# Introduction to the workbook and spreadsheet

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3 unique cell in the spread sheet, the contents of the cell can then be used elsewhere in the program simply by referencing the cell address. Entering data or labels into cells is simple, just move the cursor to the cell you wish to enter your datum, click to select the cell, enter your datum, and press enter. It's important to note that you must press 'enter'; otherwise the spread sheet does not recognize that you have entered data. If you wish to enter a series of numbers you can speed up the process by using the auto fill capability. To use auto fill, enter the first two numbers in the series in adjoining cells. Now select both cells, grab the common handle (the little black box in the bottom right hand corner of the selected cells) and drag down as far as needed. You should now have a series of numbers, following the pattern of the first two you entered. This trick will work for letters and formulas as well as numbers, and works for columns as well as rows. Handle Before auto fill After auto fill Figure 2. Using Excel's auto fill. For example; if you sampled every 30 sec, enter 30 sec in A1 and 60 sec in A2. Select both cells, grab the handle and drag down to cell A8. You should now have a series of numbers, each 30 more than the one above it, as picture in the right hand screen shot. Making your spread sheet look pretty. To resize columns and rows, click in the header cell to select the column or row and drag the margin. Or, right click the header cell and select the column width, row height option. Hundreds of options exist for formatting cells. Most of these are accessed by selecting the cells you wish to format, right clicking, and selecting the format cells option. Especially check out the options in the Number and Alignment tabs. Page 3 of 13

5 Table 3. Operator precedence in Excel. Precedence Operator Description 1 : (colon) (single space), (comma) Reference operators 2 Negation (as in 1) 3 % Percent 4 ^ Exponentiation 5 * and / Multiplication and division 6 + and Addition and subtraction 7 & Connects two strings of text (concatenation) 8 = < > <= >= <> Comparison Using Excel's functions The easiest way to understand the implementation of Excel functions is by following a step by step example. To access Excel's functions, click the down arrow next to the sum button. As shown in Figure 3, this gives you a popup menu showing the five most common Excel functions, and below these, a menu choice titled 'More Functions". Note that selecting one of the five functions in the pop up menu will work differently then selecting them from the "More Functions" menu. In this first example we will calculate the sum of a series of numbers. Figure 3. Excel's pop up function menu. Step 1. Start by entering the series of numbers as pictured in Figure 3. Place your cursor in cell A7. Select sum from the list of functions that appears when you click the down arrow next to the sum button (or click the sum button). Excel tries to guess the cells you wish to sum up. Generally it will select all the cells containing numerical data immediately next to the cell you are inserting the function into. Page 5 of 13

6 Figure 4. Excel's sum function. Step 2. You can see what cells Excel has chosen in 2 ways. They will be enclosed in a marching dash box, and the range is displayed in the function window. In this case Excel has chosen the correct data. You can always override by selecting the cells yourself, or typing the correct range in the function window. Step 3. When the correct cells have been chosen, press enter. The sum will appear in cell A7. Note that when you select cell A7, the function appears in the function window, but the result will still appear in the cell on the spread sheet Figure 5. Result of using Excel's sum function. Page 6 of 13

7 In this second example, we will calculate the standard deviation for the same numbers used in the sum example. Figure 6. Excel's insert function window. Step 1. Place your cursor in cell A8. From the function pop up menu choose more functions. Step 2. When you select 'More Functions', you will get a new window where you can either search for the function by name, or select a category, and then scroll down looking for the function. Notice the description of the selected function appears near the bottom of the window. Enter 'standard deviation' in the search window and click 'Go'. Select the function STDEV. Figure 7. Excel's Function Arguments window. Step 3. Once you have selected the function you want, you will get yet a new window called 'Function Arguments '. Here the program is looking for the address, or actual numbers, you want the function to use. You can either enter the cells addresses manually, or select the cells, using click and drag. Go back to the spread sheet and select the cells A1 through A6. Your Function Arguments window should now look like the one in Figure 7. Select OK. Cell A8 will now show the standard deviation (4.1833) for cells A1 through A6. Using auto fill to copy a formula. Often, you will want to apply the same formula to a series of cell. Enter the weight data as shown in Figure 8 (don't enter the mean weight). Use Excel's average function to calculate the average for sample 1. Now, grab the cell handle for Sample 1 mean weight and drag down four cells. Go back and click in the cell for the mean weight of sample 2. Notice that the formula bar, the function is still AVERAGE, but the cell reference has moved down one row (in my example it would now read B5:E5). This is referred to as relative addressing and it is the default method Excel applies to copying formulas. Page 7 of 13

8 Figure 8. Auto filling a formula in Excel. Occasionally you will need to make an absolute reference to a cell. To do this, add dollar signs to the cell reference. In my example I need to correct the mean weight of all the samples by multiplying it by the Z factor of , which is entered in cell 1I. To do this, I enter a formula, as shown in the formula bar of Figure 9, using \$I\$1 to reference the Z factor. Now, when I auto fill, all mean values are multiplied by the value Give it a try, and see what happens when you don't use the absolute reference. Figure 9. Using an absolute reference in Excel. Box 2. Entering formulas. All formulas start with an = sign. Case is not important when entering the formula. Cells containing non numerical entrees will be ignored in calculations. Excel functions are listed in; Excel Help>Contents>Function Reference The default for auto filling formulas is to use relative addressing. Page 8 of 13

9 Graphing Excel has the capability of making many different styles of graphs. The following example will show you how to make a scatter plot, add a linear regression trend line, and how to fine tune the graphs appearance. Making a scatter plot. Graph Wizard Step 1. Let's start with a typical set of data for establishing a standard curve. Enter the data into Excel as picture in Figure 10. Now click the chart wizard button. Click the XY scatter plot button. Do not use line graph, this will not give us what we want. Select the first option for graph sub-type (the one with just dots, no line drawn), and click next. Figure 10. Excel's chart wizard, step 1, selecting the chart type. Step 2. Excel will guess what data you wish to use. It may or may not guess correctly. To check what data has been selected, click the series tab. The series window is also where you would go to add more data. Don't worry about the series name for now. Figure 11. Excel's Chart Wizard, step 2, selecting the source data. Page 9 of 13

10 Click the small box containing the red arrow next to the X value window. This button takes you back to the spreadsheet. The data being used as X values will be in a marching dash box. If you need to change the data being used, just select it. Similarly, check the Y data. Remember, your X axis is always the controlled variable (in this case the protein concentration). Figure 12. Excel's Chart Wizard, step 2, selecting the source data. This button appears in many Excel source data windows, clicking it will always take you back to the spread sheet, allowing you to select cells for input. Clicking this button will take you back to source data window you started from. Figure 13. Excel's Chart Wizard, step 3. Step 3. When you are satisfied with the data being used, click next. This takes you to the Chart Options window. Notice the multiple tabs for formatting your graph. Enter titles for the X and Y axis, and a chart title. In the Gridlines tab, remove the major gridlines. Since there is only one series, go to the legend tab and remove the legend. Click next. Step4. Choose where to place your chart in the work book. It's usually best to use the default, object in sheet, as the graph appears next to your data. Page 10 of 13

11 Standard curve for protein concentration. OD [protein] mg/ml Figure 14. Excel graph before fine tuning. Page 11 of 13

13 Adding error bars to a chart. Select the data series to which you want to add error bars. To do this, click on of the points on the graph belonging to the data series. On the Format menu, click Selected Data Series. On the X Error Bars tab or the Y Error Bars tab, select the options you want. Box 3. Graphing basics. Once your graph is made it will automatically be updated to reflect any changes you make to the data used to create the graph. To modify most things on your graph either right click the chart, or double click the element you wish to change. To access chart specific menus, the chart must be selected. Page 13 of 13

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