Urine Preservatives Collection and Transportation for 24-Hour Urine Specimens

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1 Preservatives Collection and Transportation for 24-Hour Specimens The Department of Laboratory Medicine and Pathology at Mayo Clinic initiated a study to determine stability of analytes under different preservative conditions. This reference chart, built from that study, will enable you to select either temperature conditions or preservatives to maximize the number of tests that can be performed on a single 24-hour collection. Note: See the Test Catalog for test-specific collection requirements. Refrigerate Frozen Aldosterone, 24 ALDU OK OK OK OK Preferred OK No No No OK No Aldosterone with Sodium, 24 Alpha-1-Microglobulin, 24 ALDNA OK OK OK OK Preferred OK No No No OK No A1M24 Preferred OK OK OK OK OK Yes No No OK Preferred Aluminum, 24 ALU OK Preferred OK No OK OK No OK OK No OK Arsenic, 24 ASU OK Preferred OK No OK OK No OK No No OK Arsenic Fractionation, 24 Arylsulfatase A, 24 Hour, 11 Beta-Prostaglandin F2 Alpha, 24 ASFR OK Preferred OK No OK OK No OK No No OK ARSU No Required No No No No No No No No No BPG2 No OK OK OK Preferred OK No OK OK OK No Cadmium, 24 CDU OK Preferred OK No OK OK No OK OK No OK Calcium, 24 CAU OK Preferred OK OK OK OK No OK OK No OK Calcium/Creatinine, 24 Hour, Catecholamine Fractionation, Free, 24 CACU OK Preferred OK OK OK OK No OK OK No OK CATU No OK OK OK Preferred No No OK OK OK No Chloride, 24 CLU OK Preferred OK OK OK OK No No No OK Preferred 28 days 28 days Frozen NO Frozen Preferred 14 days Refrig OK 28 days 14 days 14 days Page 1 of 6 06/2015 Refrig OK

2 Preservatives Collection and Transportation for 24-Hour Specimens Refrigerate Frozen Chromium, 24 CRU OK Preferred OK No No No No No No No OK Citrate Excretion, 24 Hour, CITR No OK OK OK No Preferred No No No OK No Cobalt, 24 COU OK Preferred OK No OK OK No OK OK No OK Copper, 24 CUU OK Preferred OK No OK OK No OK OK No No Cortisol/Cortisone, Free, 24 COCOU No OK OK Preferred OK OK OK No No OK No Cortisol, Free, 24 CORTU No OK OK Preferred OK OK OK No No OK No Creatinine, 24 Creatinine Clearance, Serum and 24-Hour Cystinuria Profile, Quantitative, 24 Electrophoresis, Protein, 24 Electrophoresis, Protein, 24 INTERNAL ONLY CTU Preferred OK OK OK OK OK OK OK OK OK Preferred CRC Preferred OK OK OK OK OK OK OK OK OK Preferred CYSQN No OK OK No No Preferred No No No No No EPU OK Preferred OK No No OK No No No OK Preferred PEU OK Preferred OK No No OK No No No No Preferred Gadolinium, 24 GDU OK Preferred OK No No OK No OK OK No OK Glucose, 24 GLUR No OK OK Preferred OK OK No OK OK OK No Heavy Metals Screen, 24 Homovanillic (HVA), 24 5-Hydroxyindoleacetic (5- HIAA), days 28 days 28 days Refrig Preferred 30 days 30 days 30 days Refrig Preferred - Frozen Preferred 14 days Refrig with Toluene OK 3 days 3 days 28 days HMSU No Preferred OK No OK OK No OK No No No HVA No No No No Preferred No No OK No No No HIAA No OK OK OK Preferred OK OK OK OK OK No Refrig Preferred 56 days 365 days Page 2 of 6 06/2015

3 Preservatives Collection and Transportation for 24-Hour Specimens Immunofixation, 24 Hour, Immunoglobulin Total Light Chains, Inherited Conjugated Hyperbilirubinemias, 24 Hour, Refrigerate Frozen IFXU OK Preferred OK No No OK No No No OK Preferred TLCU OK <72 Hours Preferred OK No No No No No No No <72 Hours OK CI No No No No No No Required No No No No Iodine, 24 UIOD OK Preferred OK No OK No No No No No OK Iron, 24 FEU No Preferred OK No OK OK No OK OK No No Lead, 24 PBU OK Preferred OK No OK OK No OK OK No OK Magnesium, 24 MGU OK Preferred OK OK OK OK No OK OK No OK Manganese, 24 MNU OK Preferred OK No No No No No No No OK Mercury, 24 HGU No Preferred OK No OK OK No OK OK No No Metanephrines, Fractionated, 24 N-Methylhistamine, 24 Hour, Microalbuminuria, 24 Hour, METAF No OK OK Preferred Preferred OK OK OK OK OK OK NMHIN OK Preferred OK OK OK OK OK OK OK OK OK MA24 Preferred OK OK OK No OK No No No OK OK Molybdenum, 24 MOLU OK Preferred OK No OK OK No OK No No OK Monoclonal Protein Study, 24 MPSU OK Preferred OK No No OK No No No OK Preferred Nickel, 24 NIU OK Preferred OK No OK No No No OK No OK Nitrogen, Total, 24 Hour, NTX-Telopeptide, 24 Hour, NITU OK OK Preferred No OK OK OK OK No OK Preferred NTXPR No OK <5 days Preferred OK No OK No No No No No 3 days 20 days 3 days Frozen Preferred Refrig NO Refrig Preferred 146 days 146 days 146 days 28 days Refrig Preferred 8 days 2 days 28 days 3 days 28 days Frozen Preferred 30 days Refrig OK 5 days Page 3 of 6 06/2015

4 Preservatives Collection and Transportation for 24-Hour Specimens Refrigerate Frozen Oxalate, 24 OXU No OK OK No No Preferred No No No OK No Phosphorus, 24 POU OK Preferred OK OK OK OK No OK OK OK Preferred ph, 24 UPH24 No OK OK No No Preferred No No No OK No Porphyrins, Quantitative, 24 PQNU No No No No No No Required No No No No Potassium, 24 KUR OK Preferred OK OK OK OK OK OK OK OK Preferred Protein, Total, 24 PTU OK Preferred OK OK No OK No No No OK Preferred Retinol-Binding Protein, 24 RBP24 Preferred OK OK OK No OK No No No OK Preferred Selenium, 24 SEU OK Preferred OK No OK OK No OK OK No OK Serotonin, 24 SERU No Preferred OK No Preferred OK No No OK OK OK Sodium, 24 NAU OK Preferred OK OK OK OK No OK OK OK Preferred Sulfate, 24 SULFU No Preferred OK OK No OK OK No No OK Preferred Supersaturation Profile, 24 SSAT No OK OK No No Preferred No No No No No Thallium, 24 TLU OK Preferred OK No OK OK No OK OK No OK Titanium, 24 TIU OK Preferred OK No OK No No OK OK No OK Urea, 24 URAU No OK OK Preferred OK OK OK OK OK OK No Uric, 24 URCU No OK OK Preferred No OK OK No No OK No Protect from Light Frozen NO Frozen Preferred 28 days Refrig OK 28 days 28 days Frozen Preferred 14 days Refrig OK 28 days 28 days Refrig Preferred Refrig OK Protect from Light Page 4 of 6 06/2015

5 Preservatives Collection and Transportation for 24-Hour Specimens Refrigerate Frozen Vanadium, 24 VU OK Preferred OK No No No No No No No No Vanillylmandelic (VMA), 24 Xanthine and Hypoxanthine, 24 VMA No No No OK Preferred No No OK OK No No XAN No OK Preferred No No No No No No No No Zinc, 24 ZNU OK Preferred OK No OK OK No OK OK No OK Frozen Preferred Refrig OK 28 days Page 5 of 6 06/2015

6 Preservatives Collection and Transportation for 24-Hour Specimens The conditions and concentrations of urine preservatives are defined for 24-hour collections as follows: Room temperature (21-25 degrees C) Refrigerated 4 degrees C Frozen -20 degrees C 6N HCl 30 ml per 24-hour collection 25 ml per 24-hour collection Na 2 CO 3 (crystals) 5 g per 24-hour collection Toluene 30 ml per 24-hour collection 6N HNO 3 15 ml per 24-hour collection (crystals) 10 g per 24-hour collection Thymol (10% in isopropanol) 10 ml per 24-hour collection Preservative and condition guidelines have been evaluated specifically for Mayo Medical Laboratories assays. If you have questions regarding the use of preservatives, contact Mayo Medical Laboratories at or Page 6 of 6 06/2015