R e p o r t E d i t o r M a n u a l

Transcription

1 R e p o r t E d i t o r 2. 1 M a n u a l

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3 Report Editor - Contents Page 1-1 Contents Page 1. Introduction Description of the Menu Procedures File Edit Format Tools View Window Help Toolbar Objects How to Use the Report Editor Creating a template (.RPF) Creating a template for the representation of multiple chromatograms Examples Index SCPA Software für Chromatographie und Prozess-Analytik GmbH Am Weidufer 32 D Weyhe Tel Fax info@scpa.de, Internet: Edition January 2004 for the Report Editor Version 2.1

4 Page Introduction - Report Editor 1. Introduction The Report Editor allows report print templates (.RPF,.RPC,.RPD,.RPL, RPG,.RMG,.RPA,.RPS) to be generated. These templates are used in ChromStar for the print-outs of chromatograms and results lists. The menu procedures of the main window are described in chapter 2.. The toolbar with ist buttons allows easy access to the main functions of the program (description in chapter 3) Chapter 4 describes the objects used for build-up a print report template. Chapter 5 describes how to create a template.

5 Report Editor - 2. Menu Procedures Page Description of the menu procedures After activating the Report Editor the main window with its menu procedures File View Help appears on the screen. File and New open a window in which a new template can be created. Different types of templates are available: Report Document.RPF for the representation of chromatograms (and results lists) Report Compare Document.RPC for the representation of multiple chromatograms in ChromStar, Compare or in multiple channel data acquisition Report 3D Document.RPD 3D representation of multiple chromatograms Report List Document.RPL for the representation of results lists Report GPC Document.RPG for the representation of chromatograms after GPC evaluation Report GPC Overlay Document.RMG for the representation of a GPC overlay plot Report DAD Document.RPA for the representation of DAD data Report Spectrum Document.RPS for the representation of spectra After clicking OK a window with the title Report1 appears in which the document is built-up. The toolbar contains buttons for easy use of the program (Toolbar, cp. chapter3). The menu bar contains additional procedures which are described in the following chapters. Using File and Open an already existing template can be opened after choosing the file type under file format and the name in the file list. Print Setup... opens a box in which the printer setup can be changed. 1-4 list the names of the 4 last used templates. Exit closes the Report Editor program. Chapter 2.1 contains a further description of the menu procedure File. View with its sub-menu procedures Toolbar and Statusbar switches the display of the toolbar and of the status bar on or off (cp. chapter 2.5). Help with its sub-menu procedures Index, Using Help and About Report Editor... opens the on-line help pages (cp. chapter 2.7).

6 Page File - Report Editor 2.1 File After choosing an already existing template or when creating a new template the menu procedure File changes ist sub-menu procedures. Additionally, the toolbar appears. The meaning of the buttons is described together with the menu procedures which they represent. New opens a window in which a new template can be created (cp. p. 2-1). Open... opens an already existing template after choosing the name in the file list. Close closes a template. If any changes were made, the question appears whether the changes are to be saved. Save saves the template. Save As... saves the template under a new name. File View Report File opens a file select box with a preview, in which an existing report file can be chosen. This file is shown in a table. Report Template opens a file select box with a preview, in which an existing template can be chosen. Slice File opens a file select box with a preview, in which an existing chromatogram can be chosen. Export... transfers a template with chromatogram in a Windows meta file (.WMF), which can be used in other applications, like word processing, drawing data base and spread sheet software. Print prints-out the generated template. Print Preview shows a preview of the template as it appears when printed-out (cp. chapter 3). Print Setup... opens a box in which the printer setup can be changed etc. lists the names of the 4 last used templates. Exit closes the Report Editor program.

7 Report Editor Edit Page Edit The menu procedure Edit offers a series of functions as are used in other WINDOWS text processing programs. Undo cancels the last entry. Cut cuts out a text. Copy copies a text into the clipboard. Paste pastes text cut out or copied by Cut or Copy. Insert inserts an object. The cursor appears as INS. Then an object is chosen out of the list of objects. The cursor appears as The cursor is moved to the place in the document where the object should be inserted. This is achieved by a mouse click. Delete deletes an object.

8 Page Format - Report Editor 2.3 Format The menu procedure Format allows the change of the page size and of the font size for the whole document. Page Setup... opens an entry box, where the upper margin, the left margin and the paper length (in mm) can be determined. Page header and page footer can be chosen. The values shown in the box correspond to the actual template. Font... determines the font type and size for the whole document. Objects can be formatted individually by Tools, Font....

9 Report Editor Tools Page Tools Tools offers various facilities to change the appearance of objects. The object to be changed must be activated previously by clicking into it, indicated by the black squares in the corners and the middle of the sides. Lock locks objects which belong together like frame, description and value, thus they can be moved together, a locked object can be unlocked. Tools For some objects an entry box can be opened, in which additional entries about the representation of the object can be made. Border creates a frame around a chosen object. Using Border Width the line width of the frame can be changed (from 0-5). Using Border Color the color of the frame can be changed. Left formats the text in a text box to the left margin. Centered centers the text in a text box. Right formats the text in a text box to the right margin. Underlined underlines the chosen text. Bold formats a chosen text in bold letters. Font... opens an entry box, in which the font type and size of a text can be determined for a chosen object. Text Color opens an entry box, in which the font color of a text can be determined for a chosen object.

10 Page View - Report Editor 2.5 View View offers facilities for the representation of the document on the screen. Document shows the main page of the template, this can be edited when shown on the screen. Page Header shows the page header, this can be edited when shown on the screen. The content of the page header is printed out on top of each page. Page Footer shows the page footer, this can be edited when shown on the screen. The content of the page footer is printed out at the bottom of each page. Grid... opens an entry box, in which a grid with a certain grid width can be laid over the document on the screen (Visible must be ticked). This facilitates the entry of the objects into the document. Testfile... opens a file select box, in which the name of a chromatogram can be chosen. This will be shown in the template in the object Chromatogram as an example. Depending on the file type chosen there are further possibilities of file selection: Multi Testfile... opens a file select box, in which for the representation of multiple chromatograms these can be chosen. For a testing print-out an offset and an angle can be determined. DAD... allows the representation of a DAD data file. In the object 2D DAD or 3D DAD. Spectrum... allows the representation of a spectrum in the object Spectrum. Multi Spectrum... allows the representation of multiple spectra in the object Multi Spectrum. Toolbar switches the tool bar on or off. Statusbar switches the status bar on or off.

11 Report Editor Window Page Window Window and its sub-menu procedures corresponds to to the menu procedure in other WINDOWS applications. New Window opens a new window and copies the content of the first window into it. Cascade lays the windows in a cascade over each other. Tile lays the windows side by side. Arrange Icons orders the icons at the bottom of the report editor window etc. lists all opened templates and allows quick acces by clicking into it.

12 Page Help - Report Editor 2.7 Help Help and its sub-menu procedures offer on-line help. Index opens the help pages. Using Help explains how to use the help pages. Context Help opens the corresponding help page, e. g. the arrow with the question mark can be moved on one of the tool buttons, by clicking the corresponding help page appears (if existing). Search opens the index of the help pages and the searching routine. About Report Editor... opens a box with information about version number and date of origin of the program.

13 Report Editor - 3. Toolbar Page Toolbar The tool bar on top of the document contains a series of buttons which allow quick access to various functions. To each button belongs a corresponding menu or sub-menu procedure. In the middle of the toolbar is a box, where, on clicking into the downwardsarrow, a list of objects appear. The objects are explained in chapter 4. By clicking into one of the buttons an explanation of it appears in the status bar. The buttons correspond to the menu procedures: File, New opens a window in which a new template can be created. File, Open... opens an already existing template after choosing the name in the file list. File, Save saves the template. File, Print prints-out the generated template. File, Print Preview shows a preview of the template as it appears when printedout.

14 Page Toolbar - Report Editor The function buttons have the following meaning: Print prints out the template. Next Page turns to the next page. Prev. Page turns to the previous page. One Page switches from double page representation to single page on the screen Two Pages switches to double page representation. Zoom in or clicking into the magnifying glass allows two step magnification. Zoom out cancels the magnification. Close closes the print preview. The operational mode of the report editor is reached again. Edit, Cut cuts a chosen text. Edit, Copy copies a chosen text into the clipboard. Edit, Paste pastes a cut out or copied text. Name of an object, the downwards arrow opens a list with all available objects (cp. chapter 4). View, Page Header shows the header of the template. The content of the header appears on top of each page. View, Document shows the main document of the template. View, Page Footer shows the footer of the template. The content of the footer appears at the bottom of each page. Tools, Border draws a frame around a chosen object. Tools, Bold formats a chosen text in bold letters. Tools, Underlined underlines the chosen text. Tools, Left formats the text in a text box to the left margin. Tools, Centered centers the text in a text box. Tools, Right formats the text in a text box to the right margin.

15 Report Editor - 3. Toolbar Page 3-3 Tools, Lock locks several objects which belong together like frame, description and value, thus they can be moved together, a locked object can be unlocked. Tools, Tools. For some objects an entry box can be opened, in which additional entries about the representation of the object can be made. Edit, Insert inserts an object. The cursor appears as INS. After an object has been chosen out of the list of objects the cursor appears as. The cursor is moved to the place in the document where the object should be inserted. This is achieved by a mouse click. Help, Context Help opens the corresponding help page, e. g. the arrow with the question mark can be moved on one of the tool buttons, by clicking into it the corresponding help page appears (if existing). Help, About Report Editor... opens a box with information about version number and date of origin of the program. File, Exit closes the Report Editor program.

16 Page Toolbar - Report Editor

17 Report Editor - 4. Objects Page Objects The report template is created by selecting different objects. All available objects are listed and can be accessed by using the scroll bar on the right. This list appears after using the downwards arrow in the box in the tool bar. The objects, which should appear in the template, are clicked one after the other. There are different types of objects: 1. Text boxes 2. Parameters 3. Chromatograms 4. Results lists 5. Control elements The text box represents a given text, which can be changed when editing the template. It will appear afterwards in all print-outs which use this template, e.g.. objects Text, Title. The parameter objects contain two text boxes: 1. the text describing the content of the following box, e.g. the object Chromatogram Name: Chromatogram Name). This text can be changed, e.g. in Name of the Chromatogram:. 2. This box is filled with X, in the print-out it will be replaced by the information given by the ChromStar program. For the representation of chromatograms the objects Chromatogram or Multi Chromatogram are used. The results lists are represented using the object Table. Control elements are: New Page, Label and Go To. New Page creates a page break. Label and Go To are used, when in one template a series of chromatograms is to be printed (e.g. with multi-channel data acquisition or in the ChromStar application Reprocess II-Compare). All objects between Label and Go To are repeatedly printed until all chromatograms are processed. Depending on its position the cursor changes its appearance and allows various operations with the objects. Above a corner of a box the cursor appears as inclined arrow. After clicking the mouse key and keeping it depressed and then moving the mouse the corner can be moved, thus allowing to change the size of a box. Above the middle of one of the sides the cursor appears as horizontal or vertical arrow. After clicking the mouse key and keeping it depressed and then moving the mouse the side can be moved up or down, to the left or to the right. When the cursor appears as crossed arrows the whole box can be moved by clicking the mouse key and keeping it depressed and then moving the mouse. In any place of the document a frame can be drawn by clicking with the left mouse button, keeping it depressed and moving the mouse. Several objects can be moved together after a frame has beeen drawn around them.

18 Page Objects - Report Editor Audit Trail inserts the information gathered during chromatogram recording. This informationen can be accessed in ChromStar in Reprocess, Reconstruction or Reintegration, under View, Audit Trail. Author inserts the ChromStar user s name. This can be entered under File, Properties in the Windows program manager after activating the ChromStar icon in the command line behind chrstar.exe seperated by a blank or after starting ChromStar in the window User Login. Calculation File inserts the content of the Calculation file used during data acquisition, or for the last calculation, respectively. After clicking into the table and using Tools, Tools a box appears in which the column width and height and the font size can be changed. Carrier Gas inserts the entries made against Carrier Gas in the Document Table of the Method file after choosing Mode=GC. A box with 3 sub-boxes appears (cp. User Defined Details). Channel No. inserts the Channel-Nr. selected in ChromStar, Analysis in the box Selection. Chromatogram inserts the chromatogram. Using Tools, Tools the representation of the chromatogram can be changed by the following entries:

19 Report Editor - 4. Objects Page 4-3 In the box Format Portrait format, Landscape format and a Chart speed ( in mm/min retention time) can be chosen. After Line-Width the line width of the curve (entries from 0 to 5) can be chosen. The line width of the axes is determined against Scale Width. After Font Size Scale the size of the scale labelling (entries from 8-20) can be chosen. After Font Size Annotate the size of the peak labelling (entries from 6-20) can be chosen. In the box Annotate the labelling of the peaks by Peak Numbers, Retention Times and Peak-Names can be chosen. If more than one item is being chosen, a separating sign, e.g. a blank or a semi-colon, can be determined in the box Separator. Color Chromatogram, Color Baseline, Color Annotate and Color Scale allow colors to be chosen for the chromatogram, for the baseline, for the labelling and for the axes. By clicking into Slice File only the baseline will not be drawn in the representation of the chromatogram. Clicking into Border draws a rectangle surrounding the chromatogram. A section is chosen by entries in the boxes Ref. time axis and Ref. int. axis, which is represented in all print-outs. Only by entries against Expand, By values in ChromStar another section can be chosen. By clicking into High Resolution a highly resolved print-out is obtained (necessary with some printers). The labelling of the scales can be changed by entries in the boxes x-label and y-label. Chromatogram Name replaces the Xs by the name of the chromatogram chosen under Data Files in the Run Table of the Method file followed by the current number. The whole box can be unlocked by Tools, Lock. After clicking in the Xs and using Tools, Tools a select box appears where you can choose whether the name of the chromatogram should be printed with (clicking in directory) or without directory (clicking in name). Color Line inserts a colored line. The color of this line corresponds to the color of the chromatogram chosen in the object multi-chromatogram. Using Tools, Tools the line width can be changed from 0 to 5. When using the object Multi-Chromatogram the correct colors are automatically correlated to the chromatograms within a loop (s. below, Label and Go To). Column Details inserts the entries made against Column details in the Document Table of the Method file. A box with 4 sub-boxes appears (cp. User Defined Details). The parameters Column Length (in mm) and Solvent Peak (in min) are used for calculating column coefficients. The preset value for the column length is mm, the preset value for the solvent peak is 1.00 min. Concentration Level inserts the concentration level entered in the Sample Table of the Method file used when recording the calibration chromatogram.

20 Page Objects - Report Editor Current Date inserts the current date and time. Two boxes appear, these can, partly or completely, be changed, moved or deleted. Current User inserts the user s name entered on starting ChromStar. Data Handling File inserts the content of the Data handling file used during data acquisition, or for the last calculation, respectively. After clicking into the table and using Tools, Tools a box appears in which the column width and height and the font size can be changed. Date inserts 6 boxes with date and time of the data acquisition, date and time of the last integration and date and time of the last calculation. These can, partly or completely, be changed, moved or deleted. Detector inserts the entries made against Detector in the Document Table of the Method file after choosing Mode=GC. A box with 2 sub-boxes appears (cp. User Defined Details). Detector Details inserts the entries made against Detector Details in the Document Table of the Method file. A box with 4 sub-boxes appears (cp. User Defined Details). Emission inserts the value of the emission wavelength of a fluorescense detector, as entered either as preset value in the Preset Table or in the LC procedure file at the time 0. File inserts the content of a text file. After using Tools, Tools in an entry box can be chosen whether the file should be deleted after the print-out or not. Go To ends a loop. In the representation of multiple chromatograms all objects between Label and Go To are shown until the number of chosen chromatograms is extinguished. Using Tools, Tools the loop number can be changed. Inj. No. inserts the number of injection, which was active when recording the chromatogram. The number of injections is defined in the autosampler table of the method file under Inj., these are carried out one after the other out of one vial. Inj. Volume inserts the injection volume as entered under Vol in the autosampler table of the method file. This is not identical with the injection volume entered in the Document Table under User Defined Details, since when using the autosampler in each line of the Autosampler Table another volume can be entered and will be used by the autosampler.

21 Report Editor - 4. Objects Page 4-5 Injector inserts the entries made against Injector in the Document Table of the Method file after choosing Mode=GC. A box with 3 sub-boxes appears (cp. User Defined Details). Label starts a loop. In the representation of multiple chromatograms all objects between Label and Go To are shown, until the number of chosen chromatograms is extinguished. A document can have more than one loop. Label and Go To belonging to one loop must show the same number. Using Tools, Tools the loop number can be changed. LC-Procedure inserts the content of the LC procedure file used during data acquisition. After clicking into the table and using Tools, Tools a box appears in which the column width and height and the font size can be changed. Line inserts a line in the template. Using Tools, Tools the line width can be changed from 0 to 5. Using Line Color the color can be changed. Linear Velocity inserts the linear velocity in mm/s, this is calculated according to: column length (in mm) / (retention time of solvent peak*60). The column length and the solvent peak are enterd in ChromStar in the Methodfile in the Document page or in Reprocess, Calculation in the box Update Input Values. Logo. After clicking into Logo in the list of objects a file select box appears, in which a.bmp file can be chosen. This picture appears in a box and is automatically inserted in the document. Mobile Phase inserts the entries made against Mobile Phase in the Document Table of the Method file. A box with 5 sub-boxes appears (cp. User Defined Details).

22 Page Objects - Report Editor Multi Chromatogram inserts the three-dimensional representation of multiple chromatograms. A box appears where parameters for the display can be determined, also on clicking into Tools, Tools. In order to enter the names of multiple chromatograms in the template, the loop Label and Go To must be inserted. The following entries and changes can be made: In the box Common Parameters the format is chosen as Portrait or Landscape. The Line Width can be chosen from 0 to 5. Border lays a frame around the chromatograms. Font Size Scale and Font Size Annotate determine the size of the scale and peak labelling. Reference time axis and Reference intensity axis determine a section of the chromatograms to be displayed. Only by entries against Expand, By values in ChromStar another section can be chosen. Hide Background Lines: Only the chromatogram in front is drawn in a continuous line. Data Point: Each data point is represented as a vertical line. In the boxes Individual Parameters, selected by the numbers from 1-10 to the right, parameters for the representation of the individual chromatograms are chosen. Under Annotate the labelling by peak numbers, retention times and peaknames can be chosen. The separating sign is chosen in the box after Separator. Slice File only must not be clicked when labelling peaks. For the annotation by peak names, a calculation file containing the peak names must be used for the calculation. In the boxes Color Chromatogram and Color Baseline the color of the chromatogram and of the baseline can be chosen.

23 Report Editor - 4. Objects Page 4-7 New Page inserts a page break. Noise inserts the Noise value entered either as preset value in the Preset table or in the Integration Page of the Data-Handling-file at the time 0. Notes inserts the entries made against Notes in the Run Table of the Method file. Oven inserts the entries made against Oven in the Document Table of the Method file after choosing Mode=GC. A box with 3 sub-boxes appears (cp. User Defined Details). Page No. inserts two boxes with the current page number and the number of pages of the whole document. These boxes can be part of the page header or footer. Parameter Files inserts the names of the files used when recording the chromatogram, these are determined in the Run Table of the Method file or when using an autosampler in the Autosampler Table, respectively, and in the data handling file. Peak Parameter inserts the Peak-Parameter- value entered in the Acquisition Page of the Data-Handling-file. Sample Identifier inserts the entry made against Sample Id. in the Sample Table of the Method file. Sample Info inserts the Sample Info entered in the Sample Table of the Method file. Set ChannelNo correlates the channel number chosen to the following object. Using Tools, Tools the channel number can be changed. This object can be used for the representation of several chromatograms in one print-out and should therefore be saved in a.rpc or.rpd report print template. A template with this object can also be used in Reprocess II, Compare or 3D. The template should only contain as much Set ChannelNo objects as chromatograms are to be printed. When recording several chromatograms in Analysis the labelling of the axes used for recording the chromatogram chosen under Channel Properties is then used in the printout of the chromatogram according to the channel number. Skim Ratio inserts the Skim Ratio value entered either as preset value in the Preset table or in the Integration Page of the Data-Handling-file at the time 0. Slice Width inserts the Slice Width entered in the Acquisition Page of the Data- Handling-file. Solvents inserts the initial solvent composition as entered in the LC procedure file at the time 0.

24 Page Objects - Report Editor Table inserts the results list. A box appears in which details about the representation of the table can be determined. This box also appears when an existing table in the document is activated on clicking Tools, Tools. In the box Available Items the columns of the table can be chosen. By using the double arrow they are transferred into the box Selected Items. The selection of more than one item is done according to the Windows convention by using the Shift- or Ctrl-key. The column header can be changed individually in the box Head Text after clicking it in the box Available Items (when using Autoselect, s. below) or Selected Items. In a two line header the line feed is achieved by using Ctrl-N.

25 Report Editor - 4. Objects Page 4-9 The following columns can be used in the table: Peak No. Peak number Window No. Number of the peak in the peaks list of the Calculation file Retention Time Peak Start Peak End Area Peak area Height Peak height Area0/Recovery Peak area/recovery (entered in the Calculation file in the Peak table under Area0/Recovery%) Response Factor Response factor for the evaluation of quantitative amounts (after averaging the response factors: K1 in the Regression Table of the Calculation file) Amount Amount of the standard in a calibration Absolute Result of the quantitative evaluation The calculation unit is automatically entered in a line beneath the column header, as entered against Calculation Units in the Calculation file. Relative Ratio of weighed-in amount of internal standard to calculated amount of internal standard, is only calculated when Calculation Method = Internal Standard If the Internal Standard method is used but not Auto-Select, the column Mode should be part of the table where the internal standard is marked as IS. When using Auto-Select the label IS appears in the column Window No. Weight percentage of the components of the whole amount of sample, entered in Reprocess, Calculation in the box Update Input values or in the Method file in the Sample Table Area% Area percent Name Peak name Bandwidth Half height bandwidth Asymmetry Peak asymmetry Platenumber Plates/m Plates per Meter HETP Plate height (in µm) Resolution calculated for two adjacent peaks K K -value (Capacity) Selectivity Calculated for two adjacent peaks Mode Additional information for a peak as IS (for internal standard) or M (for manually integrated), if not Autoselect was chosen. If the report file does not contain all the calculation in the user defined table the missing results are filled by 0.

26 Page Objects - Report Editor The colors and the line width of the lines in the table are determined using Line Color and Line Width. Using Auto-Select the calculation carried out last and stored in the report file is represented in the table. The calculation mode appears above the table.if the user wants to determine the contents of the table Autoselect must be deselected. With Custimized Headers the user defined headers are chosen. The Font Size can be chosen from The Row Width (mm) can be chosen from The Column Width can be chosen from -20 to + 20). A value between 0 and 3 is recommended. Using All, Head or Body the whole table, the header or only the body is chosen for the print-out. In the boxes below the automatically generated texts as calculation type or other remarks can be changed. Text inserts a text box, in which a test can be entered. The test e.g. can be part of the page header and will appear on top of each page. By double click in the box the cursor appears at the beginning of the text, this can now be changed or a new text can be entered. The same happens on clicking into Tools, Tools. When entering a text do not use the del key to delete a character, but instead of this use the backspace-key. Threshold inserts the Threshold value entered either as preset value in the Preset table or in the Integration Page of the Data-Handling-file at the time 0. User Defined Details inserts the entries made against User defined details in the Document Table of the Method file. A box with 4 sub-boxes appears, these can, partly or completely, be changed, moved or deleted. The correlation of the contents of the method file to the print-out still remains. User Level inserts the User Level when using ChromStar under GLP-conditions. Using Tools, Tools the individual descriptions of the user levels can be changed. Vial No. inserts the number of the autosampler vial, out of which the injection for the chromatogram was made. The vial number was entered in the autosampler table of the method file under Vial. Wavelength inserts the value of the wavelength of a UV detector or the excitation wavelength of a fluorescense detector, as entered either as preset value in the Preset Table or in the LC procedure file at the time 0.

27 Report Editor 4. Objects Page 4-11 If under File, New the type Report DAD Document.RPA or Report Spectrum Document.RPS is chosen, the list of objects available will be changed offering additional objects for the print-out of a DAD data file or a spectrum. Using the template type *.RPA a chromatogram can be printed as well, all objects for the print-out of a chromatogram are available. This can be used in Analysis DAD, when immediately after recording DAD data, the DAD data are to be printed out together with a chromatogram. Additional objects are available for the template type *.RPA. 2D DAD inserts a density plot into the template. Using Tools, Tools or representation can be changed in the window shown below. Under Reference minute axis and Reference nm axis time and wavelength values can be changed and a section of the data set is displayed. The annotation of the axes can be changed in the boxes Label X and Label Y. the 3D DAD displays a 3D Plot of the data file. Using which the representation can be changed. an entry box appears, in The key View Angle opens a window in which the view angle can be changed by using the mouse.

28 Page Objects Report Editor DAD Filename inserts the name of the DAD data file. The whole box can be unlocked by Tools, Lock. After clicking in the Xs and using Tools, Tools a select box appears where you can choose whether the file name should be printed with (clicking in fullpath) or without directory (clicking in filename). Document Page inserts the information given in Reprocess, 2D, File, View Document Page. These entries were made in the Documentation Page of the method file. File Information inserts the following information: Scan Parameter inserts the information given in Reprocess, 2D, File, View Scan Parameter. These entries were made in the Scan Parameter file previously to DAD data recording. The report print template *.RPS offers several objects with spectra specific information. Document Page inserts the information given in Reprocess, Spectrum, Spectrum, Information in the part Documentation Page. These entries were made in the Documentation Page of the method file. File Information inserts the information given in Reprocess, Spectrum, Spectrum, Information in the part File Information. Scan Parameter inserts the information given in Reprocess, Spectrum, Spectrum, Information in the part Scan Parameter. These entries were made in the scan parameter file.

29 Report Editor 4. Objects Page 4-13 Multi Spectrum inserts a box for the representation of multiple spectra. Using Tools, Tools an entry box is opened where details of the representation can be changed. Common Parameters for all spectra are e.g. portrait or landscape format, or the section to be displayed. Individual Parameters are e. g. the annotation of a spectrum with wavelengths (Peak Numbers) or the color. The annotation appears at a band maximum. Spectrum Filename inserts the spectrum filename. After unlocking the box, clicking in the right hand part and using Tools, Tools a box appears where you can choose whether the name is printed-out with or without directory. When using the object Multi Spectrum the names of the spectra can be listed in a loop consisting of object Label, object Spectrum Filename, object Goto. The color correlated to a spectrum can be shown in the same loop with the object Color Line.

30 Page Objects Report Editor Spectrum inserts the representation of a spectrum. Using Tools, Tools a box is opened where the details of the representation can be changed. Under Format the portrait or landscape format can be chosen. By clicking in Wavelengths under Annotate the band maxima are annotated with wavelengths. Under Reference... a section can be chosen.

31 Report Editor - 4. Objects Page 4-15 If under File, New the type Report GPC Document.RPG or Report GPC Overlay Document.RMG was chosen, the list of objects is changed. Additionally, it contains special GPC objects thus allowing the print-out of GPC evaluation. Baseline Mode specifies how the baseline was drawn (horizontal or trapezoidal). Baseline drawn enters start and end time of the baseline in two boxes. Integration drawn enters the evaluation range in two boxes. Total Area enters the total area of the evaluation range. Nr. of Data Points enters the number of data points (slices). Av. Molecular Weight enters the average molar masses (Mn, Mw, Mz, Mz+1, D) in 5 boxes. Calibration Mode specifies the kind of calibration (narrow or broad). Parameter Files enters the names of the GPC data handling file (.GIN) and of the GPC calibration file (.GCA) used for evaluation. Mark-Houwink-Const. enters the Mark-Houwink constants used for evaluation. Ret. of Ref. Peak enters the retention time of the reference peak as entered in the GPC calibration file. Ret. of Win. Peak enters the percent time window of the reference peak. Coefficients enters the coefficients K0 to K9 of the GPC calibration file in 10 boxes. A box can be removed, e.g. if a coefficient is 0.

32 Page Objects - Report Editor Distribution Plot enters the distribution plot of molar masses into the document. Using Tools, Tools the representation of the distribution plot can be changed. Portrait or landscape format is chosen in the box Format. The annotation Moments, Molecular Weight and Cumulative % is chosen in the box Annotate. When using Moments the letters used for the labelling (Mn, Mw, etc.) can be altered. Border draws a frame around the distribution plot. Integral switches the integral distribution curve on or off. Log. Scale switches the scaling of the x-axis from logarithmed to de-logarithmed scaling. Grid Horizontal and Grid Vertical lay a grid over the distribution plot. Using Line Width and Scale Width the line width of the curve and of the axes can be changed. Using Font Size Scale and Font Size Annotate the font size of the annation of the axes and of the curve can be changed. After clicking the buttons Color Distribution,.. Integral,...Annotate,...Scale or... Grid a box appears in which the color can be chosen individually. For the representation of multiple distribution curves Overlay Plot must be clicked. Values for Offset and Angle can then be entered. Using Hide Background Lines the curves overlaid by other curves are not displayed. The labelling of the vertical axes can be changed in the boxes after Y-Label 1 und Y- Label 2. The kind of distribution curve (Mn, Mw, etc.) is chosen in the box Plot. If Use ChromStar Styles is clicked, the representation of plot and scaling as entered in ChromStar is printed-out.

33 Report Editor - 4. Objects Page 4-17 Data Handling File enters the content of the GPC data handling file. After clicking into the table and using Tools, Tools a box appears in which the column width and height and the font size can be changed. Calibration File enters the content of the GPC calibration file. The table can be changed. as described under Data Handling File. Chromatogram enters the chromatogram together with the calibration values. Using Tools, Tools the representation of the chromatogram can be changed. Table adds the results list of the GPC evaluation into the document. Using Tools, Tools the column width, the row width and the font size can be changed. The table contains the columns Time (Retention time), Mol.Wt/Slice (molar mass of the slice according to the calibration), Slice Area, Area %, Cumulative Area, Cumulative Area %. The length of the frame determines how long the table will be (depending on this will be the comprehensiveness of molecular mass analysis). If Short is not ticked, the GPC report will be printed in full length.

34 Page Objects - Report Editor Ext.-Std.-Calculation opens a box with the results of a quantitative GPC evaluation and the entries made in the GPC calibration file if External Standard was marked. The parameter boxes can be changed, moved or deleted after being activated. The describing texts can be changed, as well. Summary Table adds a table, if in the GPC data handling file more than one evaluation range was entered. The box above the table contains details of the chromatogram and of the files used. After being activated the boxes can be changed, moved or deleted. The describing text can be changed, as well. The table contains time limits of the subranges, the areas and the area in %, where the first range is set to 100 %.

35 Report Editor - 5. How to use the Report Editor Page How to use the Report Editor 5.1 Creating a Template (.RPF) The creating of a template will be described step by step. 1. Activate the report editor program by double click. 2. Click in File and New. Confirm Report Document with OK. 3. Creating the Page Header Click into the tool bar button or use View, Page Header. The page header should contain the name of the chromatogram without path and the actual date. Click into the downwards arrow and choose Chromatogram Name and Current Date out of the list of objects. The appropriate boxes appear in the window Report 1. The Time box is active (marked by the black squares) and is deleted by Edit, Delete or by the DEL key. The Chromatogram Name box is activated by clicking into it and is unlocked by Tools, Lock. The sub-boxes can be changed and moved after being avtivated. A double click allows the text in the text box to be edited. After activating the XXX in the Chromatogram Name box, the print-out of the name of the chromatogram without path is determined by clicking into Tools, Tools and clicking after Name. The size of the surrounding box is fitted and locked by Tools, Lock. The Date box is activated by clicking into it and is unlocked by Tools, Lock. The sub-boxes can be changed and moved after being avtivated. A double click in the text box allows the text to be edited. The size of the surrounding box is fitted and locked by Tools, Lock.

36 Page Creating a Template - Report Editor 4. Creating the Page Footer Click into the tool bar button or use View, Page Footer The page footer should contain page number. Click into the downwards arrow and choose Page Nr. out of the list of objects. The Page and of boxes appear in the window Report 1. The boxes are activated by clicking into them and unlocked by Tools, Lock. The boxes can be changed and moved. A double click in the text box allows the text to be edited. 5. Creating the Main Page Click into the tool bar button or use View, Document. The main document shoul contain: The user s name, the notes entered in the Run Table of the Method file, the Sample Identifier (entered in the Sample Table of the Method file, the text: Data acquisition parameters, the Slice Width and Noise parameters entered in the Data Handling file, the names of the files used during data acquisition as entered in the Method file, the chromatogram and the results list. After clicking into the downwards arrow the following objects are choosen out of the list of objects: Author, Notes, Sample Identifier, Text, Slice Width, Noise, Parameter Files, Chromatogram, Table (in the entry box the parameter Autoselect is marked, the box is quitted by OK). When Autoselect is marked, the results list contains the results as calculated by the calculation file chosen. Thus the difficult procedure of editing a results list is avoided. All calculated results are printed-out. After activating and unlocking by Tools, Lock the boxes can be changed. Diminuish the Notes box and move the following boxes into the free space. Activate the chromatogram and click into the tool button Tools, Tools. In the entry box under Annotate choose the labelling Retention Times and Peak Names, Separator : three blanks. Using Format, Page Setup the left margin, the upper margin and the page length are defined as: 15, 5, 285. Using Format, Font... the font is chosen, in this case Arial, Standard, 11. The results list must be formatted in smaller fonts, so that all columns of the table appear on the paper printed-out. The table box is activated by clicking into it. Using Tools, Font... the font size is changed appropriately. Using View, Testfile... and selecting a chromatogram out of the file list as example it can be represented on the screen.

37 Report Editor Creating a Template Page 5-3 Click into File, Print Preview to get the print preview on the screen. The created template is saved via File, Save As... and entering a name. After clicking into File, Print one page is obtained as print-out.

38 Page Representation of multiple Chromatograms - Report Editor 5.2. Creating a template for the representation of multiple chromatograms 3D-display of a series of chromatograms To print out multiple chromatograms in ChromStar, Display Chromatograms, 3 D, a template with at least the following objects must be created: 1. Label 2. Chromatogram Name 3. Go To 4. Multi Chromatogram This template is saved in a file with the file extension.rpd. Representation of a series of chromatograms using Compare To print out multiple chromatograms in ChromStar, Display Chromatograms, Compare, a template with at least the following objects must be created: 1. Label 2. Chromatogram Name 3. Chromatogram 4. Go To This template is saved in a file with the file extension.rpc.

39 Report Editor Examples Page Examples To characterize a template, some important details are necessary. These are listed in the following together with the print-out. Name: SHORT.RPF Page Setup: 10/5/285 Font: Courier New Standard 12 Page header: Chromatogram name + date Page footer: Page number Main page: Chromatogram (Portrait, Annotate Ret. Times) Table (autoselect), Font 11

40 Page Examples - Report Editor Name: RECONST1.RPF Page Setup: 10/5/285 Font: Courier New Standard 12 Page header: Chromatogram name + date Page footer: Page number Main page: Chromatogram (Portrait, without baseline)

41 Report Editor Examples Page 5-7 Name: RECONST2.RPF Page Setup: 10 / 5 / 285 Font: Courier New Standard 12 Page header: Chromatogram name + date Page footer: Page number Main page: Chromatogram (Landscape, without baseline)

42 Page Examples - Report Editor Name: TABLE.RPL Page Setup: 10/5/285 Font: Courier New Standard 12 Page header: Chromatogram name + date Page footer: Page number Main page: Table (autoselect), font 11

43 Report Editor Examples Page 5-9 Name: MULTI3.RPD Page Setup: 15 / 5 / 280 Font: Arial 11 Page header: Author + date Page footer: Page number Main page (2 DIN A4-pages): 1st loop: Chromatogram name + line Multi-Chromatogram (Portrait, with baseline) 2nd loop: Chromatogram name + table (Autoselect)

44 Page Examples - Report Editor When using a color printer the correlation of chromatogram name and line is printed-out in colors.

45 Report Editor - 6. Index Page Index A About Report Editor 2-8, 3-3 Absolute 4-9 Amount 4-9 Angle 4-6, 4-11, 4-16 Annotate 4-3, 4-6, 4-13, 4-16 Arrange Icons 2-7 Asymmetry 4-9 Audit Trail 4-2 Author 4-2 Auto-Select 4-10 Av. Molecular Weight 4-15 Available Items 4-8 B Baseline Mode 4-15 Baseline drawn 4-15 Bandwidth 4-9 Bold 2-5, 3-2 Border 2-5, 3-2, 4-3, 4-6, 4-12, 4-17 Border Width 2-5 Border Color 2-5 C Calculation File 4-2, 4-7 Calibration File 4-13 Calibration Mode 4-15 Capacity Carrier Gas 4-2 Cascade 2-7 Centered 2-5, 3-2 Channel No. 4-2 Channel properties 4-7 Chart Speed 4-2, 4-17 Chromatogram 2-6, 4-2, 4-9, 4-17 Chromatogram Name 4-3 ChromStar 1-2 Close 2-2 Coefficients 4-11 Color Annotate 4-3, 4-16 Color Baseline 4-3, 4-6 Color Chromatogram 4-3, 4-6, 4-17 Color Distribution 4-16 Color Grid 4-16 Color Integral 4-16 Color Line 4-3 Color Scale 4-3, 4-16 Column Details 4-3 Concentration Level 4-3 Context Help 2-8, 3-3 Copy 2-3, 3-2 Current Date 4-4 Current User 4-4 Customized Headers 4-10 Cut 2-3, 3-2 D 2D DAD D DAD D Plot 4-11 DAD DAD Data file 4-11 DAD Filename 4-12 Data-Handling-File 4-4, 4-7, 4-7, 4-17 Data Point 4-6 Date 4-4 Delete 2-3 Density Plot 4-11 Detector 4-4 Detector Details 4-4 Distribution Plot 4-16 Document 2-6, 3-2 Document Page 4-12

46 Page Index - Report Editor E Edit 2-3 Emission 4-4 Exit 2-1, 2-2, 3-3 Export 2-2 Ext.-Std.-Calculation 4-18 F Factor 4-9 File 2-1, 2-2, 4-4 File Information 4-12 File View 2-2 Font 2-4, 2-5 Font Size 2-4 Format 2-4 G Go To 4-4, 5-4 GPC-Data-Handling-File 4-15, 4-17, 4-18 GPC-Calibration-File 4-15, 4-17, 4-18 Grid 2-6 H Head Text 4-8 Help 2-1, 2-8 HETP (Theoretical plate height) 4-9 Hide Background Lines 4-6 I Index 2-8 Injector 4-4 Inj Volume 4-4 Insert 2-3, 3-3 Integration drawn 4-15 K K -value 4-9 L Label 4-5, 4-13, 5-4 Landscape 4-2, 4-6, 4-13, 4-17, 5-7 LC-Procedure 4-5 Left 2-5, 3-2 Line 4-5 Linear Velocity 4-5 Line Color 4-9 Line Width 4-2, 4-9 Lock 2-5, 3-3 Logo 4-5 M Mark-Houwink-Const 4-15 Method-File 4-2, 4-3, 4-7, 4-8, 4-12 Mobile Phase 4-5 Mode 4-9 Multi Chromatogram 2-6, 4-3, 4-4, 4-5, 5-4 Multi Spectrum 2-6, 4-13 Multi Testfile 2-6, 4-6 N New 2-1, 3-1 New Page 4-6 New Window 2-7 Noise 4-6 Notes 4-6 No. of Data Points 4-15

47 Report Editor - 6. Index Page 6-3 O Object 2-3, 2-5, 4-1 Offset 4-6, 4-16 Open 2-1, 2-2, 3-1 Oven 4-7 Overlay Plot 4-16 P Page Footer 2-4, 2-6, 3-2, 5-2 Page Header 2-4, 2-6, 3-2, 5-1 Page No. 4-7 Page Setup 2-4, 2-6 Parameter Files 4-7, 4-15 Paste 2-3, 3-2 Peak Parameter 4-7 Platenumber 4-9 Plot 4-16 Portrait 4-2, 4-6, 4-13, 4-17, 5-6 Print 2-2, 3-1, 5-3 Print Preview 2-2, 3-1 Print Setup 2-1, 2-2 R Recovery 4-9 Reference intensity axis 4-3, 4-6, 4-17 Reference time axis 4-3, 4-6, 4-17 Relative 4-9 Report Compare Document (.RPC) 2-1, 5-4 Report Document (.RPF) 2-1 Report 3D Document (.RPC) 2-1, 5-4 Report File 2-2, 4-8 Report DAD Document (.RPA) 2-1, 4-11 Report GPC Document (.RPG) 2-1, 4-15 Report GPC Overlay Document (.RMG) 2-1, 4-15 Report List Document (.RPL) 2-1 Report Spectrum Document (.RPS) 2-1, 4-11 Report Template 2-1, 2-2, 2-6, 4-9, 5-1, 5-4, 5-5 Resolution 4-9 Response factor 4-9 Results list 4-8 Ret. of Ref. Peak 4-15 Ret. of Win. Peak 4-15 Right 2-5, 3-2 S Sample Identifier 4-7 Sample Info 4-7 Save 2-2, 3-1 Save As 2-2, 5-3 Scan Parameter 4-12 Scan parameter file 4-12 Search 2-8 Selected Items 4-8 Selectivity 4-9 Set ChannelNo. 4-7 Skim Ratio 4-7 Slice File 2-2 Slice File only 4-3, 4-6 Slice Width 4-7 Solvents 4-7 Spectrum 2-6, 4-11, 4-12, 4-14 Spectrum Filename 4-13 Statusbar 2-1, 2-6, 3-1 Summary Table 4-18

48 Page Index - Report Editor T Table 4-8, 4-17 Template 2-1, 2-2, 5-1, 5-4, 5-5 Testfile 2-6 Text 2-5, 4-10 Text Color 2-5 Threshold 4-10 Tile 2-7 Toolbar 2-1, 2-6, 3-1 Tools 2-5, 3-2, 3-3 Total Area 4-15 U Underlined 2-5, 3-2 Undo 2-3 Use ChromStar Styles 4-16 User Defined Details 4-10 User Level 4-10 Using Help 2-8 V View 2-1, 2-6 View Angle 4-11 W Wavelength 4-10, 4-13 Weight 4-9 Window 2-7 X X-Label 4-2, 4-14, 4-16 Y Y-Label 4-2, 4-14, 4-16