modencode Galaxy: Uniform ChIP-Seq Processing Tools for modencode and ENCODE Data
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1 modencode Galaxy: Uniform ChIP-Seq Processing Tools for modencode and ENCODE Data Quang M Trinh Ontario Institute for Cancer Research qtrinh@oicr.on.ca
2 Outline Model Organism ENCyclopedia Of DNA Elements ( modencode ) project & mandates for the modencode Data Coordinating Center ( DCC ) modencode data & Galaxy on Amazon Cloud Uniform Processing/Peak calling pipeline for modencode & ENCODE (ENCyclopedia Of DNA Elements ) data using Galaxy 2
3 Model Organism ENCyclopedia Of DNA Elements ( modencode ) Project Funding by the National Institutes of Healths (NIH) Aim of modencode is to provide a comprehensive encyclopedia of functional genomics for both worm and fly 11 groups of data providers 1 analysis center 1 Data Coordinating Center ( DCC ) 3
4 Mandates for the DCC Collect, validate, and release data submitted from the 11 groups of data providers Collection Data upload via a website or an ftp site Validation uses controlled vocabularies to describe data and metadata QC to ensure consistency and completeness of submission Integrates data Release Over 10 TB of data publicly available on faceted browser, modmine, and clouds ( Amazon & Bionimbus ) 4
5 modencode Data on Amazon Cloud The entire set of modencode data is on Amazon Cloud as a list of snapshots Custom modencode Amazon Machine Image (AMI) with the entire data pre-mounted for convenience. Users can also select and mount any of the snapshots automated Step-by-step instructions on how to use the custom AMI or how to mount modencode data snapshots 5
6 Main Challenges With Accessing the Entire modencode Data Set Downloading the entire data set ( over 10TB ) from Amazon Cloud will take a while Additional local disks & computing resources are needed Tools for analysis Setup tools locally will also take a while 6
7 Our Solution: modencode Galaxy on Amazon Cloud Bring tools and analysis to our data on Amazon Cloud Build and integrate tools and workflows to Galaxy on Amazon Cloud Automate Galaxy launching on Amazon Cloud and installations of modencode tools on Galaxy and Galaxy cluster 7
8 modencode Galaxy on Amazon Cloud Put together by our co-op students Ravpreet Setia, Fei-Yang (Arthur) Jan, Ziru Zhou, Karming Chu Scripts to launch Galaxy and install tools and their dependencies Peak calling and QC tools SPP, macs2, peak ranger, and bamedit Workflows Uniform processing/peak calling pipeline for modencode and ENCODE data Worm, fly, human, and mouse Enable users to import modencode data directly from the faceted browser to Galaxy Step-by-step documentations 8
9 Simple Steps to Launch modencode Galaxy & Installations of Tools Setup Amazon credentials and environments ( one time ) Setup Galaxy config.txt ( one time ) Launch Galaxy on Amazon Cloud bin/modencode_galaxy_create.pl config.txt Setup Galaxy Cluster using CloudMan console Setup modencode tools for Galaxy Install tools in parallel using bin/auto_install.pl 9
10 Setup Amazon Credentials and Environments ( env.sh ) 10
11 Setup Configurations ( config.txt ) New Galaxy AMI is available on June 29 see from Enis Afgan to galaxy- dev 11
12 12
13 13
14 Uniform Processing/Peak Calling Pipeline A uniform pipeline for calling peaks and ranking reproducibility between replicates for ChIP-seq data Used by both modencode and ENCODE communities for human, mouse, worm, and fly Begins with raw FASTQ files and ends with peak files in BED format and pdf plots of consistency comparisons between replicates. 14
15 Uniform Processing/Peak Calling Pipeline for 3 replicates Control Rep1 Groomer BWA Control Rep2 Groomer BWA merge ControlRep0 Control Rep3 Groomer BWA ChIP Rep1 Groomer BWA ChIP Rep1 ChIP Rep2 Groomer BWA ChIP Rep2 ChIP Rep3 Groomer BWA ChIP Rep3 15
16 Uniform Processing/Peak Calling Pipeline for 3 replicates ( cont d ) ControlRep0 ChIP Rep1 ChIPRep1_VS_ControlRep0 MACS2 ChIPRep2_VS_ControlRep0 IDR IDR- Plot ChIPRep1 VS ChIPRep2 ChIP Rep2 MACS2 IDR IDR- Plot ChIPRep1 VS ChIPRep3 ChIP Rep3 MACS2 ChIPRep3_VS_ControlRep0 IDR IDR- Plot ChIPRep2 VS ChIPRep3 16
17 Uniform Processing/Peak Calling Workflows Galaxy/tree/master/workflows 3-replicate and 2-replicate workflows 17
18 Conclusions Galaxy is a great platform for data analysis We chose Galaxy because of its availability, functionality, and ease of result reproducibility Integrated modencode tools & workflows with Galaxy on Amazon Cloud Works great with the entire modencode data set on Amazon Cloud For more info, see 18
19 Acknowledgments Co-op students Rav Setia Fei-Yang ( Arthur ) Jen Ziru Zhou Karming Chu modencode DCC Data Wranglers Marc Perry Ellen Kephart Sergio Contrino Peter Ruzanov Lincoln Stein ( PI ) 19
20 Funding provided by 20
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