Becker & Hickl GmbH. Technology Leader in Photon Counting. Product Catalog

Transcription

1 Becker & Hickl GmbH Technology Leader in Photon Counting Product Catalog 2017

2 About bh Technology Leader in Photon Counting Founded in 1993, Becker & Hickl have introduced a proprietary time-correlated single-photon counting principle that made TCSPC more than 100 times faster than the existing devices. Moreover, bh introduced a multi-dimensional TCSPC process that records the photons not only versus the time in the signal period, but also versus other parameters, such as experiment time, wavelength, or spatial coordinates. The bh devices are designed to record multi-dimensional photon distributions, time-resolved images, sequences of photon distributions, or multidimensional time-tag data. The bh TCSPC products are complemented by bh picosecond diode lasers, detector modules, multi-spectral detector assemblies, and experiment control modules. Based on these components Becker & Hickl supply their own confocal fluorescence lifetime laser scanning microscope and FLIM upgrade kits for laser scanning microscopes of various manufacturers. Moreover, bh is supplier of TCSPC modules for commercial time-domain optical tomography instruments. bh market activities include currently five workshops around the world yearly and the distribution of more than 1500 pages of TCSPC literature Introduction of multidimensional TCSPC. Introduction of fast TAC/ADC conversion. TCSPC becomes 100 times faster than it was before. SPC- 300 modules with 32 or 128 simultaneously recording detector channels. Time channel width 820 fs. Saturated count rate 3 MHz SPC-330 modules. Improved IRF width. Routers for parallel operation of detectors SPC-430 modules. Continuous Flow mode for infinite sequential recording. Saturated count rate 8 MHz. First single-molecule applications. Resolution of bursts, lifetime analysis within bursts SPC-432 modules. Introduction of Time-Tag recording for single molecule spectroscopy. First version of PML-16 sixteen channel PMT module SPC-535 modules. First implementation of TCSPC FLIM. First FLIM applications. PMS-300 gated photon counter and multichannel scaler 1997 SPC-630 modules. Introduction of PCI bus. Modules contain time-tag mode, standard photon distribution modes, and continuous flow mode. PMH-100 detector module simplifies system setup SPC-730 modules. Introduction of TCSPC FLIM. Scan Sync In and Scan Sync out mode. First applications in FLIM microscopy. MSA-300 multichannel scaler. 100 MHz count rate, 5 ns channel width SPC-134 four-channel packages for optical tomography. Devices contain continuous flow mode, standard photon distribution modes, and time-tag mode. Saturated sustained count rate 32 MHz. SPC-130 TCSPC board FLIM upgrade kits for Zeiss LSM 510 NLO microscopes. SPCImage FLIM data analysis software. MSA-1000 multichannel scaler. 1 ns resolution, count rate 1000 MHz. BHL-600 red and NIR picosecond diode lasers. New SPCM software introduces autosave, auto-accumulate, and automatic page stepping functions 2001 SPC-830 modules. Fast DMA bus interface. Saturated sustained count rate 8 MHz. Standard photon distribution modes, FLIM modes, time-tag recording. For the first time, FLIM and single-molecule techniques were combined in one instrument. DCC-100 detector controller solves the problem of detector overload. PMC-100 cooled detector modules. Internal preamplifier, internal high-voltage generator, overload shutdown. System setup is greatly simplified. Detector-Shutter modules for non-descanned ports of multiphoton microscopes FLIM systems for Leica, Olympus, and Biorad laser scanning microscopes. First multi-spectral FLIM. BHLP-700 NIR picosecond diode lasers. Up to 10 mw at 785 nm. BDL-405 UV picosecond diode lasers. DDG-100 pulse generator for multiplexed operation of lasers and SPC modules. First demonstration of double-exponential FRET imaging SPC-144 four-channel TCSPC Imaging Package. Saturated sustained count rate 40 MHz. BDL-375, 440, and 473 picosecond diode lasers. PML-16 multi-wavelength detector. Online FCS, PCH, and MCS trace calculation in the time-tag mode of the bh TCSPC modules 2004 Simple-Tau 140 and Simple-Tau 830 compact TCSPC systems. MW-FLIM, first multi-spectral NDD FLIM systems for multiphoton microscopes. Macro-time synchronisation of several TCSPC modules. First full correlation down to the picosecond region New PML-16 sixteen channel detector. Internal high-voltage generator, overload shutdown, control via DCC-100. High-efficiency single-mode fibre coupling of BDL picosecond diode lasers. FLIM upgrade kits for confocal laser scanning microscopes based on bh BDL-SM lasers and bh TCSPC technology. W. Becker, Advanced Time-Correlated Single Photon Counting Techniques published by Springer. First edition of BH TCSPC Handbook. 360 pages, 364 references High-power CW mode for BDL-SMC picosecond diode lasers. Second edition of bh TCSPC Handbook. 387 pages, 387 references DCS-120 confocal laser scanning systems. Complete fluorescence-lifetime laser scanning microscopes. DPC channel photon correlator, 160 ps resolution. SPC-154 package and SPC-150 modules. Standard photon distribution modes, continuous flow mode, TCSPC imaging, continuous-flow imaging, imaging in the parameter-tag mode for SPC-150 and SPC FLIM systems for Zeiss LSM 710 microscopes. NDD FLIM systems for Leica SP2 MP and SP5 MP microscopes. Simple-Tau 152 and -154 two and four channel TCSPC systems. 3rd edition of bh TCSPC Handbook GaAsP hybrid detectors. 8-channel parallel TCSPC and TCSPC FLIM systems 2010 Eight-channel SPAD detector. GaAsP hybrid detectors in all bh FLIM systems. Z stack FLIM. 4th edition of bh TCSPC Handbook Phosphorescence Lifetime imaging (PLIM) in DCS-120 and Zeiss LSM 710 FLIM systems. Fluorescence Lifetime-Transient Scanning (FLITS) DCS-120 Wideband, DCS-120 Multiphoton, and DCS-120 Macro FLIM systems 2012 DCS-120 electronic alignment, NIR FLIM, FLITS integrated in DCS-120 and LSM 710 FLIM systems, 5th edition of bh TCSPC Handbook MW-FLIM and PML-16 GaAsP multi-wavelength detectors, SPC-150N TCSPC module, BDL-SMN series and BDS series picosecond diode lasers. Single-molecule burst analysis software bit SPCM software. Megapixel FLIM, Mosaic FLIM, triggered accumulation of time series by mosaic FLIM, Z stack recording by Mosaic FLIM. SPC-160 TCSPC modules. 6th edition of bh TCSPC Handbook Imaging of Ca 2+ transients in neurons. SPC-150NX module, world record in TCSPC time resolution with superconducting NbN detector. Fibrebased TCSPC systems for in vivo applications. W. Becker (ed.), Advanced Time-Correlated Single Photon Counting Applications published by Springer Fast Online FLIM, FLIM with Abberior STED microscopes, SPC-106pcie, DCC-100pcie, GVD-120pcie PCI-Express TCSPC, Detector Control, and Scan Control modules. Simple Tau II TCSPC systems with Thunderbolt interface. FLIM for Sutter Instrument MOM microscopes. 2

3 More than 1600 TCSPC Systems Worldwide bh Modular TCSPC Systems - Unsurpassed in Time resolution SPC-160: High performance in all TCSPC applications Internal histogramming modes and photon stream (parameter tag) modes Multi-detector / multi-wavelength / laser multiplexing operation Triggered multichannel scaler (phosphorescence) mode High-speed FLIM / PLIM / FLITS for laser scanning microscopes Megapixel Technology: Mosaic, Time Series, Z-stack, Multi-spectral FLIM High-speed parallel imaging channel FCS / FCCS in combination with fluorescence lifetime Single-molecule multi-parameter burst analysis Unlimited fast sequential recording for fnirs / DOT systems Dead time 80 ns, saturated count rate 12.5 MHz Ultra-fast discriminators, 5 GHz input bandwidth Extra-low low-frequency timing noise Part of bh modular FLIM systems New: SPC-160pcie TCSPC module with PCI Express Interface Functions and parameters see SPC-160 Part of Simple-Tau II system SPC-150: High-end performance at reasonable cost Internal histogramming modes and Photon stream (parameter tag) modes Multi-detector / multi-wavelength / laser multiplexing operation Triggered Multichannel Scaler (phosphorescence) mode High-speed FLIM / PLIM / FLITS for laser scanning microscopes Megapixel Technology: Mosaic, Time Series, Z-stack, Multi-spectral FLIM FCS / FCCS in combination with fluorescence lifetime Single-molecule multi-parameter burst analysis Unlimited fast sequential recording for DOT systems Electrical response 6.8 ps FWHM, electrical timing jitter 3 ps RMS Dead time 100 ns, saturated count rate 10 MHz Expandable with up to 8 detectors or 16-channel multispectral detection Part of bh modular FLIM systems SPC-150N: Improved Timing for Fast Detectors Ultra-fast discriminators, 5 GHz input bandwidth Extra-low low-frequency timing noise Electrical time resolution 6.8 ps FWHM, electrical timing jitter 3 ps RMS Minimum time channel width 813 fs Expandable with up to 8 detectors or 16-channel multispectral detection SPC-150NX: Ultra-Fast Timing for Ultra-Fast Detectors Ultra-fast discriminators, 5 GHz input bandwidth Extra-low timing noise Electrical response 3.5 ps FWHM, electrical timing jitter 1.5 ps RMS Minimum time channel width 406 fs World record in TCSPC time resolution: 17.8 ps FWHM / 7.9 ps RMS with superconducting detector SPC-130 low cost TCSPC Modules Standard fluorescence lifetime, NIRS / DOT, single-molecule spectroscopy Rugged design Overvoltage-protected inputs Photon distribution, time-tag, sequential-recording modes Low price, especially at large order volumes SPC-130-EM TCSPC Modules Improved SPC-130 with fast bus interface and larger memory Standard fluorescence and phosphorescence lifetime, diffuse optical tomography, single-molecule spectroscopy, FCS, photon correlation Rugged design Photon distribution, parameter-tag, sequential-recording modes Also available as SPC-134EM four-channel package 3

4 Technology Leader in Photon Counting SPC-830 TCSPC module Internal histogramming modes and photon stream (parameter tag) modes Multi-detector / multi-wavelength / laser multiplexing operation Triggered MCS (phosphorescence) mode Synchronisation of modules for photon correlation High-speed FLIM / PLIM / FLITS for laser scanning microscopes Megapixel Technology: Mosaic, Time Series, Z-stack, Multi-spectral FLIM FCS in combination with fluorescence lifetime Single-molecule multi-parameter burst analysis Total recordable count rate 4 MHz, saturated count rate 8 MHz Time channel width down to 813 fs, low low-frequency timing noise SPC-154 and SPC-154N Four-Channel TCSPC package Four fully parallel TCSPC / FLIM channels Multi-dimensional photon distribution mode Multi-detector / multi-wavelength operation / laser multiplexing operation Photon stream (parameter tag) mode; buffer size 2 M photons Triggered MCS (phosphorescence) mode Synchronisation of channels for photon correlation High-speed FLIM / PLIM / FLITS for laser scanning microscopes FCS in combination with fluorescence lifetime Single-molecule multi-parameter burst analysis Unlimited fast sequential recording for fnirs / DOT systems Total recordable count rate 20 MHz, saturated count rate 40 MHz Each channel expandable for up to 8 detectors SPC-134 and SPC-134-EM Four-Channel TCSPC packages Four fully parallel TCSPC channels Multi-dimensional photon distribution mode Photon stream (parameter tag) mode; buffer size 8 M photons Synchronisation of channels for photon correlation FCS in combination with fluorescence lifetime Burst-integrated fluorescence lifetime detection Unlimited fast sequential recording: TCSPC module for DOT systems Fast multiplexing of excitation sources Total recordable count rate 20 MHz Simple-Tau Compact TCSPC and TCSPC-FLIM Systems New: Simple-Tau II TCSPC/FLIM systems Thunderbolt interface to system computer One or two SPC-160pcie TCSPC/FLIM modules One DCC-100pcie detector controller Simple-Tau 150, 150N, and 160 compact TCSPC systems One SPC-150, 150N, or 160 TCSPC channel One DCC-100 detector controller Portable DOT systems High-Speed FLIM systems Compatible with bh multispectral FLIM detectors Part of bh DCS-120 confocal scanning FLIM systems Part of bh FLIM systems for Zeiss LSM 510 and LSM 710 family Part of bh NDD FLIM systems for Leica SP2 and SP5 MP Can be upgraded with additional SPC-150 or measurement control cards Simple-Tau 152, 152N, and 162 compact TCSPC systems Two parallel SPC-150, 150N, or 160 TCSPC channels One DCC-100 detector controller Portable DOT systems High-Speed FLIM systems Part of bh DCS-120 confocal scanning FLIM systems Part of bh FLIM systems for Zeiss LSM 510 and LSM 710 family Can be upgraded with additional SPC-150 or measurement control cards Simple-Tau 154, 154N, and 164 compact TCSPC systems Four parallel SPC-150, 150N, or 160 TCSPC channels Portable DOT systems Ultra-High-Speed parallel multispectral FLIM systems Simple-Tau 130 compact TCSPC systems Simple-Tau 130 EM compact TCSPC systems One SPC-130 or SPC-130 EM TCSPC channel One DCC-100 detector controller Standard fluorescence lifetime applications Anti-bunching measurements Can be upgraded with additional SPC-130 or measurement control cards 4

5 More than 1600 TCSPC Systems Worldwide Simple-Tau Big Versions Up to seven TCSPC and measurement control cards User-specific hardware and software configuration High speed parallel FLIM systems Portable DOT systems Simple-Tau Large Screen Versions Combines large screen area with small size of TCSPC system FLIM and other TCSPC Imaging applications High-resolution FLIM in combination with 64 bit SPCM software Multi-Tau 8-Channel TCSPC systems Eight SPC-150 or SPC-130 TCSPC modules controlled from a standard Pentium PC Portable DOT systems Ultra-High-Speed FLIM systems Power-Tau TCSPC systems Up to six TCSPC or measurement control cards in high performance PC Highly modular systems User-specific hardware and software configuration Large system memory for megapixel FLIM applications and DOT High data transfer rate High on-line computation power Picosecond Photon Correlators DPC Channel Picosecond Photon Correlator Recording of absolute photon times in 16 channels Fluorescence correlation down to ps times FCS combined with fluorescence decay 3-channel reversed-start-stop TCSPC mode for PMT inputs 15-channel reversed start-stop mode for LVTTL SPAD inputs 15-channel multiscaler mode TCSPC FLIM Mode Multiscaler FLIM mode Autocorrelation / Cross correlation within 16 LVTTL or 4 CFD channels Simple-Tau DPC System Lap-top based compact system DPC Channel Picosecond Photon Correlator Optional: DCC-100 detector controller card GVD-120 scan controller card 5

6 Technology Leader in Photon Counting Multichannel Scalers PMS-400 Gated Photon Counter and Multichannel Scaler Dated detection of optical signals Luminescence decay in the µs and ms range Chemoluminescence Two parallel recording channels Gating down to 1 ns Multiscaler operation down to 300 ns per channel MSA-300 Multichannel Scaler Recording of luminescence decay functions in the ns, and µs range Time-of-flight mass spectroscopy Lidar Time resolution 5 ns per time channel Maximum count rate 100 MHz Compact Simple-Tau MSA systems available Simple-Tau MSA systems Lap-top based compact MSA-300 systems 1,2, or 3 MSA-300 modules DCC-100 detector controller Recording of luminescence decay functions in the ns, and µs range Time-of-flight mass spectroscopy Lidar SPC-130EM, SPC-150, SPC-160, SPC-830 TCSPC Systems All SPC-130EM, SPC-150, SPC-160, SPC-160pcie and SPC-830 TCSPC Systems have a multichannel-scaler function implemented. The MCS function works simultaneously with the TCSPC process. Applications: Recording of photon bursts from single molecules Single-molecule spectroscopy FCS Simultaneous phosphorescence and fluorescence decay recording Simultaneous FLIM and PLIM Please see bh TCSPC Handbook, available on 6

7 More than 1600 TCSPC Systems Worldwide Detectors and Detector Assemblies bh guarantee that their TCSPC devices work with any photon counting detector HPM and -50 hybrid detector modules Based on Hamamatsu R10467 hybrid detector tubes GaAsP versions: 40% detection efficiency throughout visible spectrum GaAs versions: 15% detection efficiency up to 850 nm GaAsP versions: 130 ps IRF width No afterpulsing Internal high-voltage generator, controlled via bh DCC-100 Active area 3 mm or 5 mm diameter C-Mount adapter Adapter to bh DCS-120 confocal scanning FLIM system Adapter to NDD and BIG port of Zeiss LSM 710 NLO microscopes Adapter to RLD port of Leica SP2 MP and SP5 MP microscopes SMA and FC multi-mode fibre adapters HPM C and -50C cooled hybrid detector modules Based on Hamamatsu R10467 hybrid detector tubes GaAsP versions: 40% detection efficiency throughout visible spectrum GaAs versions: 15% detection efficiency up to 850 nm GaAsP versions: 130 ps IRF width No afterpulsing Reduced dark count rate by cooling Internal high-voltage generator, power supply and control via bh DCC-100 Active area 3 mm and 5 mm diameter C-Mount adapter Adapter to bh DCS-120 confocal scanning FLIM system Adapter to BIG port of Zeiss LSM 710 NLO microscopes Adapter to RLD port of Leica SP2 MP and SP5 MP microscopes SMA and FC multi-mode fibre adapters HPM and -06C ultra-fast hybrid detector modules Based on Hamamatsu R10467 hybrid detector tubes Bi-alkali cathode, 300 to 600nm Clean TCSPC response, no tails and bumps 38ps IRF width No afterpulsing Cooled version: HPM C 100 ma/w PMC-100 Cathode Radiant Sensitivity nm PMC-100 cooled PMT modules Cathode versions for UV to NIR region Internal PMT voltage generation IRF width 150 ps Ultra-stable IRF up to recorded count rates of 5 MHz Internal preamplifier Overload shutdown Power supply and control via DCC-100 detector controller Adapters for Zeiss LSM NDD ports, Zeiss LSM confocal ports, Leica SP2 and SP5 RLD Ports, bh DCS-120 confocal FLIM system Part of the bh modular FLIM and modular DOT systems PMZ-100 PMT modules for FLIM Systems of Zeiss LSM 710 / 780 / 880 Family Microscopes Adapter for Confocal (DC) port Adapter for non-descanned detection (NDD) port Internal PMT voltage generation IRF width 150 ps Ultra-stable IRF up to recorded count rates of 5 MHz Internal preamplifier Overload shutdown Power supply and control via DCC-100 detector controller 7

8 Technology Leader in Photon Counting PML-16 C 16-Channel TCSPC detectors 16-channel detector with routing electronics and internal HV power supply Based on Hamamatsu R5900-L16 multi-anode PMT Simultaneous detection in 16 channels Connects directly to all bh SPC modules Power supply and control via DCC-100 detector controller IRF width 150 to 200 ps Part of bh multispectral TCSPC FLIM detectors PML-16 GaAsP 16-Channel TCSPC detectors 16-channel detector with high-sensitivity GaAsP cathode Based on Hamamatsu GaAsP multi-anode PMT Simultaneous detection in 16 channels Internal Routing electronics and HV power supply Connects directly to all bh SPC modules Power supply and control via DCC-100 detector controller IRF width 150 to 200 ps Part of bh multispectral TCSPC FLIM detectors Single-photon avalanche photodiode modules (SPADs) Manufacturer: id Quantique Direct-coupled and fibre-coupled versions Active area d=20µm and d=50µm Ultra-high efficiency in visible region IRF width 40 ps Active areas of 20µm, 50µm Stable IRF over count rate Dark count rates <5 s -1 (id ULN) and <20 s -1 (id ULN) Detector count rates up to 8 MHz, recorded count rates up to 5 MHz Direct-coupled and fibre-coupled versions Focusing and alignment adapters id-220 InGaAs IR SPAD Detectors Manufacturer: ID Quantique Detection from 900nm to 1700nm Quantum efficiency up to 20% Continuous operation - no gating required Compatible with all bh TCSPC devices IRF width 220 ps FWHM Fibre coupling, FC connector Fluorescence and phosphorescence decay recording Fluorescence correlation FLIM, PLIM New! id-230 InGaAs IR SPAD Detectors Manufacturer: ID Quantique Detection from 900nm to 1700nm Quantum efficiency up to 25% Extremely low dark count rate - down to 250 counts / s Continuous operation - no gating required Compatible with all bh TCSPC devices IRF width <80 ps FWHM Fibre coupling, FC connector Fluorescence and phosphorescence decay recording Fluorescence correlation FLIM, PLIM New! SCONTEL Superconducting NbN Detectors Manufacturer: SCONTEL, Moscow Detection from 400nm to 1700nm IRF width down to 17.8 ps FWHM with SPC-150NX Timing Jitter with SPC-150NX 7.8 ps rms 8

9 More than 1600 TCSPC Systems Worldwide Multi-Spectral TCSPC / FLIM Detectors PML-SPEC and PML-SPEC GaAsP multi-wavelength detection modules Simultaneous fluorescence decay detection in 16 wavelength channels SMA 905 or FC fibre adapter Fibre diameter up to 1 mm Multi-spectral FLIM for confocal laser scanning microscopes IRF width 200 ps Works with all bh TCSPC modules Internal high-voltage power supply Internal routing electronics Overload shutdown Full control via DCC-100 card Part of the bh multi-wavelength FLIM systems for laser scanning microscopes Adapter for DCS-120 confocal scanning FLIM system MW FLIM multi-spectral FLIM detection modules for multiphoton microscopes Multi-spectral FLIM in 16 wavelength channels Fibre bundle for large area detection and area transformation Non-descanned (NDD) detection or confocal detection Adapters for Zeiss, Leica, Olympus, Nikon, bh DCS-120 IRF width 150 ps FWHM Works with all bh TCSPC modules Internal high-voltage power supply Internal routing electronics Protected by overload shutdown Shutter for input protection available Full control via DCC-100 card Part of the bh multi-wavelength FLIM systems for laser scanning microscopes New! MW FLIM GaAsP multi-spectral NDD FLIM detection systems for multiphoton microscopes Multi-spectral FLIM in 16 wavelength channels High-efficiency GaAsP cathode Fibre bundle for large area detection and area transformation Non-descanned (direct) detection or confocal detection Adapters for Zeiss, Leica, Olympus, bh DCS-120 IRF width 200 ps FWHM Works with all bh TCSPC modules Internal high-voltage power supply Internal routing electronics Protected by overload shutdown Shutter for input protection available Full control via DCC-100 card Part of the bh multi-wavelength FLIM systems for laser scanning microscopes Opto-Mechanical Detector Components Detector / Shutter Assemblies for Various PMTs For NDD ports of multiphoton laser scanning microscopes Shutter operation via DCC-100 detector controller Overload shutdown of PMT Input field lens For bh PMC-100 and HPM-100 modules, Hamamatsu R3809U, Hamamatsu H7422 Part of bh modular FLIM systems Beamsplitter / detector assemblies For HPM-100, PMC-100, R3809U and H7422 detectors Dichroic beamsplitters, polarising beamsplitters Input filter, bandpass filters in front of detectors Compatible with NDD ports of multiphoton laser scanning microscopes Part of bh modular FLIM systems Detectors and Detector Assemblies with Fibre Adapters For HPM-100, PMC-100, R3809U and H7422 detectors Input filter, bandpass filters in front of detectors 9

10 Technology Leader in Photon Counting Detector Electronics DCC-100 detector controller New: DCC-100 pcie detector controller with PCI express interface Power supply for two detectors Power supply of thermoelectric coolers of detectors Power supply of preamplifiers Detector gain control Detector overload shutdown Control of shutters or actuators Control of bh detector / shutter assemblies Intensity control of bh BDL and BDS ps diode lasers Integrated in SPCM TCSPC software package Part of the bh modular FLIM, modular DOT systems Part of bh Simple-Tau TCSPC systems HVM-3000 High-Voltage module for PMTs and MCP-PMTs Small size, attaches directly to PMT or MCP SHV connector, connects to Hamamatsu R3809U MCP PMTs and Photek FGN wide-field TCSPC detector +12 V power supply via bh DCC-100 detector controller Output voltage -400 to V, controlled via DCC-100 detector controller HRT-41 four-channel router Connects up to four PMC-100, H7422 or R3809U detectors to one TCSPC channel All detectors are detecting simultaneously HRT-81 eight-channel router Connects up to eight PMC-100, H7422 or R3809U detectors to one TCSPC channel All detectors are detecting simultaneously HRT-82 eight-channel router Connects up to eight SPAD modules to one TCSPC channel All detectors are detecting simultaneously HFAC-26 Preamplifiers For R3809U MCP PMT, H7422 modules, and other PMTs Power supply from SPC or DCC module Overload shutdown in conjunction with DCC-100 controller Overload thresholds 0.1µA, 0,3µA, 1µA, 2µA, 3µA, 10µA, 100µA HFAH-26 Preamplifiers Low-noise For R3809U MCP PMT, H7422 modules, and other PMTs Power supply from SPC or DCC module Overload shutdown in conjunction with DCC-100 controller HPM-CON-02 Frequency-to-Analog Converter for PMT Pulses Converts single-photon-pulse signals of photon-counting detectors into an intensityproportional analog signal Analog output independent of single-photon pulse amplitude Inserts directly in detector output pulse line Provides photon pulses to TCSPC modules and intensity signal to analog electronics Input pulse amplitude -30 mv to -200 mv, input pulse width down to 500 ps Input pulse rate up to 107 pulses per second Output voltage range 0 to +4.9 V Power supply 5V from bh SPC or DCC module New! APS-100 TCSPC Synchronisation Module For synchronisation of TCSPC experiments with Ti:Sapphire lasers Output amplitude widely independent of laser intensity and laser wavelength Intensity range 1:100 Power supply from SPC or DCC module PHD-400 PIN Photodiode Modules For synchronisation of TCSPC experiments with lasers Power supply from SPC or DCC module Detector area 0.25 mm2 Current indicator for easy alignment 10

11 More than 1600 TCSPC Systems Worldwide Picosecond Diode Lasers BDS-SM series picosecond diode lasers Small-size OEM Module, single-mode output Dimensions 40 mm x 40 mm x 120 mm Wavelengths 375, 405, 440, 473, 488, 510, 640, 685, 785, 1064, 1300 nm Single-Mode free-beam or single-mode fibre output Pulse width down to 50 ps Pulse repetition rate 50 MHz and 20 MHz CW equivalent power up to 5 mw New: CW mode with up to 50 mw power Fast on / off / multiplexing capability Sync input, Sync output All electronics integrated Operation from simple +12 V power supply New! BDS-SMY series green / yellow picosecond diode lasers Small-size OEM Module, single mode output Wavelength 532 nm, 561 nm and 594 nm Pulse width down to 50 ps Pulse repetition rate 50 MHz, CW equivalent power up to 0.7 mw Operation from simple +12 V power supply New! BDS-MM series picosecond diode lasers Small-size OEM Module, multi-mode output Dimensions 40 mm x 40 mm x 120 mm Wavelengths 445, 510, 640, 685, 785, 830, 980, 1064 nm Multi-Mode free-beam or multi-mode fibre output Pulse width down to 90 ps Pulse repetition rate 50 MHz and 20 MHz CW equivalent power up to 25 mw Fast on / off / multiplexing capability Sync input, Sync output All electronics integrated Operation from simple +12 V power supply BDL-SMN Series Picosecond / CW diode lasers Wavelengths 375, 405, 440, 473, 488, 510, 640, 685, 785, 1060 nm Driver and control electronics integrated laser head Power regulation in ps mode and in CW mode Beam corrector removes astigmatism and makes beam circular Interfaces with all commonly used fibre couplers Repetition rate 20, 40, 80 MHz High-Power CW mode Pulse width typically 40 to 80 ps for up to 1 mw at 50 MHz Improved pulse shape at high power, typ. 200ps at 5 mw / 50 MHz Fast on / off / multiplexing capability Part of the bh modular FLIM systems for laser scanning microscopes The BDL-SMN lasers were designed in cooperation with Lasos GmbH, Jena Free-beam versions Highly corrected beam Circular cross-section and low astigmatism Fibre-coupled versions Fibre coupling with up to 70% coupling efficiency Fibres with FC output or with collimated output Part of DCS-120 Confocal Scanning FLIM Systems Fibre-pigtail versions Single-mode fibre pigtail Single-mode fibre permanently attached to laser No fibre alignment required Designed and manufactured in cooperation with Lasos GmbH, Jena, Germany All lasers operate from single +12V power supply All bh laser have the electronics integrated in the laser head No external controller unit Operation from single +12V power supply All Lasers interface directly with DCC-100 detector controller, GVD-100 scan controller, or DCS-120 controller box. 11

12 Technology Leader in Photon Counting Experiment Control and Connecting Electronics DDG-210 Digital Delay Generator Multiplexing of lasers and routing to TCSPC Modules Multiplexing rate up to 1 MHz Laser on/off modulation for PLIM Non-overlapping laser multiplexing On-times programmable individually Part of bh modular DOT systems Part of bh FLIM / PLIM systems for Zeiss LSM 710 / 780 / 880 family Integrated in bh SPCM TCSPC instrument control software GVD-120 Scan Controller Card New: GVD-120 pcie Scan Controller Card Generation of scan signals for galvanometer scanners and piezo stages Dual-axis control Frame scan, line scan, high-stability point operation Laser on/off modulation for PLIM Linear x scan with cycloid flyback New: Sinusoidal x scan option Extremely high scan rates Controls also two bh BDL-SMC, BDL-SMN, or BDS ps diode lasers Laser beam blanking Laser multiplexing, Laser on/off modulation for PLIM Fully digital signal generation Independent of software response times Pixel times down to 0.5 µs Pixel numbers up to 4048x4048 Fully integrated in bh SPCM TCSPC software Part of bh DCS-120 confocal scanning systems DCC-100 and DCC-100pcie detector controllers Control of two bh TCSPC detectors Intensity control of bh picosecond diode lasers For details please see Detector Electronics DB-32 Delay Switch Box 32 step passive delay line Delay from 0 to 31.5 ns in 0.5 ns steps New! Selection of one of two signal sources Transmits any signal waveform Signal bandwidth 1 GHz No noise, no jitter No external power supply needed Settings persist when box is disconnected from USB Easy adjustment of TCSPC SYNC path length Control integrated in bh SPCM TCSPC operating software BOB-104 Signal Distribution Box Distributes scan clock signals to four SPC modules Combines routing signals and other control signals into 15-pin control inputs of four SPC-130, -150, and -160 modules Auxiliary +12V input for power supply of amplifiers, routers, detectors BOB-101 Signal Distribution Box Combines routing signals and other control signals into 15-pin control inputs of one SPC-130, -150, and -160 modules Auxiliary +12V input for power supply of amplifiers, routers, detectors DCS Connection Box Power supply and control of two bh BDL-SMC or BDL-SMN ps diode lasers Connects scan clocks and laser multiplexing signals of GVD-120 scan controller into two SPC-150 or SPC-830 modules and two BDL-SMC or BDL-SMN diode lasers Connects routing signals of two bh multi-spectral FLIM detectors into SPC modules Configurable by SPCM software Cables and Adapters A-PPI-D passive pulse inverters 1:3, 1:4 power splitters and combiners Attenuators SMA cables SMA-to SMA adapters SMA-to BNC adapters Power supply cables for detectors Interface cables for bh FLIM systems for various laser scanning microscopes A reasonable set of cables and adapters is delivered with each bh SPC module or Simple-Tau system 12

13 More than 1600 TCSPC Systems Worldwide Fluorescence Lifetime Microscopy DCS-120 Confocal Scanning FLIM Systems Based on bh s 64-bit megapixel FLIM technology FLIM with up to 2048 x 2048 pixels Complete Confocal Laser Scanning FLIM microscopes FLIM upgrade for existing conventional microscopes Scanning by fast galvanometer mirrors Two fully confocal detection channels One or two BDL-SMC or BDL-SMN picosecond diode lasers Laser wavelengths 375, 405, 440, 473, 488, 510, 640, 685, 785 nm Tuneable excitation by super-continuum laser with AOTF One or two confocal detection channels, parallel acquisition Channel separation by dichroic or polarising beamsplitters Individually selectable pinholes, individually selectable filters GaAsP hybrid detectors for visible range, GaAs hybrid detectors for NIR range 16-channel multi-wavelength GaAsP detector module Z-stack FLIM acquisition with Zeiss Axio Observer Z1 Simultaneous fluorescence and phosphorescence lifetime imaging (PLIM) Fluorescence lifetime-transient scanning (FLITS) Ultrafast time-series recording by temporal mosaic FLIM function Wideband (WB) version, compatible with tuneable lasers Electronic pinhole alignment DCS-120 MP Multiphoton FLIM System Multiphoton version of DCS-120 scanning system Excitation by Ti:Sa laser Laser control integrated in SPCM data acquisition software Laser intensity control and PLIM laser modulation by AOM One or two non-descanned detection channels One or two bh HPM hybrid detectors Clear Images from deep tissue layers Excellent spatial and temporal resolution Images up to 2048 x 2048 pixels, 256 time channels Full field of view of microscope lens scanned Optional 16-channel multi-wavelength GaAsP detector module Z-stack FLIM acquisition with Zeiss Axio Observer Z1 Simultaneous fluorescence and phosphorescence lifetime imaging (PLIM) Fluorescence lifetime-transient scanning (FLITS) Ultrafast time-series recording by temporal mosaic FLIM function DCS-120 Macro System FLIM of macroscopic objects Scan field up to 15 mm diameter FLIM with up to 2048 x 2048 pixels Scanning by fast galvano mirrors Two fully confocal detection channels One or two BDL-SMC or BDL-SMN picosecond diode lasers Laser wavelengths 375, 405, 440, 473, 488, 510, 640, 685, 785 nm Tuneable excitation by super-continuum laser with AOTF One or two confocal detection channels, parallel acquisition Channel separation by dichroic or polarising beamsplitters Individually selectable pinholes, individually selectable filters GaAsP hybrid detectors for visible range, GaAs hybrid detectors for NIR range 16-channel multi-wavelength GaAsP detector module Simultaneous fluorescence and phosphorescence lifetime imaging (PLIM) Fluorescence lifetime-transient scanning (FLITS) Ultrafast time-series recording by temporal mosaic FLIM function Wideband (WB) version, compatible with tuneable lasers Electronic pinhole alignment Please see handbook: DCS-120 Confocal Scanning FLIM Systems, 6th Edition, April 2015 Overview brochure DCS-120 Confocal Scanning Systems FLIM Systems for Zeiss LSM 710 / 780 / 880 Microscopes LSM 710 / 780 / 880 NLO, LSM 7MP Multiphoton Microscopes LSM 710, LSM 780, LSM 880 Confocal Microscopes LSM 710, LSM 780, LSM 880 In Tune systems Based on bh s 64-bit megapixel FLIM technology FLIM with up to 2048 x 2048 pixels Multiphoton FLIM, PLIM, multispectral FLIM, FCS Confocal FLIM, PLIM, multispectral FLIM, FCS FLIM with bh HPM hybrid detectors or Zeiss BIG-2 detectors Fast preview mode, both for intensity and lifetime Mosaic FLIM Z Stack FLIM Fast Time-series FLIM Acquisition by 1, 2, 3 or 4 parallel TCSPC FLIM channels Simultaneous fluorescence and phosphorescence lifetime imaging (PLIM) Fluorescence lifetime-transient scanning (FLITS) fully integrated Ultrafast time-series recording by temporal mosaic FLIM function Confocal NIR FLIM up to 900 nm detection wavelength Two-Photon OPO FLIM up to 900nm detection wavelength Please see handbook Modular FLIM Systems for Zeiss LSM 710 / 780 / 880 Family Laser Scanning Microscopes and Overview brochure 13

14 Technology Leader in Photon Counting Still available: FLIM Systems for Zeiss LSM 510 NLO Multiphoton Microscopes FLIM with up to 2048 x 2048 pixels Multiphoton excitation with non-descanned detection Detectors connected to Zeiss NDD switch box Single-wavelength NDD FLIM Dual-wavelength NDD FLIM Multi-spectral NDD FLIM Fast preview mode Mosaic FLIM Z Stack FLIM Fast time-series FLIM HPM hybrid detectors One or two parallel SPC-150 TCSPC channels PC-based systems or Simple-Tau TCSPC systems Non-Descanned FLIM Systems for Leica SP2 MP, SP5 MP, SP8 MP Microscopes 64-bit megapixel FLIM technology Non-descanned detection via Leica RLD port 1 detector coupled directly to RLD port 2 detectors via external beamsplitter Simple-Tau 150 or 152 TCSPC systems Acquisition in 1 or 2 parallel TCSPC FLIM channels bh HPM hybrid detectors or Leica HYD detectors Multi-spectral FLIM with 16-channel GaAsP detector Works at any scan rate of SP2 and SP5 Fast acquisition, fast preview mode New! Megapixel FLIM, 2048 x 2048 pixels New! Fluorescence lifetime-transient scanning (FLITS) New! Ultra-fast time series by temporal mosaic FLIM New! Simultaneous FLIM / PLIM FLIM Systems for Olympus FV1000 and FV300 Confocals 64-bit megapixel FLIM technology Excitation by bh BDL-405 SMC or BDL-473 SMC picosecond diode laser High efficiency by direct coupling of detectors Single-wavelength detection: PMT, MCP-PMT, or Hybrid PMT Multi-wavelength detection: bh PML-SPEC detector Full overload protection of detectors ROI and Zoom functions of FV1000 or FV300 available Works at any scan rate FCS capability New! Megapixel FLIM New! Fluorescence lifetime-transient scanning (FLITS) Non-descanned FLIM Systems for Olympus FV1000 and FV300 Multiphoton Microscopes 64-bit megapixel FLIM technology Multiphoton FV1000 and FV300 systems with inverted microscopes High efficiency by non-descanned FLIM detection Deep-tissue imaging capability Single-wavelength detection: PMT, MCP-PMT, or Hybrid PMT New! Multi-spectral FLIM with 16-channel GaAsP detector Full overload protection of FLIM detectors ROI and Zoom functions of FV1000 or FV300 available Works at any scan rate New! Megapixel FLIM New! Fluorescence lifetime-transient scanning (FLITS) Non-descanned FLIM Systems for Nikon A1 MP Multiphoton Microscopes 64-bit megapixel FLIM technology One FLIM channel or two parallel FLIM channels High-efficiency PMH-100 hybrid detectors Non-descanned detection for deep-tissue imaging Multi-spectral FLIM with 16-channel GaAsP detector ROI and Zoom functions of A1 available Works at any scan rate Megapixel FLIM Fluorescence lifetime-transient scanning (FLITS) Ultra-fast time series by temporal mosaic FLIM 14

15 More than 1600 TCSPC Systems Worldwide FLIM Systems for Sutter Instrument MOM Microscopes Up to four parallel FLIM channels Multiphoton excitation by Ti:Sa laser Non-descanned detection for deep-tissue imaging Overload protection of FLIM detectors Up to 1024 x 1024 pixels, 1024 time channels High efficiency Fast acquisition SPCM Online FLIM function available Simultaneous FLIM / PLIM PZ-FLIM-110 Stage-Scanning FLIM System Sample scanning by piezo scan stage Excitation by BDL or BDS series ps diode lasers Confocal detection HPM-100 hybrid detector Optional PML-SPEC GaAsP multi-spectral detector Excellent contrast and resolution Fully controlled by bh SPCM TCSPC/FLIM data acquisition software Compact electronics, integrated in bh Simple Tau system Megapixel FLIM technology - images up to 2048 x 2048 pixels Lateral (x-y) an vertical (z) scanning Simultaneous FLIM / PLIM FLIM for NSOM Systems For NSOM systems of Nanonics and NT-MDT Combines atomic-force and fluorescence lifetime information High sensitivity by HPM-100 hybrid detectors Fluorescence and phosphorescence lifetime imaging Single-point transient-lifetime recording Please see bh TCSPC Handbook or contact bh. FLIM Systems for Clinical Imaging 64-bit megapixel FLIM technology FLIM systems for ophthalmology FLIM systems for dermatology FLIM systems for tissue imaging FLIM through endoscopes Time-resolved NIRS and fnirs Imaging Online FLIM at rates of up to 10 images per second Please see bh TCSPC Handbook or contact bh FLIM for other Scanning Systems bh FLIM systems can be configured for almost any conceivable laser scanning system. They work with galvanometer scanners, polygon scanners, resonance scanners, and motor-driven and piezo-driven scan stages. Left: FLIM recorded with Lucid Vivascope, utra-fast polygon scanner Right: STED FLIM recorded with STED microscope of Abberior Systems, Goettingen Please see bh TCSPC Handbook or contact bh. 15

16 Technology Leader in Photon Counting New: Wide-Field TCSPC FLIM System Photek MCP detector with delay-line readout Three SPC-150N TCSPC modules with special discriminators Resolution of detector about 250 x 250 pixels Electronic resolution of TCSPC system up to 1024 x 1024 pixels Time resolution approximately 300 ps fwhm Works with Zeiss, Olympus, and Nikon inverted microscopes Exitation by BDS-SM picosecond diode laser Transfer optics and detector attached to left side port System control and data acquisition by bh SPCM software Data analysis by bh SPCImage FLIM analysis software TCSPC Systems for Time-Domain Diffuse Optical Tomography Modular DOT systems Up to 8 parallel TCSPC channels Up to 32 detector channels Up to 8 BDS-MM lasers with wavelength multiplexing Up to 8 wavelengths multiplexed with supercontinuum laser Recording with multiplexed source position Time-of -flight distributions for all combinations of wavelength, source, and detection channels Diffuse correlation in 16 channels Recording by SPC-134, SPC-134EM, SPC-154, or SPC-164 TCSPC packages Laser control by DDG-200 digital delay generator Detector control via DCC-100 cards Saturated count rates up to 40 MHz Multiplexing periods of lasers individually programmable Unlimited sequential recording by continuous flow mode Acquisition rate up to 20 time-of-flight distributions per second New: Non-contact scanning by galvanometer scanner controlled by GVD120 card Fibre-Based Fluorescence-Lifetime Systems for in-vivo Applications Fibre-based TCSPC system for in-vivo application Implantable fibre tip, removable from fibre system Single mode excitation, multi-mode detection Excitation by BDL or BDS ps diode lasers Detection by hybrid detectors or SPAD detectors Multi-wavelength detector option Excellent sensitivity Excellent time resolution Low background fluorescence Detection of NADH, FAD, or exogenous fluorophores 2+ Ca detection in neuronal tissue po2 measurement in live tissue Systems for Recording Optical Emission from Barrier Discharges Recording of full spatio- temporal pulse profiles Multi-dimensional TCSPC process Sinusoidal discharge voltage, 5 to 15 khz Fast galvanometer scanning along discharge gap Control by GVD-120 scan controller Fully integrated in SPCM software Detection of optical signals by one or two detectors Optional 16-wavelength detector Recording by one or two parallel SPC-150 TCSPC modules Online display of data Please see bh TCSPC Handbook, 6th edition 16

17 More than 1600 TCSPC Systems Worldwide TCSPC Instrument Software SPCM Data Acquisition Software for all bh TCSPC Modules and DPC-230 Photon Correlator True 64 bit software for Windows 7, 8, 10 Megapixel FLIM Technology Same software for all bh TCSPC modules and DPC-230 card One software for all operation modes Full access to all functions of the boards Configurable for different instrument configurations Parallel operation of up to four SPC or DPC modules Online display of images and curves Online calculation of FCS, PCH, and MCS traces Online fit of FCS curves Cycle function Page stepping Autosave functions Oscilloscope mode Multi-wavelength fluorescence decay recording Single and repeat mode Sequential modes Imaging modes Single photon parameter-tag mode Hardware-accumulation FLIM mode Software-accumulation (parameter tag) FLIM mode Multi-wavelength FLIM Spatial Mosaic FLIM Temporal mosaic FLIM for accumulation of ultrafast time series Fluorescence lifetime transient scanning (FLITS) Simultaneous recording of FLIM and PLIM Integrated scanner control Integrated detector control Integrated laser control for PLIM Integrated control of Ti:Sa laser and AOM Integrated control of Zeiss Axio Observer Z1 microscope 2D and 3D display modes Image display with software gating Display of multi-wavelength FLIM images 3D Curve mode, colour-intensity display of multi-dimensional data Saving, loading, and conversion of photon distributions, images, and time-tag data Loading of instrument configuration via predefined setups 2D and 3D data processing functions Automatic interaction with SPCImage FLIM data analysis Runs under Windows 7, Windows 8, Windows 10 The SPCM software is free. Please downloaded from 17

18 Technology Leader in Photon Counting SPCM Data Acquisition Software for bh TCSPC Modules and DPC-230 Photon Correlator One Software - Multiple Applications 18

19 More than 1600 TCSPC Systems Worldwide TCSPC Data FLIM Analysis Software SPCImage FLIM Data Analysis Software Analysis of FLIM data Analysis of PLIM data Analysis of single FLIM or PLIM channels Analysis of multiple FLIM or PLIM channels Analysis of single-curve fluorescence data Analysis of single-curve phosphorescence data Analysis by iterative convolution and fit procedure Analysis by first moment of photon distribution Multi-tread calculation procedure Single, double, and triple-exponential decay models Incomplete decay models Baseline correction Free or fixed lifetimes of decay components Pseudo-global analysis Synthetic, measured or manually defined IRF Extraction of IRF from SHG components in FLIM image Region-of-interest selection Single and double-exponential FRET Display of lifetime images Display of FRET images Display of lifetimes, amplitudes, intensities or ratios of parameters Calculation of FRET efficiencies Histograms of lifetimes, amplitudes, intensities or ratios of these parameters Export of lifetime data Export of images Direct interaction with SPCM software Automatic transfer of SPCM data to SPCImage Transfer of data of selected channels or of all channels New! Processing of Megapixel FLIM data New! Processing of Mosaic FLIM data New! Batch processing of multiple FLIM files New! Batch processing of multi-wavelength FLIM data New! Batch export of FLIM images and decay data New! Multiple region of interest definition New! Two-dimensional histograms of decay parameters Histograms of pixel frequency over two selected parameters of the fluorescence decay Back-annotation of parameter ranges in FLIM images New! Phasor plot Fourier transform of decay data Histograms of amplitude and phase of decay data in frequency space Back-annotation of parameter ranges in FLIM images 19

20 Technology Leader in Photon Counting TCSPC Single-Molecule Burst-Analysis Software New! Single-Molecule Burst- Analysis Software Identification of single-molecule photon bursts in parameter-tag data Analysis of fluorescence intensity within photon bursts Analysis of fluorescence lifetime within photon bursts One- and two-dimensional histograms of burst parameters Filtered histograms of burst parameters Discrimination of different fluorescent species Determination of FRET efficiencies Discrimination of different FRET states Calculation of FCS and cross-fcs FCS fit with user-defined model functions Exclusion of artefacts in intensity traces Time-gating Selection of excitation channels in PIE data DLL and Lab View Libraries for TCSPC and Multiscaler Modules DLL libraries are available for most bh data acquisition and experiment control modules. We recommend, however, to check whether the desired effects can be obtained by using the hardware control features of our modules. Please contact bh for details. 20

21 More than 1600 TCSPC Systems Worldwide TCSPC Literature from the Technology Leader Wolfgang Becker High Performance Photon Counting The bh TCSPC Handbook 6th Edition Wolfgang Becker Advanced Time-Correlated Single Photon Counting Techniques 400 pages, 349 figures, 557 references Springer 2005, ISBN Please contact bh for your personal copy Wolfgang Becker The bh TCSPC Handbook 6h Edition, December 2014, updated September pages, 874 figures, 1007 references Available from Please contact bh for printed copies Becker & Hickl GmbH DCS-120 Confocal Scanning FLIM Systems 6th Edition, April pages, 375 references, 410 figures Available from Modular FLIM Systems for Zeiss LSM 710 / 780 / 880 Family Laser Scanning Microscopes with appendix for LSM 510 systems 6th edition, April pages, 398 figures, 411 references Available from DCS-120 Confocal Scanning Systems Overview brochure 30 pages, 42 references, 41 figures Available from Becker & Hickl GmbH BDL-SMN Series Picosecond Diode Lasers FLIM for Zeiss LSM 710 / 780 / 880 Family Laser Scanning Microscopes Overview brochure 47 pages, 164 references, 58 figures Available from BDL-SMN Series Picosecond Diode Lasers User Handbook Available from DPC Channel Photon Correlator User Handbook April pages, 89 figures, 31 references Available from PML-16-C 16 and PML-16 GaAsP 16 Channel TCSPC / FLIM Detectors PML-SPEC and MW FLIM Multi-Wavelength Detectors User Handbook 61 pages, 75 figures, 99 references Available from Wolfgang Becker, ed. Advanced time-correlated Single-Photon Counting Applications Springer, 2015, ISBN , ISBN (ebook) Please contact bh for your personal copy 21

22 Technology Leader in Photon Counting Design, Manufacturing and Quality Management A Strong Partnership bh and Dorazil form an alliance since Located in the same building, our companies closely cooperate at all stages through schematics design, hybrid circuit design, board layout, and computer-aided manufacturing and testing. Continuous quality management through the complete design and manufacturing flow results in high reliability and short delivery time both for prototypes and large order quantities. Continuous flow from design into manufacturing Hybrid-circuit, FPGA, and PCB design Electronics running off the mill High-density automatic SMD placement Automatic soldering ROHS lead-free Thin film and thick film hybrid circuits In-house manufacturing Automatic placement of components Automatic chip bonding ISO 9001 and ISO certified Quality management though entire manufacturing process Uncompromised quality at any number of pieces 22

23 More than 1600 TCSPC Systems Worldwide International Sales Representatives USA, Canada Boston Electronics Corp UK: Photonic Solutions PLC Japan: Tokyo Instruments Inc. jp China and Hongkong: DynaSense Photonics Co. Ltd. India: Carl Zeiss India Russian Federation: BioVitrum Russian Federation: Azimuth Photonics Korea: Advanced Nano Technologies (ANT Co) Korea: UniNanoTech Co.Ltd. Other Regions: Please feel free to contact bh directly 23