GelDoc-It TS2 & ChemiDoc-It TS2 Imagers

Transcription

1 GelDoc-It TS2 & ChemiDoc-It TS2 Imagers Installation and User Instructions UVP, LLC Ultra-Violet Products Ltd W. 11th Street Unit 1, Trinity Hall Farm Estate Upland, CA Nuffield Road, Cambridge CB4 1TG UK Phone: (800) Phone: +44(0) Fax: (909) Fax: +44(0) Web Site: Rev A

2 GelDoc-It TS2 and ChemiDoc-It TS2 Imagers 2 Table of Contents Introduction... 3 System Components... 4 Optional Equipment... 7 Setup Instructions... 9 Components... 9 Installing Emission Filters Using the System Powering Up the System Operating the TS2 Software Interface Selecting Emission Filters Using the Transilluminator Using the Overhead (Epi) Lighting Using the UV Gel Viewer Window Image Focusing Service Procedures Return Procedure Replacement Parts and Accessories Troubleshooting Technical Support Warranty... 15

3 GelDoc-It TS2 and ChemiDoc-It TS2 Imagers 3 Introduction The GelDoc-It TS2 Imager is a high resolution imager capable of capturing, documenting and analyzing fluorescent gel images. In addition to gel documentation capabilities, the ChemiDoc-It TS2 Imager is equipped with a cooled, scientific-grade CCD camera allowing users to capture, document and analyze chemiluminescent blot images, including Western blots. The GelDoc-It TS2 and ChemiDoc-It TS2 are self-contained imaging systems with a built-in 15.6 touch screen and computer. Additionally, the systems incorporate a light-tight darkroom with TS2 Software for image acquisition. The darkroom has a UV-blocking gel viewer window, built-in overhead epi white lighting, a UV transilluminator and a five-position slide-out emission filter wheel with an ethidium bromide (EtBr) emission filter included as standard. Save images to the internal hard drive, to an external USB storage device or to a network drive for later quantitative analysis or enhancement for publication.

4 GelDoc-It TS2 and ChemiDoc-It TS2 Imagers 4 System Components Refer to the packing slip and pictured components for specific parts and components included with the system LCD Touch Screen with Built-In Computer Five Position Slide- Out Emission Filter Wheel Main Power Button Built-In Overhead White Lights UV Safety Interlock Switch & Override Black Chemi Tray Transilluminator on Roll-Out Tray Stylus with Adhesive Holder (Not Shown) Power Indicator Lights Camera and Lens Built Into Darkroom Hood BioLite Access Port USB and SD Card Access Port Wide Access Door UV Gel Viewer Window

5 GelDoc-It TS2 and ChemiDoc-It TS2 Imagers 5 Specifications Power Requirements: 100/115V, 50/60Hz; 3.1 Amps at 120 Volts 230V, 50/60Hz; 1.55 Amps at 230 Volts Mains supply voltage fluctuations are not to exceed 10 percent of the nominal supply voltage Pollution Degree: 2 Installation Category: Altitude: II Up to 2000m Ambient Temperature: 5 C to 40 C Humidity: Maximum relative humidity of 80% for temperatures up to 31 C, decreasing linearly to 50% maximum relative humidity at 40 C Built-In Touch Screen Computer Operating System: Connectivity Ports: Wireless Networking Capability: USB Flash Drive Capacity: Internal Hard Drive: Software: Windows 7, 64-Bit 2 USB, 1 SD/SDHC/MMC (side of system) 4 USB, VGA and Ethernet (rear of system) b/g/n 2 GB (minimum) 250 GB (minimum) TS2 (Touch Screen) Software (supplied with all systems) VisionWorks LS Software (supplied with ChemiDoc-It TS2 only) Cameras and Lenses GelDoc-It TS2 : The GelDoc-It TS2 is equipped with the GelCam 310 camera, a scientific-grade monochrome CCD camera with a resolution of 2.0MP (1600x1200). The GelCam 310 is equipped with a mm f/1.2 zoom lens. ChemiDoc-It TS2 : The ChemiDoc-It TS2 is equipped with either the Chemi 415 or MegaCam 810 scientific-grade monochrome CCD camera. Both cameras are Peltier cooled and offer full 16-bit file bit depth: The Chemi 415 camera is a 1.4MP unit (1360x1024) with Peltier cooling to 35 C below ambient. The Chemi 415 has a peak quantum efficiency of 62% and is capable of binning from 1x1 to 8x8. This camera is equipped with a mm f/1.2 zoom lens. The MegaCam 810 camera is an 8.1 MP camera (3296x2472) with Peltier cooling to 35 C below ambient. The MegaCam 810 has a peak quantum efficiency of 50% and is also capable of binning from 1x1 to 8x8. This camera is equipped with either a 50mm f/1.2 lens or a 30mm f/1.4 lens, both of which have a fixed focal length (non-zoom capable). All camera settings are factory pre-set for optimum performance when viewing gels, films or membranes under low light level conditions. Contact UVP Technical Support prior to making any adjustments to the camera settings. Ethidium Bromide (EtBr) Emission Filter The ethidium bromide (50mm 2 ) UV-blocking bandpass interference filter blocks UV and IR radiation emitted from the transilluminator. The filter is placed in the slide-out filter wheel assembly on the side of the Imager. The filter allows visualization of fluorophores from nm, targeting the ethidium bromide emission peak of 605nm.

6 GelDoc-It TS2 and ChemiDoc-It TS2 Imagers 6 Additional filters are available for other specific fluorophores, including custom filters. Filters can also be removed when imaging non-fluorescent media (including chemiluminescent blots, protein gels, colony plates, etc.) in order to produce brighter images. Contact UVP for ordering information. Darkroom The darkroom is light tight to provide optimal imaging conditions. Darkroom features include: Epi (overhead) LED white light Roll-out transilluminator tray UV-safe gel viewer window built into the darkroom door Five position emission filter wheel Brackets and built-in power ports for installing optional epi UV illumination lamps Brackets for installing optional high-intensity halogen or xenon BioLite epi light guides Built-in power port for transilluminator Power cord for optional LED White Light Plate UV safety interlock switch with manual override to disable UV transillumination and optional UV epi illumination when darkroom door is opened Transilluminator The GelDoc-It TS2 and ChemiDoc-It TS2 Imagers can accommodate UVP s Benchtop and FirstLight transilluminator models. UVP offers a variety of transilluminator configurations, including Benchtop models with multiple wavelengths and variable intensities as well as the highly uniform, patented FirstLight transilluminator. Refer to the packing slip for the transilluminator included with your system. Note: For UV protection and to extend the life of the UV transilluminator, the system incorporates a customizable transilluminator shutoff timer built into the software. For additional information, refer to the TS2 Software User Manual included with the system or available online at LCD Touch Screen The GelDoc-It TS2 and ChemiDoc-It TS2 Imagers contain a fully integrated 15.6-inch color touch screen. The touch screen allows the user to perform a variety of tasks, including previewing, capturing, saving and printing images, as well as selecting preference options, without the use of an external mouse or keyboard. The screen can be pressed by finger or with the included pen-shaped stylus. For users who prefer not to use the touch screen interface, an external keyboard and mouse are included as standard. These components can be plugged into any available system USB port. TS2 Software Image analysis and hardware functions for the GelDoc-It TS2 and ChemiDoc-It TS2 Imagers are controlled by the TS2 (Touch Screen) Software interface. In addition to image preview, capture and save functions, the TS2 Software controls camera and lens functions (exposure, aperture, focus and zoom), image printing, lighting and filter selection, and other user preferences. For additional information, refer to the TS2 Software User Manual included with the system or available online at TS2 Software Screen Fluorescent Focus Target The UVP Fluorescent Focus Target fluoresces when placed on a UV transilluminator or when exposed to overhead UV. The Target provides sharp fluorescent images to aid in adjusting the lens and camera settings for ideal imaging results. Fluorescent Focus Target

7 GelDoc-It TS2 and ChemiDoc-It TS2 Imagers 7 Optional Equipment UVP offers a variety of optional equipment to support the needs of varying laboratory environments. Refer to Replacement Parts and Accessories at the end of this manual for optional equipment part numbers. Thermal Printer The thermal printer provides archive quality, 256 grayscale prints and five optional cost-effective print sizes. Thermal Printer UV Handheld Lamps for Epi (Overhead) Lighting A set of two 4-watt ultraviolet lamps can be connected inside the darkroom to provide epi UV illumination. These lamps can be switched on or off in the Lighting and Filters menu within the TS2 Software and can also be removed from the darkroom and used as standalone handheld lamps (as seen in the image to the right). UV Lamp LED White Light Plate The LED White Light Plate emits high uniformity with less than 5% coefficient of variance (CV). Plug the LED White Light Plate directly into the power supply within the system darkroom and select White Light under the Lighting and Filters menu within the TS2 Software. LED White Light Plate Converter Plates An alternate to the LED White Light Plate, the UV/White Converter Plate allows imaging of non-fluorescent stained media with an ultraviolet transilluminator. The converter plate is specially coated to convert 302nm UV output to white light rather than using a separate white light box or plate. The Visi-Blue Converter Plate (not shown) converts UV to a safe nm wavelength designed for use with blue excitation samples and SYBR Green, SYPRO Orange and GFP stains. UV/White Converter Plate

8 GelDoc-It TS2 and ChemiDoc-It TS2 Imagers 8 VisionWorks LS Analysis Software The GelDoc-It TS2 can be configured with VisionWorksLS Software for analysis of gels, plates and membranes. The software features image enhancement, complete analysis tools and reporting capabilities, and is ideal for users who require image analysis functions in addition to the standard image acquisition capabilities of the GelDoc-It TS2. NOTE: VisionWorksLS is included as standard with the ChemiDoc-It TS2.

9 GelDoc-It TS2 and ChemiDoc-It TS2 Imagers 9 Setup Instructions Components When unpacking the GelDoc-It TS2 and ChemiDoc-It TS2, the following items will be included: 1. GelDoc-It TS2 or ChemiDoc-It TS2 darkroom 2. Ethidium bromide (EtBr) emission filter 3. Transilluminator 4. Power cable 5. Keyboard and mouse 6. VisionWorksLS Software (included with ChemiDoc-It TS2 only) 7. Supporting documentation When unpacking and setting up the darkroom, two people are required to move the darkroom. Place the darkroom on a flat surface which can provide adequate support for up to 100 pounds. WARNING: Do not attempt to perform any setup procedures while the system is plugged in or powered on unless otherwise instructed. CAUTION: Do not install the system in areas with high moisture, dust or high temperatures. Keep the equipment away from motors or any other large magnetic equipment apparatus. This system is designed for indoor use only. Connecting the Power Cables 1. Plug the main power cable into the back of the darkroom and the other end into a surgeprotected power outlet. Note: It is recommended to leave the power switch on the back of the system in the ON position except when the system will not be used for an extended period of time (one day or longer). Note: Do not position the system so that it is difficult to access the power cable and operate the main power switch at the back of the unit. 2. Inside the darkroom, place the transilluminator on the roll-out tray. Connect the transilluminator to the internal power jumper cable. 3. If installing the LED White Light Plate, place the plate on top of the UV transilluminator and connect the power cord coming from inside the darkroom to the back of the Plate. 4. If installing epi UV handheld lamps, place the lamps in the brackets located at the top of the darkroom. Plug the handheld lamps into the outlets provided inside the darkroom. Place the power switches located on the outside of the handheld lamps in the ON position.

10 GelDoc-It TS2 and ChemiDoc-It TS2 Imagers 10 Installing Emission Filters To install the 50mm 2 ethidium bromide (EtBr) filter and any other emission filters: 1. Carefully remove the filter from the protective plastic case, holding the filter at the edges to prevent placing fingerprints on the glass surface. 2. The filter wheel is located on the user s right side of the darkroom. Press in on the slide-out filter wheel door and the door will pop out slightly. Pull the filter wheel assembly out until it stops. Slide-Out Filter Wheel Door 3. Manually rotate the filter wheel to the desired position. Place the included ethidium bromide filter in Position #1. NOTE: Before placing the filter in the filter wheel, ensure that the text on the edge of the filter is positioned so it is right side up when facing the installer. 4. Note the position of all installed filters for entry into the Lighting and Filters menu in the TS2 Software (refer to the TS2 Software User Manual for more information). 5. Once all filters have been loaded, slide the filter wheel assembly back into the system. Press the filter wheel assembly door until the assembly locks into place. Additional and replacement emission filters are available through UVP. Refer to the Replacement Parts and Accessories section of this manual for ordering information.

11 GelDoc-It TS2 and ChemiDoc-It TS2 Imagers 11 Using the System Powering Up the System Set the black power switch on the back of the system to the ON (I) position. Once plugged in to a wall outlet and the black power switch on the back of the system is placed in the ON (I) position, the GelDoc- It TS2 and ChemiDoc-It TS2 systems are always powered on. Press the POWER button on the front of the system to power on the internal computer/touch screen and access controls for the camera, lenses, lighting and other functions. Power Indicator Lights The Power Indicator Lights on the front of the unit indicate when the system s main power and ultraviolet illumination are on. Main Power will illuminate green when the black main power switch on the back of the unit is in the ON (I) position (this does not indicate that the internal computer or touch screen are on). Ultraviolet On will illuminate red when ultraviolet illumination is Power Button active within the darkroom. The POWER button will illuminate blue when the internal computer and touch screen are on. (Note: The black main power switch on the back of the unit must be in the ON (I) position in order for the internal computer and touch screen to function.) Note: It is recommended to leave the power switch on the back of the system in the ON position except when the system will not be used for an extended period of time (one day or longer). Operating the TS2 Software Interface Upon startup, the internal computer will proceed through the boot-up process. When complete, the screen will display the Windows desktop. The TS2 Software screen, similar to the one below, will automatically open shortly thereafter: To exit the TS2 Software interface, press either the close (X) or minimize (_) buttons at the top right corner of the software (see red circle above). To power down the system, exit the TS2 Software interface by

12 GelDoc-It TS2 and ChemiDoc-It TS2 Imagers 12 pressing either the close (X) or minimize (_) buttons at the top right of the software. Then, using the Windows Start menu, select Shut Down. Refer to the TS2 Software User Manual for further instructions on using the software. Selecting Emission Filters See the Installing Emission Filters section of this manual for instructions on installing individual emission filters. Once all filters have been installed, select the Lighting and Filters menu in the TS2 Software and click the Reset Wheel button under the Filters tab to realign and calibrate the filter wheel within the system. Using the Transilluminator To use the UV transilluminator or LED White Light Plate, ensure that the components are plugged into the system (see the Connecting the Power Cables section of this manual for more information). Using the Lighting and Filters menu in the TS2 Software, select either UV or White Light Plate depending upon the application. When using the UV transilluminator, ensure that the green power switch on the front of the transilluminator unit is in the ON position. NOTE: For UV protection and to extend the life of the UV transilluminator, the system incorporates a customizable transilluminator shutoff timer built into the software. For additional information, refer to the TS2 Software User Manual included with the system or available online at Using the Overhead (Epi) Lighting To operate the built-in overhead white light, use the Lighting and Filters menu in the TS2 Software and select White under Epi Illumination. To operate the optional overhead (epi) UV illumination, ensure that the optional UV handheld lamps are plugged into the back of the darkroom interior and are mounted to the brackets at the top of the darkroom. Also ensure that the power switches, located on the handheld lamps, are in the ON position. To provide power to the handheld lamps, use the Lighting and Filters menu in the TS2 Software and select Accessory under Epi Illumination. Using the UV Gel Viewer Window The UV Gel Viewer Window, built into the darkroom door, allows users to view the interior of the darkroom without opening the darkroom door. To open the Window, press firmly on the top of the Window cover to release the pressure-sensitive clasp and open the viewer. The Window glass is UV blocking while providing a clear view to the transilluminator surface for sample viewing without opening the darkroom door. NOTE: Close the UV Gel Viewer Window prior to capturing a light-sensitive image such as a chemiluminescent blot. Image Focusing Prior to capturing an image, prepare the image focus: 1. Turn on the transilluminator and place the Fluorescent Focus Target (see the Fluorescent Focus Target section of this manual for more information) on the transilluminator surface. NOTE: The darkroom has a UV safety switch that turns off the transilluminator and optional UV handheld lamps when the door is open. 2. Using the TS2 Software, click to turn ON the Live Preview. Adjust the exposure, aperture, zoom (if applicable) and focus controls on the bottom portion of the TS2 Software screen until an ideal image is visible.

13 GelDoc-It TS2 and ChemiDoc-It TS2 Imagers 13 Service Procedures Return Procedure A Returned Goods Authorization (RGA) number must be obtained from UVP Customer Service before returning any product. Replacement Parts and Accessories To order accessories or replacement parts for the GelDoc-It TS2 and ChemiDoc-It TS2 Imagers, contact UVP s offices. Part Description UV Handheld Lamps: UV Handheld Lamp, 254/365nm, 4 watt (115V) UV Handheld Lamp, 254/365nm, 4 watt (230V) Part Number (2 Recommended) (2 Recommended) Fuses: Fuse, 3.15A (for Darkroom) Fuse, 2A (for Transilluminators) (2 Recommended) (2 Recommended) Emission Filters: Filter, Ethidium Bromide, 50mm Square Filter, SYBR Green, 50mm Square Filter, SYBR Gold, 50mm Square Transillumination Accessories: LED White Light Plate White Light Converter Plate, 21x26cm Visi-Blue Converter Plate, 21x26cm Gel Accessories: Gel-Cutter Gel-Ruler Gel-Scooper Gel-Tray, small Gel-Sentry DNA Preparation Plate Fluorescent Standard Step Tablet Protective Equipment: Spectacles, UV Blocking (UVC-303) Goggles, UV Blocking (UVC-503) Faceshield, UV Blocking (UVC-803) Troubleshooting No Power to the Darkroom or Transilluminator 1. Recheck the main power cord connection to the GelDoc-It TS2 /ChemiDoc-It TS2 darkroom as well as the power cables between the darkroom and transilluminator, LED White Light Plate or optional UV handheld lamps. 2. Check the fuses located at the back of the unit next to the power port. A small flat-head screwdriver or similar tool will be required. Push the bottom tab of the fuseholder up until the fuseholder s bottom pops out. Then, push the top tab down until the top pops out. The entire fuseholder can now be pulled out.

14 GelDoc-It TS2 and ChemiDoc-It TS2 Imagers 14 Inspect the thin wire within each glass fuse to see if there is a break in the wire. If so, replace the fuse(s). If fuses are blowing repeatedly, contact UVP Technical Support for additional troubleshooting. Transilluminator Will Not Turn On 1. In addition to turning on UV transillumination in the Lighting and Filters menu within the TS2 Software, the transilluminator itself has a power switch. Make sure that the green transilluminator power switch, located on the front of the transilluminator, is in the ON position. 2. For UV protection and to extend the life of the UV transilluminator, the system incorporates a customizable transilluminator shutoff timer built into the software. For additional information, refer to the TS2 Software User Manual included with the system or available online at Error Messages Appear on the Screen Care and Cleaning 1. An error message that is related to the TS2 software interface or Microsoft Windows may appear on the screen. If the message is related to Microsoft Windows, such as a reminder to activate or update the copy of Windows, please contact your system administrator for assistance. 2. If an error message appears repeatedly and your system administrator does not recognize it as a Microsoft Windows error, contact UVP Technical Support for further assistance. Use only mild soap or detergent solution for cleaning. Do NOT use oil- or petroleum-based cleaners for the cabinet. Ensure that the system is turned OFF and unplugged during cleaning. Technical Support UVP offers free lifetime technical support on all of its products and software. Should you have any questions regarding the product s use, operation or repair, contact UVP s offices at the locations below, or visit If you are in North America, South America, East Asia or Australia: Call (800) or (909) , and ask for Customer Service during regular business days, between 7:00 am and 5:00 pm, PST. your message to: info@uvp.com Fax Customer Service, and send it to (909) Write to: UVP, LLC 2066 W. 11 th Street, Upland, CA USA If you are in Europe, Africa, the Middle East of Western Asia: Call +44(0) , and ask for Customer Service during regular business days between 9:00 am and 5:30 pm. your message to: uvp@uvp.co.uk Fax Customer Service, and send it to: +44(0) Write to: Ultra-Violet Products Ltd Unit 1, Trinity Hall Farm Estate, Nuffield Road, Cambridge CB4 1TG UK

15 GelDoc-It TS2 and ChemiDoc-It TS2 Imagers 15 Warranty UVP's products are guaranteed to be free of defects in materials, workmanship and manufacture for one (1) year from date of purchase. Consumable and disposable parts including, but not limited to tubes and filters, are guaranteed to be free from defects in manufacture and materials for ninety (90) days from date of purchase. Transilluminators carry a two (2) year warranty from date of purchase. If equipment failure or malfunction occurs during the warranty period, UVP shall examine the inoperative equipment and have the option of repairing or replacing any part(s) which, in the judgment of UVP, were originally defective or became so under conditions of normal usage and service. No warranty shall apply to this instrument, or part thereof, that has been subject to accident, negligence, alteration, abuse or misuse by the end-user. Moreover, UVP makes no warranties whatsoever with respect to parts not supplied by UVP or that have been installed, used and/or serviced other than in strict compliance with instructions appearing in this manual. In no event shall UVP be responsible to the end-user for any incidental or consequential damages, whether foreseeable or not, including but not limited to property damage, inability to use equipment, lost business, lost profits, or inconvenience arising out of or connected with the use of instruments produced by UVP. Nor is UVP liable or responsible for any personal injuries occurring as a result of the use, installation and/or servicing of equipment. This warranty does not supersede any statutory rights that may be available in certain countries. GelDoc-It, ChemiDoc-It, VisionWorks and FirstLight are registered trademarks of UVP, LLC. BioLite is a trademark of UVP, LLC.