SI8000 Cell Motion Imaging System
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1 SI8000 Cell Motion Imaging System New Product Award Sony Biotechnology Inc.
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3 SI8000 Cell Motion Imaging System The Sony SI8000 Cell Motion System uses high speed video microscopy to detect and record cell motion. A unique motion vector analysis algorithm quantifies cell motion. This technology permits monitoring of non-labeled cells over time in their physiological environment so that cell motion can be analyzed in a non- invasive manner. The system leverages Sony expertise and patented imaging technologies for the ultimate sensitivity, speed, and resolution of cell motion. In addition, the SI8000 uses vector analysis originally developed for video decoding to analyze cell movement. The Sony SI8000 won the 2015 outstanding new product award from the Science and Laboratory Automation and Screening (SLAS) organization. High speed video recording, to track cell motion Label free motion analysis High throughput User-friendly software for quantification and visualization
4 High Speed Motion Capture, Quantification and Visualization The Sony SI8000 system is equipped with a phase contrast microscope, a high-speed camera, CO2-incubator and specialized software. The system automatically detects and adjusts image focus and light conditions. Video imaging captures activity at a high frame rate (150 fps) to deliver data rich results for analysis. Motion vector analysis, based on video analysis technologies developed by Sony for its audio-visual products now allows researchers to analyze movement of cells over a period of time. This permits the system to be effective for analysis of single cells, cell membrane, a single monolayer of cells or for cell-to-cell interaction and intracellular activity. Phase-contrast Microscope Stage Top Incubator (option) Fluorescence Observation Unit (option) Video Camera Image acquisition Motion vector analysis Previous frame Search area Current frame Videos are recorded at a high frame rate Acquisition software automatically detects and adjusts image focus based on light conditions High spatial-temporal resolution enables the detection of motion vectors in cell images (red arrow). 4
5 The SI8000 is label-free so that cells may be observed in their natural state ensuring that there are no outside interactions from potentially toxic dyes or fluorochromes in the environment. This capability is particularly useful for toxicity studies as it allows researchers to see how cells behave as a drug is added to the system. An optional CO2 incubator reliably manages an optimal environmental for in-vitro cell observation. The scanning stage controls the X, Y and Z position of the imaging system for precise and repeatable positioning. Data analysis software lets researchers quantify and visualize cells in motion. A variety of contractile parameters are supported by quantification including contraction and relaxation speed, average deformation distance as well as contraction and relaxation duration. Power Spectrum Density, that measures signal strength is also supported. Graphic outputs include heat maps of contraction, relaxation, propagation and isochrone maps used to detect abnormalities in cardiac cells. Cellular and sub-cellular motion visualization is also supported. Quantification Quantification capabilities includes contraction speed, relaxation speed, average deformation distance, contraction duration, and relaxation duration. Power Spectrum Density measures signal strength. Visualization Visualizations include heat maps of contraction and relaxation, propagation of contraction and isochrone maps used in cardiology to visually detect abnormalities. Cellular and sub-cellular motion visualization is also supported. PDS LOW H Speed H L L 5
6 Cardio Model Applications Contractile motion of single cardiomyocyte derived from hips cell (hips-cm) Example of a motion waveform representing contraction and relaxation peaks, calculated with the single human ipsderived cardiomyocyte (hips-cm) correspond to the same time points. The train of motion waveform was calculated with the single hips-cm. Phase contrast Vector Motion speed 30 1 [µm/s] Average speed [μm/s] Average speed [μm/s] Time[s] Time[ms] Contraction Relaxation 6
7 Label-free visualization of contraction propagation in cardiomyocyte monolayer Magnitude of all motion vectors detected for the whole image ( pixels) is shown with a heat map. Upper panels represent contraction wave and lower panels are relaxation wave. The interval of each picture =13ms. Field of view: 4mm 4mm. Contraction wave 2mm Relaxation wave 2mm 0ms 13ms Propagation of contraction 26ms 39ms 52ms 65ms 78ms 91ms 104ms Isochrone map 7
8 Research Model Applications Cell viability assay with human ips cell derived neural cell Extraction of low frequency, (PSDLOW) component of subcellular motion. A. Visualization of subcellular motion speed of human ips cell derived neural cell (CDI). B. Visualization of PSDLOW. C. Apoptotic cells after 17 hours (yellow arrow). A B C Power spectrum analysis 1 High Speed H H PDS LOW Frequency[Hz] Power Spectrum Density(PSD) L L 0 Low 0 Time[s] 215 8
9 Migration Assay with human cancer cell A. Macro and micro motion of neural cells. B. Color maps of macro and micro motion of HT1080 fibrosarcoma cell line. C. Quantification of migration area in HeLa cervical cancer cells. A Macro (cellular) motion phase contrast (Control) 60min 180min 300min Macro (cellular) motion Micro (subcellular) motion B Directed Motion Micro (subcellular) motion (5 fps) 60min 180min 300min High High color map Speed Low Low C Migration area (Cell Area inside Blue Square) 60min 180min 300min 60min 180min 240min cell area Vector (Psudopodia motion) Phase contrast Migration distance [μm] Migration Area Control Paclitaxel 10nM GM µM Cytochalasin D 0.2µM Time [min] 9
10 Specifications Imaging Unit Cardio model (SI8000C) Research model (SI8000R) Microscope 4x, 10x, 20x Phase-contrast Microscope (auto-focus) Light White-color LED Video Camera Frame Rate: fps Pixels: max x 2048 pixels Frame Rate: 1-100fps (variable by videorate) Pixels: max x 2200 pixels Required environment Temperature: 5-35 C change : ±5 C) Humidity: 20-80%RH (no condensation) Avoid direct sunlight Work Station Hi-Performance GPU 30 Wide-Monitor (2560 x 1600 WQ x GA) SI8000C View Software SI8000R View Software Software Microscope & Video Control SI8000C Analyzer Software SI8000R Analyzer Software Data Analysis for Cardio model Data Analysis for Research model Data Format Video: AVI/Still: BMP/Graph: PNG/Analysis Data: CSV or Open XML 10
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12 North America/International Japan Europe 1730 North First Street San Jose, CA U.S.A. Voice: FAX: , Konan, Minato-Ku, Tokyo, Japan Tel: Fax: The Heights, Brooklands. Weybridge, Surrey, KT13 0XW, UK Tel: +44 (0) Sony Biotechnology Inc. All rights reserved. Sony, the Sony logo, Sony Biotechnology Inc. and logo, are trademarks of Sony Corporation. For Research Use Only. Not for use in diagnostic or therapeutic procedures. Specifications subject to change without notice
North America/International Japan Europe
North America/International Japan Europe 1730 North First Street San Jose, CA 95112 U.S.A. Voice: +1 800-275-5963 FAX: +1 408-352-4130 sales@sonybiotechnology.com http://www.sonybiotechnology.com 1-7-1,
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