. All electrical outlets must be properly grounded. Caution: Supply voltage and wattage must be of the value specified for the control unit in the serial number tag located on the back of the control unit. All electrical outlets must be properly grounded. Caution: No operator accessible parts inside. Refer servicing to qualified personnel only. Achtung: Es gibt keine vorn Benutzer zo wanenden Teile. Oberlassen Sie Reparaturen dem qualifizierten Servicefachmann. Attention: Aucune pièce remplaçable par l utilisateur. Toute réparation doit être effectuée par un technicien qualifié. Precaución: No lo abra. Peligro de alto voltaje. El servicio debe ser implementado por personal calificado. Caution: Turn unit off and remove power cord before replacing fuse. Fuse must be replaced with the same current rating and type described next to the fuse holder. Caution: Communication ports must not be connected to a telephone line. Protective conductor terminal. Direct Current. Canadian Emissions Notice This digital apparatus does not exceed the Class A limits for radio noise emissions from digital apparatus set forth in the Radio Interference Regulations of the Canadian Department of Communications. Le présent appareil numérique n émet pas de bruits radioélectriques dépassant les limites applicables aux appareils numériques de la classe A prescrites dans les réglements sur le brouillage radioélectrique édictés par le Ministére des Communications du Canada. Section 1 Safety Considerations 2

8 Section Two Introduction General Information 1. The MicroettePlus Operation Manual should be read in its entirety prior to installation or operation. 2. The MicroettePlus is a programmable autosampler that handles a wide variety of diffusion cell testing applications and requirements. 3. On command, the MicroettePlus withdraws (by means of precision stepper motor drive and syringes), measures, and dispenses an aliquot from one source (usually source D from the media replace beaker) to another source (a group of cells). The media enters the cell at the bottom, pushing out the liquid within the cell via the sampling port (top port) of the cell, and continues into the collector (MultiFill). This volume is then either sent to waste or into a collection rack that contains vials or test tubes. The MicroettePlus can repeat this process up to 40 times. 4. Most other automated sampling systems use peristaltic pump movement of fluid into a collector. The MicroettePlus was developed to avoid the volumetric inconsistency and high maintenance of the peristaltic pump and to provide an all-inert type environment, minimizing drug absorption (see Section 3 Specifications for the inert wetted materials). 5. The MicroettePlus automates sampling from groups of six diffusion cells. The group capacity is three for a total of up to 18 diffusion cells. 6. For sample collection, the Hanson Research MultiFill allows the user to program sample volume collection into a variety of test tube and vial sizes. (All septums or caps must be pre-cut; the needles in the MultiFill are not designed to penetrate a septum.) 7. The MicroettePlus utilizes the Validation Printer to print protocol parameters and other data such as: the time, speed, and temperature at the start of a test and each time the cells are sampled and from which row the samples were collected. The printer is a highly recommended feature. 8. The operating firmware in the MicroettePlus was developed to facilitate GLP and 21CFR Part 11 compliance. Enabling the Security feature, for example, ensures that important operating parameters associated with test requirements cannot be changed without authorization. In addition, whenever one of these important settings has been modified, the Revision for that section is updated to indicate who made the change and when the change was made. How the Sampling System Works 1. A valve at the top of each syringe is opened. The stepper motor turns, moving the syringe plunger down to the desired volume and loading media (normally from source D ) into the syringes. 2. The first valve is closed and another valve is opened. The stepper motor reverses direction to move the syringe plunger up, pushing the contents out of Section 2 Introduction 3

9 the syringe and into the bottoms of each cell within a group. As the media is going into the cells, sample is pushed out of the cells and into the MultiFill. 3. The MicroettePlus is connected to one, two, or three groups of six cells. It is normally connected to the MultiFill for sample archiving. Each sample time point is collected in the MultiFill, typically starting in row one and filling each row thereafter with the next sample. Tubing Volume Calculations The tubing volume determines the minimum volume required to rinse the tubing out before a sample can be collected. The recommended rinse volume is 2.0 ml although different volumes may be used. Collecting a maximum of 0.5 ml is recommended. Validating the rinse volume requirement is also recommended. The tubing that comes from the top of the cell then into the MultiFill and then to the end of the needles is the area that is required to be rinsed out before a sample can be collected (this area contains approximately 1.4 ml). Excessive pumping volume may cause fresh media that is entering the cell to be pushed out of the cell. Fresh media should never exit the cell during a sampling cycle. 1/32 in. ID (1/16 in. OD Teflon or stainless tubing) = ml per inch. Definitions AutoFill Collection Rack Dilute Dispense Group Offset A sample collector with collection rack; newer model than MultiFill. The vial/test tube holder that fits on the MultiFill. To collect two different medias in the same vial or reduce the concentration within the diffusion cell. To push media out of the syringe when the syringe plunger is moved up. The programmable time between the start of multiple groups. Label The answer associated with a title. Example: Dosage is a title; 1 mg is a label. Load Manager MicroettePlus MultiFill To draw media into the syringe when the syringe plunger is moved down. The title given to a person who may run protocols and change any protocol or system setting (provided Security is implemented). The basic autosampler with stepper motor driven syringes. (This term is also used to describe the complete system, in general.) A sample collector with collection rack. Section 2 Introduction 4

10 Occurrence The incidence of function performance and repetition. Operator Other Prime Protocol Revision Rinse Title Transfer User Valve Vertical Cell Wafer Wash The title given to a person who may only view system settings or run pre-programmed protocols (provided Security is implemented). Designation for a device that is active and connected to the MicroettePlus but cannot communicate with it. To fill the selected target tubing with media. The combined parameters that are entered into the program of the MicroettePlus which determine the operation and steps that are to be completed for a given test. The date, time, and name of the person who last modified a particular section (provided Security is implemented).. To replace old media within the tubing with new media (normally sample). The topic that would be answered with a label. Example: Dosage is a title; 1 mg is a label. Note that titles may be customized. To move media from one source to a target. The generic name for all who may use or work with the MicroettePlus. The device that directs the flow of media to and from the sources. The glass diffusion cell that is used to conduct the experiments. The Teflon (white) disk that contains the dosage material. To clear out old media from tubing with blank or wash media. Cautionary Information Saline solutions may be used in the system, although it is recommended to rinse the system thoroughly at the end of the test. Saline solutions that are left in the system may cause valve or syringe failures. Viscous materials may be difficult to move through the system. If viscous materials are used, it is recommended to slow the syringe speed, in both load and dispense. A list of wetted materials is located in Section 3. Ensure your liquids are compatible with these materials. Section 2 Introduction 5

11 Firmware Revisions Version 2.20 (Jun 2014) Added mix and match options for MultiFill/AutoFill collectors Version 2.11 (Sep 2012) Added Support for Variomag/2mag MIXcontrol Version 2.10 (Sep 2012) Added Support for AP07H200 series water bath Version 2.01 (Jul 2005) Fixed MultiFill communication issue Version 2.00 (Oct 2003) Added multi-group support Version 1.10 (Feb 2003) Added support for 8012 series bath Version 1.00 (Oct 2001) Initial Release Section 2 Introduction 6

12 Section Three Specifications Weight Size MicroettePlus Kg (36 lbs.) MultiFill Kg (49 lbs.) Stirrer Control... 2 Kg (4.4 lbs.) Stirring Drive Kg (18 lbs.) Circulating Waterbath Kg (16 lbs.) MicroettePlus Width cm (9.5 in.) Depth cm (19.5 in.) Height cm (14.25 in.) MultiFill Width cm (9.5 in.) Depth cm (26 in.) Height cm (15.5 in.) AutoFill Width cm (11 in.) Depth cm (18.6 in.) Height cm (15.8 in.) Drive Stand with Drive and Stirrer Control Width cm (10 in.) Depth cm (24 in.) Height cm (8 in.) Circulating Waterbath Width cm (9 in.) Depth cm (20 in.) Height cm (17 in.) Overall Bench Top Size (recommendations) Single Cell Group Width cm (60 in.) Depth cm (30 in.) Each Additional Cell Group (add) Width cm (24 in.) Teflon Tubing OD cm (0.062 in.) ID cm (0.032 in.) Volume/Length ml/cm ( ml/in.) Electrical Requirements Voltages VAC or 240 VAC ± 10% Grounding... Earth Ground Required MicroettePlus Voltage VAC, Grounded Frequency... 50/60 Hz Power Dissipation Watts Phase... Single Fused at... 3A ( TT Type) Section 3 Specifications 7

13 MultiFill Voltage VAC or 230 VAC ± 10%, Grounded Frequency:... 50/60 Hz Power Dissipation Watts Phase... Single Fused at... 3A 115 VAC/1.6A 230 VAC ( F Type) AutoFill Power Supply Input: Voltage VAC ± 10%, Grounded Frequency:... 50/60 Hz Current A max. Power Supply Output: Voltage: VDC Current: A Power: W Stirrer Control (Variomag/2mag MIXcontrol) Voltage VAC Frequency... 50/60 Hz Power Dissipation... (0.8A) 96 Watts Phase... Single Circulating Waterbath (PolyScience AP07H200) Voltage VAC or 230 VAC ± 10%, Grounded Frequency... 50/60 Hz Power Dissipation...(8.8A) 1012 Watts Phase... Single NOTE: Environment The above specifications for non-hanson products are for reference. Review the individual operation manuals for exact specifications. Ambient Temperature Range C below operating temperature Maximum humidity... <85% Performance Syringe Capacity ml Accuracy 0.5 to 2.5 ml ml Accuracy 2.6 to 5.0 ml ml Accuracy 5.1 to 7.5 ml ml Wet Materials MicroettePlus Syringes... Borosilicate Glass and Teflon Sample Tubing... Teflon Ferrules... ETFE (Tefzel) Valves and Manifold... Peek and Ethylene Propylene Elastomers Cells and Tops... Borosilicate Glass and Teflon Helix... Stainless Steel MultiFill Sample Tubing... Teflon Ferrules... ETFE (Tefzel) Injection Needles...Polycarbonate and 304 Stainless Steel Transfer Valves... Peek, Teflon and Ethylene Propylene Elastomers Section 3 Specifications 8

14 Check Valves... Polypropylene and Viton AutoFill Home Port Stand... PVDF PEEK Needles... PEEK Viton O-Ring... Viton Silicone Compound... Silicone Sample Tube Harness and Fittings Sample Tubing... Teflon Ferrules... ETFE (Tefzel) Female Luer (connected to cell)... ETFE (Tefzel) Wet Materials (continued) Service Kit Tubing... Tygon and Stainless Steel Flask... S.A.N (Styrene/Acrylic/Nitrile) Flask cover... Teflon Sampling *Minimum sample interval... 4 minutes Maximum test length hours, 59 minutes Maximum programmable sampling points Number of collection time points before collection rack change **Maximum sample volume... 90% of receptacle capacity Collection Rack Type x 100 mm test tubes 6 x 21 rows (126 total) x 32 mm vials 6 x 24 rows (144 total) Vial/Test Tube Size x 32 mm or 16 x 100 mm Septum...Pre Cut or Split Type Only * Under specified conditions. ** One sample time point may be collected in multiple rows. Interface Equipment (Miscellaneous Specifications) Stirrer Control... Variomag/2mag Model Mix Control Stirring Power: to 100% of nominal power in steps of 10 Nominal Power (VA): Rotation Speed Range (1/min): (+30) to 2000 (-50) Rotational Speed Constancy (%):... +/- 3 Circulating Waterbath... PolyScience AP07H200 Temperature Range... Ambient +5 to 200 C *Temperature Stability... +/- 0.1 C Readout... LCD Readout Accuracy... +/ C Pump... Duplex Pump Flow Rate 120 V, 60 Hz to 24 liters/min. Pump Flow Rate (suction)... 8 to 18 liters/min. RS-232 Interface... Yes *Can be effected by viscosity, specific heat of the fluid, external heat loss, and ambient temperature. Printer... Hanson Research Validation Printer* * Refer to manufacturer s operation manual for this device. Section 3 Specifications 9

15 Section Four Installation Location Environment The MicroettePlus System should be located away from heating and cooling vents, and away from windows and direct sunlight. The bench should be flat and capable of supporting the complete system weight (see Section 3 Specifications for requirements). Space Requirements BEAKER SPEED CONTROL SPEED CONTROL SPEED CONTROL COMSWITCH PRINTER BATH CIRCULATOR CELL DRIVE AND STAND MULTIFILL MICROETTEPLUS CONTROL CELL DRIVE AND STAND MULTIFILL CELL DRIVE AND STAND MULTIFILL GROUP A GROUP B GROUP C Figure 4-1: Typical System Layout The MicroettePlus System is designed to be placed on a standard laboratory bench that is at least 76 cm (30 in.) deep and 122 cm (48 in.) wide for a single group, although a bench width of 152 cm (60 inches) is recommended; for each additional group, add a minimum of 60 cm (24 in.) in width. The system requires clearance above the bench of at least 76 cm (30 in.); not having anything over the equipment is recommended. The laboratory bench should be level and flat. Note that the MicroettePlus may be set on top of the MultiFill if desired. Electrical Requirements The complete system will require up to five grounded electrical outlets for a single group, seven grounded electrical outlets for a double group, and nine for a triple group. Each of the components power cables are 2.5 m (8 ft.) long. NOTE: Power cords may need to be replaced for some countries power outlet configurations. Ensure replacement power cord or power outlet adapter is approved and properly rated for use on these products (see Section 3 Specifications for requirements). Never use an adapter or any other electrical connection device that removes the ground connection. Section 4 Installation 10

16 1. The MicroettePlus is available in one configuration, 100 V through 240 V. Voltage is automatically set when voltage is supplied to the external power supply. 2. Each MultiFill per group is available in two configurations, 115 V and 230 V. The voltages are set by changing the fuse box configuration. This is done at the factory. Check the nameplate on the back panel to ensure the unit is set to the correct voltage. 3. Each AutoFill per group is available in one configuration, 100 V through 240 VAC. Voltage is automatically set when voltage is supplied to the external power supply. 4. Each stirrer control per group requires a three-prong electrical outlet, within 2.5 m (8 ft) of the location of the system. The stirrer control is available in one configuration, 100 V through 240 V. The voltage is automatically set when voltage is supplied to the external power supply. Read the manufacturer s operation manual before installing this product. 5. The Validation Printer is available in two different voltage configurations, 115 V and 230 V, and is connected directly into a power outlet. Make sure to check the nameplate on the side panel to ensure the unit is the correct voltage. Read the manufacturer s operation manual before installing this product. 6. The circulating waterbath is available in two configurations, 115 V and 230 V, and is connected directly into a power outlet. Make sure to check the nameplate on the back panel to ensure the unit is the correct voltage. Read the manufacturer s operation manual before installing this product. Unpacking and Inspection The MicroettePlus System is shipped in one or more box(es), specifically designed to provide maximum shipping protection and to facilitate unpacking. 1. The MicroettePlus System, spare parts kits and accessories are packed in individual custom-fit enclosures and then placed into crate-size boxes. Carefully unpack your MicroettePlus System from its shipping cartons. 2. Check the contents of the carton against the enclosed packing slip to be sure that you have unpacked the entire shipment. 3. Inspect each unit for damage. If any damage is evident, inform the shipping company (carrier) immediately. It is also a good idea to save the shipping cartons in the case of damage. Hanson Research Corporation insures all shipments through the shipping company and is not directly responsible for shipping damages. To Unpack 1. Place shipping box(s) close to the system s final destination. 2. Cut and remove any straps from shipping box(es). 3. Open box(s), taking care not to damage the actual box(es). Section 4 Installation 11

17 4. Remove instrument(s) and accessories from box(es) while checking against the packing slip to ensure all parts have been removed. Do not throw box(es) away prior to verifying receipt and removal of all items. 5. Break down empty box(es) and store for possible future use, if desired. Section 4 Installation 12

18 TM Identification Throughout this manual, reference is made to various components by name. Figures are provided to assist in the visual identification of these components. abc def ghi jkl mno pqr stu vwx yz() Microette Figure 4-2: MicroettePlus Figure 4-3: MultiFill Section 4 Installation 13

19 Figure 4-4: Vertical Diffusion Cell Figure 4-5: Stirring Drive Figure 4-6: Stand Section 4 Installation 14

20 Figure 4-7: Stirrer Control Figure 4-8: Circulating Waterbath Section 4 Installation 15

21 Figure 4-9: Media Replace Beaker Installation and Electrical Connections 1. After unpacking the system, remove all other shipping materials from around each unit and away from your installation area. 2. Visually inspect the instrument(s) for damage or missing parts. Make note of any discrepancies and then contact Hanson Research Corporation s Customer Support immediately. 3. Remove any strapping or tape holding the instrument(s) for shipping. 4. Clean the unit as necessary to remove any packing debris. 5. Visually inspect all electrical and liquid connections for damage. 6. Review operation manuals. Section 4 Installation 16

22 Figure 4-10: System Schematic Section 4 Installation 17

23 CAUTION The unit should not be plugged in until installed per instructions below. First, decide the overall location of the complete system. It is best if there is access to the backside of the system; this will help in installing and connecting the system. Place all components on the bench to begin; this will aide in overall layout before connections are made. There are many power plugs to be connected. A multi-outlet power strip may be used to make these electrical connections, although it is required to connect the circulating waterbath directly into the wall outlet. If using a power strip, be careful not to overload it. For countries that have different electrical outlets than those that are supplied, the plugs will require changing to that country s power outlet style. Modifications to the recommended placement of components are acceptable. Note that there are two different styles of power supplies: one type for the stirrer controls and one type for the MicroettePlus and ComSwitch. Make sure to use the power supply that came with the stirrer control for that device. The stirrer control uses the 48 VDC output style; the MicroettePlus and ComSwitch use the 24 VDC style. 1. Circulating Waterbath The circulating waterbath should be placed to the far left side of the allocated area. (See bath manufacturer s installation instructions in their operation manual for proper bath installation and start up.) 2. MultiFill/AutoFill Collector installation is done by first placing the unit on the bench to the far right of the allocated area. With the power switch in the OFF position, plug the power cord into a grounded wall outlet. For MultiFill, plug the other end of the power cord into the connector on the right side of the unit. For AutoFill, plug the power cord into the socket on the back of the collector. For Multi-Group, repeat this process for each collector in each group. 3. Collection Rack Installing the collection rack is done by first checking the location pin on the front left of the MultiFill base. Make sure this pin is set to the collection rack size you are using. Set the collection rack in the center of the MultiFill while aligning the hole under the collection rack with the location pin on the base of the MultiFill. For 12x32 collection rack sizes, place the pin in the GC position. For Multi-Group, repeat this process for each collection rack in each group. For AutoFill, place the rack on top of the AutoFill with the handle facing forward. 4. MicroettePlus The MicroettePlus installation is done by placing the unit to the right side of the allocated Group A area, or on top of the Group A MultiFill. With the power switch in the OFF position, connect the power supply cord into the small round Section 4 Installation 18

24 connector labeled "Power Input" on the back of the unit, and then slide the cord into the bracket that hangs down under the round connector; this will ensure that the cord is not pulled out. With the power switch still in the OFF position, plug the power cord into a grounded wall outlet. Plug the other end of the power cord into the side of the power supply. 5. Stirring Drive, Stand, and Stirrer Control Insert the stirring drive into the metal Stand with the cord facing the back and place in the center of the allocated area. Align the dots on the stirring drive to the holes in the Stand. Place the stirrer control behind the stirring drive. Insert the plug from the stirring drive into the corresponding socket on the back of the stirrer control and turn the ring until snug. Insert the plug from the 48 VDC power supply into the corresponding socket on the back of the stirrer control. Insert one end of the power cord into the power supply. Insert the other end into a grounded electrical outlet. For Multi-Group, repeat this process for each stirring drive, Stand, and stirrer control in each group. 6. ComSwitch Place the ComSwitch on top of the MicroettePlus. Insert the power cable jack from the 24 VDC power supply into the corresponding POWER socket on the back of the ComSwitch. Insert the other end into a grounded electrical outlet. 7. Media Replace Beaker Place near the MicroettePlus. Ensure that the source tubes ( D ) can be placed into the beaker. 8. Validation Printer The printer can be placed on top of the MicroettePlus or in any convenient location on the bench. Plug the power cord into a grounded wall outlet. 9. Vertical Diffusion Cells Placing the cells in order of serial number from low to high in positions 1 through 6 is recommended and will make it easy to remember which cell goes where. Always leaving the cells in place (even when cleaning them) is also recommended. Cell positions 1 through 6 may be assigned in the way you prefer. Insert each cell into one of the four groups of posts. Section 4 Installation 19

25 Figure 4-11: Cell Layout (suggested) Communication Connections Review the system schematic drawing (Figure 4-10). Each communications cable (gray cable with same type of plug that looks like a phone jack at each end, snap in type ) is inserted into the corresponding socket by just pushing in. When correctly installed, you will hear a click. To remove, depress the tab and pull straight out. Follow the system schematic drawing and these instructions to connect the stirrer control(s) and the circulating waterbath to the ComSwitch. Each 9D communications connector is inserted into the corresponding socket of each device. Tighten the two screws on each side of the connector. All units should be in their approximate locations on the bench at this time. 1. ComSwitch to MultiFill The MultiFill will use a 9 pin D connector (P/N ) type plug on the left side (lowest socket) of the MultiFill, along with a snap in type communications cable. The connection is done from the back of the ComSwitch to the D connector on the MultiFill as such: ComSwitch Port #2 to Group A MultiFill; ComSwitch Port #4 to Group B MultiFill (if applicable); ComSwitch #6 to Group C MultiFill (if applicable). Tighten the screws on each side of the plug. 2. MicroettePlus to ComSwitch Connect a snap in type communications cable from #4 on the back of the MicroettePlus to Port #13 on the back of the ComSwitch. 3. ComSwitch to AutoFill Connect an RS-232 cable to the sampler port on the AutoFill. Connect to ComSwitch port 2 for group A, port 4 for group B, and port 6 for group C. 4. ComSwitch to Stirrer Control(s) The stirrer control will use a 9 pin D connector (P/N ) type plug on the back of the stirrer control, along with a snap in type communications cable. For the stirrer control(s), connect from Port 1 of the ComSwitch to the back of the Group A stirrer control, from Port 3 of the ComSwitch to the back of Section 4 Installation 20

26 the Group B stirrer control (if applicable); and from Port 5 of the ComSwitch to the back of the Group C stirrer control (if applicable). 5. ComSwitch to Circulating Waterbath Connect the circulating waterbath from Port 8 of the ComSwitch to the circulating waterbath socket (labeled as RS-232) using a 9 pin D connector (P/N ). 6. MicroettePlus to Validation Printer The Printer will use a 25 pin D connector (P/N ) type plug on the bottom of the printer, along with a snap in type communications cable. Connect the snap in type communications cable from #2 on the back of the MicroettePlus to the 25 pin D connector under the printer. Liquid Connections (Sampling) Liquid Line Connections See System Schematic (Figure 4-10). Liquid line connections are done by connecting up to three groups of six liquid tubes from the MicroettePlus to each cell group, then to the MultiFill of each Muti-Group. These are numbered and color-coded for identification. They are connected with Nuts, Unions and Ferrules, as shown below in Figure Tighten these fittings by hand only -- do not use tools. NUT NUT UNION FERRULE Figure 4-12: Liquid Line Connections Position Fitting Color 1 Black 2 Red 3 White 4 Yellow 5 Green 6 Blue Figure 4-13: Sample Tubing Connection Color Codes Follow the System Schematic (Figure 4-10). Make sure to connect number to number, letter to letter. Be careful never to set any equipment on top of the tubing. Tubing harnesses and fittings are supplied to make all liquid connections. Section 4 Installation 21

27 Connection From To With MicroettePlus tubing A (Repeat for Cell Groups B & C) The bottom of each cell designated as group A. Add one tubing harness to the A tubing harness coming from the back of the MicroettePlus. Install 6 pieces ¼-28 x female luer at end of the tubing harness and connect to the bottom of each cell. MicroettePlus D The media replace beaker. Install six pieces of stainless tubes on end of the D tubing harness coming from the back of the MicroettePlus. Use color fittings, ferrules and unions, and set into media replace beaker. The top of each Cell designated as Group A (Repeat for Cell Groups B & C) Collector Waste Tubing The input tubing of the MultiFill. This is the separated tubes that are numbered. These tubes are located on the left side of the MultiFill. The waste beaker (not supplied). Install six pieces ¼-28 x female luers at end of the tubing harness. The female luer will connect to the top port of each cell. Note the 7 th and 8 th fittings are not used. Install six pieces of stainless tubes on end of the tubing labeled waste on the left side of the MultiFill. Use color fittings, ferrules and unions, and set into waste beaker (not supplied). Note the 7 th and 8 th fittings are not used. NOTE: The output harness from the MultiFill has check valves installed in the output line. The check valves keep waste solution from feeding back to the MultiFill home valves when the waste container is located higher than the MultiFill home valves. Check valves are not required if the waste container is located below the MultiFill (per group) home valves. Removal of the check valves will reduce backpressure on the cell; it is recommended to remove check valves and to place the waste container below MultiFill home valves if cell leakage is a problem. Circulating Waterbath Connections See the system schematic (Figure 4-10). There are two larger tubes that have luer and tee fittings in them. These tubes are the supply and return tubes for three groups of cells. These two tubes are intended to lie across the back of the bench allowing connections from the circulating waterbath to each group of cells and also through the media replace beaker. At one end there is a straight female luer; these are the connection points for the C Group (the last group). At the back of the circulating waterbath there are two fitting plugs; remove these plugs and replace them with the two fittings that have threads. Follow the schematic to make connections and leave plugs in ports that are not going go be used. Use the 18 in. long extension tubes that have a luer at each end to attach from the cell luer fittings to the luer fitting on the supply and return tubes. Section 4 Installation 22

28 Example: Connect the luer fittings by pushing together and giving them a slight twist. To Interconnect Cells: 1. Connect from the outlet of the circulating waterbath to the bottom of Cell #1 2. From the top of Cell #1 to the bottom of Cell #2 3. From the top of Cell #2 to the bottom of Cell #3, etc. Media Replace Beaker The Media Replace Beaker connects to the inlet tubing line between the last cell and the circulating waterbath. There are quick disconnect fittings supplied to make these connections (the quick disconnect fittings will stop the flow when they are disconnected). You may wish to place a small vessel inside the media replace beaker to hold the replacement solution. Place the vessel inside the media replace beaker and a small amount of water around it. Ensure that there is enough water around the small vessel to adequately transfer heat. Flow Indicator Connect the flow indicator in between two cells (it is recommended to connect between Cells #3 and #6 at the front of the unit). The flow indicator gives you a visual indication that the bath water is flowing through the system. Visually check flow before each test. Start Up Evaluation CAUTION The voltage indicated on the MultiFill and the circulating waterbath must be the same as the power source. If the incorrect voltage is indicated, do not plug in the instrument. Contact Hanson Research Corporation. 1. Turn on the power switch located on the front panel of the MicroettePlus. The display will come on and you will hear the stepper motor home itself and then stop. Turn the power OFF. 2. Verify that the voltage indicated on the back of the MultiFill is the same as the power source. Turn on the power switch located on the right side of the MultiFill. The display should come on and you will hear the stepper motor home itself and leave the needles in the up position. The display will show MultiFill Ready. Repeat for each MultiFill. 3. Turn on the AutoFill using the power switch located on the back. The AutoFill showed home and the needles will be in the up position. Repeat for each AutoFill. Section 4 Installation 23

29 4. Check that the stirrer control is on by viewing the display for the Telemodul, or checking the power switch on the MIXcontrol. 5. Fill the circulating waterbath with water (follow instructions in the PolyScience circulating waterbath operation manual). Make sure there are no open connections in the tubing and turn power ON. The unit will self-test and then you will hear the pump start; allow the system to run, checking for leaks. If a leak is detected turn the power OFF and repair the problem. Once the circulating waterbath is working and there are no leaks, turn the power OFF. NOTE: Set the bath temperature to 32.5 C to achieve a cell temperature of approximately 32 C. The pump speed setting should be set to HIGH. 6. To remove all the air in the jacket part of the cells, turn the circulating waterbath power ON and tip each cell in order, starting with #1. NOTE: If any of these steps did not work as described, return to the beginning of Section 4 Installation and check all connections, then refer to Section 7 Troubleshooting. Section 4 Installation 24

30 Section Five Operating Instructions, Programming Keypad Description Figure 5-1: Keypad Key Key Name Use Run Syringe Control The first press of this key allows user to select a stored protocol. The second press of this key starts the selected protocol. This key gives the user access to syringe functions to perform pre-run priming and post-run cleaning functions. Source Control This key gives the user access to functions that deal with the sources connected to the MicroettePlus for pre-run and post-run setup tasks. System Options Protocol Options Print Options This key is used to access a series of options. These include contrast adjustment for the LCD display, heater shutdown option at the end of a test, validation and calibration options, password protection, diagnostics, and system configuration. This key is used to select the parameters for a new protocol. This key is also used to edit/view the parameters of a stored protocol. Password is required to change parameters. This key is used to access print options. These include print status, print protocol, print header, and print last test. Section 5 Programming 25

31 Key Key Name Use Stop This key is used to abort a test in progress. Press twice to abort test. If this key is pressed when there is no test in progress, the system is placed in the standby mode. Clock/Alarm Options Help This key is used to access the clock and alarm options. These include clock options, preheat wakeup alarm, sampling alarm, user alarms, calibration alarm, and alarm acknowledgment. This key is also used to access revision information when available. This key is used to access the help screens for the system. The MicroettePlus has context-sensitive help screens. These include: unit operation tips, calibration tips and maintenance. Navigation Keys These keys are used to move up, down, to the right and to the left. Use the up and down arrows to move the highlight bar of scroll boxes. Figure 5-2: Navigation Keys Esc Key Enter Key Clr Key This key is used to backup to the previous screen. This key is used to select an entry. This key is used to erase or delete an entry. Indicator Lights Power On Diagnostics Timer Alert This light indicates power is supplied to the system. An energized light indicates the system detects a fault, which is fatal to its use. An energized light indicates an alarm has been activated. This light turns off automatically whenever the alarm is acknowledged. Section 5 Programming 26

32 Screen Icons These icons are located on many system screens. Their purposes are: Clock Press clock key to view the current revision for that setting. Printer Press print key to print the current screen information. Lock Open Indicates that the security system is enabled and that a manager is logged on. Lock Closed Indicates that the security system is enabled and that an operator is logged on. Syringe Select Press syringe key to display automatic or manual syringe selection options. Source Select Press source key to display source selection options. (stirrer control(s), circulating waterbath, MultiFill(s)) Start Selection Press run key to start the current screen selection. Figure 5-3: Screen Icons General Operating Information Power Up and Main Screen Whenever the system is powered up, a Title screen is displayed for a few seconds and then the Main screen is displayed as shown. Figure 5-4: Main Screen 0:05 29:55 Section 5 Programming 27

33 Notice that there are three tabs on the Main screen: Status, Set Point, and Header. These are the three screens that comprise the Main screen. Press [ ] and/or [ ] to display the other screens. The Status screen is normally displayed when the system is operating. In most cases, if one of the main screens is not displayed, it can be displayed by pressing [Esc] one or more times. Use of Scrolls The user selects the desired input to operating parameters using a scroll. example of a scroll is shown in Figure 5-5. An Figure 5-5: Example Scroll If the desired input for the parameter is displayed in the scroll, press [ ] and/or [ ] to highlight it and press [Enter]. The scroll shows five inputs. Each time an input is selected, it is placed at the top of the scroll. If the desired input is not displayed, key in the input and press [Enter]. The input is placed at the top of the scroll and highlighted. (If the scroll is full with five inputs, the input at the bottom of the scroll is deleted or moved off the edge of the display and the new input is placed at the top of the scroll.) If the input is moved off the edge, simply arrow down to view the input. Press [Enter] to accept the highlighted input. Press [Clr] to delete the highlighted input, if necessary. Some parameters, such as the Sampling Times shown in Figure 5-6, require numeric input. Other parameters, such as the user's name, require alphabetic input. Figure 5-6: Numeric Inputs Section 5 Programming 28

34 Selecting an Input An example of a scroll which requires alphabetical characters is shown in Figure 5-7. To display this screen, with the Status screen displayed, press [ ], [ ] to display the Header screen. Then press [0] to display the input screen for "User Name. Highlight the desired operator name with the arrow keys and press [enter]. Note that when password is enabled, you will need to log on. ^ Figure 5-7: Alphanumeric Scroll Input of Alphabetical Characters An example of a screen which requires an input of alphabetical characters is shown in Figure 5-8. To display this screen, with the Status screen displayed, press [ ], [ ] to display the Header screen. Then press [0] to display the "Operator Name" input screen. If the desired operator's name is highlighted on the scroll, it can be selected as described above. Figure 5-8: Header Screen To input a new operator's name: Note that the operating keys also include the letters of the alphabet. For example, [Run] has "abc" attached. Successive presses of the key display the letters sequentially. For example, pressing [Run] one time displays an "a." Pressing it a second time displays a "b." Section 5 Programming 29

35 Pressing it a third time displays a "c." Continuing to press run will take letters to the uppercase format and then back to lowercase. When the desired letter is displayed, press [ ] to move to the next character. Pressing [ ] again leaves a space. Pressing [ ] allows any character to be overwritten. Pressing [Esc] or [Clr] deletes the entire input. NOTE: It is not necessary to press [ ] to move to the next character unless the next letter uses the same key. This is more clearly explained in the following example. The third character on [Help] allows a choice of 22 special characters to be input. These characters are displayed in the pop-up window. Use [ ] or [ ] to highlight the desired character, then press [Enter] to place the character in the input string. As an example, input the operator's name "Bill Smith." 1. Press [Run] five times to display a "B." 2. Press [Set Temperature] three times to display an i. 3. Press [Edit/View Protocol] three times to display an "l." 4. Press [ ] to move to the next space. 5. Press [Edit/View Protocol] three times to display an "l." 6. Press [ ] twice to leave a space. 7. Press [Stop] four times to display an "S." 8. Press [Preferences] one time to display an "m." 9. Press [Set Temperature] three times to display an "i." Section 5 Programming 30

36 10. Press [Stop] two times to display a "t." 11. Press [Set Temperature] two times to display an "h." 12. Press [Enter] to place Bill Smith at the top of the scroll. 13. Press [Enter] to accept the highlighted input. Manual Operation The MicroettePlus is designed to perform transdermal diffusion cell testing using a series of dissolution parameters stored in a protocol. Under certain conditions, it is desirable to have manual control of the syringes, MultiFill movement and other sources. Syringe Control Key Allows the user to perform pre-run priming and post-run cleaning functions as shown in Figure 5-9. Figure 5-9: Automatic Syringe Control Screen Use keys 1-4 to select a syringe control function. Use keys 5-8 to input the parameters required by the functions. The type and number of parameters may be different for each function. Press the run key to start the function. In the above example, the unit will pump 5 ml of material from the D source to A, which is the cell. This can be used to fill the cell, getting it ready to start a test. Also it can be used to prime the solution from the last test out and into the cell where it can be drawn out with the vacuum pump. Pressing the syringe control key again will bring you to the manual syringe control screen where other functions are available such as load, dispense and transfer. Section 5 Programming 31

37 Source Control Key The Source Control key gives the user control/access to collector A functions as shown in Figure Pressing the Source Control key again will allow the user control/access to collector B functions. Pressing the Source Control key again will allow the user control/access to collector C functions. Pressing the Source Control key again will allow the user control/access to speed and temperature control functions as shown in Figure Figure 5-10: Collector Source Control Screen Figure 5-11: Source Control Screen Section 5 Programming 32

38 System Options The MicroettePlus System has a number of parameters that can be input by the user as part of the installation procedure. Figure 5-12 System Options Screen [1] Adjust Contrast This feature gives the user the ability to adjust the contrast on the LCD screen to accommodate ambient conditions. Press [ ] to make the display darker; press [ ] to make the display brighter. [2] Heater On/Off Press [2] to have the heater shutdown or stay on after the test is completed. [3] Validation The validation screen contains the following functions: System Serial Number Log -- Allows the user to input system serial numbers. To input or edit serial numbers, press [1] at the validation screen. The system will keep track of the date and username of the last revision. The revision and serial numbers may be printed using the print key. Cell Serial Number Log -- Allows the user to input cell serial numbers. To input or edit serial numbers, press [2] at the validation screen. There are multiple screens for multiple groups and styles of cells. Use the arrow keys to move to the different pages. Positions 7 and 8 are used to describe the cells. Maintenance Log This is where the instrument s critical life information is stored. To view or edit the maintenance log, press [3] at the validation screen. The system will keep track of the date and username of the last revision. The revision and maintenance log data may be printed using the print key. Maintenance Log Reset -- After maintenance has been conducted on any of these topics, the counts and dates should be reset. Do this by selecting the associated number and pressing the [Clr] key. The count will be reset to 0 and the date will be the current date. Section 5 Programming 33

39 [4] Calibration Volume Offset -- If volumes are not within tolerance, it is recommended to inspect for leaks, tubing clogs, syringe leakage or valve problems before changing the volume offset. The volume offset allows the user to increase or decrease the volume pumped by the syringes. This should only be changed after doing volumetric tests. Changing the volume offset affects all syringes and the volume they deliver. Use the symbol to reduce volume [5] Security When the security system is enabled, only personnel previously entered into the system by a manager may log on and access operational functions. This feature is highly recommended and, once in place, all personnel are required to log on to operate the unit. There may be up to 25 users programmed into the unit. There are two levels of users: Manager (open lock), who has complete access to all functions settings and operation of the unit. Operator (closed lock), who has limited access and may not modify any settings or protocols, but can start pre-programmed protocols. The operator may also view and print protocols and system settings. A manager can enable or disable password protection, add, modify, or delete a user, and change the timeout setting. A revision log is kept indicating when a setting was changed and who performed the change. The revision log may be displayed by pressing the clock key when the small clock icon appears at the bottom of any screen. NOTE: The security system may be disabled, although there will be no protection of important system settings and protocols. [6] Diagnostics When the [6] Diagnostics key is pressed, the control will go through and check the system for faults or failures. The information will be displayed on the screen and printed to the printer. Section 5 Programming 34

40 Setting Up and Starting the Security System With the Main screen displayed, Options screen. press [Preferences] to display the System Press [5] "Security. Highlight Manager and press [Enter]. Follow the directions at the bottom of the screen to initialize a password. Highlight Manager again and press [Enter]. Re-enter your password and press [Enter] [Enter]. You are now at the Manager Menu. Press [1] to enable the password protection. Press [4] to change the password timeout (default is five minutes). Press [2] add a user, press [1] add a manager, enter your name and press [Enter], press [Esc] [Esc] to Password screen, highlight your name which you just entered and follow the instructions to initialize your password, and then [Esc] to main menu. Press [Stop] [Stop] to log off and then press [Preferences]. You are now required to log on; highlight your name and press [Enter], enter your password and press [Enter]. You have now logged on. NOTE: Enable password and set time and date (see more in this section) before changing any system settings or creating protocols. This will ensure that the revision dates for these areas are correct. Adding, Changing or Removing Users When you are classified as a manager you may add managers or operators, and you may change an operator to a manager or a manager to an operator. To log into the Manager Menu from the Main Menu, press [Preferences], [5] Security, and then highlight your name and enter your password and press [Enter] [Enter] to view the Manager Menu. At the Manager Menu select Add a User to add a manager or operator. At the Manager Menu select Modify/Delete a User to remove or change someone in the list of users. Section 5 Programming 35

41 System Configuration [1] Device Setup Press the number for each of the devices to display their specific installation status. The system checks for these devices when the Diagnostic tests are performed. A failure is indicated if any of the specified accessories are not found. Figure 5-13: System Options Screen [1] - [4] Port Config To configure the sources. Most of the time, the source will be a diffusion cell group. The Port Config opens up a new screen for each port: [1] Configuration Configures the port for use. The available options are: None: When nothing is connected to this port. This port will be unavailable for test purposes. Cells: Used when sampling from a Hanson Research diffusion cell system. This setting is important because it allows the MicroettePlus to detect if the stirring control and collector are online. Other: Used when a non-hanson Research diffusion cell system is being used, or one with no communication capabilities. Section 5 Programming 36

42 Media: Used when the port is being used as the source of media. Normally port D is configured to Media.. [2] Collector Configures the fraction collector present on the diffusion cell group. The available options are: AutoFill: collector. Used when a Hanson Research AutoFill is used as a fraction MultiFill: Used when a Hanson Research MultiFill is used as a fraction collector. None: Used when a fraction collector is not installed on the cell group. [3] Stirrer Configures the stirring control present on the diffusion cell group. available options are: The MIXdrive: Used whan a VARIOMAG MIXcontrol controls the stirring. The MIXcontrol is typically a silver color. Telemodul: Used with a Telemodul stirring control. Typically these are blue. [5] Printer Set to Yes if a validation printer is plugged into Port 2 of the MicroettePlus. [6] Bath Heat To select the circulating waterbath installed on the system. The options are PS-8010, PS-8012, PS- 8012A, PS-AP07, Other. [7] System Defaults Used to change load speed, dispense speed, and stirring force. [2] System ID This ID is printed with the status information. It can be used to identify which system is being used, if the laboratory has more than one system. This is also used for identification when the system is operated remotely. [3] Edit Titles The Title Configuration screen is displayed, showing the choices that are available. To configure these titles: Select the type of title that is to be changed (company, header or protocol). The company name includes two lines of information, which are printed at the start of each test. These two lines are suggested to be the company and department names. The Header Titles will appear and will be printed at the start of a test. The Protocol Titles are located in protocols and printed at the start of each test. Select the corresponding number associated with the title(s) that are to be changed. Enter in the new title. Section 5 Programming 37

43 NOTE: Blank title areas are not printed. [4] Mode To select the Advanced or Basic user mode, press [4] to toggle back and forth. Advanced will allow the user to input scripting into the protocol. The scripting feature of the MicroettePlus is discussed later in this section. System Alarm/Timers With the Main screen displayed, press [Clock] to display the System Alarm/Timers screen. [1] Wakeup Alarm The Preheat Wakeup Time option allows the user to program the circulating waterbath to turn on the heater to a designated temperature at a designated time on a designated day, so that the bath temperature is equilibrated when the user is ready to use the system. The most typical use of this option is for the heater to be turned on approximately 1-hour before starting a test. To set the preheat wakeup time: Press [1] to select "Wakeup Alarm. The Preheat Wakeup Time screen is displayed. Input the time, date, recurrence and temperature. Follow each input with [Enter]. [2] Sampling Alarm This alarm is used to alert the user when a sample is to be taken from the vertical diffusion cells, as determined by the sampling interval set in the protocol. To set the parameters for the sampling alarm: Press [1] to select "Sampling Alarm. The Sampling Alarm screen is displayed. Press [ ] or [ ] to turn the alarm ON or OFF. The alarm is both audible and is indicated by the "Timer Alert" status light on the display. Set the pre-notification time. This sets the amount of time that the user is alerted before the sample needs to be taken. The range is 0-9:59 (Hours:Minutes) [3] User Alarms The system includes three undesignated alarms that the user can set to be alerted for an important event. Examples of user alarms include change the bath water, add algaecide, change the ph, or change the media. To set the user alarms: Press [1] to display the desired alarm number. Press [2] - [5] to input the time, date, a reminder of what the alarm is, and recurrence. Follow each input with [Enter]. When the date and time are reached, the user is alerted by an audible sound, a popup window on the screen and illumination of the "Timer Alert" status light on the display. Section 5 Programming 38

44 [4] Calibration Alarm The calibration alarm is used to alert the user when the system is due for validation. To set the calibration alarm: Press [4] to select "Calibration Alarm. The Calibration Alarm screen is displayed. Input the date and time for the next calibration. Follow each input with [Enter]. Use the arrow keys to select the recurrence. When the date and time are reached, the user is alerted by an audible sound, a popup window on the screen and illumination of the "Timer Alert" status light on the display. Repeating an Alarm (Recurrence) After setting alarms as previously described, the user may incorporate a recurrence for that alarm. Recurrence will automatically reset the alarm after the alarm is triggered. The date will change as selected and all other settings will remain the same. Follow the instructions on the display to select Disable (which will only trigger the alarm once), Mon-Fri, Mon-Sat, or the user can input the number of days for the recurrence (input 1 for everyday or 7 for once a week). Canceling an Alarm An alarm that has been set, but has not become due, can be canceled by displaying the input screen and pressing [Clr]. Acknowledging an Alarm When an alarm is triggered, the "Timer Alert" status light is illuminated and a pop-up warning window is displayed momentarily. The window is displayed again every few seconds and the audible beep is sounded until the alarm is acknowledged. To acknowledge the alarm: 1. Press [Clock]. The alarm pop-up window is displayed one last time. 2. Press any key to acknowledge the alarm and turn the "Timer Alert" light off. Clock Options With the Main screen displayed, press [Clock] to display the System Alarm/Timers screen. Press [5] to display the "Clock Options. [1] Time Format To change the time format from 12 to 24 and back. [2] Change Time To change the current system time. Section 5 Programming 39

45 [3] Date Format To change the date format, continue to press to review all date formats available. The system will use the format selected to display and print dates. [4] Change Date To change the current system calendar date. Print Options With the Main screen displayed, pres [Print Options] to display the Print Options screen. Figure 5-14: Print Options Screen [1] Print Status To print the current status of the MicroettePlus. [2] Print Protocol To select and print a protocol. [3] Print Header To print the header. [4] Print Last Test To Print Last Test. The last completed test is stored in the system buffer, each time a test is started this buffer is overwritten. Selecting and Viewing Protocol Parameters With the Main screen displayed, press [Edit/View Protocol] to display the Edit Protocol screen as shown in Figure With password enabled, managers may edit, while operators may only view. Section 5 Programming 40

46 Figure 5-15: Selecting a Protocol Creating a New Protocol NOTE: Password access may be required to modify protocol parameters. Input a protocol number, which is not displayed on the scroll; otherwise you would start editing the highlighted protocol and press [Enter]. The Operation Parameters screen is displayed, and a series of input screens are displayed. Input the desired parameters on each screen and move to the next screen by pressing [Enter]. Operation Parameters [1] Stirring Speed Input the speed at which you wish the stirrers to go, normally 400 rpm. [2] Temperature Input the temperature at which you wish the circulating waterbath to be set at, normally 32.5 C. [3] Tolerance Input the temperature tolerance you would like. This will only log to the printer if the circulating waterbath temperature should go outside this tolerance. Section 5 Programming 41

47 Sampling Parameters [1] Rinse Volume Input volume required to rinse sample lines. [2] Collect Volume Input volume to be collected. [3] Rack Type 12x32 and 16x100. Note that other rack styles are shown but not recommended by Hanson Research Corporation. Currently only evaporation caps/septums are available for the 12x32 and 16x100 test tubes. Sampling Time Parameters [1] Test Length Input the total time for the test. Range: 0-999:59 (Hours: Minutes). [2] Sampling Times This sets the number of times that the system will run the programmed sampling protocol. Range: 0-40 sampling points. [3] Pre-start Time Allows the user to set the MicroettePlus to start the sampling sequence cycle early (ahead of the scheduled sampling time) to meet sampling tolerance specifications. Default is zero. [4] Print Interval This allows the status conditions (elapsed time, system ID, speed, and temperature) to be printed at the designated interval during the test. Range: 0-99:59 (Hours: Minutes). Sampling Times This screen is used to specify the time when each of the samplings is to be taken. The sampling times are inputted as elapsed time. Notice that the screen displays all sample times with the current sample time highlighted. Input the time for the first reading and press [Enter]. Repeat for each time point. Range: 00:00 to 999:59 (Hours: Minutes). Protocol Labels Use [1] to [5] to input up to a 20-character label into the protocol titles. These descriptions are used for documentation purposes only and do not affect the functions of the instrument. Protocol titles can be customized using the Edit Titles screen. Editing a Protocol NOTE: Password access may be required to modify protocol parameters. 1. With the Main screen displayed, press [Edit/View Protocol] to display the Edit Protocol screen. 2. Use [ ] and/or [ ] to highlight the desired protocol and press [Enter]. Section 5 Programming 42

48 3. As each of the parameter selection screens are displayed, any of the parameters can be changed by selecting the number associated with that parameter. Once the number has been selected the value will change or go blank, allowing a new value to be entered. Viewing Protocol Parameters 1. With the Main screen displayed, press [Edit/View Protocol] to display the Edit Protocol screen. 2. Use [ ] and/or [ ] to highlight the desired protocol and press [Enter]. 3. The Operation Parameters screen is displayed. Notice that the selected protocol number is displayed in the folder on the lower left-hand corner of the screen. 4. Press [Enter] to step through the selection screens and view protocol parameters. Printing a Protocol 1. With the Main screen displayed, press [Print Options] to display the Print Options screen. 2. Press [2] to select "Print Protocol." The Select Protocol screen is displayed. 3. Highlight the desired protocol and press [Enter]. The protocol parameters are printed. Working with Scripts Definitions A script is a list of procedures that the MicroettePlus implements during a sampling event. From the parameters selected by the user (Rinse volume, Collect volume, etc.) the system generates a script which it follows every time a sampling event takes place. Since the script is generated automatically and transparently, most users will not need to concern themselves with it. Changing the script is only necessary if the standard sampling steps are not adequate. An example would be that after the 3 rd hour of a test, the circulating waterbath temperature must be increased by 3 C. Section 5 Programming 43

49 Enabling Scripting To enable editing of the script, press [Preferences], select option [7] System Configuration and press [4] to select advanced mode. Creating a Script Press [File] and select a new or existing protocol. While in the sampling parameters window, press that these parameters generated. [File] to view the script Figure 5-16: Edit Protocol Script Screen The MicroettePlus converted the protocol parameters entered into the script shown above in Figure This simply means that at each sampling event, the MicroettePlus will repeat these steps. It will first rinse the lines with 1.5 ml of the sample. Then it will collect 1.0 ml of the sample into the collection rack. Available Script Procedures The power of scripting is not so much that you can view the script, but that you can now edit the script by adding and rearranging it with additional procedures. The available procedures are: Collect Volume Delay Print Prompt Collect Volume volume to be collected from current cell. Rack Type rack type for collection. Delay how long to wait before starting next script item. Occurrence when procedure is implemented. Data what information to sent to the printer. Occurrence when procedure is implemented. Message message used to prompt user with popup window. Delay how long to wait if user does not acknowledge. Occurrence when procedure is implemented. Section 5 Programming 44

50 Rinse Volume Transfer Stirrers Bath Heat Rinse Volume volume used to rinse lines before sampling. Load Speed stepper speed while loading syringes. Dispense Speed stepper speed while dispensing syringes. Drain Delay sets the time the unit will hold before the next step, allowing time for the liquid to stop moving. Source where transfer solution is obtained. Target where transfer solution is sent. Transfer Volume amount of solution transferred. Occurrence when procedure is implemented. Stir Speed the new speed the current stirrers will go to. Occurrence when procedure is implemented. Bath Heat the new heat setting the bath will go to. Occurrence when procedure is implemented. Modifying an Existing Script An example of modifying a script: 1. To take the current script and modify it so that the system prints the circulating waterbath conditions every time a sample is taken, first go to the Edit Protocol Script screen and highlight under the second item by using the [ ] key. Figure 5-17: Edit Protocol Script Screen 2. Press the [.] key to insert a new procedure before the highlighted item. 3. Press the [ ] key and highlight the Print procedure. 4. Press [Enter] to select the procedure. The parameters associated with the procedure are displayed. Figure 5-18: Edit Print Procedure Screen Section 5 Programming 45

51 [1] Data Key to toggle between the three data options: (a) MicroettePlus Status, which prints the current status of the MicroettePlus, (b) Bath Status, which prints the status of the circulating waterbath, or (c) Message, which prints a user-defined message to the printer. Select Bath Status. [2] Occurrence To toggle between the five occurrence options: (a) Always, which means it prints the data every time the script runs, (b) Group, which means that it prints the data once per group of cell groups. In other words, if three cell groups are running, only the third one will implement this step, (c) OnEvent, means the print script will only be implemented at the selected sampling interval, (d) Start, means the print procedure will only run once at the start of the test, and (e) End, means the print procedure will only run once at the end of the test. Select Always. Figure 5-19: Edited Script Uses for Scripting Using scripting, advanced users can use the transfer function. The transfer function allows the user to move a programmed volume of fluid from a source to a target location at a selected time during or after the test. Several transfer procedures can be placed one after the other for cell dilution requirements. Use the print function to print MicroettePlus status or a user-defined message. If you require collection of more sample than the vial holds or if you want two collected samples for two different analyses, place two collect volume procedures one after the other. The MicroettePlus will collect the volume specified by the first procedure, move to the next row and collect the amount specified by the second procedure. Use the prompt command to remind users of a specific task that has to be performed. The user is prompted by a popup window which can be programmed to remain for a certain amount of seconds or wait for user acknowledgement. Use the occurrence parameter to have more control over when the script functions are implemented. Section 5 Programming 46

52 Running a Test The user runs a test by simply starting a protocol stored in memory. Starting a protocol has the following advantages: Ensures that critical test parameters are selected correctly every time and cannot be changed by the operator. Enables the user to monitor temperature tolerances during the test. Automatically prints pertinent test data at the beginning of the test. Allows the user to program sampling alarms. The system may be easily programmed to automatically shut down the circulating waterbath after the test ends. Starting a Protocol The MicroettePlus is designed to perform a test using a set of parameters stored in a protocol. Press [Run]. The Select Protocol screen is displayed. Figure 5-20: Select Protocol Screen Input the protocol number or highlight the desired protocol and press [Enter]. Input header information and press [Enter]. The run options screen is displayed. Press the corresponding number to select the cell groups for testing, the starting row for the MultiFills, and the group offset time. Press [Enter] when done. Figure 5-21: Run Options Screen Section 5 Programming 47

53 The system is now in the setup mode. The system will check and diagnose that the setup and equipment specified are ready. Note that this process is not completely foolproof. Equipment that cannot communicate and that does not have feedback, should be physically checked to ensure it is ready and setup properly. 0:00 Figure 5-22: Start Test Screen Press [ ] to display the Set Point screen, as shown in Figure Figure 5-23: Set Point Screen The Set Point screen displays the timing interval for Auto Print, the speed the stirrers are set to, and the temperature the circulating waterbath is set to. The Set Point screen also indicates which of the alarms have been set. If any parameter is incorrect, press [Stop] twice to abort test. Section 5 Programming 48

54 Press [ ] to display the Header screen, as shown in Figure Figure 5-24: Header Data Screen Verify the user's name and other header information. Change header information as needed. The header information, the "Configure Title" information, and the protocol parameters are printed at the start of the test. Press [ ] to display the Status screen. When parameters are within tolerance, a pop-up screen appears displaying Press [Run] To Start. 0:05 29:55 Figure 5-25: Main Menu Screen with a test running Press [run] to start the first cell group test. If running a second Multi-Group test, the sample timer will count down the offset time to start the second cell group. When the pop-up window displays Start Test 2, Press [Run] to start the second cell group test. If running a third Multi-Group, the sample timer will count down the offset time to start the third cell group. When the pop-up window displays Start Test 3, Press [Run] to start the third cell group test. If Auto Print was selected, the status conditions are printed at the interval specified in the protocol. The number of samplings and the remaining time until the next sampling are displayed on the Status screen. If the sampling alarm is turned on, when each sampling interval is reached an audible alarm sounds, a warning window displays momentarily, and the "Timer Alert" light illuminates. Once all cell groups have been started, the elapsed time will display the total test run time from the start of cell group 1. The event time will display the countdown time to the next event (sampling interval). You will notice that in the diagram of the cell the magnet is turning, indicating that the stirrer is operating. Section 5 Programming 49

55 When the event has counted down, the sampling sequence will take place and you will notice the stirring stop. Other information will appear on the screen indicating what steps are taking place. 0:05 29:55 Figure 5-26 Test Running Screen NOTE: The timers will display hours and minutes when the value is 1-hour or more. The timers will display minutes and seconds when the value is less than 1- hour. When all cell group tests are finished, the display will show: Test Complete Press Any Key Stand By Mode/Log Off When not running a protocol, press [stop] to put the unit in the stand by mode. Press any key to wake the unit up. This is also a fast way to log off if you are using the security system. Section 5 Programming 50

56 Section Six Operation and Setting Up Overview One of the most important factors to ensure a successful test is to deaerate the replace solution prior to the test. The following instructions are suggested, but modifications to meet individual requirements are acceptable. Always use the same magnet and helix with the same cell. Cell volume should always be validated with the magnet and helix in place. Syringe Priming Prime the syringes with at least 4 ml of the deaerated media before starting the test. Cell Preparation Bubbles under the membrane are an area of concern with vertical diffusion cells. Proper deaeration of the media and proper placement of the dosage wafer will ensure bubble-free testing. See the Figures in Section 4 Installation for parts identification. With cells in cell holders and water jacket tubes connected: 1. Prepare the membranes. 2. Ensure magnet and helix are in place in each cell. 3. With the replacement line connected to the bottom of the cells, pre-fill each cell with media until the cell is 90% filled. The bubble trap may have a few bubbles; this is acceptable. 4. Ensure there are no bubbles in the cell. If bubbles are noticed, run the stirrer at 1000 rpm for a few seconds to release any bubbles. You may also insert a probe into the cell to help release bubbles. 5. Continue to fill each cell until there is a positive meniscus covering the complete top of the cell. Allow a few minutes for the cell to warm to operating temperature. 6. Always ensure that the stirring is OFF before you place the wafer on the cells. Place dosage wafer on cell; do this by holding the wafer with thumb and forefinger at about a 30 angle to the top of the cell. Place the thumb and edge of the wafer on one edge of the cell and then immediately roll the wafer onto the top of the cell. Inspect for bubbles beneath the membrane. 7. Place glass disk and then cell ring on cell and align side cut out of ring for viewing into the cell if desired. 8. From the side, slide clamp onto cell, over the cell ring and under the cell flange. 9. Start the test (see Section 5 Operating Instructions & Programming ). Section 6 Set-Up and Operation 51

57 Figure 6-1 Cell Preparation Section 6 Set-Up and Operation 52

58 Stirrer Control Review the Control Settings in the Variomag/2mag stirrer control operation manual. The stirrer control is directed by the MicroettePlus. The settings within the protocol and MicroettePlus system setup ensure the stirrer control s operation. When connected to and controlled by the MicroettePlus, the upper portion of the stirrer control display will read REMOTE. Recommended Settings: Speed = 400 and Power = 50%. Never run the unit with the force or power turned up over 50% which may cause the stirring drive to warm up and, in turn, warm up the cells past the required temperature. Figure 6-2: Stirrer Control Circulating Waterbath Review the Control Settings in the PolyScience circulating waterbath operation manual. Model AP07 has been designed for connection and interfacing with the Hanson Research MicroettePlus; other circulating waterbaths will not communicate with the MicroettePlus. The settings within the protocol and MicroettePlus system setup ensure the circulating waterbath s operation. Setting the pump speed to 10% is recommended; this is done through the keypad of the circulating waterbath. The displayed temperature range reading of the bath temperature may also be set, and setting the reading to one place (XX.X) is recommended. These settings are detailed in the waterbath s operation manual. NOTE: Baud Rate must be set to 9600 to communicate with the waterbath. Ambient conditions and circulating waterbath settings may affect temperature. Check the minimum temperature operation of the waterbath before you do any testing. By setting the bath temperature to 20 and monitoring the waterbath s displayed temperature, ensure the temperature does not exceed the setting requirement. Section 6 Set-Up and Operation 53

59 The circulating waterbath will need to be set above the goal temperature of the cells. The temperature difference between the setting and the actual temperature is dependent on ambient conditions. This difference should be tested. Starting and Running a Test Prior to starting and running a test, this operation manual should be read and the unit should be set up and tested for proper programming and operation. Please ensure these steps have been taken, and then proceed to the following: 1. Verify that the stirring drives are ready to go, all required specifications are within tolerance, and all hardware settings are ready. 2. Verify that the circulating waterbath is set to the desired temperature and is controlling at that temperature. 3. Prepare the membranes, as well as an extra membrane (suggested). 4. Verify that the media source is deaerated and to temperature and that the MicroettePlus syringes through to the cells have been primed with the new media. 5. Verify that the Collection Rack is in place and has the correct amount of vials. 6. Verify that the Validation Printer is powered ON, is on-line, and has paper. 7. Verify that the cells are filled and at the required temperature. 8. Select the desired protocol. 9. Apply the wafers, cell tops, and clamps to the cells. 10. Press the START key (the beep is time 0 ). 11. When running a Multi-Group test, wait for the second beep (A-B offset) for the start of the B side cells. Apply the tops to the next group about two minutes before the start of each subsequent group. The 2 nd and 3 rd groups will start after the group offset countdown and pressing [Run]. What Will Happen During a Test The basic sampling procedure is performed by following the steps listed below; variations may be evident, depending on how the protocol has been programmed. After setting up and starting a protocol: 1. The MicroettePlus will check important system settings, ensuring the circulating waterbath, stirrer control(s), Validation Printer and collector(s) are ready. The MicroettePlus will not allow a test to start unless all equipment and test parameters are ready and within tolerance. 2. The timer will start and count down to the sampling interval ( Event ). Section 6 Set-Up and Operation 54

60 3. Just before each of the sampling intervals, the MicroettePlus will load the syringes with media from source D and stop the stirrer control for that group. 4. At the sampling interval time (less the pre-start time), the MicroettePlus will deliver the programmed amount of rinse media to the cells and through to the collector of Group A and then on to waste. This process will repeat for each group. 5. After the rinse volume has been delivered, the collector will move to the next available row to collect the programmed amount of sample, then return to the home position to wait for the next interval. This process will repeat for each group. 6. The MicroettePlus will continue to count down to the next sampling time point and repeat steps 2 through 5 above until all programmed intervals have been completed for each group. Critical test information will be sent to the Validation Printer via the MicroettePlus throughout the sampling procedure described above. Printed Information The Validation Printer will print critical information during a test, generated as a group of information followed by the System ID and Date printed. Some data will also include Revision information. Most all of the system settings and protocol information can also be sent to the Validation Printer for a hardcopy report of important information. This includes System Setup and Serial Numbers. Elapsed time printouts are in HH:MM:SS (hours:minutes:seconds). At the beginning of the event, the elapsed time will be printed followed by the function or process that is taking place. Example Printout (with explanation) HANSON RESEARCH CORP (your company name here) DEPARTMENT: QUALITY ASSURANCE (your department here) System ID: MicroettePlus (this is the identification that you can assign) ---PROTOCOL #1 START--- (the protocol selected to run) 29.AUG :12 (the time the protocol was started) USER NAME: Bill Smith (the person who is logged on and started the test) SAMPLE ID: 1a (header titles and labels) LOT NUMBER: 34X564 (header titles and labels) EXP. DATE: Feb 04 (header titles and labels) COMMENTS: None (header titles and labels) ---PROTOCOL #1--- Rev: 29.Aug.2001 (revision date of protocol) By: Bill Smith (person who revised or created the protocol) The following information lists the parameters programmed for the above protocol Stirrer Speed: 400 rpm (the set speed for the stirrers) Bath Set Temperature: 32.5 (the set temperature for the circulating waterbath) Section 6 Set-Up and Operation 55

61 Temperature Tolerance: 2.0 C (the temperature tolerance allowed) If the temperature should go outside this range, the system will log the information to the Printer. When/if the temperature returns back into tolerance it will also be logged. Rinse Volume: 2.0 ml (the amount of volume programmed to rinse the sampling lines) Collect Volume: 0.5 ml (the amount of volume programmed to collect) Rack Type: 12x32 (the Collection Rack style mounted in the MultiFill) Test Length: 6:00 (the overall test length time) Number of Sampling Times: 5 (the number of times the unit will sample) Pre-Start Time: 45 Sec (the time before the actual sample time point that the unit will begin the sampling process) Auto Print Interval: 1:00 (the time at which the unit will print status) Sampling Times: 1) 0:30 2) 1:00 3) 2:00 4) 4:00 5) 6:00 PROTOCOL ID: 6 hr Test (protocol titles and labels) PRODUCT: Hydrocortisone (protocol titles and labels) DOSAGE: 300 mg (protocol titles and labels) MEDIA: 30% Alcohol (protocol titles and labels) MEMBRANE: 0.45 um (protocol titles and labels) MultiFill Starting Row: 1 (the row the MultiFill will start collecting in) Script Procedures: 1 Rinse Volume 2.0 ml 2 Collect Volume 0.5 ml 12x32 SELECTED: GROUP A (this is the group that has been selected to run) ELAPSED TIME: 00:00:00 (HH:MM:SS) (at this time the following process took place) GROUP A START 400 rpm (group A started and is running at 400 rpm) ELAPSED TIME: 00:29:16 EVENT# 01 (at this time the following process took place) GROUP A: RINSE 2.0 ml (volume pumped to rinse the sampling lines) ELAPSED TIME: 00:30:44 EVENT# 01 (at this time the following process took place) GROUP A: COLLECT 0.5 ml Row 1 (volume pumped collected in row 1) The test will continue and print out at each sampling time point. At the end of the test the unit will print the waterbath information if you have Model 8010 or 8012: MIN MAX AVERAGE BATH TEMP: 32.5 C 32.9 C 32.6 C (bath temperature readings during the test) ---PROTOCOL #1 COMPLETED--- (the protocol has been completed) The preceding is a typical printout; variations may be evident depending on how the system is set up and how the protocol has been programmed. Cleanup Proper cleanup of the system is very important. Sample material should not be left in the system for long periods of time (more than four days). System cleanup immediately following a test (or as soon as possible) is recommended. Leaving all cells and tubing connected at all times is also recommended as it reduces the chances of breaking cells or inter-mixing cell components. Section 6 Set-Up and Operation 56

62 Cell Top At the end of the test, dismantle the samples from the diffusion cells. Remove the clamps, tops and membranes. Clean the Teflon washers and their glass tops by removing the ointment or cream with a tissue and wash them in warm water. Finally, rinse the cell tops with alcohol and allow to air dry. Figure 6-3: Vacuum Pump and Flask The Vacuum Pump and Flask are accessory items that are part of the Start-Up Tool Kit. Connect the system as shown and turn the power switch ON. Use this device or one similar as follows: NOTE: The following steps are recommended and may be changed to fit individual requirements. This process must be completed for each group. Cell and Helix 1. Use the stainless probe tip and place it into the cell to drain. Rinse this cell with alcohol while continuing to drain, and allow the magnet and helix to turn. 2. Remove helix and rinse over cell with alcohol. Rinse and drain cell until satisfied that it is clean. 3. Replace helix and fill cell to near top. 4. Repeat for each cell, ensuring magnet and helix have been replaced into the original cell. Sample Tubing 1. Remove the collection rack from the collector and with manual control, move the needle array of the collector out to the last row and down (see Section 5 Operating Instructions & Programming ). Section 6 Set-Up and Operation 57

63 2. Use the flexible probe tip and push over Needle #1. This will now draw alcohol from the cell along with the last sample through the sample tubing. Continue until air is drawn through the sample tubing. Repeat as necessary. 3. Repeat for each position. Replacement Tubing (same media) 1. At the start of a new test, place media probes into new media. (Rinsing the old media out is only necessary when starting a new test using the same type of media.) 2. Pump adequate volume to rinse the old media (from the last test) into the cell 4 ml is normally enough. 3. Drain this old media from the cell. 4. Repeat as necessary when changing to a new media. Plastic Vessel Never allow the vessel to fill more than ¾ full. Lift the cover from the plastic flask and remove material from inside. Section 6 Set-Up and Operation 58

64 Section Seven Troubleshooting Contacting Hanson Research Corporation for Technical Support For technical support, please submit a TSR form located at or techsupport@hansonresearch.com. If you require phone support, please call or and select the option for Technical Support. Please have the following information available: 1. Instrument Type 2. Serial Number 3. Firmware Version 4. What is the system configuration? How is the Vision Microette connected to other equipment? 5. How long did the equipment work before the problem occurred? 6. How old is the equipment? 7. Were there any recent repairs or maintenance on the unit? 8. How is the system being used? What is the application? 9. Detailed information about the problem. What is working and what is not? 10. The troubleshooting steps that have already been taken Section 7 Troubleshooting 59

65 Electrical Troubleshooting Problem Possible Causes Recommended Action The display is dark. The system is in standby (the Power On light is illuminated). Press any key to exit the standby mode. The system is not plugged in or turned on (the Power On and CPU lights are off). Verify that the power cord is attached to the back of the controller and that the power switch is turned on. The MicroettePlus is not operational (the Power On light is Illuminated). Change the fuses in the MicroettePlus (see Section 8 Maintenance ). If the CPU light still does not come on, contact Hanson Research. The power light on the front panel is not illuminated. The system is not plugged in or turned on. Verify that the power cord is attached to the back of the MicroettePlus and that the power switch is turned on. Bad fuse. Check or replace the fuse (see Section 8 Maintenance ). Power supply is defective (no light on power supply). Check or replace the power supply. The display is not clear. Contrast is not set correctly. Adjust contrast (see Section 5 Operating Instructions & Programming ). Loss of programmed information when power is turned off and then back on. Battery failure. Replace smart block module on main board. Section 7 Troubleshooting 60

66 Liquid Flow Troubleshooting Problem Possible Causes Recommended Action Sample volumes are incorrect. Not using vial caps. Leaking syringe. Use recommended vial caps. Check and replace syringe. Offset volume not set or set incorrectly Check and set offset volume. Leaking fittings Check all fitting connections for leaks and fix or replace any leaking fittings. Excessive carry-over. Inadequate rinse volume. Increase rinse volume to a minimum of 2 ml. Drug loss. Sample absorbing to parts of the liquid flow path. Evaluate wetted materials; sample may be absorbing them. (see Section 3 Specifications for wetted parts) Section 7 Troubleshooting 61

67 Validation Printer Troubleshooting NOTE: The Hanson Research Validation Printer comes with its own operation manual. Please be sure to read this manual in its entirety and then review the troubleshooting guide below. Problem Possible Causes Recommended Action Alarm light always blinks. Low or no paper supply. Replace roll of paper. Prints a little and then the alarm light blinks. Low paper supply. Paper is incorrectly installed. Replace roll of paper. Check paper to see that it is installed correctly and that the paper indicator button is not stuck (see Validation Printer Operation Manual ). Printer will not print at all. Low paper supply. Replace roll of paper. Paper is incorrectly installed. Check paper to see that it is installed correctly and that the paper indicator button is not stuck (see Validation Printer Operation Manual ). Printer is not connected. Connect the printer (see Section 4 Installation ). Printer Self Test To run a Self Test, turn the power OFF, hold down the LF button, and turn the power back ON. When the unit begins to print, release the LF button. The printer should print its ID and that it is printing a test pattern. The test pattern consists of all printable characters in black and red. Section 7 Troubleshooting 62

68 Printer Set-Up Test To run a Set-Up Test, turn the power OFF, hold down both the LF and SEL buttons, and turn the power back ON. When the unit begins to print, release both buttons. The printout should read: DP614 POWER ON SELECT CR = CR France DRAWER SERIAL INTERFACE 8 BIT NON PARITY 9600 NATIVE MultiFill Troubleshooting Problem Possible Causes Recommended Action Needle Error message on display. Bad up sensor. Check and replace up sensor. Broken belt. Remove cover and inspect belt. Replace if necessary. Shuttle Error message on display. Bad home sensor. Check and replace home sensor. Bad motor. Check and replace motor. MicroettePlus does not communicate with one or more MultiFill(s). Communication connection. Not set up. Check communication connection(s). Ensure the MicroettePlus has been set up to know that there is a MultiFill(s). Section 7 Troubleshooting 63

69 Stirrer Control/Temperature Control Troubleshooting Problem Possible Causes Recommended Action MicroettePlus does not communicate with one or more stirrer control(s) or Temperature Control(s). Communication connection. Check communication connections. Not set up. Ensure the MicroettePlus has been set up to know that there is a stirrer control(s) and Temperature Control(s). Power connector came out of ComSwitch Check power connector on the ComSwitch. Wrong stirrer control specified Configure system for use of proper stirrer control through Device Setup screen. Circulating Waterbath Troubleshooting Always make sure waterbath is set to 9600 baud rate. Section 7 Troubleshooting 64

70 Section Eight Maintenance Maintenance Schedule Procedure Inspect syringes visually. Frequency Once a week. Replace syringe at 100,000 cycles or if a leak is detected. Clean sample lines. After each test. Clean exterior. As needed. Clean up spillage. Immediately. Change/clean the water in the circulating waterbath. Every month, or as needed. Inspect sample lines and connections. Every six months or whenever changed. Inspect operation and needles on each collector. Every six months. Inspect MicroettePlus operation and validate delivery volume. Every six months. Inspect drive and belt on each MultiFill. Every year. General Maintenance The MicroettePlus and MultiFill have been designed to be low maintenance instruments. In general, the instruments are maintained by wiping clean after each use. Frequency of cleaning depends on instrument usage and operating environment. If there is spillage on or near the system, clean it up as soon as possible. Section 9 Moving and Storage 65

71 Fuse Replacement CAUTION For continued protection against risk of fire, replace the fuse(s) with same type and current rating. Disconnect power cord before changing fuses. To check the fuses and replace a defective fuse in the MicroettePlus: 1. The fuse is located on the back of the MicroettePlus. The system has one fuse. The amperage is labeled next to the fuse. The fuse holder has a finger-grip knob; push in this knob, slightly turn it counter-clockwise, and then pull knob with fuse straight out. 2. Check the fuse with an OHM meter. If you are not sure, or if you do not have an OHM meter, replace the fuse. 3. If the fuse is defective, remove the fuse from the holder by pulling it straight out. Replace with a fuse of the same type and rating. (see Section 3 Specifications ) 4. Push the fuse back into the MicroettePlus. Secure the fuse holder by pressing in and slightly turning clockwise. To check the fuse(s) and replace a defective fuse in the MultiFill(s): 1. The MultiFill fuses are located on the side of the unit where the power cord is connected. Each unit has one or two fuses. With a small bladed screwdriver, gently pry the cover/fuse block away from the power entry port housing and remove. 2. Check the fuse(s) with an OHM meter. If you are not sure, or if you do not have an OHM meter, replace the fuse in question. 3. If a fuse is defective, remove the fuse from the holder by pushing it out towards the side. Replace with a fuse of the same type and rating. (see Section 3 Specifications ) 4. Slide the cover/fuse block back into the power entry port housing the same way that it came out. Snap the cover/fuse block assembly securely into place. Syringe Replacement Replace syringes when maintenance log indicates 100,000 cycles. replacing syringes, volume validation should be performed. After 1. Remove shoulder screw at the bottom of syringe. 2. Grasp the barrel with your finger and turn to unscrew the syringe end from the manifold. Remove Teflon spacer from inside the manifold and replace with the new one supplied with syringe. Section 9 Moving and Storage 66

72 3. Install the new syringe into the manifold finger tight and replace shoulder screw. Volume Validation Use HPLC grade water at room temperature. 1. Weigh empty sample collection vials with pre-cut caps (required) and record. Do at least three sets. 2. Program the MicroettePlus to collect the required sample volume. 3. Weigh full sample collection vials and subtract the empty value from the full. Make any calculations required for water density. Cell Volume Evaluation Though other methods may be used, the following steps are suggested: 1. Place the helix and magnet in a dry cell. Place a piece of parafilm on the top of the cell. Assemble the cells with the glass disk on top of the parafilm and the wafer on top of the glass disk, and place the ring and the clamp on the cell. 2. Place the plug on sample port of cell. 3. Weigh the cell (empty). 4. Remove the plug and while holding the cell with the sample and replace ports pointing up, fill the cell through the sampling port with the syringe supplied, until the cell is filled. Do not fill the bubble trap. 5. Place plug on replace port of cell and then remove the filling syringe. 6. Weigh the cell again (full). Repeat at least twice more to ensure accuracy. Syringe Speed Adjustment The speed at which the syringe will fill and deliver the replacement media solution into the cell may be modified. Changing the factory set speed may be advantageous if the replacement solution is more viscous than normal D.I. water. Slowing down the syringe pump will reduce the pressure build-up on the cell membrane. The syringe cycle consists of a Load and Dispense phase. Changing the syringe speed can be done through the keypad on the MicroettePlus (see Section 5 Operating Instructions & Programming ). Factory default settings during testing: Load at 500 and Dispense at 60. Factory default settings while priming: Load at 500 and Dispense at 500. Section 9 Moving and Storage 67

73 NOTE: The syringe dispense speed of 60 was selected to ensure a minimum pressure build-up in the cell. This speed matches the speed of liquid delivery from the previous Microette system. Speeds may be increased for faster throughput if desired. Be aware that increased speeds may cause leaking around the membrane or cell top. Always ensure that these increased speeds are not affecting test results. MultiFill Needle Replacement Visually inspect the needles on each MultiFill monthly and replace as necessary. 1. Remove the collection rack from each MultiFill. From the keypad of each MultiFill, move the dispensing needles out to the end row. 2. Grasp the plastic top of the needles and twist to unscrew. The needles will come away from the connector. 3. Insert new needles as necessary and twist until tight. Repeat for each needle. 4. Run a few pumping cycles on each MultiFill with new needles to ensure proper operation and that there are no leaks in the valves at the home position. MultiFill Belt Inspection and Replacement The MultiFill has a belt that raises and lowers the needles. This belt should be inspected each year. Prior to doing any work, turn off each MultiFill and unplug the power sources. Then, follow these steps to replace the belt(s) of each MultiFill: 1. Remove the collection rack and upper cover to provide access to the drive components. 2. Rotate the drive plate lead screw by hand to position the carriage to the front of the unit. 3. Rotate one needle drive pulley by hand to position the needles in the up position. 4. Remove two flat head screws from under the drive plate assembly. These screws retain the main ACME nut, mounting block. NOTE: A nine inch long screw driver works best. 5. Slide the drive plate assembly away from the ACME nut mounting block. 6. Loosen the idler pulley screw using a 5/16 wrench and move the pulley to the fully retracted position. 7. Loosen the four screws which secure the drive motor and slide the motor forward to loosen the belt. 8. Remove the old belt and install new belt P/N Section 9 Moving and Storage 68

74 9. Reinstall the ACME screw mounting block and secure with two flat head screws. 10. Slide the drive motor back to remove belt freeplay and tighten four motor screws. 11. Adjust idler pulley position to provide final belt tensioning. A properly adjusted belt may be easily deflected about 1/8 inch at midpoint between two pulleys. 12. Rotate the main drive screw manually to verify proper operation. 13. Manually rotate one of the needle drive pulleys and verify proper up/down needle operation. 14. Replace upper cover, install collection rack, and reconnect power source. 15. Operate the instrument as necessary to ensure proper operation. Needle Replacement It is recommended to replace the PEEK needles of the AutoFill annually or as needed. 1. Remove the AutoFill rack. 2. Using collector controls, move the AutoFill to the service position. 3. Gently pull forward and down on the top of the dispense head cover to remove it. 4. Disconnect each of the PEEK needles from the needle block. 5. Secure the dispense head cover. 6. Using the Vision Microette controls, return the AutoFill dispense head to the home position. Section 9 Moving and Storage 69