NIKON TE2000E Microscope: 2. Objectives: 3. CCD Cameras: PCO Sensicam qe

Transcription

1 NIKON TE2000E Microscope: Compatible for all advanced live cell applications, this advanced inverted research station was conceived to provide the highest level of optical imaging for today s competitive research scenes. The TE2000-E model incorporates a high precision motorized-focus and vibration-free optical path changeover mechanism that facilitates image capture in 3D. Multi-port design: The E model has five ports. Depending on the research techniques you are using, you can easily integrate various pieces of imaging equipment in your desired combination and placement. Optional stage risers create space for other attachments: Taking advantage of infinity optics, the TE2000 s extendible design allows the distance between the objective and tube lenses to be extended up to 80mm (max.). This feature enables the researcher to add optional attachments without modifying the microscope. For example, by using optional stage risers you can add a laser light source, without affecting the performance or stability for standard epi-fluorescence or DIC techniques. Motorized-control adaptability: The TE2000 is designed to accept retrofitable motorized options in major sections of the microscope, which allows the researcher to choose the desired combinations via the dedicated communications hub controller to suit the required research method. Unprecedented signal to noise ratio by eliminating stray light: The new Noise Terminator technology directs deviated stray light out of the objective light collection path. This results in images of high contrast and unparalleled S/N ratio during fluorescence observation using advanced techniques such as evanescence wave microscopy (TIRF) increasing the contrast and extending the detection level limit. CFI60 optical system: The TE2000 adopts CFI60 infinity optics, known for crisp, clear images at any magnification, while providing higher N.A. s and longer working distances. Stability for greater precision: Nikon implemented CAE analysis and adopted a new high-strength alloy material to produce a main body structure, in which minor ambient temperature fluctuations do not adversely affect the image to be captured. Greater Z-axis precision: The model TE2000-E features a motorized focus that is precisely controlled by high-precision Z-axis readout. This feature is perfect for research that requires comprehensive 3D information about the specimen. Auto Descending Nosepiece: The TE2000-E is provided with a mechanism, in which, when changing the objective magnification the nosepiece automatically decends and returns to the original height after the rotation finished. This design permits the researcher to rotate the nosepiece easily and safely to change the objective magnification. 2. Objectives: CFI S Fluor 10X N.A. 0.50, W.D. 1.2 mm( 超级荧光物镜 )Spring-loaded CFI S Fluor 20X N.A. 0.75, W.D. 1.0 mm( 超级荧光物镜 )Spring-loaded CFI S Fluor 40X N.A. 0.90, W.D. 0.3 mm( 超级荧光物镜 )Spring-loaded, Cover glass correction: mm CFI S Fluor 40X Oil N.A. 1.30, W.D mm( 超级荧光物镜 )Spring-loaded CFI S Fluor 100X Oil N.A. 1.30, W.D mm 超级荧光物镜 )Spring-loaded CFI Plan Fluor 60X Oil with Iris diaphragm N.A. 1.25, W.D. 0.20mm ( 平场荧光物镜 )Spring-loaded CFI Plan Fluor DL10X N.A. 0.30, W.D mm, Ph1( 平场荧光相差 ) CFI Plan Fluor ELWD ADL 20XC N.A.0.45, W.D mm( 平场荧光切趾相差物镜 )Spring-loaded, Cover glass correction:0-2.0mm CFI Plan Fluor DLL 40X N.A. 0.75, W.D mm, Ph2( 平场荧光相差物镜 )Spring-loaded CFI Plan Fluor ELWD ADL 40XC N.A.0.6, W.D mm( 平场荧光切趾相差物镜 )Spring-loaded, Cover glass correction:0-2.0mm CFI Plan Fluor DLL 100X Oil N.A. 1.30, W.D. 0.2 mm, Ph3( 平场荧光相差物镜 )Spring-loaded, Stopper 3. CCD Cameras: PCO Sensicam qe: This high performance cooled digital 12 bit CCD camera system comprises advanced CCD and electronics technology. The system features thermo-electrical cooling of the image sensor (down to 12 C), extremely low noise (down to 4 e- rms) and an outstanding quantum efficiency, which achieves a high spectral sensitivity in general and especially in the NIR. Exposure time modes (software selectable) range from 500 ns (fast shutter) to 1000 s (long exposure). A high speed serial data link connects the system to the PC (fiber optic link available). Areas of application: laser induced fluorescence, fluorescence microscopy, electron microscopy, red & NIR fluorescence applications, bioluminescence / chemoluminescence, spectroscopy, gel imaging, ion imaging, low light level imaging, semiconductor quality control, imaging of bio markers (e.g. GFP)

2 unit setpoint sensicam qe resolution (hor x ver) pixel 1376 x 1040 pixel size (hor x ver) µm² 6.45 x 6.45 peak quantum efficiency nm typical 62 dynamic range A/D bit 12 readout noise e- high/@gain low 4..5 / 5..6 imaging frequency, frame rate frame/@binning 2x2 10 / 19.8 exposure time s 500ns..1000s binning horizontal 1,2,4,8 binning vertical 1,2,4,8,16,32 optical input C-mount, Nikon adapter data interface PCI local bus, Rev. 2.1 cooled CCD temperature C -12 Roper Cascade 512B: The Cascade:512B digital imaging system from Photometrics offers very high sensitivity through the use of on-chip multiplication gain. A back-illuminated 512x512-pixel array (16x16-um pixels) enhances this sensitivity, while providing outstanding quantum efficiency and good field of view. The 16-bit, thermoelectrically cooled system can be operated at 10 MHz for high-speed image visualization or more slowly for high-precision photometry. Supravideo frame rates are achievable via subregion readout or binning. The camera can be configured with dual amplifiers to ensure optimal performance not only for applications that demand the highest available sensitivity (e.g., GFP-based single-molecule fluorescence) but also for those requiring a combination of high quantum efficiency and wide dynamic range (e.g., Ca 2+ ratio imaging). Features Benefits On-chip multiplication gain Very high sensitiveity, Low-noise, impact-ionization processs Back-illuminated CCD Highest available quantum efficiency (>90% peak QE) 512x512 imaging array 16x16-um pixels Good field of view and semsitivity, Good resolution 10-MHz readout 5- and 1-MHz readout Excellent for high-speed imaging visualizeation, Perfect for high-precision photometry Dual amplifiers Select readout mode via software for optimized (1) high-speed/high-sensitivity performance or (2) wide-dynamic-range performance 16-bit digitization Wide dynamic range allows detection of bright and dim signals in the same image Frame-transfer CCD 100% duty cycle to collect continuous data, No mechanical shutter required Thermoelectric cooling Reduces background for high sensitiveity C-mount Easily attachés to microscopes, standard lenses, or optical equipment Acquisition software Captures, analyzes, and saves high-resolution images PCI interface High-bandwidth, uninterrupted data transfer PVCAM Supported by numerous third software packages Circular buffers Real-time focus Device sequencing Precise integration with shutters, filter wheels, etc. Specifications CCD image sensor CCD format Linear full well Digitizer type Read noise On-chip multiplication gain e2v CCD97; back-illuminated, frame-transfer CCD with on-chip multiplication gain 512x512 imaging pixels; 16x16-um pixels; 8.2x8.2-mm imaging area (optically centered) Single pixel 200 ke- Output node 800 ke- ( on-chip multiplication gain amplifier) MHz, 5MHz, and 1 MHz On-chip multiplication gain amplifier (Port#1): ~45 e- 5MHz, ~60 e- 10 MHz, Read noise effectively reduced to < 1 e- rms with on-chip multiplication gain enabled Traditional amplifyier (Port#2): ~10 e- 1 MHz, 15 e- 5 MHz 1 to 500x (gurarnteed), 1 to 1,000x (typical), Software controlled in 4,096 steps

3 Parallel (vertical) shift rate CCD temperature Dark current Binning Operating environment 2.0 usec/raw -30 C (regulated) 1.0 C (0.5 C typical) Flexible binning capabilities in parallel direction; 1 through 6 binning in serial direction 0 to 30 C, 0 to 80% relative humidity noncomdensing 4. Till Polychrome IV: The Polychrome IV is a fourth-generation, rapidly-tunable excitation source from TILL Photonics. The Polychrome IV is comprised of a xenon light source, a galvanometric scanner, mount grating, and mirror optics. The monochromatic light is coupled to specially designed epi-fluorescent condensers via a solid quartz fiber. The specially designed condensers are available for most microscopes and provides for optimal illumination. High Output: Unlike other monochromators and wavelength switchers, the Polychrome IV delivers a bright (6-8mw), evenly illuminated field at the objective with a bandwidth of 15nm and a homogeneity of better than 5%. High Speed: The Polychrome IV has the ability to jump to any wavelength between 250 and 680nm in less than 3ms, and provides a remarkable 1.5ms Fura jump between 340 and 380nm. Easy to Upgrade: The modular design of the Polychrome IV allows components to be easily added for complete imaging and photometric systems as well as uncaging flashes, D/A converters and other accessories. In fact, TILL Photonics has complete systems available for a wide range of applications including photometry, imaging, and uncaging. Specifications: Output Range Approximately 250nm to 650nm Output Power 6-8mw at object field from 340nm to 660nm Output Bandwidth 15nm typical, optional manual adjustment of output slits to provide 12, 10, & 8nm Light Homogeneity Uniform within 5% throughout the object field when properly adjusted for 'critical' illumination Response Time Less than 1.5ms for a 340nm to 380nm step. Less than 3ms for a 300nm to 700nm step Flexibility Coupling to practically any microscope via a 1.25mm x 1.8m solid quartz fiber (0.25na) and custom condenser assemblies available for most common research grade microscopes Control RS-232 serial or direct analog control via ±10 vdc Lamp Type 150 watt xenon short arc lamp Lamp Life 3000 hours Lamp Usage Lamp should be cycled on and off in not less than 30 minute intervals Power Consumption 120 watts operating (600 watts starting) Size: Control Unit 14" wide, 6" tall, 12" deep; Monochromator Cube 14" wide, 8" tall, 6" deep Typical Factory Calibration Specifications: Output Range Minimum Wavelength 248nm Maximum Wavelength 682nm 5. Dual-View for fluorescence imaging (Optical Insights): Simultaneous acquisition of 2 fluorescence emission images in a single snapshot Easily mounted to any microscope and CCD Uses standard 25 mm diameter emission and polarization filters Removable filter cube makes configuration for different experiments a snap Pass-Thru option means you can leave system in place for full field-of-view imaging Precision optical and mechanical design allows for sub-pixel image registration and minimizes light loss Real-time dual-color imaging CFP/YFP and GFP/RFP Fluorescent Resonance Energy Transfer (FRET) imaging Calcium imaging with Fluo3/Fura Red or dual emission Indo-1 imaging Simultaneous Calcium/pH imaging w/fura-2 and BCECF Fluorescence polarization/anisotropy imaging Drug discovery with Cy3/Cy5 Single Molecule Fluorescence (SMF) ph imaging with SNARF Multi-wavelength TRIF Multi-wavelength FLIM

4 Voltage sensing ph Imaging with SNARF FISH Cyclic AMP 6. Inverted BioPrecision stepper motor stage (Ludl Electronic): The LEP inverted microscope stages are designed to compliment and enhance the capabilities of most research inverted microscopes. The 121mm x 102mm travel stages are very well suited to the wide array of applications that make the inverted microscope so versatile. A full range of specimen holders such as multi-well plates, flasks, Petri dishes and standard 1 x3 and 2 x3 glass slide holders further enhance the flexibility of the microscope. Specifications: 121x102 mm travel range; 0.05um minimum resolution; <1um repeatability; 6um absolute accuracy; Maximum speed 60mm/sec. (stepper motor); Thermal compliant design is suitable for environmental chambers; All parts anodized for long lasting finish MAC 5000 Control System: The MAC 5000 control system provides high performance motion control for all LEP XY stage. The unique, patented stacked modular design allows for the system to be configured for almost any application. The LEP design philosophy makes the MAC 5000 system easy to integrate, simple enough for basic applications, yet sophisticated and powerfull enough for very advanced applications. 7. MetaMorph and MetaFluor Imaging system: The MetaMorph Imaging System is a powerful and affordable integration of software and hardware from Universal Imaging Corporation that enables scientific researchers to automate image capture and analysis from scientific grade digital CCD cameras. Bioimaging techniques contribute to a growing number of scientific breakthroughs. MetaMorph from Universal Imaging Corporation plays a large role in this revolution. With its image acquisition, processing, and analysis capabilities, and complete set of tools for automation, MetaMorph opens the door for new insights into cellular function. MetaMorph s flexibility and versatility make it a powerful system for performing operations such as time lapse, multi-dimensional acquisition, and 3D reconstruction, and for making measurements such as morphometry, colocalization, and brightness. In biological experiments using live cell imaging, MetaMorph combines the speed, flexibility, and unmatched customer support required to get better results, faster. MetaMorph provides high-end control for devices including microscopes, filter wheels, shutters, cooled CCD cameras, video cameras, monochromators, focus motors and Piezo electric focus devices, motorized stages, digital and serial input/output, and robotic devices. Fluorescence ratio imaging involves the introduction of a fluorescent indicator into living cells to allow the monitoring of these cells through a microscope using a photodetector. In imaging, the detector is a sensitive digital or video camera. Indicator dyes have been designed which shift their fluorescence excitation or emission spectrum upon binding the ions of interest. Images are obtained at two appropriate wavelengths, typically matching the absorption maxima at the high and low binding conditions. By ratioing the intensities in the image at corresponding picture elements, a map can be constructed showing the local ion concentrations throughout the field of view. Since the monitoring process is nondestructive, image acquisition can be repeated frequently to trace out the time course of cellular responses. Designed for single or dual wavelength intracellular ion measurements, MetaFluor supports FURA-2, BCECF, INDO-1 and other common ratiometric indicators. For easy viewing and analysis, MetaFluor provides simultaneous display of the original wavelengths, the ratio images, graphs of intensities, ratios, and ion concentrations, and a non-ratiometric image such as a brightfield or phase-contrast image. Two different ratiometric indicators can be imaged and measured at the same time. Computer controlled shutters minimize cellular exposure to the excitation illumination, reducing photo damage to your sample. MetaFluor supports a variety of image storage devices for archiving original image data, facilitating post-experiment processing and analysis or the use of an auxiliary, analysis-only workstation. MetaFluor's flexible design offers the benefit of tailoring your system configuration to your specific application. This versatile architecture enables you to upgrade the system as new hardware, such as wavelength selectors and cameras, become available. For example, your current application may require a video rate camera, but future applications may benefit from the high precision of a cooled CCD. MetaFluor's modular design makes it easy to take advantage of new technology as it becomes available.

5 MetaMorph and MetaFluor Imaging systems are a powerful integration of software and hardware that enables scientific researchers to automatically capture microscopy or macro images from scientific grade digital and video CCD cameras, providing the researcher flexibility in analytical capability. Developed in conjunction with leading bioscience researchers, the systems offer a variety of tools for imaging applications such as: 3D Deconvolution; 3D Reconstruction; Automated Microscopy; Brightness Measurements; Cell Counting; Colocalization; Confocal Support; Deconvolution; Densitometry; Digital Haze Removal; Digital Photography; Enhanced Contrast Microscopy; Fluorescence Overlay; Fluorescence in situ Hybridization (FISH); Fluorescence Immunocytochemistry; Fluorescence Recovery After Photobleaching (FRAP); Fluorescence Resonance Energy Transfer (FRET); Fluorescence/Green Fluorescent Protein (GFP); Gene Chips & Microarrays; Industrial Applications; Live/Dead Assays; Morphometry; Motion Analysis & Particle Tracking; Multi-Dimensional Imaging; Multiwell Plates; Neurite Outgrowth; Ratio Fluorescence; Screening; Stitching; Tissue Microarrays; Time Lapse; Z-series