Scientific Cameras. scmos and Scientific CCD Cameras. Product Family. Scientific imaging requires highperformance
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1 Scientific Cameras scmos and Scientific CCD Cameras Scientific imaging requires highperformance cameras. Thorlabs Scientific-Grade Cameras are specifically designed for microscopy and other demanding applications. Based on high quantum efficiency, lownoise imagers, our cameras are ideal for multispectral imaging, fluorescence microscopy, and other imaging techniques. Cooled CCD Non-Cooled CCD Quantalux scmos Product Family u Cameras Available Quantalux TM scmos with <1 e - Read Noise Scientific CCDs: Fast Frame Rate VGA, 1.4 MP, 4 MP, and 8 MP u Monochrome or Color Sensor u Fanless Passive and TE-Cooled Options u USB 3., Gigabit Ethernet, or Camera Link Interface u Simple Mechanical and System Integration u ThorCam TM Software, API/SDK, and Third- Party Support
2 Solutions for Your Imaging Needs Our Cameras Feature Industry- Standard C-Mount Threading for Direct Compatibility with Most Microscopes Feature Optical & Imaging A Choice of Five High Quantum Efficiency, Low-Noise Sensors: u 2.1 Megapixel scmos (Monochrome; Color Available Late 17) CCD (Monochrome or Color) u 4 Megapixel CCD (Monochrome or Color) u 8 Megapixel CCD (Monochrome or Color) u fps Fast Frame Rate, VGA Resolution CCD (Monochrome) Removable Infrared Filter or Window Included High Quantum Efficiency (See Specifications for Details) Low Read Noise (See Specifications for Details) System Integration Software-Selectable Pixel Clock Speed Asynchronous Reset and Triggered Modes Bulb Exposure Mode Region of Interest (ROI) Mode Binning Mode Thermal Management: u Hermetically Sealed Chamber with Fanless TE-Cooling (Cooled Version of CCD Cameras) u Fan and TEC-Free Passive Thermal Management (scmos Cameras) C-Mount (1."-32) Threaded Lens Mount USB 3., Gigabit Ethernet, or Camera Link interface Robust Design with Small Form Factor Compatible with Thorlabs Cage System Auxiliary Port and Available Cables and Accessories Software ThorCam TM Software GUI Third-Party Software Support Including LabVIEW, µmanager/ ImageJ, Metamorph, and MATLAB Supported by Full-Featured API/SDK Benefit Choose the Camera with the Resolution and Frame Rate Ideal for Your Application (Turn the Page to See Application Examples) Remove the Filter for NIR Applications, or Replace with Any Ø25 mm Filter to Image Wavelengths of Interest Maximizes Camera Output and Improves SNR for a Given Amount of Light at Wavelengths of Interest Improves the Threshold of Detectability Under Low Light Conditions Maximize Frame Rate for Fast Imaging or Select Slower Readout to Minimize Noise Complete Timing Control for Flexible System Integration Control the Duration and Instant of Exposure with a Single Input Pulse Select a Sub-Frame Rectangular Region for Faster Readout without Sacrificing Spatial Resolution Allows a Lower-Noise, Faster Readout of the Entire Frame at a Lower Spatial Resolution Ideal for Low Signal Levels and/or Long Exposures; No Additional Vibration During Long Exposures Integrate Cameras with Microscopes or Lenses with C-Mount Threads Industry-Standard, Robust, High-Bandwidth Interfaces Easily Integrates with Existing Equipment Integrate Cameras into a Custom Imaging System Eases System Integration and Timing for Unique Situations One Package for System Control, Acquisition, and Playback of Images and Image Sequences Integrate Thorlabs Cameras into a Third-Party Imaging Platform of Choice Ideal for System Integrators and Developers of Custom Applications
3 Camera Overview Quantalux scmos Cameras Thorlabs 2.1 Megapixel Quantalux scmos cameras are based on highperformance, 1 e - read noise imagers. Ideal for demanding applications, the monochrome sensor (color available late 17) can image the full 19 x 8 frame at 5 fps with 16 bit resolution and offer a peak quantum efficiency of 61% at 6 nm. Packaged in a housing that measures 2.38" x 2.78", each scmos camera is equipped with passive thermal management, reducing dark current without the need for a cooling fan or thermoelectric cooler. A USB 3. interface provides compatibility with most computers. The compact housing is feature-rich, enabling seamless integration into a multitude of setups. An adjustable C-Mount adapter is factory installed into the SM1-threaded optical aperture of the camera for out-of-the-box compatibility with industry-standard microscopes and camera lenses. Various mounting taps are also provided for optical post and 3 mm cage system compatibility. Scientific-Grade CCD Cameras Thorlabs scientific CCD cameras are offered in two package styles: a noncooled package and a hermetically sealed package with a two-stage TEC. The fan-free cooler design provides optimal CCD cooling without vibration, critical for capturing long-exposure images in low-light conditions. Each CCD camera is available with either a USB 3., Gigabit Ethernet (GigE), or Camera Link interface. GigE is ideal when the camera must be far from the PC or when there are multiple cameras that need to be controlled by the same PC. The USB 3. and Camera Link interfaces offer higher maximum data transfer rates. CCD cameras feature standard C-Mount threading, and Thorlabs provides a full line of thread-to-thread adapters for compatibility with other thread standards. The front face is also equipped with 4- tapped holes for compatibility with our 6 mm cage system. Four 1/4"- tapped holes, one on each side of the housing, are compatible with our Ø1" Posts. These features make Thorlabs cameras an ideal choice of CCD imager for both DIY and commercial imaging systems for microscopy. Quantalux scmos Camera Hermetically Sealed, Cooled CCD Camera Non-Cooled CCD Camera Our Quantalux scmos Cameras can be integrated into our 3 mm cage system to construct custom imaging systems. Scientific CCD cameras can be integrated into our 6 mm cage system. A C-Mount lens mount allows integration with our family of machine vision camera lenses. Thorlabs thread adapters and lens tubes can be used to create unique solutions. Here, a scientific CCD camera is integrated with our our SM1 lens tubes and Cerna modular microscopy platform for custom epi-fluorescence imaging.
4 Images from Selected Applications Intracellular Dynamics Fast Frame Rate Cameras can be used for Ca 2+ ratiometric studies of intracellular dynamics. Highspeed imaging is made possible by the fast frame rate of the camera as two excitation wavelengths are switched in rapid succession. Alternately, quantitative imaging data can be acquired from fluorescence emission at two distinct wavelengths. Brightfield Microscopy A brightfield microscopy image showing Ki-67 labeled tonsil cells. Ki-67 is an antigen that only appears in the nuclei of cells undergoing division; therefore, it is an excellent marker to indicate the growth fraction of a cell population. u Quantalux TM scmos u Fast Frame Rate, VGA u High QE u Low Noise u ROI and Binning Modes u 4 Megapixel u 8 Megapixel u High Resolution u Large Field of View Ophthalmology (NIR) Retinal/fundus imaging in the NIR to view the blood vessels in the eye. u Quantalux TM scmos u NIR Responsivity u Low Noise Neuroscience Fluorescence image of a rat neuron using X magnification. u Quantalux TM scmos u High QE u Low Noise u Thermal Management
5 u Quantalux TM scmos u 4 Megapixel u High QE u Low Noise u Thermal Management u Quantalux TM scmos u 4 Megapixel u High QE u Low Noise Flurorescence Microscopy Merged triple emission fluorescence microscopy image. The sample slide consists of multi-labeled bovine pulmonary artery endothelial cells (BPAEC), showing at least one example of a double nucleus. Multispectral Imaging A series of multispectral images taken with different passband wavelengths; the final stacked color image is shown. High QE scientific cameras are especially beneficial for obtaining low light, narrowband images. This image was acquired using a Thorlabs KURIOS-WB1 Liquid Crystal Tunable Filter. u 4 Megapixel u Fast Frame Rate, VGA u High QE u ROI and Binning Modes Scanning Electron Microscopy Scanning Electron Microscope (SEM) image of a nickel sample. Electron Backscatter Diffraction (EBSD) results in Kikuchi patterns that result from the interaction between the electron beam and the sample material. Our high QE, low noise cameras make possible high speed detection and analysis of these faint line patterns against relatively high backgrounds. u Quantalux TM scmos u NIR Responsivity u High QE u Low Noise Simultaneous NIR/DIC and Fluorescence The image shows a live, simultaneous overlay of fluorescence and NIR Dodt contrast images of a 5 µm brain section from a CX3CR1-GFP mouse, which has been immunostained for PECAM-1 with Alexa-687 to highlight vasculature. The Dodt contrast uses a quarter annulus and a diffuser to create a gradient of light across the sample that can reveal the structures of thick samples. Sample courtesy of Dr. Andrew Chojnacki, Department of Physiology and Pharmacology, Live Cell Imaging Facility, Snyder Institute for Chronic Diseases, University of Calgary.
6 Quantalux TM scmos Cameras Our Quantalux scmos cameras are the ideal camera for applications such as fluorescence microscopy due to their low read noise and high dynamic range. Below is an analysis of how these superior specifications lead to better images, and thus better quantitative results, at low light levels. High Sensitivity with <1 e - Read Noise Quantalux scmos cameras have significantly lower read noise, with comparable quantum efficiency, when compared to the CCD sensors used in more conventional scientific cameras. The relative impact of read noise on quantitative measurements will depend upon its contribution to the total noise, relative to the signal-dependent photon shot noise. A measure of camera performance under different noise levels is the Signal-to-Noise Ratio (SNR). The plot below and to the left shows the SNR for our Quantalux camera, a typical conventional CCD camera, and an ideal, shot-noise-limited detector (with no read noise and % quantum efficiency). The plot below and to the right shows the same results normalized to the SNR of an ideal detector under the same conditions. As can be seen in the plots, our Quantalux camera produces images with higher SNR at lower photon levels. Quantalux cameras can be expected to yield higher SNR images than a conventional CCD camera under conditions in which only approximately to photons/pixel are expected to be captured in a given exposure. The specifications of Quantalux cameras make them an ideal choice for a wide range of fluorescence microscope imaging needs and other low-light applications. Quantalux scmos Camera Merged Three-Channel Fluorescence Image of FluoCells Prepared Slide of BPAE Cells Acquired with our Monochrome Quantalux Camera Epifluorescence TIRF Spinning Disk Confocal Single Molecule SNR Comparison SNR Relative to Ideal Detector Epifluorescence TIRF Spinning Disk Confocal Single Molecule 1. SNR Ideal Detector 1 Conventional CCD Quantalux scmos Threshold: SNR=3.1 1 Signal (Photons/Pixel) Relative SNR Ideal Detector Conventional CCD Quantalux scmos. 1 Signal (Photons/Pixel) SNR vs. Input Signal Level for an ideal detector, Quantalux scmos, and conventional scientific CCD cameras. The bars above the plot show photon/pixel counts typically needed for different imaging modalities. SNR relative to an ideal detector vs. Input Signal Level for Quantalux scmos and conventional scientific CCD cameras. The bars above the plot show photon/pixel counts typically needed for different imaging modalities.
7 High 87 db Dynamic Range for Capturing Dim Details and Bright Features A common problem in fluorescence microscopy involves imaging bright fluorescent features in the presence of a dim background. If the camera doesn t have a large enough dynamic range, saturation and floor limitations will limit the simultaneous capture of both bright and dim features. With the Quantalux camera s high dynamic range of 87 db, a single exposure gathers sufficient contrast of bright, moderate, and dim objects at once. The images below show how a single high dynamic range image can be analyzed and visualized to highlight features over any one of these brightness ranges. Figure 1: The Contrast Dialog Window. These settings were adjusted to process the images shown in Figures 2 through 3c. The Contrast settings can also be adjusted during live imaging. Figure 3a: The image contrast from Figure 2 has been adjusted to highlight the amyloid plaque core details. The background, including fibrils and cells, is hidden from view. Figure 3b: The contrast from Figure 2 has been adjusted to highlight the fibrils in the sample, resulting in saturation of the plaque core details in the original image. Figure 2: ThorCam screenshot of an unprocessed image taken using our monochrome Quantalux scmos camera. The sample is a ~ μm thick slice of 5xFAD mouse with amyloid plaques stained with Thioflavin S. The image was acquired at 65 ms exposure using a X,.75 NA immersion objective with water, corrected for the #1.5 coverslip used. Images are courtesy of Craig Brideau, University of Calgary, Alberta, Canada. Figure 3c: Maximizing the background contrast shows nearby cell bodies and nuclei for cytometry; however, the contrast adjustment saturates both fibrils and the plaque cores.
8 Monochrome Camera Quantum Efficiency Plots 7 Quantalux scmos Camera Fast Frame Rate CCD Cameras Standard Cameras UV Enhanced Cameras Megapixel CCD Cameras Standard Mode NIR Enhanced (Boost) Mode Megapixel CCD Cameras Megapixel CCD Cameras Quantalux scmos Camera Housing Dimensions 2.38" (6.3 mm) 1.18" (3. mm).75"-.68" (19. mm mm) Adjustable Lens Mount.76" (19.2 mm).62" (15.8 mm) 1.18" (3. mm).94" (23.8 mm).94" (23.8 mm) 1.19" (3.2 mm) 1." - 32 C-Mount 4- Tapped Hole For ER Cage Rods (4 Places).25" (6.3 mm) 1.19" (3.2 mm)
9 Color Camera Quantum Efficiency Plots Quantalux scmos Color Camera (Available Late 17) Red Green Blue Relative Sensitivity Megapixel CCD Color Cameras Red Green Blue Megapixel CCD Color Cameras Red Green Blue Megapixel CCD Color Cameras Red Green Blue CCD Camera Housing Dimensions.32" (8. mm) 1.79" (45.5 mm) 1.17" (29.7 mm) 2.61" (66.4 mm) 1.63" (41.3 mm) 1.95" (49.5 mm) 3.25" (82.6 mm) 2.36" (6 mm) C-Mount (1."-32) (Back Focus Adjustable) 1/4"- Tapped Hole (4 Places) 3.9" (99.1 mm) 2.36" (6 mm) C-Mount (1."-32) (Back Focus Adjustable) 1/4"- Tapped Hole (4 Places) 3.25" (82.6 mm) 3.9" (99.1 mm) 2.36" (6 mm) 2.36" (6 mm) 4- Tap for 6 mm Cage System (4 Places) Non-Cooled Camera 4- Tap for 6 mm Cage System (4 Places) Hermetically Sealed Cooled Camera
10 Specifications Quantalux 2.1 Megapixel Monochrome and Color a scmos Fast Frame Rate, VGA Monochrome CCD Cameras 1.4 Megapixel Monochrome and Color CCD Cameras 4 Megapixel Monochrome and Color CCD Cameras 8 Megapixel Monochrome and Color CCD Cameras Item # Prefix CSM and CSC 3M and 3UV 151M and 151C 7M and 7C 851M and 851C Effective Number of Pixels (H x V) 19 x 8 6 x x 48 x x 2472 Pixel Size 5.4 x 5.4 µm 7.4 x 7.4 µm 6.45 x 6.45 µm 7.4 x 7.4 µm 5.5 x 5.5 µm Optical Format 2/3 (11 mm Diagonal) 1/3 (5.92 mm Diagonal) 2/3 (11 mm Diagonal) 4/3 (21.4 mm Diagonal) 4/3 (22 mm Diagonal) Max Frame Rate (Full Sensor) 5 fps.7 fps (at MHz Dual-Tap Readout) 23 fps (at MHz Single- Tap Readout) 25.8 fps (at MHz Quad- Tap Readout) b 17.1 fps (at MHz Quad- Tap Readout) c Quantum Efficiency See Plots on Previous Page Read Noise d <1 e - Median <15 e - at MHz <6 e - at MHz (TE Cooled Models) <7 e - at MHz (Standard Models) <12 e - at MHz < e - at MHz Maximum Digital Output 16 Bit 14 Bit e 14 Bit 14 Bit e 14 Bit e Vertical and Horizontal Binning f 1 to 16 1 to 24 1 to 24 1 to 1 to Region of Interest (Min) 8 x 2 Pixels g 1 x 1 Pixels 1 x 1 Pixels 1 x 1 Pixels 1 x 1 Pixels Cooling Passive Thermal Management None TE-Cooled Versions Available TE-Cooled Versions Available TE-Cooled Versions Available Host PC Interfaces Available USB 3. Gigabit Ethernet USB 3. Camera Link Gigabit Ethernet USB 3. Camera Link Gigabit Ethernet USB 3. Camera Link Gigabit Ethernet USB 3. Camera Link Lens Mount SM1 (1.35 -) with Factory-Installed C-Mount Adapter C-Mount (1. -32) C-Mount (1. -32) C-Mount (1. -32) C-Mount (1. -32) a. Color Quantalux Available Late 17 b. Limited to 13 fps at MHz dual-tap readout for Gigabit Ethernet cameras; quad-tap readout is unavailable for GigE cameras. c. Limited to 8.5 fps at MHz dual-tap readout for Gigabit Ethernet cameras; quad-tap readout is unavailable for GigE cameras. d. CCD camera read noise is specified as RMS. For scmos, this is the median read noise as specified by the sensor's manufacturer; RMS read noise is <1.5 e-. e. Gigabit Ethernet cameras operating in dual-tap readout mode are limited to 12 bit digital output. f. Continuous integer values. For CCD cameras, the camera frame rate is impacted by the vertical hardware binning parameter, not the horizontal software binning parameter. For color cameras, when the Image Type setting in ThorCam is anything other than Unprocessed only 1 x 1 binning is available. When set to unprocessed, the camera can bin, but the image produced will be monochrome. g. When Binning at 1 x 1
11 Software ThorCam is powerful software for 32- and 64-bit Windows 7, 8.1, or systems. This easy-to-use graphical interface communicates with the camera to provide system control, image acquisition, and play back. Single-image capture and image sequences are supported. Application programming interfaces (APIs) and a software development kit (SDK) are included for the development of custom applications by OEMs and developers. Support for third-party software packages, such as LabVIEW, MATLAB, µmanager/ ImageJ, and MetaMorph, is provided. ThorCam Software GUI ThorCam Features u Image Acquisition and Review u Measurement and Annotation Draw Lines, Circles, Rectangles, and Freehand Shapes Enter Text Annotations Distance Measurements Line Profile Displays Pixel Value Pixel Peek Shows Numerical Values for Specific Pixels Histogram of Image Data u Timed Series and Review of Image Series A screenshot of the ThorCam software showing some of the analysis and annotation features. The Tally function was used to mark four locations in the image. The line to the lower left was added using the measurement function, with the distance between the points in pixels displayed just above it. At the top of the image a text annotation was added with the experimental details.
12 Custom Cameras In addition to our large selection of standard scientific cameras, we have the capability of building custom cameras designed for unique scientific applications. Options include high-performance board-level cameras, custom camera housings, and software. If you have special requirements, a custom application, or general questions about our capabilities, please contact us at ScientificImaging@thorlabs.com. We can help you evaluate your application and budgetary requirements to create custom cameras to satisfy your needs. Board-level photograph of the sensor and electronics in one of our scientific cameras. Our engineering team simplifies the customization process by following the two-step process below. A scientific camera can be customized by using one of our existing standard cameras as a starting point, or it can be built to the exact needs of an application. Small changes made to our existing designs can be done efficiently. Our electronics boards can be easily reconfigured to fit various housing types. Step 1: Analyze Your Custom Requirements Imaging Specifications u Sensitivity u Wavelength System Requirements u Operating Environment u Space Constraints Application Space u Compliance Issues u Future Developments USA Thorlabs Imaging Systems Phone: Thorlabs Scientific Imaging (TSI) Phone: Thorlabs Quantum Electronics (TQE) Phone: Thorlabs Ultrafast Optoelectronics (UFO) Phone: u Resolution u Speed u Interfaces u Software u Logistics Popular Fluorophore Emission Wavelengths Overlaid with the Quantum Efficiency Curves for our 1.4 Megapixel Cameras Thorlabs Vytran Division Phone: CANADA Thorlabs Canada (ULC) Phone: UK Phone: +44 () Thorlabs Vytran Europe Phone: +44 () Step 2: Configure a Solution Imager Options u UV, Visible, or NIR Spectrum, 4 Megapixel, 8 Megapixel, or Fast Frame Rate CCD u CMOS or scmos I/O Options u Camera Link u Gigabit Ethernet u USB 3. Camera Body Options u Standard Non-Cooled u Hermetically Sealed with Two-Stage TEC u Private Labeling Electronics Modifications u Customized Firmware u Application-Specific Timing and Triggering Modes FRANCE Phone : +33 () GERMANY Thorlabs GmbH Phone: +49 () Thorlabs GmbH (Lübeck) Phone: +49 () Thorlabs Elliptec GmbH Phone: Optics Mounting Options u C-Mount Threading is Standard Software u Initial Evaluation Using ThorCam GUI for Cameras u Algorithm Development Using Popular Third-Party Support Such as MATLAB, µmanager/imagej, LabVIEW, and Metamorph u API / SDK Provided for Software Developers and OEMs Supply Chain u Kanban Stocking Agreements 56 Sparta Avenue Newton, New Jersey 786 Sales: Fax: CHINA Phone: +86 () SWEDEN Phone: Polish Direct Line: JAPAN Phone: BRAZIL Phone: +55 (16)
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