Department of Chemistry

Transcription

1 Department of Chemistry Guidelines for the Confocal Laser Scanning Microscopes The Department of Chemistry (CHEM) has two confocal laser scanning microscopes (confocal microscope) for imaging uses: 1. Leica SP5 Confocal Laser Scanning Microscope 2. Leica SPE Confocal Laser Scanning Microscope The following guidelines summarize the procedures and regulations for using the confocal microscopes. The confocal microscope Technical Officer-In-Charge (TO-I-C), Mr. Chiang W L Michael, is responsible for equipment maintenance, user training, and booking moderation of the confocal microscopes. The confocal microscope Staff-In-Charge, Dr. Lo P K Peggy and Dr. Sun H Y, will respond if complex matter arises. This set of guidelines is subject to periodic review. Users CHEM User: Non-CHEM User: CHEM Faculty, research students and staff Non-CHEM Faculty, research students and staff under the CSE Equipment & Facility Sharing System (Fill-in and submit CSE Equipment Use Form in Appendix 1 to Ms. Ng K Y Helen) Training Training is required regardless of any previous experience gained in confocal microscopy elsewhere; New user has to complete CHEM safety training and submit: (1) Risk Assessment Form, and (2) CSE Equipment Sharing Form (for Non-CHEM User) before contacting TO-I-C for equipment training; Submit Registration Form for CHEM Smartlock and On-line Equipment Booking System in Appendix 2 to Mr. Lam K F to register Online Equipment Booking and/or Smartlock System. Authorized User Privileges Users Access Online Equipment Booking System Access Smartlock System of Rooms B1620 & B1623 Access Microscope Computer CHEM User Non-CHEM User via TO-I-C, Faculty & Academic Staff Last Updated: 26 June 2017 Page 1

2 Booking Booking before use is MANDATORY; Advanced booking: 24 hours 7 days via online Equipment Booking System ( Booking Hours: o Peak Hours: Mon Sat (excluding public holidays); o Non-Peak Hours: Mon Sat; Sun & public holidays Maximum Booking Hours for each confocal imaging system (SP5 or SPE): Users Daily Maximum Booking Hours per User Weekly Usage Quota per Research Group Peak Hours Non-Peak Hours Peak Hours Non-Peak Hours CHEM User Non-CHEM User 3 hrs 2 hrs (Restricted to ) No limit * 12 hrs 8 hrs No limit * Remark: * Except for long-term time-lapse experiment of more than 4 hrs, user should get prior approval from supervisor and Confocal Microscope Staff-in-Charge by submitting the Application for Long-term Time-lapse experiment using Confocal Microscope (Appendix 3) 3 days in advance. Long-term time-lapse microscopy experiment: o Should be restricted to non-peak hours; o For booking more than 4 hrs during non-peak hours, user should get prior approval from supervisor and Confocal Microscope Staff-in-Charge by submitting the Application for Longterm Time-lapse experiment using Confocal Microscope (Appendix 3) 3 days in advance and make booking via TO-I-C. User can only make one appointment per Application Form. Vacant time slots of peak hours on the current day (available for ALL users): o Walk-in and first-come first-served; o Make booking (mandatory) via TO-I-C; o Usage will NOT be counted into weekly booking quota even booking hours reached to maximum. TO-I-C has the priority to pre-book time slots for service and maintenance. Cancellation Cancel at first instance if you no longer need the booked time slot; In addition to full charges will apply for no-show/ failure to cancel booking, penalty also imposes for not cancel unused time slots repeatedly: o 1 st time: User receives warning. o 2 nd time: User receives warning and user s supervisor is informed; All reserved but unused time slots will be counted into the weekly quota if applicable. o 3 rd time or above: User receives warning and user s supervisor is informed; All reserved but unused time slots will be counted into the weekly quota if applicable; Forfeit the user s remaining reservations on the current week; Suspend booking for 3 days. Last Updated: 26 June 2017 Page 2

3 Early finish: cancel the rest of your session via TO-I-C (count the unused time slots into weekly quota if not cancel). Late Coming / No Show Leave a note on the microscope stating the time you will start using the microscope if you are late due to unforeseeable reason; Consider as No Show if microscope is not being used and no note is found after 15 minutes of the beginning of the time reserved; Other users can contact TO-I-C and use the microscope in that time slot; Penalty for repeatedly No Show is the same as that for without cancellation. Users Responsibilities Under no circumstances may a user book for time using another person s name, except PI/supervisor; Sign the Log Book before and after use; NEVER, under any circumstances, write irrelevant information on and remove paper from the Log Book; Clean any oil/water objectives you used and remove your own material. Leave the microscope and room clean after each session for next user; Recommend to check the online booking calendar at the end of your session; contact the next user if possible, leaving the system on if he/she is coming, otherwise turn it off. It is not permitted to bring any unauthorized visitors to the Confocal Room; Do NOT lend your CityU ID card to any unauthorized user to enter the Confocal Room; Users are not allowed to install any software to computers of the microscope; Report equipment failure or mishaps immediately to TO-I-C; Users should back up their own data immediately. Data Storage The Laboratory does not make backups of user data. The local hard-disk of the microscope system will be regularly cleaned up in the first Friday of every even month (postpone to the next working day if it is a public holiday) and only files saved on the previous week of the scheduled cleanup day will be retained; ONLY use CD-R/RW or DVD-R/RW to copy data from local computer of the microscope system; If USB devices (e.g. USB flash drive, portable hard disk) are used to back up data, you have to transfer data to the network-attached storage server for copying and refer to procedure posted in the Confocal Microscope Room for details; Files left on the Desktop or without clearly defined owners will be wiped without notice. Laboratory Safety and Emergency Response Familiarize yourself with the closest fire escape route, laboratory safety and emergency instructions posted in the Confocal Room; Last Updated: 26 June 2017 Page 3

4 Users who need to work after 23:00 or overnight in the Confocal Room have to apply and inform FMO in advance, please reference Standing Circular Cir015 - Late Stay in Offices for details; Users should carefully read the Risk Assessment Report attached in the Equipment Log Book of particular confocal imaging system and fully understand the potential hazards of using the Laser equipment; Inform TO I-C if you encounter any enquiry, problem or emergency. Should you have any queries, please contact the Staff-in-charge or Technical Offer-in-charge: Staff-In-Charge: Dr. Peggy LO Dr. H Y SUN Office: B6523 Office: B6716 Tel: Tel: peggylo@cityu.edu.hk hongysun@cityu.edu.hk Technical Officer in Charge: Mr. Michael CHIANG Office: G1617 Tel: bhchiang@cityu.edu.hk Last Updated: 26 June 2017 Page 4

5 Appendix 1 DEPARTMENT OF CHEMISTRY Guidelines of Equipment Sharing by Colleagues within the CSE The following guidelines summarized the procedures for using the equipment in the Department of Chemistry (CHEM) by colleagues within the College of Science and Engineering (CSE). User completes the Equipment Use by Colleagues within the CSE form and submits to CHEM contact person Ms Helen Ng. CHEM technical officers (STO/ TO) will discuss with user to understand his/ her background and see whether the sample can be analysis by CHEM equipment. User has to provide a CSE web-based safety training report to Helen and attend CHEM safety briefing. CHEM technical officer will provide equipment training to the user. *Charges begin when training starts. CHEM will register the faculty into CHEM equipment booking system. Faculty can book equipment as CHEM equipment users. Booking request will be sent to user s supervisor for approval with charges information and copy to Helen and technical officer equipment-in-charge. User starts using CHEM equipment. Last Updated: 26 June 2017 Page 5

6 DEPARTMENT OF CHEMISTRY Equipment Use by Colleagues within the CSE Information of User Department Department: Name of Supervisor: Name of User: Position: Telephone: Position: Telephone Information of Using CHEM Equipment Name of Equipment: Period/ Times/ Date of using: Estimated Charges of Equipment: Project No. and Chop: Remarks: 1. User should follow all the safety regulations of CHEM; 2. User will be responsible for repairs or replacement for any damaged equipment and facilities; and 3. Violation of the above rules will result in restricting user access to CHEM laboratories. Signature of User Date Signature of User s Supervisor Date Departmental Safety Training Date completed: Time & Venue: Verified by: (CTO/ STO) Date: Equipment Training Date completed: Time & Venue: Verified by: (TO) Date: Last Updated: 26 June 2017 Page 6

7 Appendix 2 Last Updated: 26 June 2017 Page 7

8 Last Updated: 26 June 2017 Page 8

9 Appendix 3 Application for Long-term Time-lapse Experiment using Confocal Microscope Name of Applicant: in block letter Department: for Non-CHEM applicant User Type: CHEM User Non-CHEM User (CSE Equipment and Facilities Sharing System) CityU ID Card No.: ( ) Address: Applicant Signed: Lab Contact No.: Date: Experiment Period: Start am / pm Date Time End Date Time am / pm Microscope: Leica SP5 Confocal Laser Scanning Microscope Leica SPE Confocal Laser Scanning Microscope (Please tick as appropriate) Laser to be used: (Please tick as appropriate) Argon Argon Argon Argon HeNe 543 HeNe 633 Multiphoton Supervisor Approval: Prof./Dr. ( ) Name (in block letter) Signature Date Confocal Microscope Staff-in-Charge Approval: Prof./Dr. ( ) Name (in block letter) Signature Date Last Updated: 26 June 2017 Page 9