Alternative Filters (all non-standard)

Transcription

1 Fluorochrome Excitation (nm) Emission (nm) Best Filter (available) LP Alternative Filters (all non-standard) Notes 7-AAD 488; /50;670/30 685; /30 BP with 635LP* NOTE: very high spectral spillover into 670/30 BP from 561nm Yellow laser Alexa Fluor /50 - Alexa Fluor /50 - Alexa Fluor / Alexa Fluor / Alexa Fluor / Alexa Fluor / Alexa Fluor / /40 BP with 505LP Alexa Fluor /15-575/25 BP with 550LP Alternative filter set should only be used if using 488nm Blue laser for excitation Alexa Fluor /15-575/25 BP with 550LP Alternative filter set should only be used if using 488nm Blue laser for excitation Alexa Fluor / Alexa Fluor / Alexa Fluor / Alexa Fluor / /30 Alexa Fluor /14-670/30 Alexa Fluor /50* - This filter combo is NON-standard for 640nm Red laser Alexa Fluor / Alexa Fluor / Alexa Fluor / Currently unable to excite this dye with available lasers Alexa Fluor / Currently unable to excite this dye with available lasers AmCyan / APC /14-670/30 APC-Alexa / APC-Alexa / APC-Alexa / APC-Cy / APC-Cy / APC-eFluor / APC-Fire / APC-H / APC-R / APC-Vio / Azami Green / BB / BB Not released NR BB Not released NR BB Not released NR BB Not released NR BFP /50 - BUV /28 - BUV /50* 505 This filter combo is NON-standard for 355nm UV laser BUV /15* This filter combo is NON-standard for 355nm UV laser Prepared by Katherine Pilkington 08/05/2017 Page 1

2 BUV615P Not released BUV /20* 630 This filter combo is NON-standard for 355nm UV laser BUV / BUV /60* 750 This filter combo is NON-standard for 405nm Violet laser BV /50 - BV /40* This filter combo is NON-standard for 405nm Violet laser BV / BV Not released BV / BV / BV / BV750P Not released BV / BYG Not released Calcein Blue-AM /50 - Calcein Violet-AM /50 - Calcein-AM / Cascade Blue /50 - Cascade Yellow / Cell Proliferation Dye efluor /50 - Cell Proliferation Dye efluor /14-670/30 CellTrace BODIPY /20* 600* This filter combo is NON-standard for 488nm Blue laser CellTrace Far Red /14-670/30 CellTrace Oregon Green / CellTrace Violet /50 - CellTracker Blue 353; / /50 - CMAC/CMF 2 HC CellTracker Deep Red /14 - CellTracker Green / CellTracker Orange /15-585/42 WARNING: use this wider BP with care: spectral overlap with other dyes in 610/20BP CellTracker Red / CellTracker Violet / CellVue Burgundy / CellVue Claret /14 - CellVue Jade / CellVue Lavender /50 - CellVue Maroon /14-670/30 CellVue NIR / Currently unable to excite this dye with available lasers CellVue NIR / Currently unable to excite this dye with available lasers CellVue Plum /14 - Cerulean /50 - CFP /50 - CFSE / Chromeo / excellent dye for STED microscopy Chromeo /20* 600* This filter combo is NON-standard for 488nm Blue laser, excellent dye for STED microscopy Chromeo / excellent dye for STED microscopy Chromeo /42* - 586/15 This filter combo is NON-standard for 561nm Yellow/Green laser, excellent dye for STED microscopy Chrome /14 660/20 excellent dye for STED microscopy Prepared by Katherine Pilkington 08/05/2017 Page 2

3 Chromomycin A /50 we do not have the optimal laser for exciting this fluorochrome is wishing to analyse/sort chromosomes Cy / Cy /15-575/25 BP with 550LP Alternative filter set only to be used if using 488nm Blue laser for excitation, filter set is NON-standard for 488nm Blue laser Cy /15 - Cy /14-670/30 Cy / CyChrome / /30 BP with 635LP CytoPhase Violet /50 - DAPI /50 - DiI / /40 BP with 505LP DiO / DRAQ , /14-670/30 DRAQ7 600; /35-670/30 BP or 675/50 BP WARNING: multilaser excitation and 488nm lasers will also excite DRAQ7 DsRed /15-585/42 WARNING: use this wider BP with care: spectral overlap with other dyes in 610/20BP dtomato /15-585/42 WARNING: use this wider BP with care: spectral overlap with other dyes in 610/20BP DyLight /50 - DyLight /50 - DyLight / DyLight /15-585/42 WARNING: use this wider BP with care: spectral overlap with other dyes in 610/20BP DyLight / DyLight /14-670/30 DyLight /14 - DyLight / DyLight / Currently unable to excite this dye with available lasers DyLight / Currently unable to excite this dye with available lasers ECD /20* 600* This filter combo is NON-standard for 488nm Blue laser eflour 565 NC / /40 BP with 505LP efluor 490 NC / efluor 525 NC / efluor 585 NC /15-585/42 WARNING: use this wider BP with care: spectral overlap with other dyes in 610/20BP efluor 605 NC / efluor 625 NC / efluor 650 NC /14 - efluor /14-670/30 efluor 700 NC / Emerald / FITC / Fluo / FluoroGold 330, , /50;585/42* - 585/42 BP filter is NON-standard. NOTE: will spill into 450/50 BP and 586/14 BP from 405nm Violet laser FluorX / FP - - Fura * /30;525/ Variable excitation based on concentration of free Ca /30 with UV, 525/50 with 405nm FVD efluor /50 - FVD efluor 455UV /50 - FVD efluor / FVD efluor / FVD efluor /14-670/30 Prepared by Katherine Pilkington 08/05/2017 Page 3

4 FVD efluor / FVS /50 FVS / FVS / FVS /15-585/42 FVS / FVS /14-660/20 FVS FVS GFP / Helix NP Green /20* 600 This filter combo is NON-standard for 488nm Blue laser Helix NP NIR /20* - This filter combo is NON-standard for 640nm Red laser Hoechst Hoechst /50 - Hoechst /50 - Hoechst Blue /50 - Hoechst Red /30* 510* This filter combo is NON-standard, but is best for Side Population analysis Indo-1 (Blue) /50 - Indo-1 (Violet) /50 - Krome Orange / Live/Dead Aqua / Live/Dead Blue /50 - Live/Dead Far Red /14-670/30 Live/Dead Green / Live/Dead Near-IR / Currently unable to excite this dye with available lasers Live/Dead Red / Live/Dead Violet /50 - Live/Dead Yellow / /20 BP with 595LP Lucifer Yellow / Marina Blue /50 - mbanana / /40 BP with 505LP mcherry / mhoneydew / MitoTracker Deep Red /14-670/30 MitoTracker Green / MitoTracker Orange /15-585/42 WARNING: use this wider BP with care: spectral overlap with other dyes in 610/20BP MitoTracker Red /15 - mkate / mneptune / morange / /40 BP with 505LP mplum / mraspberry / mrfp / mstrawberry /15 - mtangerine /15-585/42 WARNING: use this wider BP with care: spectral overlap with other dyes in 610/20BP Oregon Green / Pacific Blue /50 - Prepared by Katherine Pilkington 08/05/2017 Page 4

5 Pacific Orange / PE 496; /15-585/42 WARNING: use this wider BP with care: spectral overlap with other dyes in 610/20BP PE-Alexa ; /20* 600* PE-Alexa ; /20* 600* If using 488nm Blue laser this filter combo is NON-standard; is standard for 561nm Yellow laser PE-Alexa ; / PE-CF ; /20* 600* If using 488nm Blue laser this filter combo is NON-standard; is standard for 561nm Yellow laser PE-Cy5 496; /30* 635* If using 488nm Blue laser this filter combo is NON-standard; is standard for 561nm Yellow laser PE-Cy ; / /35 BP with 670LP PE-Cy ; / /35 BP with 670LP PE-Cy7 496; /60* 750* If using 488nm Blue laser this filter combo is NON-standard; is standard for 561nm Yellow laser PE-Dazzle / PE-mCherry 496; /20* 600* If using 488nm Blue laser this filter combo is NON-standard; is standard for 561nm Yellow laser PerCP / /30 BP with 635LP PerCP-Cy / /35 BP with 670LP PerCP-eFluor / PerCP-Vio / PE-Texas Red 496/ /20* 600* If using 488nm Blue laser this filter combo is NON-standard; is standard for 561nm Yellow laser PE-Vio / /60* 750* If using 488nm Blue laser this filter combo is NON-standard; is standard for 561nm Yellow laser phrodo /15-585/42 WARNING: use this wider BP with care: spectral overlap with other dyes in 610/20BP PI /20* 600* If using 488nm Blue laser, this filter combo is NON-standard, if using 561nm Yellow laser this combo is standard POPO /15 - Pyronin Y /40* 545* RNA. This filter combo is NON-standard for 488nm Blue laser Qdot Qdot ; / Qdot ; /30;525/ /30 BP if using 355nm UV laser; 525/50 if using 405nm Violet laser Qdot ; /30;525/ /30 BP if using 355nm UV laser; 525/50 if using 405nm Violet laser Qdot ; /42*;586/ /42 BP if using 355nm laser, 586/15 if using 405nm laser. UV filter set is NON-standard Qdot ; /20* 570/ /20 BP with 570LP is NON-standard on UV laser; with 600LP is standard on 405nm laser Qdot ; /20* 630* If using 355nm UV laser this filter combo is NON-standard; is standard for 405nm Violet laser Qdot ; /50* 690* If using 355nm UV laser this filter combo is NON-standard; is standard for 405nm Violet laser Qdot ; /50* 690* If using 355nm UV laser this filter combo is NON-standard; is standard for 405nm Violet laser Qdot ; / RFP /15-585/42 WARNING: use this wider BP with care: spectral overlap with other dyes in 610/20BP RFP dtomato /15-575/25 BP with 550LP Alternative filter set only to be used if using 488nm Blue laser for excitation RFP tdtomato /15-585/42 WARNING: use this wider BP with care: spectral overlap with other dyes in 610/20BP Sapphire / SNARF 548/ / /15;610/20 ph 6/9; emission wavelength will depend on ph SYBR Green /30 DNA SYPRO Red / SYTOX Blue /50 - DNA SYTOX Green / DNA SYTOX Orange /15-575/25 BP with 550LP DNA. Alternative filter set only to be used if using 488nm Blue laser for excitation Tag-It Violet /50 - tdtomato /15-585/42 WARNING: use this wider BP with care: spectral overlap with other dyes in 610/20BP Texas Red / TO-PRO / TO-PRO /14-670/30 Prepared by Katherine Pilkington 08/05/2017 Page 5

6 TOTO / TOTO /14-670/30 TRITC /15-575/25 BP with 550LP Alternative filter set only to be used if using 488nm Blue laser for excitation UV UV V /50 - V / Venus / VioBlue /50 - VioBright FITC / VioGreen / Violet Violet Vybrant DyeCycle Green / Vybrant DyeCycle Orange 488; / /40 BP with 505LP Vybrant DyeCycle Ruby 488; /50;670/14 685; - Vybrant DyeCycle Violet 355; /50 - X-Rhodamine /15-585/42 WARNING: use this wider BP with care: spectral overlap with other dyes in 610/20BP YFP / Zombie Aqua / Zombie Green / Zombie NIR / Zombie Red / Zombie UV /50 - Zombie Violet /50 - Zombie Yellow / /20 BP with 595LP Prepared by Katherine Pilkington 08/05/2017 Page 6