NanoPrint Enterprise Level LM60 and LM210 Microarray Printers. Users Manual v
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1 Enterprise Level LM60 and LM210 Microarray Printers Users Manual v
2 2
3 Table of Contents Introduction page 4 System Overview page 5 Software Features page 7 System Options and Advantages page 8 System Specifications and Features page 9 System Installation and Performance page 14 Ordering Information page 22 3
4 Introduction microarrayer printers are enterprise level linear drive systems that provide universal platforms for research and diagnostic microarray manufacturing. Based on advanced nanotechnology, Arrayit robots move with 500 nanometer resolution and deliver sub-nanoliter sample volumes using high throughput, patented contact printing devices. Arrayit printing devices are covered by U.S. Patent 6,101,946 and these devices require a license for diagnostic applications. Please contact us for licensing information regarding diagnostic microarray manufacturing. 4
5 System Overview Figure 1. Shown is the LM60 system with a capacity of three 384-well sample microplates printing onto 60 standard glass slide substrates. Worktables for printing into microplates, plate size glass and other micro-fluidic biochip formats are available. Figure 2. Shown is the LM210 system with a capacity of three 384-well sample microplates printing onto 210 standard glass slide substrates. 5
6 Microarrayers are industrial-level platforms for all microarray manufacturing applications including DNA microarrays, protein microarrays, and other types of biomolecules. systems enable the manufacture of microarrays utilizing ArrayIt Professional, 946 and Stealth Style Micro Spotting Pins. The uses superior linear drive motion control technology and proprietary Warp1 controllers, and is compatible with all standard microarray surfaces made by ArrayIt and other glass substrate slide open platform vendors. Systems are easily configured to print onto different types of surfaces including 96 and 384-well plates, plate-sized glass, and proprietary cartridges and cassettes by taking advantage of flexible and modular deck configurations and an easy to use software interface. The Microarray Manager Software combines unparalleled power and simplicity in a graphical user interface. Click here. 6
7 Software Features A Method Creation Wizard User & version control management Custom calibration of the slide and microplate positions Complete sample tracking Support of input and output data files Custom microarray designs Customizable speed profiles and wash protocols Automatic Method Validation Runtime sample and spotting views as well as a Simulation Mode Easy to use graphical Re-Print Wizard The high speed, high precision linear servo control system of the produces superior instrument performance that is essentially free of friction, noise and thermal emission. Combined with the compact bench top design, user configurable worktable, humidity and particulate control, a host of available options, and the flexible and sophisticated software, systems offer the complete solution to high performance microarray printing. 7
8 System Options and Advantages 192 Pin Printhead (48 Pin Professional Printhead provided as standard) Anti-vibration table Peltier-driven microplate cooling Microplate printing worktables Custom worktables for proprietary printing cassettes, biochips and cartridges Worktable conveyor Microplate stackers, refrigerated with lid removal and barcode reader Dehumidification Extended service warranty The value of the linear servo motor technology used on Microarrayers: Nanometer scale positional accuracy Quiet operation No vibration No heat generation No motor dust Superior Accuracy Near maintenance-free operation High speed High load capacity Zero "sagging" with loss of power Safer and much more durable than stepper motors 8
9 System Specifications and Features Software Interface Intuitive and Graphic Windows 2000 or Windows XP User Interface User Management Password protected access levels for User, Manager and Administrator Data Files Both import and export of data files compatible with all current microarray detection instruments Version Control Complete tracking of all methods, version and backups Automatic Method Validation Automatically validates for compatible method parameters Sample and printing Views Each available in real-time during operation Method Creation Wizard Only minimal information required to create microarray printing protocols Simulation Mode Pre-test print methods in software prior to running programs Flexible Microarray design, pin configuration, worktable layout, source and destination type, offsets, speed profiles, wash/dry parameters and pre-print locations Hardware Size (L x W x H, cm) LM60 (110 x 85 x 56 cm), LM210 (164 x 85 x 56 cm) Weight (kilograms) LM60 (150 kg), LM210 (200 kg) Positional resolution 500 nanometers using linear drive Warp1 control Printing speed 48 spots per second or 192 Spots second using 192 Series Pins and Printheads. Destination Microarray substrates, glass slides, microplates, custom Printing technology Arrayit Pro, 946 or Stealth pins and printheads Number of pins Configurable 1 to 48 at 4.5 mm centers or 1 to 192 at 2.25mm Spot diameter 65 microns or larger to meet all applications Minimum spot spacing 50 microns Pre-printing User definable Wash/Dry station Ultrasonic with 2 wash positions and a dry station Number of microplates Three standard 384-well, customizable Number of substrates 60 substrate slides (LM60) or 210 substrate slides (LM210) Number of microplates well microplates (LM60) or well microplates (LM210) Microplate type 96-well or 384-well microplates Microplate cooling Cool 1-3 microplates, for protein microarray applications, or use the refrigerated stacker for higher throughput and hands free automation. Environmental control Fully enclosed, HEPA filtration and user-defined humidity control 9
10 Flexible Deck Configurations: Front left view of the showing three microplates, the wash/dry module and the printhead and pins printing onto glass substrates. A 48- pin NanoPrint printhead loaded with Micro Spotting Pins is printing location 8 on the deck. The modular deck design and platen cassettes allow users to easily switch between glass substrates, microplates, wafers and other types of substrates. Accessory Control Module (ACM): The ACM unit provides computer control for the wash/dry, humidity, and ultrasonication stations on the deck of the. The ACM unit fits neatly beneath the laboratory bench or under the optional anti-vibration table available with the system. The ACM is extremely quiet and operates only as needed by the system. Humidity is maintained in a user-specified manner of ±1%. Wash buffers are stored in carboys in front of the ACM. Pin Cleaning Module: microarrayers are each equipped with a station that provides pin washing, drying and sonication of 1-48 pins. The sonicator is automatically filled and emptied during the print run in a completely automated manner. Systems sensors prevent splashing and overflowing for pin and deck safety. Drying is accomplished by vacuum using a quiet but powerful ACM-controlled function. The Pin Cleaning Module is rugged, durable and 10
11 easy to maintain. ACM Connectivity: Easy connectivity between the ACM and the robot and accurate sensing of the humidity inside the chamber assures that proper humidity levels are achieved and maintained during the entire duration of each print run. Dehumidification is an option available at an extra cost and is highly recommended for users who wish to operate their systems in humid environments. The humidification and dehumidification systems are triggered by sensors that automatically maintain the levels set by the user. 11
12 Humidity Sensing: Accurate sensing of the humidity inside the chamber assures that proper humidity levels are achieved and maintained during the entire duration of each print run. Dehumidification is an option available at an extra cost and is highly recommended for users who wish to operate their systems in humid environments. The humidification and dehumidification systems are triggered by the RH sensor that automatically maintain the levels set by the user. Microplate Peltier Cooling: systems offer sophisticated microplate cooling via Peltier as an affordable and highly recommended option for users wishing to minimize sample evaporation during printing. Microplate cooling is also highly recommended for protein microarray applications to minimize protein denaturation and microbial growth in recombinant protein samples. The Peltier module fits directly beneath the microplate for highly efficient cooling while maintaining a low deck profile. Software: The extraordinary and highly intuitive graphical user interface provides users with software writing speed, power and flexibility. Shown here is a screen shot of the interstitial printing software command that automatically directs the robot to deposit the printed spots into a orange packing configuration for dramatically increased microarray printing density. 12
13 48 and 192 Pin Configurations are now available. 192 Pin configuration allows the user to run from 1 to 192 pins at 2.25 mm center to center spacing to pickup samples from 1536 well plates. Standard deliverable is 48 Pins at 4.5mm centers for use with 384 well plates (96 well plates can also be configured). NanoPrint software and unique Pin and Printhead design allows the end user to setup the pin tool any way that they like. Multiplex array processing systems, plates, biochips micro fluidics, and cartridges can all be easily configured. Superior Z-Axis Resolution for Optimum Spot Morphology: The Z-axis encoder on the reads at 250- nanometer resolution. Unlike belt and stepper motor driven Z-axis motion control, the offers highly precise resolution, repeatability and computer control over the speed and acceleration settings to ensure optimal printing onto any surface using our patented contact printing technology. Optimal parameters are set at the factory but can be easily changed by the user for printing onto many different surfaces. 13
14 System Installation and Performance Figure 3. The deck is configured in a module manner, allowing different worktables to be inserted and removed from the deck for different printing applications such as glass substrates, microplates, and proprietary cassettes and cartridges. The screenshot shows a worktable allowing printing into 15 microplates (96-well) for the LM60. 14
15 Figure 4. Data showing the printing consistency of eight different SMP3B pins in a single print run using the NanoPrint LM60. The data were provided courtesy of W.G. Shreffler and L. Bardina at the Mount Sinai School of Medicine (New York, USA). 15
16 Figure 5. Shown is the LM210 system configured in an ArrayIt class 100 cleanroom. The entire deck is accessible via two doors that open and close independently for user convenience and safety. The top of the Accessory Control Module (ACM) is shown beneath the robot. 16
17 Figure 6. Shown is the LM210 system configured in an ArrayIt class 100 cleanroom. Microarray manufacturing parameters including the number of pins, substrates, spots, technical replicates, printing pattern, worktable, printing offsets, speed, acceleration, and many other technical aspects are completely controllable by the operator via an intuitive graphical user interface (GUI). The MicroarrayManager Software allows users to write completely customized printing routines in minutes. Worktables allow the user to switch between glass substrate slides and microplates using different worktables and software routines. 17
18 Figure 7. Shown is the 48-pin printhead designed to hold up to 48 microarray printing pins of the Professional, 946 or Stealth series. The attachment bayonet allows the printhead to be attached and removed for cleaning easily and precisely, eliminating the need for system re-calibration. 18
19 Figure 8. Shown is the point of a Professional Series 3 (Pro3) microarray printing pin. Samples load into the pin uptake channel by capillary action, and direct contact between the sample layer on the pin tip and the glass printing substrate results in the formation of a printed microarray spot. The LM60 and LM210 systems control pin movements in 500 nanometer increments for unsurpassed printing precision at speeds of 48 spots per second. A total of 48 microarray printing pins of any tip size from the Professional, 946 or Stealth series may be used on our systems. 19
20 Figure 9. Shown is the Worktable Workstation for the LM60 and LM210 systems. The Worktable Workstation allows fully automated conveyor-based loading and unloading of glass slide substrate, microplate, and custom worktables from the deck. With a capacity of 60 worktables, the WorkTable Workstation allows fully automated printing onto 1800 glass substrates, well microplates, or 900 custom cassettes by special purchase quoted separately from standard NanoPrint configurations. 20
21 Figure 10. Shown here is 24 microarrays per slide substrate, a popular format easily setup and executed using the. This format is compatible with the AHC4x24, AHC1x24, and RC1x24 hybridization reaction tools. Using this format and an LM210 Microarrayer enables the automated manufacturing of more than 5,000+ microarrays per run and 1,400+ using a LM
22 Ordering Information Product Description Catalog ID LM60 Microarrayer LM210 Microarrayer LM60 Extended Warranty LM210 Extended Warranty Pro3 Printing Pins 946MP3 Printing Pins Printhead Worktable Workstation Microarrayer LM60 enterprise level industrial microarray manufacturing system with linear drives and WARP1 controllers on all three axes for sub-micron positional control. Each system is equipped with Arrayit s patented contact printing technology, full environmental control and highly intuitive software GUI. The standard LM60 worktable prints from 384-well microplates using 48 pins onto 60 glass substrates. Microarrayer LM210 enterprise level industrial microarray manufacturing system with linear drives and WARP1 controllers on all three axes for sub-micron positional control. Each system is equipped with Arrayit s patented contact printing technology, full environmental control and highly intuitive software GUI. The standard LM60 deck prints from three 384-well microplates using 48 pins onto 210 glass substrates. LM60 microarrayers include a one year warranty upon purchase. Users may purchase a comprehensive Extended Warranty (excluding pins and printheads) in one-year increments. LM210 microarrayers include a one year warranty upon purchase. Users may purchase a comprehensive Extended Warranty (excluding pins and printheads) in one-year increments. LM60 and LM210 microarrayers utilize 48 printing pins of any tip size from the Professional, 946 or Stealth pin series. Pro3 pins are our most popular pin choice for users placing pin durability over upfront cost as Pro3 pins are rated at 10 million printing cycles. LM60 and LM210 microarrayers utilize 48 printing pins of any tip size from the Professional, 946 or Stealth pin series. 946MP3 pins are a popular choice for many research and commercial laboratories. LM60 and LM210 microarrayers are equipped with a 48-pin printhead designed with an attachment bayonet for easy attachment and removal. Conveyor-based worktable workstation for the LM60 and LM210 microarrayers, allowing fully automated loading and unloading of slide, microplate and custom cassette worktables from the deck. Custom anti-vibration table for the Anti-Vibration LM60 and LM210 microarrayer systems. Table 22 Price (US dollars)* LM60 $125,475 LM210 $204,225 NPW60 $12,548 NPW210 $20,423 Pro3 $ MP3 $461 NPSPH48 $1,838 NPA NPA Inquire Inquire
23 Microplate Stacker 189 Microplate Stacker 42 Microplate stacker holding 189 microplates with single plate loader, humidity and refrigeration controls, barcode reader, and lid lifter. Integrates directly with both the LM60 and LM210 systems. Microplate stacker holding 42 microplates with single plate loader, humidity and refrigeration controls, barcode reader, and lid lifter. Integrates directly with both the LM60 and LM210 systems. Apparatus that permits dehumidification of the Dehumidificati printing chamber. Compatible with the on Apparatus LM60 and LM210 systems. Triple Microplate Cooling Single Microplate Cooling Microplate Worktables Custom Worktables Microarray Instrument Cleaner Liquid cooling apparatus driven by a Peltier device for all three microplate deck positions. Reduces sample evaporation for both the LM60 and LM210 systems. Not compatible with the Microplate Stackers. Peltier cooling apparatus for a single microplate deck position. Reduces sample evaporation for both the LM60 and LM210 systems. Not compatible with the Microplate Stackers. Microplate worktables insert directly into the LM60 or LM210 deck, allowing the user to print directly into well microplates (LM60) or well microplates (LM210). Worktables are easily removed to allow switching between glass substrate slides and microplates. Custom microplate worktables insert directly into the LM60 or LM210 deck, allowing the user to print onto their own proprietary cassettes or cartridges. Worktables are easily removed to allow switching between glass substrate slides, microplates, and custom cassettes and cartridges. Arrayit High-Performance Instrument Cleaner removes dust, particulates, fingerprints and other contaminants from microarrayers. Apply using pump nozzle and Cleanroom Wipes, 65 ml of 1X solution. NPA NPA NPA NPA NPA NPA NPA Inquire Inquire Inquire Inquire Inquire Inquire Inquire AIC $100 microarrayers are configured individually for each customer based on the number and type of printing pins, deck configuration, and options. *Please contact us by telephone , fax , arrayit@arrayit.com, or click on the purchase buttons above to proceed directly to the purchase page. *International pricing may vary by as much as 30% or more depending on country due to import duties, stocking fees and technical support. *Add shipping and handling to all orders. 23
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