Beckman Coulter System Reagent, AU400/AU400 e LITHIUM Cat. No.: OSRT056 Reagent ID: 600

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1 Beckman Coulter System Reagent, AU400/AU400 e Test Name: Li < > Type: Serum Operation: Yes Sample: Volume 2 μl Dilution 0 μl Pre-Dilution Rate: 10 Reagents: R1 Volume 150 μl Dilution 0 μl Min OD Max OD R2 Volume 0 μl Dilution 0 μl L H Reagent OD limit: Wavelength: Pri. 520 Sec. 480 First L -0.5 First H 0.80 Method: END Last L -0.5 Last H 0.80 Reaction slope: - Dynamic Range: Measuring Point 1: First 0 Last 6 L 0.1* H 5.0* Measuring First Last Correlation Factor: Linearity: % A 1 B 0 No Lag Time: On-board stability period: 14 Test Name: Li < > Type: Serum Value/Flag: # Level L: # Level H: # Normal Ranges: Age L Age H Sex Year Month Year Month L H ο 1. # # # # # # # ο 2. # # # # # # # ο 3. # # # # # # # ο 4. # # # # # # # ο 5. # # # # # # # ο 6. # # # # # # # 7. None Selected # # 8. Out of Range L H # # Panic Value: # # Unit: mmol/l* Decimal places: # Test Name: Li < > Type Serum Calibration Type: AB Formula: Y=AX+B Counts: # Process: CONC Cal. No. OD CONC Factor/OD-L Factor/OD-H Point 1: # * 3.0* 7.0* Point 3: Point 4: 1-Point Cal. Point: With CONC-0 Slope Check None Advanced Calibration: ## MB Type Factor: Calibration Stability Period: 7 # User Defined ## Lot or Lot + Bottle * Units are in mmol/l. To work in meq/l multiply by 1.0! Do not use Lithium Heparin plasma samples

2 Beckman Coulter System Reagent, AU640/AU640 e Test Name: Li < > Type: Serum Operation: Yes Sample: Volume 2 μl Dilution 0 μl Pre-Dilution Rate: 10 Reagents: R1 Volume 150 μl Dilution 0 μl Min OD Max OD R2 Volume 0 μl Dilution 0 μl L H Reagent OD limit: Wavelength: Pri. 520 Sec. 480 First L -0.5 First H 0.8 Method: END Last L -0.5 Last H 0.8 Reaction slope: - Dynamic Range: Measuring Point 1: First 0 Last 6 L 0.1* H 5.0* Measuring First Last Correlation Factor: Linearity: % A 1 B 0 No Lag Time: On-board stability period: 14 Test Name: Li < > Type: Serum Value/Flag: # Level L: # Level H: # Normal Ranges: Age L Age H Sex Year Month Year Month L H ο 1. # # # # # # # ο 2. # # # # # # # ο 3. # # # # # # # ο 4. # # # # # # # ο 5. # # # # # # # ο 6. # # # # # # # 7. None Selected # # 8. Out of Range L H # # Panic Value: # # Unit: mmol/l* Decimal places: # Test Name: Li < > Type Serum Calibration Type: AB Formula: Y=AX+B Counts: # Process: CONC Cal. No. OD CONC Factor/OD-L Factor/OD-H Point 1: # * 3.0* 7.0* Point 3: Point 4: 1-Point Cal. Point: With CONC-0 Slope Check None Advanced Calibration: ## MB Type Factor: Calibration Stability Period: 7 # User Defined ## Lot or Lot + Bottle * Units are in mmol/l. To work in meq/l multiply by 1.0! Do not use Lithium Heparin plasma samples

3 , AU480 Beckman Coulter System Reagent: OSRT056 Test Name: Li < > Type: Serum Operation: Yes Sample Volume 2 μl Dilution 0 μl OD Limit Pre-Dilution Rate 10 Min. OD Max. OD Reagents Volume: R1(R1-1) 150 μl Dilution 0 μl Reagent OD limit: R2 (R2-1) 0 μl Dilution 0 μl First Low -0.5 High 0.8 Last Low -0.5 High 0.8 Dynamic Range Low 0.1* High 5.0* Correlation Factor A 1 B 0 Wavelength: Pri. 520 nm Sec. 480 nm Factor for Maker A 1 B 0 Method: END Reaction slope: - Onboard Stability 14 Day 0 Hour Measuring Point 1: First 0 Last 6 LIH Influence Check # Measuring First Last Lipemia Linearity: % Icterus No Lag Time: Hemolysis Range Test Name: Li < > Type: Serum Value/Flag: # Level L: # Level H: # Specific Ranges: From To Panic Value Sex Year Month Year Month Low High Low High 1. # # # # # # # # # 2. # # # # # # # 3. # # # # # # # 4. # # # # # # # 5. # # # # # # # 6. # # # # # # # 7. No demographics # # 8. Not within expected values # # Unit mmol/l* Decimal Places # Test Name: Li < > Type Serum Use Serum Cal. Calibration Type: AB Formula: Y=AX+B Counts: # <Calibrator Parameters> Slope Check None Calibrator OD Conc Factor Range Low High Allowable Range Check Point 1: # 3.0* 7.0* Reagent Blank Calibration Point 3: Advanced Calibration Point 4: Operation Yes Interval (RB/ACAL) Lot / Lot Point 8: Point 9: Point10: <Point Cal. For Master Curve> No. of Correction Points Use Master Curve Lot Calibration Calibrator OD Conc OD Range Stability Low High Reagent Blanks 7 Day 0 Hour Point 1: Calibration 7 Day 0 Hour MB Type Factor: 1-Point Calibration Point With CONC-0 # User Defined! Do Not Use Lithium Heparin Plasma Samples * To work in SI units (meq/l) multiply by 1.0

4 , AU680 Beckman Coulter System Reagent: OSRT056 Test Name: Li < > Type: Serum Operation: Yes Sample Volume 2 μl Dilution 0 μl OD Limit Pre-Dilution Rate 10 Min. OD Max. OD Reagents Volume: R1(R1-1) 150 μl Dilution 0 μl Reagent OD limit: R2 Volume 0 μl Dilution 0 μl First Low High 0.8 Last Low High 0.8 Dynamic Range Low 0.1* High 5.0* Common Reagent Type None Name Correlation Factor A 1 B 0 Wavelength: Pri. 520 nm Sec. 480 nm Factor for Maker A 1 B 0 Method: END Reaction slope: - Onboard Stability 14 Days 0 Hour Measuring Point 1: First 0 Last 6 LIH Influence Check # Measuring First Last Lipemia Linearity: % Icterus No Lag Time: Hemolysis Range Test Name: Li < > Type: Serum Value/Flag: # Level L: # Level H: # Specific Ranges: From To Panic Value Sex Year Month Year Month Low High Low High 1. # # # # # # # # # 2. # # # # # # # 3. # # # # # # # 4. # # # # # # # 5. # # # # # # # 6. # # # # # # # 7. No demographics # # 8. Not within expected values # # Unit mmol/l* Decimal Places # Test Name: Li < > Type Serum Use Serum Cal. Calibration Type: AB Formula: Y=AX+B Counts: # <Calibrator Parameters> Slope Check None Calibrator OD Conc Factor Range Low High Allowable Range Check Point 1: # 3.0* 7.0* Reagent Blank Calibration Point 3: Advanced Calibration Point 4: Operation Yes Interval (RB/ACAL) Lot / Lot Point 8: Point 9: Point10: <Point Cal. For Master Curve> No. of Correction Points Use Master Curve Lot Calibration Calibrator OD Conc OD Range Stability Low High Reagent Blanks 7 Day 0 Hour Point 1: Calibration 7 Day 0 Hour MB Type Factor: 1-Point Calibration Point With CONC-0 # User Defined! Do Not Use Lithium Heparin Plasma Samples * To work in SI units (meq/l) multiply by 1.0

5 Beckman Coulter System Reagent, AU600 Specific test parameters Test No # Test name Li Sample type Ser Page 1/2 Sample vol. 2 Dil. vol 0 μl Min. OD Max. OD Reagent 1 vol 150 Dil. vol 0 μl L H Reagent 2 vol 0 Dil. vol 0 μl Reagent OD limit Fst. L -0.5 Fst. H 0.80 Wave Main 520 Sub 480 Lst. L -0.5 Lst. H 0.80 Method END Dynamic range Reaction - L 0.1* H 5.0* Point 1 Fst 0 Lst 6 Correlation factor A 1 Point 2 Fst Lst B 0 Linearity Fst % Sec % No lag time On-board stability period 14 Select using Space key, or select from list displayed by Guide key Test No # Test name Li Sample type Ser Page 2/2 Level L Level H Value/flag # # # Normal range Sex Age L Age H L H 1 # # Y # M # Y # M # # 2 # # Y # M # Y # M # # 3 # # Y # M # Y # M # # 4 # # Y # M # Y # M # # 5 # # Y # M # Y # M # # 6 # # Y # M # Y # M # # 7 Non select # # 8 Out of range # # L H Panic value # # Select the function using the Function key or the Mouse Calibration specific Test No # Name Li Cal type 8 AB Count # Formula 1 Y=AX+B Process Conc Selection calibrator Cal No OD Conc Factor/OD-L Factor/OD-H Point 1 # * 3.0* 7.0* Point 2 Point 3 Point 4 Point 5 Point 6 Point 7 1-point cal. point MB type factor Calibrator stability period 30 Select the function using the Function key or the Mouse # User Defined! Do not use Lithium Heparin plasma samples Beckman Coulter 1.00 mmol/l Standard included in kit * Units are mmol/l. To work in meq/l multiply by 1.0 Dilute all calibrators and samples 1:10 with distilled or deionized water

6 Beckman Coulter System Reagent, AU2700/AU5400 Test Name: Li < > Type: Serum Operation: Yes Sample: Volume 2 μl Dilution 0 μl Pre-Dilution Rate: 10 Reagents: R1 Volume 150 μl Dilution 0 μl Min OD Max OD R2 Volume 0 μl Dilution 0 μl L H Reagent OD limit: Wavelength: Pri. 520 Sec. 480 First L -0.5 First H 0.80 Method: END Last L -0.5 Last H 0.80 Reaction slope: - Dynamic Range: Measuring Point 1: First 0 Last 6 L 0.1* H 5.0* Measuring First Last Correlation Factor: Linearity: % A 1 B 0 No Lag Time: On-board stability period: 14 Test Name: Li < > Type: Serum Value/Flag: # Level L: # Level H: # Normal Ranges: Age L Age H Sex Year Month Year Month L H ο 1. # # # # # # # ο 2. # # # # # # # ο 3. # # # # # # # ο 4. # # # # # # # ο 5. # # # # # # # ο 6. # # # # # # # 7. None Selected # # 8. Out of Range L H # # Panic Value: # # Unit: mmol/l* Decimal places: # Test Name: Li < > Type Serum Calibration Type: AB Formula: Y=AX+B Counts: # Process: CONC Cal. No. OD CONC Factor/OD-L Factor/OD-H Point 1: # * 3.0* 7.0* Point 3: Point 4: 1-Point Cal. Point: With CONC-0 Slope Check None Advanced Calibration: ## MB Type Factor: Calibration Stability Period: 7 # User defined ## Lot or Lot + Bottle * Units are in mmol/l. To work in meq/l multiply by 1.0! Do not use Lithium Heparin plasma samples

7 Beckman Coulter System Reagent: OSRT056 Parameters General LIH ISE Calculated Test Range Test Name: Li < > Type: Serum Operation: Yes Sample Volume 2 μl Dilution 0 μl OD Limit Pre-Dilution Rate 10 Diluent Bottle # Min. OD Max. OD Reagents Volume: R1(R1-1) 150 μl Dilution 0 μl Reagent OD limit: R1-2 μl Dilution μl First Low -0.5 High 0.8 Last Low -0.5 High 0.8 R2 (R2-1) 0 μl Dilution 0 μl Dynamic Low 0.1* High 5.0* Common Rgt. Type None Name None Correlation Factor A 1 B 0 Wavelength: Pri. 520 nm Sec. 480 nm Factor for Maker A 1 B 0 Method: END Reaction slope: - Onboard Stability 14 Day 0 Hour Measuring Point 1: First 0 Last 6 LIH Influence Check # Measuring First Last Lipemia Linearity Limit: % Icterus Lag Time Check: Hemolysis +++++, AU5800 Parameters General LIH ISE Calculated Tests Range Test Name: Li < > Type: Serum Value/Flag: # Level Low # High # Specific Ranges: From To Sex Year Month Year Month Low High 1. # # # # # # # 2. # # # # # # # 3. # # # # # # # 4. # # # # # # # 5. # # # # # # # 6. # # # # # # # 7. Standard demographics # # 8. Not within expected values # # Panic Value Low # High # Unit mmol/l* Decimal Places # Parameters Calibrators Calibration Parameters Test Name: Li < > Type Serum Cuvette Use Serum Cal. Calibration Type: AB Formula: Y=AX+B Counts: # <Calibrator Parameters> Slope Check None Calibrator OD Conc Factor Range Low High Point 1: # 3.0* 7.0* Allowable Range Check Point 3: Reagent Blank Point 4: Calibration Advanced Calibration Operation Yes Point 8: Interval (RB/ACAL) Lot / Lot Point 9: Point10: Lot Calibration <Point Cal. For Master Curve> No. of Correction Points Use Master Curve Calibrator OD Conc OD Range Stability Low High Reagent Blank 7 Day 0 Hour Point 1: Calibration 7 Day 0 Hour MB Type Factor: 1-Point Calibration Point With Conc-0 # User Defined! Do Not Use Lithium Heparin Plasma Samples * To work in SI units (meq/l) multiply by 1.0

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