AZURA instruments & components for (U)HPLC, Prep LC, FPLC and osmometry

Transcription

1 Product Selection Guide 2017/2018 AZURA instruments & components for (U)HPLC, Prep LC, FPLC and osmometry Made in Germany KNAUER Wissenschaftliche Geräte GmbH Hegauer Weg Berlin

2 Science Together Welcome to KNAUER - manufacturer of liquid chromatography instruments and solutions! KNAUER ("KNAUER Wissenschaftliche Geräte GmbH") is an owner-managed middle-sized company situated in Berlin, Germany. Since 1962, we have been developing, manufacturing and distributing laboratory instruments around the world. With more than 120 employees we are the oldest German manufacturer of instruments for HPLC, FPLC, simulated moving bed chromatography (SMB) and osmometry. Our product portfolio ranges from most compact analytical HPLC systems to SMB systems for the extraction of up to kg of pure substance per year. Products and services offered include state of the art HPLC/ UHPLC systems, FPLC systems, solutions for the purification of value products and fine chemicals, LC columns, and method development. Alexandra Knauer (CEO and owner) The source of our success are numerous world s firsts that have won more than 20 awards for innovation. We strive to grow continuously, to expand our expertises and to discover new market opportunities. Quality and environmental management The KNAUER quality management system according to DIN EN ISO 9001 makes sure that we continuously produce products in the best quality possible. As a company, we have to prove ourselves every day in a highly competitive market. Therefore, we can only be successful if every employee contributes his share. Your satisfaction with the quality of our products and services is the key to our success. In addition to our quality management system, KNAUER contributes to the conservation of a healthy environment by basing our work on an environmental management system according to DIN EN ISO Starting with the development of a new product, our designing engineers are bound to use long-lasting materials which allow low maintenance time intervals and minimal downtime. Furthermore, a high energy efficiency and material efficiency in the production process and during the use of our products are important to us. Finally, the products should be able to be disposed of in an environmentally compatible way. Every single part and original spare part corresponds to our strict criteria for construction, material and safety. This is guaranteed by our quality assurance system. And if your products need replacement or repair, it will be as good as a new with original spare parts from KNAUER. The quality management system and the working procedures that come with it are being updated on a regular basis. All KNAUER KNAUER among Top 100 innovators. employees know the procedures of the quality management system and its implementation. Continuous improvement is one of our goals and overall basic management principles. Your remarks and suggestions are a substantial part of our daily work. They help us become even more precise, faster and more customer friendly. Please let us know what your suggestions are and what we can do better. Please do not hesitate to contact our quality management team at quality@knauer.net!

3 Table of contents (U)HPLC pump AZURA Pump P 6.1L 1 AZURA Pump P 6.1L with 5 ml pump head 2 AZURA Pump P 6.1L with 10 ml pump head 2 AZURA Pump P 6.1L semi-prep 3 AZURA Pump P 6.1L metal-free 3 Preparative HPLC pump AZURA Pump P 2.1L 4 Compact HPLC pumps AZURA Pump P 4.1S 5 AZURA Pump P 2.1S 6 Assistant AZURA Assistant ASM 2.1L 7 Autosampler AZURA Autosampler AS 6.1L 9 Column thermostat AZURA Column Thermostat CT Diode array detectors AZURA Detector DAD 6.1L 12 AZURA Detector DAD 2.1L & MWD 2.1L 13 UV/VIS detectors AZURA Detector UVD 2.1L 14 BlueShadow UV/VIS Detector 50D 15 AZURA Detector UVD 2.1S 16 Refractive index detector AZURA Detector RID 2.1L 17 Fluorescence detector Fluorescence Detector RF-20/Axs 18 Light scattering detector Light Scattering Detector Sedex LC/85LT/90LT 19 Electrochemical detector Electrochemical Detector DECADE Elite SCC 20 Conductivity detector Conductivity detector CDD-10 Avp 21 Conductivity/pH monitor AZURA Conductivity Monitor CM 2.1S 22 Fraction collectors Foxy R1 and Foxy R2 23 LABOCOL Vario Electrical valves AZURA Valve Drive V 2.1S Multiposition 26 Electric valves for many injection and/or switching tasks 27 Manual valves Manual injection and multi-position switching valves 28

4 Table of contents Ultra high-pressure valves 2-position and multi-position valve 29 Online degasser AZURA Degasser DG 2.1S 30 Osmometer K-7400S Semi-Micro Osmometer 31 Software Mobile Control (Chrom) for Windows ClarityChrom 33 PurityChrom 34 OpenLAB CDS EZChrom Edition 35 Thermo Scientific Dionex Chromeleon chromatography data system driver 36 PC hardware & IT accessories / power connections PC German 37 PC international 37 PC special edition 37 Network devices 37 IT accessories 37 Power connections 37 Column kits RP column kit 38 NP column kit 38 Mixed column kit 38 GPC column kit 38 SEC column kit 38 Pump accessories Eluent tray and bottles 39 Solvent filters and pump inlet tubing 39 Static mixers 39 Dynamic mixers 39 Pump head inlet fittings 40 Pump head outlet fittings 40 Mass flow controllers 40 Replacement pump head AZURA Pump P 4.1S & P 2.1S 41 Replacement pump head AZURA Pump P 6.1L 41 Replacement pump head BlueShadow 40P 41 Replacement pump head AZURA Pump P 2.1L / Blue Shadow 80P 41 Check valves 41 Maintenance kits 42 Autosampler accessories AZURA Autosampler AS 6.1L 42 Detector accessories Flow cells 1/16 43 Flow cells 1/8 43 Flow cells 1/4 43 Flow cells 1/16 fiber optics 43 Flow cells 1/8 fiber optics 43 Nano flow cell 43 Flow cells >1/8 fiber optics 44 Fiber optic cables 44 Waste tubing kits 44 Lamps 44 1

5 Table of contents Valve accessories Valve head 1/16 stainless steel 45 Valve head 1/8 stainless steel 45 Valve head 1/16 PEEK 45 Valve head 1/8 PEEK 45 Rotor seals 45 Sample loop stainless steel, 1/16 46 Sample loop stainless steel, 1/8 46 Sample loop PEEK, 1/16 46 Sample loop PEEK, 1/8" 46 Injection syringes 47 Luer-Lock glass syringes 47 Loop filling ports 47 AZURA mounting brackets 47 Services / Qualification Installation Qualification (IQ) - scope of delivery with all AZURA instruments 48 Installation Qualification (IQ) - software 48 Operation Qualification (OQ) - pumps 48 Operation Qualification (OQ) - autosampler 48 Operation Qualification (OQ) - detectors 48 Operation Qualification (OQ) - flow cells 48 Operation Qualification (OQ) - osmometer 48 Operation Qualification (OQ) - valves 48 Operation Qualification (OQ) - fraction collectors 48 Operation Qualification (OQ) - column thermostat 48 Performance Verification (PV) 48 Accessories Fittings K-Connect system - Kits consisting of bushings, clamping and sealing rings 49 DYNASEAL system - Kits consisting of bushings, clamping and sealing rings 49 Bushings for capillaries - DYNASEAL 49 Split-grooved clamping rings 49 Sealing rings 49 Biconical sealing rings 49 Bushings for capillaries, 1/16, stainless steel 50 Bushings for capillaries, 1/8, stainless steel 50 Ferrules for capillaries 50 Bushings for capillaries, 1/16"/1/8", PEEK & polymer 50 Flat bottom fittings and adapters 50 Accessories Caps/connectors Caps (blind fittings) 51 Low dead volume unions, polymer/peek 51 Low dead volume unions, stainless steel /titanium 51 Unions and reducing unions, stainless steel 51 T-connectors, stainless steel/titanium 52 T-connectors, polymer 52 X-connectors, stainless steel 52 X-connectors, polymer 52 Pressure release valves 52 Backpressure regulators 52 Accessories - Capillaries Capillaries, stainless steel, 1/16" 53 Capillaries, titanium, 1/16" 53 Capillaries, stainless steel, 1/8" 53 AZURA Analytical K-Connect Start-up Kits, stainless steel, 1/32" capillaries with fitting sleeves for 1/16"connections: Set of precut capillaries, connections, adapters and low dead volume couplings 53 2

6 Table of contents Accessories - Capillaries Capillaries, AZURA Analytical K-Connect, stainless steel, 1/32" with fitting sleeve for 1/16"connections 53 AZURA Capillary start-up kit, stainless steel 53 Accessories - Tubings Tubing start-up kits for FPLC 54 PEEK tubings, 1/16" 54 PEEK tubings, 1/8" 54 Tefzel tubings, 1/16" 54 Tefzel tubings, 1/8" 54 PTFE tubings, various OD 54 Accessories - Inline filters / osmometry Inline filters, stainless steel, for HPLC 55 Inline filters, biocompatible, for FPLC 55 Shut-off valves 55 Adapters 55 Osmometry accessories 55 Accessories - Eluent and column heating heating / purification High flow eluent and column heating devices 56 Purification 56 Accessories - Vials / caps kits Vials and caps kits for analytical HPLC 57 Vials and caps kits including microinserts for analytical HPLC 57 Vials and caps kits for preparative HPLC 57 Pliers for analytical and preparative vials 57 Sample tubes for osmometry 57 Accessories - Tools Wrenches and tightening tools 58 Capillary and tube cutter 58 Capillary grater and bender 58 Tool sets 58 Accessories - Racks / column holder LC racks 59 LC column holder 59 Installation accessories 59 Application & Academy 60 Supported devices Pumps 61 Detectors 62 Assistant 65 Autosamplers 65 Analog/digital converters 66 Thermostats/column ovens 67 Electrical valve drives 67 Other supported devices 67 Fraction collectors 68 Mobile Control 69 Other software 70 Conversion tables 71 Notes 72 Order request 73 3

7 (U)HPLC pump AZURA Pump P 6.1L The AZURA Pump P 6.1L uses technology to overcome the challenges of pumping LC solvents at high pressure and high flow rates. This pump is designed to fulfill the needs for high pressure and low pressure mixing tasks. The pump can deliver flow in the range of ml/min at pressures up to 1000 bar (depending on model). The AZURA binary pump contains two identical high pressure pumps, a 2 2-channel inlet solvent selection valve and the new developed AZURA mixer, a low-volume mixing device. The AZURA quaternary pump contains one high pressure pump and an integrated LPG mixing block with a 4 channel inlet solvent selection valve and the new developed AZURA mixer, a lowvolume mixing device. The integrated degasser and AZURA inline filter are completing the analytical AZURA HPLC pump and turn this pump into a working horse in the lab. For biocompatible applications or ion chromatography this pump is also available with a complete metal free design. A variety of software control options is available: KNAUER offers various options for drivers. Detailed information on page 61. General specifications Solvent delivery Pulsation compensation active pulsation compensation Solvent selection valve 2 x 2 channels (HPG only) Flow rate accuracy < 1%, measured at 5-50% of flow range using ethanol/water 10:90 Flow rate precision < 0.1% RSD Gradient formation High pressure binary mixing Gradient range 0-100% HPG: minimum increment 0.1% HPG: gradient accuracy ± 1% (5-95%, measured at 5-50% of flow range, water/ caffeine tracer) HPG: gradient precision 1% RSD, based on retention time at constant room temperature Piston seal washing standard System protection soft start, P min and P max are programmable Wetted materials stainless steel, graphite fiber reinforced PTFE, FKM, PEEK, sapphire, aluminium oxide (Al2O3) Degasser module (optional) Degasser channels 4 channels, Teflon AF (LPG module), 2 channels Teflon AF (HPG modules) Degasser maximum flow rate 10 ml/min Degassing method gas permeation through Teflon AF amorphous fluoropolymer membrane Degassing efficiency < 0.5 ppm dissolved O2 at 1 ml/min Degassing chamber volume 480 µl volume per channel Solvent applicability universal, with the exception of hydrochloric acid and halogenated hydrocarbons - in particular hexafluor Wetted materials PEEK, Tefzel, Teflon AF Vacuum chamber polypropylene and stainless steel Vacuum pump low hysteresis behavior Communication Control LAN; analog and event controlled Display Mobile Control (optional) Analog inputs 0-10 V Analog control input flow rate Technical parameters Ambient conditions temperature range: C; F air humidity: below 90% (non condensing) Leak sensor yes General Power supply voltage range: V, Hz Dimensions 361 x x 523 mm (W x H x D) 1

8 (U)HPLC pump AZURA Pump P 6.1L with 5 ml pump head Specifications Pump head Pump head materials Maximum delivery pressure Flow rate range Flow rate increment 5 ml/min stainless steel 100 Mpa (1000 bar, psi) up to 2 ml/min, 70 MPa (700 bar, psi) ml/min ml/min Ordering details: APH34GA APH35GA AZURA Pump P 6.1L (LPG), with 5 ml pump head (stainless steel), degasser and mixer (100 µl) AZURA Pump P 6.1L (HPG), with 5 ml pump head (stainless steel), degasser and mixer (100 µl) AZURA Pump P 6.1L with 10 ml pump head Specifications Pump head Pump head materials Maximum delivery pressure Flow rate range Flow rate increment 10 ml/min stainless steel 70 Mpa (700 bar, psi) up to 5 ml/min, 40 MPa (400 bar, 5800 psi) ml/min ml/min Ordering details: APH30EA APH30ED APH34EA APH35EA APH35ED APH38EA APH38ED APH39EA AZURA Pump P 6.1L isocratic, without degasser, with 10 ml pump head (stainless steel) AZURA Pump P 6.1L isocratic, without degasser, with 10 ml NP pump head (stainless steel) AZURA Pump P 6.1L (LPG), with 10 ml pump head (stainless steel), degasser and mixer (100 µl) AZURA Pump P 6.1L (HPG), with 10 ml pump head (stainless steel), degasser and mixer (100 µl) AZURA Pump P 6.1L (HPG), with 10 ml NP pump head (stainless steel), degasser and mixer (100 µl) AZURA Pump P 6.1L (HPG), without degasser, with 10 ml pump head AZURA Pump P 6.1L (HPG), without degasser, with 10 ml NP pump head and mixer (100 µl) AZURA Pump P 6.1L (LPG), without degasser, with 10 ml pump head (stainless steel) and mixer (100 µl) 2

9 (U)HPLC pump AZURA Pump P 6.1L semi-prep Specifications Pump head Pump head materials Maximum delivery pressure Flow rate range Flow rate increment 50 ml/min stainless steel 30 Mpa (300 bar, 4351 psi) up to 10 ml/min, 20 MPa (200 bar, 2900 psi) ml/min ml/min Ordering details: APH30FA APH38FA AZURA Pump P 6.1L isocratic, without degasser, with 50 ml pump head (stainless steel) AZURA Pump P 6.1L (HPG), without degasser, with 50 ml pump head (stainless steel) and mixer (200 µl) AZURA Pump P 6.1L metal-free Specifications Pump head Pump head materials Maximum delivery pressure Flow rate range Flow rate increment 10 ml/min or 50 ml/min ceramic 10 ml pump head: 40 MPa (400 bar, 5800 psi) 50 ml pump head: 20 MPa (200 bar, 2900 psi) ml/min or ml/min ml/min Ordering details: APH60EB APH60FB APH64EB APH65EB APH68EB APH68FB APH69EB AZURA Pump P 6.1L, isocratic, without degasser, with 10 ml pump head (ceramic) AZURA Pump P 6.1L, isocratic, without degasser, with 50 ml pump head (ceramic) AZURA Pump P 6.1L (LPG), with 10 ml pump head (ceramic), degasser and mixer (250 µl) AZURA Pump P 6.1L (HPG), with 10 ml pump head (ceramic), degasser and mixer (250 µl) AZURA Pump P 6.1L (HPG), without degasser, with 10 ml pump head (ceramic) and mixer (250 µl) AZURA Pump P 6.1L (HPG), without degasser, with 50 ml pump head (ceramic) and mixer (250 µl) AZURA Pump P 6.1L (LPG), without degasser, with 10 ml pump head (ceramic) and mixer (250 µl) 3

10 Preparative HPLC pump AZURA P 2.1L Preparative HPLC pump AZURA preparative HPLC pump P 2.1L covers a wide flow rate range and pressure capabilities. It has been designed for purification of mg and gram samples. The integrated automatic recognition of the pump head with RFID technology allows fast adaptions of the pump for various applications. A variety of software control options is available: KNAUER offers various options for drivers. Detailed information on page 61. Specifications Solvent delivery Pump type preparative HPLC pump Delivery system double piston pump with Al2O3 pistons Pump head 100 ml / 250 ml / 500 ml / 1000 ml Pump head materials stainless steel /titanium Maximum delivery pressure for 100 ml pump head: 40 MPa (400 bar, 5800 psi); for 250 ml pump head: 22,5 MPa (225 bar, 3260 psi) up to 100 ml/min, 20 MPa (200 bar, 2900 psi) up to 250 ml/min; for 500 ml pump head: 10 MPa (100 bar, 1450 psi); for 1000 ml pump head: 7.5 MPa (75 bar, 1080 psi) up to 350 ml/min, 5 MPa (50 bar, 720 psi) up to 1000 ml/min Flow rate range ml/min / ml/min / ml/min / ml/min Flow rate increment 0.01 ml/min (0.1 ml/min for 250/500/1000 ml pump head) Flow rate accuracy ± 2%, measured at 5-50% of flow range using ethanol/water 10:90 Flow rate precision < 0.1% RSD Gradient support isocratic, upgradeable to a binary HPG system with additional pump P 2.1L or to a binary or ternary LPG system HPG: gradient accuracy ± 2% (5-95%, measured at 5-50% of flow range, water/caffeine tracer) HPG: gradient precision 1% RSD, based on retention time at constant room temperature LPG: gradient accuracy ± 3% (5-95%, measured at 5-50% of the flow range, water/caffeine tracer) LPG: gradient precision 2% RSD, based on retention time at constant room System protection soft start, Pmin and Pmax are programmable Wetted materials stainless steel, graphite fiber reinforced PTFE, FKM, PEEK, sapphire, aluminum oxide (Al 2O 3), stainless steel Communication Control Display Analog inputs Analog control input Level/event outputs Programming Special features Technical parameters LAN; analog and event controlled Mobile Control (optional) 0-10 V flow rate 8 event outputs (TTL, OC, relays) 19 programs + 9 links (to programs) + 1 wake up program system pump head is detected automatically using radio frequency identification (RFID) Ambient conditions temperature range: C; F air humidity: below 90% humidity (non condensing) Leak sensor yes General Power supply voltage range: V, Hz Dimensions 361 x x 523 mm (W x H x D) Weight 19 kg Optional accessories ternary low pressure gradient valve block, ml/min binary low pressure gradient valve block, ml/min pump head heating and cooling device Ordering details: APE20KA APE20LA APE20MA APE20NA APE20KB APE20LC APE20MC APE20NB AZZ00AA AZZ00AB AZZ10AB P 2.1L Pump P 2.1L with 100 ml pump head (stainless steel) P 2.1L Pump P 2.1L with 250 ml pump head (stainless steel) P 2.1L Pump P 2.1L with 500 ml pump head (stainless steel) P 2.1L Pump P 2.1L with 1000 ml pump head (stainless steel) P 2.1L Pump P 2.1L with 100 ml pump head (titanium) P 2.1L Pump P 2.1L with 250 ml pump head (titanium) P 2.1L Pump P 2.1L with 500 ml pump head (titanium) P 2.1L Pump P 2.1L with 1000 ml pump head (titanium) LPG module for Pump P 2.1L binary up to 800 ml/min LPG module for Pump P 2.1L ternary up to 220 ml/min LPG module for Pump P 2.1L ternary, PEEK max. flowrate up to 220 ml/min 4

11 Compact HPLC pumps AZURA Pump P 4.1S AZURA Pump P 4.1S was developed for eluent delivery up to 400 bar and for flow rates up to 50 ml/ min in HPLC and other applications where a compact easy-to-integrate pump is required. A variety of software control options is available: KNAUER offers various options for drivers. Detailed information on page 61. Key features integrated pressure sensor versatile due to wide flow rate range ( ml/min) powerful and precise drive, with low pulsation compact dimensions flexible control via Ethernet, RS-232 and analog signals Specifications Pump type ultra-compact high pressure pump Delivery system dual piston pump with one working piston, one auxiliary Pump head 10 / 50 ml/min Pump head materials ceramic/sst Maximum delivery pressure 40MPa 10ml & 15MPa 50ml pump head Maximum flow rate 10 ml pump head: ml/min ( ml/min recommended, ml/min continuous operation) 50 ml pump head: ml/min ( ml/min recommended, ml/min continuous operation) Flow rate increment 0.01 ml/min Flow rate accuracy ± 1%, measured at 5 50% of flow range using ethanol/water 10:90 Flow rate precision 0.5 % RSD, measured at 1/ 5 ml/min using ethanol/water 10:90 System protection P min and P max are programmable Wetted materials graphite fiber reinforced PTFE, FKM, PEEK, sapphire, zirconium oxide (ZrO 2), titanium and pump head material Max. viscosity 100 mpa s (at reduced max. flow) Liquid temperature range C Communication Control LAN, RS-232, analog and event controlled Analog inputs 0 10 V Analog control input flow rate Technical parameters Ambient conditions temperature range: C; F air humidity: below 90 % humidity (non-condensing) General Power supply V power supply inclduded, 24 V DC, 60 W Dimensions 113 x 135 x 225 mm (W x H x D) Ordering details: APG20EA APG20EB APG20EF APG20FA APG20FB AZURA Pump P 4.1S compact HPLC pump with 10 ml/min pump head, stainless steel AZURA Pump P 4.1S compact HPLC pump with 10 ml/min pump head, ceramic AZURA Pump P 4.1S compact HPLC pump with 10 ml/min pump head, ceramic, with Ti-connections AZURA Pump P 4.1S compact HPLC pump with 50 ml/min pump head, stainless steel AZURA Pump P 4.1S compact HPLC pump with 50 ml/min pump head, ceramic 5

12 Compact HPLC pumps AZURA Pump P 2.1S AZURA Pump P 2.1S was developed for eluent delivery up to 400 bar and for flow rates up to 50 ml/ min in HPLC and other applications where a compact easy-to-integrate pump is required. A variety of software control options is available: KNAUER offers various options for drivers. Detailed information on page 61. Key features versatile due to wide flow rate range ( ml/min) powerful and precise drive, with low pulsation compact dimensions flexible control via Ethernet, RS-232 and analog signals Specifications Pump type ultra-compact high pressure pump Delivery system dual piston pump with one working piston, one auxiliary Pump head 10 / 50 ml/min Pump head materials ceramic/sst/hastelloy C Maximum delivery pressure 40MPa 10ml & 15MPa 50ml pump head Maximum flow rate 10 ml pump head: ml/min ( ml/min recommended, ml/min continuous operation) 50 ml pump head: ml/min ( ml/min recommended, ml/min continuous operation) Flow rate increment 0.01 ml/min Flow rate accuracy ± 5%, measured at 5-50% of flow range using ethanol/water 10:90 ± 2% at calibration point (one point calibration), measured at 5-50% of flow range using ethanol/water 10:90 Flow rate precision 0.5 % RSD, measured at 1 / 5 ml/min using ethanol/water 10:90 System protection I min and I max are programmable, (I ~ pressure, pump without pressure sensor) Wetted materials graphite fiber reinforced PTFE, FKM (FFKM for APG90EC and APG90FC), PEEK (PCTFE for APG90EC and APG90FC), sapphire, zirconium oxide (ZrO 2) and pump head material Max. viscosity 100 mpa s (at reduced max. flow) Liquid temperature range C Communication Control LAN, RS-232, analog and event controlled Analog inputs 0 10 V Analog control input flow rate Technical parameters Ambient conditions temperature range: C; F air humidity: below 90 % humidity (non-condensing) General Power supply V power supply inclduded, 24 V DC, 60 W Dimensions 113 x 135 x 225 mm (W x H x D) Detailed information on dosing pumps in the Product Selection Guide: Ordering details: APG90EA APG90EB APG90EC APG90FA APG90FB APG90FC AZURA Pump P 2.1S compact HPLC pump with 10 ml/min pump head, stainless steel AZURA Pump P 2.1S compact HPLC pump with 10 ml/min pump head, ceramic AZURA Pump P 2.1S compact HPLC pump with 10 ml/min pump head, Hastelloy C AZURA Pump P 2.1S compact HPLC pump with 50 ml/min pump head, stainless steel AZURA Pump P 2.1S compact HPLC pump with 50 ml/min pump head, ceramic AZURA Pump P 2.1S compact HPLC pump with 50 ml/min pump head, Hastelloy C 6

13 Assistant AZURA Assistant ASM 2.1L A flexible combination module The AZURA Assistant ASM 2.1L is a compact combination module which can be equipped with up to three device modules. Available for selection are valves, pumps, degassers and a UV detector. Depending on the integrated modules, the assistant fulfills many different tasks like sample and buffer selection, sample injection, column switching or fraction collection. An assistant including a pump, valve and detector features a compact chromatographic system. As a part of a larger system the ASM 2.1L is extremely versatile in analytical, preparative and continuous liquid chromatography. The concept of flexible combination of device modules combines highest functionality with minimal space requirements. For detailed information on assistant configuration, service and software features, please visit the assistant site on the KNAUER website: A variety of software control options is available: KNAUER offers various options for drivers. Detailed information on page 65. Specifications Communication Interface LAN Control Mobile Control, software Inputs Error (IN), Start (IN), both TTL Outputs Event 1-3 (OC, Relay), Error (OUT), +5 V, +24 V Analog output integrator output (detector signal) General Power supply V, Hz, maximum 100 W Dimensions 361 x 158 x 523 mm (W x H x D) Weight about 14 kg (depending on integrated modules) Leak sensor yes Technical parameters Ambient conditions temperature range: 4-40 C, F humidity: below 90 % non-condensing Integrated modules Please consider technical parameters of integrated modules. Maximum number of modules for each assistant and configuration notes 1x degasser An assistant with only degasser is not allowed. 1x detector 2x VICI valve drives AWB02 2x pumps No HPG (high pressure gradient) is supported. An AZURA Pump P 4.1S cannot be placed to the right of a detector AZURA Detector UVD 2.1S. Software functions supported by the ASM 2.1L Assistant Configuration: The ASM 2.1L is supported as complete device. Modules are addressed via the assistant. Configure the AZURA Assistant ASM 2.1L: Pumps, Valves, Degasser, UV detector ClarityChrom OpenLAB Mobile Control version 4 / version 5 Two pumps (independent) yes no no / yes Fraction valve one one no / yes, but time-based Injection module no no yes, but part of a method Single device configuration: The ASM 2.1L is not supported as device. Integrated modules are addressed as separate devices via IP port. ClarityChrom OpenLAB PurityChrom Two pumps (independent) no yes yes Fraction valve no cascading (Multi valve fraction collector) Injection module* yes, but part of a method yes, fully automatic module with trigger for data acquisition one yes, but part of a method *An injection module is a combination of one pump and one 6 port 2 position valve. 7

14 Assistant Assistant modules Please consider that listed article numbers represent standalone devices. Pumps Compact Pump AZURA Pump P 2.1S AZURA Pump P 4.1S Pressure sensor No Yes Yes 50 bar Pump head Article number Volume Material stainless steel APG90EA 10 ml Hastelloy C APG90EC ceramic APG90EB stainless steel APG90FA 50 ml Hastelloy C APG90FC ceramic APG90FB 10 ml stainless steel APG20EA ceramic APG20EB 50 ml stainless steel APG20FA ceramic APG20FB 10 ml ceramic APG17EB 50 ml ceramic APG17FB Valves Ports Position Material Connection Bore size Pressure limit Flow rate Article number* 1/16" 0,6 mm 300 bar 100 ml/min AWA10AA 1/16" 0,3 mm 400 bar 50 ml/min AWA11CA stainless steel 1/16" 0,3 mm 1200 bar 10 ml/min AVC28AC & AWB02* 2 Position 1/8" 1,2 mm 300 bar 500 ml/min AWA10AD 1/16" 0,6 mm 200 bar 50 ml/min AWA10AC PEEK 1/8" 1,2 mm 200 bar 500 ml/min AWA10AE 6 Port 1/16" 0,6 mm 300 bar 100 ml/min AWA10BA 1/16" 0,3 mm 400 bar 50 ml/min AWA11DA stainless steel 1/16" 0,3 mm 1200 bar 10 ml/min AVR28AC & AWB02* Multiposition 1/8" 1,2 mm 300 bar 500 ml/min AWA10BC PEEK 1/16" 0,6 mm 200 bar 50 ml/min AWA10BB 1/8" 1,2 mm 200 bar 500 ml/min AWA10BD 8 Port Multiposition stainless steel 1/16" 0,3 mm 1200 bar 10 ml/min AVR38AC & AWB02* 12 Port Multiposition stainless steel 1/8" 1,2 mm 25 bar 500 ml/min AWA20BG 16 Port Multiposition stainless steel 1/16" 0,6 mm 50 bar 100 ml/min AWA30BH * Products with two article numbers consist of valve and valve drive (AWB02) separately. Detector Type Single wavelength Wavelenth range Fiber Optics Connectors Article number UV variable nm No ADA00 Order flow cells on page 43. Degassers Type Channels Article number analytical 2 AZE02 semi-preparative 2 AZE34 8

15 Autosampler AZURA Autosampler AS 6.1L The AZURA Autosampler AS 6.1L can inject from up to 768 positions when equipped with microtiter plates (either high or low formats) or from up to 108 standard 2 ml sample vials. Sample carryover is significantly minimized thanks to a highly-effective interior and exterior needle wash procedure. This autosampler is also fast and flexible: one complete sample injection cycle takes less than one minute, including needle wash. Three different injection modes are supported; full loop filling (highest precision and reproducibility), partial loop filling (variable volumes, e.g. for dilution series) and µl pickup (loss-free injection of extremely small sample volumes), allowing the user to optimize sample usage. The headspace pressure function prevents bubbles from forming in the vial during sample uptake. This autosampler is also suitable for UHPLC with pressure stability up to 700 or 1240 bar (depending on version) and minimized dead volume for high resolution. A variety of software control options is available: KNAUER offers various options for drivers. Detailed information on page 65. Specifications Sample injection Maximum back pressure Vial/plate dimensions Injection volume range Sample loop Sample capacity see device versions max. plate/vial height: 47 mm (incl. septa or capmat) µl programmable see device versions analytical versions: microtiter plates for max. 768 samples or sample plates for max. 108 vials preparative versions: 24 vials, 10 ml each (LSV) see device versions built-in compressor, only for vials with septum < 100 ms ± 0.6 mm 4-40 C (4-20 C for versions with sample cooling only: AAA21AA, AAA31AA and AAA41AA) Dispenser syringe Headspace pressure Switching time inj. valve Piercing precision needle Sample tray cooling/ heating Vial detection Needle wash Wetted materials Tefzel, Vespel, glass, Teflon (PTFE), standard versions: stainless steel, PEEK; bio versions: PEEK. missing vial/well plate detection by sensor programmable: wash between injections and wash between vials Buffer tubing see device versions Sample loop see device versions Analytical performance Injection modes full loop, partial loopfill and µl pickup mode; PASA (pressure-assisted sample aspiration) Injection precision RSD (Relative Standard Deviation): full loop injection: <0.3%, partial loop injection at an injection volume >10 µl: <0.5% µl pickup injection at an injection volume >10 µl: <1.0% Injection volumes full loop: max µl, partial loop: max µl (50% loop volume) µl pickup: max µl (50% loop volume - 1,5x needle volume) 0,1 µl increment for all injection modes Sample carryover < 0.05% with standard wash; typically < 0.01% with extra wash Injections per vial max. 9 injections Injection cycle time minimum 7 s from the same vial, 14 s from different vials; < 60 s for 100 µl sample injection in all injection modes, incl. 300 µl needle wash Analysis time max. 9 h, 59 min, 59 s Communication Inputs 2 programmable TTL inputs (next injection, freeze, stop) Outputs 1 programmable relay output (inject marker, auxiliary, alarm) Control Ethernet (LAN) Display Mobile Control (optional) General Power supply V AC ± 10%; Hz; 200 VA Dimensions (W x H x D) 300 x 360 x 510 mm cool/heat versions: 300 x 360 x 575 mm Weight 30 kg cool/heat versions: 32 kg Technical parameters Ambient conditions temperature range: 4 40 C; F air humidity: below 90% humidity (non-condensing) 9

16 Autosamplers Device versions Basic Version Basic Basic Cool/Heat Order number AAA00AA AAA01AA Maximum back pressure 700 bar 700 bar Sample needle 15 µl 15 µl Dispenser syringe 250 µl 250 µl Buffer tubing 500 µl 500 µl Sample loop 100 µl, 0.4 mm ID 100 µl, 0.4 mm ID Standard Version Standard Cool/Heat Order number AAA10AA AAA11AA Maximum back pressure 1240 bar 1240 bar Sample needle 15 µl 15 µl Dispenser syringe 250 µl 250 µl Buffer tubing 500 µl 500 µl Sample loop 10 µl, 0.18 mm ID 10 µl, 0.18 mm ID Bio* Version Bio Bio Cool Bio Cool/Heat Order number AAA20AA AAA21AA AAA22AA Maximum back pressure 345 bar 345 bar 345 bar Sample needle 60 µl 60 µl 60 µl Dispenser syringe 250 µl 250 µl 250 µl Buffer tubing 500 µl 500 µl 500 µl Sample loop 100 µl, 0.4 mm ID 100 µl, 0.4 mm ID 100 µl, 0.4 mm ID *biocompatible Prep Version Bio Cool Prep Prep Cool Prep Order number AAA31AA AAA41AA AAA40AA Maximum back pressure 200 bar 200 bar 200 bar Sample needle 60 µl 60 µl 60 µl Dispenser syringe 2500µl 2500µl 2500µl Buffer tubing 2000 µl 2000 µl 2000 µl Sample loop 10 ml 10 ml 10 ml Ordering details: AAA00AA AAA01AA AAA10AA AAA11AA AAA20AA AAA21AA AAA22AA AAA31AA AAA40AA AAA41AA AZURA Autosampler AS 6.1L analytical HPLC autosampler, 700 bar AZURA Autosampler AS 6.1L analytical HPLC autosampler, 700 bar, with sample cooling/heating AZURA Autosampler AS 6.1L analytical HPLC autosampler, 1240 bar AZURA Autosampler AS 6.1L analytical HPLC autosampler, 1240 bar, with sample cooling/heating AZURA Autosampler AS 6.1L analytical HPLC autosampler with biocompatible flow path AZURA Autosampler AS 6.1L analytical HPLC autosampler with biocompatible flow path and sample cooling AZURA Autosampler AS 6.1L analytical HPLC autosampler with biocompatible flow path and sample cooling/heating AZURA Autosampler AS 6.1L preparative HPLC autosampler with biocompatible flow path and cooling option AZURA Autosampler AS 6.1L preparative HPLC autosampler AZURA Autosampler AS 6.1L preparative HPLC autosampler with sample cooling 10

17 Column thermostat AZURA Column Thermostat CT 2.1 The AZURA Column Thermostat CT 2.1 is a price attractive basic column thermostat. It allows temperature control in the range of 5 C and 85 C and thus is appropriate for most HPLC applications. For advanced purification and analysis purposes, the thermostat can optionally be equipped with an eluent pre-heating cartridge. This ensures even more constant separation conditions leading to higher selectivity and an improved peak shape. The instrument operates with a microprocessor controlled Peltier element for precise temperature settings. In combination with its high temperature stability, this allows programming of linear as well as non-linear temperature gradients. A variety of software control options is available: KNAUER offers various options for drivers. Detailed information on page 67. Specifications Thermostatting Heating and cooling system Temperature range 5 85 C Heating/cooling rate microprocessor controlled Peltier element for heating and cooling, fan supported 2-way air circulation 2 C/min Temperature accuracy ± 0.2 C Temperature stability ± 0.1 C Temperature adjustment Column compartment Column dimensions 1 C steps Dimensions, interior 90 x 390 x 47 mm (W x H x D) Safety Leak sensor Optional accessories Communication Interfaces Control General Power supply * total outer dimensions of the column including screw caps self-check and auto-calibration at power-on; selectable turn-off temperature adjustable sensitivity; acoustic signal; turn-off switch Cartridge for eluent pre-heating for capillary with an ID of 0.1 or 0.18 mm LAN Interface optional for stand-alone functionality: Keyboard unit or software V, Hz, 100 W Dimensions 150 x 470 x 310 mm (W x H x D) Weight 8.4 kg max. number max. length* max. outer diameter* matching Vertex Plus column mm 12 mm 125 mm x 4.6 mm ID mm 12 mm 300 mm x 4.6 mm ID mm 35 mm 300 mm x 16 mm ID Ordering details: A05852 AZURA Column Thermostat CT 2.1 with accessories A Cartridge for AZURA Column Thermostat CT 2.1 Eluent Pre-heating ID 0.1 mm A Cartridge for AZURA Column Thermostat CT 2.1 Eluent Pre-heating ID 0.18 mm A Optional keyboard unit for stand-alone functionality of AZURA Column Thermostat CT

18 Diode array detectors AZURA Detector DAD 6.1L The AZURA DAD 6.1L is a high-end diode array detector (DAD) which combines outstanding performance with easy handling. A wide range of easily exchangeable flow cells make this device the right choice for fast, standard analytical, semi-preparative and preparative separations with bio-compatible or stainless steel wetted parts. State-of-the-art total reflection flow cells (LightGuide technology) are available for this detector providing maximum light throughput (due to total internal reflection) with minimal peak dispersion (due to small cell volume) to guarantee an optimized signal to noise (S/N) ratio. An optional fiber optics adapter offers the possibility to separate the flow cell spatially from the device and thus provides enhanced security for hazardous, explosive or toxic work processes, as well as protecting the device from leakages at high flow rates. The newly developed optical unit with KNAUER Polka-Dot technology and intelligent temperature management ensure maximum sensitivity combined with minimal baseline drift over the whole spectrum. Furthermore, easy frontal access and improved safety features enable effortless lamp replacement. This eases maintenance and guarantees short downtimes. The DAD 6.1L comes installed with a high brightness deuterium and tungsten halogen lamps, which cover a wavelength range from 190 to 1000 nm. A variety of software control options is available: KNAUER offers various options for drivers. Detailed information on page 62. Specifications Detection Detector type diode array detector Number of diodes 1024 Detection channels 8 (Digital), 4 (Analog) Light source high brightness deuterium (D2) lamp and halogen lamp with integrated GLP chip Wavelength range nm Spectral bandwidth < 3.5 nm at Hα line (FWHM) Wavelength accuracy ± 1 nm Wavelength verification internal holmium filter and deuterium lines Noise ± 3.5 µau at 254 nm Drift 300 µau/h at 254 nm Linearity > 2.5 AU at 274 nm Time constants 0.0 / 0.01 / 0.02 / / 0.1 / 0.2 / 0.5 / 1.0 / 2.0 / 5.0 / 10.0 s Flow cell not included (see Accessories / Spare parts) Communication Maximum data rate 100 Hz (LAN), 12.5 Hz (analog) Inputs Error (IN), Start (IN), Autozero Outputs Events 1-2, Error (OUT), + 5 V, Valve + 24 V, Valve (OUT) Analog outputs 4 x 0-5 V, 16 bit Control LAN; analog and event controlled Programming wake up Technical parameters GLP detailed report including lamp recognition, operating hours, lamp operating hours, number of lamp ignitions Display Mobile Control (optional) Ambient conditions temperature range: 4-40 C, F humidity: below 90 % non-condensing General Power supply V, Hz, 75 W Dimensions 361 x 158 x 523 mm (W x H x D) Weight 13.8 kg Leak sensor yes Ordering details: ADC11 AZURA Detector DAD 6.1L Diode array detector DAD 6.1L without flow cell nm AMC19XA 10mm, 2µl, 1/16", 50bar LightGuide Flow cell cartridge for AZURA Detector DAD/MWD AMD59XA 50mm, 6µl, 1/16", 50bar High Sensitivity LightGuide Flow cell cartridge for AZURA Detector DAD/MWD AMC38 10mm, 10µl, 1/16", 300bar PressureProof Flow cell cartridge for AZURA Detector DAD/MWD AMB18 3mm, 2µl, 1/16", 300bar PressureProof Flow cell cartridge for AZURA Detector DAD/MWD AZL01 Deuterium lamp type HBST for AZURA Detector DAD 6.1L AZL02 Halogen replacement lamp for AZURA Detector DAD 6.1L AMKX8KIT Fiber optics adapter kit for AZURA Detector DAD/MWD AMLX8 Test cell for AZURA Detector DAD/MWD 12

19 Diode array detectors AZURA Detector DAD 2.1L & MWD 2.1L The AZURA Detector DAD 2.1L is a highly competitive diode array detector which combines high performance with easy handling at an affordable price. A wide range of easily exchangeable cartridge flow cells make this device the right choice for fast, standard analytical, semi-preparative and preparative separations with bio-inert or stainless steel wetted parts. State-of-the-art total reflection flow cells (LightGuide technology) are available for this device providing maximum light throughput (due to total internal reflection) with minimal peak dispersion (due to the small cell volume) to guarantee an optimized S/N ratio. An optional fiber optics adapter offers the possibility to separate the flow cell spatially from the device and thus provides enhanced security for hazardous, explosive or toxic work processes, as well as protecting the device from leakages at high flow rates. The newly developed optical unit and intelligent temperature management ensure for maximum sensitivity combined with minimal baseline drift. Furthermore, easy frontal access and improved safety features enable effortless lamp replacement. This eases maintenance and guarantees short downtimes. The device comes installed with a deuterium lamp which covers a wavelength range from 190 to 700 nm. A variety of software control options is available: KNAUER offers various options for drivers. Detailed information on page 62. Specifications Detection Detector type DAD 2.1L, diode array detector / MWD 2.1L, multi-wavelength detector Number of diodes 256 Detection channels 8 (Digital), 4 (Analog) Light source deuterium (D2) lamp with integrated GLP chip Wavelength range nm Spectral bandwidth < 10 nm at Hα line (FWHM) note: digital bandwidth 1-32 nm Wavelength accuracy ± 1 nm Wavelength verification internal holmium filter and deuterium lines Noise ± 5 µau at 254 nm Drift 300 µau/h at 254 nm Linearity > 1.6 AU at 274 nm Time constants 0.0 / 0.1 / 0.2 / 0.5 / 1.0 / 2.0 / 5.0 / 10.0 s Flow cell not included (see Accessories / Spare parts) Communication Maximum data rate 100 Hz (LAN) 12.5 Hz (Analog) Inputs Error (IN), Start (IN), Autozero Outputs Events 1-2, Error (OUT), + 5 V, Valve + 24 V, Valve (OUT) Analog outputs 4 x 0-5 V, 16 bit Control LAN; analog and event controlled Programming wake up Technical parameters GLP detailed report including lamp recognition, operating hours, lamp operating hours, number of lamp ignitions Display Mobile Control (optional) Ambient conditions temperature range: 4-40 C, F humidity: below 90 % noncondensing General Power supply V, Hz, 75 W Dimensions 361 x 158 x 523 mm (W x H x D) Weight 12.8 kg Leak sensor yes Ordering details: ADC01 ADB01 AMB18 AMC19XA AMD59XA AMC38 AZURA Detector DAD 2.1L Diode array detector DAD 2.1L without flow cell nm AZURA Detector MWD 2.1L Multiwavelength detector MWD 2.1L, without flow cell nm 3mm, 2µl, 1/16", 300bar PressureProof Flow cell cartridge for AZURA Detector DAD/MWD 10mm, 2µl, 1/16", 50bar LightGuide Flow cell cartridge for AZURA Detector DAD/MWD 50mm, 6µl, 1/16", 50bar High Sensitivity LightGuide Flow cell cartridge for AZURA Detector DAD/MWD 10mm, 10µl, 1/16", 300bar PressureProof Flow cell cartridge for AZURA Detector DAD/MWD A5193 Deuterium lamp, replacement, for S2550, S2520, 10D 40D, 50D, UVD 2.1S, UVD 2.1L DAD 2.1L, MWD 2.1L AMKX8KIT AMLX8 Fiber optics adapter kit for AZURA Detector DAD/MWD Test cell for AZURA Detector DAD/MWD 13

20 UV/VIS detectors AZURA Detector UVD 2.1L Variable single wavelength The AZURA Detector UVD 2.1L is a competitively priced HPLC spectrophotometer for routine HPLC applications including fast LC methods. Besides offering excellent technical specifications, this robust detector features a highly flexible and compact design. The UVD 2.1L comes with an installed deuterium lamp which covers a wavelength range from 190 to 750 nm. Due to a smart design the flow cell is easily accessible and can be changed very quickly. You can choose between a wide range of flow cells for analytical or preparative LC applications with flow rates from 10 μl/min up to 10 l/min. A variety of software control options is available: KNAUER offers various options for drivers. Detailed information on page 62. Specifications Detection Detector type variable single wavelength UV/VIS detector Detection channels 1 Light source deuterium (D2) lamp with integrated GLP chip Wavelength range nm Spectral bandwidth 11 nm at Hα line (FWHM) Wavelength accuracy ± 2.5 nm Wavelength precision 0.3 nm (ASTM E ) Noise ± 15 µau at 254 nm (ASTM E ) Drift 300 µau/h at 254 nm (ASTM E ) Linearity > 2.0 AU at 270 nm (ASTM E ) Time constants 0.0 / 0.1 / 0.2 / 0.5 / 1.0 / 2.0 / 5.0 / 10.0 s Flow cell not included (see Accessories / Spare parts) Communication Maximum data rate 50 Hz (LAN) 20 Hz (analog) Inputs Error (IN), Start (IN), Autozero, 1-10 V Analog IN Outputs Events 1-3, + 5 V, 24 V Valve Analog outputs 1 x 0-5 V scalable, 16 bit, offset adjustable Control digital: LAN; RS-232; analog and event controlled analog: wavelength control manual: Mobile Control (optional) Programming timed: wavelength, events, fraction valve, links, wake up (program, link); 9 programs, 50 program lines Technical parameters GLP detailed report including lamp recognition, operating hours, lamp operating hours, number of lamp ignitions Display Mobile Control (optional) Ambient conditions temperature range: 4-40 C, F humidity: below 90 % noncondensing General Power supply V, Hz, 75 W Dimensions 361 x 158 x 523 mm (W x H x D) Weight 5.9 kg Leak sensor yes Ordering details: ADA01XA AZURA Detector UVD 2.1L with deuterium lamp without flow cell ADA04XA AZURA Detector UVD 2.1L Fiber Optics Version with deuterium lamp without flow cell A4061XB Flow cell UV, 10 mm 10 µl, 1/16", stainless steel, with heat exchanger one sided inlet and outlet A4042 Flow cell UV, 3 mm, 2 µl, 1/16", stainless steel A5193 Deuterium lamp, replacement, for S2550, S2520, 10D 40D, 50D, UVD 2.1S, UVD 2.1L DAD 2.1L, MWD 2.1L A4126 Test cell Holmium Oxid Filter A4146 Test cell, WG 280 filter stray light A4123 Test cell 14

21 UV/VIS detectors BlueShadow UV/VIS Detector 50D Variable multiple wavelength The BlueShadow 50D offers high sensitivity and baseline stability for HPLC applications including fast LC methods. With its high speed scanning design, up to four different wavelengths can be monitored simultaneously while acquiring spectral data from 190 to 750 nm. Its remarkable values for noise, drift, and linearity are achieved through smart optical bench technology and the use of optimized components. The detector comes installed with a deuterium lamp which covers a wavelength range from 190 to 750 nm. Due to a smart design the flow cell is easily accessible and can be changed very quickly. A wide range of flow cells is available for practically any LC application, including micro flow cells for flow rates up to 10 μl/ min and flow cells for preparative HPLC up to 10 l/min. A variety of software control options is available: KNAUER offers various options for drivers. Detailed information on page 62. Specifications Detection Detector type variable multiple wavelength UV/VIS detector Detection channels 4 Light source deuterium (D2) Wavelength range nm Spectral bandwidth 6 nm at Hα line (FWHM) Wavelength accuracy ± 2.0 nm (verification with integrated holmium oxide filter) Wavelength precision 0.4 nm (ASTM E275-93) Noise ± 7.5 μau at 254 nm (ASTM E ) Drift 150 μau/h at 254 nm (ASTM E ) Linearity > 2.2 AU at 270 nm (ASTM E ) Time constants 0.1 / 0.2 / 0.5 / 1.0 / 2.0 / 5.0 / 10.0 s Flow cell not included Integration time automatic Communication Maximum data rate Inputs Outputs Analog outputs Control Programming Technical parameters 80 Hz (LAN) 1 channel 20 Hz (Analog) 1 channel 10 Hz (RS 232) 1 channel Error (IN), Start (IN), Autozero, 0-10 V Analog IN Events 1-3, + 5 V, 24 V Value 2 x 0-5 V scalable, 20 bit, offset adjustable digital: LAN; RS-232; analog and event controlled analog: wavelength control manual: touchpad timed: wavelengths, events, fraction valve, links, wake up (program, link); 9 programs, 50 program lines GLP detailed report including lamp recognition, operating hours, lamp operating hours, step motor operating hours, number of lamp ignitions Display touchscreen TFT 2.4" Ambient conditions temperature range: 4 40 C; F humidity: below 90% noncondensing General Power supply V, Hz, 75 W Dimensions 242 x 169 x 399 (W x H x D) Weight 5.3 kg Ordering details: C55190XA.0 UV-Detector BlueShadow 50D, flow cell excluded, accessories included A4061XB Flow cell UV, 10 mm 10 µl, 1/16", stainless steel, with heat exchanger one sided inlet and outlet A4042 Flow Cell UV, 3 mm, 2 µl, 1/16", stainless steel A5193 Deuterium lamp, replacement, for S2550, S2520, 10D 40D, 50D, UVD 2.1S, UVD 2.1L DAD 2.1L, MWD 2.1L A4126 Test cell Holmium Oxid Filter A4146 Test Cell, WG 280 filter stray light A4123 Test cell 15

22 UV/VIS detectors AZURA Detector UVD 2.1S Small variable single wavelength The AZURA UVD 2.1S is a highly competitive single variable wavelength UV detector for HPLC and purification applications such as Prep LC and FPLC. It offers excellent technical specifications for routine laboratory work. With its small footprint, it is one of the smallest detectors for HPLC on the market. The UVD 2.1S comes in the novel small AZURA housing. The installed deuterium lamp covers a wavelength range from 190 to 500 nm. Due to a smart design the flow cell is easily accessible and can be changed very quickly. You can choose between a wide range of flow cells for analytical or preparative LC applications with flow rates from 10 μl/min up to 10 l/min. A variety of software control options is available: KNAUER offers various options for drivers. Detailed information on page 62. Specifications Detection Detector type small variable single wavelength UV detector Detection channels 1 Light source deuterium (D2) lamp with integrated GLP chip Wavelength range nm Spectral bandwidth 13 nm at Hα line (FWHM) Wavelength accuracy ± 3 nm Wavelength precision 0.7 nm (ASTM E ) Noise 20 µau at 254 nm (ASTM E ) Drift 300 µau/h at 254 nm (ASTM E ) Linearity > 2.0 AU at 270 nm (ASTM E ) Time constants 0.00 / 0.02 / 0.05 / 0.1 / 0.2 / 0.5 / 1.0 / 2.0 s Flow cell not included (see Accessories / Spare parts) Communication Maximum data rate Inputs Outputs Analog outputs Control Technical parameters GLP Display Ambient conditions General 50 Hz (LAN, Analog) 10 Hz (RS-232) Autozero, Start (IN), Error (either IN or OUT), 1-10 V Analog IN Error (either OUT or IN) 1 x ± 2.5 V scalable, 20 bit digital: LAN; RS-232; analog and event controlled analog: wavelength control manual: front panel or software lamp operating hours LED or Mobile Control (optional) temperature range: 4-40 C, F humidity: below 90% noncondensing Power supply external: input V, output 24 V DC, 60 W Dimensions 121 x 129 x 187 mm (W x H x D) Weight 1.5 kg Ordering details: ADA00 AZURA Detector UVD 2.1S with deuterium lamp without flow cell ADA05 AZURA Detector UVD 2.1S Fiber Optics Version with deuterium lamp without flow cell A4061XB Flow cell UV, 10 mm 10 µl, 1/16", stainless steel, with heat exchanger one sided inlet and outlet A4042 Flow Cell UV, 3 mm, 2 µl, 1/16", stainless steel A5193 Deuterium lamp, replacement, for S2550, S2520, 10D 40D, 50D, UVD 2.1S, UVD 2.1L DAD 2.1L, MWD 2.1L 16

23 Refractive index detector AZURA Detector RID 2.1L Analytical refractive index detector The AZURA RID 2.1L is a sensitive and competitively priced differential refractometer suitable for detecting compounds with little or no UV activity such as alcohols, sugars, lipids or polymers. This instrument is designed for use in analytical HPLC (high performance liquid chromatography) as well as for GPC (gel permeation chromatography) applications. The intelligently designed optical unit with advanced temperature control ensures high sensitivity, fast baseline stabilization, and excellent reproducibility. Furthermore, the long-life LED, highly pressure resistant flow cell, improved safety features and enhanced diagnostics functions guarantee easy handling and minimal maintenance. The wide linear dynamic range and 10 ml/min maximum flow rate make the AZURA RID 2.1L the perfect choice for most laboratory tasks. A variety of software control options is available: KNAUER offers various options for drivers. Detailed information on page 62. Specifications Detection Detector type analytical refractive index detector Light source long-life LED Refractive index range RIU Noise ± 2.5 nriu Drift 200 nriu/h Linearity > 1000 µriu Temperature control OFF, C (1 C increment) Maximum flow rate 10 ml/min (pure water) Time constants 0.00 / 0.01 / 0.02 / 0.05 / 0.1 / 0.2 / 0.5 / 1.0 / 2.0 / 5.0 / 10.0 s Autozero full range Flow cell 5 bar back pressure resistence flow cell included Flow cell volume 15 μl Wetted materials stainless steel / fused silica / PTFE / PEEK Communication Maximum data rate 100 Hz (LAN) 20 Hz (Analog) Inputs Error (IN), Start (IN), Autozero, Flush (IN) Outputs Event 1, Start (OUT), Error (OUT), + 5 V, 24 V Valve Analog outputs 1 x V scalable, 20 bit, offset adjustable Control digital: LAN; analog and event controlled manual: software Technical parameters GLP detailed report including operating hours, light source operating hours Display Mobile Control (optional) Ambient conditions temperature range: 4-40 C, F humidity: below 90 % noncondensing General Power supply V, Hz, 60 W Dimensions 361 x 158 x 523 mm (W x H x D) Weight 10.8 kg Leak sensor yes (internal and external leak management) Optional accessories Mobile Control Ordering details: ADD31 AZURA Detector RID 2.1L Refractive Index Detector with flow cell 17

24 Fluorescence detector Fluorescence Detector RF-20A/Axs The fluorescence detector RF-20Axs provides world-class sensitivity, excellent maintainability and diverse validation / support functions. It supports a wide range of applications in the wavelength range of 200 to 750 nm from conventional analysis to high-performance analysis. With a signal-to-noise ratio of 2000 for the water-raman band, the fluorescence detector is very well suited for trace analysis. Measuring cell tempering ensures the lowest possible noise and drift values. The integrated low-pressure mercury lamp allows easy wavelength accuracy verification. The xenon lamp and flow cell are directly accessible on the device, thus allowing a quick and easy handling and maintenance of the device by the user, thereby minimizing downtime. The lamp life is 2000 hours. When replacing the xenon lamp, no adjustment is required. A variety of software control options is available: KNAUER offers various options for drivers. Detailed information on page 62. Specifications Detection Detector type Detection channels 2 Number of signals 2 Light source Wavelength range Spectral bandwidth Wavelength accuracy Wavelength precision spectrofluorometric detector xenon lamp, low-pressure; mercury lamp for wavelength accuracy check (RF-20 Axs only) 200 to 750 nm RF-20 Axs / 200 to 650 nm RF-20 A 20 nm ± 2 nm ± 0.2 nm Indicates the precision performance when the power is turned ON in single wavelength mode and the wavelength is changed. Sensitivity can be set at three levels: HIGH (x 1), MED (x 32), LOW (x 1024) Wetted materials Flow cell volume 12 µm Time constants Autozero Heated zone Temperature range Communication SUS316L, PTFE (fluorocarbon polymers), quartz 11 levels can be selected, equivalent to "no filter", 0.05, 0.1, 0.5, 1.0, 1.5, 2.0, 3.0, 6.0, 8.0 and 10.0 seconds auto zero function, basline shift function room temperature (ambient temperature) -10 C up to 40 C (at flow rates less than 2 ml/min, column oven temperatures less than 85 C, only for RF-20Axs) Gain can be set at three levels: x 1, x 4, x 16 Technical parameters Ambient conditions General Power supply operating temperature: 4 to 35 C, relative humidity: 20 to 85 % (no condensation) AC V, 400 VA, 50/60 Hz Dimensions 260 x 210 x 420 mm (W x H x D) Weight 18 kg Ordering details: A59200 Fluorescence detector RF-20 A nm incl. accessories A59201 Fluorescence detector RF-20 Axs nm incl. accessories A59210 Xenon lamp for RF-20A/Axs A59211 Flow cell for Semi micro LC cell capacity 3 µl, supports temperature control (RF-20Axs only) A59212 Inert flow cell for RF-20A/Xs, cell capacity 12 µl, contact materials: PEEK, PTFE A59213 Photomultiplier f. RF-20A/Axs, wavelength 200 nm to 900 nm A59300 CBM-20A Interface CE/IVD PC Interface (request software control option via KNAUER) A Shimadzu LC Driver for Chromeleon 7.2 Shimadzu CBM-20A required 18

25 Light scattering detector Light scattering detector Sedex LC/85LT/90LT Universal ELSD detection for HPLC Evaporative Light Scattering Detection (ELSD) is a universal modern technology with which every analyte that is less volatile than the mobile phase can be detected. Using the unique Low Temperature technology, this detector allows the achievement of very high sensitivity. The technology is gradient compatible and is independent of the absorption characteristics of the eluents. Compounds can be universally measured with this detector (carbohydrates, proteins, peptides, polymers, lipids, steroids, etc.), regardless of their fluorescence, absorption or refractive-index characteristics. Comprehensive SOP protocols for GLP conformity and validation procedures are available. A variety of software control options is available: KNAUER offers various options for drivers. Detailed information on page 62. Specifications Detection Detector type Light source Sensitivity Maximum data rate Nebulizer Gas Gas flow rate Gas inlet pressure HPLC flow rate (85 LT) Maintenance Heated zone Temperature range ambient to 100 C Communication high sensitivity photomultiplier selected high effciency blue LED (470 nm), elapsed-time counter < 5 ng caffein (LOD) for LC / < 1 ng caffein (LOD) for 85LT / < 0.5 ng stearic a analog: 40 Hz, analog: 100 Hz 85LT, digital: 30 Hz nitrogen preferred < 3 l/min 3.5 bar (51 psi) HPLC nebulizer: 200 µl/min 2 ml/min UHPLC nebulizer: 200 µl/min 1.4 ml/min low flow nebulizer: 35 µl/min 900 µl/min micro nebulizer: 4-40 µl/min easily accessible from the front for cleaning Gain factor 1 to 2048 in 12 steps (2 1 to 2 11) Filter moving average (0 10 seconds) Analog outputs 0 1 V Analog control input contact closure for ready, auto-zero Control RS-232 Power-down methods shut-off: gas, LED, heating and/or PMT cleaning mode Technical parameters Display General liquid crystal Power supply 230 V/50 Hz, 1.7 A; 115 V/60 Hz, 1.8 A Dimensions 250 mm x 480 mm x 550 mm (W x H x D) Weight 18.5 kg Ordering details: A Sensitive Light scattering detector ELSD 85LT for univ. detection ml/min, 100 Hz including accessories A High Sensitive Light scattering detector ELSD 90LT for univ. detection ml/min, 100 Hz including accessories A Preparative Light scattering detector ELSD SEDEX LC for univ. detection 200 µl/min - 2 ml/min A OpenLAB CDS EZChrom Edition drivers Sedex 80/85/90LT/LC not for fraction collection A Sedex Driver for Chromeleon 7.2; For Sedex 85LT / 90LT / FP / LC / 100LT; Instrument Controler Class 3 necessary A0754INST Installation ELSD including IQ, OQ and PQ for Germany, Switzerland and Austria 19

26 Electrochemical detector Electrochemical Detector DECADE Elite SCC Single cell control/dual cell control This detector enables you to perform all applications using electrochemical detection. The DECADE II includes a highly stable Faraday-shielded oven compartment accommodating column and flow cell. This flow cell has surprised researchers for its unsurpassed S/N ratio and provides best possible combination for extremely sensitive EC analyses. The DECADE II covers the DC, pulse and scan mode. The DC mode covers about 90% of all applications. The pulse mode is important for PAD (Pulsed Amperometric Detection) of for example carbohydrates. The scan mode is used to obtain a voltammogram in method optimization. Important parameters in the DC and pulse mode can be changed on a time base by user-defined commands, which enables maximum control to fully automate the detection. In addition, crucial parameters can be controlled by either relays or TTL. For highest sensitivity, the SenCell is recommended with carbon electrode with a 5 year warranty. A variety of software control options is available: KNAUER offers various options for drivers. Detailed information on page 62. Key features highest sensitivity down to the low fentomol range (on column) excellent precision due to thermostatically controlled Faraday s cage for measuring cell and column flexible and efficient due to control of up to 4 cells wide selection of working and reference electrodes optimized signal to noise ratio via ADF (advanced digital filter) can be updated using flash-technology well-arranged display, screw connection for sample feed at the front Specifications Detection Detector type electrochemical detector (DC, PAD and scan operating modes) Outputs between ± 1 V and ± 10 V (20 bit D/A converter) Autozero triggered via keypad, TTL or RS-232 Control RS-232, data transmission with 1, 2, 5 or 10 Hz Dimensions 220 x 440 x 440 mm (W x H x D) Weight 14 kg Ordering details: A07545 Electrochemical detector DECADE Elite SCC without flow cell AZB00XA AZURA Interface Box IFU 2.1 LAN, A/D converter, 4 channels A07546 Flow cell SenCell 2 mm GC Salt-Bridge A Flow cell FlexCell Au HyREF Flow Cell A SenCell GC HyREF Flow Cell 20

27 Conductivity detector Conductivity detector CDD-10 Avp The CDD-10AVP is a highly sensitive conductivity detector applicable to ion chromatograph or organic acid analysis. Low noise, low drift and wide dynamic range assure proven performance of the CDD-10AVP detector. A special features is the VP key for validation. Flow cell 0.25 µl included. Further flow cells on request. A variety of software control options is available: KNAUER offers various options for drivers. Detailed information on page 62. Specifications Detection Noise Level Drift (Time) Drift (Temperature) Ambient Temperature Response Zero Adjustment Porality Measurement Range Measurement Sensitivity Gain Range Display Output Recorder Integrator Cell Temperature Control Power Requirements Dimensions less than 4 nscm-1 (BG: F285 µscm-1) less than 25 nscm-1 (BG: F285 µscm-1) less than 25 nscm-1 (BG: F285 µscm-1) 4-35 deg. C time constant: 0.05, 0.1, 0.5, 1.0, 1.5, 2.0, 3.0, 6.0, 8.0, 10.0 sec, 10 steps auto zero, baseline shift positive and negative ,200 µscm-1 FS 0.01, 0.1, 1, 10, 100 µscm-1 FS/mV, 5 steps 1-512, SHORT LCD with Back-lit 10 mv recorder terminal 100, 10, 1, 0.1, 0.01 mv/µscm µl volme, 2.9 MPa max. pressure, SUS316, PEEK, Teflon deg. C (1 deg. C step), OFF AC100 - AC240 V, 250 VA W 260 mm x D 420 mm x H 140 mm Ordering details: A Conductivity detector CDD-10 Avp with flow cell 0.25 µl AZB00 AZURA Interface Box IFU 2.1 USB 21

28 Conductivity/pH monitor AZURA Conductivity Monitor CM 2.1S Conductivity monitor with optional ph measurement The AZURA CM 2.1S is a reliable conductivity monitor which is utilized in FPLC to follow salt gradients. Optionally ph values can be measured. Wetted parts are completely biocompatible. The contactless measurements of conductivity reduces the risk of carryover to a minimum. Two flow cells cover a flow rate up to either 10 ml/min or 100 ml/min and are optimized for low dead volume. Please order conductivity flow cells and the ph measuring kit in addition to the CM 2.1S. A variety of software control options is available: KNAUER offers various options for drivers. Detailed information on page 62. ADG30 Key features contactless conductivity measurement fast response high accuracy and reproducibility, low noise accurate and reliable monitoring through self-test and self-calibration short capillaries for minimizing band broadening and time delay between detectors measurement kit is optional and has to be separately ordered optional upgrade for ph measurement is possible A70091 Specifications Detection Flow cell 10 ml or 100 ml Detector type conductivity Measurement range 1 µs/cm 999 ms/cm Drift temperature sensor ± 0.3 C per 10 h ph measurement 2-12 Communication Maximum data rate 5 Hz Supported electrodes conductivity: automatic recognition ph: all ph electrodes with BNC connector (compatible) and available flow cell Autozero automatic Measurement accuracy temperature sensor accuracy: ± 1.0 C; conductivity accuracy: < 2% full scale or ± 5 µs/cm whichever is greater in the range 1 µs/cm 300 ms/cm Analog outputs 2 channels DAC 18 bit conductivity value ph Value (only for the stand-alone device) Digital outputs LAN; RS-232 Technical parameters GLP electronic Serial No. Display LCD, 2 x 8 characters Ambient conditions operating temperature: 15 to 35 C, relative humidity: 45 to 85% General Power supply V, Hz, max. 20W Dimensions 121 x 129 x 187 mm (W x H x D) Ordering details: ADG30 A4156 A4157 A70091 A5813 AZURA Conductivity Monitor CM 2.1S without flowcell AZURA Conductivity Monitor CM 2.1S - flowcell for flow rates up to 10 ml/min 160 bar, ID 0,25 mm AZURA Conductivity Monitor CM 2.1S - flowcell flow rates up to 100ml per min 100 bar, ID 0,75 mm ph measuring kit complete for conductivity monitor up to 80 ml/min Flow splitter for use of CM 2.1S over 100 ml/min 22

29 Fraction collectors Foxy R1 and Foxy R2 The Foxy R1 and Foxy R2 are automated fraction collectors for robust and reliable collection of fractions and can be used universally in research laboratories, for routine investigations, for analytical tasks, for semi-preparative separations and for bio compatible applications. The tworack Foxy R2 fraction collector uses radio frequency identification to automatically detect collection racks for easy setup. A variety of software control options is available: KNAUER offers various options for drivers. Detailed information on page 68. Key features: Collect uniform fractions by time or pumped volume. Collect peaks of interest separately using any combination of slope, level, and time. Stores up to 8 collection methods. Choice of available racks Cooling option available (special cooling rack, cooling option and thermostat necessary) Coldroom-storage possible Easy to use symbolic touch screen interface Fast tube changes Small delay volume between diverter valve and drop former Specifications Fraction collector Foxy R1/R2 Number of racks Foxy R1: 1 Foxy R2: 2 RFID rack recognition Foxy R1: no Foxy R2: yes Capillary connection 1/16, 1/8 or 1/4 Fractionation modes drop counting, time intervals, volume intervals Fractionation flow rate Foxy R1: max. 125 ml/min (1/16 : up to 25 ml/min) Foxy R2: max 1000 ml/min (1/8 : up to 125 ml/min) Diverter valve drop former (NC): 110 µl waste (NO): 130 µl Wetted materials valve: PEEK and perfluoroelastomer (FFKM), supplied ferrules: ETFE, supplied valve tubing: PTFE, supplied drain tubing: vinyl Fractionation control operator: front panel control via touch screen LCD integrated systems: direct communication via Ethernet (TCP/IP) and RS-232 serial communications Maximum test tube height Foxy R1: 160 mm, Foxy R2: 196 mm, Foxy R2 1/4 : 180 mm Cooling option Special cooling racks, cooling option and thermostat necessary Communication Control LAN, RS-232 Technical parameters Conformity CE, CSA Display touch screen LCD displays Ambient conditions 0 40 C, F General Power supply VAC, Hz, max. 1 A Dimensions Foxy R1: 31.1 x 35,5 x 33 mm (W x D x H) Foxy R2: 31.1 x 37,8 x 53,3 mm (W x D x H) Foxy R2 1/4 : 31.1 x 39,4 x 53,3 mm (W x D x H) Weight Foxy R1: 7.1 kg, Foxy R2: 10.3 kg, Foxy R2 1/4 : 10.4 kg Ordering details: A59100 A59102 A Fraction collector Foxy R1, for 1/16 or 1/8 tubing Fraction collector Foxy R2, for 1/8 tubing Fraction collector Foxy R2, for 1/4 tubing 23

30 Fraction collectors Racks for Foxy R1/R2 Specification: Article number max volume (ml) vessel outer diameter (mm) vessel length (mm) Rack Foxy R1 capacity Foxy R2 capacity A mm test tubes A mm test tubes A mm test tubes A mm test tubes A mm test tubes A mm test tubes A ml Falcon tubes A mm test tubes A mm test tubes n/a 144 A mm test tubes A mm test tubes n/a 72 A , ml mirco-centrifuge or Eppendorf tubes A n/a n/a 96-well microplates 2 4 or 6 A ml Falcon tubes A bottle 9 18 A59109 unlimited n/a n/a funnel or 72 A59101 unlimited n/a n/a funnel n/a 26 A ml mirco-centrifuge or Eppendorf tubes 144 n/a A ml Falcon tubes 72 n/a A n/a n/a 96-well microplates 2 n/a Ordering details: A59111 Rack for 2 microwell plates 96 well for fraction collector Foxy R1/R2 A59110 Rack for 36 falcons 50 ml for fraction collector Foxy R1/R2 A59109 Prep. funnelrack for Foxy R1R2 36 places with vinyl tubing A59101 Prep. funnelrack for Foxy R2 26 places with vinyl tubing A59108 Rack for 36 vials 25 mm for fraction collector Foxy R1/R2 A59107 Rack for 60 tubes 1.5 ml for fraction collector Foxy R1/R2 A59106 Rack for 72 falcons 15 ml for fraction collector Foxy R1/R2 A59105 Rack for 100 vials 16 mm for fraction collector Foxy R1/R2 A59104 Rack for 144 vials 12 mm for fraction collector Foxy R1/R2 A59103 Rack for 144 vials 12 mm for fraction collector Foxy R1/R2 A59114 Racks for 2 x 9 bottles 480 ml for fraction collector Foxy R2 A59112 Funnel trays for fraction collector Foxy R1/R2 (10 pieces) A59117* Cooling rack for 144 tubes 1.5 ml for fraction collector Foxy R1 with cooling A59118* Cooling rack for 72 falcons 15 ml for fraction collector Foxy R1 with cooling A59119* Cooling rack for microwell plates for fraction collector Foxy R1 with cooling A59122 Cooling option with cooling hood, cooling plate and accessories A70050 Thermostatting unit -40 to 200 C A70055 Thermostatting unit -20 to 40 C *For Foxy R1 with cooling option 24

31 Fraction collectors LABOCOL Vario-4000 The LABOCOL Vario-4000 fraction collectors are characterized by their small dimensions combined with an optimal space utilization. The open system without limitation of the fractions and vessel sizes can be tailored to individual needs. The fraction collectors of the LABOCOL Vario-4000 series offer the corresponding program for each application and are flexible to handle. The versatile application possibilities make the Vario-4000 series ideal for use in research and development as well as in production. The individual Vario-4000 models differ in the base area and the flow rate range. A variety of software control options is available: KNAUER offers various options for drivers. Detailed information on page 68. Key features: Collect uniform fractions by time or pumped volume Choice of available racks Space for three or five different racks Waste / fraction valve for 1/16, 1/8 or 1/4 tubing Flow rates up to 1000 ml/min Specifications LABOCOL Vario 4000 and Vario-4000 Plus Number of racks Vario-4000: 3 racks Vario-4000 Plus: 5 racks Capillary connection 1/16, 1/8 or 1/4 Fractionation flow rate up to 1000 ml/min Wetted materials PPEK and PTFE Communication Control LAN, RS-232 General Power supply VAC, Hz, max. 2.5 A Dimensions Vario-4000: 30 x 50 x 52 mm (W x D x H), Maximum footprint for racks: 24 x 41 (W xd) Vario 4000 Plus: 46 x 50 x 52 mm (W x D x H), Maximum footprint for racks: 40 x 41 (W xd) Weight Vario-4000: 8 kg Vario-4000 Plus: 10 kg Ambient conditions 0 40 C, F Rack specifications: Article number Max volume (ml) Vessel outer diameter (mm) Rack Capacity vials per rack Max vials Vario-4000 Max vials Vario-4000 Plus A Standard rack (3 racks à 80 vials) 400 (5 racks à 80 vials) A Micro rack (3 racks à 125 vials) 625 (5 racks à 125 vials) A / Prep rack (3 racks à 60 vials) 300 (5 racks à 60 vials) A Semiprep rack (3 racks à 39 vials) 195 (5 racks à 39 vials) A Falcon rack (3 racks à 24 vials) 120 (5 racks à 24 vials) Ordering details: A A A A A59130 A59131 A59132 A59133 A59134 Fraction collector LABOCOL Vario-4000, for 1/16 or 1/8 tubing Fraction collector LABOCOL Vario-4000, for 1/4 tubing Fraction collector LABOCOL Vario-4000 Plus, for 1/16 or 1/8 tubing Fraction collector LABOCOL Vario-4000 Plus, for 1/4 tubing Standard rack for fraction collector LABOCOL Vario-4000 / Vario-4000 Plus Micro rack for fraction collector LABOCOL Vario-4000 / Vario-4000 Plus Prep rack for fraction collector LABOCOL Vario-4000 / Vario-4000 Plus Semiprep rack for fraction collector LABOCOL Vario-4000 / Vario-4000 Plus Falcon rack for fraction collector LABOCOL Vario-4000 / Vario-4000 Plus 25

32 Electrical valves AZURA Valve Drive V 2.1S Multiposition Multi-position valves are used for fraction collection, eluent selection or column switching. They have one port in a central position and one channel which connects the central port to one of the peripheral ports. If more than one valve is needed for your application, it might be helpful two combine these as units in one stackable assistant ASM 2.1L. A variety of software control options is available: KNAUER offers various options for drivers. Detailed information on page 67. Specifications Control LAN, RS-232, analog, keypad Dimensions 105 x 100 x 185 mm (W x H x D), without valve Display LCD or Mobile Control (optional) Power supply external DC24V, 60 W Ambient conditions temperature range: 4 40 C; F air humidity below 90% humidity (non-condensing) General Dimensions valve diameter 47.5 mm Weight 1.86 kg, without valve AWA30BH A5854 Variants valve drive valve valve material connection bore size max. flowrate 6-port 10-port 12-port 16-port none AWA10 n/a AWA20 AWA30 multiposition stainless steel / Vespel 1/16" 0.3 mm 50 ml/min AWA11DA 400 bar column selection valve for 5 columns, bypass option and reverse flow stainless steel / Vespel or POM-H-TF 1/16" 0.6 mm 100 ml/min AWA10BA 300 bar PEEK / PPS 1/16" 0.6 mm 50 ml/min AWA10BB 200 bar stainless steel / Vespel or POM-H-TF 1/8" 1.2 mm 500 ml/min AWA10BC 300 bar PEEK / PPS 1/8" 1.2 mm 500 ml/min AWA10BD 200 bar stainless steel / Valcon E2 n/a n/a n/a n/a n/a AWA30BH 50 bar n/a n/a n/a n/a 1/4" 4.0 mm 1000 ml/min n/a A bar PEEK / Valcon E2 1/4" 3.9 mm 1000 ml/min A bar AWA20BG 25 bar n/a n/a n/a n/a n/a n/a n/a n/a n/a PEEK / Valcon E 1/16" 0.4 mm 50 ml/min n/a n/a AWB00FC 50 bar n/a Ordering details: AWA11DA AZURA Valve Drive V 2.1S with 6-port multiposition valve, stainless steel, 1/16, 400 bar AWA10BA AZURA Valve Drive V 2.1S with 6-port multiposition valve, stainless steel, 1/16", 300 bar AWA30BH AZURA Valve Drive V 2.1S with 16-port multiposition valve, stainless steel, 1/16, 50 bar AWA10BB AZURA Valve Drive V 2.1S with 6-port multiposition valve, PEEK, 1/16" AWA10BC AZURA Valve Drive V 2.1S with 6-port multiposition valve, stainless steel, 1/8" AWA20BG AZURA Valve Drive V 2.1S with 12-port multiposition valve, stainless steel, 1/8, 25 bar AWA10BD AZURA Valve Drive V 2.1S with 6-port multiposition valve, PEEK, 1/8, 150 bar A5850 VICI/VALCO Selector with 10-port mutliposition valve, stainless steel, 1/4, 7 bar A5854 VICI/VALCO Selector with 6-port multiposition valve, PEEK, 1/4, 17 bar AWB00FC Column Selection Valve VICI for 5 columns, 1 bypass, reverse flow, 12 ports, 1/16, 50 bar 26

33 Electrical valves Electrical valves for injections and/or switching tasks 2-position valves are used mostly for injections, but can be used for complex switching systems as well. All ports are located in peripheric positions. The channels always connect two of these ports. If more than one valve is needed for your application, it might be helpful to combine these as units in one stackable assistant ASM 2.1L. A variety of software control options is available: KNAUER offers various options for drivers. Detailed information on page 67. Specifications valve drives Control LAN, RS-232, analog, keypad Dimensions 105 x 100 x 185 mm (W x H x D), without valve Display LCD or Mobile Control (optional) Power supply external DC24V, 60 W Ambient conditions temperature range: 4 40 C; F air humidity below 90% humidity (non conden General Dimensions valve diameter 47.5 mm Weight 1.86 kg, without valve Variants valve drive valve head valve head material connection bore size max. flowrate 6-port none AWA10 2-position stainless steel / Vespel stainless steel / Vespel 1/16" 0.3 mm 50 ml/min AWA11CA 400 bar 1/16" 0.6 mm 100 ml/min AWA10AA 300 bar PEEK / PPS 1/16" 0.6 mm 50 ml/min AWA10AC 200 bar stainless steel / Vespel 1/8" 1.2 mm 500 ml/min AWA10AD 300 bar PEEK / PPS 1/8" 1.2 mm 500 ml/min AWA10AE 200 bar Ordering details: AWA11CA AWA10AA AWA10AC AWA10AD AWA10AE AZURA Valve Drive V 2.1S with 6-port 2-position valve, stainless steel, 1/16, 400 bar AZURA Valve Drive V 2.1S with 6-port 2-position valve, stainless steel, 1/16, 300 bar AZURA Valve Drive V 2.1S with 6-port 2-position valve, PEEK, 1/16, 150 bar AZURA Valve Drive V 2.1S with 6-port 2-position valve, stainless steel, 1/8, 300 bar AZURA Valve Drive V 2.1S with 6-port 2-position valve, PEEK, 1/8, 150 bar Order sample loops on page

34 Manual valves Manual injection and multi-position switching valve Switching and injection valves by KNAUER have impressive performance data and an outstanding long-term stability. The valve is equipped with a REED contact that is closed (magnetically controlled) during injection. The start signal can be easily started with an integrator or HPLC software. The sample loops are also suitable for partial filling due to the fact that they flow in the reverse direction when injected (filled). In turn, this aids in the minimization of peak broadening. Specifications Manual Valves Valves 2-position Valve type manual Valve head material stainless steel / PEEK Rotor seal material Vespel /PPS Capillary connection 1/16" / 1/8 Ambient conditions temperature range: 4 40 C; F air humidity below 90% humidity (non-condensing) General Dimensions valve diameter 47.5 mm Weight 0.36 kg Order mounting brackets on page 47. valve head valve head material connection pressure bore size max. flowrate article number 6 port 2-position stainless steel / Vespel stainless steel / Vespel 1/16" 400 bar 0.3 mm 50 ml/min AVI26BC 1/16" 300 bar 0.6 mm 100 ml/min A1357 PEEK / PPS 1/16" 200 bar 0.6 mm 50 ml/min A1358V1 stainless steel / Vespel 1/8" 300 bar 1.2 mm 500 ml/min A1359 PEEK / PPS 1/8" 200 bar 1.2 mm 500 ml/min A1360V1 Ordering details: AVI26BC A1357 A1358V1 A1359 A1360V1 Manual 6-port 2-position valve, stainless steel, 1/16", 400 bar Manual 6-port 2-position valve, stainless steel, 1/16", 300 bar Manual 6-port 2-position valve, PEEK, 1/16", 200 bar Manual 6-port 2-position valve, stainless steel, 1/8", 300 bar Manual 6-port 2-position valve, PEEK, 1/8", 200 bar Order sample loops on page

35 Ultra high pressure valves 2-position and multi-position valve KNAUER s well-known high pressure and ultra-high pressure pumps and detectors based on AZURA technology are now accompanied by the next generation of high precision injection and switching valves, designed to operate at pressures up to 1200 bar. This new valve series is ideal for users requiring low delay volumes and high durability and high performance. Specification valves Valves 2 position valve / multiposition valve Valve type manual & for valve drive (VICI valve drive, not compatible to V 2.1S) Valve head material diamond coated stainless steel Maximum pressure stability 1200 bar Bore Size 0.3 mm Rotor seal material Vespel Capillary connection 1/16" Ambient conditions temperature range: 4 40 C; F air humidity below 90% humidity (non-condensing) Please note that for valves without manual lever, the VICI valve drive has to be ordered separately. Order mounting brackets on page 47. valve valve head material connection pressure bore size article number Manual 6 port 2-position DLC stainless steel / 1/16" 1200 bar 0.3 mm AVI28AC Manual 8 port 2-position Vespel AVI38AC 6 port 2-position AVC28AC 8 port 2-position AVC38AC 6 port multiposition AVR28AC 8 port multiposition AVR38AC Ordering details: AVI28AC AVI38AC AVC28AC AVC38AC AVR28AC AVR38AC AWB02 Manual 6 port 2-position valve, DLC stainless steel, 1/16, 1200 bar Manual 8 port 2-position valve, DLC stainless steel, 1/16, 1200 bar 6 port 2-position valve w/o drive, DLC stainless steel, 1/16, 1200 bar 8 port 2-position valve w/o drive, DLC stainless steel, 1/16, 1200 bar 6 port multiposition valve w/o drive, DLC stainless steel, 1/16, 1200 bar 8 port multiposition valve w/o drive, DLC stainless steel, 1/16, 1200 bar VICI valve drive Order sample loops on page

36 Online degasser AZURA Degasser DG 2.1S Dissolved gases in the solvent can cause bubbles in the flow path of pumps and detectors. Reliable chromatographic separation therefore requires degassing of the solvent. The small analytical 2-channel degasser AZURA Degasser DG 2.1S is equipped with two degassing chambers and can thus degas two solvents simultaneously. Key features Degassing of up to two eluents simultaneously Degasser nominal performance: 2ml/min per channel Small dead volume Needs very little bench space Specifications Degasser Module Degasser channels 2 Degasser maximum flow rate 10 ml/min Degassing method gas permeation through a fluoropolymer membrane Dead volume Solvent applicability Pressure decline Maximum pressure stability Wetted materials Display approx. 285 µl per channel universal, except hydrochloric acid and halogenated hydrocarbons especially hexafluoro isopropanol (HFIP), hexane, heptane 1.37 mm (Hg /ml /min) 7 bar Systec AF, PEEK, glass-filled PTFE 1 LED General Dimensions 121 x 138 x 190 mm (W x H x D) Weight 2.3 kg Power supply V, Hz, 20 W Ambient conditions temperature range: 4-40 C, F air humidity: below 90%, non-condensing Feature overview for standalone devices Type degasser channels degasser maximum flow rate degassing chamber volume vacuum pump AZE ml/min 285 µ per channel 1.37 mm (Hg /ml /min) A ml/min 480 µl per chamber 1.37 mm (Hg /ml /min) A ml/min 7.7 ml per chamber > 6.2 kpa : 400 cycles/min < 6.2 kpa : 60 cycles/min Ordering details: AZE02 A5335 A5328 AZURA Degasser 2.1S analytical, 2 channels 2-channel online GPC degasser, analytical, stand alone 480 µl dead volume Hexane compatible 2 channel online degasser, semi-preparative 30

37 Osmometer K-7400S Semi-Micro Osmometer Freezing Point Osmometer KNAUER is one of the pioneers in the field of osmometry and known for its reliable and user friendly instruments for many decades. Our newest freezing point Osmometer K-7400S allows the easy and fast determination of the osmolality of various aqueous solutions. In addition, the freezing point depression of such samples can be measured. The proven technology of freezing point determination in combination with the robust and intelligent design of the device allows fast and reproducible measurements. The instrument is equipped with a peltier cooler and an integrated microprocessor controlling the automated measurement. The freezing point Osmometer is a standalone device that optionally can be equipped with a printer. Furthermore, the device can be controlled via the EuroOsmo 7400 software. The software automatically plots the temperature curve for each measurement and calibration and allows saving of the measured values. In addition, the data can optionally be exported into various file formats for archival storage. Key features High precision measuring head - Made in Germany Variable sample volume between 50 and 150 µl Software EuroOsmo 7400 for control and data management Easy and fast measurement of solutes Small, quite and robust - Perfect for your lab Specifications Sample volume µl Osmolality range mosmol/kg Resolution 1 mosmol/kg Test time ~ 2 min Precision SD 4 mosmol/kg [0-400 mosmol/kg] RSD 1 % [ mosmol/kg] Linearity ± 1 % [ mosmol/kg] ± 1.5 % [ mosmol/kg] Calibration Two-point Calibration (0 mosmol/kg and one free selectable osmolality) optional: Three-point Calibration (0 Osmol/kg and two free selectable osmolalities) Communication Interfaces RS-232 port Control Keypad (LED display, 2 rows with 24 characters) optional: EuroOsmo7400 Software General Dimensions 160 x 182 x 340 mm (W x H x D) Weight 4.5 kg Power supply V, Hz, 70 W Ambient conditions temperature range: C air humidity: %, non-condensing Ordering details: A0006AC K-7400S Semi-Micro Osmometer A Measuring head for plastic sample tubes A3705 EuroOsmo 7400, control software for osmometer K-7400S A3711 Plain paper printer, with power supply 31

38 Software Mobile Control (Chrom) for Windows 10 The Mobile Control (Chrom) is an intuitive, simple and cost-effective software solution for controlling and monitoring your KNAUER devices and systems. The friendly system overview provides instant insight into device and system status and allows you to adjust key parameters with your fingertips. The smart user management makes the Mobile Control safe; ensuring that device parameters only be changed by authorized operators. The graphical display of GLP data helps you avoid problems and unexpected downtime as well as to plan maintenance controls. With the hand-held Mobile Control option you can check your system remotely, allowing you to oversee multiple systems from the comfort of your office. Alternatively you can install the Mobile Control directly on your PC. Free updates enable you to take advantage of new devices. Your Mobile Control license can be easily upgraded to Mobile Control Chrom with data acquisition and analysis. For a free demo version, please visit the KNAUER website: System requirements Operating system: Windows 10 Communication: WIFI or LAN Display size: minimum 10" Display resolution: 1024 x 768 or 1280 x 800 (optimized) RAM: 1 GB (Mobile Control), 2 GB (Mobile Control Chrom) Processor: 1.5 GHz, Dual-Core Disc space: 200 MB Supported devices on page 69. Ordering details: A9607 A9608 A9610 A9612 A9614 A9617 A96181 A64809 A64809INT A64811 Mobile Control without data acquisition, including 10 tablet, power supply and tablet mount Mobile Control Chrom with data acquisition, including 10 tablet, power supply and tablet mount Mobile Control license without data acquisition Mobile Control Chrom license with data acquisition Upgrade license Mobile Control to Mobile Control Chrom Mobile Control Mount flexible tablet mount for 7-10 tablets USB-LAN ADAPTER Network adapter USB 2.0 <-> 10/100 Ethernet for tablets WLAN Router, 8-port Gigabit RJ-45 WLAN Router with international power supply wo plug, 8-port Gigabit RJ-45 Single device WLAN router for Mobile Control 1x RJ45, 10/100 MBit, WLAN, WLAN router for single device 32

39 Software ClarityChrom ClarityChrom is an easy-to-use Chromatography Data System for workstations. The optional extensions for GC control, PDA and GPC allow to use the software for a wide spectrum of application. Along to all KNAUER devices, devices and systems from more than 45 manufacturers are supported. The ClarityChrom Prep includes the drivers for several fraction collectors and supports the peak recognition by level and/or slope. The manual fraction control and the option to use the KNAUER electric valves for fractionation gives you more flexibility. Due to the abundance of supported devices ClarityChrom allows to update installed systems at a later state and even to operate in system hardware of different manufacturer. The software is in many language version available such as German, Russian, Chinese, French, Spanish, etc. Specifications Package ClarityChrom System architecture 32-bit CDS Operating system Windows 10, Windows 7, Windows 8/8.1, Windows Vista SP2, all 32- and 64-bit; Windows XP SP3 (32-bit only) Stand-alone workstation version, max. 4 systems controlled by one computer, max. 3 complete systems recommended Client/server no client/server functionality Multi-user environment selectable system of user accounts with independently customizable behavior and appearance for individual users Network environment easy offline data sharing (at the file level) among all stations in a local network Fields of application analytical and preparative HPLC, GPC/SEC, GC, MS Instrument connection supports RS-232, Ethernet, PCI interface card, A/D-D/A interface Recommended PC hardware Pentium 2 GHz, 2 GB RAM, 4 GB RAM for DAD with high data rate, 20 GB free hard disk space, separate graphics card if one PC should control >1 system Graphics capabilities multiple chromatogram view and overlay, PDA view Integration 27 integration parameters (peak width, threshold, tangent slope ratio etc.) integration parameters programmable in time, automatic re-integration Calculation types with/without calibration (int./ext. standard method) Security and GLP installation qualification test of the software; FDA 21 CFR Part 11 conformance, validation with virtual detector Instrument control method-based instrument control, Instrument status display and direct control mode, Calibration 6 types of calibration curves, up to 20 levels, reference peaks, groups, unlimited number of standards (peaks), LOD, LOQ Chromatogram overlay view, custom labels and settings, also applying mathematical operations to chromatograms operations Automation sequences, automatic launch of selected commands or applications immediately following chromatogram acquisition post run, batch Presentation of results Integrated customizable table of results, columns with userdefined calculation, summary table, and export in text or database format Calculations custom: 12 predefined mathematical operators, 15 basic and 4 summary functions, special: Kovats indexes for GC, determination of noise/drift, performance calculations Data import & export ASCII, AIA, dbase PDA option 3D chromatogram, peak purity analysis, spectrum search in self-made or commercial spectra library (separate license option) GPC/SEC option molecular weight determination in size exclusion chromatography with various calibration methods (separate license option) System suitability test automates the calculation of system suitability parameters for system validation (separate license option) Supported devices on page 61. Ordering details: A1670* ClarityChrom single instrument license for one time base A1674* ClarityChrom offline license for data evaluation A1671* ClarityChrom additional instrument license on additional time base A1676* ClarityChrom option for PDA data processing A1681* Upgrade for one system from former version to ClarityChrom or ClarityChrom Prep A1685* ClarityChrom Prep single instrument license for one time base A1688* ClarityChrom Prep offline license for data evaluation A1686* ClarityChrom Prep additional instrument license on additional time base A1677* System suitability option A1678* Option for GPC data processing A1679* Option for MS data processing A1690 ClarityChrom Prep Trial with 30 day Trial license dongle must send back after trial period A1675* ClarityChrom university package one offline license * a sequential number is added to the article number which depends on the software version. At the time of printing, the current sequential number is -11 (e.g. A ) for ClarityChrom Version

40 Software PurityChrom PurityChrom is a chromatography software especially designed for purification and FPLC applications. PurityChrom provides a very user friendly and clearly structured interface. The system visualization provides a graphical representation and easy handling of the complex flow processes. Furthermore, each device which is displayed in the fluidic scheme can be controlled manually. This provides the opportunity to change and adapt your conditions spontaneously during the run. You have the option to create methods based on volume, column volume or time. The software allows you an exclusive monitoring of threshold values. This enables numerous programming possibilities, like peak fractionation, pressure monitoring and air bubble detection. The data analysis is intuitive and allows you to integrate peaks manually. Solvent visualization calculates the consumption of solvent for the current run and prevents your column from running dry. For fractionation, you can use a fractionation valve as well as a fraction collector. Offline licenses are available for free on the KNAUER website: Specifications PurityChrom Package PurityChrom Basic License: 8 devices (one modul counts except detectors) 3 data channels autosampler not supported Full license: no limitation in device number 8 data channels autosampler supported DAD license: 3D option Operating system Windows XP, Windows Vista, Windows 7, Windows 8, Windows 10 Stand-alone Fields of application for one system and one computer FPLC and Prep LC Supported instruments all KNAUER AZURA devices, the Foxy R1/R2 Fraction Collector (see Release Notes) and many other devices, detailed information on request Instrument connection Recommended PC hardware Integration Security and GLP Automation Presentation of results Calculations supports RS-232, Ethernet, A/D-D/A interface CPU/Memory: Pentium III or higher with at least 1 MHz, 32 or 64 Bit at least 512 MB RAM (Windows XP) and 2 GB (Windows Vista and higher) graphics: Screen with minimal resolution 1024 x 768 connectors and Slots: USB for license dongle, COM, USB or LAN according to connected instruments real-time analysis of peaks, automatic or manual integration and baseline correction FDA 21 CFR Part 11 conformance sequences individual report configuration column performance calculations according to DAB Data import and export comma Separated Value, AIA/ANDI, ChromStar Slice Special features FRC option method creation based on volume or column volume, hold function, functionalised visualisation, direct control during a run, display of solvent supply, waste management included Supported devices on page 61. PCs on page 37. Ordering details: A2650 A2652 A2654 PurityChrom Basic License PurityChrom Upgrade to full version inclusive autosampler option PurityChrom DAD License, for full version 34

41 Software OpenLAB CDS EZChrom Edition OpenLAB CDS EZChrom Edition is the next generation of chromatography data systems and the successor of ChromGate CDS. OpenLAB CDS EZChrom Edition provides chromatography data acquisition, processing and control of GC and LC chromatographs and is used in chromatography operations ranging from single user/single instrument to multi-user/multi-instrument laboratories. It provides support of devices from KNAUER and many other manufacturers. The Shared Services module is the base of the OpenLAB CDS architecture. The OpenLAB Shared Services Control Panel allows for all administrative activities, as system and interface configuration, administration of users and built-in administrative reports, in a uniform interface. The OpenLAB CDS EZChrom Edition offers advanced functionality with an interface known from the ChromGate CDS. If you already work with ChromGate or EZChrom Elite, you will find the OpenLAB CDS EZChrom Edition as a simple upgrade regarding usability and workflow. The basic workstation license can only be installed on one PC and allows for control and data acquisition from one system. The license includes System Suitability, Fraction Collector Control and one year Software Maintenance Agreement (SMA). OpenLAB and EZChrom Elite are registered trademarks of Agilent Technologies, Inc. Specifications Package System architecture OpenLAB CDS EZChrom Edition 32-bit CDS Operating system workstation or client (A.04.06): Windows 7 Professional / Enterprise 32-bit and 64-bit, Windows 8.1 Enterprise or Professional 32-bit and 64-bit; Shared Services Server: Windows Server 2008 R2 Stand-alone on a workstation max. 3 systems controlled by computer recommended Client/server Multi-user environment Network environment Fields of application Instrument connection Recommended PC hardware Graphics capabilities Integration Calculation types Security and GLP Instrument control Calibration Chromatogram operations Automation Presentation of results Calculations Data import and export Special features FRC option FRC features PDA option GPC/SEC option System suitability test client/server functionality allows for using license keys from any computer in the network, full control of a control-pc (server) from any other computer (Client) in the network (Distributed System) allows to create users in the system with individual access management or to apply users from the Windows domain or local PC runs on Windows networks based on TCP/IP analytical HPLC, preparative HPLC, GPC/SEC, GC supports RS-232, Ethernet, A/D-D/A interface workstation or client: Pentium 3 GHz, 4 GB RAM, 160 GB free hard disk space, separate graphics card if one PC should control more than one system; Shared Services Server: Pentium 3 GHz, 4/6 GB RAM (32/64 bit), 160 GB free hard disk space multiple chromatogram view and overlay, PDA view, arrange all open windows real-time analysis of peaks, automatic or manual integration and baseline correction with/without calibration (int./ext. standard method) installation qualification test (IQ) of the software; FDA 21 CFR Part 11 conformance, validation of integration method-based instrument control, Instrument status display and Direct-Control mode, Direct Control can also be enabled for a run several types of calibration curves, unlimited number of levels, several reference and ISTD peaks possible, groups, unlimited number of standards (peaks), LOD, LOQ overlay view, mathematical operations and smoothing sequences, Run Queue allows submitting of single runs and/or sequences, automatic launch of selected commands, user programs or applications if the data acqusition is finished, post run, batch different report templates available, method custom report and advanced reports for individual reports, new Intelligent Reporter allows for creating complex reports in a drag-and-drop editor with pre-configured tables, graphics and matrices, results can be shown in chromatogram mathematical operations and smoothing, column performance calculations USP, DAB ASCII, ANDI (AIA) standard, OLE, ODBC Direct Control during a run, pretreatment, stacked injection always included, for preparative HPLC, adds tools for detector controlled fraction collection, solvent and peak recycling fractionation can be controlled by time (volume), level, slope including AND/OR combination of these criteria, spectra comparison, local maximum and local minimum, slices, full manual control of fractionation during a run 3D chromatogram, peak purity analysis, spectrum search in self-made or commercial spectra library (separate license option) molecular weight determination in size exclusion chromatography with various calibration methods (separate license option) always included, automates the calculation of system suitability parameters for system validation 35

42 Software OpenLAB CDS EZChrom Edition Supported devices on page 61. PCs on page 37. Ordering details: A OpenLAB CDS EZChrom Edition workstation for one system with SMA and 4x System Suitability A OpenLAB CDS EZChrom Edition 3D option for UV detectors MW-1, 2550 and 2600 A OpenLAB CDS EZChrom Edition 3D UV Option for DAD DAD6.1L, DAD2.1L, PDA-1, S2850 A OpenLAB CDS EZChrom Edition drivers Sedex 80/85/90LT/LC not for fraction collection A OpenLAB CDS EZChrom Edition SEC/GPC option A OpenLAB CDS EZChrom Edition Workstation, Upgrade from ChromGate Standalone with existing options A OpenLAB CDS EZChrom Edition Workstation license Right-to-copy, one additional system with SMA and 4x System A OpenLAB CDS EZChrom Edition Instrument Control License A OpenLAB CDS EZChrom Edition for distributed systems - please ask for desired configuration Thermo Scientific Dionex Chromeleon chromatography data system driver Thermo Scientific Dionex Chromeleon is one of the most wide-spread chromatography data systems. Its intuitive handling benefits laboratory workflow and the highly developed algorithms simplify data processing. It offers a broad range of third-party drivers and can be easily used with existing HPLC systems. KNAUER offers drivers for a lot of its devices. Disclaimer: KNAUER Wissenschaftliche Geräte GmbH is solely responsible for development, testing and support of Thermo Scientific Dionex Chromeleon Chromatography Data System driver software for KNAUER instruments and therefore solely liable for damages associated with the use of this driver software. Supported devices on page 61. PCs on page 37. Computer requirements Operating system CPU (recommended) Memory RAM (recommended) Free Hard Disk Space Optical Drive Display (recommended) USB Ports Ethernet Port Windows 8.1 Professional, 64-bit;Windows 7 SP1 Professional, Enterprise, 64-bit, (32-bit version is not recommended); Windows Vista SP2 Business, Ultimate, 32-bit (Vista is not recommended) 3 GHz Intel Core i7 or better 8 GB 120 GB available, for system with PDA detectors DVD 1280 x 1024, 32-bit color 1 port for USB license key 1 port for router (for system connection) Ordering details: A1780* Thermo Scientific Dionex Chromeleon Bundle Workstation Open. Complete Software Package incl. License Dongle A1782* Thermo Scientific Dionex Chromeleon Instrument Controller Option Instrument Class 3. Max 2 LC per workstation A1783* Thermo Scientific Dionex Chromeleon Driver CD A1784* Thermo Scientific Dionex Chromeleon Instrument Controller Option - 3D Data Acquisition * a sequential number is added to the article number which depends on the software version. At the time of printing, the current sequential number is -2 (e.g. A1783-2) for Thermo Scientific Dionex Chromeleon Version

43 PC hardware & IT accessories / power connections PC hardware & IT accessories CDS Workstation A13110 Chromatography Workstation for PurityChrom and ClarityChrom with 24" monitor, Windows 10, Intel Core i5, 8 GB RAM, 256 SSD, two network cards, german edition A13111 Chromatography Workstation for OpenLAB and Chromeleon with 24" monitor, Windows 7, Intel Core i7, 8 GB RAM, 256 SSD, two network cards, german edition A13112 Laptop for PurityChrom and ClarityChrom, Windows 10, Intel Core i3, 8 GB RAM, 256 SSD, german edition A13113 Laptop for OpenLAB and Chromeleon, Windows 7, Intel Core i5, 8 GB RAM, 500 GB HDD, german edition A13114 A13120 A13121 Microsoft Surface Pro 4 for PurityChrom, Windows 10, Core m3, 4GB RAM, 128 GB SSD, german edition with keyboard, Surface Pen and docking station Chromatography Workstation for PurityChrom and ClarityChrom with 24" monitor, Windows 10, Intel Core i5, 8 GB RAM, 256 SSD, two network cards, international edition Chromatography Workstation for OpenLAB and Chromeleon with 24" monitor, Windows 7, Intel Core i7, 8 GB RAM, 256 SSD, two network cards, international edition Configuration on request A13130 PC, special edition on request Network devices A64809 A64809INT A3119 A3119INT A3126 A3126INT WLAN Router, 8-port Gigabit RJ-45 WLAN Router with international power supply wo plug, 8-port Gigabit RJ-45 8-port LAN GB Switch NetGear GS108GE 8x RJ-45, GBit, Auto-MDIX 8-port LAN GB Switch NetGear GS108GE 8x RJ-45, Gigabit, Auto-MDIX power plug UK/US/ AUS 5-port Fast Ethernet Switch NetGear FS105 5x RJ-45, 100 MBit 5-port Fast Ethernet Switch NetGear FS105 5x RJ-45, 100 MBit power plug UK/US/AUS IT accessories A3114 VSCOM USB 4 COM 4 x RS-232 DE9 on USB AZB00XA AZURA Interface Box IFU 2.1 LAN, A/D converter, 4 channels M0447V1 UK plug for WLAN router / Switch M0447V2 US plug for WLAN router / Switch M0447V3 AUS plug for WLAN router / Switch A5255 Patch cable CAT5e RJ45/RJ45 3m A0895 RS232 f/f cable 9-pol nullmodem A0884 RS232 m/f cable 9-pol A3121 APC Smart UPS 1500 VA, uninterruptible power supply Power cables M1642 Power cable for Europe, 2 m, with rubber connector type C13, 230 V M1597 Power cable for Switzerland, 2 m, with rubber connector type C13, 230 V M1278 Power cable for UK, 2.5 m, with rubber connector type C13, 230 V M1651 Power cable for USA, 2 m, with rubber connector type C13, 115 V M2651 Power cable, 1.5 m, with rubber connector for UPS APC Smart connector AZS80SA Distribution Box 24 V for 6 devices like Air sensor, external pressure sensor, IFU 2.1 LAN 37

44 Column kits Detailed information available via KNAUER website: RP column kit BRP_KIT01 BRP_KIT02 BRP_KIT03 BRP_KIT08 BRP_KIT09 BRP_KIT12 BRP_KIT15 RP analytical Column Kit 5 µm, Eurospher II, 3 columns (C18, C18A, C8) 150 x 4 mm with integrated precolumn RP analytical Column Kit 3 µm, Eurospher II, 3 columns (C18, C18A, C8) 100 x 3 mm with integrated precolumn RP UHPLC Column Kit 2 µm, Eurospher II, 3 columns (C18, C18A, C8) 50 x 2 mm RP analytical Column Kit 3 µm, Eurospher II, 3 columns (C18H, C8, C18A) 150 x 4 mm ID RP analytical Column Kit 3 µm, Eurospher II, 3 columns (C18, C18P, C18A) 150 x 3 mm ID RP UHPLC Column Kit 2 µm, Eurospher II, 3 columns (C18, C8, C18A) 100 x 2 mm ID RP analytical column Kit 5 µm, Eurospher II, 3 columns (C8, C18, C18A) 250 x 4 mm ID with integrated precolumn NP column kit BNP_KIT01 BNP_KIT02 NP analytical Column Kit 5 µm, Eurospher II (Si, NH2, Diol) 150 x 4 mm with integrated precolumn NP analytical Column Kit 3 µm, Eurospher II (Si, NH2, Diol) 100 x 3 mm with integrated precolumn Mixed column kit BMIX_KIT01 BMIX_KIT02 Mixed analytical Column Kit 5 µm, Eurospher II (C18, C8, NH2), 150 x 4 mm ID Mixed analytical Column Kit 5 µm, Eurospher II (C18H, Phenyl, Si), 150 x 4.6 mm ID GPC column kit BGPC_KIT01 BGPC_KIT02 BGPC_KIT03 BGPC_KIT04 GPC column kit in THF, low MW: kda, PSS SDV (3 x 1,000 A, 3 µm) 300 x 8 mm with precolumn 50 x 8 mm GPC column kit in THF, medium MW: 0.1-1,000 kda, PSS SDV (1,000 A, 100,000 A, 5 µm) 300 x 8 mm with precolumn 50 x 8 mm GPC column kit in THF, high MW: 0.1-3,000 kda, PSS SDV (1,000 A, 100,000 A, 1,000,000 A, 5 µm) 300 x 8 mm with precolumn 50 x 8 mm GPC column kit in THF, ultrahigh MW: ,000 kda, PSS SDV (1,000 A, 100,000 A, 10,000,000 A, 10 µm) 300 x 8 mm with precolumn 50 x 8 mm SEC column kit BSEC_KIT01 BSEC_KIT02 BSEC_KIT03 SEC column kit aqueous, low MW: kda, PSS SUPREMA (3 x 1,000 A, 5 µm) 300 x 8 mm with precolumn 50 x 8 mm SEC column kit aqueous, medium MW: 0.1-1,000 kda, PSS SUPREMA (30 A, 2 x 1,000 A, 5 µm) 300 x 8 mm with precolumn 50 x 8 mm SEC column kit aqueous, high MW: 0.1-3,000 kda, PSS SUPREMA (100 A, 2 x 3,000 A, 10 µm) 300 x 8 mm with precolumn 50 x 8 mm BSEC_KIT04 SEC column kit aqueous, ultrahigh MW: ,000 kda, PSS SUPREMA (3 x Ultrahigh, 10 µm) 300 x 8 mm with precolumn 50 x 8 mm 38

45 Pump accessories Eluent tray and bottles AZC00 AZURA Eluent tray E 2.1L for AZURA devices with a capacity of 6 x 1 l bottles or 4 x 2.5 l bottles or 2 x 5 l bottles, (delivery without bottles) A5325 A2056 A5324 Eluent supply bottle, 1 L bottle (borosilicate glass) with special round bottom for minimal eluent remainder, includes screw-type cap 250 ml Bottle for Piston Back Flushing Set of eluent supply bottles 4 x 1 L bottles (borosilicate glass) with special round bottom for minimal eluent remainder, includes screw-type cap A Set of eluent supply bottles, 2 x 1 L bottles (borosilicate glass) with special round bottom for minimal eluent remainder, includes screw-type cap A Set of eluent supply bottles, 4 x 1 L bottles (borosilicate glass) for Eluents and 1 x 250 ml bottle piston backflushing A70037 Set of eluent supply bottles, 3 x 2.5 L brown glass bottles (borosilicate glass) with special round bottom for minimal eluent remainder, for preparative HPLC/FPLC, includes screw-type cap A70037 AZC00 A5324 A A A2056 Solvent filters and pump inlet tubing A3373 A3374 A3364 A9650 A96507 A9661 A96601 Mobile Phase Filter, stainless steel, 2 µm, 1/8" pipe OD, suitable for all analytical HPLC systems Mobile Phase Filter, stainless steel, 20 µm, 1/8" pipe OD, suitable for all analytical and semi preparative HPLC systems, max. flow rate 100 ml/min Mobile Phase Filter, Biocompatible PE, 20 µm, 1/8 pipe OD, suitable for all FPLC systems, max. flow rate 500 ml/min AZURA Tubing Kit with cap and inset, solvent filter inlet and fittings, 1 set AZURA Tubing Kit bio with cap and inset, solvent filter inlet and fittings, 1set Filter Cartridge for pump P 6.1L, Titanium frit, 2 µm pore size. 50 ml/min maximum flow, High capacity filter, 60 µl volume, 3 pcs. Filter Cartridge for pump P 6.1L, Stainless steel frit, 2 µm pore size. 10 ml/min maximum flow, Volume optimized filter, 20 µl volume, 3 pcs A3373 A3374 A3364 A96507 A9650 Static mixers AZZ00MB AZURA HPLC mixer up to 100 MPa, 50 µl mixing volume AZZ00MC AZURA HPLC mixer up to 100 MPa, 100 µl mixing volume AZZ00MD AZURA HPLC mixer up to 100 MPa, 200 µl mixing volume AZZ10ME AZURA HPLC mixer up to 40 MPa, 250 µl mixing volume (biocompatible) A5830 HyperShear Static Mixer, (1.5mL), high flow series, SST A Mounting bracket AZURA L for Hypershear mixing chambers AZZ00MB & AZZ00MC A5830 Dynamic mixers A0285 Dynamic mixing chamber, stainless steel, analytical, 1/16, up to 420 bar (42 MPa), 1740 µl mixing volume A0581 Dynamic mixing chamber, stainless steel, preparative, 1/8, up to 250 bar (25 MPa), 5.9 ml mixing volume A0275 Dynamic mixing chamber, titanium, analytical, 1/16, up to 420 bar (42 MPa), 1740 µl mixing volume A70581 Dynamic mixing chamber, titanium, preparative, 1/8, up to 250 bar (25 MPa), 5.9 ml mixing volume A2515 Mixing chamber extension unit, 1 intermediate section, titanium, 4.8 ml, 3 screws 6 x 60 mm AZZ10ME A

46 Pump accessories Pump head inlet fittings A9861 Pump head inlet for Pump AZURA P 2.1L, 80P 3/8" (NPT), stainless steel A9868 A98611 A98612 A98613 A58267 A58268 A58269 Pump head inlet AZURA for Pump P 2.1L, Set, 1/2"-20 UNF, PEEK with CTFE (Kel-F) adapter, including tubing 1/4 PTFE Bushing inlet for AZURA Pump P2.1L BlueShadow 80P adapter to 3/8" tube stub Bushing inlet for AZURA Pump P2.1L BlueShadow 80P LPG binary to 3/8" tube stub Bushing inlet for AZURA Pump P2.1L BlueShadow 80P LPG ternary to 3/8" tube stub Male Connector, stainless steel, 1/4"Tube OD x 1/4" Male NPT Male Connector, stainless steel, 4 mm Tube OD x 1/4" Male NPT Male Connector, stainless steel, 1/8" Tube OD x 1/4" Male NPT A58202 Inlet-bushing kit 1/8" capillary, 10 ml pump heads (P 2.1S, P 4.1S, S1050, P 6.1L, 40P) A58203 Inlet-bushing kit 1/16" capillary, 10 ml pump heads (P 2.1S, P 4.1S, S1050, P 6.1L, 40P) A58204 Inlet-bushing kit 1/8" capillary, 10 (ceramic) and 50 ml (all) pump heads (P 2.1S, P 4.1S, S1050, P 6.1L, 40P) A58205 Inlet-bushing kit 1/16" capillary, 10 (ceramic) and 50 ml (all) pump heads (P 2.1S, P 4.1S, S1050, P 6.1L, 40P) A58207 Tubing kit for pump head inlet AZURA for 1/4''-PTFE tubing, for pump P 2.1L, 80P, S1800, including solvent filter A9861 Pump head outlet fittings A5822 Outlet-bushing kit 1/8 pipe socket for pumps S1800, 80P and P 2.1L A7200 Adapter 1/8 external thread on 1/16 internal thread, 2 pieces Mass flow controllers* A5390 Mini CORI-Flow (M13) Mass flow controller incl. mounting block, capacity: ml/min A5391 Mini CORI-Flow (M14) Mass flow controller incl. mounting block, capacity: ml/min A5393 Mini CORI-Flow (M13) Mass flow controller incl. mounting block, capacity: ml/min, Profibus A5394 Mini CORI-Flow (M12) Mass flow controller incl. mounting block, capacity: 0,03-3,3 ml/min A5395 Mini CORI-Flow (M13) Mass flow controller incl. mounting block, capacity: ml/min, Hastelloy A5396 HI-TEC Bright display for Mini CORI-Flow mass flow controller (display, setpoint and counter) *analog and bus versions on request A

47 Pump accessories Replacement pump head AZURA Pump P 4.1S & P 2.1S AHB40 AHB32 AHB32DA AHB43 AHC20 AHC22 AHC23 Pump head 10 ml, stainless steel Pump head 10 ml, ceramic Pump head 10 ml, ceramic with Ti-bushings Pump head 10 ml, Hastelloy-C, for corrosive chemicals Pump head 50 ml, stainless steel Pump head 50 ml, ceramic Pump head 50 ml, Hastelloy-C, for corrosive chemicals A Replacement pump head AZURA Pump P 6.1L AHA60 AHB40 AHB40BA AHB40CA AHB32 AHB32DA AHC20 AHC20BA AHC22 AHC23 AHB43 Pump head 5 ml, stainless steel (1000 bar) Pump head 10 ml, stainless steel Pump head 10 ml, stainless steel, for normal phase applications Pump head 10 ml, stainless steel, for high temperature dosing applications Pump head 10 ml, ceramic Pump head 10 ml, ceramic with Ti-bushings Pump head 50 ml, stainless steel Pump head 50 ml, stainless steel, for normal phase applications Pump head 50 ml, ceramic Pump head 50 ml, Hastelloy-C, for corrosive chemicals Pump head 10 ml, Hastelloy-C, for corrosive chemicals Replacement pump head BlueShadow 40P AHB40 AHB32 AHC20 AHC22 AHB40CA AHC20CA Pump head 10 ml, stainless steel Pump head 10 ml, ceramic Pump head 50 ml, stainless steel Pump head 50 ml, ceramic Pump head 10 ml, stainless steel, for high temperature dosing applications Pump head 50 ml, stainless steel, for high temperature dosing applications AHB40CA Replacement pump head AZURA Pump P 2.1L / BlueShadow 80P A Pump head 100 ml, stainless steel A4029V1 Pump head 100 ml, titanium A Pump head 250 ml, stainless steel A4021V1 Pump head 250 ml, titanium A Pump head 500 ml, stainless steel A Pump head 1000 ml, stainless steel A4022V1 Pump head 1000 ml, titanium Check valves A06841 Check valve unit for 10 ml (AZURA Pump P 4.1S/ P 2.1S, BlueShadow 40P) A06842 Check valve unit for 50 ml (AZURA Pump P 4.1S/ P 2.1S, BlueShadow 40P) A A A A1122 A1080 Check valve unit for (KEL-F) 10 ml pump head (AZURA Pump P 4.1S/ P 2.1S, BlueShadow 40P) Check valve unit for (KEL-F) 50 ml pump head (AZURA Pump P 4.1S/ P 2.1S, BlueShadow 40P) Check valve unit (spring-loaded) for 10 ml and 50 ml pump head (AZURA Pump P 4.1S/ P 2.1S, BlueShadow 40P) Check valve unit for 100 ml and 250 ml pump head (AZURA Pump P 2.1L, BlueShadow 80P) Check valve unit for 500 ml and 1000 ml pump head (AZURA Pump P 2.1L, BlueShadow 80P) 41

48 Pump accessories / Autosampler accessories Maintenance kits A96423 A96424 A96425 A96426 A96427 A96428 A58211 Maintenance kit for AZURA Pump P 4.1S & P 2.1S, AZURA Pump P 6.1L, BlueShadow 40P, 10 ml pump head, including 1 set of gaskets, 2 piston rods, 2 sapphire backing rings, 2 O-rings Maintenance kit for AZURA Pump P 4.1S & P 2.1S, AZURA Pump P 6.1L, BlueShadow 40P, 50 ml pump head, including 1 set of gaskets, 2 piston rods, 2 sapphire backing rings, 2 O-rings Maintenance kit 100 ml for AZURA Pump P 2.1L and BlueShadow 80P, including 2 sets of gaskets, 2 piston rods, 2 springs Maintenance kit 250 ml for AZURA Pump P 2.1L and BlueShadow 80P, including 2 sets of gaskets, 2 piston rods, 2 springs Maintenance kit 500 ml for AZURA Pump P 2.1L and BlueShadow 80P, including 2 sets of gaskets, 2 piston rods, 2 springs Maintenance kit 1000 ml for AZURA Pump P 2.1L and BlueShadow 80P, including 2 sets of gaskets, 2 piston rods, 2 springs Rebuild-Kit for Pump AZURA Pump P 2.1L and BlueShadow 80P (100ml/250 ml), Venting screw KEL-F, Check valve unit KEL-F, O-ring A Rebuild-Kit for AZURA Pump P 4.1S & P 2.1S, 10 ml/min pump head., Venting screw KEL-F, Check valve unit KEL-F, O-ring (FFKM) A Rebuild-Kit for AZURA Pump P 4.1S & P 2.1S, 50 ml/min pump head., Venting screw KEL-F, Check valve unit KEL-F, O-ring (FFKM) Autosampler accessories Autosampler AS 6.1L A0638 Crimp neck vial set N11, clear, 1.5 ml, crimp caps aluminum, septa rubber/tef colorless, 1,000 pcs. each A0664 Accessory kit for autosampler with vials, caps, septum and pliers for opening and closing A1585 Vial kit for preparative auto sampler with 10 ml vials and PE caps (1000 pcs.) A Vial plate for 84x1.5ml and 3x10ml vials for 3950 autosampler A Prep vial plate for 12x 10ml for 3950 Autosampler A50050 Vial plates for 1.5 ml vials for 3950 Autosampler 2 pcs. A50077 Sample loop 100 µl, Stainless Steel for AZURA Autosampler 3950 A50078 Sample loop 10 µl, Stainless Steel for AZURA Autosampler 3950 A50079 Kit for large injection samples for AZURA Autosampler 3950, incl. injection syringe 1000 µl, sample loop 500 µl A64700 Sample needle kit for AZURA Autosampler 3950 and AS 6.1L for SPARK valve 1/16 M0355 Plunger replacement tip 250 ul M2644 Rotor seal (100MPa) for UHPLC valve 18KPSI M2868 Preventive Maintenance Kit A /A50053/A /AAA20AA/AAA21AA Alias 3950 and AS6.1L- BIO M2869 Preventive Maintenance Kit A50060/A500601/A50070/A500701/AAA10AA/AAA11AA Alias 3950 and AS 6.1L- 100MPa M3053 Alias 3950 and AS 6.1L rotor seal (70 MPa) M3054 Preventive Maintenance Kit A50080/A50081 /AAA00AA/AAA01AA Alias 3950 and AS6.1L - 70MPa 42

49 Detector accessories Flow cells 1/16 A4042 A4045 A4061V2 A4061XB AMB18 AMC19XA AMC38 AMD59XA A64150 A64151 A4069 A4095 Flow Cell UV, 3 mm, 2 µl, 1/16", stainless steel Flow Cell UV, 3 mm, 2 µl, 1/16", PEEK Flow cell UV, 10 mm 10 µl, 1/16", stainless steel, with heat exchanger optimized for S2550 Flow cell UV, 10 mm 10 µl, 1/16", stainless steel, with heat exchanger one sided inlet and outlet 3mm, 2µl, 1/16", 300bar PressureProof Flow cell cartridge for AZURA Detector DAD/MWD 10mm, 2µl, 1/16", 50bar LightGuide Flow cell cartridge for AZURA DAD/MWD 10mm, 10µl, 1/16", 300bar PressureProof Flow cell cartridge for AZURA DAD/MWD 50mm, 6µl, 1/16", 50bar High Sensitivity LightGuide Flow cell cartridge for AZURA DAD/MWD UHPLC flow cell UV, 10 mm, 2.4 µl, 1/16" fiber optics for PDA-1 UHPLC flow cell UV, 50 mm, 10 µl, 1/16" LWL for PDA-1 Flow cell UV, 0.5 mm 1/16", stainless steel Flow cell UV, 0,5 mm 1/16", PEEK A4061V2 AMC19XA Flow cells 1/8 A4066 A4067 Flow Cell UV, 2 mm, 1/8", stainless steel, changeable to 0.5/1.25 mm Flow Cell UV, 2 mm, 1/8", PEEK, changeable to 0.5/1.25 mm Flow cells 1/4 A4068 Flow Cell UV, 2 mm, 1/4", stainless steel, changeable to 0.5/1.25 mm, without fittings A Flow Cell UV, 2 mm, straight connections, 1/4", stainless steel, changeable to 0.5/1.25 mm, without fittings Flow cells 1/16 fiber optics A A4044 A4044HT AMKX8KIT A4047 A4074 A4089 A4096 Flow cell UV, 3 mm, 2 µl, 1/16", stainless steel, double optic fiber connector Flow cell UV, 3mm 2µl 1/16" SS High Temperature up to 85 C double optic fiber connector -special version- Fiber optics adapter kit for AZURA DAD/MWD Flow Cell UV, 3 mm, 2 µl, 1/16", PEEK double optic fiber connector Flow cell UV, 10 mm, 10 µl, 1/16", stainless steel, double optic fiber connector with heat exchanger Flow cell UV, 0.5 mm 1/16", stainless steel double optic fiber connector Flow cell UV, 0.5 mm 1/16", PEEK double optic fiber connector Flow cells 1/8 fiber optics A4078 A4079 Flow Cell UV, 2 mm, 1/8", stainless steel, changeable to 0.5/1.25 mm double optic fiber connector Flow Cell UV, 2 mm, 1/8", PEEK, changeable to 0.5/1.25 mm double optic fiber connector A4044 A4078 Nano flow cell A4104 Nano flow cell, 3 mm double optic fiber connector, fused silica, 375 µm OD capillary connection A

50 Detector accessories Flow cells >1/8 fiber optics A4081 A4153 A4152 Flow Cell UV, 2 mm, 1/4", stainless steel, changeable to 0.5/1.25 mm, double optic fiber connector Special FlowCell, TriClamp1/4" 2mm, stainless steel 0.5/1.25/2 mm, fiber optical connector Special FlowCell, TriClamp3/8" 2mm, stainless steel 0.5/1.25/2 mmm, fiber optical connector A Special FlowCell, TriClamp3/8" 2mm, stainless steel 0.5/1.25/2 mmm, fiber optical connector A4154 A4155 Special FlowCell, TriClamp1/2" 2mm, stainless steel 0.5/1.25/2 mm, fiber optical connector Special FlowCell, TriClamp3/4" 2mm, stainless steel 0.5/1.25/2 mmm, fiber optical connector A4152 Fiber optic cables A0740 Fiber optic cables (2 pc), 750 mm, 2x SMA /660, polymicro A0743 Fiber optic cables (2 pc), customer made sizes, polymicro SMA /660 A0740HT Fiber Optical Connectors (2pc) 750 mm, up to 85 C Polymicro, FSMA 600/660 H+S Waste tubing kits A9842 Waste tubing kit LightGuide flow cells 1/16" A9843 Waste tubing kit UV flow cells 1/16" A9844 Waste tubing kit UV flow cells 1/8" A9843 Lamps A4071 Deuterium lamp, replacement, 35 W, original spare part A4447V1 Deuterium lamp, 35 W, for PDA K-2800, S-2800, S-2850 AZL01 Deuterium lamp type HBST for AZURA DAD 6.1L A64210 A5193 A5194 HBST deuterium lamp kit for MW-1 and PDA-1 E²PROM Connector Deuterium lamp, replacement, for S2550, S2520, 10D 40D, 50D, UVD 2.1S, UVD 2.1L DAD 2.1L, MWD 2.1L Deuterium lamp type HBST for S2550 (-XA) and 50D (-XA) AZL02 Halogen replacement lamp for AZURA DAD 6.1L A4073 A4073XA A64200 A64201 A4072 A4072XA Halogen lamp with base Halogen lamp with base Halogen lamp kit for MW-1 E²PROM Connector SND Halogen lamp kit for PDA-1 E²PROM Connector SND Tungsten-halogen lamp adjusted Tungsten-halogen lamp adjusted A4448 Tungsten-halogen lamp 7.5 W, with connector for TIDAS II adjusted, for PDA 2800 and PDA 2850 A5195 A5197 Tungsten halogen lamp, replacement 2550 and 50D for 2-lamp version, original spare part Tungsten halogen lamp, replacement 2550 for D2 lamp housing, original spare part A07541 LED for Sedex 80 and 85 A4142 A0753 A59210 Mercury vapor lamp with lamp bord, for WellChrom Fixed Wavelength Detector K-200 Xenon lamp RF 10 AXL, 150 W Xenon lamp for RF-20A/Axs 44

51 Valve accessories Valve head 1/16 stainless steel AVC28AC AVC38AC AVC26BC A1369 AVR28AC AVR38AC AVR26BC A1373 A position valve, 6-port, DLC stainless steel, Vespel, 1/16", 1200 bar, 0.3 mm bore size 2-position valve, 8-port, DLC stainless steel, Vespel, /16", 1200 bar, 0.3 mm bore size 2-position valve, 6-port, stainless steel, Vespel, 1/16", 400 bar, 0.3 mm bore size, 2-position valve, 6-port, stainless steel, Vespel, 1/16", 300 bar, 0.6 mm bore size Multiposition valve, 6-port, DLC stainless steel, Vespel, 1/16", 1200 bar, 0.3 mm bore size Multiposition valve, 6-port, DLC stainless steel, Vespel, 1/16", 1200 bar, 0.3 mm bore size Multiposition valve, 6-port, stainless steel, Vespel, 1/16", 400 bar, 0.3 mm bore size Multiposition valve, 6-port, stainless steel, Vespel, 1/16", 300 bar, 0.6 mm bore size Multiposition valve, 16-port, stainless steel, POM-H-TF, 1/16", 50 bar, 0.6 mm bore size Valve head 1/8 stainless steel A1371 A1375 A position valve, 6-port, stainless steel, Vespel, 1/8", 300 bar, 1.2 mm bore size Multiposition valve, 6-port, stainless steel, Vespel, 1/8", 300 bar, 1.2 mm bore size Multiposition valve, 12-port, stainless steel, POM-H-TF, 1/8", 25 bar, 1.2 mm bore size Valve head 1/16 PEEK A1370V1 A1374V1 2-position valve, 6-port, PEEK, PPS, 1/16", 150 bar, 0.6 mm bore size Mutliposition valve, 6-port, PEEK, PPS, 1/16", 150 bar, 0.6 mm bore size Valve head 1/8 PEEK A1372 A1376V1 2-position valve, 6-port, PEEK, PPS, 1/8", 150 bar, 1.2 mm bore size Multiposition valve, 6-port, PEEK, PPS, 1/8", 150 bar, 1.2 mm bore size Rotor seals A channel 1/16", POM-H-TF (50 bar) A channel 1/8", POM-H-TF (25 bar) A channel, 1/16" and 1/8" PPS (200 bar) A channel, 1/16" and 1/8" Vespel (300 bar) A channel, 1/16" Vespel (1200 bar) A channel, 1/16" Vespel (400 bar) A channel 1/16", PPS (200 bar) A channel 1/8", PPS (200 bar) A channel, 1/16" Vespel (1200 bar) A channel, 1/16" Vespel (300 bar) A channel, 1/16" Vespel (400 bar) A channel, 1/8" Vespel (300 bar) A channel, 1/16" Vespel (1200 bar) A Port 1 channel, 1/16" Vespel (1200 bar) 45

52 Valve accessories Sample loop stainless steel, 1/16, incl. fittings A05641 A05642 A05643 A05644 A05645 A05646 A05647 A05648 A0565 A0566 A0567 A0568 Sample Loop VICI,1 µl, stainless steel, 0.1 mm ID Sample loop,1 µl, stainless steel, 0.1 mm ID Sample loop, 2 µl, stainless steel, 0.1 mm ID Sample loop, 5µl, stainless steel, 0.25 mm ID Sample loop, 10 µl, stainless steel, 0.25 mm ID Sample loop, 20 µl, stainless steel, 0.25 mm ID Sample loop, 50 µl, stainless steel, 0.45 mm ID Sample loop, 100 µl, stainless steel, 0.45 mm ID Sample loop, 200 µl, stainless steel, 1 mm ID Sample loop, 500 µl, stainless steel, 1 mm ID Sample loop, 1000 µl, stainless steel, 1 mm ID Sample loop, 2000 µl, stainless steel, 1 mm ID A KNAUER VariLoop S, variable injection volume and multiple injections, movable piston protects against dilution, 10 ml, stainless steel A KNAUER VariLoop L, variable injection volume and multiple injections, movable piston protects against dilution, 40 ml, stainless steel A0564 A0568 Sample loop stainless steel, 1/8, incl. fittings A1043 A1044 A0586 A0843 Sample loop, 1 ml, stainless steel, 2.2 mm ID Sample loop, 2 ml, stainless steel, 1.6 mm ID Sample loop, 5ml, stainless steel, 2.2 mm ID Sample loop, 10ml, stainless steel, 2.2 mm ID A KNAUER VariLoop S, variable injection volume and multiple injections, movable piston protects against dilution, 10 ml, stainless steel A KNAUER VariLoop L, variable injection volume and multiple injections, movable piston protects against dilution, 40 ml, stainless steel A Sample loop PEEK, 1/16, incl. fittings A1058 A1060 A0508 A1061 A1057 A0423 A0785 Sample loop, 10 µl, PEEK, 345 bar, 0.25 mm ID Sample loop, 50 µl, PEEK, 240 bar, 0.7 mm ID Sample loop, 100 µl, PEEK, 240 bar, 0.7 mm ID Sample loop, 200 µl, PEEK, 240 bar, 0.7 mm ID Sample loop, 500 µl, PEEK, 240 bar, 0.7 mm ID Sample loop 1 ml, PEEK, 240 bar, 0.7 mm ID Sample loop 2ml, PEEK, 240 bar, 0.7 mm ID A0423 Sample loop PEEK, 1/8, incl. fittings A78980 A78985 A1929 A1928 Bioline Sample Loop 5ml, PEEK, 50 bar, 1/8 and 1/16, 1.6 mm ID Bioline Sample Loop 10ml, PEEK, 50 bar, 1/8 and 1/16, 1.6 mm ID Superloop, 50 ml, variable injection volumes and multiple injections, movable piston protects against dilution, 1/16, Glass, 40 bar Superloop, 150 ml, variable injection volumes and multiple injections, movable piston protects against dilution, 1/16, Glass, 20 bar A

53 Valve accessories Injection syringes for 1/16 injection port A0723 Injection syringe 10 µl A0724 Injection syringe 25 µl A0725 Injection syringe 50 µl A0726 Injection syringe 100 µl A0727 Injection syringe 250 µl A0728 Injection syringe 500 µl A0729 Injection syringe 1000 µl A0730 Injection syringe 2500 µl A0723 Luer-Lock glass syringes for 1/8 injection port A0573 A0653 Luer-Lock glass syringe, 10 ml Luer-Lock glass syringe, 20 ml A0653 A0328 Loop filling ports A0555 Loop filling port A0328 Injection Port, stainless steel, 1/16 A03281 Injection Port, PEEK, 1/16 A0505 Injection Port, stainless steel, 1/8 A05051 Injection Port, PEEK, 1/8 A0555 AZURA mounting brackets A Mounting bracket AZURA L for axial comp. column ID20 A Mounting bracket AZURA L for prep sample loop A Mounting bracket AZURA L for Knauer flow cells A9853 Mounting bracket AZURA L for Knauer manual injection valves A Mounting bracket AZURA L for Vici manual injection valves A Mounting bracket AZURA L for Vici valve drives A Mounting bracket AZURA L for Hypershear mixing chamber A Mounting bracket AZURA L Bio for manual KNAUER injection valve, ph-flowcell and 2 prepacked columns A Mounting bracket AZURA S for manual KNAUER injection valve A A

54 Services / Qualification Installation Qualification (IQ) - scope of delivery with all AZURA instruments VIQ_Inst IQ document for all instruments Installation Qualification (IQ) - software Built in software Operation Qualification (OQ) - pumps VOQ_Pumps OQ Pumps AZURA, Smartline, BlueShadow, Platinblue VIQ_Inst Operation Qualification (OQ) - autosampler VOQ_AS OQ Autosampler Operation Qualification (OQ) - detectors VOQ_DAD OQ AZURA DAD 6.1L, DAD 2.1L, MWD 2.1L VOQ_RF20 OQ RF20A/RF20Axs VOQ_RID OQ AZURA RID 2.1L, Smartline S2300 VOQUVD21SA OQ AZURA UVD 2.1S VOQUVD21LA OQ AZURA UVD 2.1L VOQCM21SA OQ AZURA CM 2.1S VOQ_Pumps Operation Qualification (OQ) - flow cells VOQ_Flow OQ Flow Cells Operation Qualification (OQ) - osmometer VOQ_K7400 OQ K-7400 VOQ_K7400S OQ K-7400S Operation Qualification (OQ) - valves VOQ_Valves OQ Valves VOQ_FRC Operation Qualification (OQ) - fraction collectors VOQ_FRC OQ for fraction collectors Operation Qualification (OQ) - column thermostat VOQCT21A OQ AZURA CT 2.1 Performance Verification (PV) VPV_001 VPV_002 AZURA system with UV detection AZURA system with RID detection VOQ_Valves 48

55 Accessories Fittings K-Connect system Kits consisting of bushings, clamping and sealing rings A9646 K-Connect Fingertight Fitting, PEEK, long, Set of 2, incl. ferrule, UNF 10/32 Thread for 1/16 inch K-Connect and PEEK Capillaries. A9645 K-Connect Fingertight Fitting, Stainless Steel, long, Set of 2, incl. ferrules, UNF 10/32 Thread for 1/16 inch K-Connect Capillaries. A9647 K-Connect Standard Fitting, Stainless Steel, Set of 2, incl. ferrule, UNF 10/32 Thread for 1/16 inch K-Connect Capillaries A K-Connect Standard Fitting, Stainless Steel, Set of 10, incl. ferrule, UNF 10/32 Thread for 1/16 inch K-Connect Capillaries. A9646 A9645 A9647/ A DYNASEAL system! Kits consisting of bushings, clamping and sealing rings A0108 DYNASEAL connection system, 1/16", 4 short bushings, 4 clamping rings and 8 sealing rings A0181 DYNASEAL connection system, 1/16", 3 long bushings, 3 clamping rings and 4 sealing rings A1020 A1069 DYNASEAL connection system, 1/16", 10 short bushings, 10 biconical sealing rings DYNASEAL connection system, 1/16", 5 long bushings, 5 biconical sealing rings A0736 DYNASEAL connection system, 1/8", M8x1, 4 long bushings, 4 clamping rings and 8 sealing rings A0644 DYNASEAL connection system, 1/8", M8x1, 4 short bushings, 4 clamping rings and 8 sealing rings A0108 A0181 A1020! Bushings for capillaries - DYNASEAL A1021 Bushings for 1/16" capillaries, Dynaseal system, stainless steel, fingertight, short, 10 pcs. A1064 Bushings for 1/16" capillaries, Dynaseal system, stainless steel, fingertight, long, 5 pcs. A1067 Bushings for 1/8" capillaries, Dynaseal system, stainless steel, fingertight, short, 4 pcs. A0735 Bushings for 1/8" capillaries, Dynaseal system, stainless steel, fingertight, long, 4 pcs. A1069 A1021 A1064 A0735 Split-grooved clamping rings A0484 A1239 A Split-grooved clamping rings for capillaries with 1/16" OD 4 Split-grooved clamping rings for capillaries with 1/8" OD 100 Split-grooved clamping rings for capillaries with 1/16" OD A0482 A0484 Sealing rings A0139 A0140 A1062 A0232 A Sealing rings for capillaries with 1/16" OD, polymer 100 Sealing rings for capillaries with 1/16" OD, polymer 10 Sealing rings for capillaries with 1/16" OD, PEEK 10 Sealing rings for capillaries with 1/8" OD, polymer 10 Sealing rings for capillaries with 1/8" OD, PEEK A0139 A0140 Biconical sealing rings A1070 A1022 A Biconical sealing rings with 1/16", PEEK 10 Biconical sealing rings with 1/16", polymer 10 biconical sealing rings 1/8" PETP A

56 Accessories Fittings Bushings for capillaries 1/16, stainless steel A Bushings for capillaries with 1/16" OD, stainless steel, wrench caliber 1/4", UNF 10-32, short A Bushings for capillaries with 1/16" OD, stainless steel, wrench caliber 1/4", UNF 10-32, short A Bushings for capillaries with 1/16" OD, stainless steel, wrench caliber 1/4", UNF 10-32, long A Bushings for capillaries with 1/16" OD, stainless steel, wrench caliber 1/4", UNF 10-32, long A0112/A0113 A0115/A0116 Bushings for capillaries 1/8, stainless steel A Bushings for capillaries with 1/8" OD, M8x1, wrench caliber 10, stainless steel A0830 Ferrules for capillaries A0110 A0111 A0874 A01101 A Ferrules for capillaries with 1/16" OD, stainless steel 100 Ferrules for capillaries with 1/16" OD, stainless steel 10 Ferrules for capillaries with 1/8" OD, stainless steel 10 Ferrules, Hastelloy, for capillaries with 1/16" OD 10 Ferrules, Titanium, for capillaries with 1/16" OD A0110/A0111 Bushings for capillaries PEEK & polymer A0141 A0142 A0144 Bushings for 1/16" capillaries, polymer, knurled, UNF 10-32, short, 10 pcs. Bushings for 1/16" capillaries, polymer, knurled, UNF 10-32, short, 30 pcs. Bushings for 1/16" capillaries, polymer, knurled, UNF 10-32, long, 10 pcs. A0145 Bushings for 1/16" capillaries, polymer, with integrated sealing cone, knurled, UNF 10-32, short, 10 pcs. A0584 A0733 A25011 A25021 Bushings for 1/16" capillaries, PEEK, UNF 10-32, 10 pcs. Bushings for 1/8" capillaries, polymer, with integrated sealing cone, M8x1, knurled, short,10 pcs. Bushing for 1/16" capillaries, PEEK, short Hex, with integrated sealing cone, 5 pcs. Bushing for 1/16" capillaries, PEEK, long Hex, with integrated sealing cone, 5 pcs. A0141 A25011 A0733 A25012 Flat bottom fittings and adapters A5829 A58291 A58292 A58293 A58294 A1982 Bushings flat bottom for 1/8" capillaries, PEEK, Super flangeless, 1/4-28, 10 pcs. Bushings flat bottom for 1/16" capillaries, PEEK, Super flangeless, 1/4-28, 10 pcs. Ferrules for 1/16" capillaries and flat bottom bushings, PEEK, with lock ring (stainless steel), for Super flangeless bushings, 10 pcs. Ferrules for 1/8" capillaries and flat bottom bushings, PEEK, with lock ring (stainless steel), for Super flangeless bushings, 10 pcs. Ferrules for 1/8" capillaries and flat bottom bushings, ETFE, with lock ring (stainless steel), for Super flangeless bushings, 10 pcs. Adapter PEEK 1/16" external 10/32 thread on 1/8" flat bottom internal thread A58292 A58294 A5829 A58291 A1982 A05841 Adapter PEEK 1/8" external thread on 1/16" flat bottom internal thread A

57 Accessories - Caps/connectors Caps (blind fittings) A0146 A0147 A0582 A0734 Blind fittings, 1/16", polymer, knurled, UNF 10-32, short, 10 pcs. Blind fittings, 1/16", polymer, knurled, UNF 10-32, short, 30 pcs. Blind fittings, 1/16", PEEK, knurled, UNF 10-32, short, 10 pcs. Blind fittings, 1/8", polymer, knurled, M8x1, short, 10 pcs. A0146 A0734 Low dead volume couplings, polymer/peek A0148 A0149 A0233 A1407 A14071 Low dead volume coupling to connect 2 capillaries with 1/16'' OD (material: PEEK/ PETP, thread: UNF10-32), including 2 bushings and sealing rings, 1 pc. Low dead volume coupling to connect 2 capillaries with 1/16'' OD (material: PEEK/ PETP, thread: UNF10-32), including 2 bushings and sealing rings, 5 pcs. Low dead volume coupling to connect 2 capillaries with 1/16'' OD (material: PEEK, thread: UNF), including 2 one-piece PEEK fittings Low dead volume coupling to connect 2 capillaries with 1/16'' and 1/8"OD (material: PEEK, thread: UNF, M8x1), including 2 one piece fittings (1x 1/16", 1x 1/8") Low dead volume coupling to connect 2 capillaries with 1/8"OD (material: PEEK, thread: M8x1), including 2 one piece fittings 1/8" A0148 A0233 Low dead volume couplings, stainless steel/titanium A0117V1 A0117 A0118 A0119 A2512 A2513 A0485 A0480 Low dead volume coupling to connect 2 capillaries with 1/16'' OD (material: titanium, thread: UNF), including 2 bushings and ferrules Low dead volume coupling to connect 2 capillaries with 1/16'' OD (material: stainless steel, thread: UNF), including 2 bushings and ferrules Low dead volume coupling to connect 2 capillaries with 1/16'' OD (material: stainless steel, thread: UNF), including bushings and ferrules, 5 sets Low dead volume coupling to connect 2 capillaries with 1/16'' OD (material: stainless steel, thread: UNF), including bushings and ferrules, 25 sets Low dead volume coupling to connect 2 capillaries with 1/8" OD (material: stainless steel, thread: M8x1), including 2 bushings and ferrules Low dead volume coupling to connect a capillary with 1/16" OD to a capillary with 1/8'' OD (material: stainless steel, thread: M8x1, UNF) Low dead volume coupling Dynaseal to connect a capillary with 1/16" OD to a capillary with 1/8'' OD (material: stainless steel, thread: M8x1, UNF), including Dynaseal bushings and ferrules (1x 1/16", 1x 1/8") Low dead volume coupling Dynaseal to connect 2 capillaries with 1/8" OD (material: stainless steel, thread: M8x1), including 2 Dynaseal bushings and ferrules A0117 A2513 Unions and Reducing unions, stainless steel A58263 A58263 Union to connect 2 capillaries with 1/4" OD A58264 Reducer to connect a capillary with 3/8" OD to a capillary with 1/4'' OD A58264 A58265 Reducer to connect a capillary with 8 mm OD to a capillary with 1/4'' OD A58265 A58266 Reducer to connect a capillary with 1/8'' OD to a capillary with 1/4'' OD A58266 A58270 Reducer to connect a capillary with 1/16'' OD to a 1/8'' OD pipe A58271 Reducer to connect a capillary with 1/8'' OD to a 1/4'' pipe union A58271 A

58 Accessories - Caps/connectors T-connectors, stainless steel /titanium A2511 T-connector to connect 3 capillaries with 1/8'' OD (material: stainless steel, thread: M8x1), including 3 bushings and ferrules A0120 T-connector to connect 3 capillaries with 1/16'' OD (material: stainless steel, thread: UNF), including 3 bushings and ferrules A58260 A58261 A58262 T-connector to connect 3 capillaries with 1/8'' OD (material: stainless steel) T-connector to connect 3 capillaries with 1/4'' OD (material: stainless steel) T-connector to connect 3 capillaries with 1/4'' OD (material: titanium) A58260 A58261 A58262 A2511 T-connectors, polymer A0150 T-connector to connect 3 capillaries with 1/16'' OD (material: PETP/POM, thread: UNF), inclusive 2 bushings and sealing rings A T-connector to connect 3 capillaries with 1/8'' OD (material: PEEK, thread: M8x1),including 2 one piece PEEK fittings 1/8" A0150 X-connectors, stainless steel A0121 X-connector to connect 4 capillaries with 1/16'' OD (material: stainless steel, thread: UNF), including 4 bushings and ferrules A1096 X-connector to connect 4 capillaries with 1/8'' OD (material: stainless steel, thread: M8x1), including 4 bushings and ferrules A58272 X-connector to connect 4 tubings with 1/4'' OD (material: stainless steel, Swagelok) for 1000 ml/ min Systems A1096 X-connectors, polymer A0151 X-connector to connect 4 capillaries with 1/16'' OD (material: polymer, thread: UNF), including 4 bushings and sealing rings Pressure release valves A5800 Pressure Release Valve for AZURA pump P 2.1L and Pump 1800 (up to 50 bar), 1/8", stainless steel A5800V1 Pressure Release Valve for AZURA pump P 2.1L and Pump 1800 (up to 50 bar), 1/8", titanium A5801 Pressure Release Valve for AZURA pump P 2.1L and Pump 1800 (up to 25 bar), 1/8", stainless steel A5802 A5805 M1070 M1080 Pressure Release Valve for AZURA pump P 2.1L and Pump 1800 (without spring), 1/4", stainless steel Pressure release valve stainless steel, up to 3 bar, 1/16" connectors Spring for pressure release valve, Series "R3A" bar Spring for relief valve Series "R3A" bar M1258 Pressure relief valve tee for 1/16" OD Tubing, incl. fittings for 1/16" M1080 A0151 A580* M1070 Backpressure regulators A70087 Back-Pressure Regulator for 1/16 OD tubing, threads, PEEK, Range 1-20 bar ( psi) A70088 Back-Pressure Regulator for 1/16 OD tubing, threads, PEEK, Range bar ( psi) A70084 Back-Pressure Regulator for 1/16 OD tubing, threads, stainless steel, Range bar ( psi) A70082 Spare membranes for Back-Pressure Regulators A70084, A70087, A70088 A5804 Back-Pressure Regulator for higher flowrates up to 220 ml/min, for 1/8" and 1/16" OD tubing, 134 µl volume, PEEK, provides a constant back-pressure of 1.4 bar (20 psi), contains fittings for 1/8" and 1/16" A70087 A

59 Accessories - Capillaries Capillaries, stainless steel, 1/16" Amount ID Length A Capillary 0.10 mm 300 cm A Capillary 0.25 mm 300 cm A Capillary 0.50 mm 300 cm A Capillary 0.70 mm 300 cm A Capillary 1.00 mm 300 cm A Capillaries 0.10 mm 10 cm A Capillaries 0.10 mm 20 cm A Capillaries 0.10 mm 30 cm A Capillaries 0.25 mm 10 cm A Capillaries 0.25 mm 20 cm A Capillaries 0.25 mm 30 cm Capillaries, titanium, 1/16" Amount ID Length A Capillary 0.70 mm 50 cm Capillaries, stainless steel, 1/8" Amount ID Length A Capillary 1.60 mm 150 cm A Capillary 2.20 mm 150 cm AZURA Analytical K-Connect start-up kits, stainless steel, 1/32" capillaries with fitting sleeves for 1/16"connections. Sets include: 2 capillaries 150 mm, 2 capillaries 400 mm, 2 capillaries 700 mm, 1 Connector UNF 10/32 - UNF 10/32 stainless steel, 1 Connector UNF 10/32 - UNF 10/32 PEEK, 10 Wrench-tight fittings stainless steel, 2 Finger-tight fittings stainless steel, 2 Finger-tight fittings PEEK, 5 adapters for AZURA L-devices capillary guidance Color code AZF40 red 0.10 mm AZF50 yellow 0.18 mm AZF60 black 0.45 mm ID Capillaries, AZURA Analytical K-Connect, stainless steel, 1/32" with fitting sleeve for 1/16"connections Color code ID Length AZF41 red 0.10 mm 150 mm AZF42 red 0.10 mm 300 mm AZF43 red 0.10 mm 400 mm AZF44 red 0.10 mm 700 mm AZF45 red 0.10 mm 900 mm AZF51 yellow 0.18 mm 150 mm AZF52 yellow 0.18 mm 300 mm AZF53 yellow 0.18 mm 400 mm AZF54 yellow 0.18 mm 700 mm AZF55 yellow 0.18 mm 900 mm AZF61 black 0.45 mm 150 mm AZF62 black 0.45 mm 300 mm AZF63 black 0.45 mm 400 mm AZF64 black 0.45 mm 700 mm AZF65 black 0.45 mm 900 mm 1/32 1/16 AZURA Capillary start-up kit, stainless steel A9849 AZURA start-up kit 1/16" stainless steel, capillary kit A AZURA start-up kit 1/16", stainless steel, semi-prep, capillary kit A9850 AZURA start-up kit 1/8" stainless steel, capillary kit AZF70 AZURA start-up kit 1/16" stainless steel, capillary kit, 0.25 mm ID precut capillaries 53

60 Accessories - Tubings Tubing start-up kits for FPLC A70500 A70500A A70600 A70300 A70300A A70300B A70400 AZURA FPLC Start-up kit, PEEK, 1/16" for 10 ml/min FPLC systems AZURA FPLC Start-up kit, transparent FEP, 1/16" for FPLC systems up to 10 ml/min and 20 bar AZURA FPLC Start-up kit, PEEK/Tefzel, 1/16" for 50 ml/min FPLC systems AZURA FPLC Start-up kit, FEP/PEEK, 1/8" for 100 ml/min ml/min FPLC systems AZURA FPLC Start-up kit, PEEK, 1/8" for 100 ml/min ml/min FPLC systems, up to 100 bar. AZURA FPLC Start-up kit, PEEK/Tefzel, 1/16" for FPLC systems up to 100 ml/min AZURA FPLC Start-up kit, 1/4" for 1000 ml/min FPLC systems PEEK tubing, 1/16", variable length, price per meter Color code ID max. pressure A2522 red striped 0.13 mm 420 bar A2523 yellow striped 0.18 mm 400 bar A2524 blue striped 0.25 mm 385 bar A2525 orange striped 0.50 mm 350 bar A2526 green striped 0.70 mm 240 bar A2527 grey striped 1.0 mm 165 bar A2528 black striped 1.4 mm 50 bar PEEK tubing, 1/8" OD, variable length, price per meter Color code ID max. pressure A2540 natural 1.59 mm 220 bar A2541 natural 0.75 mm 345 bar A2542 natural 2.00 mm 165 bar Tefzel tubing, 1/16" OD ID Length max. pressure A mm 300 cm 185 bar A mm 300 cm 115 bar A mm 1000 cm 115 bar A mm 300 cm 85 bar ETFE tubing, 1/8" OD ID Length max. pressure A mm 300 cm 70 bar PTFE tubing, various OD OD ID Length max. pressure A0152 1/16" (1.6 mm) 0.45 mm 300 cm 150 bar A /16" (1.6 mm) 0.9 mm 300 cm n/a A mm 1.5 mm 300 cm < 10 bar A0732 1/8" (3.2 mm) 1.5 mm 300 cm 35 bar A mm 2.0 mm 300 cm n/a A mm 3.0 mm 300 cm 20 bar A mm 7 mm 300 cm n/a 54

61 Accessories - Inline filters / osmometry Inline filters, stainless steel, for HPLC A3381 Inline Filter (prep.) 5-10 µm, stainless steel, max. flow rate 1000 ml/min (for 1/8" tubing) A33811 Replacement frit for A µm, stainless steel, max. flow rate 1000 ml/min A00161 Inline Filter, PEEK body, Stainless steel frit, 1/16", to protect your column, with 2 µm pore size, 3 pcs., easily connected directly to any column B2 UHPLC/HPLC precolumn filter, universal, 0.5 µm titanium frit, set of 5, stainless steel body, up to 1034 bar A00161 B2 Inline filters, biocompatible, for FPLC A3378 Inline Filter, PEEK/Titanium, 1/16", biocompatible, to protect your column, with 2 µm pore size titanium frit A3379 Inline Filter, PEEK/Titanium, 1/16", biocompatible, to protect your column, with 10 µm pore size titanium frit A Replacement Frits 2 µm for Inline Filter, PEEK/Titanium, biocompatible, for A3378 A Replacement Frits 10 µm for Inline Filter, PEEK/Titanium, biocompatible, for A3379 A00162 Inline Filter, PEEK body, Titanium frit, 1/16", to protect your column, with 0.5 µm pore size, 3 pcs.,easily connected directly to any column A00163 Inline Filter, PEEK body, Titanium frit, 1/16", to protect your column, with 2 µm pore size, 3 pcs., easily connected directly to any column A3378 A A00161/2/3 Shut-off valves A5811 A5812 Shut-off valve, PEEK, 1/16", including connectors (1/4-28 flat bottom) Shut-off valve, PEEK, 1/8", including connectors (1/4-28 flat bottom) A5811 Adapters A1980 Luer Adapter to 10-32, ETFE, female Luer to male 10/32 threads for injection, simply screw the adapter in the port of your injection valve A1980 Osmometry accessories A01240 Pack of 12 ampules NaCl calibrating solution, 300 mosmol/kg A01241 Pack of 10 ampules NaCl calibrating solution, 400 mosmol/kg A01250 Pack of 12 ampules NaCl calibrating solution, 850 mosmol/kg A01248 Pack of 12 ampules NaCl calibrating solution, 2000 mosmol/kg A02330 Cleaning tissue, lint-free, for thermistor cleaning A Measuring head for plastic sample tubes A

62 Accessories - Eluent and column heating / purification High flow eluent and column heating devices A70053V1 A70054V4 A70054V3 A70058 A57024 A57026 A57027 A57028 A57029 A57030 A57031 A57032 Eluent heating element 1/16" Capillary, 1mm ID integrated PT100 Temp.Sensor Temp. protect switch Interface for temperature control and evaluation for A70053V1 2-channel temp. control for clean room Interface for temperature control and evaluation for A70053V1 1-channel temp. control for clean room Software for temperature control and logging Temperature controller for heating sleeve 2300 W (10 A) 230 VAC Heating sleeve for HPLC column 150 x 20 mm HM D=25..57*L=193 mm 100 C, 230V, 200W, Pt100 Heating sleeve for HPLC column 250 x 20 mm HM D=25..57*L=293 mm 100 C, 230V, 200W, Pt100 Heating sleeve for HPLC column 150 x 30 mm HM D=38..70*L=203 mm 100 C, 230V, 400W, Pt100 Heating sleeve for HPLC column 250 x 30 mm HM D=38..70*L=303 mm 100 C, 230V, 500W, Pt100 Heating sleeve for HPLC column 150 x 50 mm HM D= *L=211 mm 100 C, 230V, 500W, Pt100 Heating sleeve for HPLC column 250 x 50 mm HM D= *L=311 mm 100 C, 230V, 800W, Pt100 Heating sleeve for HPLC costum made up to 350 x 50 mm A70053V1 A570XX Purification AZG10 AZG10-1 AZG10-2 Pressure Control for delta P measurement up to 250 ml/min with interface box External pressure sensor up to 250 ml/min External pressure sensor up to 1000 ml/min A70092 Air sensor (1/16") for AZURA Bio LC with one airsensor and wiring for up to 4 airsensors A Additional Air sensor for AZURA Bio LC for 1/16" tubing A70093 Air sensor (1/8") AZURA Bio LC with one airsensor and wiring for up to 4 airsensors A Additional Air sensor for AZURA Bio LC for 1/8" tubing A70083 Air sensor (1/4 ) for AZURA Bio LC with one airsensor and wiring for up to 4 airsensors A Additional Air sensor for AZURA Bio LC for 1/4" tubing AZS80SA Distribution Box 24 V for 6 devices like Air sensor, Pressure Control, IFU 2.1 LAN A70085 AZURA Organizer Holder for AZURA Click for attaching falcons, columns (5 mm - 26 mm OD), A Clamp for AZURA Organizer 16 mm A Clamp for AZURA Organizer 16 mm A Clamp for AZURA Organizer 25 mm A70089 AZURA Click rail for AZURA L devices for attaching IFU 2.1 LAN, airsensor, pressure control AZG10 AZG10-2 A70092 A5813 Flow splitter for use of CM 2.1S over 100 ml/min A70089 A

63 Accessories - Vials / caps kits Vials and caps kits for analytical HPLC A0637 Vials: Screw neck vials N8 (ø 11 mm), 1.5 ml, clear glass, flat bottom, small opening Caps: Screw Caps N8 PP, black with center hole Septa: PTFE white, thickness 0.25 mm 1000 pcs. each A Vials: Screw neck vials N8 (ø 11 mm), 1.5 ml, clear glass, flat bottom, small opening Caps: Ready assembled screw closures, septa are assembled, caps are ready-to-use, Screw Caps N8 PP, black with center hole Septa red rubber/fep colorless 1000 pcs. each A Vials: Screw neck vials N8, (ø 11 mm), 1.5 ml, amber glass, with label field Caps: Screw Caps N8 PP, black, center hole, Septa: PTFE white, thickness 0.25 mm 1000 pcs. each A0638 A0664 Vials: Crimp neck vials N11 (ø 11 mm), 1.5 ml, clear glass, wide opening, flat bottom, Caps: Crimp caps aluminium, center hole, ready assembled with septa rubber/tef 1000 pcs. each Vial kit for analytical autosamplers: Vials: Crimp neck vials N11 (ø 11 mm), 1.5 ml, clear glass, wide opening, flat bottom, Caps: Crimp caps aluminium, center hole, ready assembled with septa rubber/tef 1000 pcs. each including pliers for opening and closing A0637 A A A0638 Vials and caps kits including microinserts for analytical HPLC A Vials: Screw neck vials N9 (ø 11 mm), 1.5 ml, amber glass, flat bottom Inlays: 0.3 ml glass micro inserts Caps: Screw caps blue with septum silicone white/ptfe red 100 pcs each A Vials: Screw neck vials N9 (ø 11 mm), 0.2 ml, amber glass, flat bottom, incl. integrated microinserts Caps: Screw caps blue Septa: Silicone white/ptfe red 100 pcs each A0664 Vials and caps kits for preparative HPLC A1585 A15851 Vials: Crimp neck vials N20 (ø 22 mm), 10 ml, clear glass Caps: Cap PE transparent, N20 for flat crimp neck, center hole, Septa: Natural rubber red-orange/tef, 1000 pcs. each Vials: Crimp neck vials N20 (ø 22 mm), 10 ml, clear glass Caps: Cap PE transparent, N20 for flat crimp neck, center hole, Septa: Natural rubber red-orange/tef, 100 pcs. each A A1585 A15851 Pliers for analytical and preparative vials A0864 A0865 A1660 A1661 Closing pliers for crimpcaps, for ø 11 mm vials Opening pliers for crimpcaps, for ø 11 mm vials Closing pliers for crimpcaps, for ø 20 mm vials Opening pliers for crimpcaps, for ø 20 mm vials A0864 Sample tubes for osmometry A02721 A0272 A0720 Plastic sample tubes for Semi-Micro Osmometer K-7400S, 100 pcs. Plastic sample tubes for Semi-Micro Osmometer K-7400S, 500 pcs. Plastic sample tubes for Semi-Micro Osmometer K-7400S, 1000 pcs. A02721 A0272 A

64 Accessories - Tools Wrenches and tightening tools X0219 X0220 X0221 X0222 Torque wrench basic tool, 1-25 Nm, without plug-in head Open-jaw plug-in head 1-17 mm (for ml pump head in-/outlet and LPGblock) Open-jaw plug-in head 1-10 mm (for Smartline I pump heads) Open-jaw plug-in head 1-13 mm (for ml Smartline II/AZURA pump heads in-/outlet) X0003 Double open-end wrench, 1/4" and 5/16" X0030 Double open-end wrench, 8/10 mm A0138 Double open-end wrenches, 2 pc., 1/4"and 5/16" A25030 Tightening tools for PEEK fittings, blue, 1/16 fittings 1/4 hex head nut (10-32 threads) A25031 Tightening tools for PEEK fittings, green, 1/32 fittings 3/16 hex head nut (6-40 threads) A25030 A25031 X0219 X0030 A0569 Capillary and tube cutter A0569 Tube cutter, suitable for all tubes A0851 Capillary cutter for PEEK capillaries and tubings with OD up to 4 mm A0809 Metal capillary cutting pliers for 1/16" capillaries A9865 Metal capillary cutter for 1/8" capillaries A0851 A0809 A9865 Capillary grater and bender A0137 A9864 Capillary grater for degrating of 1/16" stainless steel capillaries, can also be used to remove column filters Capillary grater for degrating of 1/8" stainless steel capillaries A9870 Tube bender for 1/8" and 3/16" tubings with an bend radius of 90 A0137 A9864 A9870 Tool sets A1033 Tool Kit AZURA for systems with PEEK or pre-cut capillary kits A Tool Kit AZURA for 1/16" systems (stainless steel) A Tool Kit AZURA for 1/8" systems (stainless steel) A

65 Accessories - Racks / column holder LC racks A70010 A70011 A70015 A9860 Benchtop rack: AZURA L low 480 x 420 x 190 mm Benchtop rack: AZURA L high 480 x 420 x 430 mm Benchtop rack: special manufacture with customized dimensions Product Riser AZURA : Set of 4 feet that lift the device to a height of 28 mm for easy handling of the waste tube of the drainage system A70010 A9860 A9847 A3983 A1319 LC column holder/multi column base A9847 A3983 A1319 A70190 A0070A Column holder: Magnetic clip, for all columns with 1/4" OD, for all KNAUER columns with 3, 4 and 4.6 mm ID, compatible with all AZURA devices Prism column holder for horizontal storage of HPLC columns on the lab bench, the most price attractive alternative to store your HPLC columns Glass column holder, Stand, plate and 2 clamps, can hold one glass column in the dimensions of mm ID Multi Column Base Bio 60 x 40 x 130cm (w x d x h) for up to 3 MPLC columns with conn. for cooling device Multi Column Base serves as a holder for up to 3 columns with inner diameter up to 50 mm, especially made for preparative column solutions A4363 A2820 A2820A A70190 A0070A Accessories for LC column holder A0714A A4363 A4368 A2820 A2820A 4-finger clamps, long shaft finger with silicone coating ø12mm clamp width one piece 4-finger clamps, short shaft finger with silicone coating ø12mm clamp width one piece Clamp for Multi Column Base, short shaft, to fix an HPLC column or other accessories to the Multi Column Base Clamp for Multi Column Base, long shaft, to fix an HPLC column or other accessories to the Multi Column Base Bosshead squared for Multi Column Base, used in combination with clamps with a long shaft Installation Accessories A1071 HPLC Standard accessory kit A9862 Installation Box Kit, Box for small parts, KNAUER file folder and support sticker A1071 A

66 Applications & Academy With profound application knowledge of analytical and preparative HPLC and FPLC, our team is at your service around the world. Our experts are pleased to receive your inquiries and requests, and will offer attractive customized solutions. Visit our website for more information: Academy HPLC trainings and specialized courses for beginners and experienced users. Rent an expert Rent an expert for assistance in your lab. Method transfer & optimization Transfer your methods to one of our HPLC systems or make your analyses faster, more efficient and cost effective. Application development Proof of concept and support in selecting a HPLC / FPLC method for your separation task. Science projects KNAUER is involved in different research projects. Contact us for requests. Investigation KNAUER participates in different research projects. Ordering details: AL0200 AL0100 AL0700 AL0510 AL0520 AL0500 HPLC Application Development, at KNAUER in Berlin HPLC Method Transfer & Optimization, at KNAUER in Berlin Rent an Expert - Application Support, at customers site HPLC Troubleshooting Workshop, at KNAUER in Berlin HPLC Basics Workshop, at KNAUER in Berlin Academy Individual Workshop, at KNAUER in Berlin 60

67 Supported devices Device support in KNAUER CDS / KNAUER device support in 3rd party software OpenLAB CDS EZChrom Edition / ClarityChrom / Chromeleon / ChromGate / PurityChrom The following data has been retrieved on 07/17/2017. For up-to-date information, please visit the KNAUER website: The tables list the software package and driver version (in case multiple versions exist); the version number listed is the first version that supports the respective device. Limitations are marked with superscript, e.g. 1). n/a means, that a device is not supported, - that no information is available. If a device is supported by different communication ports, the desired port must be selected in the device directly. For some time, ChromGate was also available as PLATINblue Edition for the PLATINblue devices, while the standard version is named as regular. From driver version 2825 all drivers are available in the regular version; the PLATINblue Edition was discontinued. OpenLAB CDS EZChrom Edition version number lists Shared Service version and EZChrom version. KNAUER OEM devices are not supported by the KNAUER drivers. Pumps AZURA Pump P 6.1L AZURA Pump P 2.1L OpenLAB CDS EZChrom Edition CDS version and Driver Version OLEE A.01.05/ A Driver 1022 OLEE A.01.05/ A Driver 1017 ClarityChrom CDS version and Driver Version CC Driver ) CC Driver ) Chromeleon CDS version and Driver version Cm 7.2 Regular Class 3 driver 1) Cm 7.2 Regular Class 3 driver 1) Cm 6.8 Generic driver 2) ChromGate CDS version and Driver Version n/a CG Driver ) PurityChrom Version: ) Version: ) AZURA Pump P 2.1S/P 4.1S 6) PLATINblue Pump P-1 Smartline Pump ) Driver 1017 Driver 1017 Driver 1017 CC Driver 3078 CC Driver 4102 CC Driver 1100 Cm 6.8 Generic driver 2) Cm 6.8 SR10 Regular Class 3 driver Cm 6.8 SR10 Regular Class 3 driver Smartline Pump 1000 n/a CC Cm 6.8 Generic driver 2) CG Driver 1787 Version: CG PLATINblue n/a Edition 1751; Regular 2825 CG Driver 1751 Version: CG 3.1 n/a Smartline Pump 100 5), 6) Preparative Pump 1800 (also WellChrom K-1800) WellChrom Pump K-1001 WellChrom Pump K-1000 WellChrom Pump K-501 9) WellChrom Pump K-120 WellChrom Pump K-1001 BlueShadow Pumps 10P/20P 6) BlueShadow Pump 40P 10) BlueShadow Pump 80P 10) Driver 1017 CC 2.6 Cm 6.8 Generic driver 2) CG Driver 1640 Version: n/a CC 2.6 n/a CG 3.1 n/a n/a CC 2.6 n/a CG ) n/a n/a CC 2.6 n/a CG 2.55 (not supported n/a in version 3.1) 8) n/a CC 2.6 n/a CG 2.8 n/a n/a CC 2.6 n/a CG 2.8 n/a n/a CC 2.6 n/a CG ) n/a Driver 1017 Driver 1022 Driver 1022 CC Driver 3078 Cm 6.8 Generic driver 2) CG Driver 1787 Version: CC Driver 4095 n/a n/a Version: CC Driver 4095 Cm 6.8 Generic driver 2) n/a Version:

68 Supported devices Pumps Kontron Pumps 320/322/325 Kontron Pumps 420/422/425 Kontron Pumps 520/522/525 OpenLAB CDS EZChrom Edition CDS version and Driver Version ClarityChrom CDS version and Driver Version Chromeleon CDS version and Driver version ChromGate CDS version and Driver Version n/a CC ) - CG Driver ) n/a CC ) - CG Driver ) n/a CC ) - CG Driver ) PurityChrom n/a Version: n/a 1) constant Pressure Mode / Isobar Mode is not supported 2) generic drivers do not support all of the devices features (e.g. no Isobar Mode); only control via RS-232; should also work with Chromeleon 7.x 3) support of RS-232 port from driver version ) for control via RS-232 only baud rate of is supported 5) can be controlled as pump K-120 only via RS-232 without pressure sensor in former software versions 6) to set a static IP address a 3rd party tool is required 7) from ChromGate version 3.1 only with firmware version 5.xx or higher; pumps with firmware up to version 4.XX must be configured as K ) from ChromGate version 3.1 no pressure information; also K-1001 with firmware up to version 4.XX, no pressure trace Detectors (for all PDA or scanning UV detectors the 3D license option is required to acquire and review the scan (3D) data) AZURA Detector UVD 2.1L 1) AZURA Detector MWD 2.1L AZURA Detector UVD 2.1S 2) AZURA Detector DAD 6.1L AZURA Detector DAD 2.1L AZURA Detector RID 2.1L AZURA Conductivity Monitor CM 2.1S 2) PLATINblue Detector MW-1 3) PLATINblue DAD PDA-1 Smartline UV Detector ) Smartline UV Detector ), 7) OpenLAB CDS EZChrom Edition CDS version and Driver Version Driver 1017 driver 2068 Driver 1017 driver 2047 driver 2068 Driver 2092 driver 1022 Driver 1017 Driver 1017 ClarityChrom CDS version and Driver Version Chromeleon CDS version and Driver version CC Driver 3078 Cm 7.2 Regular Class 3 driver; Cm 6.8 Generic driver 5) CC Driver 5011 Cm 7.2 Regular Class 3 driver ChromGate CDS version and Driver Version CG Driver 2825 PurityChrom n/a n/a Version: ) CC Driver 3055 n/a CG Driver 1787 Version: CC Driver 4110 Cm 7.2 Regular Class 3 driver CC Driver 5011 Cm 7.2 Regular Class 3 driver CC Driver 5037 (compatible with CC/ CCP 5.x versions) Cm 7.2 Regular Class 3 driver CC Driver 4095 n/a Generic Driver June ) CC6.1.0; Driver 5015 CC Driver ) Cm 6.8 SR10 Regular Class 3 driver Cm 6.8 SR10 Regular Class 3 driver n/a CC Cm 6.8 Generic driver 5) Driver 1017 n/a Version: ) n/a Version: ) n/a Version: ) CG PLATINblue Edition 1751; Regular 2825 Version: ) n/a CG 3.3.2; n/a PLATINblue Edition 1751; Regular 2825 CG 3.1 Version: CC Driver 3033 n/a CG Driver 1751 n/a 62

69 Supported devices Detectors (for all PDA or scanning UV detectors the 3D license option is required to acquire and review the scan (3D) data) Smartline UV Detector ), 7) Smartline UV Detector 2600 Smartline UV Detector 200 Smartline PDA Detector 2800 Smartline PDA Detector 2850 Smartline RI Detector 2300 Smartline RI Detector 2400 Smartline Conductivity Monitor S2900 WellChrom UV Detector K ) WellChrom UV Detector K-2001 WellChrom UV Detector K ) WellChrom UV Detector K ) WellChrom UV Detector K-2600 WellChrom PDA Detector K ) WellChrom PDA Detector K ) WellChrom RI Detector 2300 WellChrom RI Detector 2301 WellChrom RI Detector 2400 WellChrom RI Detector 2401 Shimadzu fluorescence detector RF-10Axl 10) Shimadzu fluorescence detector RF-20A/Axs with CBM-20 Virtual Detector BlueShadow Detector 10D 3) BlueShadow Detector 40D 3), 7 OpenLAB CDS EZChrom Edition CDS version and Driver Version Driver 1017 ClarityChrom CDS version and Driver Version CC Driver 1079 Chromeleon CDS version and Driver version Cm 6.8 SR10 Regular Class 3 driver ChromGate CDS version and Driver Version CG Driver 1640 PurityChrom CC Cm 6.8 Generic driver CG 3.1 Version: Driver ) n/a CC 2.6 n/a CG Driver 1466 n/a n/a CC n/a CG Driver 446 n/a Driver 1017 Driver 1017 Driver 1017 Driver 1017 CC Driver 1100 n/a CG Driver 1751 n/a CC n/a CG 3.1 n/a CC n/a CG 3.1 n/a CC Driver 3055 n/a Generic Driver September 2010 n/a CC 2.6 n/a CG 2.8 n/a n/a CC 2.6 n/a CG 2.8 n/a n/a CC 2.6 n/a CG 2.8 n/a n/a CC 2.6 n/a CG 2.8 Driver 2026 CC 2.6 Cm 6.8 Generic driver 5) CG 2.55 n/a PCI / LAN n/a CG 2.55, LAN from n/a 3.1 n/a PCI / LAN n/a CG 2.8, LAN from 3.1 n/a n/a CC 2.6 n/a CG 2.8 n/a n/a CC 2.6 n/a CG 2.8 n/a n/a CC 2.6 n/a CG 2.8 n/a n/a CC 2.6 n/a CG 2.8 n/a n/a CC 2.6 n/a CG 3.1 Version: Shimadzu Control with official Shimadzu driver via CBM 20; requires special license, driver not developed by KNAUER Driver 1017 Driver 1017 driver 1022 CC 7.2.0, only LC-10 functionality, driver developed by Data Apex Cm 7.2 with official Shimadzu driver via CBM 20; requires special license, driver not developed by KNAUER CC Driver 1079 n/a CG n/a n/a CC Driver 3055 n/a CG Driver 1787 CC Driver 4095 n/a n/a n/a n/a n/a n/a n/a Version:

70 Supported devices Detectors (for all PDA or scanning UV detectors the 3D license option is required to acquire and review the scan (3D) data) Antec Leyden LC-EC Detector Decade Alltech Conductivity Detector 650 Kontron UV Detector 332/335 8), 12) Kontron UV Detector 430/430A/432/435 8), 12) Kontron UV Detector 530/532/535 8), 12) Kontron DAD 540(/ 545) 12) Sedere Sedex ELSD 80/85LT Sedere Sedex ELSD 90LT Sedere Sedex LC ERC RefractoMax 520 series OpenLAB CDS EZChrom Edition CDS version and Driver Version n/a ClarityChrom CDS version and Driver Version supported by Data Apex; CC Chromeleon CDS version Driver version Cm 6.8 (driver not developed by KNAUER) ChromGate CDS version and Driver Version PurityChrom n/a Version: n/a n/a n/a CG Driver 446 n/a n/a n/a n/a n/a supported, driver was developed by MayLab Analytical 13) supported, driver was developed by MayLab Analytical 13) supported, driver was developed by MayLab Analytical 13) n/a supported by DataApex; CC supported by DataApex; CC supported by DataApex; CC supported by DataApex; CC supported by DataApex; CC supported by DataApex; CC supported by DataApex; CC supported by DataApex; CC 6.1.0, RS-232 Antec Decade II n/a supported by DataApex; CC 5.x, RS-232 Antec Decade Elite n/a supported by DataApex;CC 5.X, USB, LAN Grace/Alltech ELSD supported by DataApex; CC 5.x, LAN - CG Driver 446 n/a - CG Driver 446 n/a - CG Driver 446 n/a - CG Driver 1466 n/a n/a (Cm 6.8) supported, driver was developed by MayLab Analytical 14) Version: n/a (Cm 6.8) supported, driver was developed by MayLab Analytical 14) Version: n/a (Cm 6.8) n/a Version: Supported in Cm 7.2 (driver not developed by KNAUER) n/a n/a n/a n/a n/a n/a n/a n/a - Separate, license-free driver upon request or from Grace webpage, driver was developed from Grace/Alltech 1) only LAN is supported 2) to set a static IP address a separate tool is required 3) via RS-232 the maximum data rate is limited to 10 Hz 4) in ClarityChrom 5.0.2, runs with data rates >10 Hz may not be stored with 3D data; recommended maximum data rate is 10 Hz, fixed in later versions 5) generic drivers do not support all of the devices features; only control via RS-232, data acquisition requires A/D converter; should also work with Chromeleon 7.x 6) a trigger on Start input also starts the internal wave length program 7) for control via RS-232 only baud rate is supported 8) device only supports data acquisition via RS-232, no wavelength control 9) LAN only via separate LAN adapter A4442; default communication adapter is PCI interface card for RS-422, PCI interface card requires a PCI slot on the computer; driver for PCI interface card only works with Windows NT/2000, Windows XP or Windows 7 32-bit. 10) RS-232 only, maximum data rate 0.5 Hz, A/D converter for higher data rates required; special RS-232 cable required (KNAUER part number G0743) - 64

71 Supported devices 11) RS-232 only, special firmware version 0.90 required, feature set of RF-10 AXL only (1 channel only, no flow cell temperature control, no scan), maximum data rate 5 Hz, A/D converter for higher data rates required; special RS-232 cable required (KNAUER part number G0743) 12) special RS-232 cable is required 13) driver must be installed separately and is protected with a separate USB dongle, detector cannot be used for fractionation; KNAUER driver part number A ) driver must be installed separately and is protected with a separate USB dongle, detector cannot be used for fractionation; KNAUER driver part number A ) 4 channels, up to 50 Hz data rate 16) 8 channels, up to 10 Hz; PurityChrom offers only limited 3D functionality, refer to the PurityChrom manual 17) up to 2 Hz, not in a system with CM 2.1S 18) not in a system with RID 2.1L Assistant (up to three devices in one housing) ASM 2.1L 1), 2) OpenLAB CDS EZChrom Edition CDS version and Driver Version Driver ) ClarityChrom CDS version and Driver Version CC 3.0.7; Driver ) 5) Chromeleon CDS version Driver version ChromGate CDS version and Driver Version PurityChrom n/a CG 3.3.2; Driver 2825 Version: ) 1) configuration depends on order; please refer the allowed configuration and restrictions in our FAQ 2) only one LAN port for all devices 3) only one valve as fraction collector, no cascading if controlled as ASM; devices can also be controlled as separate devices via IP port; if a valve configured as KNAUER MultiValve FC valve can be controlled as separate device via IP port, only then cascading is possible 4) ASM is only supported as complete Assistant; included modules cannot be controlled as separate devices (IP port separation is not supported) 5) only one valve as fraction collector, no cascading 6) ASM is not supported as device; the included modules can be controlled as separate devices via IP port; only one valve as fraction collector Autosamplers (one autosampler in a system) AZURA Autosampler AS 6.1L Smartline Autosampler ) PLATINblue Autosampler AS-1 Autosampler KNAUER Optimas Smartline Autosampler 3800 (also WellChrom K-3800, Spark Marathon) Smartline Autosampler 3900 (also Spark Midas) OpenLAB CDS EZChrom Edition CDS version and Driver Version Driver 2109 Driver 1017 Driver 1017 Driver 1017 ClarityChrom CDS version and Driver Version Chromeleon CDS version Driver version CC Cm 7.2 Regular Class 3 driver CC / LAN from CC Driver ) CC 6.1.0, Driver 5015 CC Driver ) Cm 7.2 Regular Class 3 driver; Cm 6.8 SR10 Regular Class 3 driver 6) n/a CC ) Cm 6.8; Generic driver 4) n/a CC ) Cm 6.8; Generic driver 4) Spark Triathlon n/a CC ) Cm 6.8; Generic driver 4) ChromGate CDS version and Driver Version PurityChrom n/a Version: CG Driver 1466 / LAN from CG Driver 1640 Cm 6.8 SR10 Regular Class 3 driver CG PLATINblue Edition 1751; Regular 2825 n/a n/a n/a n/a Version: ) CG Driver 1787 Version: CG 2.8 Version: CG 2.55 Version:

72 Supported devices Autosamplers (one autosampler in a system) Kontron Autosampler 360 Kontron Autosampler 46x Kontron Autosampler 56x OpenLAB CDS EZChrom Edition CDS version and Driver Version ClarityChrom CDS version and Driver Version Chromeleon CDS version Driver version ChromGate CDS version and Driver Version n/a CC ) - CG Driver ) n/a - - CG Driver ) n/a CC ) - CG Driver ) PurityChrom n/a n/a n/a 1) some device features have been added from time to time; therefore, not all of the current features are supported in the software from beginning (as LAN connection, TCP/IP support, 84+3 vial tray, 108 vial tray, thermostating up to 40 0C, 0.1 µl increments for injection volume and smallest injection volume); the features require a device with the appropriate firmware and hardware version 2) LAN default configuration is LAN via DHCP, static IP address requires access to built-in LAN module 3) driver was developed by Data Apex 4) upon request - driver was not developed by KNAUER 5) special RS-232 cable is required 6) no Stacked Injection supported Analog/Digital Converters KNAUER Interface Box IFU 2.1 LAN 2) KNAUER Interface Box IFU 2.1 3), 4), 5) KNAUER Interface Box IF2 4) Smartline Manager ) Smartline Manager ) KNAUER HPLC Box Model 96 10) Kontron Interface Card 14) OpenLAB CDS EZChrom Edition CDS version and Driver Version 1) Driver 2092 Driver 1017 Driver 1017 ClarityChrom CDS version and Driver Version CC Driver 5043 (compatible with CC/ CCP 5.x versions) CC Driver 3078, in former version as IF2 5) Chromeleon CDS version Driver version ChromGate CDS version and Driver Version PurityChrom n/a n/a Version: n/a CG Driver 2825, in former version as IF2 5) Version: ) CC / 2.6 7), 8) n/a CG 3.1 9) Version: n/a CC 2.4.4/2.6 7), 8) n/a CG 3.1 9) n/a Driver 1017 CC Driver ) n/a CG Driver ) n/a CC ) n/a CG ), 13) n/a n/a - - CG Driver 1466 n/a n/a 1) A/D converter in OpenLAB must be configured separately; menu is available via Start Programs Agilent Technologies OpenLAB CDS EZChrom Edition, right click on Agilent SS420X Interface Configuration and select Run as Administrator 2) to set a static IP address a 3rd party tool is required 3) built-in USB-to-serial converter requires separate installation of a driver in Windows operating system, can be found on the HPLC software CD or KNAUER webpage (Download section for IFU 2.1, Article Number AZB00); check Windows Device Manager for the COM port number of the converter, this number must be selected in the software configuration 4) as the required serial number in the configuration, enter the last 5 digits of the serial number on the label; if the first digit is a 0 (zero), only enter the last four digits 5) in previous software versions IFU 2.1 can be controlled as IF2 or Manager 5000 if IFU 2.1 is not listed in the module list 6) analog input only 7) in ClarityChrom version only data acquisition via analog input is supported (max. 10 Hz), analog out supported from ClarityChrom version 2.6 8) 50 Hz option for channel 1 supported from version ClarityChrom version 2.6 9) 50 Hz option for channel 1 supported from version ChromGate version ) as the required serial number in the configuration, enter the last 5 digits of the serial number on the label, if the serial number is higher than 40000, please enter only the last four digits 66

73 Supported devices 11) supported as Basic Edition, ClarityChrom will switch the box into the required mode 12) supported as Integration Box only for data acquisition via analog input; box must be switched into the correct mode with special software 13) 100 Hz option for channel 1 supported from version ChromGate version ) requires an ISA slot on the computer Thermostats/Column ovens Column Thermostat CT 2.1 PLATINblue Column Thermostat T-1 Smartline Column Oven / Column Thermostat JetStream / PLATINblue T-1 Basic 1), 2) Smartline Column Oven ) OpenLAB CDS EZChrom Edition CDS version and Driver Version Driver 1027 Driver 1017 Driver 1017 ClarityChrom CDS version and Driver Version Chromeleon CDS version Driver version CC driver 4102 Cm 7.2 Regular Class 3 driver CC Driver 4095 CC Cm 6.8 SR10 Regular Class 3 driver Cm 6.8 SR10 Regular Class 3 driver ChromGate CDS version and Driver Version n/a CG PLATINblue Edition 1751; Regular 2825 CG 3.1 n/a CC n/a CG Driver 1466 n/a 1) firmware version b1.17 or higher required 2) if connected, control panel must show Choose Function before the instrument will be opened 3) device does not support static IP address, only LAN via DHCP from firmware version 1.11 PurityChrom n/a n/a Coming soon Electrical valve drives AZURA Valve V 2.1S 1) WellChrom electric valve drives K-6, K-12, K-16 Smartline valve drives S6, S12, S16 1) VICI Valco drives OpenLAB CDS EZChrom Edition CDS version and Driver Version ClarityChrom CDS version and Driver Version Chromeleon CDS version Driver version ChromGate CDS version and Driver Version PurityChrom Driver 1017 CC Driver ) n/a 2), 3) n/a 1) Version: n/a 4) CC CM 6.8 Generic driver CG 2.8 Version: ) Driver 1017 Driver 1017 CC CC ) CM 6.8 Generic driver 3) Implemented (not by KNAUER) CG 3.1 1) Version: Generic Driver 3) - 1) can be controlled as KNAUER switching valve if not listed in the module list of previous software versions (maybe limited on RS-232) 2) generic driver for KNAUER switching valves will also work with AZURA V 2.1S (RS-232 only) 3) generic drivers do not support all of the devices features (e.g. RS-232 only) 4) can be controlled as KNAUER switching valve 5) driver was developed by Data Apex Other supported instruments OpenLAB CDS EZChrom Edition CDS version and Driver Version ClarityChrom CDS version and Driver Version Chromeleon CDS version Driver version ChromGate CDS version and Driver Version PurityChrom Airsensor 1) n/a n/a n/a n/a Version: Pressure control (pressure difference) 2) n/a n/a n/a n/a Version:

74 Supported devices 1) communication via game port adapter 2) requires KNAUER IFU 2.1/ IFU 2.1 LAN Fraction collectors (one fraction collector in a system, no combination of a fraction valve and a fraction collector possible) KNAUER MultiValve Fraction Collector (all KNAUER electric valve drives; 2, 6, 12 and 16 ports) ASM 2.1L valve Smartline Fraction Collector ) Büchi Fraction Collector C-684 5) Büchi Fraction Collector C-660 ISCO Fraction Collector Foxy Jr. ISCO Fraction Collector Foxy R1 6) ISCO Fraction Collector Foxy R2 6) ISCO Fraction Collector Foxy 200 9) Labocol Vario 2000 Fraction Collector Labocol Vario 4000 Fraction Collector VICI Valco MultiValve Fraction Collector Virtual Fraction Collector Injection Module (combined module with pump and injection valve for injection of large injection volumes, allows for stacked injection) 12) OpenLAB CDS EZChrom Edition CDS version and Driver Version Driver ) ClarityChrom CDS version and Driver Version Chromeleon CDS version Driver version ChromGate CDS version and Driver Version CCP 2.6 2) n/a CG Driver 423 1) PurityChrom Version: ) CC 3.0.7; Driver n/a CG 3.3.2; Driver 2825 Version: Driver 1017, 13) 3078, 14) 15) 16) n/a CCP 2.6 n/a CG Driver 1640 n/a n/a n/a n/a CG Driver 423 n/a Driver 1017 CCP 2.6 n/a CG Driver 423 n/a n/a CCP 2.6 Implemented (not by KNAUER) CCP Driver Implemented (not by Driver ) 1094 (RS-232 only) KNAUER) 8) Driver ) CCP Driver 1094 (RS-232 only) 8) n/a CCP 2.6 CCP Driver 1100, 9) Driver 1017 Driver 1017 Driver ) Driver 1017 Driver 1017 CG Driver 423 Version: CG3.3.2 Driver ) n/a CG3.3.2 Driver ) Implemented (not by KNAUER) CG Driver 1466 CG3.3.2 Driver 1751, 9) n/a n/a CG Driver 423 n/a n/a n/a CG Driver 1640 n/a n/a from KNAUER, valve is supported in ASM 2.1L as FC 10) 11) n/a CG Driver ) CCP 2.6 n/a CG Driver 423 n/a n/a n/a CG Driver 1640 n/a Version: Version: n/a Version: ) 1) up to 32 valves in a cascade 2) up to 12 valves in a cascade 3) only one valve can be used as fraction collector, no cascading is supported 4) switch between RS-232 and LAN with a switch on the devices rear panel 5) requires special relay module for serial communication 6) touch panel is not blocked in remote, do not touch it while the device is under software control 7) LAN control from firmware version , LAN control support in firmware versions and faulty 68

75 Supported devices 8) only RS-232, communication via LAN is not supported 9) support is discontinued due to several incompatible firmware versions; not all devices may work with the driver 10) not all available valve types can be built-in into ASM 2.1L 11) driver from DataApex available; does not support KNAUER fractionation features 12) several pumps were added to the module when released; however, a pump is only supported in the injection module if the mentioned pump is also available as a separate pump; in ASM2.1L pump and valve must be controlled separately, refer software manual for detailed information 13) only one valve as fraction collector, no cascading if controlled as ASM; if configured as KNAUER MultiValve FC valve can be controlled as separate device via IP port, only then cascading is possible 14) only one valve as fraction collector in ASM, no cascading 15) only one valve as fraction collector, no cascading if controlled as ASM; if configured as KNAUER MultiValve FC valve can be controlled as separate device via IP port, only then cascading is possible 16) only one valve as fraction collector, no cascading Mobile Control Mobile Control (KNAUER): Version also with data acquisition support, supports AZURA devices: - Pump P 2.1S/P 4.1S Firmware V Pump P 6.1L Firmware V Pump P 2.1L Firmware V Detector UVD2.1S Firmware V Detector UVD 2.1L Firmware V Detector MWD 2.1L Firmware V Detector DAD 2.1L Firmware V Detector DAD 6.1L Firmware V Detector RID 2.1L Firmware V Valve V 2.1S Firmware V Assistant ASM 2.1L Firmware V Column thermostat CT 2.1 Firmware V Autosampler AS 3950 Firmware V01.16 Mobile Control version requires OpenLAB EE from driver version 2092 and ClarityChrom /Prep 5.0.5/6.1.0 from driver version Mobile Control (KNAUER): Version also with data acquisition support, supports AZURA devices: - Pump 6.1L (from firmware version 1.03) - Pump 2.1L (from firmware version 1.08) - Pump P 2.1S/P 4.1S (from firmware version 1.32) - UVD 2.1L (from firmware version 2.04) - DAD 2.1L (from firmware version 1.1) - DAD 6.1L (from firmware version 1.21) - MWD 2.1L (from firmware version 1.1) - UVD 2.1S (from firmware version 1.08) - ASM 2.1L (from firmware version 1.11) - Valve V 2.1S (from firmware version 4.14) - CT 2.1 (from firmware version 1.06) 69

76 Supported devices Mobile Control (KNAUER): Version supports AZURA devices: - Pump 6.1L (from firmware version 1.02) - Pump 2.1L (from firmware version 1.07) - Pump P 2.1S/P 4.1S (from firmware version 1.32) - UVD 2.1L (from firmware version 2.04) - DAD 2.1L (from firmware version 1.1) - DAD 6.1L (from firmware version 1.21) - MWD 2.1L (from firmware version 1.1) - UVD 2.1S (from firmware version 1.08) - ASM 2.1L (from firmware version 1.10) - Valve V 2.1S (from firmware version 4.14) - CT 2.1 (from firmware version 1.06) Monitor Mode supported in OpenLAB EE from driver version 2047 and ClarityChrom/Prep 5.0.5/6.1.0 from driver version Other software LabView (National Instruments): Driver for AZURA Pumps P 2.1S / P 4.1S, Smartline 100, BlueShadow Pumps 10P / 20P HyStar TM (Bruker): Compass HyStar TM 3.2 Plug-in: Drivers for PLATINblue system (Pump P-1, DAD PDA-1, UV Detector MW-1, Column Thermostat T-1, Autosampler AS-1, Manager M-1 (LPG valves only, interface module is not supported) Compass HyStar TM 4 Plug-in: Driver for AZURA DAD 6.1L Xcalibur TM (Thermo): Version and 2.1: Driver for PLATINblue Pump P-1, UV Detector MW-1, DAD PDA-1, Autosampler AS-1, Column Thermostats T-1 and T-1 Basic and Smartline Pump S 1050, UV Detector S 2550, Autosampler S 3950 and Column Thermostat JetStream 70

77 Conversion tables Dimensions mm inches 0,10.004" 0,12.005" 0,15.006" 0,25.010" 0,40.016" 0,50.020" 0,75.030" 1,00.040" 1,50 060" 2,00.080" 4,60.180" 6,00.236" 6,40.253" 7,00.276" 10,00.400" inches mm 1/32" 0,8 1/16" 1,6 1/8" 3,2 1/4" 6,4 3/8" 9,5 1/2" 12,7 1" 25,4 Tubing volume/length conversion chart Tubing ID µl/cm µl/in.004" 0,08 0,21.005" " " " " " " " " " Pressure conversion chart MPa bar psi Temperature conversion chart C F C F C F

78 Notes 72

79 Order request Company: Name: Date: Part No.: Description Qty. Price 73

80 We separate molecules and unite people. As experts in liquid chromatography, we continue the path of our founder Dr. Knauer: Together with scientists in the lab we develop innovative and individual solutions. We are curious and creative. This makes us the ideal partner for those wanting more than a standard solution. KNAUER is an established partner in science. We support research and technological advancement today and in the future. Technical data or prices are subject to change without notice. Prices may vary by country and do not include taxes, customs duties or delivery. Distributed by: Please check out the KNAUER website for further detailed information: All trademarks are the property of their respective owners. Our general terms and conditions apply: Information on chemical compatibilities are stated in the user manuals: KNAUER 2018 V7854-2US/2.0/05.17/KN Version 1.1 KNAUER Wissenschaftliche Geräte GmbH Hegauer Weg Berlin, Germany (Fax) info@knauer.net