New Olympus FV3000RS Laser Scanning Confocal System. Quotation Number V2
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1 New Olympus FV3000RS Laser Scanning Confocal System Quotation Number V2
2 Quotation Date: 08/08/2018 Revised Date: 07/09/2018 Quotation number: Quotation Expiry: 07/11/2018 Macquarie University Department of Molecular Sciences Dear Dr. Lindsay Parker Olympus FV3000RS Quotation Olympus FV3000RS Laser Scanning Confocal Microscope System is composed of: Scanning and Detection: o New FV3000RS System with Hybrid Scanning Unit: 1 silver-coated resonant and 1 silver-coated galvanometer scanning mirrors. Coupled with the fastest Resonance Scanner on the market (ability to capture up to 438fps at 512 x 32; 30fps at 512 x 512 at FN18) o Ability to switch between galvanometer and resonance scanning at the touch of a button; o Olympus Super Resolution: 2 Fluorescent signals simultaneously (max. 8 channel sequentially) with a resolution of 120nm. o New 4CH TruSpectral detection Method: 2CH High Sensitivity GaAsP Detectors and 2CH PMT Detectors.
3 o Max. 16 virtual channel function with spectral deconvolution/unmixing o 1 Transmitted Light PMT Detector and LED conventional illumination for LSM and TL Nomarski DIC Observation. Users can undertake simultaneous multichannel confocal fluorescence imaging and transmitted DIC acquisition. Lasers: o 4 Laser Diodes 405nm (50mW), 488nm (20mW), 561nm (20mW) and 640nm (40mW). * 980nm laser (collimator, fiber and adaptor) is not included in this quote. o Beam axis combiner unit and DM holder BDMOPT which combines 980nm laser beam onto FV3000RS. o IO interface box which outputs Digital signal for laser shuttering and analog signal for volume control of 980nm laser. o 10% or 100% maximum laser power changer by ND filter. Microscope & Optics: o Inverted IX83 2 Deck system Microscope Frame high thermal rigidity and stability due to its design reduces the impact of heat and vibration and improves quality of time-lapse imaging. o IX3-SSU Ultrasonic Piezo XY Motorised Stage with 4 types of holders: including an universal hold designed for non-standard chamber slide or dish; o Olympus IX3-ZDC2 Zero Drift Control Module for Live Cell Time Lapse Experiments
4 o Olympus Silicon Oil Objective 30X for excellent performance for deeper tissue imaging with a wider field of view. Distortion-free imaging in thick tissues or stem cells, NA: 1.05 (silicone oil immersion), W.D.: 0.8 mm o Olympus Silicon Oil Objective 60X for excellent performance during live cell Imaging. High resolution and long term time lapse imaging of single cells, NA: 1.30 (silicone oil immersion), W.D.: 0.3 mm o Olympus UAPON100XOTIRF NA1.49, TIRF objective lens, suitable for high resolution imaging of cell membranes or subcellular organelle and single molecule level experiments; suitable for super resolution imaging. o Olympus UCPLFLN Objective 20X, designed as large field of view, high NA and long working distance to optimize images through different cell culture vessels (suitable for both glass- or plastic- bottom vessels). NA: 0.7, W.D.: mm with correction collar. * UPLSAPO 10X, 20X, 40X, 60X and 100X objective lens on current FV1000 will be reused. o Nomarski Differential Interference contrast capability on 10X, 20X, 30X, 40X, 60X, 100X objectives: DIC rings for 30X and for 100X * DIC rings for 10X, 20X, 40X and 60X and DIC slider on current FV1000 will be reused. o Illumination for Widefield Observation, fluorescence filters included: WU2 BP BA420 WBV2 BP BA475 NIBA3 BP BA WIG3 BP BA575 IF * PE-300 Lite LED light source and FL filters on current FV1000 will be reused. Anti-vibration table and System Housing: o M-VIS3636-SG2-325A Vision IsoStation Antivibration Table: 900 mm x 900 mm. o Server Rack to house Power Supply components. CO2 Incubation for long term live cell imaging: o On stage or Full Enclosure Tokai Hit on Incubator with CO 2 and temperature control: full sample holder package for Wellplate, 35mm dish and chamber slide. Acquisition Work Station: o HPZ4G4 Olympus Tested Imaging PC (64GB RAM) and 32 monitor Software: o New FV3000 Software full package with Ease of use customization per user including: o Multiple Area Time Lapse acquisition, o Microplate Navigator for Live Cell Multi-positon Imaging, suitable for types of Microplates or Chamber slides; o VCH functions with 16CH sequential scanning; o Precise Sequence Manager and Real-Time Acquisition: Complex protocols are handled with ease, and real-time control;
5 o 3D Visualization and Observation: Interactive volume rendering display, projection display, animation display; o 3D animation (maximum intensity projection method, α blending) 3D and 2D sequential operation function; o Spectral Unmixing; o Life Science Analysis: Count and Measure, Colocalization, FRET, FRAP and etc; o 2D deconvolution and 3D deconvolution (constrained iterative deconvolution with GPU processing). Reviewer Work Station for data analysis: o HPZ4G4 Olympus Tested Imaging PC (64GB RAM) and 32 monitor; o Full Desktop License of FV3000RS with tiling function; o 3D Visualization and Observation: Interactive volume rendering display, projection display, animation display; o 3D animation (maximum intensity projection method, α blending) 3D and 2D sequential operation function; o Spectral Unmixing; o Life Science Analysis: Count and Measure, Colocalization, FRET, FRAP and etc; 2D deconvolution and 3D deconvolution (constrained iterative deconvolution with GPU processing). Olympus FV3000RS Systems Price $299,950 ex GST Additional Information For a full list of Terms and Conditions of Sale please visit the Olympus Australia website at Pricing: All Prices quoted in AUD and ex GST. Warranty: 12 Month Manufacturer s Warranty included in this offer on all Olympus parts. 24 Month Manufacturer s warranty on PC unit and monitors. Delivery and Installation: Delivery to installation site with full installation. Estimated Delivery Date: 8-12 weeks for all Olympus parts. Training: Total 3 day Onsite training provided by the Olympus Applications Specialist. Training provided as 2-3 sessions. Basic training at installation and more advanced training at a later mutually agreed time. Should you have any further enquiries, please do not hesitate to contact us.
6 We look forward to discussing this grant proposal with you in more detail. Yours Sincerely, OLYMPUS AUSTRALIA Tong Wu Microscopy Sales Specialist Kim Everuss Sales Manager SSBU
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