Andreas Reimann Berlin im Juli 2009

Transcription

1 Agilent Technologies ASMS 2009 Andreas Reimann Berlin im Juli 2009

2 New Triple Quad LC/MS Portfolio 6410, 6430 & 6460 Page 2

3 Comparison of 6400 Series QQQs 6410BA Sensitivity: 1 pg reserpine S/N > 150:1 S/N > 300:1 S/N > 1,000:1 1xRMS 1xRMS 1xRMS Mass Range 5 2,000 amu 5 2,250 amu 5 3,000 amu Minimum Dwell Time 5 msec 1 msec 1 msec Polarity Switching 250 msec* 30 msec 30 msec Scan Speed 5,200 u/sec 5,200 u/sec 5,200 u/sec No. of MRMs 10,000 20,000 20,000 Dynamic MRMs 4,000 4,000 4,000 Mass Resolution 0.7 Da 0.5 Da 0.5Da *New Polarity Switching Specification for the 6410BA in August Page 3 Page 3

4 The new 6430 Triple Quad: ideal QQQ for ChipCube <10 attomole sensitivity 6430 Triple Quad LC/MS HPLC Chip System Page 4

5 2,000 DMRM transitions for tryptic peptides in depleted human plasma with 6400 Series QQQ Page 5

6 Collaboration with a leader in Biomarker Research SANTA CLARA, Calif., and WASHINGTON, May 26, 2009 Agilent Technologies Inc. (NYSE: A) and Anderson Forschung Group LLC (AFG) today announced a collaboration to develop quantitative peptide assays to speed protein biomarker discovery and validation. Leigh Anderson, CEO of the Plasma Proteome Institute tute The collaboration will combine AFG s stable isotope standards and capture by anti-peptide antibodies (SISCAPA) technology with Agilent s 1200 Series HPLC- Chip and 6400 Series triple quadrupole mass spectrometers (MS) SISCAPA assays for candidate biomarkers can benefit substantially from the reproducibility and sensitivity of Agilent s platform, and we look forward to optimizing this combination, said Leigh Anderson, AFG s chief executive officer. Page 6

7 Agilent 6540 Ultra High Definition (UHD) Accurate Mass Q-TOF LC/MS Exceptional accurate mass, sensitivity, dynamic range and resolution perfect match for 1290 Infinity UHPLC Designed for high confidence analysis of complex samples

8 Ultra High Definition Optimizing all Analytical Dimensions Signal Response Sensitivity Dynamic Range Linearity Separation Speed Peak Capacity Mass Spectrum Mass Accuracy Resolving Power Acquisition Rate

9 Agilent Q-TOF Family 6530AA Q-TOF - 20K resolution - 1 pg sensitivity - 5 decades in-scan 6520AA Q-TOF AA/ 6538 Q-TOF -40K resolution/ - < 1 PPM MS, 3 PPM MS/MS -2pg sensitivity - 5 decades in-scan Shipping Oct AA Q-TOF - 20K resolution - 20K resolution - 5 Pg Sensitivity - 1 pg sensitivity - 5 decades in-scan - 5 decades in-scan 6510AA Q-TOF - 13K resolution decades in-scan - 5 Pg Sensitivity - Obsolete June BA Q-TOF - 20K resolution - 5 Pg Sensitivity - 5 decades in-scan Page 9

10 Agilent 6540 Ultra High Definition Accurate Mass Q-TOF 500 ppb mass accuracy femtogram sensitivity 5 decades dynamic range 40,000 resolving power 20 Spectra/sec Excellent Linearity and Isotopic Fidelity Supports Agilent Jet Stream and Supports Agilent Jet Stream and HPLC-Chip

11 Ion Beam Compression (IBC)* Technology Drives Higher Resolution Compressed and cooled ion beam ensures the best sensitivity performance in high resolution mode Exit from collision cell Into slicer and pulser region Narrowed beam slit enables mass resolution of 40K Up to 10-fold ion beam compression * Patent pending

12 Enhanced Mirror Technology (EMT) Enhanced Mirror Technology minimizes variation and distribution of ion arrival time and energies 2nd order time focusing Deeper electrostatic ion mirror 35% longer flight tube

13 Next Generation Ultra High Speed Detector New Bipolar TOF Detector New ultra fast and high efficiency scintillator New ultra fast response PMT design continues the tradition of high dynamic range and detector lifetime Developed by Photonis with Agilent TOF Technology Specifically enhances Resolution in 2Ghz Ext. Dynamic Range Mode 2 nsec/div Single Ion Response ~800 psec FWHM Making Research Grade Performance possible in a Benchtop Format

14 Ultra High Speed Acquisition From Agilent s Leadership in GHz Speed Electronics 4 GHz Acquisition for Maximum Resolving Power and <1ppm Mass Accuracy 5 Decades of in-spectrum Dynamic Range from 2-Channel x 2 GHz Dual Gain Mode Dual Input Agilent pre-amplifiers Picture of 4GHz board Goes here 4 GHz (8 bit) Analog-Digital-Converter Adapted from Agilent s High Speed Oscilloscope Systems Ultra High Speed FPGAs process and store transients in real time FPGAs 4 GHz Agilent ADC Making Research Grade Performance possible in a Benchtop Format

15 6540 Ultra High Definition Q-TOF Key Design Enhancements Feature Advantage Benefit Ion Beam Compressioncompresses ion beam into dense layer with uniform energy Enhanced Mirror Technologydeeper mirror with 2 nd order focusing Longer Flight Tube 35% increase in length Fast Bipolar Detector 2X faster than previous version Fine tune Internal Reference Based Calibration Minimize sensitivity loss while passing through narrow slit Minimize distribution of ion arrival time and energy Added length provides more time for ions to resolve Greater mass resolution in extended dynamic range mode 2-3 fold improvement in mass accuracy Greater mass resolution without sensitivity loss Greater mass resolution Greater mass resolution ID more compounds in complex mixtures- metabolomics Greater confidence for compound ID

16 Enhancements in Resolving Power High Resolution and Ext. Dynamic Range Modes r Resolvin ng Powe High Resolution 6540 Ext. Dynamic Range 6530 High Resolution m/z Ext. Dynamic Range

17 6540 Ultra High Definition QTOF Maintaining i i Resolving Power Across the Mass Range m/z 622 and isotopes R= x10 R R= R= R= R= R= R= Counts vs. Mass-to-Charge (m/z) R= R= Counts vs. Mass-to-Charge (m/z) Scan Rate Independent d 17 Orbitrap evaluation March 2009

18 6540 Ultra High Definition QTOF Mass Accuracy Repetitive Injections 40pg reserpine on-column, 10 injections +ESI EIC( ) Scan Frag=240.0V Reserpine_40pgms3.d Counts vs. Acquisition Time (min) Error Run (ppm) Mean 0.25 Std. Dev 0.32 x Isotope Obs % Calc % Obs m/z Calc m/z Diff (ppm) Counts vs. Mass-to-Charge (m/z) 250 ppb mass accuracy calibration and very accurate isotopic ratios

19 6540 Ultra High Definition QTOF Sensitivity Full Scan MS Mode- 1 picogram 4 x10 S/N = 319 RMS Excellent sensitivity, accuracy and resolution at 1 pg on column 3 x10 2 (M+H) Ratio m/z Diff. 2 Resolution ~ 33,000 Theor. Expt. Theor. Expt. (ppm) Counts vs. Acquisition Time (min) Counts vs. Mass-to-Charge (m/z)

20 6540 Ultra High Definition QTOF Peak Wide Dynamic Range G2421A Tune Mix m/z sec spectrum Dynamic Range: 4 5,000, Peak height 5,000,000 x = 50, x x Height Co x Counts vs. Mass-to-Charge (m/z) Counts vs. Mass-to-Charge (m/z) Counts vs. Mass-to-Charge (m/z) +ESI Scan (#27) Frag=180.0V 2GHz_accF.d

21 1290/ sec analysis 250 pesticides (125pg on-column) 4 x naphthol Mass [M+H] + = Resolution = ppm x Cpd 201: Naphthol, Counts vs. Mass-to-Charge (m/z) x Spinosad A Mass [M+H] + = Resolution = ppm Counts vs. Mass-to-Charge (m/z) 4 x Fenpyroximate E Mass [M+H] + = Resolution = ppm Counts vs. Mass-to-Charge (m/z) Counts vs. Acquisition Time (sec)

22 Horse Heart Myoglobin (MW Da) 6540 QTOF, High Res Mode Average resolution of these peaks R = Heme Intact Proteins on 6540 QTOF April 2009

23 3 4 Chicken Lysozyme (MW Da) 6540 QTOF, High Res Mode R ~ R ~ R ~ Intact Proteins on 6540 QTOF April 2009

24 What is the Value of Ultra High Definition Q-TOF Coupled with 1290 Infinity UHPLC? Less ion suppression Find more trace level compounds Reduce number of false positives Higher confidence in molecular formula generation More confidence in compound ID Faster analysis greater productivity Higher peptide sequence coverage Greater sensitivity

25 Improving Data Quality- Faster is Better! Take advantage of peak capacity to find more trace level compounds in complex mixtures Take advantage of peak capacity to reduce ion suppression from interfering co-eluting matrix ions Take advantage of high speed acquisition to increase data g g p q sampling for better mass accuracy and mass resolution