ATP (µmol/gm wet wt.) Meyer, RA, GA Dudley & RL Terjung. J. Appl. Physiol. 49: , 1980.

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6 "& Relaxation rate constant Relative 1/τ (s -1 ) (at 37 C, using G ATP = -32.8kJ/mol) Relaxation rate constant 1/τ (s -1 ) C G ATP G ATP Dawson MJ, DG Gadian & DR Wilkie. J Physiol 299:465-84, 1980.EC KI?AHA=N=JEH=JA?IJ=J=I=BK?JEBJDAAAHCO=L=E=>ABH)62 / )62 )@=FJA@BH,=MIAJ=#MEJDFAHEIIE6DAHECE=@=J=>J=EA@=J"+IAAEIAHJ D=LA>AAHA?=?K=JA@JL=KAIANFA?JA@=J!%+=EF=A

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8 " (µmol/min/g wet wt) Initial Rate of ADP Accumulation AK1 -/- WT ADP accumulation 0.87±0.08 µmol/g (n=20) ~1.5 mm 0.10±0.05 (17) Tetanic contraction frequency (min -1 ) Hancock, CR, et.al. Am. J. Physiol. 288:C1287-C1297, 2005.EC! 6DAEEJE=H=JAB ),2=??KK=JEE =@AO=JA?KJ) =@ME@JOFAE?A)@=FJA@ BH0=??AJ=$ MEJDFAHEIIE Fatigue Contraction Characteristics Percent of Initial Tension tetani/min WT AK1 -/- 1st 40th AK1 -/- WT Contraction Number Milliseconds Hancock, CR, et.al. Am. J. Physiol. 288: C1298-C1304, EC" IIBKI?ABH?A@KHECEJAIA?JH=?JE?@EJEI JAJ=EEABJF=A=@=?F=HEIBJDA?JH=?JEBH?AFHBEA>AJMAAJDABEHIJ=@"JD?JH=?JEEJDAIAGKA?ABH =@AO=JA?KJ) =@ME@JOFAE?A6DA"JD?JH=?JEM=IIAA?JA@IE?AKI?A ),2M=IAAL=JA@J#@KHECJDEIJEA)@=FJA@BH0=??AJ=$MEJDFAHEIIE

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

app AMP ATP H K free = ATP ATP free free GATP

app AMP ATP H K free = ATP ATP free free GATP 74).20;51/;),20)4)+/; %#&"&!`&' MMMFFH=MF )), 271951! )2)46*795) /4),;5 )-4+) *4-1-951 " ))5151 # 205204;)6126-61)160-,1)6-/159-4 ),),2.4-- 1501/0-41+).75+-5)64-56 1-,74)+-)60-6-560)152416-45 ),1764)1-,57*-+65,AF=HJAJB2DOIECO=@*E?DAEIJHO7ELAHIEJO5?DB2DOIE?=-@K?=JE+H=?M