NEMESYS Hardware Manual and Reference
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1 NEMESYS Hardware Manual and Reference ORIGINAL INSTRUCTIONS 2.08 AUGUST 2018
2 CETONI GmbH Wiesenring Korbussen Germany T +49 (0) F +49 (0) E info@cetoni.de 2 nemesys Hardware Manual
3 The information and data contained in this document are subject to change without notice. CETONI GmbH is constantly striving to develop all its products. This means that there may be changes in form, equipment and technology. Claims cannot therefore be made on the basis of information, illustration or descriptions in these instructions. The description for the product specification in this manual does not constitute an integral part of the contract. If you control the products with a software from CETONI GmbH, you agree to the applicable license agreement, which can be read in the corresponding software manual. This and all other current product manuals can be found at The reproduction, distribution and utilization of this document as well as the communication of its contents to others without explicit authorization are prohibited. Offenders will be held liable for the payment of damages. We are always open to comments, corrections and requests. Please send them to info@cetoni.de. The general terms and conditions of CETONI GmbH shall apply. Alternative agreements must be in written form. Copyright CETONI GmbH Automation and Microsystems. All rights reserved. nemesys Hardware Manual 3
4 1 Overviews and Directories 1.1 Table of Contents 1 Overviews and Directories Table of Contents Revision History 7 2 Introduction Foreword Symbols and Key Words Used Norms and Guide Lines Application Purpose General Description of the Advice Intended Use Reasonably Foreseeable Faulty Application Safety Advice Measures for Safe Operation Safety Devices on the System Condition of the Devices Warranty and Liability Scope of Supply 13 3 Initial Start-Up Software Installation System Configuration Connecting the Base Module Connecting Additional Modules Module Configuration 18 4 Base Module BASE nemesys Hardware Manual
5 4.1 Technical Data Environment Mechanical Data Electrical Data Interfaces Transport and Storage Maintenance and Care Hardware Operation 21 5 Low Pressure Syringe Pump Technical Data Environment Mechanical Data Electrical Data Interfaces Dosing Performance Valve Transport and Storage Maintenance and Care Hardware Operation Mounting a Syringe Fluidic/Valve 29 6 Mid Pressure Syringe Pump Technical Data Environment Mechanical Data Electrical Data Interfaces Dosing Performance Transport and Storage Maintenance and Care 35 nemesys Hardware Manual 5
6 6.4 Hardware Operation Mounting a Syringe Fluidic/Valve Mounting the Blank Holder 41 7 High Pressure Syringe Pump Technical Data Environment Wetted Parts Mechanical Data Electrical Data Interfaces Dosing Performance Transport and Storage Maintenance and Care Hardware Operation Mounting the Safety Hood Fluidic Connections Mounting a Syringe Pressure Sensor 51 8 Accessory Port 53 9 RS232 Connection Pin Assignment of Module Interfaces OEM RS232 Cable Set RS232 Wiring Communication Settings Pin Assignment of the RS232 Cable Disposal 57 6 nemesys Hardware Manual
7 1.2 Revision History REV DATE CHANGE Manuals merged Adaption of the pin assignment table Correction of pin assignment table Removed cable colors for I/O interface Added safety advices about the Pressure Equipment Directive Correction of the pin assignment of the I/O-Interface for the High Pressure Syringe Pump New Corporate Design Correction of pin assignment table Pictures BASE module and USB cable updated Pressure sensor type in High Pressure Syringe Pump changed nemesys Hardware Manual 7
8 2 Introduction 2.1 Foreword Thank you for deciding to purchase a CETONI product. We would like to support you with this handbook as far as possible in your interaction with the nemesys syringe pump system. We are directly available for any questions or suggestions that you may have. The nemesys syringe pump system may only be taken in operation after carefully reading and understanding this manual. We wish you much success in your work with the nemesys syringe pump system. 2.2 Symbols and Key Words Used The following symbols are used in this manual and are designed to aid your navigation through this document: HINT. Describes practical tips and useful information to facilitate the handling of the software. IMPORTANT. Signifies important hints and other useful information that may not result in potentially dangerous or harmful situations. ATTENTION. Identifies a potentially harmful situation. Failure to avert this situation may result in damage to the product or anything in its proximity. CAUTION. Indicates a potentially dangerous situation. Failure to avert this situation may result in light or minor injuries or property damage. 8 nemesys Hardware Manual
9 2.3 Norms and Guide Lines CETONI GmbH declares under its sole responsibility, that the individual nemesys devices and the entire nemesys syringe pump system comply with the health and safety requirements of the relevant European directives. 2.4 Application Purpose General Description of the Advice The nemesys devices are syringe pumps. They allow emptying and filling syringes by the relative linear movement of a syringe- and a piston holder Intended Use The nemesys syringe pump system serves for precise and pulsation-free dosing of fluids in the range of nanolitres per second up to millilitres per second. Pressures of up to several hundred bar can be reached depending on the device. Application usually takes place in laboratory-like rooms Reasonably Foreseeable Faulty Application A use for applications distinct from the intended purpose can lead to dangerous situations and is to be omitted. CAUTION. The unit must not be used as a medical device or for medical purposes. nemesys Hardware Manual 9
10 2.4.4 Safety Advice The safety of the user and a failure-free operation of the devices are assured only if original parts are used. Only original accessories may be used. Warranty claims will not be accepted for damage due to the use of alien accessories or expendables. The devices have been developed and constructed in such a way as to largely rule out hazards due to its intended use. Nevertheless, you must observe the following security measures in order to exclude any remaining hazards: CETONI GmbH points out the responsibilities of the operator for the operation of the devices. The laws and regulations of the place of installation must be observed while operating the devices! To ensure a safe work routine, operators and users must assume responsibility for adhering to regulations. The devices must not be used as a medical device or for medical purposes. Before operating the unit, the user must at all times ensure the operational reliability and the adequate and orderly condition of the unit. The user must be familiar with the operation of the devices and the software. The devices and pipes must be checked for damage before operation. Damaged pipes and plug devices must be replaced immediately. Cables must be laid in a way that avoids any risk of stumbling. Any moving parts must not be touched whilst the devices are in operation. There is a risk of crushing! It is not allowed to use the devices in an explosive atmosphere or with potentially explosive substances. The device is designed and approved to work in fluidic systems, which fall within the scope of Article 4 Paragraph 3 of the Pressure Equipment Directive 2014/68/EU. This means that the system may not exceed a maximum volume of 1 liter. With the use of fluids from Group 1 according to Article 13 of the Pressure Equipment Directive 2014/68/EU, the maximum allowable system pressure is 200 bar. For fluids from Group 2 it is 1000 bar. If different, product-specific values for the maximum pressure are given in the section "Technical Data", these values must be complied with. Regarding the maximum operating temperature, the specification from the section "Technical Data" must be observed. CETONI GmbH is not liable for consequences that may arise if the user expands the system by peripheral devices, such that one of the values or both values are exceeded. 10 nemesys Hardware Manual
11 It is the user's responsibility to become familiar with the mentioned Pressure Equipment Directive and to comply with the prevailing requirements. Wear protective glasses if you are working with corrosive, hot or otherwise dangerous substances during assembly work on the device. Transportation, storage or operation of the devices below 0 C with water in the fluid passages may cause damage to the modules Measures for Safe Operation ELECTROMAGNETIC EMISSIONS The Qmix system is intended for use in any type of facility, connected directly to the public power supply network that supplies buildings used for domestic purposes ELECTROSTATIC DISCHARGE Floors should be made of wood, concrete, or ceramic tiles. If the flooring is made of a synthetic material; the relative humidity must be at least 30% ELECTRIC DISTURBANCES The quality of the supply voltage should be to the standard of a typical business or hospital environment MAGNETIC DISTURBANCES Do not place power connector cables, even of other appliances, in close proximity of the devices and their cables. Mobile communication devices may not be used in closer proximity of the devices or their cables than the recommended safety distance! Safety Devices on the System The system can be switched off at any time in an emergency using the mains switch on the Base Module (rocker switch on the side of the housing); this will cause no damage to the unit Condition of the Devices Irrespective of the faultless manufacture of the devices, damage can occur whilst the unit is in operation. With this in mind, always carry out a visual check of the components mentioned before use. Pay particular attention to crushed cables, damaged tubing, and deformed plugs. If you should notice any damage, please do not use the devices and inform CETONI GmbH without delay. CETONI will put nemesys Hardware Manual 11
12 your devices back to an operational condition at the earliest. Do not attempt to repair the devices yourself. 2.5 Warranty and Liability The devices left our company in perfect condition. Only the manufacturer is permitted to open the devices. All guarantee and liability entitlements, particularly damage entitlements due to personal injuries, are void if the devices are opened by an unauthorized person. The duration of the warranty is 1 year from the day of delivery. It is not extended or renewed due to work carried out under warranty. CETONI GmbH considers itself responsible for the devices with regard to safety, reliability and function only if assembly, new settings, changes, extensions and repairs are carried out by CETONI GmbH or an authorized centre, and if the devices have been used in accordance with the instruction manual. The nemesys syringe pump system conforms to the basic safety regulation standards. Industrial property rights are reserved on the circuits, methods, names, software programs, and units. 12 nemesys Hardware Manual
13 2.6 Scope of Supply The delivery should correspond to the order. The following items should be included in the scope of supply of the base module: COUNTRY SPECIFIC POWER CABLE FOR NON- HEATING APPARATUS USB CABLE 10 m ACTIVE BUS TERMINATING PLUG HARDWARE-MANUAL AND CD WITH SOFTWARE- MANUAL, SOFTWARE AND DRIVERS nemesys Hardware Manual 13
14 3 Initial Start-Up IMPORTANT. Please carefully read this manual and the associated software manual in their entirety before starting up your nemesys syringe pump system. To ensure failure-free initial start-up of your syringe pump system, the following sequence must be adhered to. The individual steps are described in more detail in the sections below. Software Installation Before initially connecting the devices to a PC System Configuration Adding modules to the system one by one Module Configuration Setting syringe size, pressure limits 3.1 Software Installation Before connecting the system, the supplied software and drivers must be installed. The procedure is described in the associated software manual, which you can find on the supplied CD or USB stick. IMPORTANT. Install the software and device drivers as described in the software manual, before connecting your device to a PC via USB. 3.2 System Configuration Place your nemesys modules on a firm and level surface in the desired sequence, without connecting them. You can arrange the system horizontally or vertically. 14 nemesys Hardware Manual
15 ATTENTION. In vertical arrangement the assembly will be less stable. Please take precautions to avoid tipping and place the devices at least 40 cm from the edge of the table Connecting the Base Module After installing the software and device driver, connect the USB connector of the base module (USB type B) with a free USB connector on your PC (USB type A). Plug the base module into a power outlet using the supplied power cable. The device can be connected to AC power sources ranging from 90 to 264 volts and 47 to 63 Hz. USB-socket (type B) Fuse Power socket Power switch CAUTION. Danger of injury due to damaged power lines and plugs! Check the device and power lines for damage! Never operate the device with damaged power lines or plugs! Only use supplied cables. CAUTION. Danger of stumbling due to connecting cables! Place cables in such way as to avoid any danger of stumbling! nemesys Hardware Manual 15
16 Flip the power switch to turn on the device. The on/off switch should light up while the device is turned on. If this is not the case, make sure that the power cord is firmly connected to the device and the power outlet Connecting Additional Modules The modules must be configured individually by the software before they can be used. The procedure is slightly different depending on whether you use the nemesys UserInterface or QmixElements. The exact process is described in the respective software manuals and will be touched on briefly here: In case of the nemesys UserInterface the modules are added to the base module one by one, before being configured. According to this sequence, each module is assigned a number internally. That is the reason devices have to be reset through Remove Dosing Unit before they can be used in a different system. This also resets the assigned number. In QmixElements every module is plugged into the base module, configured and unplugged individually. Once all modules are configured, the system can be assembled. Following, we will describe the mechanical connection of additional modules. To ensure correct function of the overall system, please read and observe the respective section in the associated software manual, before connecting additional modules. IMPORTANT. Please read and observe the respective section of the associated software manual before connecting additional devices. 16 nemesys Hardware Manual
17 Place the module you would like to connect next to your base module or your existing system in such way that the centering pins of the final system module face the centering holes of the module to be connected. Plug the new module into the system. The centering pins are inserted into the respective centering holes and the plug connectors are connected. To ensure clean contact between the modules, both modules must make physical contact across their entire surface. Avoid canting the modules. Connecting additional modules Insert the bus termination plug into the last connected module of your system. Make sure that this plug is always plugged in before the system is turned on. Otherwise, there may be disruptions in data communication. IMPORTANT. Always insert the bus termination plug into the socket of the final connected system. Otherwise there may be disruptions in data communication. nemesys Hardware Manual 17
18 Connecting Terminator Plug IMPORTANT. Deactivate the standby/sleep mode of your PC to avoid malfunctions. 3.3 Module Configuration Among other things, module configuration includes inserting syringes, using valves and connecting periphery devices. All necessary information with respect to technical data and hardware operation as well as configuration of the various models can be found in the following module-specific sections of this manual. If software actions become necessary, the manual will point you to the software manual at the appropriate time. There you will also find additional and helpful information. 18 nemesys Hardware Manual
19 4 Base Module BASE Technical Data Environment OPERATING TEMPERATURE 0 C to 50 C STORAGE TEMPERATURE -20 C to 75 C OPERATING AIR HUMIDI TY 20% to 90%, non-condensing STORAGE AIR HUMIDITY 20% to 90%, non-condensing Mechanical Data DIMENSIONS (L X W X H) WEIGHT 310 x 100 x 56 mm 1800 g nemesys Hardware Manual 19
20 4.1.3 Electrical Data SUPPLY VOLTAGE FREQUEN CY 90 to 260 VAC 47 to 63 Hz POWER OU TPUT 24 VDC, 5A, 120 W Interfaces USB 1.1 and Transport and Storage Please do not lift or transport the modules while they are plugged into each other. Transport in assembled state is only permissible when using the original packaging. Please refer to section for information about storage. ATTENTION. Danger of damaging the device! Do not transport modules while they are plugged into each other. 4.3 Maintenance and Care When used properly, the device is maintenance-free. In case of problems that you cannot fix yourself or that require opening the device, please contact CETONI GmbH to coordinate any further actions. The device may be opened only by CETONI GmbH or authorized service personnel. Failure to adhere to this rule will void the warranty. The software manual includes detailed information about malfunctions with respect to the operating software. Wipe the device with a moist (not wet) cloth in such way that no liquids get into the inside. In case of heavy soiling you may use some detergent or alcohol. 20 nemesys Hardware Manual
21 4.4 Hardware Operation The base module supplies all other modules with power and serves as an interface to your PC. The BASE 120 base module can continuously supply a power of 120 W. Under the technical data electrical data section you can find the power consumption of every module you may want to connect. Please make sure that the total power consumption of the assembly must not exceed the power supply capability of the base module. In case of overload the base module will deactivate itself and all connected devices and you will lose control of your application. Should the power supply capacity of the BASE 120 base module be insufficient, you may also use the BASE 600 base module or the BASE 600XT extension. ATTENTION. Danger of sudden deactivation! Make sure that the total power consumption of all connected devices does not exceed the power supply capacity of the base module. On one side of the device there is a power connector with a fuse box. A replacement fuse (Ø5x20 mm) can be found in the same box. The rated current of the fuse may vary depending on the country of use and can be seen on the type plate. The power switch is located to the right of the power socket and will light up red when the device is turned on. The type B USB-connector is used to connect the device to your PC. USB connector (type B) Fuse Power socket Power switch nemesys Hardware Manual 21
22 5 Low Pressure Syringe Pump 5.1 Technical Data Environment OPERATING TEMPERATU RE 0 C to 45 C STORAGE TEMPERATURE -20 C to 75 C OPERATION AIR HUMIDITY 20% to 90%, non-condensing STORAGE AIR HUMIDITY 20% to 90%, non-condensing Mechanical Data DIMENSIONS (L X W X H) WEIGHT 310 x 47 x 56 mm 1300 g 22 nemesys Hardware Manual
23 5.1.3 Electrical Data SUPPLY VOLTAGE CURRENT DRAW 24 VDC 0,3 A POWER CONSUMPTION 7 W Interfaces CAN 1 Mbit/s RS-232 section 9 ACCESSORY PORT section Dosing Performance The following table provides an overview of minimum and maximum dosing speeds of the various gear configurations as well as the resulting flow rates, using the example of a 1 ml syringe with a 60 mm stroke. Dosing precision slowly decreases below the speeds and flow rates referred to as pulsation-free. Gear w/o STANDARD Special Min. speed [µm/s] 0,065 0,065 0,065 Min. pulsation free speed [µm/s] 14,648 1,042 0,502 Max. speed [mm/s] 89 6,33 3,06 Min. flow [µl/min] 0,065 0,065 0,065 1 ml syringe with 60mm stroke Min. pulsation-free flow [µl/min] 14,648 1,042 0,502 Max. flow [ml/min] 89 6,33 3,05 nemesys Hardware Manual 23
24 5.1.6 Valve TECHNICAL DATA HOUSING MATERIAL SEALING MATERIAL PEEK FFKM (perfluoroelastomer) MEDIA TEMPERATURE 0 to +50 C MAX. VISCOSITY 21 mm²/s INTERNAL VOLUME approx. 45 µl MAX. PRESSURE 3 bar NOMINAL SIZE 0,6 mm FLUIDIC CONNECTIONS ¼ 28 UNF 5.2 Transport and Storage Please do not lift or transport the modules while they are plugged into each other. Transport in assembled state is only permissible when using the original packaging. Use the original packaging for shipping the modules. Please observe the information provided in with respect to storage. 24 nemesys Hardware Manual
25 ATTENTION. Danger of damaging the device! Never transport modules while they are plugged into each other. 5.3 Maintenance and Care When used properly, the device is maintenance-free. In case of problems that you cannot fix yourself or that require opening the device, please contact CETONI GmbH to coordinate any further actions. The device may be opened only by CETONI GmbH or authorized service personnel. Failure to adhere to this rule will void the warranty. The software manual includes detailed information about malfunctions with respect to the operating software. Wipe the device with a moist (not wet) cloth in such way that no liquids get into the inside. In case of heavy soiling you may use some detergent or alcohol. 5.4 Hardware Operation Connect the Low Pressure Syringe Pump to your base module / system as described in section and the software manual. If the module has not been configured, you will be required to perform a reference move during the configuration process. During the reference move the piston holder will move to its front position and be synchronized with the software display. To avoid damage the reference move may only be performed without a syringe. After deactivating the base module the piston holder can be moved by applying some force (e.g. through residual pressure in the system). Therefore, it is sensible to repeat the reference run from time to time. ATTENTION. The reference move must be performed without syringe. Otherwise the device or the syringe may be damaged. CAUTION. Do not touch any moving parts on the device during operation! There is a danger of crushing. nemesys Hardware Manual 25
26 5.4.1 Mounting a Syringe The syringe holder of Low Pressure Syringe Pump allows the use of syringes with outside diameters ranging from 6 to 30 mm and a piston stroke of up to 65 mm. IMPORTANT. Please use high-quality glass syringes with outside diameters ranging from 6 to 30 mm to ensure precise flow rates. Before mounting a syringe to the Low Pressure Syringe Pump, it must be configured and selected in the operating software. The respective process is described in the software manual. You need the volume (scale volume), the nominal stroke (scale length) and the total stroke (piston stroke), which may be different. Scale Length Scale Volume Piston Stroke 26 nemesys Hardware Manual
27 Use the following process to mount a syringe to the Low Pressure Syringe Pump: Loosen the knurled screw of the syringe receptacle. Now you can push the bracket off the pins and lift it up. Disassembly syringe holder Loosen the knurled screw of the piston holder and remove the adapter plate. Lift up the clamping piece. Disassembly piston holder In order to make use of the entire syringe volume, move the piston holder to the front position through the software. Place an empty syringe on the remaining base of the syringe holder in such way that the piston touches the piston holder. The syringe position can be varied somewhat by moving the piston holder. For this purpose loosen the screw with a 4 mm Allen wrench. nemesys Hardware Manual 27
28 ATTENTION. To avoid damage, please make sure that the remaining syringe stroke is always the same as or larger than that of the module. Screw Replace the bracket of the syringe holder, insert into the pins and lock the syringe by using the large knurled screw. Replace the clamping piece and insert the adapter plate suitable for the piston diameter in such way that the piston plate is located between piston holder and adapter plate. The piston is locked in place by slightly tightening the knurled screw on the back. Make sure that syringe and syringe piston are in alignment. 28 nemesys Hardware Manual
29 IMPORTANT. The syringes, particularly the seals, are wear parts. Check them on a regular basis and replace them, if necessary Fluidic/Valve The Low Pressure Syringe Pump can be fitted with an optional valve. The valve allows you to switch the syringe connection between your application (outlet) and a reservoir (intake), allowing an automatic refilling of the syringe. Through the software, you can set the valve in such way that it automatically switches to the intake during filling. The three connections are fitted with a ¼ -28 UNF thread and therefore allow the use of common HPLC fittings. Outlet (normally open) Intake (normally closed) Syringe connection A rocker inside the valve (green) connects the syringe port (1) alternatively with the outlet (2) or the intake (3). In the following image the syringe is connected with the outlet (blue), while the intake is closed (red). A FFKM membrane connected to the rocker (shaded in green) seals the fluidic system. This membrane limits the operating pressure of the valve to 3 bar. nemesys Hardware Manual 29
30 ATTENTION. To avoid damaging the valve, please observe the maximum operating pressure of 3 bar. ATTENTION. Before using the valve, please check the chemical resistance of the PEEK housing material and the FFKM sealing material (perfluoroelastomer) against the dosing liquid VALVE INSTALLATION AND REMOVAL The valve can be removed from the device in a few simple steps. This simplifies the fitting of tubes as well as cleaning. It also allows operation without a valve, in cases where it is not needed. To remove the valve press the rocking catch at the plug and remove the plug. When replacing it later please observe the orientation of the plug (the white surface must face the valve, the rocking catch must face away from the valve). 30 nemesys Hardware Manual
31 ATTENTION. Observe the orientation of the valve plug (the white surface must face the valve, the rocking catch must face away from the valve). Rocking Catch In the next step you can simply pull up the valve to remove it. nemesys Hardware Manual 31
32 6 Mid Pressure Syringe Pump 6.1 Technical Data Environment OPERATING TEMPERATUR E 0 C to 45 C STORAGE TEMPERATURE -20 C to 75 C OPERATING AIT HUMIDITY 20% to 90%, non-condensing STORAGE AIR HUMIDITY 20% to 90%, non-condensing Mechanical Data DIMENSIONS (L X W X H) WEIGHT 310 x 59 x 60 mm 2000 g 32 nemesys Hardware Manual
33 6.1.3 Electrical Data SUPPLY VOLTAGE CURRENT DRAIN 24 VDC 0,3 A POWER CONSUMPTION 15 W Interfaces CAN 1 Mbit/s RS-232 section 9 ACCESSORY PORT section 8 nemesys Hardware Manual 33
34 6.1.5 Dosing Performance The following table provides an overview of minimum and maximum dosing speeds. The resulting flow rates shown here are based on the example of a 1 ml glass syringe with a 60 mm stroke as well as CETONI stainless steel syringes. Dosing precision slowly decreases below the speeds and flow rates referred to as pulsation-free. The table also indicates the maximum pressure. In case of a glass syringe, the syringe itself is the limiting element. In case of stainless steel syringes it is the force of the module. You need the nominal and maximum stroke for the software configuration of syringes. Please read the relevant sections in the software and syringe manual. SPEEDS MIN [µm/s] MIN PULSATION-FREE [µm/s] MAX [MM/S] 0,065 1,005 6,00 SYRIN GE NOMINAL STROKE/ MAX. STROKE [MM]. MAX. PRESSURE MIN [µl/min] FLOW RATES MIN PULSATION-FREE [µl/min] MAX [ML/S] 1 ML-ILS GLASS SYRIN GE bar 530 psi 0,065 1,005 0,10 2,5 ML 49, ML 52, ML 49, ML 50, ML 51, bar 2900 psi 100 bar 1500 psi 50 bar 720 psi 20 bar 290 psi 10 bar 140 psi 0,196 3,015 0,30 0,370 5,703 0,57 0,784 12,083 1,20 1,923 29,631 2,95 3,768 58,064 5,78 34 nemesys Hardware Manual
35 6.2 Transport and Storage Please do not lift or transport the modules while they are plugged into each other. Transport in assembled state is only permissible when using the original packaging. Use the original packaging for shipping the modules. Please observe the information provided in with respect to storage. ATTENTION. Danger of damaging the device. Do not transport modules while they are plugged into each other! 6.3 Maintenance and Care When used properly, the device is maintenance-free. In case of problems that you cannot fix yourself or that require opening the device, please contact CETONI GmbH to coordinate any further actions. The device may be opened only by CETONI GmbH or authorized service personnel. Failure to adhere to this rule will void the warranty. The software manual includes detailed information about malfunctions with respect to the operating software. Wipe the device with a moist (not wet) cloth in such way that no liquids get into the inside. In case of heavy soiling you may use some detergent or alcohol. 6.4 Hardware Operation Connect the Mid Pressure Syringe Pump to your base module / system as described in section and the software manual. If the module has not been configured, you will be required to perform a reference move during the configuration process. During the reference move the piston holder will move to its front position and be synchronized with the software display. To avoid damage the reference move may only be performed without a syringe. After deactivating the base module the piston holder can be moved by applying some force (e.g. through residual pressure in the system). Therefore, it is sensible to repeat the reference run from time to time. nemesys Hardware Manual 35
36 ATTENTION. The reference move must be performed without a syringe. Otherwise the device or the syringe may be damaged. CAUTION. Do not touch moving parts during operation! There is a danger of crushing Mounting a Syringe The syringe holder of Mid Pressure Syringe Pump allows the use of syringes with a ¼ -28 UNF outside thread at the tip and an M3 thread in the piston. The maximum diameter is 50 mm, the maximum syringe stroke 65 mm. ATTENTION. On the Mid Pressure Syringe Pump please only use syringes offered by CETONI GmbH for this device. When using glass syringes, please make sure not to exceed their maximum operating pressure. Before mounting a syringe to the Mid Pressure Syringe Pump, it must be configured and selected in the operating software. The respective process is described in the software manual. You will need the volume (scale volume), the nominal stroke (scale length) and the maximum stroke (piston stroke), which may be different. The scale volume and scale length values are engraved on CETONI stainless steel syringes. Unless otherwise stated, the piston stroke is 60 mm. These values can also be found in the table in section For more information with respect to syringes, please refer to the syringe manual. 36 nemesys Hardware Manual
37 Scale Volume Scale Length Please follow these steps to mount a syringe on the Mid Pressure Syringe Pump: Loosen the screws on the syringe holder and push it all the way to the front, or completely remove it from the device. Loosen the knurled screws of the holding-down clamp, if installed. Knurled screws Fasting screw nemesys Hardware Manual 37
38 Screw the syringe into the syringe holder. Use one of the supplied PTFE disks to achieve a sealed connection. PTFE disk In order to make use of the entire syringe volume, move the piston holder to the front position through the software. Push back the syringe holder until the piston touches the piston holder. Retighten the fastening screws of the syringe holder. Lock the syringe piston with the knurled screw. To avoid bending the syringe in case of high pressure, lower the blank holder onto the syringe and fasten the two knurled screws. 38 nemesys Hardware Manual
39 Blank holder Knurled screw IMPORTANT. Syringes, and particularly the seals, are wear parts. Check them on a regular basis and replace them if necessary. ATTENTION. To avoid damage, please make sure that the remaining syringe stroke is always larger than or the same as that of the module. ATTENTION. Before use, please check the resistance of syringe seals against the dosing medium. Use a different material, if necessary Fluidic/Valve The syringe holder of the Mid Pressure Syringe Pump offers two ¼ -28 UNF threaded connections on the output side, allowing the use of common HPLC connectors. The Mid Pressure Syringe Pump can be fitted with an optional pair of non-return valves. They allow automatic switching of the syringe connector between your application (outlet) and a reservoir (intake) and thereby automatic refilling of the syringe. Please refer to the associated valve manual for more detailed information about valves. The valves are simply plugged onto the corresponding pins on the module. nemesys Hardware Manual 39
40 Connect tubes between the syringe holder and the valves as follows: Intake Outlet ATTENTION. Before using the valve please check its chemical resistance against the media you intend to pump. 40 nemesys Hardware Manual
41 6.4.3 Mounting the Blank Holder The blank holder prevents the syringe from bending upward, which is an effect that occurs in case of high pressure. As a consequence syringe wear is decreased as well. To mount the blank holder simply place it into the two gaps on the device and fasten it on both sides with the provided screws (2 mm Allen head). nemesys Hardware Manual 41
42 7 High Pressure Syringe Pump 7.1 Technical Data Environment OPERATING TEMPERATURE 0 C to 45 C STORAGE TEMPERATURE -20 C to 75 C OPERATING AIR HUMIDI TY 20% to 90%, non-condensing STORAGE AIR HUMIDITY 20% to 90%, non-condensing Wetted Parts SYRIN GE HOLDER AND FITTING PRESSU RE SENSOR Stainless steel (316L) Aluminium oxide (Al2O3) PRESSU RE SENSOR SEAL Perfluoroelastomer FFKM 42 nemesys Hardware Manual
43 7.1.3 Mechanical Data DIMENSIONS (L X W X H) WEIGHT 310 x 110 x 136 mm 4500 g Electrical Data SUPPLY VOLTAGE CURRENT DRAIN 24 VDC 1,9 A POWER CONSUMPTION 45 W Interfaces CAN 1 Mbit/s RS-232 section 9 ACCESSORY PORT section 8 nemesys Hardware Manual 43
44 7.1.6 Dosing Performance The following table provides an overview of minimum and maximum dosing speeds. The resulting flow rates shown here are based on CETONI stainless steel syringes. Dosing precision slowly decreases below the speeds and flow rates referred to as pulsation-free. The table also indicates the maximum pressure that can be achieved with the High Pressure Syringe Pump in combination with the respective syringe. You need the nominal stroke and the maximum stroke for the software configuration of syringes. Please read the relevant sections in the software and syringe manual. SPEED MIN [µm/s] MIN PULSATION-FREE [µm/s] MAX [MM/S] 0,061 0,636 7,00 SYRIN GE NOMINAL / MAX STROKE [MM]. MAX. PRESSURE MIN [µl/min] FLOW RATES MIN PULSATION-FREE [µl/min] MAX [ML/S] 2,5 ML 49, ML 52, ML 49, ML 50, ML 51, ML 50, bar 7400 psi 270 bar 3900 psi 120 bar 1740 psi 50 bar 360 psi 25 bar 360 psi 12 bar 175 psi 0,182 1,908 0,35 0,344 3,609 0,66 0,728 7,646 1,40 1,785 18,751 3,44 3,499 36,743 6,74 7,137 74,960 13,76 44 nemesys Hardware Manual
45 7.2 Transport and Storage Please do not lift or transport the modules while they are plugged into each other. Transport in assembled state is only permissible when using the original packaging. Use the original packaging for shipping the modules. Please observe the information provided in with respect to storage. ATTENTION. Danger of damaging the device. Do not transport modules while they are plugged into each other. 7.3 Maintenance and Care When used properly, the device is maintenance-free. In case of problems that you cannot fix yourself or that require opening the device, please contact CETONI GmbH to coordinate any further actions. The device may be opened only by CETONI GmbH or authorized service personnel. Failure to adhere to this rule will void the warranty. The software manual includes detailed information about malfunctions with respect to the operating software. Wipe the device with a moist (not wet) cloth in such way that no liquids get into the inside. In case of heavy soiling you may use some detergent or alcohol. 7.4 Hardware Operation Connect the High Pressure Syringe Pump to your base module / system as described in section and the software manual. The High Pressure Syringe Pump only works with an installed safety hood and may only be operated with it in place. Please refer to section for more information about using the safety hood. If the module has not been configured, you will be required to perform a reference move during the configuration process. During the reference move the piston holder will move to its front position and be synchronized with the software display. To avoid damage the reference move may only be performed without a syringe. nemesys Hardware Manual 45
46 After deactivating the base module the piston holder can be moved by applying some force (e.g. through residual pressure in the system). Therefore, it is sensible to repeat the reference run from time to time. ATTENTION. The reference move must be performed without a syringe. Otherwise the device or the syringe may be damaged Mounting the Safety Hood Place the safety hood on the device in such way that the pins slide into the gaps provided for them (blue in image). Push down on the opposite end of the hood until the spring-loaded balls (also shown in blue) settle into the holes provided for them in the hood. To remove the hood, simply reverse this process. Pins Balls CAUTION. Only use the High Pressure Syringe Pump with the mounted safety hood! Do not touch any moving parts on the device during operation! IMPORTANT. If you remove the safety hood during operation, the High Pressure Syringe Pump will stop automatically. 46 nemesys Hardware Manual
47 7.4.2 Fluidic Connections A Swagelok tube fitting is used for fluidic connection. It is suitable for using capillaries made from metal (e.g. stainless steel, titanium) and plastic (e.g. PTFE, PEEK). Please refer to information provided by the respective manufacturer with respect to maximum pressure. The High Pressure Syringe Pump is supplied with two tube fittings for capillaries with an external diameter of 1/16 and 1/18. Following is a description of using the tube fittings: FIRST-TIME INSTALLATION (1) Fully insert the tube / hose into the fitting and against the shoulder; rotate the nut finger-tight. (2) Mark the nut at the 6 o clock position. (3) Tighten the nut three-quarters turn to the 3 o clock position with an open-end wrench DISMANTLING ATTENTION. Release pressure from the system before loosening the fittings. (1) Before dismantling, draw a marker line across the nut and the fitting body. In this way you create a reference for retightening the cap nut to exactly the same position it was in before. (2) Pull out the capillaries. The nut and the ferrules remain on the capillary REASSEMBLY (1) To reassemble, insert the capillary with preassembled ferrules into the fitting body until the front ferrule seats against the fitting body. (2) Rotate the nut with open-end wrench to the previously pulled-up position as indicated by the marks you made before; at this point you will feel a significant increase in resistance. (3) Retighten the nut slightly. Done! You can purchase additional connecting material from Swagelok, such as replacement clamping rings (ordering number SS-100-SET). nemesys Hardware Manual 47
48 IMPORTANT. Only use capillaries specified for the anticipated pressure levels. The supplied 1/16 PEEK tubes are suitable for pressures up to 200 bar. ATTENTION. After connecting, check the tightness of all fluidic connections on a regular basis REPLACING THE CONDUIT FITTING The tube fitting is screwed into the syringe holder and can be loosened or tightened with a 9/16 open end wrench. For this purpose, place the wrench onto the large hexagon nut (not the small one of the cap nut)! There is a metal bushing behind the conduit fitting for sealing purposes. This bushing can remain in the borehole while the conduit fitting is replaced. Firmly tighten the newly mounted conduit fitting to achieve a sealed connection. 48 nemesys Hardware Manual
49 7.4.3 Mounting a Syringe Before mounting a syringe to the High Pressure Syringe Pump, it must be configured and selected in the operating software. The respective process is described in the software manual. You will need the volume (scale volume), the nominal stroke (scale length) and the maximum stroke (piston stroke), which may be different. The scale volume and scale length values are engraved on CETONI stainless steel syringes. Unless otherwise stated, the piston stroke is 60 mm. These values can also be found in the table in section Additional information can be found in the syringe manual. Scale Volume Scale Length Follow these steps to mount a syringe on the High Pressure Syringe Pump: To be able to screw the syringe into the mounting bore while establishing a sealed connection, please insert one of the provided O-rings (3 mm inside diameter and 1.5 mm cord thickness) into the indentation at the syringe outlet (blue in image). nemesys Hardware Manual 49
50 O-ring Position the piston holder in such way as to make sufficient space to screw in the syringe. Remove the safety hood as described in section Screw the syringe into the syringe holder as far as it will go. Put the safety hood back on the device and advance the piston holder until it touches the piston. Take off the safety hood and attach the piston to the piston holder using the knurled screw. 50 nemesys Hardware Manual
51 After putting the safety hood back on, you are ready for dosing. ATTENTION. Before operation, check the resistance of the syringe seal against the dosing medium. If necessary, replace the seal with a seal made from a different material. IMPORTANT. Syringes, and particularly seals, are wear parts. Check them on a regular basis and replace them if necessary Pressure Sensor There is an integrated pressure sensor in the syringe holder of the High Pressure Syringe Pump. This allows the device to stop automatically upon reaching the preset maximum pressure. ATTENTION. Before using the device, check the chemical resistance of wetted materials against the fluid to be metered. nemesys Hardware Manual 51
52 The pressure sensor must be configured in the software before use. The associated process is described in the software manual. For the configuration you will need the pressure range of the sensor (e.g bar) and the range of the output signal (e.g V). These values can be found on the nameplate on the side of the device under sensor: ATTENTION. Configure the pressure sensor before use, to avoid damaging the device or your application. 52 nemesys Hardware Manual
53 8 Accessory Port The Low, Mid and High Pressure Syringe Pumps, are equipped with an accessory port or can be equipped with it as an optional extra. The additional port allows the use of a pressure sensor, for example. The pin assignment of the connector at the module and the wire colors of the connecting cable, which can be purchased from CETONI, can be found in the table on the next page. Of course, you can also purchase ready-made periphery devices from CETONI GmbH. A matching connector plug is also available from Hirose (order number HR10A-10P-12P(73)). The configuration of pressure sensors is described in the software manual. Read and observe the relevant section before connecting a pressure sensor. IMPORTANT. Pin 1 is not assigned at the accessory port of the High Pressure Syringe Pump, because analogue input AI1 is already used for the built-in pressure sensor. nemesys Hardware Manual 53
54 PIN SIGNAL DESCRIPTION 1 Analog input AI1 0-5 V (to Pin 12) 2 Analog input AI2 0-5 V (to Pin 12) 3 Digital input 1 <0,8 V Low >2 V High 24 V max. 4 Digital input 2 <0,8 V Low >2 V High 24 V max. 5 Digital input 3 <1,7 V Low >4,2 V High 24 V max. 6 Digital output 1 Valve voltage NPN Max. 1 A Active: 0 V (GND) Inactive: open 7 Digital output 2 Switch valve NPN Max. 1 A Active: 0 V (GND) Inactive: open 8 Digital output 3 NPN Max. 1 A Active: 0 V (GND) Inactive: open 9 Digital ground V Out +24 VDC / <1 A V Out +5 VDC / <150 ma 12 Analog ground 54 nemesys Hardware Manual
55 9 RS232 Connection 9.1 Pin Assignment of Module Interfaces PLUG SOCKET A1 1 2 A2 A2 2 1 A PIN 1 Not connected RS232 RX 2 Not connected RS232 TX 3 CAN High CAN High 4 CAN Low CAN Low 5 Signal GND Signal GND A1 +24 V +24 V A2 GND GND 9.2 OEM RS232 Cable Set RS232 Wiring Insert the mixed D-Sub plug of the cable into the socket of the final module. The system should be deactivated when you do this. Tighten both screws on the plug manually. You do not need a bus termination plug, since the plug of the RS232 cable already contains a bus termination resistor. Now, plug the 9-pin D-Sub socket of the cable into an RS232 connection on your PC or other controller. For greater distances to the socket please use a 1:1 cable with a 9-pin D-Sub plug. nemesys Hardware Manual 55
56 Now, you can reactivate your system and send or receive data through RS232. Since every module contains a gateway from RS232 to the system s internal CAN bus, you can now address each module of your system with only one RS232 cable Communication Settings For a functioning communication with the nemesys modules you have to make the following communication settings for the serial interface on your PC or other controller: Baud rate: Data bit rate: 8 Parity: none Stop bits: 1 Flow control: none Pin Assignment of the RS232 Cable The OEM RS232 cable adapts the nemesys device interface to a standard 9-pin D-Sub plug. The following table shows the pin assignment of the nemesys interface and the 9-pin D-Sub: nemesys Interface (socket) 9-pin D-Sub socket A2 2 1 A Pin Cable Pin 1 RS232 RX Orange 3 TXD Transmit Data 2 RS232 TX Brown 2 RXD Receive Data 5 Signal GND Black 5 GND Signal GND D-Sub housing = shielding Shielding 56 nemesys Hardware Manual
57 10 Disposal Please send your old devices back to CETONI GmbH. We will take care of proper disposal according to electric devices regulations. If necessary, please decontaminate the device before sending it back and attach a completed decontamination declaration with your shipment. nemesys Hardware Manual 57
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