Automated Particle Size & Shape Analysis System

Transcription

1 Biovis PSA2000 Automated Particle Size & Shape Analysis System Biovis PSA2000 is an automated imaging system used to detect, characterize, categorize and report, the individual and cumulative particle data obtained from a sample. It offers a microscopy based digital image analysis solution for a comprehensive investigation of particulate matter. Particle size and shape details provide vital information regarding nature and quality of powdered materials. The particle size analysis system can be used in a wide range of applications, from Pharmaceutical and chemical industries, to Life science and Material science research labs, and in quality control applications. Different types of dry or wet particulate matter such as emulsions, crystals, powders, spray droplets, or suspensions can be analyzed by the system. The Biovis PSA2000 workstation is a complete Image analysis solution consisting of a Microscope with an attached high resolution digital camera, along with the Biovis Particles Plus software. This system is designed to furnish a simple and intuitive interface, with most of the operations carried out by built-in automation scripts thus avoiding much of the manual labour involved from users end. Automated solution for Measuring and Documenting Particle Size and Shape Particle analysis in accordance with certified standards Instantaneous results of particle counts & individual particle data Conserve time by automating recurring analysis tasks IQ/OQ Documentation 21 CFR Part 11 Compliance Advantages of Biovis PSA2000 : Calculate measurements on thousands of individual particles at the push of a button. Easily isolate different particle types on the basis of size, shape, color, etc. Pre-built analysis routines to automate the entire process workflow. Complete the entire procedure with a few mouse clicks. Allows users in labs and manufacturing facilities to save time and improve productivity. The Particle Size analysis and reporting is performed to meet compliance to FDA 21 CFR Part 11 Run unattended a batch of up to 500 images at a time and obtain consolidated statistical results. Store images and analysis results of your samples and experiments for archival and further reference. Generate customized analysis reports using pre-configured report templates

2 Image Acquisition and Processing High resolution digital microscope-camera directly integrated to the system Particles in the Image, as small as 0.5 micron can be easily detected and measured Rapidly detect thousands of individual particles Seamlessly integrated hardware requires minimal effort during analysis The high resolution digital microscope-camera ensures high accuracy Automated pre processing routines allow analysis of different sample types Optional motorized stage controller for rapid unattended scanning of multiple areas of the sample Individual Particle Measurements With a single click obtain total particle counts along with detailed size and shape information Automatic separation algorithms to separate touching particles Determine measurements such as area, length, perimeter, roundness, aspect ratio, etc. for each individual particle Choose from over 50 different types of measurements to be performed on the particles The measurement results on each particle can be viewed independently on screen Analysis Routines can be customized by the user depending on the sample and the application type Classification Optionally sort the detected particles into different classes based on any measurement criteria. Particles can be filtered by size, shape, color and other criteria and can be categorized into different groups. Results Reporting Save results of the Particle size analysis locally to text, MsWord, MsExcel documents. Create a formatted professional report of the analysis displaying the images, summary statistics, graphs, and with analysis timestamp. Automated Analysis Macro scripts can be used to automate the entire analysis operation. These scripts allow users to start & complete the analysis by a single click of the mouse. Batch Run Analysis Operate on a large batch of images sequentially without any user supervision. This can be used to setup a completely unattended analysis session on multiple Image samples.

3 LIST OF PARAMETERS MEASURED-P1 AREA AED Area % Area/Box Size (Length) Size (Width) Aspect Ration Axis (Major) Axis (Minor) Angle (Major Axis) Box Area Box (Length Box(width) Box (L/W) Area/Box area Centr-X Centr-Y Clusters Diameter(Av) Diameter(min) Diameter(max) Hole area Hole area ratio Perimeter Area of the object (excluding holes). If Fill holes in detected feature option from under the optional tab is selected then the Area includes the area of holes or voids within object Area equivalent diameter according to ISO Particle Size Standards, Square root of (4 X A)/, where A is area The Percentage area of the object with reference to the field area Area of object divided by the are of the minimum bounding box that could enclose the object The longest distance ( Maximum Length) between two opposite end point on the object in any direction The longest distance (Maximum Length) between two opposite end points on the object in the direction perpendicular to that considered for size(length) measurement. Ratio of size (Length) to size (width) The Length of the major Axis, of an equivalent ellipse to the object The Length of the minor Axis of an Equivalent ellipses to the object The Angle in Degrees between X axis and the major Axis of the object The area of minimum bounding box that can enclose the object The length of the minimum bounding box in the Y direction The width of the minimum bounding box in the X direction Ratio of the length to the width of the bounding box Area of object divided by the area of the minimum bounding box that could enclose the object The X coordinate of centroid of the object.(from the left side of the image) The Y coordinate of the centroid of the object (from the top of the image) If the object comprises of more than one object merged together, clusters indicate the number of objects merged to create this single Object. This parameter works well when the analyzed image consists of Objects having similar areas and shapes. The average of the diameters measured at equal intervals around the centroid of the object The minimum diameter or the shortest line between two perimeter points on the outline of the object and passing through its centroid The maximum diameter or the longest line between two perimeter points on the outline of the object and passing through its centroid If the measured object comprises of holes (areas that have an opposite intensity as that of the object) then the total area occupied by these holes alone is the Hole area. The measurement of Hole Area will be done only if the Fill holes in detected feature option is enabled (i.e., the Total Area of the object consists of even the hole area), else this value will be 0. The Hole area divided by the Total area of the object including holes. The measurement of Hole Area ratio will be done only if the Fill holes in detected feature option is enabled, else this value will be 0. The length of the outside edge or outline surrounding the object.

4 LIST OF PARAMETERS MEASURED-P2 Perimeter(convex) Radius(max) Radius(min) Radius ratio Mean Red Mean Green Mean Blue The length of the convex shaped outline that could enclose the object tightly like a rubber band, i.e., the minimum perimeter of a convex polygon enclosing the object. The maximum length between the objects centroid and the outside edge/outline The minimum length between the objects centroid and the outside edge/outline The ratio of the maximum radius to the minimum radius of each object The mean Red value of the object if the image is a color RGB image. The mean Green value of the object if the image is a color RGB image. The mean Blue value of the object if the image is a color RGB image. Density (mean) Mean intensity/optical density of the object. Reported in the units of the currently selected intensity calibration. Density (min) Minimum intensity/optical density of the object. Reported in the units of the currently selected intensity calibration. Density (max) Maximum intensity/optical density of the object. Reported in the units of the currently selected intensity calibration. Density(Integrated) Sum of Intensity/Optical density of each pixel in the object. Reported in the units of the currently selected intensity calibration. Density (Center X) The X coordinate of weighted density centroid of the object.(from the left of image) Density (Center Y) The Y coordinate of weighted density centroid of the object.(from the top of image) Density (mode) Intensity/Optical density pixel value which occurs most frequently in the object. Intensity (core) Intensity at the centre pixel/region of the object Distance (IP) Distance (IP) is the Inter Particle Distance. It is the minimum distance between the boundaries of the object and its nearest neighbor Circ. Factor Circularity factor is a measure of the roundness of the object using the Circ. Factor = ( pi * major axis * major axis) /( 4 * area) Roundness Roundness is a measure of the roundness of an object using the Roundness = (perimeter * perimeter ) / (4 * pi * area) For almost perfectly round objects, the Roundness value ranges from 0.8 to 1.2 Compactness Compactness is a measure of the compactness of the object using the Compactness= (perimeter * perimeter) / area

5 Elongation Roughness Perimeter ratio Perimeter/sq root area Perimeter/ Length Perimeter/ Width LIST OF PARAMETERS MEASURED-P3 Elongation is the ratio of the longest length to the longest width, which are both at right angles to each other. Roughness is a measure of the irregularity/roughness of the outline of the object calculated using the Roughness = perimeter/convex perimeter Measure of Shape factor of the object describing its roundness or elongation. Perimeter ratio = Perimeter of an equivalent circle having the same area as the feature / actual perimeter of the feature. Values close to 1 indicates a round object, values of < 0.2 describe worm like features. Perimeter divided by Square root of area Perimeter of object divided by its longest length Perimeter of object divided by its longest width Sq area/length Square root of the area of the object divided by its length Sq area/width Square root of the area of object divided by its width Thick (max) The maximum Thickness value measured from the object. Thickness is the distance between the principal surfaces of the object, as opposed to its length and width. Thick (mode) Skel. Length Class Range Cir. Equivalent Factor The Thickness value which occurs most frequently inside the object. The total skeletonized length of an object. If you imagine the object to be reduced to its skeleton structure by using the Skeletonize filter, then the total length of this skeleton dendrites gives the Skeletal Length. When classification has been performed, Class indicates the class number to which this object the object belongs The gray scale/intensity/color range label that this object belongs to. Area Equivalent perimeter/actual Perimeter