CALIBRATION of a STREAM
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1 CALIBRATION of a STREAM PRELIMINARY SET UP: STANDARD GAS 5 PSIG; FLOW METER: 20/30 CC/MIN. DIP TUBE LIQUIDS AND WELKER PISTON CYLINDERS ACCORDINGLY. ALL OPERATIONS ARE INITIATED FROM THE MAIN MENU; SELECTION OF SCREENS FOLLOWS WITH STEPS ON EACH SCREEN TO BE CARRIED OUT. KEYS FUNCTIONS: ALT+E = EDIT; ALT+F# = SELECT ANALYZER; SHIFT +F# = SELECT STREAM; CTRL +F# = SELECT METHOD; CTRL + HOME = BRINGS TO 1ST COLUMN.. I. INITIAL SET UP. >>"F" SCREEN = STREAM DEFINITION. (ADD CALIBRATION STREAM.) 1. ALT+E=EDIT; ARROW DOWN TO OPEN SPACE AND TYPE CALIBRATION. 2. TAB TO METHOD: USE LEFT AND RIGHT ARROWS TO SELECT THE METHOD. 3. PRESS "F2" TO SAVE. >>NOTE: TAB TO INTERVAL, NORMALLY 0. IF DESIRED SELECT INTERVAL. 4. PRESS "F"3 TO RETURN TO MAIN MENU. >>"G" SCREEN= STREAM SEQUENCE. ( ADD CALIBRATION STREAM.) 1. ALT+E=EDIT; ARROW DOWN TO OPEN SPACE. 2. LEFT AND RIGHT ARROWS TO SELECT STREAM. 3. PRESS "F"2 TO SAVE. 4. PRESS "F3" TO RETURN TO MAIN MENU
2 II. "J" SCREEN = STREAM ON/OFF METHOD; 1, STEP 1. ENABLE ONLY STREAM TO CALIBRATE. 2. STEP 2. KEY F2 TO SAVE. 3. STEP 3. KEY F3 TO RETURN TO MAIN MENU. III. "I" SCREEN = RUN/ STOP ANALYZER. 1. STEP 1. UP ARROW TO START CYCLIC OPERATION. > NOTE: 1 CLICK= CONTINUE PURGING; DOUBLE CLICK= START SEQUENCE. 2. STEP 2. DOWN STOP AT END OF CYCLE. 3. STEP 3. DOWN TO RETURN TO MAIN MENU.( ENTER ) 4. NOTE: THIS STEP IS TO BE CARRIED OUT AT THE COMPLETION OF EACH ANALYSIS. IV. "N" SCREEN = ANALYZER STATUS. 1. STEP 1. CHECK FOR ANALYZER CONDITION. A) ANALYZER MODE. B)OVEN TEMPERATURE. C) CYCLE TIME. D) RUN TIME. E) ALARM STATUS F) VALVE DRIVER STATUS. G) CURRENT STREAM H) CURRENT METHOD. NOTE: ANY ADJUSTMENTS SHOULD BE MADE WHEN CYCLE TIME IS OVER.
3 V.. "D" SCREEN = METHOD COMPONENTS. 1. STEP 1. KEY CTRL+F1; F2; F3; TO SELECT METHOD. 2. STEP 2. KEY ALT+KEY E= EDIT 3. STEP 3. TAB BAR TO ELUTION 4. STEP 4. ARROWS UP/DOWN TO SELECT COMPONENTS. 5. STEP 5. ENTER ELUTION TIMES 6. STEP 6. TAB BAR TO CAL. STANDARD COLUMN. 7. STEP 7. ENTER CAL.STD. VALUES. 8. STEP 8. ENTER FACTORS ( MAKE SURE TO ENTER , ON REFERENCE PEAK ONLY (PEAK OF HIGHEST CONCENTRATION ) 9.. STEP 9. KEY F2 TO SAVE. 10 STEP 10. KEY F3 TO RETURN TO MAIN MENU. VI "O" SCREEN = DETECTOR GRAPH. 1. STEP 1. SELECT ANALYZER; USE + / - KEYS TO SELECT. 2. STEP 2. PRESS ENTER. 3. STEP 3. PRESS KEY "M" = MONITOR; IT SWITCHES TO CALIBRATION STREAM. 4. STEP 4. PRESS ENTER. 5. STEP 5. TO RECORD THE CHROMATOGRAM, PRESS "R" FOR RECORDING. 6. STEP 6. RUN AT LEAST 3 CHROMATOGRAMS, (PREFERABLY 5 FOR REPEATABILITY) 7. STEP 7. WRITE ON A PIECE OF PAPER THE MV. DISPLACEMENT AND ELUTION TIME FOR EACH COMPONENT ON EACH ANALYSIS. 8. STEP 8. AT THE END OF THE ANALYSIS, PRESS "Q" TWICE, OR "F3" TWICE TO RETURN TO MAIN MENU.
4 VII. "S" SCREEN = LAST FIVE ANALYSIS. 1. STEP 1. CHECK REPEATABILITY 2. STEP 2. DECISION TO CALIBRATE* BASED ON REPEATABILITY. 3. STEP 3. KEY F3 TO MAIN MENU. VIII. "A" SCREEN = ANALYZER DEFINITION 1. STEP 1. KEY ALT + KEY E= EDIT 2. STEP 2. TAB BAR TO CALIBRATION STREAM #. 3. STEP 3. ENTER STREAM NUMBER. 4. STEP 4. KEY F2 TO SAVE. 5. STEP 5. KEY F3 TO RETURN TO MAIN MENU. IX."I" SCREEN = RUN/STOP ANALYZER. 1. ARROW TO START CALIBRATION RUN. 2. KEY "F" 3 TO MAIN MENU. X. "N" SCREEN = ANALYZER STATUS. 1. STEP 1. CHECK THAT IT SHOWS CALIBRATION. 2. STEP 2. CHECK ANALYSYS TIME IN PROGRESS 3. STEP 3. KEY F3 TO RETURN TO MAIN MENU.
5 XI. "O" SCREEN = DETECTOR GRAPH. 1. STEP 1. VERIFY PROPER ANALYZER. 2. STEP 2. PRESS ENTER. 3. STEP 3. PRESS KEY "M" MONITOR; IT SWITCHES TO CALIBRATION STREAM. 4. STEP 4. PRESS ENTER. 5. STEP 5. SELECT RECORDING TO HAVE CHROMATOGRAM OF CALIBRATION. NOTE: NEW FACTORS WILL BE REGENERATED AT THE END OF THE CYCLE TIME. 6. STEP 6. RETURN TO MAIN MENU. 7. STEP 7. SCREEN "L" = LAST REPORT. 8. STEP 8. PRESS "F" 6 = PRINT OUT. ( CALIBRATION REPORT.) 9. STEP 9. KEY F3 TO RETURN TO MAIN MENU. XII. "A" SCREEN = ANALYZER DEFINITION. 1. STEP 1. KEY ALT + KEY EDIT 2. STEP 2. TAB BAR TO CALIBRATION STREAM 3. STEP 3. CHANGE TO ZERO. 4. STEP 4. KEY F2 SAVE. 5. STEP 5. KEY F3 RETURN TO MAIN MENU FILE: Calibration Procedure
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