TissueFAXS SL Confocal high throughput configuration (actual appearance of the product may differ)

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2 TISSUEFAXS CONFOCAL TissueFAXS SL Confocal high throughput configuration (actual appearance of the product may differ) TissueFAXS Confocal provides a unique combination of digital slide scanning and laser free fast confocal imaging. It integrates an efficient spinning disc system with TissueFAXS capabilities, cameras and the high speed/high power Spectra X or Spectra 3 light engine. Fascinating range of imaging options Available in all TissueFAXS system configurations TissueFAXS (upright) TissueFAXS i (inverted) TissueFAXS SL slide loader, upright Confocal upgrade for existing TissueFAXS systems Confocal Imager Upgrade 4 or 5 MP camera Filters Spectra X light engine TissueFAXS Confocal scanning software 2

3 More Features Intuitive control for fast operation and minimal training High contrast, high dynamic range for outstanding results even with thick samples Brightfield and fluorescence shading correction Focus plane for reliable scan results Shading correction computed or acquired for/from a specific slide/region to entire/different experiment High dynamic range with 16 bit scanning allows for simultaneous imaging of brighter and darker areas Sample label reading, Tissue Microarray scanning, Z-stacking and Extended Focus images Viewing each z-section or scrolling through an entire z-stack of complete samples, in single channel or full colour overlay Export function for single fields of view or entire region with or without shading correction image Export to different file formats, 8 or 16 bit Plug-in for linking to analysis software TissueFAXS Confocal Performance The system permits fast large scale confocal imaging, the typical performance is about 1,5 hours scanning time for samples with an area of about 70 mm 2 with 4 channels and a z-stack with 13 optical slices. Image courtesy of Dr. Teng-Leong Chew, Light Microscopy Facility, Janelia Research Campus 3

4 Crest Optics Confocal Imager Single pattern spinning disc for large field of view Double pattern spinning disc with pinholes and/or slits Acquisition modes: wide field fluorescence (with fast motorized disc out function), confocal (single or double pattern) Detector field of view: 12mm x 12mm maximum field of view in double pattern configuration Up to 22mm diagonal field of view in single pattern configuration Special disc pattern design for high confocal resolution, improved out-of-focus rejection and higher signal to noise ratio Confocal resolution with 63x NA 1.4 oil immersion objective: < 800nm Pinhole sizes 40, 60, 70 micron or custom pattern Diffraction-limiting design across all the field of view from high magnification-high NA objectives to low magnification/low numerical aperture objectives rpm disc rotation speed Excitation gimbal mount for easy alignment on custom microscope setup and for best signal to noise ratio Motorized dichroic 5 position filter wheel Standard 8 positions motorized emission wheel Plug-in functionality for additional disc Extraction tools for easy insertion and removal of both dichroic filter and emission filter Adapter for LED optic fiber excitation TissueFAXS Confocal Cameras and Filters The TissueFAXS PLUS Confocal cameras provide the system with a large part of its flexibility. The Zyla 5.5, five MP, 16 bit scmos USB camera system is used for spinning disc confocal and wide field fluorescence scanning (using the spinning disc retracted mode). The optional top of the line Hamamatsu Orca FLASH 4.0 V2 scmos cameras provides high speed multi-fluorescence scanning at full 4K resolution. On top of that, the camera has high quantum efficiency (82 % peak QE) and an excellent high signal to noise ratio as well as high contrast. Finally, the Pixelink M4 colour camera provides very fast brightfield scanning (TissueFAXS PLUS configurations). Fluorescence filter cubes are housed both in the spinning disc device (8 positions) and in the systems microscope main filter wheel (10 positions). 4

5 Zyla 5.5 Orca FLASH Pixelink M4 By using one or more multiband filter cubes in one or more of the filter turrets positions the high switching speed of the Spectra X light engine can be fully capitalized on as the filter turret will not have to change filter positions (or less often in case several multibands are used). Lightning Bolt Light Sources Brightfield light is provided by a VIS-LED with hours of life. Spectra X with comparison of its output intensity with Metal Halide and Xenon light sources VIS LED A Spectra X light engine is used for fluorescence illumination. This illumination system combines very high output, necessary for confocality, with very short switching times (< 10 ns rise and fall times between bands). The Spectra X uses six wide band solid state light sources with a fixed configuration, covering most of the spectrum. The user can change the emission filter paddles for the emission bandwidth needed. 5

6 Near Infra Red option Spectra 3 light engine with eight solid state light sources TissueFAXS I PLUS inverted confocal system with 4 or 8 slight stage TissueGnostics Headquarter Distributed by: Taborstrasse 10/2/8 ACCELA s.r.o. A-1020 Vienna, Austria Služeb 4, Prague 10, Czech Republic Tel: office@tissuegnostics.com accela@accela.eu TG Confocal brochure, Revision 2.0, Published & Copyright 2017 TissueGnostics GmbH 6

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