Grape powdery mildew fungicide Trial 2, a. Trial layout and method
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1 Grape powdery mildew fungicide Trial, 0 Location Principle investigator Research associates Cooperators Crop Disease Herzog Ranch, near Courtland, Sacramento Co., California Doug Gubler, Ph.D. Ken Asay, Chris Janousek, Doug Gubler John, Cathy and Randy Baranek, Tom Herzog Grape, Chardonnay variety Powdery mildew (Uncinula necator) Objective Experimental design a. Trial layout and method Test the efficacy of fungicides for control of powdery mildew. Treatments are field applications to vine plots, in a randomized complete block design, with replicates. High pressure hand gun sprayers, backpack sprayers. Application method Vine spacing ft Row spacing ft Treatment unit vines Treatment unit area ft Area/Treatment ft Area/Treatment 0.0 acres Volume water/acre 0 gallons gallons gallons Volume water/treatment.0 gallons.0 gallons. gallons Application frequency Variable Evaluation stage Veraison b. Fungicide treatments Trt. No. Flag Institution Materials Application W Untreated control UC Davis Flint alt AE KD Sovran alt BF Quintec alt CG Procure alt DH Interval FP/acre FP/Treatment - RI. oz alt. oz alt. fl oz alt. fl oz alt. g alt. g alt. ml alt. ml alt YS Crompton Procure 0SC AB. fl oz. ml YKS YD Crompton Procure 0SC alt Flint Gowan (Pristine + Latron B- ) then (Vintage + Latron B-) AC BD B C D E - then -. fl oz alt. oz. oz. oz. oz. oz. ml alt. g. g. ml. ml. ml Notes + ml/acre Latron B-
2 Trt. No. Flag Institution Materials Application Gowan (Pristine + Latron B- B ) then C (Rubigan EC + D Latron B-) E OKS YRD AgraQuest Flint 0WG alt (Sonata ASO + Silwet L-) AC BD Interval FP/acre FP/Treatment -. oz. g then. fl oz. ml -. fl oz. ml. fl oz. oz alt (. qt + ml). ml. g alt (. ml +. ml) OS AgraQuest Quintec.0 SC alt (Sonata ASO + Silwet L-) AC BD. fl oz alt (. qt + l). ml alt (. ml +. ml) RC EcoSmart Sporan EC AB. pt. ml 0 GS RD LG BS EcoSmart Isagro- Ricerca USA Isagro- Ricerca USA Isagro- Ricerca USA Sporan EC alt AC.... pt alt. ml alt Quintec BD.... fl oz. ml Eminent SL AB - RI. fl oz. ml Eminent SL AB - RI. fl oz. ml Eminent SL AB - RI. fl oz. ml OD Bayer USF0 0 WG AB. oz. g GD Bayer Elite WP alt AC.... oz. g Flint 0WG BD.... oz. g RKC Syngenta JMS Stylet Oil A Abound BC. fl oz 0. ml P DuPont DPX LEM SC AB -0. oz ai. ml Pu DuPont DPX LEM SC AB -0. oz ai. ml YC DuPont DPX LEM SC AB -0. oz ai ml YKC DuPont DPX LEM SC AB -0.0 oz ai. ml GKD DuPont Endura 0WG or -0. oz or.0 g or Sovran. oz.0 g KS DuPont Quintec.0SC AB -0. fl oz. ml RKS DuPont KQ AB -0. fl oz. ml OKD DuPont DPX LEM alt AC -0. oz ai. ml KQ BD. fl oz. ml KC Natural Micro 0 + AB. oz. g Industries Silwet L- ml. ml Notes + ml/acre Latron B- Note: The treatments described in this report were conducted for experimental purposes only and crops treated in a similar manner may not be suitable for commercial or other use. FP = formulated product; ai = active ingredient; alt = alternated with; RI = Gulber-Thomas powdery mildew risk index.
3 c. Fungicide information Institution Product Active Ingredient(s) Concentration(s) Contact UCD JMS Stylet Oil mineral oil % Flint tryfloxystrobin 0 % Sovran kersoxim methyl 0 % Quintec quinoxyfen 00 g/l UCD & Procure 0SC triflumizole 0 g/l Crompton Gowan Pristine pyroclostrobin. % James Brazzle boscalid. % jbrazzle@gowanco.com Vintage fenarimol. % Rubigan EC fenarimol lb/gal Latron B- non-ionic surfactant % AgraQuest Flint 0WG trifloxystrobin 0 % Paul Walgenbach Sonata ASO Bacillus pumilus. % pwalgenbach@agraquest.com Silwet L- silicone surfactant 00 % EcoSmart Sporan EC rosemary oil % Ramon Georgis clove oil 0 % rgeorgis@ecosmart.com thyme oil 0 % Quintec quinoxyfen 00 g/l Isagro- Ricera Eminent SL tetraconazole. % Chris Leon cleon@isagro-usa.com Bayer USF0 0WG tebuconazole % Matt Ehlhardt trifloxystrobin % matt.ehlhardt@bayercropscience.com Elite WP tebuconazole % Flint WG trifloxystrobin 0 % Syngenta Abound azoxystrobin 00 g/l Tim Tripp DuPont DPX LEM unknown % SC (=DPX LEM 0) Endura 0WG boscalid 0 % Quintec.0SC quinoxyfen 00 g/l KQ proquinazid 0 g/l Natural Micro 0 Streptomyces lydicus 0 CFU/g Industries Silwet L- silicone surfactant 00 % tim.tripp@syngenta.com Stephen Colbert stephen.f.colbert@usa.dupont.com Tim Lichatowich tim@naturalindustries.com
4 d. Fungicide applications Date May 0 May 0 May 0 May 0 May 0 Vol 0 gal/acre 0 gal/acre 0 gal/acre 0 gal/acre 0 gal/acre X X X X X X X X 0 X X X X X X X X X X X X X X X X X X X X X X X X X
5 Date May 0 May 0 May 0 May 0 May 0 Stage Vol 0 gal/acre 0 gal/acre 0 gal/acre 0 gal/acre 0 gal/acre X X X X X X X X 0 X X X X X X X X X X X X X X X X X X X X X X X X X
6 Date June 0 June 0 June 0 June 0 June 0 Stage Pea-sized grapes Vol 0 gal/acre 0 gal/acre gal/acre 0 gal/acre 0 gal/acre X X X X X X X X X X X X 0 X X X X X X X X X X X X X X X X
7 Date June 0 June 0 June 0 June 0 June 0 Stage up to small marble Small marble sized sized grapes Vol 0 gal/acre 0 gal/acre 0 gal/acre 0 gal/acre gal/acre X X X X X X X X 0 X X X X X X X X X X X X X X X X X X X X X X X
8 Date 0 June 0 July 0 July 0 July 0 July0 Vol gal/acre gal/acre gal/acre gal/acre gal/acre X X X X X 0 X X X X X X X X X X X X X X X X X X X X X X X
9 Date July 0 July 0 July 0 Vol gal/acre gal/acre gal/acre X X. ml Vintage X. ml Rubigan X X 0 X X X X X X X X X Additional notes on applications: May, 0: Disease spotted on one end vine within the trial. - May: Most products applied at % of acreage rate. May : Disease spotted in one control vine leaves. May : Disease moving into controls. 0 % leaf infection, no fruit infection. June : Disease on untreated leaves 0%, on fruit %. June : Treatment - Both Flint and Elite were sprayed on the same day. June : Began manual thinning of vines, which lasted one week. June : Spray volume was increased from 0 gal/acre to gal/acre. July : Silwet L- sticker increased to. ml (per plots) for treatments,, and.
10 DPX-LEM SC and KQ applications Fungicide Treatment Dates fungicide applied May June July DPX-LEM at -0 day X X X X X X X X X X. DPX-LEM at -0 day X X X X X X X X X X. DPX-LEM at -0 day X X X X X X X X X X. DPX-LEM at -0 day X X X X X X X X X X. DPX-LEM at -0 day alt X X X X X KQ at -0 days X X X X X. KQ at -0 day X X X X X X X X X X Vintage, Rubigan and Abound application frequency Fungicide Treatment Pristine (.g) + Latron B-, - d then X.ml. ml. ml Vintage, - d + Latron B- sticker X X. Pristine (.g) + Latron B-, - d then Rubigan on - day + Latron sticker (.0ml) X.ml. ml. ml TRIAL RATING TRIAL RATING Dates fungicide applied May June July. JMS Stylet Oil then X X X Abound at 0.ml on day X X X X X PM Risk Index
11 Endura applications Fungicide Treatment Dates fungicide applied May June July Endura (.oz/acre) on -0 day and Sovran (. oz/acre) on -0 day cycle X X X X X X X X X X Time lapsed since last application (d): 0 Eminent applications Fungicide Treatment fl oz/acre at - days.. fl oz/acre at - days.. fl oz/acre at - days X X X X X RATING RATING Dates fungicide applied May June July X X X X X X X X X X Time lapsed since last application (d) PM Risk Index
12 e. Plot map Trial V then Dirt Road Row Row Row Row Row Row 0 Row Row X X X X X X X X W X BS GS KD X KC X KC OKD OD LG OKD YD GS GD YC KD GD RD GS RC W KS P OKS RKC X P GD P YKC Pu OS P RC RD W RKC BS BS RD Pu OS BS OD LG OS YKS RC YC YRD YKC LG YC RD RKC GD YKC OKS OS YRD KD YRD GS LG GKD YD RKC YC OKD RKS YRD RKS KS YKS KC RKS RC YD YD KS RKS YS KS Pu GKD OKS GKD OD OKD KD YS GKD OD YKS YS YKC KC W YKS OKS YS Pu X X X X X X X X Block Block Block Block Grass Dirt Road Waterway X = vines not used in the experiment.
13 f. Herzog Ranch 0 PM risk index All Risk Index data from: Risk Index (%) May -May -May -May 0-Jun -Jun 0-Jun 0-Jul -Jul 0-Jul Day/Month in 0
14 g. Results Table. Trial mean powdery mildew severity (± S.E.). Non-significant groups of means are represented by the same letter (Tukey-Kramer test). All treatments consisted of replicates. Significance Treatment description Disease severity groups (at p<0.0) Untreated control. (±0.) a Sporan EC oil, days,. pints/acre. (±.) b Micro 0 + Silwet L-, days,. oz/acre. (±.) bc Eminent, - days,. fl oz/acre. (±.) bcd Eminent, - days,. fl oz/acre. (±.) bcd Pristine then Vintage, - then - days. (±.) cde Sporan alt Quintec, d,. pts/acre alt. fl oz/acre. (±.) cdef Procure, days,. fl oz/acre.0 (±.) cdefg Flint alt Sovran alt Quintec alt Procure, - days. (±.) defg Elite alt Flint, alt days. (±.) defg DPX LEM SC, -0 days,. oz ai/acre. (±.0) defg Pristine then Rubigan, - then - days. (±.) defg Eminent, - days,, fl oz/acre. (±.) defg Procure alt Flint, days,. fl oz/acre alt. oz/acre. (±0.) defg USF0, days,. oz/acre. (±.) defg Quintec alt Sonata ASO + Silwet L-, days. (±.) defg Endura, Sovran mix, -0 days,. oz/acre. (±.) defg Abound, days,. fl oz/acre. (±.) defg Flint alt Sonata ASO + Silwet L-, days. (±.) efg DPX LEM SC alt KQ, -0 days. (±0.) efg KQ, -0 days,. fl oz/acre. (±0.) fg DPX LEM SC, -0 days,. oz ai/acre. (±0.) fg DPX LEM SC, -0 days,.0 oz ai/acre 0. (±0.) g DPX LEM SC, -0 days,. oz ai/acre 0. (±0.) g Quintec, -0 days,. fl oz/acre 0. (±0.) g
15 Figure. Mean per-vine grape yield (± S.E.) in selected treatments from trial. There was no significant difference between treatments (Type III, factor ANOVA: p>0.). a a a a Yield (kg grapes/vine) 0 Control DPX LEM SC,. fl oz/acre DPX LEM SC,.0 fl oz/acre KQ,. fl oz/acre Fungicide treatment h. Conclusions Powdery mildew severity was reduced in all treatments relative to unsprayed controls. All -0 day treatments reduced powdery mildew cover below %. Several other products reduced PM severity to <0%, including Abound and USF0. Dosage dependent-effects of DPX LEM SC and Eminent fungicides on powdery mildew severity were implicated by the data, but because of low replication, a posteriori Tukey-Kramer comparisons did not show significant differences. For the subset of treatments investigated, there was no difference in fruit yield between control plots and sprayed plots. Copyright 0, 0 The Regents of the University of California, Davis campus. All rights reserved.
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