Compatible Visible Spectrophotometer T3/T3M. Operation Instruction Manual

Transcription

1 Compatible Visible Spectrophotometer T3/T3M Operation Instruction Manual

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3 Introduction Thanks for your purchase of the Persee Analytics Inc. T3 spectrophotometer. UV-Visible Spectrophotometer is a well-accepted, documented technique with many applications. The technique is extensively used for the analysis of agricultural products and is widely used in public health, environmental protection, life sciences industries and many other organic and biochemical applications. For obtaining maximum performance and trouble-free operation, we give you the following suggestions: Upon receiving your instrument, please check all the items listed in the packing list are included and install the instrument according to the instruction manual. The spectrophotometer is a precise electronic instrument. Please 1

4 read the instruction manual carefully before operation. If the equipment is used in a manner not specified by the manufacturer, the protection provided by the equipment may be impaired. Avoid collision and handle the instrument carefully during transportation. If the tested solution is harmful to humans, please wear gloves or use other protection methods. Please keep the accessories in a safe place for the use in future maintenance. For more information, please visit 2

5 Notice: The light source of the instrument must be preheated for 10 minutes. It can start measurement after preheating. Please ensure that the battery is charged amply before using the instrument. The charging time should not be too long. Using the touch pen to operate on the touch screen, avoid using edge tool to destroy the touch screen, avoid pressing the touch screen forcibly. Avoid blocking the exhaust fan, avoid working in the high temperature environment. In order to avoid destroying the instrument, strong impact should be avoided. Instrument should be placed in a stable working platform. Strong vibration source should be avoided during the measurement. Take some safety measure arrest the thunder when installing antenna in the wild. It s forbidden to absorb the magnet of the antenna on the object with electricity. Instrument should not work in that environment where the humidity is higher than 85%. 3

6 Instrument should be avoided working in strong electromagnetic environment. Balance the energy after changing measurement assembly. 4

7 Contents: Chapter 1 Introduction 1.1 Uses 1.2 Structures 1.3 Characters 1.4 Functions and configuration 1.4 Specifications and performance Chapter 2 Main units 2.1 Main unit configuration 2.2 Test tube holder accessory 2.3 Cell holder accessory 2.4Main unit and interface introduction 2.5 Connection between the main unit and PC/Accessories 2.6 Power supply monitor and battery charging Chapter 3 Measurement accessories instruction 3.1 Fiber probe 3.2 Using method Chapter 4 Operation 4.1 Instruction before using 5

8 4.1.1 Interface introduction Auto zero Sample measurement 4.2 Operation Main interface Food analysis Water analysis Quantitative measurement Photometric measurement Kinetics Spectrum scanning System settings 4.3 Video Chapter 5 T3 Data Viewer software installation and Operation 5.1 Software installation T3 Data Viewer Installation steps Installation of Microsoft. Net Framework Installation of Windows Mobile Device Center Installation of USB drivers 5.2 Software operation 6

9 Chapter 6 Instrument maintenance 6.1 Maintenance cautions 6.2 Replacement of battery 6.3 Replacement of fiber probe and reflection mirror Chapter 7 Troubleshooting Chapter 8 Instrument Configuration T3 / T3M Instruction Manual 7

10 Chapter 1 Introduction 1.1 Uses T3 is a small portable quick-test instrument, whose measurement speed is very fast. The dimension of the instrument is small, and it s light. It is convenient for carrying and manually operation. It can not only be used in the lab, but also special for the wild environment monitor and on-site real time detection. The instrument is provides with the function of spectrum scanning, kinetics, quantitative measurement and photometric measurement, so the application field of it is so wide. The measuring method is based on the Lamber-Beer s Law, which is found on the absorption to light of the material. 1.2 Structures The T3 system is consist of main unit, cell holders, test tube holders and fiber probe. 1.3 Characters ARM11 high-speed embedded processor and embedded real-time operational system WINCE6.0 are used for the core control part of the T3, which provides 8

11 perfect software and hardware platform for the functions implementations of the instrument. For the convenience of user's operation, extra large display touch-screen is used for display. The monochrometer adopts concave plate holographic grating made by French JY company integrated with CCD ILX511 detector made by Japanese Sony Company featuring with high wavelength accuracy and low stray light. The light source adopts gathering micro tungsten lamp with the characteristics of small volume, stable illumination and long life time, which can meet the special requirements for portable devices. 1.4 Functions and configuration Spectrum Scanning, Photometric Measurement, Kinetics, food analysis, water analysis, video player and other user-defined functions (quantitative measurement). The main unit can be operated stand alone, save and read the data and print directly. High-Lattice touch screen. It can be operated by touch pen or mouse. Communicate by the USB and RS232 port, LAN communication is reserved. Equipped with GPRS/GPS module. Support video play function. 9

12 The optical structure support multi kinds of sampler holders changeable and the light source energy can be adjust automatically. Built-in chargeable battery, it can work for 5 hours with one charging. (with full loaded, it can do precision measurement for 4-5 hours ) Configure with V/50Hz-60Hz charging power supply, it can offer power for the main unit and charge the battery. Offering micro printer. (optional configuration) Offering box for carrying the instrument conveniently. 1.5 Specification and performance Light Source Convergent tungsten lamp, the life time is 7000 hours. Measurement Mode Monochromator Detector Spectrum Quantitative Kinetics Photometric Concave plate holographic grating CCD ILX Pixels 10

13 Measurement parts Power supply Printer Operating system Input/display Specifications T3 / T3M Instruction Manual Immerging fiber probe, 10mm pathlength, 20mm Pathlength (Exchange); cell holders; test tube holders Built-in chargeable battery and one charging for 5 hours work. Charger Micro printer (optional) Embedded operation system, integration 2G-Flash memorizer. With timing function. Large touch-screen, lattice Wavelength range 380nm-800nm Data resolution 0.4nm Spectral bandwidth 4±0.8nm Wavelength accuracy ±1.0 nm Wavelength repeatability 0.1nm Baseline flatness ±0.005Abs Noise 0.5% Drift 1.0% Stray light 0.5% Photometric accuracy ±1.0% 11

14 Photometric repeatability Dimension mm Environmental Operation environment 5-30 temperature Store environment % Chapter 2 Main units 2.1 Main unit configuration Figure 2-1 Main unit 2.2 Test tube holder accessory 12

15 There are two kinds of test tube holder: 1. φ25 test tube holder: shown as figure 2-2. Loose the fixed screws off the hole, insert theφ25 test tube into the test tube holder, and the test tube is fixed by the spring. Figure 2-2 φ25 Test tube holder accessory 2. φ16 test tube holder: shown as figure 2-3. Insert the small test tube adapter into the test tube holder, tighten the screws. Insert theφ16 test tube into the adapter, and the test tube is fixed by the spring. 13

16 Figure 2-3φ16 Test tube holder accessory Cautions: If choosing the special test tube with arrow label it s necessary to rotate for location after inserting the test tube into test tube holder. The two arrows on test tube and test tube holder should be matched as figure 2-4 shown. Figure 2-4 Test tube location 14

17 2.3 Cell holder accessory Shown as figure 2-5. T3 / T3M Instruction Manual Figure 2-4 Cell holder The cell holder is consist of holder, baffle and spring. It supports 10mm, 20mm, 50mm cuvettes. The baffle and spring work together to fix the position of cuvettes, assure the precision of measurement. Installation: 1. Put the cell holder into the sample compartment. 2. Tighten the fixed screw. 3. Insert the baffle and spring into the cell holder as the cuvettes dimensions. 4. Clean the cuvettes after finishing measurement. 5. Loose the fixed screw, and remove the cell holder. 15

18 Figure 2-6 Installation of cell holder 16

19 2.4 Main unit and interface introduction T3 / T3M Instruction Manual SIM Card RS23 GPS interface USB main interface USB slave interface Power switch LAN Charger indicator AC plug Figure 2-2 Main unit and interface Power switch (ON/ OFF): Turn on the main unit and the instrument is running. AC Plug: Connecting with the charging power supply, power for the main unit and charge the battery. Charging indicator: The indicator is lit when charging the main unit, it is off 17

20 when the charging is finished. RS232: The interface is connected with printer and accessories through special communication cable. USB main interface: Support the flash memory, saving the data into the flash memory. Also can connect with the USB printer. USB slave interface: Connect with the PC. Download, view, export and print the data by the special software T3 data viewer. SIM card: Insert the SIM card for GPRS communication. Here is the insert method of the SIM card as figure 2-8 shown. Figure 2-8 SIM card insert method LAN: Connect with the LAN for internet communication. GPS interface: Connect with the GPS antenna to acquire GPS data. 18

21 2.5 Connection between the main unit and PC/Accessories The RS232 interface is connected as Figure 2-9. One side of the special communication cable is connected with the RS232 port, while the other side is connected with the printer. Print the data and spectrum directly by pressing Print button on different modules. The communication method between the T3 and PC is the same as that mentioned above. One side of the USB cable connected with the USB slave interface, while the other side is connected with the PC, the communication will be built after synchronization. 19

22 Figure 2-9 RS232 port communication 2.6 Power supply monitor and battery charging Charge interface is connected as Figure

23 Figure 2-10 Charging interface connection When the main unit is running, it will display the battery volume on the right upper corner. See figure

24 Figure 2-11 Power display There will be some notice if battery is low. See figure Please use the charging power supply for offering power to the main unit and charging the battery. The charging power supply should be connected with AC of V/50-60Hz. The other end is connected with the charging interface on the main unit. The power supply indicator is lit. The screen displays charging. After the charging is finished, the power supply indicator will be off. The screen displays. The standard charging time is 5-6 hours. The 22

25 recommended charging time should not be too long to avoid damage the battery. The charger specification is V/50-60Hz input and 18.5V/3.5A output. Figure 2-10 Low battery Chapter 3 Measurement accessories instruction Note: The flexing diameter of the fiber should be bigger than 0.1 meter to avoid the inner fiber breaking. Fix the fiber probe in the holder when measuring, and keep it not moving. 3.1 Fiber probe This instrument takes fiber probe to take photometric measurement. Put the immerging fiber probe into the sample solution to measure. Here are the 23

26 specifications of fiber probe: Wavelength range: nm Pathlength: 10mm, 20mm (interchangeable) Standard SMA 905 port Attached with holder, easy to install on site. The reflection mirror can be disassemble, easy to clean The material of the fiber probe is titanium alloy, which is acid and organic solvent resistant. Figure 3-1 Fiber probe assembly 24

27 3.2 Using method The branching ends of the fiber probe connect with the light source export and sample light import respectively, choose different path length according to different measure method. Insert the fiber probe into holder. Measurement: choose the corresponding method, put the fiber probe into a blank solution to do zero correction and sample to measure. Clean the probe with a filter paper and wash with the solution to be test three times. Note: If there is bubble existing on the top of the probe after inserting into the solution, please remove the tested solution and shake the handle gently. The measurement can t be done until the bubble is removed 25

28 Chapter 4 Operation 4.1 Instruction before using Interface introduction There are four parts of the interface, which are status bar, navigation bar, current page and tool bar. See figure 4-1. Status bar Navigation bar Current page Tool bar Figure 4-1 Interface Status bar: Shown on the left of the status bar are the current measurement mode and flash memory notice, the choices are transmittance (T%), Abs and Energy (Es), on the right is the current time and battery status. 26

29 Current page: The current page is switched by the navigation bar. Navigation bar: In the middle of navigation bar is the current measurement, shown as figure 4-1 is Spectrum scanning. The button on the both sides can be used to switch between different pages. Go back to the measurement page by pressing button. Only on measurement page can switch to the other navigation pages. Tool bar: List the operation that can be carried on the Current page. The chosen items character will be bold. Taskbar: A dynamic window pop-up when a certain task is performed, the window automatically disappears after the task is completed. Click to abort the task and hide the window. There are also the buttons as follows, the Figures below are only for examples, the contents displayed will change in application. Multiple choice button: 27

30 Click the option you want to select, the background turns blue. If the color doesn t change after clicked, then it means you cannot select this option. Multiple choice drop-down box: Click the drop-down arrow, the following option box, click the option you need. Figure 4-2 If there is in the option box, click it will pop up the input panel, by which the user can customize the option. Input & edit box: If you want to change the content in the input box, click the content on the right, the program will automatically detect the content input, and a corresponding number or text input panel will pop up for user to edit. Numerical /Text Input Panel: 28

31 Figure 4-3 Figure 4-4 Figure 4-3 is the numerical input panel, Figure 4-4 is the text input panel. For Figure 4-3 0~9 Numerical input keys; Enter the decimal point; Clear the input, re-enter; Delete the data-in front of; Save the original data value and exit; 29

32 Save the new input and exit. For Figure 4-4: 26 letters; Shift the input methods among characters, numbers, and PinYin; Space; Next line; Delete the date-in front; Previous line; Save the new input and exit; Save the original data value and exit. Spectrum Processing: 30

33 If the main page is spectrum, double-click the spectrum, a spectrum processing box will pop up as follows: Figure 4-4 The functions of the buttons are as follows: Zoom in; Zoom out; Peak search; 31

34 Translate; Full screen; Read screen point data; Change the range of coordinates; Distance measuring, positioning by dragging the acute angle of the triangle; Read the point value on the spectrum, if click this button, use the arrow moving from left to right to select the date point; Select the area of interest; Move the whole processing panel; Exit the spectrum processing. 32

35 4.1.2 Auto Zero Before measuring the transmittance (T%) and absorbance (Abs), the use needs to carry out the zero calibration. The steps are as follows: Step one: put in the blank solution, press the button <zero>, which reminds you to put in the blank solution, Figure 4-6 Then press the button <OK>, the following taskbar will pop up: Figure 4-7 Step two: if the Auto Zero is successful, the taskbar will disappear automatically Sample Measurement After the auto aero is finished, put in the solution you want test, click <measure> on the main page, a taskbar <measuring> will pop up. Click to abort the measurement. The taskbar will hide automatically when the measurement is finished. 33

36 The process of auto zero and measurement will not be mentioned again in the following description. 4.2 Operation Main Interface Switch on the mainframe, the main interface (see Figure 4-8) will display after the boot screen. Figure 4-8 Eight functions of this unit: food analysis, water analysis, quantitative measurement, spectrum scanning, photometric measurement, kinetics scanning, system settings, and method videos. This unit is touch screen control, press the key areas with touch pen or mouse 34

37 will perform the corresponding functions. The following is the introduction to the six functions Food analysis This module includes more than 50 methods of food analysis: nitrite, sulfur dioxide, formaldehyde, cyanide and peroxide value, etc. User can also customize dedicated test methods (under "quantitative measurement" mode). Ordinary procedure of <food safety>: select measuring methods-select measuring module-measure sample-save data-check data-print data. (Refer to<instruction manual of food safety analysis> for more information). Fig4-9 is the method-selection interface of <food safety>. 35

38 Figure 4-9 Choose the method, the description characters of this method will turn into deep color, click" start measuring, at first, it will remind you to choose appropriate measurement modules, see fig Figure 4-10 Click "OK", enter into the measurement interface shown in fig

39 Figure 4-11 The operations user can be done on the measuring main page are: Zero: zero before measurement. According to prompt: put in the blank solution, return to the measuring interface to measure after zeroing. Start: begin to measure. Press <Measure>, the system will carry out the real-time measuring. Save: Save the measuring results. Press <Save>, a text box will pop up in which the user can input the name of the data. If GPRS is on, the data the relevant information will be uploaded in the mean time. Timer: start the predefined timer. Exit: back to the main page. 37

40 Quantity: input the mass or volume of sample. Dil. Factor: If the concentration exceeds the concentration limit, it can be diluted before the test, as a baseline for measurement. Click the on the navigation bar to set the parameters. The interface as Figure 4-12 will pop up, which consist of parameter option page and instrument option page. Parameter Setting Page Figure 4-12 The options can be set up are: Coefficient: Input the factors of the calibration curve, different factors for different methods 38

41 Concentration range: display the upper and lower limits of concentration of this method. Timers Count: modify the number of timers and the time for them. Significant figure: number of significant digits displayed. Instrument Setting Page Figure 4-13 Average times: can be selected from 1, 2, 4, 8 to

42 Lamp Setting page Figure 4-14 Lamp on/lamp off: set the status of the lamp. Energy balance: adjust the energy of tungsten lam. (After changing measurement assembly click this button to satisfy requirement) 40

43 Sample information page T3 / T3M Instruction Manual Figure 4-15 Sample Ent. : set the company of sample belongs to. Sample Name: set the name of measured sample. Operator: set the name of operator. Enter the calibration standards page by clicking the button on the navigation bar, then measure the standard sample. 41

44 Figure 4-16 Add: add a new standard sample, you will first input the concentration of the standard sample as figure 4-17 shown. 42

45 Figure 4-17 Zero: Zero before measurement. Start: begin to measure. Delete: delete the selected standard sample. Apply: apply the method and save. Return: return to the setting interface By shift between Standard and Curve can view the related data and fitting information. See Figure 4-18 for the standard curve. Note: in case the first order curve is used, at least two sets of standard samples should be measured, in case quadratic curve is used, at least three sets of standard samples should be measured. 43

46 Figure 4-18 Curve Click the button in the navigation bar on the measurement interface can view the previous measuring results corresponding to the current method. 44

47 Figure 4-19 Delete: select and delete the certain data. Print: pint the selected data. All/None: select all the data. If all is selected the current option will be None, which can clear the options. Export: insert the flash memory into the USB slot, the user can export the data to it. Upload: upload the chosen data to the server. Besides, description should be added for the "pesticide residue" method. The following figure is the measurement interface of "pesticide residue": 45

48 Figure 4-20 Reference: measure the reference solution, then get the value A1 of reference solution, timer begin to count down. Sample: measure the sample solution, then get the value A1 of sample solution. Put the reference solution into test tube holder when the timer is to finish counting down, the value of A2 of reference solution will be automatically read out. Put the sample solution into test tube holder, then the value of A2 and inhibition ratio will be obtained. 46

49 4.2.3 Water quality analysis This module includes more than 30 methods of water quality analysis: Cr VI, cyanide, free chlorine and sodium reagent ammonia, etc. User can also customize dedicated measuring methods (under <quantitative measurement> mode) The ordinary procedure of <water quality analysis>: select measuring methods-select measuring module-measuring sample-save data-check data-print data (Refer to <instruction manual of water quality analysis> for more information). Figure 4-21 Choose the method, the description characters of this method will turn into 47

50 deep color, click" start measuring, at first, it will remind you to choose appropriate measurement modules, see fig Figure 4-22 Click "OK", enter into the measurement interface shown in fig

51 Figure 4-23 The operations user can be done on the measuring main page are: Zero: zero before measurement. According to prompt: put in the blank solution, return to the measuring interface to measure after zeroing. Start: begin to measure. Press <Measure>, the system will carry out the real-time measuring. Save: Save the measuring results. Press <Save>, a text box will pop up in which the user can input the name of the data. If GPRS is on, the data the relevant information will be uploaded in the mean time. Timer: start the predefined timer. Exit: back to the main page. 49

52 Dil. Factor: If the concentration exceeds the concentration limit, it can be diluted before the test, as a baseline for measurement. Click the on the navigation bar to set the parameters. The interface as Figure 4-24 will pop up, which consist of parameter option page and instrument option page. Parameter Setting Page Figure 4-24 The options can be set up are: Coefficient: Input the factors of the calibration curve, different factors for different methods Concentration range: display the upper and lower limits of concentration of this 50

53 method. Timers Count: modify the number of timers and the time for them. Significant figure: number of significant digits displayed. Instrument Setting Page Figure 4-25 Average times: can be selected from 1, 2, 4, 8 to

54 Lamp Setting page Figure 4-26 Lamp on/lamp off: set the status of the lamp. Energy balance: adjust the energy of tungsten lam. (After changing measurement assembly click this button to satisfy requirement) 52

55 Sample information page T3 / T3M Instruction Manual Figure 4-27 Sample Ent. : set the company of sample belongs to. Sample Name: set the name of measured sample. Operator: set the name of operator. Enter the calibration standards page by clicking the button on the navigation bar, then measure the standard sample. 53

56 Figure 4-28 Add: add a new standard sample, you will first input the concentration of the standard sample as figure 4-29 shown. 54

57 Figure 4-29 Zero: Zero before measurement. Start: begin to measure. Delete: delete the selected standard sample. Apply: apply the method and save. Return: return to the setting interface By shift between Standard and Curve can view the related data and fitting information. See Figure 4-30 for the standard curve. Note: in case the first order curve is used, at least two sets of standard samples should be measured, in case quadratic curve is used, at least three sets of standard samples should be measured. 55

58 Figure 4-30 Curve Click the button in the navigation bar on the measurement interface can view the previous measuring results corresponding to the current method. 56

59 Figure 4-31 Delete: select and delete the certain data. Print: pint the selected data. All/None: select all the data. If all is selected the current option will be None, which can clear the options. Export: insert the flash memory into the USB slot, the user can export the data to it. Upload: upload the chosen data to the server Quantitative Measurement The quantitative measurement is user-defined, the procedures for <quantitative 57

60 measurement> are: New measuring measure the standard sample measure the sample save data view data print data/export data. See Figure 4-32 for the interface of quantitative measurement, Figure 4-32 Quantitative The operations user can be done on the measuring main page are: Zero: zero before measurement. According to prompt: put in the blank solution, return to the measuring interface to measure after zeroing. Start: begin to measure. Press <Measure>, the system will carry out the real-time measuring. Save: Save the measuring results. Press <Save>, a text box will pop up in which 58

61 the user can input the name of the data. If GPRS is on, the data the relevant information will be uploaded in the mean time. Timer: start the predefined timer. Exit: back to the main page. Dil Factor: If the concentration exceeds the concentration limit, it can be diluted before the test, as a baseline for measurement. Click the on the navigation bar to set the parameters. The interface as Figure 4-33 will pop up, which consist of parameter option page and instrument option page. Parameter Setting Page Figure 4-33 Quantitative setting 59

62 The options can be set up are: Single wavelength/dual wavelength: set the measurement wavelength 1 st Order/ 2 nd order: times of fitting Coefficient a, b, c: in order, coefficient of constant, the first degree polynomial, and the quadratic degree polynomial. Concentration range: display the upper and lower limits of concentration of this method. Timers Count: modify the number of timers and the time for them. Significant figure: number of significant digits displayed. Unit: Display or set the unit of the method. +ve Slope/-ve Slope: display whether to choose fading measurement or not, among which the values of absorbance, slope, and correlation coefficient are all negative (COD low concentration). 60

63 Instrument Setting Page T3 / T3M Instruction Manual Figure 4-34 Average times: can be selected from 1, 2, 4, 8 to

64 Lamp Setting Page Figure 4-35 Lamp on/lamp off: set the status of the lamp. Energy balance: adjust the energy of tungsten lam. (After changing measurement assembly click this button to satisfy requirement) 62

65 Sample information page T3 / T3M Instruction Manual Figure 4-36 Sample Ent. : set the company of sample belongs to. Sample Name: set the name of measured sample. Operator: set the name of operator. Save method: use can save the current method as user-defined method on measurement page. Input the name in the pop-up text box. Calibration standards: enter the calibration standards interface Enter the calibration standards page by clicking the button on the navigation bar, then measure the standard sample. 63

66 Figure 4-37 Add: add a new standard sample, you will first input the concentration of the standard sample. 64

67 Figure 4-38 Zero: Zero before measurement. Start: begin to measure. Delete: delete the selected standard sample. Apply: apply the method and save. Return: return to the setting interface By shift between Standard and Curve can view the related data and fitting information. See Figure 4-39 for the standard curve. Note: in case the first order curve is used, at least two sets of standard samples should be measured, in case quadratic curve is used, at least three sets of standard samples should be measured. 65

68 Figure 4-39 Curve Click the button in the navigation bar on the measurement interface can view the previous measuring results corresponding to the current method. 66

69 Figure 4-40 Delete: select and delete the certain data. Print: pint the selected data. All/None: select all the data. If all is selected the current option will be None, which can clear the options. Export: insert the flash memory into the USB slot, the user can export the data to it. Upload: upload the chosen data to the server. Click the button on the navigation bar can view all the customer-defined methods save in by the user. 67

70 Figure 4-41 Load method: replace the current parameters setting with parameters for the selected methods and enter the measuring interface. Delete: delete the selected method. Delete all: clear the list of all the methods. Note: if the user does not select the method, when the quantitative measurement is opened, the default parameters will be those of previous measurement Photometric Measurement Photometric Measurement means to read the measuring data at the certain wavelength. That is to read the data at a fixed point, the user can decide a 68

71 wavelength point and do the photometric measurement. The process is: set measuring parameters measure samples save data view data print data. Figure 4-42 See Figure 4-42 for photometric measurement interface. Press button into setting page, see Figure

72 Figure 4-43 Parameter Setting Page Abs/T%/Es: choose the working mode between Abs, T% and Es. Wavelength: input the value of a wavelength point. Coeff. K: the value displayed in the column K result is the result produced by point value measured multiplying this value. +ve Slope/-ve Slope: display whether to choose fading measurement or not, among which the values of absorbance, slope, and correlation coefficient are all negative (COD low concentration). Instrument Setting Page 70

73 Figure 4-44 Average times: can be selected from 1, 2, 4, 8 to 16. Integrating time: set the integrating time, the longer of integrating time, the higher of energy. Gain: set the amplifier times. Lamp Setting Page 71

74 Figure 4-45 Lamp on/lamp off: set the status of the lamp. Energy balance: adjust the energy of tungsten lam. (After changing measurement assembly click this button to satisfy requirement) Sample information page 72

75 Figure 4-46 Set the information of sample Press button to enter the view page and view the saved photometric measurement results. 73

76 Figure 4-47 View: return the measuring interface to view the measuring value for the current file Kinetics Kinetics is a measuring method in which the sampling is done continuously at a certain time interval, and the sampling data is displayed in graphics. This method is mainly applied to observe the trend of changes of the samples over time. The process is: set measuring parameters measure samples save data view data print data. See Figure 4-48 for the measuring interface of kinetics scanning. 74

77 Figure 4-48 See Figure 4-49 for setting interface. 75

78 Figure 4-49 Parameter Setting Page Abs/T%/Es: choose the measuring mode between Abs, T% and Es. Wavelength: input a specified wavelength point value Interval: time interval between each sampling. Total time: total scanning time, no less than the time interval Y range: coordinates displayed on Y axis during scanning. +ve Slope/-ve Slope: display whether to choose fading measurement or not. Instrument Setting Page Average times: can be selected from 1, 2, 4, 8 to 16. Integrating time: set the integrating time, the longer of integrating time, the higher of energy. Gain: set the amplifier times (under Es mode). Lamp Setting Page Lamp on/lamp off: set the status of the lamp. Energy balance: adjust the energy of tungsten lam. (After changing measurement assembly click this button to satisfy requirement) Sample information page Set the information of sample Press button to enter the view page and view the saved measurement 76

79 results. T3 / T3M Instruction Manual Figure Spectrum Spectrum means scanning a certain spectral range at a certain wavelength interval and displaying the measured data in two-dimensional graphics for further analysis and research. The process is: set measuring parameters measure samples save data view data print data. See Figure 4-51 for the measuring interface of spectrum scanning. 77

80 Figure 4-51 See Figure 4-52 for setting interface. New buttons added to the instrument setting page for the user to input the start wavelength, end wavelength and scan Interval. 78

81 79 T3 / T3M Instruction Manual

82 Figure 4-52 See Figure 4-53 for Browse interface. 80

83 Figure 4-53 View: the selected spectrum will be displayed on the measuring page see Figure Setting The following is the interface of setting: 81

84 Figure 4-54 Setting Functions of these buttons are as follows: Date Time Set the system time by inputting the time. 82

85 Figure 4-55 Lock When locked, the user need to enter the password to unlock the instrument, or it cannot be operated (if GLP is enabled). Figure 4-56 GLP This instrument is equipped with GLP functions. There are three parts of GLP functions: user manager, Log manager, GLP settings. User manager Different authorities for different user group, different password for different users. The user need input this password when power on the instrument and 83

86 unlock it when the GLP function is ON. (The default password for administrator is ). Figure 4-57 Here is the User manager interface. 84

87 Figure 4-58 Add Group: there will be another row after adding, click different table in this line, the edit panel will pop up to edit the information of this group (Only allowed the administrator to carry on this operation). Delete Group: delete the chosen group (Only allowed the administrator to carry on this operation). Group Authority: set the using authorities of chosen group, select means the user group can do the corresponding operation (Only allowed the administrator to carry on this operation). 85

88 Figure 4-59 Group member: list the user of this group, choose the table of this list to edit user s information and password, add new user, delete user. 86

89 Figure 4-60 Log manager All the operation will be record into the log. Click Log to view. 87

90 Figure 4-61 Enter this page can view the user information. GLP Setting If the user belongs to the administrator group, Setting button will be visible which can be used to control the GLP function ON and OFF, and set the owner organization of this instrument. While which is invisible to the user not belong to the administrator group. 88

91 Figure 4-62 Volume Volume setting is used to adjust the volume of the system. 89

92 Figure 4-63 Location To obtain the location information is one of the functions of this unit. There are two methods to locate, GPS and GPRS. Figure 4-64 GPRS Location: Make sure SIM card is inserted and click "GPRS" to locate by GPRS. It will remind you base station code and signal quality after location is completed. GPS Location: Make sure GPS vehicle-mounted external antenna is well connected, click "GPS", then it starts GPS location. Longitude and latitude will be displayed after location is completed. 90

93 Warning at startup: after selecting Warning at startup, the instrument will remind you whether to locate again during each star-up, see fig Figure 4-65 Notice: Make sure the SIM card is recharged when using GPRS location function. Make sure SIM card is firmly inserted, be aware that notched direction heads down. Power off when installing or replacing the card. Make sure the antenna is firmly connected and place the instrument in an area with good signal when using GPS function, avoid interruption and shielding. Put the antenna of the GPS into the GPS holder, and keep the black side upward. If the unit is used indoor, put it close to the windows and put the GPS antenna out of the window. 91

94 GPRS This unit can upload data by GPRS. If this function is enabled, when the measured data is saved on food analysis, water analysis, quantitative mode, it will be uploaded to the wanted address automatically. Figure 4-66 Notice: Likewise, make sure SIM card is recharged when using GPRS. Make sure SIM card is firmly inserted, be aware that notched direction heads down; Switch off the power supply when installing or replacing the card. Printer Printer Setup screen will list the printer that once connected to the instrument. A printer needs to be selected as the default printer. If the printer has never 92

95 been changed, then it is no need to modify the choice again. Figure 4-67 Calibrate If the screen fails to response the clicks correctly, the user can enter into this module and click the centers of the small crosses to calibrate the screen. Debug Mode Debug Mode is used for the after-sale service personnel to maintain the instrument. Input the password will enter the maintenance mode, the password is not open to the customer. 93

96 PC Mode Only on PC mode the T3 data viewer can download data from T3 and update. Click PC Mode it will ask you: Click OK, it will show you: Figure

97 Reset Reset the instrument. Figure

98 Figure 4-70 About Get the Serial Number, Software name, Software version, manufacture information and so on from here. 96

99 Figure Video Method videos of food analysis and water quality analysis can be viewed under this module. The interface of <Method video> is shown as follows: 97

100 Figure 4-72 Play video Pause Video back Video forward Stop Full screen Volume up Volume down Quit the interface of method video The list of video files can be viewed after pressing the button "Browse", the 98

101 format is wmv. Select relevant video files, and see the files after clicking "View". Figure

102 Chapter 5 T3 Data Viewer Installation and Operation T3 data viewer software can be run under Windows2000/XP/Vista/Windows 7, which is used to communicate between PC and T3. The software can read the data from instrument, process data and print them out. T3 data viewer can read the data in the flash memory copied from T3 main unit. T3 data viewer can export the measured result or the spectrum to text document (.txt or.csv) T3 data viewer can print the measured result or the spectrum by the printer connected with PC. T3 data viewer can be used to update the instrument. 5.1 Software installation: T3 data viewer must be run on the PC with following software: Microsoft.net Framework 2.0 Windows Mobile Device center provides the device manager and data synchronization function between mobile device and PC. USB drivers used to recognize T3 main unit which is connected with PC by USB cable. 100

103 5.1.1 T3 data viewer Installation steps Insert the T3 data viewer software CD disk into CD driver of PC, and double click ViewerSetup.exe, the following figure will show: Figure 5-1 The software will ask you to install the Microsoft.net Framework 2.0 if without it. Continue to install the T3 Data Viewer software after installing Microsoft.NET 101

104 Framework 2.0. Press Next it will show you: Figure 5-2 Click Browse to choose the installation directory, press Next it will show you: 102

105 Figure 5-3 Press Next to continue, it will show the following figure after the installation is finished. 103

106 Figure 5-4 Press Close to finish the installation Installation of Microsoft.Net Framework 2.0 Find the Microsoft.NET Framework 2.0.exe installation program from the CD disk, and install as the prompt Installation of Windows Mobile Device Center On Windows 2000/XP system synchronization between the main unit and PC is 104

107 achieved by Microsoft ActiveSync software. While on Windows Vista or Windows 7 is achieved by Windows Mobile Device center Installation of USB driver The USB driver should be installed for the Windows XP OS and lower version. When connect with the PC for the first time, the system will ask to install the driver if needed. Finish the installation by auto searching driver program from the disk. 5.2 Software operation Start Start the T3 data viewer software by the following solution. Double click the T3 data viewer v1.0 icon from the desktop to start. Start-Program-T3 data viewer v1.0 Double click the T3 data viewer.exe from the installed file. There are four parts for the software interface: menu, toolbar, document list, data browsing page. There are six functions of the data browsing page: spectrum, kinetics, quantitative measurement, food analysis, water quality analysis, photometric measurement, which are used to browse and process the data with corresponding type. The main interface is shown like this: 105

108 Figure Download data Open the download window by choosing [download data] from [file] or clicking from toolbar. Before download the data, make sure the connection between the main unit and communication cable is correct, and the synchronization is achieved 106

109 between the main unit and PC. T3 / T3M Instruction Manual Figure 5-6 If the Windows Mobile Device Center has been installed, the system will notice that the device is on synchronization when the instrument connecting with PC. Press [Import index] button to download data index from instrument. Press [Import All] to download all the data to default path when the download of data index is finished. Or choose some data and press [Import chosen items] to download. Save the data file as default name or other name File processing On the left of the main interface is file list. The software will list all the data file 107

110 in the default download path as tree form when started. Here is the basic structure of file list: Instrument model File name Measurement data type Method type Measurement data Measurement data Figure 5-7 Double click the measurement data or choose the data and click button to open it. It will show you the saved path, simple description and create time if the cursor hovering on the File name. By choosing [File]-[Open index] to add the data into the list if the data are saved in the flash memory or the other path of the PC. See the following picture. 108

111 Figure 5-8 Note: Keep the original file structure when moving the data, which means the index and the storage file should be in the same file, and the storage file can t be renamed, or the index cannot find the corresponding measurement data Browse Spectrum/Kinetics data The page structure for Spectrum and Kinetics are the same, which can display spectrum, measurement parameters and peak picking results. Spectrum can be operated and analysis by the T3 data viewer software. Figure 109

112 5-9 is the operation menu and tool bar. Figure 5-9 Axis Range: Setting the display range of X-axis and Y-axis. Area Selection: View the local spectrum by drafting the cursor. Full View: Display spectrum on full screen. Move: Moving the spectrum by holding the cursor. Ruler: Distance measuring, positioning by dragging the acute angle of the triangle. Crosshair: Read the point data by moving the plus cursor on spectrum. Read Value: The horizontal line and vertical line will show on the spectrum, the cross point of horizontal line and vertical line will move to the peak or valley 110

113 nearby by clicking the spectrum by the mouse. Or read the spectrum data continuously by the left and right key of keypad. Peak Pick: Show the peak value on spectrum, and show the result of peak picking on the list. Adjust the peak picking result by changing Peak Threshold / Valley Threshold. Set up the display of peak picking result on the list by Show peak / Show valley which is shown as figure Zoom in: Zoom in spectrum automatically. Zoom out: Zoom out spectrum automatically. Figure

114 5.2.5 Browse food analysis/water analysis/quantitative measurement data The page for food analysis, water analysis and quantitative measurement are the same. Display the calibration curve, parameters and measurement result on Quantitative page. Also can be printed and saved. When open several measurement data with the same calibration method, new lines will be add to the measurement result list. When open with different calibration method, the measurement result list will be displayed from the first line. When open multi files, stop browsing or printing by choosing Edit - Delete current Row s File Data. The interface is shown like this: 112

115 Figure Browse photometric measurement result Display measurement result and parameters on the Photometry page, which is shown as the following figure. 113

116 Figure 5-12 Stop browsing or printing by choosing Edit - Delete current Row s File Data when browsing data, this operation will not delete data Export data: Open the export wizard by choosing File - Data export or clicking on toolbar. If current page is peak picking status, choose the export type first, which is shown as the following figure. 114

117 Figure 5-13 Press Next to set the file format and path, which is shown as the following figure. 115

118 Figure 5-14 Press Export after finish setting up. The T3 Data Viewer software support two types of file, which are ASCII file and text file. They all can be opened by WordPad program of Windows. And the text file also can be opened by Microsoft Excel Print Set up print parameters by choosing File - Print Setup, which is including page setup, which including content setting and page setting. For the content setting, the report setting can be edit, such as the title, author and sample information can be edited. See the following figure. 116

119 Figure 5-15 Figure 5-16 is page setting, including page setting and printer setting. 117

120 Figure 5-16 Print by choosing File - Print or clicking of toolbar. 118

121 5.2.9 Program Update Select menu [Help]-[Update Device] to update the program of the instrument. (Make sure the instrument is successfully connected with PC, and under "PC" mode). See the picture below: Figure 5-17 Current version: The software version of the instrument will be displayed after the instrument is synchronous with the PC. Updated version: The software version needed to be downloaded will be displayed after the instrument is synchronous with the PC. 119

122 Browse: Select the update program. Recover to previous version: If update is not needed, the version can be recovered to previous one. Link to device: Link PC and the instrument, error warning will be displayed if synchronization fails. Click "Browse", select the update program needed. Then, click "Update" to start. Figure 5-18 After the update is completed, the interface shown below will appear. Restart the instrument, and the new program is launched. 120

123 Figure

124 Chapter 6 Instrument maintenance 6.1 Maintenance cautions The main unit and accessories should be put into a convenient box and be locked for transportation. The immerging probe should be cleaned with distilled water and be covered with a rubber cap after the operation. After long-time use, the inner surface of the probe maybe has some filth. Remove the tip of the probe and clean it with a alcoholic cotton tack. It should avoid dust covering the surface of the reflecting mirror after the fiber probe is used. If the operation is not done for a long time, please turn off the power supply of the lamp to save the battery energy. After the charging is finished (the indicator is off), please turn off the power supply, to ensure the battery life and the safety of the instrument. Temperature range: operation environment is 5-30 and store 122

125 environment is Replacement of battery The effective charging times of the rechargeable batteries inside the instrument are The procedure of the replacement of batteries (purchasable from the manufacturer) is as follows: 1. Open the battery cover on the back of the main unit, see fig.6-1, get out the batteries (if the batteries are stuck, make the batteries downward and shake it, then the batteries can be taken out) Battery cover Figure Pull out the battery lead, attention: Curl the lead and slip it inside the enclosure when installing the lead into the batteries. Drag out the lead carefully with a screwdriver. Finally, drag out the lead from the host interface, see fig

126 Figure 6-2 Figure Connect the the power cable of the new batteries with the main unit, put the cable into the battery box carefully, cover the battery holder (see fig.6-3). Notice: Curl the power cable and put the rest of the cable into the main unit, then put the batteries inside the holder, cover the main unit. Tight the 4 fixed screws. 6.3 Replacement of fiber probe and reflection mirror Replacement for fiber detector: if the fiber probe is broken or destroyed, please contact the manufacturer for replacement. The trouble phenomenon is that the energy scanning reading is close to zero when the lamp is lit. 124

127 Replacement for fiber detector top: After the fiber probe has been used for a long time, the energy is lowered evidently. The trouble phenomenon is that the energy reading at 560nm is lower than 10 when the gain is 1 and integrating time is 0.005s in energy scanning. Please contact the manufacturer for replacement. The replacement methods are as follows: The valid time for the fiber detector top is 2 years. When the energy is lower, please contact the manufacturer for replacement. Figure 6-4 Replacement for fiber detector top 125

128 Chapter 7 Troubleshooting No. Phenomenon Trouble-shooting The touch-screen is not 1. Restart 1. accurate 2. Re-orientate the touch-screen 1. Energy balance. 2. Tungsten lamp and power switch broken. The energy from the light Very low energy and bad baseline, source is low. replace with a new tungsten lamp. 4. Check if there is something block the light in the cell holder. 1. Energy balance, blank correction. 2. Check if the sample is turbidity and if there is besmirch in the sample The noise of the chamber for the fiber. 3. instrument is too large. 3. Check if the power for the battery is enough. 4. Check if the power supply is stable when using charger for battery 126

129 charging. 5. Scanning the energy on the Kinetics mode, replace with a new tungsten lamp if the energy fluctuates. 1. Check if the power supply is stable or The menu flashes after the power for the battery is enough. 4. turning on the 2. Turn on the instrument again and run instrument. the software. 1. Do the baseline correction again. 2. Ensure that the cell holder is fixed. Baseline flatness is over 3. Check if the power for the battery is 5. the limitation. enough. 4. Check if the energy is too low, to do the energy balance. 1. Check if the power for the battery is The drift of the 6. enough. instrument is big. 2. Repeat zeroing after warming up. The wavelength of the Return the instrument to the 7. instrument is not precise. manufacturer for servicing. 8. It suddenly shut down 1. Check if the power for the battery is 127

130 during operation. enough. 2. Turn off the main unit and turn it on again. 9. Initialization error Restart 1. The charging indicator should be lit (If not, contact the manufacture.). 10. Charging error 2. Check if the charger is correct. 3. Check if the connector of charger is correct. 4. Replace the battery 11. Cannot enter into main Restart interface Check the flash memory. Export to flash memory 2. Check the connection error memory is correct or not. of flash Check whether the USB cable is The synchronous normal; between instrument 2. Make sure the instrument is under PC and PC error, cannot mode; export the data 3. Make sure the synchronous software 128

131 of Microsoft ActiveSync and USB driver are installed in the PC; 4. Unload and re-install Microsoft ActiveSync and USB driver; 1. Make sure the SIM card is recharged; 2. Check whether the surface of the SIM The measurement data card is clean and not damaged; 14. cannot be uploaded or 3. Check whether the SIM card is GPRS location unavailable correctly inserted into the slot; 4. Turn off the main unit, and insert the SIM card again, restart the instrument; 1. Check whether the external antenna of the GPS is damaged; 2. Check whether the antenna of the 15. GPS location unavailable GPS is firmly installed; 3. Check whether there is any block or electromagnetic interruption around, make sure implement the GPS location in an open area with good signal; 16. Cannot browse the 1. Check whether the video files are 129

132 videos 17. Cannot print 18. Others wmv format; 2. Make sure the video files are built in the instrument; 3. Check whether the video files are saved in the primary directory in the flash disk; 1. Check whether the selection of "printer setting" in "system setting" is correct; 2. Make sure the setting of the printer is correct; When it goes wrong, please check the troubles step by step. If the troubles can t be settled, please contact your local dealers. Chapter 8 Instrument configuration Note:The following drawings for accessories case are only for 130

133 reference. The final configuration is based on the sales contract. Pipette (1ml) Pipette (5ml) Cell holder Wash bottle Mini triangle stand GPS module GPS antenna USB cable Charger Test tube Cuvettes Test tube φ25mm ph meter Spare parts list Figure 8-1 Main unit Test tube φ16mm 131