Chemlux Imager. Applications

Transcription

1 Features: High Resolution and High Sensitivity of 6 M Cooled CCD Camera 16-bit, High Dynamic Range, up to 6 X 6 Bin Mode Three-level Movable Platform with Auto Focus Feature Adjustable RGB Light* Intensity to Get Optimized Images %# Cooling Status Indicator on the UI Auto Back Up to Prevent Important Data Loss Remote Operation via LAN/WLAN Login *: for Chemlux 600 RGBW model %: for Chemlux 600 BW model, Epi blue light is available. #: for Chemlux 600 BW, blue light intensity is adjustable. Applications Stain Observation with different lights and filters No Light/Cooled CCD/long exposure Chemiluminescence ECL Blots, ECL ELISA Plates RGB Light* % Multiplex Fluorescence Gels/Blots Protein SDS PAGE UV Light $ Fluorescence DNA/Protein Gels DNA Gel Electrophoresis The Chemlux Imager White Light Colorimetric Gels/Blots, Observation of Colony Plates, ELISA Plates, Autoradiographs, and more. The Chemlux Imager is a powerful, easy-to-use, full-featured and standalone imaging system for fluorescent* % /luminescent/colorimetric signal acquisition from gels or blots via our advanced lighting/imaging technology and proprietary integrated software. Equipped with a 6 M cooled CCD camera along with an absolute sealed darkroom, the system is able to capture the chemiluminescent signal from ECL Western blots for up to 90 min. The system also houses various light sources to allow imaging of proteomic 2D DIGE and to capture fluorescent signals excited by different light sources such as UV $, red*, green*, blue* % and white light. Chemlux s unique UI design allows the user to intuitively obtain image data without any difficulty. The Chemlux Imager is an advanced gel documentation system designed to fully meet all your gel/blot imaging needs. *: for Chemlux 600 RGBW model %: for Chemlux 600 BW model, Epi blue light is available. $: equipped on Chemlux 600 UV Series only

2 Chemiluminescence Applications Qualitative Measurement (Long Exposure Time) Quantitative Measurement (ng- fg Level ECL treatment) 7.5 ug 58.6 ng Chemiluminescence ECL Detection Exposure time: 30 min No noise was observed after 30 min exposure time. Arrow indicates a 120 ng of protein sample. THP1 cell line sample starting from 15 ug to 120 ng (5X in serial dilution) were prepared and loaded for. Proteins were transferred onto nitrocellulose membrane for further analysis. ECL substrate kit was applied on the membrane. Chemiluminescence ECL + (ng-fg level) Detection Exposure time: 3 min Arrow indicated a 58.6 ng of protein sample. THP1 cell line sample starting from 7.5 ug to 58.6 ng (2X in serial dilution) were prepared and loaded for. Proteins were transferred onto nitrocellulose membrane for further analysis. ECL+ (ng-fg level) kit was applied on the membrane. Colorimetric Applications HRP/DAB Treatment Exposure time: 30 ms Epi White Light Setting Coomassie Blue Treatment Exposure time: 30 ms White Backlight Setting

3 Fluorescence Applications (DNA-based) UV $ Epi Blue Detection* % Signal of 0.5 ng DNA was observed. (Arrow pointed) Signal of 0.5 ng DNA was observed. (Arrow pointed) DNA Agarose Gel EtBr Stain UV light Excitation Lower Level Fluorescence Applications (Protein-based)* % Epi Blue Detection* % Epi Green Detection* DNA Agarose Gel ECO Safe Stain Epi Blue Light Excitation Epi Red Detection* Signal of 9 ng protein was Alexa 488 Stain Epi Blue Light Excitation 5000 ms $: Equipped on Chemlux 600 UV Series only Signal of 9 ng protein was Alexa 546 Stain Epi Green Light Excitation 2000 ms Signal of 18 ng protein was Alexa 647 Stain Epi Red Light Excitation

4 Chemlux 600 Series 10.4" Touch LCD Panel EPI RGB Light Sources* % Absolute Sealed Darkroom EPI White Light Movable 3-level Observation Platform

5 Specifications Chemlux 600 W Chemlux 600 BW % Chemlux 600 RGBW* Display 10.4" Multi-touch LCD Panel 10.4" Multi-touch LCD Panel 10.4" Multi-touch LCD Panel Control Panel Touch Panel Cap Sense Touch Panel Cap Sense Touch Panel Cap Sense Touch Panel OS Embedded Linux Embedded Linux Embedded Linux Sensor 6M CCD 6M CCD 6M CCD Resolution 2,736 X 2,160 Pixels 2,736 X 2,160 Pixels 2,736 X 2,160 Pixels Camera Pixel Size 4.54 mm x 4.54 mm 4.54 mm x 4.54 mm 4.54 mm x 4.54 mm Exposure time 10 ms 90 min 10 ms 90 min 10 ms 90 min Cooling Temperature C from Ambient C from Ambient C from Ambient Lens 1" 25 mm Fixed Focal Length 1" 25 mm Fixed Focal Length 1" 25 mm Fixed Focal Length Auto Focus Yes (Auto) Yes (Auto) Yes (Auto) IRIS F0.95 F0.95 F0.95 Optical Zoom Filter 6 Position (Motor-powered) 6 Position (Motor-powered) 6 Position (Motor-powered) Viewing Area 26 cm x 20 cm (Maximum) 26 cm x 20 cm (Maximum) 26 cm x 20 cm (Maximum) (For Chemlux 600 W-UV only) (For Chemlux 600 BW-UV only) (For Chemlux 600 RGBW-UV only) Light Source White Backlight Plate Yes, 26 cm x 20 cm Yes, 26 cm x 20 cm Yes, 26 cm x 20 cm EPI RGB N/A EPI Blue light EPI R/G/B light EPI White Light Yes Yes Yes Lift Plate 3 Lift Level Manual Adjustable Manual Adjustable Manual Adjustable USB 2.0 Port X3 (Front x1; Rear x2) X3 (Front x1; Rear x2) X3 (Front x1; Rear x2) Connection 10/100 Ethernet Port X1 X1 X1 N/A EPI R/G/B light Capture Mode EPI blue Chemiluminescent (Dark Chemiluminescent (Dark Capture Chemiluminescent (Dark Room) Room) Room) Image Format TIFF 16 Bit/ JPG/BMP TIFF 16 Bit/ JPG/BMP TIFF 16 Bit/ JPG/BMP Storage 8 GB Data storage 8 GB Data storage 8 GB Data storage Certificates CE & FCC CE & FCC Certified CE & FCC Certified CE & FCC Certified Dimensions L x W x H 452 X 423 X 659 mm 452 X 423 X 659 mm 452 X 423 X 659 mm Power Input AC V, 50/60Hz, 200W AC V, 50/60Hz, 200W AC V, 50/60Hz, 200W Weight Net Weight 52 Kg (Approximately) 52 Kg (Approximately) 52 Kg : UV illuminator is equipped with Chemlux 600 UV Series models (Chemlux 600 W-UV, Chemlux 600 BW-UV, and Chemlux 600 RGBW-UV) only. Chemlux 600 Series does not equip UV illuminator and is non-upgradable for UV illumination after purchase.