Leica SR GSD. Technical Data May 2013

Transcription

1 Leica SR GSD Technical Data May 2013

2 Redefine the Limits of Microscopy Widefield super-resolution with ground state depletion Maximum resolution down to 20 nm The SuMo Stage, with Suppressed Motion technology, minimizes drift for accurate localization of molecules Online super-resolution image projection see results as they are acquired Full application flexibility offered by combining super-resolution with TIRF and epifluorescence on a multi-purpose live cell imaging system Standard fluorochromes can be used no need to change protocols Powerful lasers for the highest flexibility in fluorochrome selection Large set of powerful image processing tools 2

3 Technical Specifications Hardware Microscope stand Leica DMI6000 B / M TIRF MC Right sideport obligatory: used for camera ndor ixon3 897 Left sideport optional for additional camera Bottom port not supported Sample stage SuMo Stage Special stage for GSD super-resolution Different stages for standard applications can be exchanged by customer Objective (GSD) Objectives (additional) Lasers: Wavelength / Power HCX PL PO 100x/1.47 Oil CORR TIRF PIFOC 405 nm / 30 mw 488 nm / 300 mw 532 nm / 500 mw 642 nm / 500 mw Mounted at SuMo stage Needed for GSD super-resolution imaging DIC prism pairs for GSD systems Mounted to nosepiece with 7.5 mm adapter ring TIRF + EPI-Fluorescence: ll lasers for fluorescence imaging, regulated down to max. 50mW GSD super-resolution: 488, 532, 642 nm for widefield/tirf 405 nm for backpumping (control of single molecule switching behavior) Condenser Condenser head 0.55 S28 ( ) Mandatory for any GSD system Condenser S1/S28, mot./fix ( ) Filter cubes , , Special filter cubes available for GSD imaging General Specifications Contrast methods GSD super-resolution, TIRF, EPI-Fluorescence, Bright Field, DIC TIRF functionality Fully integrated Fully automated calibration System can be used in standard TIRF and EPI mode (standard LS F GUI environment) GSD super-resolution: TIRF and EPI mode available, fixed ROI 180 x 180 µm Software LS F Release or higher Standard TIRF functionality supported GSD implemented in integrated software wizard GSD Super-resolution Lateral resolution <50 nm Proper sample preparation obligatory Relevant for performance qualification / warranty Reachable under ideal imaging conditions Down to 25 nm Camera ndor ixon3 897 EM-CCD, necessary for GSD imaging Objective lens HCX PL PO 100x/1.47 Special TIRF lens for GSD super-resolution, Oil CORR TIRF PIFOC Field of view with GSD objective SR image processing Supported Dyes 18 x 18 μm: 51 x 51 μm: 90 x 67 μm: Up to 250 μm: Real-time, various post-processing options lexa 488, tto 488, Rhodamine-6G, tto 532, lexa 532, lexa 546, tto 565, lexa 568, lexa 647, YFP, etc.. GSD: ndor camera (Sub-ROI), 1.6x MagChanger TIRF + GSD preview: ndor camera, 1.6x MagChanger DFC365 FX, 1x C-Mount (optional for standard TIRF/fluo. Imaging) Supported visual field, depending on camera + optics configuration 3

4 Laser rack Inverted research microscope Leica DMI6000 B ctive anti vibration table nti vibration table Leica EL6000 external light source Leica CTR7000 control box Computer workstation Piezo controller TIRF/GSD Module dditional objectives Order number HC PL PO 10x/ HCX PL FLUOTR L 20x/0.40 CORR HC PLN PO 20x/ HCX PL FL L 40x/0.60 CORR HCX PL PO 40x/ OIL HCX PL FLUOTR L 63x/0.70 CORR HCX PL FLUOTR 63x/0.90 CORR HCX PL PO CS 63x/1.20 W CORR HCX PL PO 63x/1.30 GLYC 37 C HCX PL PO CS 63x/1.30 GLYC 37 C HCX PL PO CS 100x/ OIL HCX PL PO CS 100/1.46 OIL HCX PL PO 100/1.47 OIL

5 1405 mm 750mm Distance from wall 600 mm 2600 mm Distance from wall 600 mm 1500 mm Maximum footprint of the Leica SR GSD with antivibration table, laser rack and system desk. Installation Requirements Environment Room temperature: +15 to + 30 C ir condition required, but avoid proximity to GSD system Protect from dust Room darkening recommended Humidity max 80%, non condensing Temperature stability ±1 C (±1.8 F) ; ΔT<0.5 C/15min Electricity supply The system requires two mutually independent fuse circuits. One fuse circuit for the main system and the second for the laser rack. Heat load Crating (width, depth and height in mm)* 2x Supply voltage 115 VC ± 10%: 15 mpere 2x Supply voltage 230 VC ± 10%: 12 mpere System: approx. 2 kw Climate controller: approx. 300 W Cooling capacity: max. 2.5 kw waste heat Pallets: 2x 1200/800/1400, 1x 750/750/1100 max weight: 450 kg (if optional work desk and/or antivibration table is included) Pallets for other hardware components * maximum numbers for the Leica SR GSD Systems 5

6 Shutter Shutter Itensity Itensity Shuter Power Shuter Power Leica SR GSD Keyboard US Keyboard UK Keyboard Italian Keyboard German Keyboard Spanish Cameras x C-mount adapter ndor ixon DU-897 EM CCD back illuminated Trigger cable ndor cameras Leica DFC365 FX Trigger cable DFC Cameras Leica DFC345 FX Trigger cable DFC Cameras * Peripheral triggering 2 channel in and 1 out dapter peripheral triggering Keyboard Swedish Keyboard French x C-mount adapter Leica DFC310 FX Trigger cable DFC Cameras Hamamatsu EM-CCD high speed set Trigger cable Hamamatsu cameras Hamamatsu EM-CCD back illuminated Trigger cable Hamamatsu cameras Hamamatsu Orca R 2 CCD Trigger cable Hamamatsu Orca R Photometrics Evolve Camera EM CCD High speed trigger cable for Evolve cameras EL x on request Monitor 30 2x Monitor 22 Software Options Leica Workstation GSD HP Z800 cquisition Modules F6000 Core Dongle for F6000 Extensions F6000 Multi-Channel cquisition F6000 Time-Lapse F6000 Z-Control and Software utofocus** F6000 Mark and Find* F6000 Stitching* F6000 Camera Driver Non-Leica Cameras Packages F6000 Premium incl. PC (F6000 Core, Dongle, Multi-Channel, Time-Lapse, Z-Control, Mark & Find, Stitching, Calcium Imaging, 3D-Visualization) F6000 Expert incl. PC (F6000 Core, Dongle, Multi-Channel, Time-Lapse, Z-Control, 3D-Visualization) dvanced Modules Calcium Imaging (Requires Multi-Channel cquisition, Time-Lapse) Live Data Mode D Visualization Colocalization D Deconvolution D Deconvolution Dye Finder FRET Software (Requires Multi-Channel cquisition, Time-Lapse) * Only available for configurations with motorized stage ** Requires motorized z-drive * Default camera - always needed for GSD System Desk ccurion nti Vibration Table 3h EL High Speed shutter cable Liquid light guide dapter 1" EL dapter 1" EL Leica External FW 3h High Speed Shu Liquid light guid Holder for two FW sliders High-Speed Mic Boxes High-Speed Mic Box Core High-Speed Board Extension Board for 2 Filter Wheels High-Speed Board Extension Board 7000 (for 4 Filter Wheels) Climate Chambers Inv Incubator BLX Incubator BLX Black C Incubator BLX TIRF Leica DMI6000 B 6

7 Optional stages for system used as imaging platform (exchange of GSD stage with following stages possible) 4 channel in and 4 out dapter peripheral BNC-Box triggering Regular fixed stage plate Fixed heating stage plate Fixed cooling stage plate Objective guide for special holder frame Special holder frames Stage Inserts tter cable e erted Fast FIM FFIM Filterwheel slider short Connection cable Fura Fura2-set Filterwheel slider short Connection cable CFP/YFP FRET FRET CFP/YFP set 2x Filterwheel slider short 2x Connection cable C-mount 0.7x for EFW Filter system CFP Filter system YFP Triple Triple-band-set Filterwheel slider short Connection cable / 230V / 230V / 115V / 115V Heating control unit Temp control unit CO2 Controller including Humidifier C Scanning Stage H117 N1 DM (requires High-Speed Board 6500, not compatible with Water Immersion Dispenser) Regular 3-plate stage Plate stage motorized (TR 127x83) Scanning stage motors below stage TR 127x83 (requires High-Speed Board 6500) dapter cable SCN plus IM 130x85 Scanning stage with Encoder incl. Controller (not available for Matrix M3 and Well Plate cquisition modules) Universal mounting frame Pecon inserts and CO2 Covers Insert N Fast Z-Movement CO2 cover for stage inse microtiter platesrt CO2 cover for temp. insert P SuperZ Galvo Focus F7000 for 3-plate stage (requires Extension Board 7000) Insert for SuperZ rotable Insert for SuperZ fix (comes with SuperZ Galvo) Cell Chamber Stage insert for microtiter plates POC-R cell cultivation system Perfusion insert, open with cover for POC-R SuperZ Galvo Inserts Insert SuperZ for MatTek dish 35 mm Insert SuperZ for Willco dish 35 mm Insert for SuperZ universal Insert GL-Set including holders for IBIDI and LabTec chambers Insert SuperZ for Microplates Insert SuperZ for Willco and MatTek dish 50 mm Incubator SuperZ for petri dishes CO2 cover for Insert GL Incubator SuperZ for Microplates / 230V / 115V CO2 controller with accessories GSD Module (requires Extension Board 7000, for DMI6000 B only) GSD system core incl. ctive Vibrationisolation table Leica SuMo sample stage OPTIK Kit GSD GSD SW Module GSD Lasers Laser rack GSD laser MPBC Visible Laser 532nm MPBC Visible Laser 488nm * MPBC Visible Laser 642nm * *optional lasers C 7

8 The statement by Ernst Leitz in 1907, With the User, For the User, describes the fruitful collaboration with end users and driving force of innovation at Leica Microsystems. We have developed five brand values to live up to this tradition: Pioneering, High-end Quality, Team Spirit, Dedication to Science, and Continuous Improvement. For us, living up to these values means: Living up to Life. Leica Microsystems operates globally in four divisions, where we rank with the market leaders. Life Science Division The Leica Microsystems Life Science Division supports the imaging needs of the scientific community with advanced innovation and technical expertise for the visualization, measurement, and analysis of microstructures. Our strong focus on understanding scientific applications puts Leica Microsystems customers at the leading edge of science. Industry Division The Leica Microsystems Industry Division s focus is to support customers pursuit of the highest quality end result. Leica Microsystems provide the best and most innovative imaging systems to see, measure, and analyze the microstructures in routine and research industrial applications, materials science, quality control, forensic science investigation, and educational applications. Medical Division The Leica Microsystems Medical Division s focus is to partner with and support surgeons and their care of patients with the highest-quality, most innovative surgical microscope technology today and into the future. Leica Microsystems an international company with a strong network of worldwide customer services: ctive worldwide Tel. Fax ustralia North Ryde ustria Vienna Belgium Groot Bijgaarden Canada Concord/Ontario Denmark Ballerup France Nanterre Cedex Germany Wetzlar Italy Milan Japan Tokyo Korea Seoul Netherlands Rijswijk People s Rep. of China Hong Kong Shanghai Portugal Lisbon Singapore Spain Barcelona Sweden Kista Switzerland Heerbrugg United Kingdom Milton Keynes US Buffalo Grove/lllinois May 2013 Copyright by Leica Microsystems CMS GmbH, Wetzlar, Germany, Subject to modifications. LEIC and the Leica Logo are registered trademarks of Leica Microsystems IR GmbH.