Leica AF6000 E AF7000

Transcription

1 Leica F6000 E F7000 Leica Microsystems dvanced Widefield Systems Technical Documentation Living up to Life

2 Upgrade Options from Entry-Level to High-End Solutions Main characteristics pplications Includes Leica DM1000 Ergonomic system microscope Leica DM2000 dvanced system microscope Leica DM2500 dvanced system microscope 100 W Leica DM3000 dvanced system microscope, motorized Leica DM4000 utomated upright microscope for biological, laboratory and research applications Leica F6000 E Leica F6000 Leica F6500 Leica F7000 Entry-level fluorescence acquisition system, 2D time-lapse, multichannel overlay Fluorescence imaging and documentation F6000 E SW + P Fluorescence imaging and analysis system, high flexibility (without real-time control) General fluorescence imaging, 3D time-lapse experiments, multi-positioning, deconvolution F6000 SW + P High-speed fluo rescence system, real-time control of camera and one filter wheel Functionality of F6000, plus fast 2D time-lapse and fast ratio imaging, peripheral triggering F6000 SW + P + TR6500 HS Dedicated live cell system, full real-time control TIRF, fast 3D timelapse, Fura2, FRET, deconvolution, peripheral triggering F6000 SW + P + TR7000 HS Leica DM5000 utomated upright microscope for demanding research applications Leica DM5500 utomated upright microscope for high-end research applications with motorized focus Leica DM6000 Fully automated upright microscope for cutting edge biomedical research Leica DMI3000 Manual inverted research microscope Leica DMI4000 utomated inverted research microscope Leica DMI6000 Fully automated inverted research microscope Leica M205 F High-end stereomicroscope Leica MacroFluo High-end macroscope > > > > > > 2

3 Software intuitive and guiding user interface context sensitive online help multi-dimensional data acquisition time-lapse experiments Mark and Find feature in combination with motorized stages (not on F6000 E) quantification tools (not on F6000 E) tile scanning (not on F6000 E) Software options Live Data Mode 3D visualization olocalization 3D Deconvolution 2D Deconvolution FRET SE Dye Finder Well plate acquisition interactive data recording allowing job-sequencing and online evaluation maximum and other projections, simulated fluorescence process, 3D rotation and animations histogram based colocalization and area measurements fully integrated deconvolution algorithm using adaptive calculated or supplied PSF fully integrated 2D deconvolution offering no-neighbour and nearestneighbour algorithm powerful wizard for FRET acquisition and analysis multi-color restoration, channel unmixing predefined and user-definable patterns Hardware Specifications SuperZ Galvo z-positioner travel range 250 µm step size 61 nm Piezo z-positioner travel range 100 µm step size 25 nm Leica EL6000 External light source lamp light intensity life time shutter time HXP 120 W 45 VIS (Osram), Metal Halogenide bulb 3.5 W (with IR filter, at the end of light guide) average 2000 h 6 ms Leica External Filter Wheels Exitation, emission and attenuation filter wheels filter positions 5 switching time 27 ms between adjacent positions 36 ms for maximum position distance Incubator L/Incubator L TIRF temperature min. temperature max. control accuracy step size 3 above room temperature 20 above room temperature +/ 0.1 at sensor 0.1 3

4 ameras Leica DF310 FX amera gain 1 to bit 20 K to ambient 1392 x 1040 pixel pixel size 6.45 µm x 6.45 µm interline progressive scan D color Leica DF345 FX amera gain 1 to bit active Peltier 1600 x 1200 pixel pixel size 4.4 µm x 4.4 µm interline progressive scan D monochrome Leica DF360 FX amera gain 1 to bit 20 K to ambient 1392 x 1040 pixel pixel size 6.45 µm x 6.45 µm interline progressive scan D monochrome Leica DF425 & DF425 amera gain 1 to bit 20 K to ambient (DF425: no ) 2592 x 1944 pixel pixel size 2.78 µm x 2.78 µm interline frame transfer D color no Leica DF495 amera gain 1 to bit 20 K to ambient 3264 x 2448 pixel pixel size 2.7 µm x 2.7 µm interline frame transfer D color no 4

5 ameras Princeton Instruments oolsnap HQ2 amera PI board version gain 1 or 2 12 bit x 1040 pixel pixel size 6.45 µm x 6.45 µm interline progressive scan D monochrome Princeton Instruments ascade II 512 amera gain on chip multiplication gain 16 bit x 512 pixel pixel size 16 µm x 16 µm frame transfer D monochrome, back thinned Hamamatsu amera gain on chip multiplication gain 14 bit x 1000 pixel pixel size 8 µm x 8 µm frame transfer D monochrome Hamamatsu amera gain on chip multiplication gain 16 bit 65 (-80/-90 water cooled) 512 x 512 pixel pixel size 16 µm x 16 µm frame transfer D monochrome, back thinned Hamamatsu Orca R 2 amera gain 1 to 10 12/16 bit 35 (-40 water cooled) 1344 x 1024 pixel pixel size 6.45 μm x 6.45 μm interline progressive scan D monochrome 5

6 ameras ndor ixon 897 amera gain on chip multiplication gain 14 bit 85 air cooled 512 x 512 pixel pixel size 16 µm x 16 µm frame transfer D monochrome, back thinned ndor ixon 885 amera gain on chip multiplication gain 14 bit 70 air cooled 1004 x 1002 pixel pixel size 8 µm x 8 µm frame transfer D monochrome High-Speed omponents for the Leica dvanced Widefield System Line Leica F6000 E Leica F6000 Leica F6500 Leica F7000 Time-lapse Multi-channel overlay amera control Z stack Motorized XY stage Deconvolution External filter wheels 1 FW* 1 FW* 1 FW 4 FW Peripheral triggering 2 In, 1 Out 4 In, 4 Out SuperZ Galvo fine focus Pifoc fine focus TIRF available, high-speed available, non high-speed not available *Leica TR5500, 6000 or 6500 required Leica External Fast Filter Wheel for high-speed excitation, attenuation and emission control. Leica Water Immersion Micro Dispenser supplies water immersion automatically during experiments with living specimens. 6

7 System Overview Leica F6000 E ameras Leica DF360 FX /3, 0.8 dapter Leica DF345 FX /3, 0.63 dapter Leica DF310 FX 1 or 2 Monitors 19 1x x or 2 Monitors 22 1x x x -mount adapter x -mount adapter Recommended ameras Leica DF360 FX Leica DF345 FX Leica DF310 FX Leica DF495 Keyboards Keyboard US Keyboard UK Keyboard Italian Keyboard German Keyboard Spanish Keyboard Swedish Keyboard French Leica DF Leica DF425 M205 F MacroFluo DM1000 DM2000 DM2500 DM3000 DM4000 DM5000 DM5500 DM6000 DMI3000 DMI4000 DMI6000 F6000 E without monitors incl. F6000 E licence and P Upgrade F6000 E to F6000 7

8 Shutter Itensity Shuter Power Shutter Shutter Itensity Itensity Shuter Power Shuter Power System Overview Leica F6000 for M205 F 3h + Leica Ext. FW (TR5500/6000/6500 only) 3h Keyboards Keyboard US Keyboard UK Keyboard Italian Keyboard German Keyboard Spanish Keyboard Swedish Keyboard French limate hamber Upright D 2x Monitor 19 Software Options Live Data Mode D Visualization olocalization D Deconvolution D Deconvolution D 2x Monitor Dye Finder FRET Software (not for M205 F and MacroFluo) ore Review Software w/o Deconvolution Well Plate cquisition (DMI6000 with motorized/scanning stage or M205 F/MacroFluo with Isopro stage) D Shutter cable EL Liquid light guide dapter for M205 F D Shutter cable EL Liquid light guide dapter 1" for EFW 3h dapter 1" Shutter cable EL Liquid light guide SFL4000/SFL Incubator for DM / 230V / 115V Temp control unit Digital ntivibration table System Desk Leica SFL Leica SFL / 230V / 115V Heating control unit / 230V / 115V Temp control unit nalog ameras Leica DF360 FX Stage-S mot condenser holder (requires TR6500) Stage-E7650, rotating Holder for glass slides 3"x1" Slide holder ERGO Single-hand operation E E /3, 0.8 dapter /3, 0.63 dapter Leica DF345 FX Leica DF310 FX Stage-R7625, rotating Slide holder IO I Single-hand operation Microscope Stage-R7650, condenser holder. rotating for 2 specimens Slide holder IO II Oil immersion for 2 specimens E Stage-RL7625, with stage bracket + rotating for 1 specimen Slide holder IO I left DM6000 only: Slide holder IO I Single-hand operation for 1 specimen for mot. stage M205 F MacroFluo DM 5000 DM Stage mot., with stage bracket and condenser holder Slide holder IO II Oil immersion for 2 specimens for mot. stage 8

9 Fast FIM FFIM onnection cable Fura Fura2-set onnection cable Regular fixed stage plate Fixed heating stage plate Fixed stage plate Scanning Stage H117 N1 DM (cannot be combined with Incubator L, requires TR6500) Objective guide for special holder frame Universal mounting frame Stage Inserts Special holder frames Triple Triple-band-set onnection cable FP/YFP FRET Please use filter cube set for FP/YFP FRET 4000 (not available in the US) 7000 (not available in the US) Regular 3-plate stage Plate stage motorized (TR 127x83) dapter cable Scanning stage motors below stage (TR127x83, requires TR6500) dapter cable Pecon inserts and O2 overs Stage insert for microtiter plates O2 cover for stage insert microtiter plates Insert N O2 cover for temp. insert P ell hamber PO-R cell cultivation system Perfusion insert, open with cover for PO-R ameras Leica DF360 FX oolsnap HQ2 PI board version limate hambers Inverted Incubator L ascade II x -mount adapter Hamamatsu EM-D high speed set Hamamatsu EM-D back illuminated / 230V / 230V / 115V / 115V Heating control unit Temp control unit x -mount adapter Hamamatsu Orca R 2 D ndor ixon DU-897 EM D back illuminated ndor ixon DU-885 EM-D high speed set Leica DF345 FX Leica DF310 FX Incubator L TIRF O2 ontroller including Humidifier Leica DF Leica DF Leica DF / 230V / 115V O2 controller with accessories D Water Immersion Micro Dispenser (DMI6000 only) DM 6000 DMI 4000 DMI 6000 F without monitors including F6000 core licence and P Upgrade Kit F6000 to F6500 for DMI4000 : Upgrade Kit F6000 to F7000 for DMI4000 : Upgrade Kit F6000 to F6500: Upgrade Kit F6000 to F7000:

10 Shutter Shutter Itensity Itensity Shuter Power Shuter Power System Overview Leica F6500 Keyboards Keyboard US Keyboard UK Keyboard Italian Keyboard German Keyboard Spanish Keyboard Swedish Keyboard French ameras x -mount adapter x -mount adapter Leica DF360 FX Trigger cable DF ameras for TR Leica DF345 FX Trigger cable DF ameras for TR Leica DF310 FX Trigger cable DF ameras for TR oolsnap HQ2 PI board version Trigger cable Roper cameras for TR ascade II Trigger cable Roper cameras for TR Hamamatsu EM-D high speed set Trigger cable Hamamatsu cameras for TR Hamamatsu EM-D back illuminated Trigger cable Hamamatsu cameras for TR Hamamatsu Orca R 2 D Trigger cable Hamamatsu Orca R 2 for TR ndor ixon DU-897 EM D back illuminated Trigger cable ndor cameras for TR7000 Peripheral triggering 2 channel in and 1 out dapter peripheral triggering 3h 2x Monitor 19 2x Monitor 22 limate hamber Upright ndor ixon DU-885 EM-D high speed set Trigger cable ndor cameras for TR High Speed shutter cable Liquid light guide dapter 1" + Leica External FW Software Options 3h Incubator for DM / 230V / 115V Heating control unit / 230V / 115V Temp control unit Digital / 230V / 115V Temp control unit nalog Live Data Mode D Visualization olocalization D Deconvolution D Deconvolution Dye Finder FRET Software ore Review Software w/o Deconvolution Well Plate cquisition (DMI6000 only, requires motorized/scanning stage) System Desk dapter 1" SFL High Speed S Liquid light g Holder for two FW sliders Le Stage-S mot condenser holder Stage-E7650, rotating Holder for glass slides 3"x1" Slide holder ERGO Single-hand operation ntivibration table Stage-R7625, rotating Slide holder IO I Single-hand operation Microscope Stage-R7650, condenser holder. rotating for 2 specimens Slide holder IO II Oil immersion for 2 specimens Stage-RL7625, with stage bracket + rotating for 1 specimen Slide holder IO I left DM 5000 DM 5500 DM 6000 DM6000 only: Slide holder IO I Single-hand operation for 1 specimen for mot. stage Stage mot., with stage bracket and condenser holder Slide holder IO II Oil immersion for 2 specimens for mot. stage 10

11 4 channel in and 4 out dapter peripheral N-ox triggering Regular fixed stage plate Fixed heating stage plate Fixed stage plate Objective guide for special holder frame Special holder frames Stage Inserts Fast FIM FFIM onnection cable Fura Fura2-set FP/YFP FRET onnection cable Please use filter cube set for FP/YFP FRET Scanning Stage H117 N1 DM (cannot be combined with Incubator L) Regular 3-plate stage Plate stage motorized (TR 127x83) Universal mounting frame Pecon inserts and O2 overs Stage insert for microtiter plates O2 cover for stage insert microtiter plates Triple Triple-band-set hutter cable uide onnection cable Scanning stage motors below stage (TR127x83) dapter cable Insert N O2 cover for temp. insert P ell hamber PO-R cell cultivation system Perfusion insert, open with cover for PO-R ica SFL7000 (not available in the US) Water Immersion Micro Dispenser (DMI6000 only) limate hambers Inverted Incubator L / 230V / 230V / 115V / 115V Heating control unit Temp control unit DMI 4000 DMI Incubator L TIRF O2 ontroller including Humidifier F including F6000 core licence, P and TR6500 HS Upgrade Kit F6500 to F / 230V / 115V O2 controller with accessories 11

12 Shutter Shutter Itensity Itensity Shuter Power Shuter Power lass 1 Laser System Overview Leica F Keyboard US Keyboard UK Keyboard Italian Keyboard German Keyboard Spanish Keyboard Swedish Keyboard French ameras x -mount adapter x -mount adapter Leica DF360 FX Trigger cable DF ameras for TR Leica DF345 FX Trigger cable DF ameras for TR Leica DF310 FX Trigger cable DF ameras for TR oolsnap HQ2 PI board version Trigger cable Roper cameras for TR ascade II Trigger cable Roper cameras for TR Hamamatsu EM-D high speed set Trigger cable Hamamatsu cameras for TR Hamamatsu EM-D back illuminated Trigger cable Hamamatsu cameras for TR Hamamatsu Orca R 2 D Trigger cable Hamamatsu Orca R 2 for TR7000 Peripheral triggering 2 channel in and 1 out dapter peripheral triggering ndor ixon DU-897 EM D back illuminated Trigger cable ndor cameras for TR7000 3h 2x Monitor 19 2x Monitor 22 limate hamber Upright ndor ixon DU-885 EM-D high speed set Trigger cable ndor cameras for TR High Speed shutter cable Liquid light guide dapter 1" Incubator for DM Leica External FW / 230V / 115V Heating control unit / 230V / 115V Temp control unit Digital / 230V / 115V Temp control unit nalog Software Options Live Data Mode D Visualization olocalization D Deconvolution D Deconvolution Dye Finder FRET Software ore Review Software w/o Deconvolution Well Plate cquisition (DMI6000 only, requires motorized/scanning stage) 3h dapter 1" Holder for two FW sliders SFL High Speed Shutter ca Liquid light guide Stage-S mot condenser holder Holder for glass slides 3"x1" Leica SFL7 (not availa Stage-E7650, rotating Slide holder ERGO Single-hand operation System Desk TIRF TIRF M Retrofit Kit Obj. HX PL PO 100x/1.47 Oil Obj. HX PL PO 63x/1.47 Oil Stage-R7625, rotating Slide holder IO I Single-hand operation ntivibration table Stage-R7650, condenser holder. rotating for 2 specimens Slide holder IO II Oil immersion for 2 specimens Microscope Stage-RL7625, with stage bracket + rotating for 1 specimen Slide holder IO I left Fast Z-Movement DM6000 only: Slide holder IO I Single-hand operation for 1 specimen for mot. stage DM 5000 DM 5500 DM SuperZ Galvo Focus F7000 for mot. stage Stage mot., with stage bracket and condenser holder Slide holder IO II Oil immersion for 2 specimens for mot. stage 12

13 4 channel in and 4 out dapter peripheral N-ox triggering Regular fixed stage plate Fixed heating stage plate Fixed stage plate Objective guide for special holder frame Special holder frames Stage Inserts Fast FIM FFIM onnection cable Fura Fura2-set onnection cable Scanning Stage H117 N1 DM (cannot be combined with Incubator L) Regular 3-plate stage Universal mounting frame Pecon inserts and O2 overs Stage insert for microtiter plates O2 cover for stage insert microtiter plates ell hamber PO-R cell cultivation system Perfusion insert, open with cover for PO-R SuperZ Galvo Inserts ble 000 ble in the US) FP/YFP FRET FRET FP/YFP set 2x x onnection cable mount 0.7x for EFW Filter system FP Filter system YFP Triple Triple-band-set onnection cable TIRF Lasers M ox w/o 405nm M ox incl. 405nm r lon laser kit Stages Plate stage motorized (TR 127x83) Scanning stage motors below stage (TR 127x83) dapter cable Insert N O2 cover for temp. insert P PIFO kit F7000 Fast Z-Movement SuperZ Galvo Focus F7000 for 3-plate stage Insert for SuperZ rotable Insert for SuperZ fix (comes with SuperZ Galvo) Insert for SuperZ universal Insert SuperZ for Microplates Insert SuperZ for MatTek dish 35 mm Insert SuperZ for Willco dish 35 mm Insert SuperZ for Willco and MatTek dish 50 mm Incubator SuperZ for petri dishes Incubator SuperZ for Microplates Water Immersion Micro Dispenser (DMI6000 only) limate hambers Inverted Incubator L / 230V / 230V / 115V / 115V Heating control unit Temp control unit F including F6000 core licence, P and TR7000 HS DMI 4000 DMI Incubator L TIRF O2 ontroller including Humidifier / 230V / 115V O2 controller with accessories 13

14 Installation Requirements Environment Room temperature: +18 to + 25, low fluctuation of temperature void proximity to air conditioning equipment Protect from dust Room darkening recommended ir conditioned room recommended Humidity max 80% (35 ) Electricity supply* 115 / 230 V / +/ 10%, 60/50 Hz Two separate fused power lines: 1 x 1600 V for main system 1 x 700 V for climate controller and monitors Heat load* System: approx W limate controller: approx. 300 W (stand-by) rating (width, depth and height in mm)* maximum box size: (1470/1000/1030), max weight: 236 kg (if optional work desk and/or antivibration table is included) Pallets for other hardware components * maximum numbers for the Leica dvanced Widefield System line Leica F6000 with Leica M205 F stereomicroscope and Leica EL6000 external light source 14

15 1405 mm 730mm Distance from wall 400 mm Distance from wall 400 mm 780 mm 2000 mm Maximum footprint of the Leica dvanced Widefield System line with antivibration table and system desk. Leica F7000 with Leica DMI6000 inverted microscope, climate chamber, O 2 controller and SuperZ Galvo focus 15

16 Leica Microsystems the brand for outstanding products Leica Microsystems operates internationally in four divisions, where we rank with the market leaders. Life Science Research Division Leica Microsystems Life Science Research Division supports the imaging needs of the scientific community with advanced innovation and technical expertise for the visualization, measurement and analysis of microstructures. Our strong focus on understanding scientific applications puts Leica Microsystems customers at the leading edge of science. Industry Division The Leica Microsystems Industry Division s focus is to support customers pursuit of the highest quality end result by providing the best and most innovative imaging systems for their needs to see, measure and analyze the microstructures in routine and research industrial applications, in materials science and quality control, in forensic science investigations, and educational applications. iosystems Division The iosystems Division of Leica Microsystems brings histopathology labs and researchers the highest-quality, most comprehensive product range. From patient to pathologist, the range includes the ideal product for each histology step and high-productivity workflow solutions for the entire lab. With complete histology systems featuring innovative automation and Novocastra reagents, the iosystems Division creates better patient care through rapid turnaround, diagnostic confidence and close customer collaboration. Surgical Division The Leica Microsystems Surgical Division s focus is to partner with and support micro-surgeons and their care of patients with the highest-quality, most innovative surgical microscope technology today and into the future. Leica Microsystems mission is to be the world s first-choice provider of innovative solutions to our customers needs for vision, measurement and analysis of micro-structures. Leica, the leading brand for microscopes and scientific instruments, developed from five brand names, all with a long tradition: Wild, Leitz, Reichert, Jung and ambridge Instruments. Yet Leica symbolizes innovation as well as tradition. Leica Microsystems an international company with a strong network of customer services ustralia: North Ryde Tel Fax ustria: Vienna Tel Fax elgium: Groot ijgaarden Tel Fax anada: Richmond Hill/Ontario Tel Fax Denmark: Herlev Tel Fax France: Rueil-Malmaison Tel Fax Germany: Wetzlar Tel Fax Italy: Milan Tel Fax Japan: Tokyo Tel Fax Korea: Seoul Tel Fax Netherlands: Rijswijk Tel Fax People s Rep. of hina: Hong Kong Tel Fax Portugal: Lisbon Tel Fax Singapore Tel Fax Spain: arcelona Tel Fax Sweden: Kista Tel Fax Switzerland: Heerbrugg Tel Fax United Kingdom: Milton Keynes Tel Fax US: annockburn/lllinois Tel Fax and representatives of Leica Microsystems in more than 100 countries. June 2009 LEI and the Leica Logo are registered trademarks of Leica IR GmbH.