Supplement of Quantifying environmental stress-induced emissions of algal isoprene and monoterpenes using laboratory measurements
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1 Supplement of Biogeosciences, 12, , doi:1.5194/bg supplement Author(s) 215. CC Attribution 3. License. Supplement of Quantifying environmental stress-induced emissions of algal isoprene and monoterpenes using laboratory measurements N. Meskhidze et al. Correspondence to: N. Meskhidze
2 Table S1. Purge and Trap Conditions CDS 8 Sample Purge Desorb Bake 4 ml/min for 35 min 25 C for 5 min 26 C for 5 min Temperatures Line 25 C, Valve 26 C, Wet trap 2 C GC Parameters Oven Temp Temperature Ramp Column 5 C for 2 minute hold From 5 C to 25 C at 6 C/min CP-PoraBOND PLOT Q Fused Silica Injector Split, 25 C Split ratio Carrier Gas 1:1 for.75 min, 1:1 for 2.25 min, 2:1 till end Ultra High Purity Helium MS Parameters Scan Time Ionization Mode Emission Current Mass Range Delay* 3 seconds/scan Electron Ionization (EI) 2 µamps 35-3 m/z 5 min GC/MS Transfer Line Temperature 18 C Ion Trap Temperature 18 C *Delay set to avoid "dead volume" signal in chromatograph
3 Table S2. production rates from phytoplankton monocultures as a function of time T. weissflogii 9 μmol m -2 s μmol m -2 s μmol m -2 s μmol m -2 s μmol m -2 s μmol m -2 s μmol m -2 s μmol m -2 s T. pseudonana 9 μmol m -2 s μmol m -2 s μmol m -2 s μmol m -2 s μmol m -2 s μmol m -2 s μmol m -2 s μmol m -2 s P. carterae 9 μmol m -2 s μmol m -2 s μmol m -2 s μmol m -2 s μmol m -2 s μmol m -2 s μmol m -2 s μmol m -2 s
4 9 μmol m -2 s μmol m -2 s μmol m -2 s μmol m -2 s μmol m -2 s μmol m -2 s μmol m -2 s μmol m -2 s P. minimum 9 μmol m -2 s μmol m -2 s μmol m -2 s μmol m -2 s μmol m -2 s μmol m -2 s μmol m -2 s μmol m -2 s μmol m -2 s μmol m -2 s μmol m -2 s μmol m -2 s μmol m -2 s μmol m -2 s μmol m -2 s μmol m -2 s
5 Table S3. production rates from phytoplankton monocultures as a function of time T. weissflogii x μmol m -2 s μmol m -2 s μmol m -2 s μmol m -2 s μmol m -2 s μmol m -2 s μmol m -2 s μmol m -2 s T. pseudonana x μmol m -2 s μmol m -2 s μmol m -2 s μmol m -2 s μmol m -2 s μmol m -2 s μmol m -2 s μmol m -2 s P. carterae x μmol m -2 s μmol m -2 s μmol m -2 s μmol m -2 s μmol m -2 s μmol m -2 s μmol m -2 s μmol m -2 s
6 x μmol m -2 s μmol m -2 s μmol m -2 s μmol m -2 s μmol m -2 s μmol m -2 s μmol m -2 s μmol m -2 s P. minimum x μmol m -2 s μmol m -2 s μmol m -2 s μmol m -2 s μmol m -2 s μmol m -2 s μmol m -2 s μmol m -2 s x μmol m -2 s μmol m -2 s μmol m -2 s μmol m -2 s μmol m -2 s μmol m -2 s μmol m -2 s μmol m -2 s
7 Figure S1. Sample chromatographs for 38 different BVOC screened. Each labeled spike corresponds to a listed compound on the right hand side.
8 rradiance flux (μg g -1 Chl-a h -1 ) Production rate calculated at respective time step and irradiance (mol cell -1 day -1 ) 25over the first 12 hour sampling cycle (stress) T. weiss logii 9 mol m -2 s mol m -2 s mol m -2 s -1 9 mol m -2 s -1 Time step from sampling time hours (units in hours) flux (μg g -1 Chl-a h -1 ) Produ respecd at (mol c rradiance hour s second 12 mated) T. pseudonana 9 mol m -2 s mol m -2 s mol m -2 s -1 9 mol m -2 s -1 flux (μg g -1 Chl-a h -1 ) P. carterae 9 mol m -2 s mol m -2 s mol m -2 s -1 9 mol m -2 s -1 m-2 s-1 m-2 s-1 m-2 s-1 2 s-1 flux (μg g -1 Chl-a h -1 ) mol m -2 s mol m -2 s mol m -2 s -1 9 mol m -2 s flux (μg g - 1 Chl-a h -1 ) P. minimum 9 mol m -2 s mol m -2 s mol m -2 s -1 9 mol m -2 s mol m -2 s mol m -2 s Irradianc mol m -2 s mol m -2 s flux (μg g -1 Chl-a h -1 ) Figure S2. Light stress production rates for isoprene (µg (g Chl-a) -1 h -1 ) as a function of time during the first ( to 12 hours) and the second (12 to 24 hours) light cycle. Note that there was a 12-hour dark period between the two five point sample sets and that the first samples in each set were collected 2 hours after lights on. Error bars denote the RSD Total value listed in Table 1.
9 flux (g cell -1 h -1 ) 1.4E E-17 1.E-17 Phytoplankton Species 8.E-18 Production rate calculated at respective time step and irradiance (mol cell -1 day -1 ) over the 6.E-18 first 12 hour sampling cycle (stress) 4.E-18 Time step from sampling time hours (units in hours) T. weiss. T. pseud. P. carter. P. minim. Prod irrad sam flux (g cell -1 h -1 ) 1.4E E-17 1.E-17 8.E-18 6.E-18 4.E-18 T. weiss. T. pseud. P. carter. P. minim. 2.E-18 2.E-18.E Irradiance μmol m -2 s -1.E Irradiance μmol m -2 s -1 Figure S3. The 12-hour averaged isoprene production rates (g cell -1 h -1 ) for the first (left column) and second (right column) light cycle as a function of irradiance. Error bars denote the RSD Total value listed in Table 1.
10 α-pinene flux (g cell -1 h -1 ) 4.5E-19 4.E E-19 3.E E-19 2.E E-19 1.E-19 5.E-2 T. weiss. T. pseud. P. carter. P. minim. α-pinene flux (g cell -1 h -1 ) 4.5E-19 4.E E-19 3.E E-19 2.E E-19 1.E-19 5.E-2 T. weiss. T. pseud. P. carter. P. minim..e Irradiance μmol m -2 s -1.E Irradiance μmol m -2 s -1 Figure S4. The 12-hour averaged α-pinene production rates (g cell -1 h -1 ) for the first (left column) and second (right column) light cycle as a function of irradiance. Error bars denote the RSD Total value listed in Table 1.
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