Doc. No: D Rev. 01 Last revision: TEST1 ROLLER 20 ROLLER 10/10PN ROLLER 20PN INTERFACING MANUAL

Transcription

1 Doc. No: D Rev. 01 Last revision: TEST1 ROLLER 20 ROLLER 10/10PN ROLLER 20PN INTERFACING MANUAL

2 REVISIONS Revision n 00 08/07/ /07/200 8 Date Author Software TEST1 Software ROLLER2 0 Software ROLLER10/20P N Comments AP 4.05x 5.02 Creation AP 601F 6.01C R P ROLLER10/20PN INTEGRATION INTERFACING LATEX PROTOCOL CONTACT ADDRESS MANUFACTURER SIRE Analytical Systems S.r.l. Via Biella 121/ UDINE ITALY tel / fax / WORLDWIDE EXCLUSIVE DISTRIBUTOR ALIFAX s.p.a. Alifax S.p.A. via Petrarca, 2/ Polverara (PD) - Italy Tel Fax

3 INDEX 1. INTRODUCTION ADD PARTS to INSTALL USEFUL TOOLS FOR DEBUGGING SYMBOLS TEST1 RS232 PORTS features: SERIAL CABLE PIN CONNECTIONS TO CONNECT TEST1 TO PC-LAB: TEST1 protocol options RS232 test function to check the communication of TEST QUERY PROTOCOL project for TEST Protocol description: ROLLER20 (old model) RS232 PORTS features: SERIAL CABLE PIN CONNECTIONS TO CONNECT ROLLER20 TO PC-LAB: ROLLER 20 protocol options RS232 test function to check the communication of ROLLER20 (OLD MODEL) QUERY PROTOCOL project for ROLLER Protocol description: ROLLER 10-10PN - 20PN RS232 PORTS features: SERIAL CABLE PIN CONNECTIONS TO CONNECT ROLLER10PN/20PN TO PC-LAB: ROLLER10PN/20PN PROTOCOL OPTIONS: RS232 test to check the communication of ROLLER10PN/20PN QUERY PROTOCOL project for ROLLER 10-10PN / ROLLER20PN Protocol explanation (BCI Configuration): TEST1, ROLLER10PN-ROLLER20PN and ROLLER20(old model) Multiple connections INTERFACING LATEX PROTOCOL LATEX PROTOCOL INTERFACE EXPLICATION Up to Sw. version 6.01C LATEX PROTOCOL INTERFACE EXPLICATION From Sw. version 6.01D (Test 1 and new Roller 10-10PN / 20PN) Note: The paragraphs written with the italic blue characters (as on this note) indicate a variation or an addition to the previous version of the manual.

4 1. INTRODUCTION This manual is focused to give detailed information about serial ports connections available on TEST1 ROLLER20 (old model) ROLLER10/10PN/20PN instruments and communication protocol features. It should help Software House Companies during the modification or the installation of their program in the Host Computer. COMMON PARTS 2. ADD PARTS to INSTALL A standard cable to connect two Personal Computers via serial port. This cable must have: D-type 25 pin female connector at one side and a D-type 9 pin female connector at the opposite side for TEST1 and ROLLER20 (OLD MODEL). D-type 9 pin female connector at one side and a D-type 9 pin female connector at the opposite side for ROLLER10PN/20PN. 3. USEFUL TOOLS FOR DEBUGGING A PC computer with a communication program installed (like HyperTerminal of Windows) to test the instrument communications. HyperTerminal allows the technician to check the communication messages byte by byte. To run it follow the path: start, all programs, accessories, communication, HyperTerminal; run it and choose new connection. Type a preferred name e.g. TEST1, press OK and then EXIT. Open the curtain menu pressing File on the left upper side of HyperTerminal window and Property; a new window will be opened. Press Connection and choose the serial port where the serial cable has been connected: e.g.com1. Press Configuration and choose the speed which should be 9600Bps. Press OK twice. 4. SYMBOLS The below symbols will be displayed on HyperTerminal if the program is configured with Terminal character. = STX symbol = ETX symbol = ACK symbol = NAK symbol CHECKSUM = XOR calculation of part of R received message to check the correctness of it. 1

5 5. TEST1 RS232 PORTS features: Communication ports parameters are fixed and cannot be modified. Baud rate 9600 Parity N Data bit number 8 Stop bit 1 HyperTerminal program must have the same parameters to a correct data exchanging. PORT2 connection. This instrument communicates through Serial Port 2. This port uses a 25 pin male (DB25) serial connector. The used pins are: no. 2 for TXDATA, no. 3 for RXDATA and 7 for ground. The remaining pins are connected together as nullmodem configuration (4-5-8 and 6-20). Serial Port 1 cannot be used for communication. It is connected internally to the INTERNAL BAR CODE READER (IBCR if installed) or it can be connected to an EXTERNAL BAR CODE READER (EBCR) The maximum number of ASCII characters for each patient ID is 15 (fifteen). PORT1 connection 6. SERIAL CABLE PIN CONNECTIONS TO CONNECT TEST1 TO PC-LAB: DB9 F DB25 F 2

6 7. TEST1 protocol options The right choice of the protocol enables the Query functionality. The table below specify different configurations: PROTOCOL Query DESCRIPTION R message structure SYMBOLS NOTES DAT8 No Patient ID codes, STX 1 byte Checksum note having length of R 1 byte characters of 8 digits at Not used 2 byte maximum, could Identification no 8 byte be read by an Not used 6 byte external bar code ESR result 4 byte reader (EBCR) or Checksum 1 byte they could be introduced typing ETX 1 byte them manually. The output message structure will be followed by the sedimentation curve date (like STD) if KINET option in the COMMUNICA- TION menu is enabled. TOTAL = 24 byte Checksum value is the result of Exclusive OR (XOR) of all characters of the message, excluded STX, ETX and Checksum. If the Checksum value calculated by the analyzer is 03 (corresponding to ETX), or 02 (corresponding to STX), or 00 (corresponding to NULL), the value will be automatically replaced with 7F hex value. Software House must adopt the same procedure when calculating checksum of the strings received from the analyzer. PROTOCOL Query DESCRIPTION R message structure SYMBOLS NOTES DAT15 Yes It works by an STX 1 byte internal (IBCR) R 1 byte or external bar code reader Workstation number 2 byte (EBCR). It Identification no 15 byte accepts the Rack no 2 byte maximum Position 2 byte patient identity Cycle/Bayer (Note) 2 byte code with 15 ESR result 4 byte characters of Checksum 1 byte length. This ETX 1 byte protocol will be activated automatically if an IBCR is connected. TOTAL = 31 byte Checksum note Checksum value is the result of Exclusive OR (XOR) of all characters of the message, excluded STX, ETX and Checksum. If the Checksum value calculated by the analyzer is 03 (corresponding to ETX), or 02 (corresponding to STX), or 00 (corresponding to NULL), the value will be automatically replaced with 7F hex value. Software House must adopt the same procedure when calculating checksum of the strings received from the analyzer. Bayer note: This field is managed by Bayer parameter on tech/communication Menu: If Bayer is 0, the field deals with the number of Cycle otherwise if Bayer is 1, the field will contain '01' (hex 30 and 31) to maintain the Bayer protocol compatibility. DAT8 either DAT15 option can be choice accessing in the COMMUNICATION MENU passing through TECH MENU. Press key 5 from MAIN MENU and keys 1 and subsequently 9 as password and then key 3. If the internal bar code reader is installed in a TEST1 instrument, DAT15 will be forced automatically at powering on. In the TEST1 COMMUNICATION MENU, RS232 Protocol option can be recalled pressing key 1- DAT08 2- DAT15 This message will be displayed Option (1) Option (2) Enables the patient identification number length to 8 bytes. Enables the patient identification number length to15 bytes. 3

7 8. RS232 test function to check the communication of TEST1 It simulates one transmission of 10 TEST1 results to host computer through RS232 serial port (SERIAL PORT 2). If DAT8 is chosen, a message with 8 ID digits will be sent to the Host. Selecting DAT15, the ID digit number to send to the Host is going to be 15. DAT8 test transmission and its explanation: Field name Symbol Hex value Length Description STX 02 1 char The first field is the same to all ten strings R 52 1 char The second field is the same to all ten strings Workstation The third field is the same to all ten strings 2 char Patient ID The furth field is different from the others strings 8 char Rack no. 2 char The fifth field is the same to all ten strings Position 2 char The sixth field is different from the others Bayer (Note2) 2 char The seventh field is the same to all ten strings Result 4 char The eighth field is different from the others Checksum (Note1) 1 char The nighth field is different from the others. ETX 03 1 char The tenth field is the same to all ten strings. Note : what above explained refers to the TEST Strings instrument send to host computer, this string is displayed above this table. Note1: Checksum note Checksum value is the result of Exclusive OR (XOR) of all characters of the message, excluded STX, ETX and Checksum. If the Checksum value calculated by the analyzer is 03 (corresponding to ETX), or 02 (corresponding to STX), or 00 (corresponding to NULL), the value will be automatically replaced with 7F hex value. Software House must adopt the same procedure when calculating checksum of the strings received from the analyzer. Total length 24 char Note2: Bayer field remains activated to, '01' (hex 30 and 31) because it is only a test. DAT15 test transmission and its explanation: Field name Symbol Hex value Length Description STX ( 02 1 char The first field is the same to all ten strings R 52 1 char The second field is the same to all ten strings Workstation The third field is the same to all ten strings 2 char Patient ID The furth field is different from the others strings 15 char Rack no. 2 char The fifth field is the same to all ten strings Position 2 char The sixth field is different from the others Bayer (Note2) 2 char The seventh field is the same to all ten strings Result 4 char The eighth field is different from the others Checksum The nighth field is different from the others. 1 char (Note1) ETX 03 1 char The tenth field is the same to all ten strings. Total length 31 char Note : what above explained refers to the TEST Strings instrument send to host computer, this string is displayed above this table. Note1: Checksum value is the result of Exclusive OR (XOR) of all characters of the message, excluded STX, ETX and Checksum. If the Checksum value calculated by the analyzer is 03 (corresponding to ETX), or 02 (corresponding to STX), or 00 (corresponding to NULL), the value will be automatically replaced with 7F hex value. Software House must adopt the same procedure when calculating checksum of the strings received from the analyzer. Note2: Bayer field remains activated to, '01' (hex 30 and 31) 4

8 In the COMMUNICATION MENU, RS232 function can be recalled pressing. COMMUNICATION RS232 TEST TX This message will be displayed 9. QUERY PROTOCOL project for TEST1 This document describes the serial protocol (Query Protocol) used by TEST1 with the Internal Bar Code Reader (IBCR). It queries HOST Computer through Serial Port 2 (RS232C). This protocol is the same of QUERY TEST1 program; a Windows software designed to be loaded in a Personal Computer (LAB PC) installed in a laboratory and connected to HOST through a network. QUERY protocol uses two files to exchange data for the communication with HOST by using the LAB PC like a bridge between TEST1 and HOST Computer. Further information of QUERY TEST1 program software can be taken from the QUERY TEST1 installation and using manual. TEST1 Settings for a correct communication COMMUNICATION MENU in the TECH MENU, allows the configuration of some parameters for the correct communication protocol. The accessing in it is allowed by typing key 5 from MAIN MENU, keys 1 and subsequently 9 for the password requesting and key 3. COMMUNICATION 1. RS232 PROTOCOL 2. RS232 TEST 3. KINET. SETUP 4. SETUP [ * ] 5. ENABLE BAYER 6. MEASURE MISSING ID 7. TIME OUT UART 8. TEST1 NUMBER 9. CLEAR 5

9 The configuration for a correct Query protocol functioning is: RS232 PROTOCOL= It has to be set to DAT15 (Working with an Internal Bar Code Reader it is forced automatically). To access inside and select DAT15 press key 1 in the COMMUNICATION menu. KINET. SETUP= It must be kept to 0. Press key 3 in the COMMUNICATION menu to access inside and select 0. This parameter, when activated, sends a kinetic curve data for factory checking after having transmitted R message. SETUP [ * ]= ENABLE BAYER MEASURE MISSING ID= TIME OUT UART= Press key 4 in the COMMUNICATION menu to access inside the option and 1 to enable the asterisk character transmission to the host. It works when a analysed sample gives a low value of hematocrit (around 20% and less). The position of it in the message is explained in the Warnings (next page). Press key 5 in the COMMUNICATION menu to access Bayer option and 1 to enable it to guarantee the Bayer protocol specifications (field Cycle/Bayer = 01). If Bayer protocol is not requested this option must be kept to 0 (disable). Press key 6 in the COMMUNICATION menu to access to the option and key 1 to allow the analysis of samples without any identification number or not readable by IBCR. Keeping it to 0, these samples won t be analysed. Press key 7 in the COMMUNICATION menu to access this option to set the waiting time (from 2 to 99 seconds) if the increasing of the waiting time to receive T message and the confirmation byte (ACK) for having received R message is requested. The maximum time to receiving T message and the confirmation byte (ACK) for having received R message remain set to 2 seconds keeping it to OFF. If within this time no message comes in, the instrument will go ahead. This option works if ACK option, available pressing 9 in the COMMUNICATION menu is enabled. Verify of the parameters setting in the flag list printing: Pressing 6 in the TECH MENU, the flag list printing is going to be obtained like in the following example: TEST1 UK ver. 6.01F Jun xx:xx:xx. TEST1 SN. xxx # AVAILABILITY xxxx # GLOBAL COUNT. xxxx BoosterY Model Fact MIXING CYCLES 180 MIXING SPEED 1 Smart warning 2000 Instrument number N 1 RS232 DAT15 FLOW CONT. ON OFFSET LEV. FLOW 556 T FS fact Miss. ID set. 1 Change ID 0 ACK set 0 Print setup 1 Magnif Set 1 Calib. setup 1 Bayer. setup 1 [ * ] setup 0 Kinet.setup 0 Mixer speed 1 Offset car. 0 Offset nee. 0 Washing time 20 Vs. 0 TimeOut 20 6

10 10. Protocol description: 1. The internal Scanner reads the bar code (patient ID) from the label stuck on the tube, the instrument creates Q message and sends it to HOST computer through RS232 serial port (or to PC LAB PC if it is connected like a bridge). <STX>Q HOST computer verifies if the patient identity code is present in the working list and, accordingly, it replies with "T" message. TEST1 waits to receiving it within two seconds and then it goes ahead. If ACK option is off, TEST1 is going to remain waiting up to 2 seconds for receiving it, otherwise if it is on, TEST1 is going to wait for all the set time (TIMEOUT from 2 to 99 seconds). TIMEOUT UART time and ACK are selectable in the COMMUNICATION MENU. T message is going to have the result field filled with 0001, if the sample requires the analysis. The result field will be filled with 0000 if the sample doesn t require the analysis. <STX>T <STX>T e<ETX> 0000d<ETX> 3. After measuring (24 seconds about), TEST1 sends R message to host replacing the result field value (0001) with the ESR read value which should be between 0002 and 0120 or negative. Errors messages are reported like: -001 for SM (Sample Missing) error, -002 for NR (Not Readable) error and -004 for NF (No flow) error. <STX>R <STX>R f <ETX> l <ETX> 4. Host computer should send ACK confirmation byte ( symbol displayed on the HyperTerminal program) to TEST1 for having accepted R message. TEST1 will ignore it, if ACK is off otherwise it will wait for all the set time (TIMEOUT from 2 to 99 seconds) if ACK is OFF. ACK = Hexadecimal code 06 Warnings: 1 If a patient identity (ID) read by the TEST1 Scanner doesn t find its own correspondent code in the Host computer working list, T message will be replaced by <NAK> message ( symbol displayed on the HyperTerminal program). The sample will be however analysed and R message sent to host. (NAK Message) Hex code 21 2 If a particular sample has a very low hematocrit (around 20% and less) and * option in the communication menu is enabled, the result field of R message, sent to the host, will contains an asterisk set in the first position on the left and its own ESR result (i.e. <STX>R *015[ <ETX> If the analysis of a particular sample generates an error and * option in the communication menu is enabled, ESR result field will contain 0 in the first left position, - (minus) in the second and the error code (i.e. <STX>R \ <ETX> 3 If the internal Scanner cannot read the patient ID and MISS ID option in the Communication setup is 0, Q message won t be created. The sample without its own patient identity won t be processed. 7

11 4 If the internal Scanner cannot read the patient ID and MISS ID option in the Communication setup is 1, Q message won t be created. The sample without its own patient identity will be, however, processed and R message sent to host with an identity code generated by the instrument itself. The new patient ID, in this case, will be composed by 10 characters: cycle number (from 01 to 99) (2 characters), instrument s/n (4 characters), rack number (from 01 to 04-2 characters) sample position in the rack (from 01 to 15-2 characters). Example: Instrument s/n Rack number Cycle number Sample position in the rack A complete R string for the transmission could be: [Workstation number] [Instrument s/n] [Rack number] [ESR] [Checksum] <STX>R f <ETX> [Cycle number] [Sample position] [Cycle number (Note 2)] 8

12 11. ROLLER20 (old model) RS232 PORTS features: Communication ports parameters are fixed and cannot be modified. Baud rate 9600 Parity N Data bit number 8 Stop bit 1 HyperTerminal program must have the same parameters to a correct data exchanging. PORT2 connection. This instrument communicates to host through Serial Port 2.This port uses a 25 pin male (DB25) serial connector and the used pins are: no. 2 for TXDATA, no. 3 for RXDATA and 7 for ground. The remaining pins are connected together as null-modem configuration (4-5-8 and 6-20). PORT1 connection. Serial Port 1 has been reserved for the connection of an external SCANNER The maximum number of ASCII characters for each patient ID is 15 (fifteen). 12. SERIAL CABLE PIN CONNECTIONS TO CONNECT ROLLER20 TO PC-LAB: DB9 F DB25 F

13 13. ROLLER 20 protocol options The right choice of the protocol enables the Query functionality. The table below specify the different configurations: PROTOCOL Query DESCRIPTION R message structure SYMBOLS NOTES BCI Yes It works by an STX 1 byte internal (IBCR) or R 1 byte external bar code Workstation number reader (EBCR). It 2 byte accepts up to 15 Identification n 15 byte characters of maximum patient Rack number 2 byte identity code. The Position 2 byte instrument enables Cycle/Bayer 2 byte Query protocol which ESR result 4 byte enquires the host if a Checksum 1 byte specific sample ETX 1 byte needs to be TOTAL = 31 byte analyzed or not. STD, DAT8 DAT15 or BCI protocol can be selected by software accessing in the SETUP menu. Checksum note: Checksum value is the result of Exclusive OR (XOR) of all characters of the message, excluded STX, ETX and Checksum. If the Checksum value calculated by the analyzer is 03 (corresponding to ETX), or 02 (corresponding to STX), or 00 (corresponding to NULL), the value will be automatically replaced with 7F hex value. Software House must adopt the same procedure when calculating checksum of the strings received from the analyzer. Bayer note: This field is managed by Bayer parameter on Tech Menu. If Bayer is 0, the field deals with the number of Cycle otherwise if Bayer is 1, the field will contain '01' (hex 30 and 31) to maintain the Bayer protocol compatibility. In the SETUP MENU list, RS232 Protocol can be recalled by pressing or until the following message is displayed: SETUP RS232 PROTOCOL Confirm the function by pressing The following menu will be displayed: 1 - STD 2 - DAT DAT15 4 BCI _ 10

14 14. RS232 test function to check the communication of ROLLER20 (OLD MODEL) It simulates one test transmission of 10 test results to host computer through RS232 serial port (SERIAL PORT 2). If DAT8 is chosen, a message with 8 ID digits will be sent to the Host. Selecting DAT15 or BCI, the ID digit number to send to the Host is going to be 15. STD test transmission and its examination: No test is available in STD test DAT8 test transmission and its examination: Field name Symbol Hex value Length Description STX 02 1 char The first field is the same to all ten strings R 52 1 char The second field is the same to all ten strings Workstation The third field is the same to all ten strings 2 char Patient ID The furth field is different from the others strings 8 char Rack no. 2 char The fifth field is the same to all ten strings Position 2 char The sixth field is different from the others Bayer (Note 2) 2 char The seventh field is the same to all ten strings Result 4 char The eighth field is different from the others Checksum (Note 1) 1 char The nighth field is different from the others. ETX 03 1 char The tenth field is the same to all ten strings. Total length 24 char Note : what above explained refers to the TEST Strings instrument send to host computer, this string is displayed above this table. Checksum note: Checksum value is the result of Exclusive OR (XOR) of all characters of the message, excluded STX, ETX and Checksum. If the Checksum value calculated by the analyzer is 03 (corresponding to ETX), or 02 (corresponding to STX), or 00 (corresponding to NULL), the value will be automatically replaced with 7F hex value. Software House must adopt the same procedure when calculating checksum of the strings received from the analyzer. Bayer note: Bayer field remains activated to, '01' (hex 30 and 31) because it is only a test. DAT15 test transmission and its examination: Field name Symbol Hex value Length Description STX 02 1 char The first field is the same to all ten strings R 52 1 char The second field is the same to all ten strings Workstation The third field is the same to all ten strings 2 char Patient ID The furth field is different from the others strings 15 char Rack no. 2 char The fifth field is the same to all ten strings Position 2 char The sixth field is different from the others Bayer (Note 2) 2 char The seventh field is the same to all ten strings Result 4 char The eighth field is different from the others Checksum (Note 1) 1 char The nighth field is different from the others. ETX 03 1 char The tenth field is the same to all ten strings. Total length 31 char Note : what above explained refers to the TEST Strings instrument send to host computer, this string is displayed above this table. Checksum note: Checksum value is the result of Exclusive OR (XOR) of all characters of the message, excluded STX, ETX and Checksum. If the Checksum value calculated by the analyzer is 03 (corresponding to ETX), or 02 (corresponding to STX), or 00 (corresponding to NULL), the value will be automatically replaced with 7F hex value. Software House must adopt the same procedure when calculating checksum of the strings received from the analyzer. Bayer note: Bayer field remains activated to, '01' (hex 30 and 31) because it is only a test. 11

15 BCI test transmission and its examination: Field name Symbol Hex value Length Description STX 02 1 char The first field is the same to all ten strings R 52 1 char The second field is the same to all ten strings Workstation The third field is the same to all ten strings 2 char Patient ID The furth field is different from the others strings 15 char Rack no. 2 char The fifth field is the same to all ten strings Position 2 char The sixth field is different from the others Bayer (Note 2) 2 char The seventh field is the same to all ten strings Result 4 char The eighth field is different from the others Checksum (Note 1) 1 char The nighth field is different from the others. ETX 03 1 char The tenth field is the same to all ten strings. Total length 31 char Note : what above explained refers to the TEST Strings instrument send to host computer, this string is displayed above this table. Checksum note: Checksum value is the result of Exclusive OR (XOR) of all characters of the message, excluded STX, ETX and Checksum. If the Checksum value calculated by the analyzer is 03 (corresponding to ETX), or 02 (corresponding to STX), or 00 (corresponding to NULL), the value will be automatically replaced with 7F hex value. Software House must adopt the same procedure when calculating checksum of the strings received from the analyzer. Bayer note: Bayer field remains activated to, '01' (hex 30 and 31) because it is only a test. In the TECH MENU, RS232 function can be recalled pressing or for many times until the following message is displayed. COMMUNICATION RS232 TEST TX Press to run the test 12

16 15. QUERY PROTOCOL project for ROLLER20. This paragraph describes the serial protocol (Query Protocol) used by ROLLER20 with an External Bar Code Reader (EBCR) connected to Serial Port 2 (RS232C). This protocol is the same used by QUERY TEST1 program; a Windows software designed to be loaded in a Personal Computer (LAB PC) installed in a laboratory and connected to HOST through a network. QUERY protocol uses two files to exchange data for the communication with HOST by using the LAB PC like a bridge between ROLLER20 and HOST Computer. Further information of QUERY TEST1 program software can be taken from the QUERY TEST1 installation and using manual. Settings for a Query protocol correct communication on ROLLER20 The configuration for a correct Query protocol functioning is: RS232 PROTOCOL= It must be set to BCI (And an External Bar Code Reader must be installed). This function is available in the SETUP MENU. It can be recalled by pressing or until the following message is displayed: SETUP RS232 PROTOCOL Confirm the function by pressing The following menu will be displayed: 1 - STD 2 - DAT DAT15 4 BCI _ Make your choice and press CLEAR twice to go back MAIN MENU Chose 4 for a Query correct communication: 13

17 KINET. SETUP= This function is available in the SETUP MENU. It can be recalled by pressing or until the following message is displayed: SETUP KINET. SETUP Confirm the function by pressing The following message will be displayed: KINET. SETUP= 0 1 -Y 0-N _ It must be set to 0 SETUP [ * ]= This function is available in the TECH MENU. It can be recalled by pressing or until the following message is displayed: TEC. CAL MENU 19 [ * ] SETUP Confirm the function by pressing The following message will be displayed: 1- [ * ] [ Y ] 0- [ * ] [ N ] The asterisk function activated allows the instrument to send the asterisk parameter to the Host computer through the serial port. It will be displayed near the ESR value if the sample analyzed has a haematocrit value low (< 20%). IDENTITY ESR *045 Example of ESR result (045) with the * parameter enabled and sent to the host computer with the patient identification together. IDENTITY ESR Example of a sample analysed with a haematocrit value over 20% and * parameter enabled. The ESR result (5) will be sent to the host computer using this form. Note: Having disabled the asterisk function, the instrument cannot send the asterisk character to the host if the analysed sample had a haematocrit value lower then 20% but it will be printed out next the ESR result however. 14

18 ENABLE BAYER Bayer communication protocol detects the Bayer field value (the previous field of the ESR result) in the R message. If Bayer function in the COMMUNICATION menu is enabled, the field will contain '01' (hex 30 and 31) to maintain the Bayer protocol compatibility otherwise if it is disabled, the field will report the number of Cycle. This function is available in the TECH MENU. It can be recalled by pressing or until the following message is displayed: TEC. CAL MENU 7 ENABLE BAYER Confirm the function by pressing The following message will be displayed: 1- ENABLE [Y] 0- DISABLE [N] Query setup= This function is available in the TECH MENU. It can be recalled by pressing or until the following message is displayed: TEC. CAL MENU 17 QUERY SETUP Confirm the function by pressing The following message will be displayed: 1- Query [ Y ] 0- Query [ N ] If Query is enabled (default), the instrument will be able to interrogate the Host computer for the analysis requesting of each specific sample. ACK= This function is available in the TECH MENU. It can be recalled by pressing or until the following message is displayed: TEC.CAL MENU 18 ACK SETUP Confirm the function by pressing The following message will be displayed: 1 ACK [Y] 0 ACK [N] This function allows to extend the waiting time for receiving T message (Query answer from the HOST computer) and ACK (acknowledge message confirmation from the HOST computer for having received R message). To modify the extension time (in seconds), it is available the TIMEOUT function explained in the Protocol description (Paragraph 15). 15

19 Parameters verification: In the SETUP menu you will find FLAG LIST function. Press or until the following message is displayed: FLAGS SETUP LIST Confirm the function by pressing Below is an example ROLLER 20 UK ver. 6.01C ROLLER20 SN. xxx # AVAILABILITY xxxx # GLOBAL COUNT. xxxx GAIN MIXING CYCLES 180 MIXING SPEED 1 RS232 BCI FLOW CONT. ON OFFSET LEV. FLOW 800 OT OFFSET LEV. 700 T ROLLER n. 1 ACK setup 0 Query setup 1 Print setup 1 Calib. setup 1 Bayer. setup 1 [ * ] setup 0 Kinet.setup 0 Offset nee. 0 Vs 0 TimeOut 20 Warning SMART

20 16. Protocol description: 1. The patient bar code ID stuck on each sample tube can be read by using of an External Bar Code Reader connected at <STX>Q the ROLLER Serial Port1. The instrument will create and send Q message to the HOST computer through RS232 serial port (or to LAB PC if used like a bridge) if Query Setup option is 1. (Note 1) 2. HOST computer will verify if the patient identity has been inserted in the working list and then it replies with "T" message. If ACK option (in the TECH MENU list) is 0, ROLLER 20 remains waiting up to 2 seconds for receiving it. otherwise if ACK option is 1 it will wait for all the set time (TIMEOUT from 2 to 99 seconds). TIMEOUT UART time option is located in the TECH MENU list. (Note 2) As soon as the instrument receives T message, it will be checked. The wheel will rotate to one position to allow the operator to insert the sample tube, if T message has the result field <STX>T compiled with 0001 (that means the sample requires the analysis). The wheel remains stopped, if the result field will be <STX>T (the analysis of that particular sample is not requested). 0001e<ETX> 0000d<ETX> The procedure above explained will be repeated for the first to the 18 th sample tubes before starting the analysis. 3. After measuring (20 seconds about), ROLLER 20 will compile R messages replacing the result field value (0001) with an ESR value between values of 0002 and 0120 or a negative one. For reading errors, the instrument will report the error codes like: -001 for SM error, -002 for NR error and -004 for NF error. <STX>R <STX>R F <ETX> \ <ETX> 4. Host computer should send an ACK confirmation byte ( symbol on hyper terminal program) to ROLLER 20 for having accepted R message. The instrument will ignore it, if ACK option is 0. It will wait for all the set time (TIMEOUT from 2 to 99 seconds) if ACK option is 1. ACK Hex 06 Warnings: 1. If the patient identity (ID) read by the Scanner doesn t find its own correspondent in the Host computer working list, T message will be replaced by <NAK> message Hexadecimal code 21 ( symbol on hyper terminal program), the wheel will however rotate to the next position to allow the operator to insert the tube. The sample will be analysed and R message sent to host. 2. If a particular sample has a very low haematocrit (less then 20%) and * option in the TECH MENU is enabled, the result field of R message sent to the host will be composed by an asterisk in the first position on the left and the rest its own ESR result (i.e. *015 ). If the analysis of a particular sample generates an error, ESR result field will have 0 in the first left position - (minus) for the second position and the rest of the field the error code (i.e ). 3. If no label has been stuck on the tube or the external Scanner cannot read the patient ID, Q message won t be created. The wheel will rotate to accept the sample by pressing ENTER key 17

21 The sample will be however processed and R message sent to host with an identity code generated by itself. The new patient ID in this case will be composed by 10 characters: cycle number (2 characters from 01 to 99), instrument s/n (4 characters), wheel number ( 2 characters) sample position in the wheel (from 01 to 18-2 characters). Example: Instrument s/n Wheel number Cycle number Sample position in the wheel A complete R string for the transmission will be: [Workstation number] [Instrument s/n] [Wheel number] [ESR] [Checksum] <STX>R I <ETX> [Cycle number] [Sample position] [Cycle number (Note 3)] Note 1: If Query Setup option is 0, no Query protocol will be created; the instrument is going to accept and analyse all samples. Note 2: Follow the instruction looking at the Chapter 13-8 of the technical manual for TIMEOUT UART activation. Note 3: This field is related to Bayer parameter on SETUP. If Bayer is 0, the field contains the number of Cycle. If Bayer is 1, the field contains '01' (hex 30 and 31) to keep compatibility with Bayer protocol. Note 4: Checksum value is the result of Exclusive OR (XOR) of all characters of the message, excluded STX, ETX and Checksum. If the Checksum value calculated by the analyzer is 03 (corresponding to ETX), or 02 (corresponding to STX), or 00 (corresponding to NULL), the value will be automatically replaced with 7F hex value. Software House must adopt the same procedure when calculating checksum of the strings received from the analyzer. 18

22 17. ROLLER 10-10PN - 20PN RS232 PORTS features: PORT2 connection. The instrument communicates through Serial Port 1.This port uses a 9 pin male (DB9) serial connector. The used pins are: n 2 for TXDATA, n 3 for RXDATA and 7 for ground. The remaining pins are connected together as nullmodem configuration. This cable must have a D-type 9 pin female connector from one side and a D-type 9 pin female connector at the opposite side. PORT1 connection Serial Port 1 has been reserved for the connection of an external SCANNER The maximum number of ASCII characters for each patient ID is 15 (fifteen). 18. SERIAL CABLE PIN CONNECTIONS TO CONNECT ROLLER10PN/20PN TO PC-LAB: DB9 F DB9 F 19

23 19. ROLLER10PN/20PN PROTOCOL OPTIONS: The choice of the protocol enables the Query functionality. Patient ID codes, having length of characters of 8 digits at maximum, could be read by an external bar code reader (EBCR) or they could be introduced typing them manually. The tables below specify different configurations: (NO QUERY) DAT8 transmission and its examination: Field name Symbol Hex value Length Description STX 02 1 char Start transmission R 52 1 char R states for result message Workstation Is the number identifying the instrument if more than 2 char one is connected to Host computer Patient ID 8 char Patient identification Wheel no. 2 char Indicate the progressive number of analysis made Position 2 char Indicate the sample position inside the wheel Bayer (Note 2) 2 char See note 2 Result 4 char ESR result Checksum (Note 1) 1 char See note 1 ETX 03 1 char End transmission Total length 24 char Checksum note: See note at the end of this page Note2: Bayer field is not used, in this case show s the progressive analysis cycle (NO QUERY) DAT15 transmission and its examination: Field name Symbol Hex value Length Description STX 02 1 char Start transmission R 52 1 char R states for result message Workstation Is the number identifying the instrument if more than 2 char one is connected to Host computer Patient ID 15 char Patient identification Rack no. 2 char Indicate the progressive number of analysis made Position 2 char Indicate the sample position inside the wheel Bayer (Note 2) 2 char See note 2 Result 4 char ESR result Checksum (Note 1) 1 char See note 1 ETX 03 1 char End transmission Total length 31 char Note1: See note at the end of this page Note2:see previous table (QUERY) BCI transmission and its examination: Field name Symbol Hex value Length Description STX 02 1 char Start transmission R 52 1 char R states for result message Workstation Is the number identifying the instrument if more than 2 char one is connected to Host computer Patient ID 15 char Patient identification Rack no. 2 char Indicate the progressive number of analysis made Position 2 char Indicate the sample position inside the wheel Bayer (Note 2) 2 char See note 2 Result 4 char ESR result Checksum (Note 1) 1 char See note 1 ETX 03 1 char End transmission Total length 31 char Note1: See note at the end of this page Note2:see previous table Checksum value is the result of Exclusive OR (XOR) of all characters of the message, excluded STX, ETX and Checksum. If the Checksum value calculated by the analyzer is 03 (corresponding to ETX), or 02 (corresponding to STX), or 00 (corresponding to NULL), the value will be automatically replaced with 7F hex value. Software House must adopt the same procedure when calculating checksum of the strings received from the analyzer. 20

24 NO transmission: PROTOCOL Query DESCRIPTION R message structure SYMBOLS NOTES NO Host NO No transmission In the Communication menu is possible to choose the appropriate protocol and options: To activate the below option the password 1990 is compulsory. From Main Screen, the path to enable it is Setup, Log In Out, LOGIN A keyboard to type the password is going to be displayed. Type the password:1990 then press the left arrow to confirm or Clear to exit without changing anything, in both cases, the instrument is going to display again the previous image, then press Back. 21

25 20. RS232 test to check the communication of ROLLER10PN/20PN To activate the below option the password 1990 is compulsory. From Main Screen, the path to enable it is Setup, Log In Out, LOGIN A keyboard to type the password is going to be displayed. Type the password:1990 then press the left arrow to confirm or Clear to exit without changing anything, in both cases, the instrument is going to display again the previous image, then press Back. Pressing Comm menu and TEST it is possible to send a communication test to host computer through RS232 serial port. If DAT8 is chosen, the test message having 8 ID digits will be sent to the Host like: <0x2>R01TESTR10A U<0x3> <0x2>R01TESTR10B S<0x3> <0x2>R01TESTR10C U<0x3> <0x2>R01TESTR10D ^<0x3> <0x2>R01TESTR10E V<0x3> <0x2>R01TESTR10F T<0x3> <0x2>R01TESTR10G N<0x3> <0x2>R01TESTR10H N<0x3> <0x2>R01TESTR10I H<0x3> <0x2>R01TESTR10J @<0x3> Selecting DAT15 or BCI, the ID digit number to send to the Host is going to be 15 like: <0x2>R01TESTR10A <0x2>R01TESTR10B <0x2>R01TESTR10C <0x2>R01TESTR10D <0x2>R01TESTR10E <0x2>R01TESTR10F <0x2>R01TESTR10G <0x2>R01TESTR10H <0x2>R01TESTR10I <0x2>R01TESTR10J t<0x3> r<0x3> t<0x3> <0x7f><0x3> w<0x3> u<0x3> o<0x3> o<0x3> i<0x3> a<0x3> If NO HOST is configured, the instrument will not communicate with Host Computer 22

26 21. QUERY PROTOCOL project for ROLLER 10-10PN / ROLLER20PN. This paragraph describes the serial protocol (Query Protocol) used by ROLLER10PN /ROLLER20PN with an External Bar Code Reader (EBCR) connected to Serial Port 1 (25 PIN). This protocol is the same used by QUERY TEST1 program; a Windows software designed to be loaded in a Personal Computer (LAB PC) installed in a laboratory and connected to HOST through a network. QUERY protocol uses two files to exchange data for the communication with HOST by using LAB PC like a bridge between ROLLER10PN /ROLLER20PN and HOST Computer. Further information of QUERY TEST1 program software can be taken from the QUERY TEST1 installation and using manual. Fields to check looking at the flag list. 23

27 22. Protocol explanation (BCI Configuration): 1. The patient bar code ID stuck on each sample tube can be read by using an External Bar Code Reader connected to Roller 10 Serial Port1. The instrument will create and send Q <STX>Q a<ETX> message to HOST computer through RS232 serial port (or to LAB PC if it is used like a bridge) if Query Setup option is 1. (Note 3) 2. HOST computer verifies if the patient identity has been inserted in the working list and then it replies with "T" message. Roller 10 remains waiting for receiving it on time (within 2 seconds), if ACK option is disabled. It will wait for all the set time if ACK option is enabled. (Follow the instruction looking on page 22 of this manual.) 3. As soon as the instrument receives T message, it is checked. 4. The wheel is going to rotate to one position to allow the operator to insert the sample tube, if T message has the result field compiled with 0001 (that means the sample requires the <STX>T <STX>T e<ETX> 0000d<ETX> analysis). The wheel will remain stop, if the result field is 0000 (the analysis of that particular sample is not requested). Before starting the analysis, the explained above, from 1 to 2, procedure will be repeated for the further 10 th (Roller 10) or the 20 th (Roller 20) sample tubes. 5. After measuring (20 seconds about), Roller 10 compile R messages replacing the result field value (0001) with an ESR value that, read on the paper could be between values of 0002 and 0120 or a negative one. For reading errors, the instrument will report the error <STX>R <STX>R f <ETX> l <ETX> codes like: for SM error, -002 for NR error and -004 for NF error. 6. Host computer should send an ACK confirmation byte ( symbol looking at the hyper terminal program) to Roller 10 for having accepted R message. The Hexadecimal code 0x06 instrument will ignore it, if ACK option is disabled. It will wait for all the set time (TIMEOUT from 2 to 60 seconds) if ACK option is enabled. Protocol explanation (DAT 8 DAT 15 Configuration): In this case the instrument doesn t query the Host Computer, it just perform the analisis and send the result to host computer as explaned at points 3 and 4. Warnings: 1. If the patient identity (ID) read by the Scanner doesn t find its own correspondent in the Host computer working list, T message will be replaced by <NAK> message ( symbol looking at the hyper terminal program). If MISS ID option is enabled, the wheel Hexadecimal code 0x21 will rotate to the next position to allow the operator to insert the tube. The sample, then will be analysed and R message sent to host. 2. If no label has been stuck on the tube or the external Scanner cannot read the patient ID, Q message won t be created. The wheel is going to rotate to accept the sample tube by pressing Next button on touchscreen display. 3. The sample is going to be processed and R message sent to host with an identity code generated by itself. The new patient ID in this case will be composed by 10 characters: 4. Cycle number (2 characters from 01 to 99), instrument s/n (4 characters), wheel number ( 2 characters) sample position in the wheel (from 01 to 10-2 characters). 24

28 Example: Instrument s/n Wheel number Cycle number Sample position in the wheel A complete R string for the transmission will be: [Workstation number] [Instrument s/n] [Wheel number] [ESR] [Checksum] <STX>R f <ETX> [Cycle number] [Sample position] [Cycle number (Note 5)] Note 1: Note 2: Note 3: Note 4: Note 5: Checksum value is the result of Exclusive OR (XOR) of all characters of the message, excluded STX, ETX and Checksum. If the Checksum value calculated by the analyzer is 03 (corresponding to ETX), or 02 (corresponding to STX), or 00 (corresponding to NULL), the value will be automatically replaced with 7F hex value. Software House must adopt the same procedure when calculating checksum of the strings received from the analyzer. <STX> character ( symbol looking at the hyper terminal program) in the serial transmission is represented by 0x02 hexadecimal bytes and <ETX> ( symbol looking at the hyper terminal program) by 0x03. If Query Setup option is 0, no Query protocol will be created; the instrument will accept and analyse all samples. Follow the instruction looking at the of the technical manual for TIMEOUT UART activation. This field is related to Bayer parameter on SETUP. If Bayer is 0, the field contains the number of Cycle. If Bayer is 1, the field contains '01' (hex 30 and 31) to keep compatibility with Bayer protocol. 25

29 23. TEST1, ROLLER10PN-ROLLER20PN and ROLLER20(old model) Multiple connections Query protocol is able to manage multiple connections by using the same PC-LAB with multiple serial ports. In that case every instrument connected to the PC-LAB has to have an identification number different for each and the PC-LAB the same number of serial ports as the instruments. Our instruments have an option (TEST1 number in the COMMUNICATION menu (for TEST1) and ROLLER number in the TECH MENU (for ROLLER20) to assign a different identification number to each instrument, R message should be different for each like in the following example: [Workstation number] <STX> R [Workstation number] <STX> R [Workstation number] <STX> R [Checksum] I <ETX> [Checksum] z <ETX> [Checksum] ~ <ETX> 26

30 24. INTERFACING LATEX PROTOCOL LATEX PROTOCOL INTERFACE EXPLICATION Up to Sw. version 6.01C (Test 1), 6.01B (Roller20 old model) The three R Latex messages are sent, to the serial port, after having analysed the three referent Latex of the Control kit and at the end of the washing, by withdrawing water from the fifth and sixth inserted tube, for rinsing the capillary. It should be understood R Latex messages are not available by using Calibration Latex kit. Messages R message for Latex Field name Hex Length value Description STX (Note 2) 02 1 char R 52 1 char Means the instrument is sending a Result Workstation 2 char Identifies the workstation if more instrument are connected, normally is 01 LATEXSTDL 9 char Is the identification of the latex tube Field 1 1 char Can be 1, 2 or 3 and identifies level 1, 2 and 3 of latex ESR Value 3 char This is the expected Latex ESR value Space char Two spaces Field 2 2 char Fixed to 01 Field 3 2 char The same as Field 1 Field 4 2 char The same as Field 2 ESR Value 4 char This is the detected Latex ESR value Checksum (Note 1) 1 char ETX 03 1 char Total length 31 char Note 1: Note 2: Checksum value is the result of Exclusive OR (XOR) of all characters of the message, excluded STX, ETX and Checksum. If the Checksum value calculated by the analyzer is 03 (corresponding to ETX), or 02 (corresponding to STX), or 00 (corresponding to NULL), the value will be automatically replaced with 7F hex value. Software House must adopt the same procedure when calculating checksum of the strings received from the analyzer. <STX> character ( symbol looking at the hyper terminal program) in the serial transmission is represented by 0x02 hexadecimal bytes and <ETX> ( symbol looking at the hyper terminal program) by 0x03. here are some examples: <0x2>R01LATEXSTDL ^<0x3> <0x2>R01LATEXSTDL ><0x3> <0x2>R01LATEXSTDL ><0x3> 27