User Bulletin Applied Biosystems StepOne /StepOnePlus Real-Time PCR System

Transcription

1 User Bulletin Applied Biosystems StepOne /StepOnePlus Real-Time PCR System July 30, 2007 SUBJECT: Network Port Calibration and StepOne Instrument Connection Procedures In This User Bulletin How to Check that the Network Port of the Computer Is Set to DHCP This user bulletin covers the following topics: How to Check that the Network Port of the Computer Is Set to DHCP on page 1 How to Check that the StepOne Instrument Communicates with the Computer on page 6 If your computer does not connect to the StepOne instrument, you must check that the network port on the computer is set to DHCP (to automatically obtain the IP address). To check the status of your computer network port: 1. Disconnect your computer from the StepOne instrument and the Local Area Network. 2. Unplug all of the Ethernet cables from the computer. 3. From the desktop, select Start Control Panel.

2 .. Network Port Calibration and StepOne Instrument Connection Procedures 4. In the Control Panel window, double-click Network Connection. 5. In the Network Connections window, double-click the appropriate network port. 2 Applied Biosystems StepOne /StepOnePlus Real-Time PCR System User Bulletin

3 .. 6. In the Local Area Connection Properties dialog box, select Internet Protocol (TCP/IP), then click Properties. 7. In the General tab of the Internet Protocol (TCP/IP) Properties dialog box, select Obtain an IP Address automatically. Applied Biosystems StepOne /StepOnePlus Real-Time PCR System User Bulletin 3

4 Network Port Calibration and StepOne Instrument Connection Procedures 8. Select the Alternate Configuration tab, select Automatic private IP address, then click OK.. 9. From the Local Area Connection Properties, click Close. 10. Connect your computer to the StepOne instrument: a. Connect the yellow Ethernet cable to the yellow Ethernet port on the StepOne instrument. b. Connect the other end of the yellow Ethernet cable to the integrated Ethernet port on the computer, the same Ethernet port that corresponds to the Local Area Network connection configured in steps 5 through step Power on the StepOne instrument. Wait for the main menu to appear. 4 Applied Biosystems StepOne /StepOnePlus Real-Time PCR System User Bulletin

5 12. In the computer desktop, select Start Control Panel Network Connections. 13. In the Network Connections window, double-click the same network port as in step 5 on page 2. a 14. In the My Local Area Connection Status dialog box, select Support tab, then verify that the: a. Address type is Assigned by DHCP. b. StepOne instrument has sent an IP address to the computer. c. IP address begins with a b, c Applied Biosystems StepOne /StepOnePlus Real-Time PCR System User Bulletin 5

6 Network Port Calibration and StepOne Instrument Connection Procedures How to Check that the StepOne Instrument Communicates with the Computer You can now verify the connection between the computer and the StepOne instrument by pinging the StepOne instrument. To ping the StepOne instrument: 1. From the computer desktop, select Start Run to open the DOS window. 2. Enter cmd in the command line, then click OK. 3. After the DOS window opens, wait 15 sec. 4. At the DOS prompt, enter ping ,then wait for the response. 6 Applied Biosystems StepOne /StepOnePlus Real-Time PCR System User Bulletin

7 The following screen is displayed if the computer is communicating with the StepOne instrument. If there is no communication, take a screen capture of the screen on step 14 on page 5, then send it to your Applied Biosystems contact. Applied Biosystems StepOne /StepOnePlus Real-Time PCR System User Bulletin 7

8 Copyright 2007, Applied Biosystems. All rights reserved. Information in this document is subject to change without notice. Applied Biosystems assumes no responsibility for any errors that may appear in this document. APPLIED BIOSYSTEMS DISCLAIMS ALL WARRANTIES WITH RESPECT TO THIS DOCUMENT, EXPRESSED OR IMPLIED, INCLUDING BUT NOT LIMITED TO THOSE OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE. IN NO EVENT SHALL APPLIED BIOSYSTEMS BE LIABLE, WHETHER IN CONTRACT, TORT, WARRANTY, OR UNDER ANY STATUTE OR ON ANY OTHER BASIS FOR SPECIAL, INCIDENTAL, INDIRECT, PUNITIVE, MULTIPLE OR CONSEQUENTIAL DAMAGES IN CONNECTION WITH OR ARISING FROM THIS DOCUMENT, INCLUDING BUT NOT LIMITED TO THE USE THEREOF. The StepOne and StepOnePlus Real-Time PCR System are covered by patents owned by Applera Corporation. No right is conveyed expressly, by implication, or by estoppel to apparatus, reagents, kits, or methods such as the patented 5í nuclease methods. For further information, contact the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA. TRADEMARKS: Applera, Applied Biosystems, and AB (Design) are registered trademarks, and StepOne and StepOnePlus are trademarks of Applera Corporation or its subsidiaries in the U.S. and/or certain other countries. Microsoft and Windows are registered trademarks of Microsoft Corporation. All other trademarks are the sole property of their respective owners. Worldwide Sales and Support Applied Biosystems vast distribution and service network, composed of highly trained support and applications personnel, reaches 150 countries on six continents. For sales office locations and technical support, please call our local office or refer to our Web site at Applera is committed to providing the world s leading technology and information for life scientists. Applera Corporation consists of the Applied Biosystems and Celera Genomics businesses. Headquarters 850 Lincoln Centre Drive Foster City, CA USA Phone: Toll Free (In North America): Fax: / Part Number Rev. A Stock Number 117UB29-01