NEW products for 2012

Transcription

1 NEW products for 2012 Personal thermal cyclers Mid-sized thermal cyclers Full-size thermal cyclers Networkable thermal cycling Gradient cycling Interchangeable blocks Realtime PCR system Touch Interface Upgradable 4 year warranty Page 1

2 Welcome to Techne Equipment for the Life Sciences Techne is a long established name and world leader in the manufacture of temperature control and science equipment essential for the life sciences, research, clinical and general laboratory applications. In this edition we are proud to introduce the new Prime thermal cycler range and new PrimeQ real-time PCR system: The Prime instrument delivers the most feature packed personal cycler on the market. It shares the same touch interface as the full sized models as well as fast ramp rate and excellent uniformity. The Prime instrument is a full sized model that will become your reliable laboratory partner for routine PCR. The basic unit can be upgraded to achieve gradient capability without a hardware change. This allows you to better manage your budget by adapting your expenditure to changing laboratory needs. The Prime Elite is our top of the range model offering enhanced features and instrument network connectivity o Fastest ramp rates o Gradient capability as standard o Unique CD style drawer mechanism for sample loading o Stackable design to save valuable laboratory space o Server and satellite models to enable network connectivity The PrimeQ real-time PCR system has been designed with an open architecture and chemistry format that allows user flexibility in qpcr methods and research pursuits. The supporting Quansoft software provides an easy to use comprehensive data analysis package with the flexibility to edit experiment setup even after the run is complete. Techne products are designed, manufactured and marketed in an ISO9001:2008 environment. Every step from new product development to after sales service follows documented and traceable procedures. The result is a high standard of service and qualityfocused culture committed to total customer satisfaction. At Bibby Scientific HQ, qualified scientists and electrical engineers are available to provide technical support and application advice (see Technical Information section for contact details). All electrical products produced by Bibby Scientific conform to the latest safety directives including the European CE requirements. For total compliance, all products are tested and approved by a fully accredited external test house. Bibby Scientific is also compliant with the Waste Electrical and Electronic Equipment directive, WEEE and the Restriction of the use of Certain Hazardous Substances, RoHS. You can find copies of our ISO certificates on All the products featured in this catalogue are available through a wide range of national and international distributors. For your local distributor details and up-to-date product information please visit our Techne website at Here you will also find software downloads, as well as information on news and events, exhibitions and seminars, frequently asked questions, and much more. The Techne brand is committed to providing the very best life science and temperature control equipment as well as the highest level of service, before, during and after the sale. Page 2

3 Thermal Cyclers Introducing the New Prime thermal cycler range Techne have upgraded our established thermal cycler range to provide easier programming and increased instrument flexibility. The Prime range has been designed to enhance the user experience with a touch interface and identical software for all models. The new range provides high performance, exceptional value and a reliability backed by the market leading 4 year warranty. Page 4 Page 6 Page 8 Page 10 Page 12 Page 1 Selection Table Personal and Mid-size Cyclers Full Size Cyclers Networkable Thermal Cyclers Workbench Software PrimeQ Real-Time PCR System Find out more! Please scan the QR/Mobile Tag with your smartphone for more information Page

4 Thermal Cycling at a Glance Techne have upgraded our established thermal cycler range to provide easier programming and increased instrument flexibility. The Prime range has been designed to enhance the user experience with an outstanding touch interface and identical software for all models. The new range provides high performance, exceptional value and a reliability backed by the market leading 4 year warranty. Personal Cycler Mid-size Cycler Model Prime PrimeX PrimeG Block formats 24x 0.2ml 48x 0.2ml 48x 0.2ml 18x 0.5ml 0x 0.5ml 0x 0.5ml Interchangeable blocks Maximum ramp rate.0 C.0 C.0 C Temperature accuracy* <±0.25 C <±0.25 C <±0.25 C Temperature uniformity* <±0. C <±0. C <±0. C Program Interface.5 VGA.5 VGA.5 VGA touch screen touch screen touch screen Stored programs Networking capability Adjustable heated lid Fixed Gradient Range - Can be upgraded 0-80 C to PrimeG spec Max Gradient C Dimensions (w x d x h), mm 210 x 50 x x 50 x x 50 x 180 Licensed for PCR Warranty 4 years or 4 years or 4 years or 80,000 cycles 80,000 cycles 80,000 cycles * Recorded at 55ºC for Prime and Prime and 50ºC for Prime Elite Follow Techne equipment on Twitter! For more information please visit Page 4

5 Full size Cycler Network Connectivity Prime PrimeG Prime Elite Prime Elite Satellite 96x 0.2ml 96x 0.2ml 96x 0.2ml 96x 0.2ml 60x 0.5ml 60x 0.5ml 60x 0.5ml 60x 0.5ml 84 well plate 84 well plate 84 well plate 84 well plate.4 C.4 C 5.0 C 5.0 C <±0.25 C <±0.25 C <±0.2 C <±0.2 C <±0.2 C <±0. C <±0. C <±0. C 5.7 VGA 5.7 VGA 5.7 VGA Programmable via Prime Elite touch screen touch screen touch screen or PC Workbench software Automatic Automatic Can be upgraded 0-80 C C C to PrimeG spec - 29 C (19ºC for 84) 0 C 0 C 240 x 420 x x 420 x x 75 x x 75 x 12 4 years or 4 years or 4 years or 4 years or 80,000 cycles 80,000 cycles 100,000 cycles 100,000 cycles Techne videos now on Youtube! For more information please visit Page 5

6 Prime Personal Thermal Cyclers Techne have upgraded their established personal cycler range to provide easier programming and increased instrument flexibility, whilst maintaining the rapid ramping rates and small footprint that make them ideal for research and teaching laboratories. Using a new colour touchscreen and ready-to-go templates the user is intuitively guided to create even the most complicated programs. The new interface delivers a standardised user experience across the entire Techne thermal cycler range. Gradient cycling can be achieved directly using the PrimeG instrument or by upgrading the PrimeX unit. This enables the laboratory to manage changing cycling requirements in a cost effective manner. The Techne Prime cycler range is the most reliable low cost personal cycler solution on the market and is backed up by a 4 year warranty. Key features Colour touchscreen for fast program setup Small space-saving footprint Temperature range 4 C to 100 C Fast ramp rate of up to.0ºc/sec Block options; 24 x 0.2ml (compatible with 8 well microtube strips) and 18 x 0.5ml Data transfer via USB Backed by Techne s 4 year warranty PrimeX Mid-size Thermal Cyclers Prime The PrimeX delivers all the features of the Prime instrument but with an expanded sample capacity, accommodating 48 x 0.2ml microtubes, 0 x 0.5ml tubes plus half a 96 well plate in a horizontal format. Design flexibility allows the unit to be upgraded to included gradient cycling capability. Additional Features to Prime Larger block sizes; 48 x 0.2ml, 0 x 0.5ml and half a 96 well plate in horizontal format. Upgradable for gradient cycling (for specification see PrimeG) PrimeG Mid-size Thermal Cyclers The PrimeG is a small gradient thermal cycler that builds on all the features of the PrimeX instrument. The 48 well block format offers eight columns for annealing temperature optimisation and six rows for optimising reagents such as MgCl 2 and primer concentrations. Annealing temperatures can be optimised over 14ºC between temperatures 0 C to 80 C. The gradient calculator function displays the temperature for each of the eight columns, ensuring easy replication of thermal conditions. Additional Features to PrimeX Gradient can be applied across a temperature range of 0 C to 80 C Maximum Gradient 14 C Gradient calculator function Purchase of this instrument conveys a limited, non-transferable immunity from suit for the purchaser s own internal research and development and applied fields other than human in vitro diagnostics under non-real-time thermal cycler patents of Applied Biosystems LLC. Page 6

7 Prime Thermal Cyclers Technical Specification Prime PrimeX and PrimeG Sample capacity: 0.2ml Sample capacity: 0.5ml 18 0 Maximum heating rate.0 C/s.0 C/s Block temperature range 4 C to 100 C 4 C to 100 C Block uniformity at 55 C <±0. C <±0. C Temperature accuracy at 55 C <±0.25 C <±0.25 C Gradient is standard on PrimeG and can be upgraded on the PrimeX Gradient Range - 0 C to 80 C Maximum Gradient - 14 C Minimum Gradient - 1 C Gradient calculator - Type 4 peltier element/block 4 peltier element/block Selectable heated lid temperature 100 C to 115 C or off 100 C to 115 C or off Heated Lid pressure Fixed Adjustable, dependent on consumables Program interface.5 VGA colour touchscreen.5 VGA colour touchscreen with graphical display graphical display Maximum number of programs stored Data transfer USB port USB port Auto re-start on power failure Yes Yes Dimensions (w x d xh), mm 210 x 50 x x 50 x 180 Weight, kg 6 6 Voltage V, 50-60Hz V, 50-60Hz Power 155W 155W 4 year warranty for the Prime thermal cycler and 80,000 cycles or 4 year warranty for the thermal block, whichever comes first. Ordering Information Part Code PRIMEBASE/02 PRIMEBASE/05 PRIMEX/02 PRIMEX/05 PRIMEX/USB PRIMEG/02 PRIMEG/05 Description Prime thermal cycler, 24 x 0.2ml Prime thermal cycler, 18 x 0.5ml PrimeX thermal cycler, 48 x 0.2ml PrimeX thermal cycler, 0 x 0.5ml PrimeX upgrade PrimeG gradient thermal cycler, 48 x 0.2ml PrimeG gradient thermal cycler, 0 x 0.5ml Replacement blocks Exchange of thermal cycler blocks must be carried out by a trained service engineer. Ordering Information Part Code PRIMEBASE/02/B PRIMEBASE/05/B PRIMEX/02/B PRIMEX/05/B Description Prime block, 24 x 0.2ml Prime block, 18 x 0.5ml PrimeX block, 48 x 0.2ml PrimeX block, 0 x 0.5ml Page 7

8 Prime Full Size Thermal Cyclers This full sized thermal cycler delivers both high performance and high throughput to provide maximum flexibility when processing a large number of samples in parallel. A versatile, fully interchangeable block system allows exchange in a matter of seconds without the need of tools (60 x 0.5ml, 96 x 0.2ml and 84 well plate formats available) User friendly programming is achieved via a colour touchscreen and intuitive software that is standardised across the entire Techne thermal cycler range. A USB port enables the transfer of programmes between instruments and temperature logs to your PC. A real time graphical display provides an instant visualisation of the program status. Key features Colour touchscreen for fast program setup. Versatile block format Temperature range 4 C to 100 C Fast ramp rate of up to.4ºc/sec Can be upgraded for gradient cycling Data transfer via USB Backed by Techne s 4 year warranty. Prime Gradient cycling can be achieved directly using the PrimeG instrument or by upgrading the Prime unit. This enables the laboratory to manage changing cycling requirements in a cost effective manner. The Techne Prime cycler range is one of the most affordable full sized thermal cyclers on the market and is backed up by a 4 year warranty. PrimeG Full Size Gradient Cycler The PrimeG is a gradient enabled thermal cycler with all the features of the Prime unit. The wide linear gradient with a range of 0 C allows annealing temperatures to be optimised in one experiment. The gradient calculator function displays the temperature for each of the eight columns, ensuring easy replication of thermal conditions. Additional Features to Prime Gradient can be applied across a temperature range 0 C to 80 C Maximum Gradient 29 C Gradient calculator function Purchase of this instrument conveys a limited, non-transferable immunity from suit for the purchaser s own internal research and development and applied fields other than human in vitro diagnostics under non-real-time thermal cycler patents of Applied Biosystems LLC. Page 8

9 Prime Full Size Thermal Cyclers Technical Specification Prime PrimeG Sample capacity: 0.2ml Sample capacity: 0.5ml Sample capacity: 84 well Yes Yes Maximum heating rate.4 C/s.4 C/s Block temperature range 4 C to 100 C 4 C to 100 C Block uniformity at 55 C <±0. C <±0. C Temperature accuracy at 55 C <±0.2 C <±0.2 C Gradient Can be gradient enabled Can be gradient enabled Maximum Gradient - 29 C (19 C for 84) Minimum Gradient - 1 C Type 8 peltier element/block 8 peltier element/block Selectable heated lid temperature 100 C to 115 C or off 100 C to 115 C or off Heated lid pressure Adjustable, dependent on Adjustable, dependent on consumables consumables Program interface 5.7 VGA colour touchscreen 5.7 VGA colour touchscreen Maximum number of programs stored Data transfer USB port USB port Auto re-start on power failure Yes Yes Dimensions (L x W x H), mm 420 x 240 x x 240 x 240 Weight, kg Voltage V, 50-60Hz V, 50-60Hz Power 450W 450W 4 year warranty for the Prime thermal cycler and 80,000 cycles or 4 year warranty for the thermal block, whichever comes first. Ordering Information Part Code Prime/02 Prime/05 Prime/84 PrimeX/USB PrimeG/02 PrimeG/05 PrimeG/84 Description Prime thermal cycler, 96 x 0.2ml Prime thermal cycler, 60 x 0.5ml Prime thermal cycler, 84 well plates Prime gradient upgrade PrimeG gradient thermal cycler, 96 x 0.2ml PrimeG gradient thermal cycler, 60 x 0.5ml PrimeG gradient thermal cycler, 84 wells Replacement blocks This versatile, fully interchangeable block system allows exchange in a matter of seconds without the need of tools. Ordering Information Part Code Prime/02/B Prime/05/B Prime/84/B Description Prime block, 96 x 0.2ml Prime block, 60 x 0.5ml Prime block, 84 well plates Page 9

10 Prime Elite Networkable Thermal Cyclers This premier cycler combines a high performance thermal engine with unique instrument design to deliver the smallest 4 block PCR networking solution on the market. Faster ramp rates and excellent block uniformity are achieved using the TERS Thermal Energy Recovery System. TERS harnesses the heat released during the cooling phase and delivers it back to the block during the next heating phase increasing ramp rates for faster cycling. Watch your network grow Individually... Add a Satellite... Add some more... Specifications Sample capacity: 0.2ml 96 Sample capacity: 0.5ml 60 Sample capacity: 84-well Yes Block temperature range 0 to 100 C* Block uniformity at 50ºC <±0. C Temperature accuracy at 50ºC <±0.2 C Gradient range 0 to 100 C* Maximum gradient 0 C Minimum gradient 1 C Gradient calculator Yes Pre-run sample cooling Yes at 4 C Maximum heating rate 5 C/s Selectable heated lid temperature 5ºC to 115 C or off Over-temperature cut-out Yes Regulated heated lid pressure Automatic Program interface 5.7 VGA colour touch screen graphical display Maximum number of programs 1000 stored Password protection Yes Auto re-start on power failure Yes Connection to PC control software Yes Tm primer calculator Yes Dimensions (w x d x h), mm 276 x 75 x 12 Weight, kg 14 Voltage V, 50-60Hz Power 950W *Minimum temperature is 0ºC or 20ºC below ambient; whichever is higher. 4 year warranty for the Prime Elite thermal cycler and 100,000 cycles or 4 year warranty for the thermal block, whichever comes first. Page 10

11 Space Saving Design Increasing your network requires valuable laboratory space. The unique Prime Elite design features enable a 4 block footprint of only 0.21m 2 Unique front loading sample draw o Easy access to interchangeable blocks o Heated lid engages on closure o Spring mechanism prevents over tightening Stackable design o Foot recesses hold instrument stable Front to back airflow o Enables side by side instrument placement Power button on front of instrument o No need to reach around instrument Prime Elite Networkable Thermal Cyclers Expand your PCR capacity as your throughput requirements grow. Use a Prime Elite server as a stand-alone instrument A Prime Elite server automatically detects a satellites on connection Each Prime Elite instrument can directly control Satellites or 9 via a USB hub Up to 9 Satellites can also be run from a PC via the Workbench software Complete your network Ordering information Description Prime Elite thermal cycler, 96 x 0.2ml Prime Elite thermal cycler, 60 x 0.5ml Prime Elite thermal cycler, 84 well Prime Elite satellite thermal cycler, 96 x 0.2ml Prime Elite satellite thermal cycler, 60 x 0.5ml Prime Elite satellite thermal cycler, 84 well Product Code ELITE/02 ELITE/05 ELITE/84 ELITESAT/02 ELITESAT/05 ELITESAT/84 Interchangable blocks Blocks are easily exchanged by removing six screws. Description Prime Elite block, 96 x 0.2ml Prime Elite block, 60 x 0.5ml Prime Elite block, 84 well Product Code ELITE/02/B ELITE/05/B ELITE/84/B Purchase of this instrument conveys a limited, non-transferable immunity from suit for the purchaser s own internal research and development and applied fields other than human in vitro diagnostics under non-real-time thermal cycler patents of Applied Biosystems LLC. Page 11

12 Techne Workbench Thermal Cycler PC Software A welcome addition to the Techne instrument range, the PC application Techne Workbench is the desktop version of the Techne touch interface. Carrying the same look and feel, Techne Workbench is your one-stop place for all your PCR programs on your computer. Key features Create, edit and manage programs on your PC Import and work with programs created on any Techne thermal cycler, and then export them back. View and organise temperature logs created on your Techne thermal cycler Use Workbench to directly control Prime Elite Satellites without the need for a Prime Elite base unit Page 12

13 Real-time PCR System Introducing the New PrimeQ In 1987 Techne produced our first thermal cycler. Since that time we have developed a high level of expertise in this field. We are now proud to launch our latest instrument to complement the Prime thermal cycler range. The PrimeQ Real-Time PCR instrument provides an open architecture and chemistry format that has been designed with user flexibility in mind. The Quansoft software package allows manipulation of raw data and experimental set up even after the run is complete. Page 14 PrimeQ Real-Time PCR System Find out more! Please scan the QR/Mobile Tag with your smartphone for more information Page 1

14 PrimeQ Real-time PCR System The PrimeQ instrument has been designed with the advantage of having an open architecture and chemistry format that allows the end user full flexibility of the qpcr methods and research they wish to pursue. Single White LED Light Source Ensures consistent power output to each well compared to tungsten halogen lamps or other multiple LED systems. Provides a long life span unlike tungsten halogen lamps that require frequent replacement. PMT (Photomultiplier tube) Detector PMT is designed for accurate detection of photons compared to CCD cameras which are just an imaging system. PMT provides raw data for analysis that can be manipulated directly whereas CCD uses image comparisons interpreted by internal algorithms. Flexible scanning mechanism Allows both partial and full plate reads. High accuracy ensures no cross talk between well reads. Full plate read in 20 seconds per filter. Multiplex capabilities 4 paired excitation and emission filters housed in an individual cartridge system. Open system to preferred chemistry and not locked in to factory calibrated dyes. PrimeQ Range of excitation/emission wavelengths nm Photon Counting PMT Emission filter Dichroic mirror White light LED Excitation filter Fiber optic bundle = Excitation light = Emitted light Excitation light to the sample Emitted light collected from the sample Page 14

15 Introducing Quansoft Accompanying PrimeQ is our unique, intuitive software Quansoft. Employing four user-friendly editor functions Quansoft enables any real-time experiment to be created and analysed with ease. 4. Results editor Choose your analysis method and manipulate your raw data before packaging the results in a customised report. 1. Plate layout editor Define your PCR plate in seconds with colour coded sample identifications. Crossing line Noise threshold 2. Program editor Individual cycles and steps as well as a ramp read can be added quickly to build up and display the thermal program and read points. Quantification analysis Using intercalating dyes or probes PrimeQ provides a wide dynamic range and high sensitivity Linearity of at least 9 orders of magnitude. Detecting down to a single copy template or to achieve absolute quantification of 1nM fluorescein in a volume of 20µl.. Experiment editor Combine thermal cycling programs, plate layouts and analysis methods to generate a new complete experiment. Dissociation analysis Provides the user confident reporting in genotyping experiments and in product verification analysis. End point analysis Plus/minus scoring. Allelic discrimination. Page 15

16 PrimeQ Real-time PCR System Technical Specification Block format 96 x 0.2ml Block specification 8 x Peltier block employing quad-circuit technology to enhance performance Block uniformity at 50 :C < ±0. C Maximum ramp rate Up to 2.2 C Temperature range 4ºC to 98 C Sample volume 15 to 50ul Heated lid Adjustable between 100 and 115 C in 1 C increments, or off Maximum sample evaporation Less than 15% volume loss from any well using the plate sealing recommended by Techne PC connectivity USB, OS Win XP SP or higher and Win 7 (one unit per PC) Plate format Low profile 96-well plate (skirted or non-skirted) Excitation source Solid state white light source Detector Photon counting photomultiplier tube Multiplex dye detection Up to 4 dyes per reaction tube User selected filters Maximum of 4 paired excitation/emission filter cartridge systems suitable with currently used dyes* Fluorescence excitation range nm (standard filters are centred at 485, 50, 580 and 640nm) Fluorescence detection range nm (standard filters are centred at 520, 560, 615 and 685nm) Dynamic range At least 9 orders of magnitude of target DNA concentration Sensitivity (detection threshold) 1nM fluorescein in a 20ul sample * Custom filter cartridge systems available upon request Ordering Information Part Code PRIMEQ FC01 FC02 FC0 FC04 FC05 Description PrimeQ real-time PCR System (supplied with filters FC02, FC0, FC04, FC05) FAM multiplex, 460nm excitation wavelength, 500nm emission wavelength Green, FAM/SYBR, 485nm excitation wavelength, 520nm emission wavelength Yellow, HEX, 50nm excitation wavelength, 560nm emission wavelength Red, ROX 580nm excitation wavelength, 615nm emission wavelength Blue, Cy5, 640nm excitation wavelength, 685nm emission wavelength Page 16

17 Four Brands One Name Hundreds of Products Bibby Scientific Ltd is one of the largest broad based manufacturers of laboratory products worldwide. Bibby Scientific Ltd provides internationally recognised brands with reputations for product quality and exceptional performance. These famous brands are now brought together in a single package to offer an excellent level of quality, service and support. Stuart Benchtop Science Equipment Jenway Equipment for Analysis Techne Equipment for the Life Sciences Electrothermal Equipment for Cooling, Stirring and Heating Find out more!!! Please scan the QR/Mobile Tag with your smartphone for more information Page 17

18 Contact Bibby Scientific - UK (Group HQ) Beacon Road, Stone, Staffordshire, ST15 0SA, United Kingdom T: +44 (0) F: +44 (0) E: sales@bibby-scientific.com Bibby Scientific France SAS Bâtiment le Deltaparc PN II, 7 rue du canal BP 5547 Villepinte, ROISSY Cdg T: 00 (0) E: bsf@bibby-scientific.fr Bibby Scientific Italia S.r.l. Via Alcide De Gasperi n. 56, Riozzo di Cerro al Lambro (MI) T: F: E: marketing@bibby-scientific.it Bibby Scientific - US Terri Lane, Suite 10, Burlington, NJ 08016, USA T: F: E: labproducts@techneusa.com Bibby Scientific - Middle East PO Box Engomi 24 Nicosia, Cyprus T: F: E: sales@bibby-scientific.me Bibby Scientific Singapore Prudential Tower, Level 26, 0 Cecil Street, Singapore T: F: +44 (0) E: sales@bibby-scientific.com Find out more!!! Please scan the QR/Mobile Tag with your smartphone for more information w w w. b i b b y - s c i e n t i f i c. c o m