User s Guide for software version 2.1

Transcription

1 FluorEssence User s Guide v. 2.0 (6 Dec 2006) User s Guide for software version (All HORIBA Jobin Yvon companies were formerly known as Jobin Yvon) 1

2 Copyright 2006 by HORIBA Jobin Yvon Inc. All rights reserved. No part of this work may be reproduced, stored, in a retrieval system, or transmitted in any form by any means, including electronic or mechanical, photocopying and recording, without prior written permission from HO- RIBA Jobin Yvon Inc. Requests for permission should be requested in writing. Origin is a registered trademark of OriginLab Corporation. RealPlayer is a registered trademark of Real Networks, Inc. InstallShield is a registered trademark of InstallShield Software Corporation. Windows is a trademark of Microsoft Corporation. Information in this manual is subject to change without notice, and does not represent a commitment on the part of the vendor. December 2006 revision 2.0 Part Number

3 FluorEssence User s Guide v. 2.0 (6 Dec 2006) Table of Contents 0: Introduction... 4 About FluorEssence...4 Disclaimer...5 Symbols used in this manual...7 1: Installation... 8 Requirements...8 Installation instructions...9 2: Quick Guide to Running a Scan : Tips & Tricks...31 Calibration of your instrument...31 Using corrected signals...39 Projects and files...40 Saving and recalling a file : Shutting Down : Un-Installation instructions : Troubleshooting & Technical Support...53 Troubleshooting...53 On-line help...54 Video tutorials...55 If you have a technical problem...56 Contact information : Index

4 0: Introduction About FluorEssence FluorEssence is the easiest data-acquisition software ever created by Spex Fluorescence. All aspects of spectrofluorometer-control are available with only a few mouse-clicks or keystrokes, with a minimum of overlapping screens and windows. Data can be previewed while they are being recorded, and then immediately used with Origin presentation and graphical analysis. FluorEssence runs using Windows 2000 or XP Pro. 4 Note: Keep this and the other reference manuals near the system.

5 FluorEssence User s Guide v. 2.0 (6 Dec 2006) Disclaimer By setting-up or starting to use any HORIBA Jobin Yvon product, you are accepting the following terms: You are responsible for understanding the information contained in this document. You should not rely on this information as absolute or all-encompassing; there may be local issues (in your environment) not addressed in this document that you may need to address, and there may be issues or procedures discussed that may not apply to your situation. If you do not follow the instructions or procedures contained in this document, you are responsible for yourself and your actions and all resulting consequences. If you rely on the information contained in this document, you are responsible for: Adhering to safety procedures Following all precautions Referring to additional safety documentation, such as Material Safety Data Sheets (MSDS), when advised As a condition of purchase, you agree to use safe operating procedures in the use of all products supplied by HORIBA Jobin Yvon, including those specified in the MSDS provided with any chemicals and all warning and cautionary notices, and to use all safety devices and guards when operating equipment. You agree to indemnify and hold HORIBA Jobin Yvon harmless from any liability or obligation arising from your use or misuse of any such products, including, without limitation, to persons injured directly or indirectly in connection with your use or operation of the products. The foregoing indemnification shall in no event be deemed to have expanded HORIBA Jobin Yvon s liability for the products. HORIBA Jobin Yvon products are not intended for any general cosmetic, drug, food, or household application, but may be used for analytical measurements or research in these fields, or for forensic applications. A condition of HORIBA Jobin Yvon s acceptance of a purchase order is that only qualified individuals, trained and familiar with procedures suitable for the products ordered, will handle them. Training and maintenance procedures may be purchased from HORIBA Jobin Yvon at an additional cost. HORIBA Jobin Yvon cannot be held 5

6 responsible for actions your employer or contractor may take without proper training. Due to HORIBA Jobin Yvon s efforts to continuously improve our products, all specifications, dimensions, internal workings, and operating procedures are subject to change without notice. All specifications and measurements are approximate, based on a standard configuration; results may vary with the application and environment. Any software manufactured by HORIBA Jobin Yvon is also under constant development and subject to change without notice. Any warranties and remedies with respect to our products are limited to those provided in writing as to a particular product. In no event shall HORIBA Jobin Yvon be held liable for any special, incidental, indirect or consequential damages of any kind, or any damages whatsoever resulting from loss of use, loss of data, or loss of profits, arising out of or in connection with our products or the use or possession thereof. HORIBA Jobin Yvon is also in no event liable for damages on any theory of liability arising out of, or in connection with, the use or performance of our hardware or software, regardless of whether you have been advised of the possibility of damage. 6

7 FluorEssence User s Guide v. 2.0 (6 Dec 2006) Symbols used in this manual Certain symbols are used throughout the text for special conditions when operating the instruments: Note: General information is given concerning operation of the equipment. 7

8 1: Installation Requirements To successfully install FluorEssence, your host computer needs the following: Software Windows 2000 or Windows XP Pro Hardware Supports Windows 2000 or Windows XP Pro 128 MB RAM (256 MB recommended) 200 MB hard-disk space One available Ethernet Network Interface Card (NIC) connection (no hubs) One available USB port Video resolution of at least

9 FluorEssence User s Guide v. 2.0 (6 Dec 2006) Installation instructions 1 Insert the FluorEssence CD-ROM in the host computer s CD-ROM drive. 2 If Autorun is not operating, continue here: a Open the My Computer icon. b The My Computer window opens: c Click on the CD-ROM drive to open the FluorEssence CD-ROM: d e Click the Setup.exe icon. Continue with step 3 below. 9

10 3 If Autorun is operating, continue here, to install FluorEssence software: The InstallShield Wizard starts. a Click Next >. The License Agreement appears. 10 b Click I accept the terms of the license agreement radio button, then Yes. The Customer Information area appears.

11 FluorEssence User s Guide v. 2.0 (6 Dec 2006) c Enter your User Name and Company Name. The Next > button activates. d Click Next >. The Choose Destination Location area appears. e Choose the location (in the Destination Folder) where FluorEssence is to be installed. 11

12 Most people prefer the default location, shown in the Destination Folder area. f Click Next >. The Start Copying Files area appears. Review the Current Settings in the list. g Click Next >. The computer starts copying the files from the CD-ROM to the hard-drive, and the Setup Status window appears: When copying is complete, the Unplug Software Key window opens: 12

13 FluorEssence User s Guide v. 2.0 (6 Dec 2006) h If the USB key is installed in a USB port, remove it now. i Click Next >. The InstallShield Wizard Complete window opens: j k Choose the Yes radio button to restart. Click Finish. 13

14 If you clicked Yes, the host computer reboots. In case the computer does not automatically reboot, reboot manually. Note: If you do not restart, FluorEssence will not operate correctly until the next restart. 4 Remove the FluorEssence CD-ROM from the host computer. The InstallShield Wizard starts again. The System Configuration Wizard opens. 5 Choose a hardware configuration to run. 14 a Choose one possible hardware configuration that your system can run correctly. You may choose a radio button for The exact hardware setup that HORIBA Jobin Yvon built for you. A basic hardware configuration, for example, a typical FluoroMax -3. Your own hardware setup in which every component can be tailored. b Click Next >.

15 FluorEssence User s Guide v. 2.0 (6 Dec 2006) If you chose Load Factory Configuration: The Select a File window opens. a Browse through the folders, and select the desired.jyc file. The InstallShield Wizard Complete window opens. b Continue with step 6 on page 21. If you chose Standard Instrument Configuration: The System Selection Page opens. a From the drop-down menus, choose the System Type and Model. b Click Next >. The Configuration Name page opens. 15

16 c Use the default name, or enter your own in the field. d Click Next >. The Instrument Configuration page opens. 16

17 FluorEssence User s Guide v. 2.0 (6 Dec 2006) e Choose the appropriate settings, leave the defaults, or adjust as required. Click the checkboxes in the Available Accessories area to activate all desired and available accessories. Note: If you select a Sample Changer as an accessory, a window appears asking which Sample Changer: 2-position or 4-position. If you select a Temperature Controller as an accessory, a window appears asking for its details: be sure to choose the correct Manufacturer. f Click Next >. The Summary page opens. g h Examine the Summary page to be sure that your configuration is correct. To change the entries, click < Back. Click Finish. The InstallShield Wizard Complete window opens. i Continue with step 6 on page

18 If you chose Detailed Component Configuration: Note: Detailed Component Configuration should be performed only by advanced users. HORIBA Jobin Yvon recommends the Standard Instrument Configuration or Load Factory Configuration instead. The Device type area opens. a Choose a component of your instrument to add from the menu. In this case, a monochromator was selected. b Click Next >. The Module type area opens. 18

19 FluorEssence User s Guide v. 2.0 (6 Dec 2006) c Choose the particular model of the component from the menu. d Click Next >. The General information area opens. 19

20 e Enter a name or description for the new component, or use the default provided. f Click Next >. The Communications Parameters area opens. g Choose the parameters, or accept the default values. h Click Next >. The Accessory information area opens. 20

21 FluorEssence User s Guide v. 2.0 (6 Dec 2006) i Choose the parameters, or accept the default values. j Click Next >. The Summary area appears. k Review for correctness, and click Apply. 21

22 The Device Type area reappears. l m Continue to add new components until the system configuration is complete. When complete, choose No more device. Exit. The New Configuration window appears. n o Enter a brief name and a long name for the hardware configuration in the fields provided. Click OK. The InstallShield Wizard Complete window opens. 22

23 FluorEssence User s Guide v. 2.0 (6 Dec 2006) 6 To launch FluorEssence immediately, check the Launch FluorEssence checkbox. 7 Click Finish. The Information window appears. 8 Plug in the USB software key in a free USB port on the host computer. 9 Click OK. The Information window vanishes, and, if you checked the box marked Launch, FluorEssence starts. 23

24 2: Quick Guide to Running a Scan 1 Turn on the host computer, and all instruments and accessories, as explained in their respective instruction manuals. 2 Click on the FluorEssence short-cut to start Fluor- Essence. The FluorEssence window opens. There are nine special buttons for running experiments in FluorEssence : 24

25 FluorEssence User s Guide v. 2.0 (6 Dec 2006) Experiment Menu Previous Experiment Auto Run Previous Experiment Choose an overall type of experiment to run, such as Phosphorescence, general Spectra (e.g., emission or excitation), or Single Point. Modify slightly a previously set-up experiment, and run it. Run a previously set-up experiment without modification. Run JY Batch Experiments Real Time Control Make Overlay File 3D Scan to 3D Profile Create/Use Calibration Curve from CWA Data Launch DataStation button Run an automated series of experiments, including adjustable repeats and delays between experiments. Open the Real Time Control window directly, to adjust experimental parameters in real time. With an existing graph selected, create an.spc file for use as an overlay file. The existing graph should contain a single spectrum. Extract excitation and emission profiles from an excitation-emission matrix. The active file must be such a data matrix. From Single Point experiments, create and use a calibration curve for analytical measurements. Closes FluorEssence software, and then starts DataStation software. Note: This operates only with TCSPC accessories. From many of these buttons, upon initial start-up of the software, you can choose a hardware configuration and experiment type. After a hardware configuration is loaded, each button has its own separate function. 25

26 The Collect menu near the top of the main window also has these functions, plus another important command, System UI Reset. In case the six special buttons are grayed out, choose the System UI Reset command. See Chapter 5: Technical Support for more details. 3 Click the Experiment Menu button. The Select Hardware Configuration window opens. Note: This window does not appear if you have only one hardware configuration installed. Skip to page To force the appearance of the Select Hardware Configuration window:

27 FluorEssence User s Guide v. 2.0 (6 Dec 2006) a Or b Immediately upon opening FluorEssence, press F8 and simultaneously click the Experiment Menu button,. At any time within FluorEssence, press F8 while choosing the Collect Menu / Advanced Setup / System ReInitialization. 4 Choose a system configuration from the list, then click OK. FluorEssence loads the chosen system configuration. The Fluorescence Main Experiment Menu appears: 5 Click an available scan type, say, Spectra. 27

28 The Experiment Type window appears. 6 Choose an Experiment Type from the menu, say, Emission. The parameters required for an emission scan are loaded into the Experiment Setup window, which appears. For an emission scan, the default parameters are a water-raman scan. 7 Click Next >>. The Experiment Setup window opens. 8 Click the Experiment File field, and enter a new file name or select a previously saved file. 28

29 FluorEssence User s Guide v. 2.0 (6 Dec 2006) 9 Verify that the experimental parameters are correct. Be certain to check all parameters under all icons in the lefthand column. 10 Insert the sample into the sample compartment, and close the cover of the sample compartment. 11 Click Run. The collected spectrum is displayed on the Intermediate Display screen: Note: If the scan is extremely fast, the Intermediate Display may be only incompletely or rapidly displayed before the Origin window appears. You can watch the incoming data in real time, along with how the positions of accessories vary. The scan may be paused, continued, or aborted. After all data are recorded, the Intermediate Display vanishes. For a new project, the Project name window appears: 29

30 12 Enter a name for the entire project, or browse for an existing project name with the Browse button, then click OK. All data are moved to Origin s graph window: 13 Do post-processing as needed, using the Analysis menu. 30

31 FluorEssence User s Guide v. 2.0 (6 Dec 2006) 3: Tips & Tricks Calibration of your instrument Excitation calibration Monochromator parameters for the xenon-lamp scan: Monochromator (1200 grooves/mm) Initial wavelength Final wavelength Increment Excitation 200 nm 600 nm 1 nm 1 nm Emission 350 nm nm Detector parameters for the xenon-lamp scan: Detector (Signal) Integration time Units Signal (S1) 100 ms CPS Slits (bandpass) Reference (R1) 100 ms ma 1 Close the sample compartment s lid. 2 Start FluorEssence. 3 In the main FluorEssence window, choose the Experiment Menu button. The Fluorescence Main Experiment Menu appears. 4 Choose Spectra. The Experiment Type window appears. 31

32 5 Choose Excitation. The xenon-lamp scan automatically loads. 6 Click Run. The Intermediate Display opens. The xenon-lamp scan runs: 32

33 FluorEssence User s Guide v. 2.0 (6 Dec 2006) Above is an uncalibrated FluoroMax lamp-scan. The main peak ought to be at 467 nm, but here appears near 480 nm. 7 Calibrate the excitation monochromator, if required. a b Expand the plot by clicking the Expand button. Click the cursor button to start the cursor function. c d Click on the graph near the peak, to place the cursor on the graph. Using the left and right arrows on the keyboard, move the cursor to the top of the peak. e Read the x- value of the plot: this is the wavelength of the peak: Note: Your lamp scan may appear different, depending on the instrument and its configuration. 33

34 Cursor Wavelength in nm This example shows the peak at 477 nm, which is 10 nm too high. Therefore we must calibrate the monochromator. f Click the Previous Experiment button. The Experiment window appears: 34

35 FluorEssence User s Guide v. 2.0 (6 Dec 2006) g Click the RTC button on the lower right. The Real Time Control window opens. h Click the Monos icon to view the monochromators index card, then click the excitation monochromator tab. i Enter the current, observed position of the peak in the Position Control (here, 477 nm). j Click Calibrate Excitation 1. The Calibrate window opens: 35

36 k l m n In Peak of Interest, enter the actual or expected position of the peak (it ought to be 467 nm). Click OK. At the bottom right of the Real Time Control window, click Cancel. In the Experiment window, click Run to confirm the correct peak position. A correct scan is shown below (peak is at 467 nm). Cursor Wavelength in nm 36

37 FluorEssence User s Guide v. 2.0 (6 Dec 2006) Emission Calibration Monochromator parameters for the water-raman scan: Monochromator (1200 grooves/mm) Initial wavelength Final wavelength Increment Excitation 350 nm nm Emission 365 nm 450 nm 1 nm 5 nm Detector parameters for the water-raman scan: Detector (Signal) Integration time Units Signal (S1) 100 ms CPS Slits (bandpass) Reference (R1) 100 ms ma 1 Insert a cuvette with HPLC-grade, triple-distilled water in the sample compartment. 2 Close the sample compartment s lid. 3 Start FluorEssence. 4 In the main FluorEssence window, choose the Experiment Menu button. The Fluorescence Main Experiment Menu appears. 5 Choose Spectra. The Experiment Type window appears: 37

38 6 Choose Emission. The water- Raman experiment automatically loads Click Run. The Intermediate Display opens. The water-raman scan runs. 8 If the water-raman scan is not at 397 nm, calibrate the emission monochromator as shown on pages

39 FluorEssence User s Guide v. 2.0 (6 Dec 2006) Using corrected signals Introduction Subtracting blanks, removing dark noise, and correcting for inhomogeneities in the instrument or detector response give more accurate spectra. Take special precautions to incorporate these functions properly into a FluorEssence experiment. Method Any corrected signal (with a subscript c ) or algebraic use of corrected signals must explicitly include all desired corrected signals in the Formulas list. Corrected signals include: Dark Offset Blank Subtraction Correction-factor file Example Note: Be sure all desired corrections are activated in their respective checkboxes. Note how the corrected signal, S1c, and corrected reference, R1c, along with their ratio, S1c/R1c, all must be included in the Formulas list in the Signal Algebra area. 39

40 Projects and files What is a project? A project is a collection of data that contains: Graphs (visual diagrams of the data) Worksheets (tables of data) Notes (comments about the data) Project Graphs Worksheets Notes Layers Datasets Axes Backgrounds Graphs themselves may contain multiple kinds of information, including separate layers describing the data, the axes, the background colors, etc. Concerning worksheets, a dataset must contain at least two columns, corresponding to x-y data pairs. Multiple y columns may correspond to a single x column. Note: For greater detail about projects, graphs, layers, and how to merge, combine, and separate them, see the Origin on-line help files. 40

41 FluorEssence User s Guide v. 2.0 (6 Dec 2006) Combining two plots (datasets) into one graph In its upper left corner, an open graph has a small box with a number in it. 1 Right-click on the numbered box in the upper left corner of the graph. A dropdown menu appears. 2 Choose Layer Contents... The Layer Number window opens. In the Available Data column, a list of x-y data available to plot is shown. 41

42 3 Click on the x-y dataset you wish to add to the plot. Note: For datasets with multiple y columns with one x column, the first y column plus the x column is listed as ***_a, and the second y column plus the x column is listed as *** b, etc. 4 Choose => to add this dataset to the plot. The dataset appears in the Layer Contents column. 5 Adjust its order (top to bottom) with the and buttons. 6 Click OK to cause the dataset to appear in the graph. The Layer Number window closes. 42

43 FluorEssence User s Guide v. 2.0 (6 Dec 2006) Merging two or more graph windows This puts all the open layers on one single page. 1 Close all graph windows you don t want to merge. In the Project Explorer at the bottom of the main Fluoresence window, double-click on the names of the undesired open graphs. These graphs close. 2 Click the Merge button. All the windows are combined onto one sheet of paper. This is called a graph. To preserve the old graphs while creating a new, overlaid version, answer Yes to the question, Do you wish to keep the old graphs? 3 A window appears asking you for numbers of rows and columns. To exactly overlay the graphs, choose 1 row and 1 column. Note: Each layer has its own number in the upper left corner. 43

44 Splitting two graphs by extraction This extracts each plot to a separate layer in the graph. 1 Click on the desired plot to activate it. 2 In the toolbar, choose the Extract to Layers button. Note: Other buttons available using the Customize Toolbar command are the button for splitting each layer into a separate graph window, and the button for merging all open graph windows into one graph. See the Origin on-line help for more information. 44

45 FluorEssence User s Guide v. 2.0 (6 Dec 2006) Saving and recalling a file To save an experiment, when in a new, untitled experiment Note: To determine if you are in an untitled, new experiment, examine the path shown at the top of the main FluorEssence window. It should show the word UNTI- TLED at the end of the path. 1 Run the experiment. When the experiment is complete, the Intermediate Display disappears. The Project Name window appears. 2 Enter a new name for the project, or browse for an existing one. Note: If you are using an existing project name, the software will allow you to overwrite existing data, or append the new data to the project. 3 Click OK. The path of the project appears at the top of the main FluorEssence window. The data are now saved. To save data into a new project when another project is already open 1 Run the experiment. 2 Choose File. The File menu opens: 45

46 3 Choose New. The New window opens: 4 Choose Project from the list of objects to create. Note: Only a Graph or a Matrix lets you pick a new Path and Name. 5 Click OK. The data are now in an untitled project. You must next create the name for the file. 6 Choose File again. The File menu opens. 7 Choose Save Project As... The Save As window appears: 46

47 FluorEssence User s Guide v. 2.0 (6 Dec 2006) 8 In the File name field, enter a name. In the Save as type field, choose Project (*.opj) from the list. 9 Click Save. Now the new project has a name. To recall and open an existing project 1 Click File. The File menu opens. 2 Choose Open... The Open window appears. 3 Browse for the desired project. 47

48 4: Shutting Down FluorEssence 1 Save experiment files (and data files, if created). 2 In the Experiment Setup window, click the Close button. 3 Close the FluorEssence window, using either the Close button, or, in the File dropdown menu, Exit. 48

49 FluorEssence User s Guide v. 2.0 (6 Dec 2006) 5: Un-Installation Instructions 1 Close FluorEssence. 2 Click the Start button to open the Start menu. 3 There are two ways to continue: a b Choose Set Program Access and Defaults. Choose Control Panel. The Control Panel opens. 49

50 Click Add/Remove Programs. 4 In both cases, continue here. The Add or Remove Programs window opens. 5 Click the Change or Remove Programs icon. A list of currently installed programs on the host computer appears: 50

51 FluorEssence User s Guide v. 2.0 (6 Dec 2006) 6 Click FluorEssence, which becomes active: 7 Click Change/Remove. 8 Follow the instructions to remove Fluor- Essence. 9 Click on SuperProNet Combo Installer, which becomes active: 51

52 10 Click Remove. 11 Follow the instructions to remove SuperProNet Combo Installer. 12 You may need to reboot the host computer. FluorEssence is removed from the host computer. 13 Remove the USB key from the USB port. 52

53 FluorEssence User s Guide v. 2.0 (6 Dec 2006) 6: Troubleshooting & Technical Support Troubleshooting If the nine special buttons are gray, 1 Choose Collect. A drop-down menu appears. 2 Choose System UI Reset. The four buttons should become active again. 53

54 On-line help files Access from the main FluorEssence window 1 Choose the Help menu. A drop-down menu appears. 2 Choose Data Acquisition. The on-line help files appear. Resize the window to your liking. Access from the Experiment Setup or Real Time Control window 1 Click Help or F1. Context-sensitive on-line help files appear. Resize the window to your liking. 54

55 FluorEssence User s Guide v. 2.0 (6 Dec 2006) Video tutorials For some common procedures, video tutorials are available to guide you. The videos are.avi files, which can be played by software such as RealPlayer, Windows Media Player, etc. Access to video tutorials Note: The video tutorials are narrated in English. A sound-card and speakers are required on your host PC. 1 Click the Windows Start button. The Start menu appears. 2 Choose All Programs. 3 Choose the Jobin Yvon group. 4 Choose the Video Tutorials subgroup. 5 Click on the desired tutorial. The tutorial opens in your chosen video-playing software. 55

56 If you have a technical problem, 1 Please consult the FluorEssence help files and this manual, as well as all other manuals supplied with the system. If you are unable to solve the problem, 2 Note the problem and any accompanying error messages. 3 Determine FluorEssence s version number. a Choose the Help menu. b Choose About FluorEssence... The About FluorEssence window opens. Near the bottom are the FluorEssence and Origin version numbers. 56 c Click the View System Info button.

57 FluorEssence User s Guide v. 2.0 (6 Dec 2006) d The Installed Components window appears, displaying all the software required for FluorEssence. e Record the information by clicking the: Save To File... button, which saves the information to a file; Zip Info button, which compresses the information while saving it; Print Info button, which prints out the software information. f Click OK to close the Installed Components window. g Click OK to close the About FluorEssence window. 4 Write down the software s version numbers, along with the purchase dates, model numbers, system configuration, and serial numbers of the instrument and its accessories. 5 Please contact a Spex Fluorescence Service Department listed below. 57

58 Be prepared to describe the malfunction and the attempts, if any, to correct it. Note any error messages observed, and have any relevant spectra (sample, polarization ratio, xenonlamp scan, water Raman scan, etc.) and system information ready for us to assist you. Contact information In North America: Telephone Fax World-Wide Web Technical support hours are 8:00 A.M. 5:00 P.M., Monday though Friday, Eastern Standard/Daylight Time. In France: Telephone +33 (0) Fax +33 (0) World-Wide Web Worldwide: China +86 (0) Germany +49 (0) Italy +39 (0) Japan +81 (0) UK +44 (0)

59 FluorEssence User s Guide v. 2.0 (6 Dec 2006) 7: Index Key to the entries: Times Roman subject or keyword font Arial font command, menu choice, or dataentry field Arial Condensed Bold font Courier New font < dialog box file name or expression < Back = => button button D Scan to 3D Profile button A Abort button About FluorEssence window... 56, 57 About FluorEssence accessories...24, 29, 57 Accessory information area Add or Remove Programs window. 50 Add/Remove Programs Advanced Setup All Programs Analysis menu Apply Auto Run Previous Experiment button Autorun... 9, 10.avi files Available Accessories area Available Data column axes B background color batch experiments blank subtraction Browse button C Calibrate Excitation Calibrate window calibration... 33, 38 curve CD-ROM...9, 12, 14 Change or Remove Programs icon. 50 Change/Remove Choose Destination Location area Close button... 26, 48 Collect menu... 27, 53 columns Communications Parameters area.. 20 Company Name Configuration Name page contact information Continue button Control Panel

60 corrected signals correction factor files CPS... 31, 37 Create/Use Calibration Curve from CWA Data button Current Settings cursor button Customer Information area cuvette D dark noise offset Data Acquisition dataset... 40, 41, 42 DataStation software Destination Folder... 11, 12 area Detailed Component Configuration 18 Device type area... 18, 22 disclaimer... 5 E Emission... 28, 38 emission calibration monochromator scan error messages... 56, 58 Ethernet Network Interface Card (NIC) connection... 8 Excitation excitation calibration monochromator... 33, 35 -emission matrix Exit Expand button Experiment File field Experiment Menu button.25, 26, 27, 31, 37 Experiment Setup window... 28, 48, 54 experiment type Experiment Type Experiment Type window... 31, Experiment window... 34, 36 Extract to Layers button F F1 button F8 button File...45, 46, 47 menu...45, 46, 47 name final wavelength... 31, 37 Finish...13, 17, 23 Fluorescence Main Experiment Menu 27, 31, 37 FluorEssence short-cut FluorEssence window FluoroMax... 14, 33 Formulas list G General information area graph...30, 40, 41, 42, 43, 44 grayed out buttons H hard-disk space... 8 hardware configuration...14, 22, 25 Help menu... 54, 56 help files host computer...8, 9, 14, 23, 24, 50, 52 I increment... 31, 37 Information window initial wavelength... 31, 37 installation requirements... 8 Installed Components window InstallShield Wizard Complete window...13, 15, 17, 22 InstallShield Wizard... 10, 14

61 FluorEssence User s Guide v. 2.0 (6 Dec 2006) Instrument Configuration page integration time... 31, 37 Intermediate Display...29, 32, 38, 45 J Jobin Yvon group jyc files L Launch DataStation button Launch FluorEssence Layer Contents column Layer Number window... 41, 42 layers... 40, 43 License Agreement Load Factory Configuration M ma... 31, 37 Make Overlay File button Merge button merging two or more graph windows.. 43 Model Module type area Monos icon My Computer icon... 9 My Computer window... 9 N New New Configuration window New window Next >.10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 28 No more device. Exit notes O OK button...22, 27, 30, 36, 42, 45, 46, 57 on-line help Open window Open Origin... 4, 30 P Pause button Peak of Interest Position Control post-processing Previous Experiment button... 25, 34 Print Info button project...29, 30, 40, 45, 47 Project (*.opj) Explorer Project name window... 29, 45 R R , 37 R1c RAM... 8 Raman...See water Raman scan Real Time Control button Real Time Control window.25, 35, 36, 54 RealPlayer reboot recall... 45, 47 reference... 31, 37 Remove... 14, 52 rows RTC button Run button...29, 32, 36, 38 Run JY Batch Experiments button.. 25 S S , 37 S1c S1c/R1c

62 sample compartment... 29, 31, 37 Save as type Project As To File... button Save As window saving Select a File window Select Hardware Configuration window serial number Service Department Set Program Access and Defaults.. 49 Setup Status window Setup.exe icon... 9 signal... 31, 37 Signal Algebra area Single Point slits... 31, 37.SPC files Spectra...25, 27, 31, 37 splitting two graphs Standard Instrument Configuration. 15 Start button... 49, 55 Copying Files area menu... 49, 55 Summary area Summary page SuperProNet Combo Installer... 51, 52 symbols... 7 system configuration... 27, 57 System Configuration Wizard System ReInitialization System Selection Page System Type System UI Reset... 26, 53 U units... 31, 37 Unplug Software Key window USB key...13, 23, 52 USB port...8, 13, 23, 52 User Name V version number... 56, 57 video resolution... 8 video tutorials Video Tutorials subgroup View System Info button W water-raman scan...28, 37, 38, 58 Windows Media Player Windows... 4, 8 worksheet X x column xenon-lamp scan...31, 32, 33, 58 x-y data pairs Y y column Yes button...10, 13, 14, 43 Z Zip Info button

63 FluorEssence User s Guide v. 2.0 (6 Dec 2006) 63

64 USA: HORIBA Jobin Yvon Inc., 3880 Park Avenue, Edison, NJ , Toll-Free: jobinyvon Tel: , Fax: , France: HORIBA Jobin Yvon S.A.S., 16-18, rue du Canal, Longjumeau Cedex, Tel: +33 (0) , Fax: +33 (0) , Japan: HORIBA Ltd., JY Optical Sales Dept, Higashi-Kanda, Daiji Building, Higashi-Kanda Chiyoda-ku, Tokyo , Tel: +81 (0) , Germany: +49 (0) Italy: UK: +44 (0) China: +86 (0)