Chris Main. Extension Cotton Specialist The University of Tennessee
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1 Chris Main Extension Cotton Specialist The University of Tennessee
2 Ignite Tolerance Liberty Link varieties Two gene resistance to glufosinate from bar and pat genes which were first isolated from two species of Streptomyces bacteria.
3 Ignite Tolerance Varieties designated as Single gene tolerance to glufosinate from pat gene pat gene is used as a selectable marker for the cry1f and cry1ac genes
4 PAT Gene? Phosphinothricin-N-acetyltransferase (PAT) is an enzyme isolated from the common soil bacteria Streptomyces v. pat imparts transgenic plants with tolerance to the herbicide glufosinate-ammonium and facilitates the selection of plants containing cry1f and cry1ac. Glufosinate-ammonium was developed from the same bacteria and causes ammonia to build up plant tissues. Excess ammonia disrupts cell membranes and stops photosynthesis eventually killing plants. pat is the antidote to glufosinate-ammonium, allowing the plant to detoxify the herbicide
5 Ignite Tolerance Varieties designated as EPA has ruled that it is a legal application.
6 Disclaimer Any liability associated with Ignite applications to WideStrike cotton varieties is solely that of the user. PhytoGen and Dow AgroSciences do not recommend or warrant the use of glufosinate ammonium herbicides on WideStrike cotton. Bayer CropScience does not recommend or warrant the use of glufosinate ammonium herbicides on WideStrike cotton. All risk of crop damage and loss associated with the use of glufosinate ammonium herbicides on WideStrike cotton remains solely with the user.
7 Literature Weed Management with Glyphosate- and Glufosinatebased Systems in PHY 485 Cotton. J. R. Whitaker, A. C. York, D. L. Jordan, A. S. Culpepper. (Accepted for publication on 10/1/2010) Glufosinate caused minor and transient injury to the crop, but no differences in cotton yield or fiber quality were noted.
8 Tom Barber, Guy Collins, Darrin Dodds, Chris Main
9 Tolerance High Rates Varieties planted: FM 1773 LLB2 PHY 375 Ignite Applications Untreated Checks Single Application o 1-3 leaf cotton (A) Two Applications o o 1-3 leaf cotton (A) 6-8 leaf cotton (B) Phyotoxicity 7, 14 DAA 7, 14, 28 DAB Plant Height, Nodes 14, 28 DAB, EOS Maturity NAWF (28 DAB) NACB (120 DAP) Gin turnout, Lint yield
10 Variety x Rate Parameter Time Parameter Time Parameter Time Injury 7 DAA Nodes 14 DAA Nodes EOS Injury 14 DAA Height 7 DAB NACB EOS Injury 7 DAB Nodes 7 DAB 1 st Pos.Ret. EOS Injury 14 DAB Height 14 DAB Ginout Harvest Injury 28 DAB Nodes 14 DAB Lint Harvest Height Initial Height 28 DAB Mic Harvest Nodes Initial Nodes 28 DAB Length Harvest Height 7 DAA NAWF 28 DAB Strength Harvest Nodes 7 DAA Ht:Node EOS Uniformity Harvest Height 14 DAA Height EOS
11 Cotton Injury 7 Days After A Treatment LSD = PHY 375 FM 1773 LLB2 Check 1 app 2 app 29 oz 58 oz 87 oz 116 oz
12 PHY 375 Untreated FM 1773LLB2 Chris Main 6/8/2010
13 PHY 375 Ignite 29 oz/ac FM 1773LLB2 Chris Main 6/8/2010
14 PHY 375 Ignite 58 oz/ac FM 1773LLB2 Chris Main 6/8/2010
15 PHY 375 Ignite 87 oz/ac FM 1773LLB2 Chris Main 6/8/2010
16 PHY 375 Ignite 116 oz/ac FM 1773LLB2 Chris Main 6/8/2010
17 Plant Height 14 Days After B Treatment LSD = 3.3 PHY 375 FM 1773 LLB A AB C B D B AB AB C AB D BC BC E Check 1 app 2 app 29 oz 58 oz 87 oz 116 oz
18 Cotton Injury 28 Days After B Treatment LSD = ns PHY 375 FM 1773 LLB2 Check 1 app 2 app 29 oz 58 oz 87 oz 116 oz
19 Plant Height End of Season LSD = ns PHY 375 FM 1773 LLB2 35 Check 1 app 2 app 29 oz 58 oz 87 oz 116 oz
20 Maturity - NACB End of Season 7 LSD = 0.6 PHY 375 FM 1773 LLB C C B C A B AB C C C C C A C Check 1 app 2 app 29 oz 58 oz 87 oz 116 oz
21 Lint Yield End of Season LSD = A PHY 375 FM 1773 LLB2 A C BC BC BC BC C C BC C C C D Check 1 app 2 app 29 oz 58 oz 87 oz 116 oz
22 Tom Barber, Darrin Dodds, Chris Main
23 Variety Tolerance Varieties planted: FM 1735 LLB2 FM 1773 LLB2 FM 1845 LLB2 PHY 267 PHY 375 PHY 440 W PHY 499 PHY 565 Ignite Applications Untreated 2 29oz Applications Phyotoxicity 7, 14 DAA & DAB Plant Height, Nodes 14, 28 DAB, EOS Maturity NAWF (28 DAB) NACB (120 DAP) Gin turnout, Lint yield Fiber Quality
24 Variety x Ignite Parameter Time Parameter Time Parameter Time Injury 7 DAA Nodes 14 DAA Nodes EOS Injury 14 DAA Height 7 DAB NACB EOS Injury 7 DAB Nodes 7 DAB 1 st Pos.Ret. EOS Injury 14 DAB Height 14 DAB Ginout Harvest Injury 28 DAB Nodes 14 DAB Lint Harvest Height Initial Height 28 DAB Mic Harvest Nodes Initial Nodes 28 DAB Length Harvest Height 7 DAA NAWF 28 DAB Strength Harvest Nodes 7 DAA Ht:Node EOS Uniformity Harvest Height 14 DAA Height EOS
25 Cotton Injury 7 Days After A Treatment LSD = Check 2-29 oz Apps A 10 B B B B 5 0 C FM 1735 LLB2 PHY 367 PHY 375 Early Maturity C FM 1773 LLB2 PHY 440 W PHY 499 Mid Maturity C FM 1845 PHY 565 LLB2 Full Maturity
26 Cotton Injury 14 Days After B Treatment LSD = ns 25 Check 2-29 oz Apps FM 1735 LLB2 PHY 367 PHY 375 Early Maturity FM 1773 LLB2 PHY 440 W PHY 499 Mid Maturity FM 1845 PHY 565 LLB2 Full Maturity
27 Maturity - NAWF Days After B Treatment LSD = ns Check 2-29 oz Apps 0 FM 1735 LLB2 PHY 367 PHY 375 Early Maturity FM 1773 LLB2 PHY 440 W PHY 499 Mid Maturity FM 1845 PHY 565 LLB2 Full Maturity
28 Final Plant Nodes End of Season LSD = ns 19 Check 2-29 oz Apps FM 1735 LLB2 PHY 367 PHY 375 Early Maturity FM 1773 LLB2 PHY 440 W PHY 499 Mid Maturity FM 1845 PHY 565 LLB2 Full Maturity
29 Final Plant Height End of Season LSD = ns Check 2-29 oz Apps 30 FM 1735 LLB2 PHY 367 PHY 375 Early Maturity FM 1773 LLB2 PHY 440 W PHY 499 Mid Maturity FM 1845 PHY 565 LLB2 Full Maturity
30 Maturity - NACB End of Season LSD = ns FM 1735 LLB2 PHY 367 PHY 375 Early Maturity Check FM 1773 LLB oz Apps PHY 440 PHY 499 W Mid Maturity FM 1845 PHY 565 LLB2 Full Maturity
31 Lint Yield LSD = 103 Check 2-29 oz Apps * 1744* FM 1735 LLB2 PHY 367 PHY 375 Early Maturity FM 1773 LLB2 PHY 440 W PHY 499 Mid Maturity FM 1845 PHY 565 LLB2 Full Maturity
32 Conclusions ~15% Injury from 29 oz/ac applications Higher levels from overlaps Tank-mixes can increase injury Height reductions occur after application
33 Injury Comparison (7 DAA) Untreated Ignite 29 oz Ignite 58 oz Ignite 87 oz Ignite 116 oz
34 Conclusions Lint yield of WideStrike varieties can be reduced lb., but not always. WideStrike varieties yield greater than or equal to currently available Liberty Link varieties. More on ST 4145 LLB2 later
35 Disclaimer This presentation is not a recommendation to apply Ignite to WideStrike cotton varieties. Many applications in this presentation were above label rates, always check current labels for use directions. The University of Arkansas, University of Georgia, Mississippi State University and the University of Tennessee do not warrant the use of glufosinate ammonium herbicides on WideStrike cotton. Be willing to accept crop injury risks from applications of glufosinate ammonium herbicides to WideStrike cotton.
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