Urine Preservatives Collection and Transportation for 24-Hour Urine Specimens

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1 The Department of Laboratory Medicine and Pathology at Mayo Clinic initiated a study to determine stability of analytes under different preservative conditions. This reference chart, built from that study, will enable you to select either temperature conditions or preservatives to maximize the number of tests that can be performed on a single 24-hour collection. Note: See the Test Catalog for test-specific collection requirements. *Protect from light. Aldosterone, 24 ALDU OK OK OK OK Preferred OK No No No No No Aldosterone with Sodium, 24 ALDNA OK OK OK OK Preferred OK No No No No No Alpha-1-Microglobulin, 24 A1M24 OK Preferred OK OK OK OK No No No OK OK Aluminum, 24 ALU OK Preferred OK No OK OK No OK OK No OK Ammonium, 24 AMMO No OK OK No No Preferred No No No No No Arsenic, 24 ASU OK Preferred OK No OK OK No OK No No OK Arsenic Fractionation, 24 ASFR OK Preferred OK No OK OK No OK No No OK Arylsulfatase A, 24 Hour, ARSU No Required No No No No No No No No No Cadmium, 24 CDU OK Preferred OK No OK OK No OK OK No OK Calcium, 24 CALU OK Preferred OK OK OK OK No OK OK OK OK Catecholamine Fractionation, Free, 24 CATU No OK OK OK Preferred No No OK OK No No Chloride, 24 CLU OK Preferred OK OK OK OK No No No OK OK Chromium, 24 CRU OK Preferred OK No No No No No No No OK 14 days 28 days 72 hours 28 days Frozen NO 28 days 72 hours Page 1 of 5 12/ days

2 Citrate Excretion, 24 Hour, CITR No OK OK OK No Preferred No No No OK No Cobalt, 24 COU OK Preferred OK No OK OK No OK OK No OK Copper, 24 CUU OK Preferred OK No OK OK No OK OK No No Cortisol/Cortisone, Free, 24 COCOU No OK OK Preferred OK OK No No No No No Cortisol, Free, 24 CORTU No OK OK Preferred OK OK No No No No No Creatinine, 24 CTU OK Preferred OK OK OK OK OK OK OK OK Preferred Creatinine Clearance, Serum and 24-Hour CRC Preferred OK OK OK OK OK OK OK OK OK Preferred Cystinuria Profile, Quantitative, 24 CYSQN No OK OK No No Preferred No No No No No 28 days 28 days 72 hours Refrig Preferred 30 days 30 days Refrig Preferred Frozen Preferred 14 days Refrig with Toluene OK 2,3-Dinor-11Beta- Prostaglandin F2 Alpha, 23BPG No Preferred OK OK OK OK OK No No No No 8 hours Electrophoresis, Protein, 24 EPU OK Preferred OK No No OK No No No OK OK Electrophoresis, Protein, 24 INTERNAL ONLY PEU OK Preferred OK No No OK No No No OK OK Gadolinium, 24 GDU OK Preferred OK No No OK No OK OK No OK Glucose, 24 GLUR No OK OK Preferred OK OK No OK OK OK No Heavy Metals Screen, 24 HMSU No Preferred OK No OK OK No OK No No No Homovanillic (HVA), 24 HVA No No No No Preferred No No OK No No No 5-Hydroxyindoleacetic (5- HIAA), 24 HIAA No OK OK OK Preferred OK OK OK OK OK No 72 hours 72 hours 28 days 180 days Refrig Preferred 56 days 365 days Refrig OK Page 2 of 5 12/2017

3 Immunofixation, 24 Hour, IFXU OK Preferred OK No No OK No No No OK OK 72 hours Immunoglobulin Total Light TLCU OK <72 Hours Preferred OK No No No No No No No <72 Hours OK 20 days Chains, 72 hours Iodine, 24 UIOD OK Preferred OK No OK No No No No No OK Lead, 24 PBU OK Preferred OK No OK OK No OK OK No OK Leukotriene E4, LTE4 No Preferred OK OK OK OK OK No No No OK Magnesium, 24 MAGU OK Preferred OK OK OK OK No OK OK OK OK Manganese, 24 MNU OK Preferred OK No No No No No No No OK Mercury, 24 HGU No Preferred OK No OK OK No OK OK No No Metanephrines, Fractionated, 24 METAF No OK OK Preferred Preferred OK OK OK OK No No N-Methylhistamine, 24 Hour, NMHIN OK Preferred OK OK OK OK OK OK OK No OK Microalbuminuria, 24 Hour, MA24 OK Preferred OK OK No OK No No No OK OK Monoclonal Protein Study, 24 MPSU OK Preferred OK No No OK No No No OK OK Nickel, 24 NIU OK Preferred OK No OK No No No OK No OK Nitrogen, Total, 24 NITU OK OK Preferred No OK OK OK OK No OK OK Oxalate, 24 OXU No OK OK No No Preferred No No No OK No Phosphorus, 24 POU OK Preferred OK OK OK OK No OK OK OK Preferred Refrig Preferred 146 days 146 days 146 days 28 days 24 hours 72 hours Refrig Preferred 8 days 24 hours 72 hours 28 days Page 3 of 5 12/2017

4 ph, 24 UPH24 No OK OK No No Preferred No No No OK No Porphyrins, Quantitative, 24 PQNU No No No No No No Required* No No No No Potassium, 24 KUR OK Preferred OK OK OK OK OK OK OK OK Preferred Protein, Total, 24 PTU No Preferred OK OK No OK No No No OK Preferred Retinol-Binding Protein, 24 RBP24 Preferred OK OK OK No OK No No No OK Preferred Serotonin, 24 SERU No Preferred OK No Preferred OK No No OK No OK Sodium, 24 NAU OK Preferred OK OK OK OK No OK OK OK Preferred Sulfate, 24 SULFU No Preferred OK OK No OK OK No No OK OK Supersaturation Profile, 24 SAT24 No OK OK No No Preferred No No No No No Thallium, 24 TLU OK Preferred OK No OK OK No OK OK No OK Urea, 24 URAU No OK OK Preferred OK OK OK OK OK OK No Uric, 24 URCU No OK OK Preferred No OK OK No No OK No Vanillylmandelic (VMA), 24 VMA No No No OK Preferred No No OK OK No No Zinc, 24 ZNU OK Preferred OK No OK OK No OK OK No OK Frozen Refrig NO Frozen Preferred 14 days Refrig OK 28 days 180 days 28 days Refrig OK Protect from light Page 4 of 5 12/2017

5 The conditions and concentrations of urine preservatives are defined for 24-hour collections as follows: Ambient Room temperature (21-25 degrees C) Refrigerated 4 degrees C Frozen -20 degrees C 6N HCl 30 ml per 24-hour collection 50% Acetic 25 ml per 24-hour collection Na 2 CO 3 (crystals) 5 g per 24-hour collection Toluene 30 ml per 24-hour collection 6N HNO 3 15 ml per 24-hour collection Boric (crystals) 10 g per 24-hour collection Thymol (10% in isopropanol) 10 ml per 24-hour collection Preservative and condition guidelines have been evaluated specifically for Mayo Medical Laboratories assays. If you have questions regarding the use of preservatives, contact Mayo Medical Laboratories at or Page 5 of 5 12/2017