What DNA can do for you! Genetic methods and the landing obligation
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1 What DNA can do for you! Genetic methods and the landing obligation Brian Klitgaard Hansen, Gregory Farrant, Rob Ogden, Emily Humble, Guðbjörg Ólafsdóttir & Einar Eg Nielsen
2 Making discards count! Fish blocks Catching low value species and sizes is unavoidable One way of optimizing utilization of low value is to store and land them as bulk products Fish blocks Export Silage (on board) Fishmeal or fishoil Silage Difficult to control what is being landed if there are no/few signs to identify species COUNCIL REGULATION (EC) No 1224/2009 Can DNA help identify and quantify what species are in these products? 2 DTU Aqua, Technical University of Denmark
3 DNA as a tool in fisheries 3 Silage processing tank x 3 DTU Aqua, Technical University of Denmark
4 DNA as a tool in fisheries 4 Silage processing tank x x x DNA quantity biomass 4 DTU Aqua, Technical University of Denmark
5 Study species Atlantic cod (Gadus morhua - Torsk) Whiting (Merlangius merlangus - Hvilling) Haddock (Melanogrammus aeglefinus - Kuller) Atlantic wolffish (Anarhichas lupus - Havkat) 5 DTU Aqua, Technical University of Denmark
6 6 DTU Aqua, Technical University of Denmark
7 7 DTU Aqua, Technical University of Denmark
8 8 DTU Aqua, Technical University of Denmark
9 9 DTU Aqua, Technical University of Denmark
10 250µl = liter 10 DTU Aqua, Technical University of Denmark
11 11 DTU Aqua, Technical University of Denmark
12 12 DTU Aqua, Technical University of Denmark
13 13 DTU Aqua, Technical University of Denmark
14 Identifikation of all 4 species DNA detectable for 21 days Biomass proportions are inaccurate 14 DTU Aqua, Technical University of Denmark
15 Do all fish contain the same amount of DNA? 15 DTU Aqua, Technical University of Denmark
16 16 DTU Aqua, Technical University of Denmark
17 17 DTU Aqua, Technical University of Denmark
18 18 DTU Aqua, Technical University of Denmark
19 19 DTU Aqua, Technical University of Denmark
20 20 DTU Aqua, Technical University of Denmark
21 21 DTU Aqua, Technical University of Denmark
22 22 DTU Aqua, Technical University of Denmark
23 Likely explanation: relationship between biomass and DNA content are similar among codfishes For codfishes we can accurately identify species and quantify their biomass in silage Finetuning with correction factors? 23 DTU Aqua, Technical University of Denmark
24 Fish blocks: sampling methods Method I: Filter water > Extract DNA -> Analyze Method II: Swab > Extract DNA -> Analyze 24 DTU Aqua, Technical University of Denmark
25 DNA results from frozen fish blocks 25 DTU Aqua, Technical University of Denmark
26 DNA results from frozen fish blocks Both methods was able to detect the two species 26 DTU Aqua, Technical University of Denmark
27 DNA results from frozen fish blocks 27 DTU Aqua, Technical University of Denmark
28 What can DNA do for you! Identify the species present in silage and fish block Quantify biomass proportion of codfishes at high accuracy in silage Good potential for calibration among species in future pratical applications High potential for using and transferring applications to portable devices for fast onsite use by non-experts 28 DTU Aqua, Technical University of Denmark
29 Thank you for your attention Thanks to all involved With support from Kristinn Ólafsson, Gregory Farrant, Guðbjörg Ólafsdóttir, Steinunn Magnúsdóttir Rob Ogden & Emily Humble Contact information: Brian Klitgaard Hansen Rasmus Bach Ebert, Steen Knudsen & Peter Rask Møller Einar Eg Nielsen, Britta S. Pedersen, Majbritt Jacobsen, Magnus Wulf Jacobsen, Dorte Meldrup and Clara Ulrik 29 DTU Aqua, Technical University of Denmark
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