BMC Evolutionary Biology 2008, 8:89
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1 BMC Evolutionary Biology This Provisional PDF corresponds to the article as it appeared upon acceptance. Fully formatted PDF and full text (HTML) versions will be made available soon. Selection against tandem splice sites affecting structured protein regions BMC Evolutionary Biology 2008, 8:89 doi: / Michael Hiller Karol Szafranski Klaus Huse Rolf Backofen Matthias Platzer ISSN Article type Research article Submission date 27 November 2007 Acceptance date 21 March 2008 Publication date 21 March 2008 Article URL Like all articles in BMC journals, this peer-reviewed article was published immediately upon acceptance. It can be downloaded, printed and distributed freely for any purposes (see copyright notice below). Articles in BMC journals are listed in PubMed and archived at PubMed Central. For information about publishing your research in BMC journals or any BioMed Central journal, go to Hiller et al., licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License ( which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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22 A tandem donor % introns UTR CDS order disorder B tandem acceptor 4 % introns Figure 1 0 order disorder
23 control intron tandem donor tandem acceptor * helix sheet non regular ** inside outside accessibility 5 0 *** 1 hydropathy * Figure ***
24 % introns Figure 3 B C % introns CDS UTR CDS UTR Human C. elegans % introns order disorder Human plausible NAGNAG implausible NAGNAG
25 order expected disorder plausible implausible human specific human chimp specific human, mouse, dog, chicken conserved Figure 4
26 % eliminated mutations A Figure 5 exonic synonymous mutations *** 2.6 CDS 1.1 UTR *** 3.3 order mouse NAGNAH NAGNBG *** 2.0 disorder *** 1.6 NAGNAG CDS 1.4 UTR C. remanei B intronic mutations *** 4.3 CDS 2.3 UTR NAHNAG NBGNAG *** 6.4 order mouse NAGNAG * 2.9 disorder
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