Large scale SNP data management and integration in breeding programs
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1 Large scale SNP data management and integration in breeding programs Eildert Groeneveld Institute of Farm Animal Genetics
2 TheSNPpit: Who is it for? Needs to manage many SNPs Uses standardized workflows Uses pipelines Does some gblup Eildert Groeneveld Interbull Tallinn August 26/27,
3 TheSNPpit Genotyping labs import SNPpit database export -E gs_055 data use/analysis chicken maf.05 plink -E gs_022 pigs genable -E gs_123 beef GS run week12 Define Subsets: Maf Nocalls Chrom Ind SNP -E gs_086 -E gs_009 -E gs_031 dairy HF gblup dairy chrom 12 GWAS goat imp 54-> 850K 3 / 32
4 Genotype sets SNP vector Sample vector Eildert Groeneveld Interbull Tallinn August 26/27,
5 Genotype sets SNP vector Sample vector Eildert Groeneveld Interbull Tallinn August 26/27,
6 Genotype sets Eildert Groeneveld Interbull Tallinn August 26/27,
7 TheSNPpit command line Map info for each chip >snppit --import panel ctl_800k 800k.map >snppit --import panel shp_57k shp_57k.map Done once 7 / 32
8 TheSNPpit command line Import SNP data snppit -import data ctl_800k wk13.ped snppit --import data ctl_800k wk14.ped snppit --import data shp_57k wk snppit --export gs_012 snppit -export gs_023 Export SNP data 8 / 32
9 Breeding prg: workflow comp Phenotype database SNPpit database 9 / 32
10 Breeding prg: workflow Phenotype database Extract pedigree Extract phenotype data Extract list of ID with SNP data Extract list of SNPname/panel SNPpit database Provide SNP data for ID/SNPs Run gblup 10 / 32
11 Genotype sets - reminder Eildert Groeneveld Interbull Tallinn August 26/27,
12 Breeding prg: workflow cc Phenotype database On your table: wk21_anis.txt, wk21_snps.txt, pheno.dat,pedi.dat SNPpit database 12 / 32
13 Breeding prg: workflow cc Phenotype database On your table: wk21_anis.txt, wk21_snps.txt, pheno.dat,pedi.dat SNPpit database 13 / 32
14 Breeding prg: workflow cc Phenotype database On your table: wk21_anis.txt, wk21_snps.txt, pheno.dat,pedi.dat SNPpit database >snppit --create ind_sel -p ctl_800 -i wk21_anis.txt > is_ / 32
15 Breeding prg: workflow cc Phenotype database On your table: wk21_anis.txt, wk21_snps.txt, pheno.dat,pedi.dat SNPpit database >snppit --create ind_sel -p ctl_800 -i wk21_anis.txt > is_123 >snppit --create snp_sel -p ctl_800 -i wk21_snps.txt > ss_ / 32
16 Breeding prg: workflow cc Phenotype database On your table: wk21_anis.txt, wk21_snps.txt, pheno.dat,pedi.dat SNPpit database >snppit --create ind_sel -p ctl_800 -i wk21_anis.txt > is_123 >snppit --create snp_sel -p ctl_800 -i wk21_snps.txt > ss_243 >snppit --create genotype_set is_123 ss_243 > gs_ / 32
17 Breeding prg: workflow cc Phenotype database On your table: wk21_anis.txt, wk21_snps.txt, pheno.dat,pedi.dat SNPpit database >snppit --create ind_sel -p ctl_800 -i wk21_anis.txt > is_123 >snppit --create snp_sel -p ctl_800 -i wk21_snps.txt > ss_243 >snppit --create genotype_set is_123 ss_243 > gs_434 >snppit -export gs_434 -o SNPs_wk >gblup_prg pheno.dat pedi.dat SNPs_wk / 32
18 Breeding prg: workflow script Put the following in file run_newexport.sh : #!/bin/bash IS=$(snppit -q -C individual_selection -p 57K -i $1_ANIs.txt) SS=$(snppit -q -C snp_selection -p 57K -i $1_SNPs.txt) GS=$(snppit -q -C genotype_set --snp $SS --individual $IS echo ' new genotype set: '$GS snppit -E genotype_set --name $GS -f o $ Run as: >bash run_newexport.sh wk21 18 / 32
19 Breeding prg: workflow timings Assumptions animals with SNPs > wk21_anis.txt SNP from 57K panel > wk21_snps.txt 19 / 32
20 Breeding prg: workflow timings Assumptions animals with SNPs > wk21_anis.txt SNP from 57K panel > wk21_snps.txt SNPpit database >snppit --create ind_sel -p ctl_57 -i wk21_anis.txt :.57sec 20 / 32
21 Breeding prg: workflow timings Assumptions animals with SNPs > wk21_anis.txt SNP from 57K panel > wk21_snps.txt SNPpit database >snppit --create ind_sel -p ctl_57 -i wk21_anis.txt : >snppit --create snp_sel -p ctl_57 -i wk21_snps.txt :.57sec.56sec 21 / 32
22 Breeding prg: workflow timings Assumptions animals with SNPs > wk21_anis.txt SNP from 57K panel > wk21_snps.txt SNPpit database >snppit --create ind_sel -p ctl_57 -i wk21_anis.txt : >snppit --create snp_sel -p ctl_57 -i wk21_snps.txt :.57sec.56sec >snppit --create genotype_set is_123 ss_243 :.28sec 22 / 32
23 Breeding prg: workflow timings Assumptions animals with SNPs > wk21_anis.txt SNP from 57K panel > wk21_snps.txt SNPpit database >snppit --create ind_sel -p ctl_57 -i wk21_anis.txt : >snppit --create snp_sel -p ctl_57 -i wk21_snps.txt :.57sec.56sec >snppit --create genotype_set is_123 ss_243 :.28sec >snppit -export gs_434 -o SNPs_wk : 51.07sec 23 / 32
24 Breeding prg: workflow timings Assumptions animals with SNPs > wk21_anis.txt SNP from 57K panel > wk21_snps.txt SNPpit database >snppit --create ind_sel -p ctl_57 -i wk21_anis.txt : >snppit --create snp_sel -p ctl_57 -i wk21_snps.txt :.57sec.56sec >snppit --create genotype_set is_123 ss_243 :.28sec >snppit -export gs_434 -o SNPs_wk >gblup_prg pheno.dat pedi.dat SNPs_wk : 51.07sec :?????sec 24 / 32
25 Scaling: Storage 2 bits/snp - 4 SNP/byte 4096 mio SNP / 1 MB Eildert Groeneveld Interbull Tallinn August 26/27,
26 Scaling: Storage 2 bits/snp - 4 SNP/byte 4096 mio SNP / 1 MB Eildert Groeneveld Interbull Tallinn August 26/27,
27 Scaling: Storage 2 bits/snp - 4 SNP/byte 4096 mio SNP / 1 MB Eildert Groeneveld Interbull Tallinn August 26/27,
28 Ressources SNPpit server Linux TheSNPpit software User Manual 28 / 32
29 Ressources SNPpit server Linux TheSNPpit software 29 / 32
30 Ressources SNPpit server Linux TheSNPpit software Open Source Open Source 30 / 32
31 Eildert Groeneveld Interbull Tallinn August 26/27,
32 Thank you for your attention Eildert Groeneveld Interbull Tallinn August 26/27,
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