columns IonSwift MAX-100 Anion-Exchange Column

Transcription

1 columns IonSwift MAX- Anion-Exchange Column The IonSwift MAX- is a high-capacity anion-exchange column with selectivity similar to the IonPac AS-HC column. IonSwift is a new generation of separation media, which is uniquely designed and engineered for the separation of small molecules including organic acids and inorganic anions. The MAX- column is the first of a series of columns developed using monolith technology and is designed to provide high-speed, high-resolution separations of organic acids and inorganic anions using a hydroxide gradient delivered by an eluent generator. The IonSwift MAX- column is recommended for the fast separation of organic acids and inorganic anions in approximately 5 min using hydroxide gradient elution. The MAX- column can be used in most AS-HC applications, but for optimum resolution of the organic acids and inorganic anions in complex sample matrices, the IonPac AS-HC column is recommended. The MAX- column is available in. 5 mm and.5 5 mm formats. These formats offer the advantage of reduced eluent consumption, providing reduced operating costs. High mass sensitivity can be achieved with both formats providing lower MDLs. The MAX- column can be used at relatively high linear velocity due to its low backpressure. The MAX- Analytical column ( 5 mm) is operated with scale ICS- or ICS-5 systems. The MAX- capillary column (.5 5 mm) is operated with the ICS-5 capillary-scale IC system. Major Column Features and Benefits The IonSwift MAX- is a high-speed, high-resolution column designed for the separation of organic acids and inorganic anions. The MAX- columns provide: Fast mass transfer Fast separations High throughput and improved productivity Long column lifetime Outstanding reproducibility Passion. Power. Productivity.

2 Superior Chromatographic Performance Fast gradient separation of organic acids and inorganic anions in approximately 5 min. Ideal alternative for most AS-HC applications. (For highest resolution and capacity use the AS-HC column). Compatible with the Eluent Generator (EG). High capacity: µeq per column ( 5 mm column). Eluent suppression using the ASRS ( mm) suppressor for the MAX- column ( 5 mm) or ACES suppressor for the MAX- column (.5 5 mm) provides Reagent-Free operation with low backgrounds and enhanced analyte sensitivity. Operate at ambient or elevated temperatures. Column selectivity is optimized for a 5 C operating temperature to ensure reproducible results. Compatible with HPLC organic solvents to enhance analyte solubility, modify column selectivity, or for effective column cleanup. Eluent: Eluent Source: Flow Rate: Inj. Volume:. µl Sample Dilution: : Detection: A. Beer sample 5 B. Beer sample IonSwift MAX-G, MAX- Capillary column. mm KOH for 5 min, 5 mm KOH in 5 min, 5 5 mm KOH in min EGC KOH capillary µl/min Suppressed conductivity, ACES, 5 ma, AutoSuppression recycle mode Figure. Analysis of beer using the MAX- capillary column. 9 Peaks:. Lactate. Acetate. Formate. Butyrate ( ppm spiked) 5. Pyruvate. Chloride. Succinate + Malate*. Sulfate 9. Oxalate. Fumarate. Phosphate. Citrate. Isocitrate. trans-aconitate * Succinate and Malate can be resolved using organic solvent Key Applications The MAX- column is solventcompatible which allows for anionexchange selectivity control and easy column cleanup after the analysis of complex matrices. Typical applications include the determination of organic acids and inorganic anions in diverse sample matrices including: Fruit juices and wines Foods and beverages Fermentation and process solutions Chemical additives Chemical process solutions Wastewater Power plant waters Morphology of IonSwift Monoliths The IonSwift monolith structure is designed and engineered to contain an uninterrupted, interconnected network of channels of a specific, controlled pore size. There are two types of pores: large pores (approximately a micron or larger) for eluent to flow through and small pores (ten to hundreds of nanometers) where most of the separations take place. These large pores allow the eluent to flow through with moderate backpressure, allowing high flow rates for faster analyte separations over traditional packed-bed media. The MAX- column uses a high-capacity monolithic media ( µeq/ column for 5 mm and. µeq/ column for.5 5 mm) with optimized selectivity for organic acids and inorganic anions in a variety of sample matrices. Determination of Carboxylic Acids in Complex Matrices The MAX- column can be used to monitor the organic acid and inorganic anion content in the quality control of foods, wines, and beverages. The MAX- is an ideal column for the fast analysis of organic acids and inorganic anions in approximately 5 min using a hydroxide gradient delivered with an EG. The MAX- column is an ideal alternative to the AS-HC column for most organic acid and inorganic anion applications. For highest resolution and capacity, the AS-HC column is recommended. Figure illustrates the separation of the MAX- column for the analysis of beer. The MAX- column shows excellent peak shapes for the monovalent organic acids. Butyric acid is an important indicator of deterioration of foods and beverages and can be separated easily from pyruvic acid on the MAX- column without the use of solvent in the eluent. Figure illustrates the separation of organic acids and inorganic anions in apple juice using the MAX- column.

3 IonSwift MAX- Column for Organic Acids and Inorganic Anions in Fermentation Broths Examining ingredients in fermentation broths is a valuable tool for process control because many ingredients resulting from metabolic processes have been shown to affect yield or quality of the desired fermentation products. Inorganic anions serve to maintain osmolarity and provide cofactors for biosynthesis. Certain organic acids are waste products, and their accumulation inhibits cell growth or product yields. The high capacity of the MAX- column allows for the determination of a number of organic acids and inorganic anions in fermentation broths. Figure illustrates the separation of common fermentation broth anions using the IonSwift MAX- column with a hydroxide gradient delivered by an EG. IonSwift MAX-G, MAX- Capillary column Eluent:. mm KOH for 5 min, 5 mm KOH in5 min, 5 5 mm KOH in min Eluent Source: EGC KOH capillary Flow Rate: µl/min Inj. Volume:. µl Sample Dilution: : Detection: Suppressed conductivity, ACES, 5 ma, AutoSuppression recycle mode C B A, Samples: A. Fresh Apple Juice B. Commercial Apple Juice C. Commercial Apple Juice Peaks:. Quinate. Lactate. Propionate. Formate 5. Galacturonate. Chloride. Succinate + malate*. Sulfate 9. Oxalate. Fumarate. Phosphate. Citrate. Isocitrate. cis-aconitate * Succinate and malate can be resolved using organic solvent, Figure. Analysis of apple juice using the IonSwift MAX- column. IonSwift MAX-G, MAX- Capillary column Eluent:. mm mm KOH in min, mm mm in min, mm 5 mm KOH in min Eluent Source: EGC KOH capillary Flow Rate: µl/min 9 Inj. Volume:. µl Detection: Suppressed conductivity, ACES, AutoSupppression recycle mode Peaks:. Lactate mg/l. Acetate. Propionate. Formate 5. Butyrate. Pyruvate. Isovalerate. Valerate 9. Chloride. Nitrate. Malate. Carbonate. Malonate. Maleate 5. Sulfate. Oxalate. Phosphate. Citrate 9. Isocitrate. Pyrophosphate Figure. Separation of common fermentation broth anions using the IonSwift MAX- column.

4 Anions Monitored in the Petrochemical Industry The MAX- column is ideal for use in the petrochemical industry due to its ruggedness and superior separation for many anions of interest. The range of anions that can be resolved in a single run provides important information to help troubleshoot problems encountered in the petrochemical industry. The MAX- column can be used to resolve a diverse range of anions for corrosion monitoring (chloride in process waters), characterizing reservoir brines (chloride, bromide, nitrate, sulfate in produced waters), analyzing amine scrubber solutions (heat stable salt anions in alkanolamines); and monitoring wastewaters. Figure illustrates the separation of petrochemical anions using the MAX- column using a hydroxide gradient delivered by an eluent generator. IonSwift MAX- Analytical column ( 5 mm) Eluent:. mm KOH in 5 min, 5 mm KOH in 5 min, 5 5 mm KOH in min Eluent Source: EGC KOH Flow Rate:. ml/min Inj. Volume: µl Sample Dilution: : Detection: Suppressed conductivity, ASRS, mm, ma, AutoSuppression recycle mode Peaks: mg/l. Acetate.9. Formate.. Chloride.. Sulfate. 5. Oxalate.. Phosphate.5. Thiosulfate.. Thiocyanate Reduced Operating Costs with the MAX- Capillary Format The MAX- Capillary column (.5 5 mm) offers the same selectivity as the MAX- analyticalscale column, thus producing the same performance as a mm column, but requiring only / th the eluent flow rate. The capillary format offers the advantage of less eluent consumption, providing reduced operating costs. Figure 5 illustrates the separation of organic acids and inorganic anions using the MAX- Capillary column. System Recommendations The MAX- ( mm) column in the suppressed conductivity mode is recommended for use with the ICS- or ICS-5 IC systems equipped with an EG. The MAX- ( mm) column can be used with older Dionex IC Systems equipped with an EG or an RFC- Reagent-Free Controller. The EG is used to automatically produce hydroxide gradients from deionized water. The MAX- Capillary column is recommended for use with the ICS-5 Capillary scale system. Figure. Separation of common petrochemical anions using the IonSwift MAX- analytical column. Suppressors For optimum ease of use and economy, the MAX- ( mm) column can be used with the ASRS ( mm) Anion Suppressor. For the MAX- column (.5 mm), use the ACES suppressor. Anion Trap Columns When using the EG for eluent delivery, a CR-ATC Continuously Regenerated Anion Trap Column should be installed between the EG cartridge and the EG degas module. Alternatively, for the mm MAX- column, an ATC-HC Anion Trap Column can be installed between the pump outlet and inlet of the EG cartridge in the EG module. Concentrator Columns For concentrator work with the MAX- ( mm), use the IonSwift MAX- guard column, ultratrace anion concentrator columns (UTAC- LP, UTAC-ULP, UTAC-XLP, UTAC-LP, UTAC-ULP, UTAC- XLP), or trace anion concentrator column (TAC-ULP, or TAC-) when a single piston pump such as the AXP pump (pulse damper required) is used for sample delivery. Use the UTAC- LP, UTAC-LP or TAC-LP trace anion concentrator columns when the sample is delivered with a syringe or with a low-pressure autosampler such as the AS-DV.

5 SPECIFICATIONS Dimensions: IonSwift MAX- Analytical 5 mm IonSwift MAX-G Guard 5 mm IonSwift MAX- Capillary.5 5 mm IonSwift MAX-G Capillary Guard.5 5 mm Maximum Operating Pressure: psi Mobile Phase Compatibility: ph ; % HPLC solvents Substrate Characteristics: Analytical and Guard Monolithic Backbone Modal Pore Size:. µm (Mercury Porosimetry) Crosslinking (%DVB): 55% Ion Exchange Group: Functional Group: Alkanol quaternary ammonium ion Functional Group Characteristics: Hydrophobicity: Medium low hydrophobic Capacity: µeq ( 5 mm column). µeq ( 5 mm column). µeq (.5 5 mm column). µeq (.5 5 mm column) Column Construction: PEEK with threaded ferrule-style end fittings. All components are nonmetallic. IonSwift MAX-G, Peaks: MAX- Capillary column Eluent:. mm mm KOH in 5 min, mm 5 mm in 5 min, 5 mm 5 mm KOH in min Eluent Source: EGC KOH capillary Flow Rate: µl/min Inj. Volume:. µl Detection: Suppressed conductivity, ACES AutoSuppression recycle mode 5 9. Fluoride. mg/l. Acetate. Formate. Butyrate 5. Galacturonate. Chloride. Nitrite. Bromide 9. Nitrate Figure 5. Separation of organic acids and inorganic anions using the IonSwift MAX- capillary column. 5. Carbonate. Sulfate. Oxalate. Tungstate. Phosphate 5. Chromate. Citrate. Isocitrate. cis-aconitate 9. trans-aconitate 9 5

6 In the U.S., call () -9 or contact the Dionex Regional Office nearest you. Outside the U.S., order through your local Dionex office or distributor. Refer to the following part numbers: IonSwift MAX- Columns IonSwift MAX- Analytical Column ( 5 mm)... 9 IonSwift MAX-G Guard Column ( 5 mm)... IonSwift MAX- Capillary Column (.5 5 mm)... IonSwift MAX-G Capillary Guard Column (.5 5 mm)... Trap Columns CR-ATC Continuously Regenerated Anion Trap Column (For use with systems equipped with an Eluent Generator or RFC-)... CR-ATC Continuously Regenerated Anion Trap Column (Capillary) (For use with Capillary Anions Columns)... Concentrator Columns UTAC-LP Ultra Trace Anion Concentrator-Low Pressure ( 5 mm)... 9 UTAC-ULP Ultra Trace Anion Concentrator-Ultra Low Pressure (5 mm)... 5 UTAC-XLP Ultra Trace Anion Concentrator-Extremely Low Pressure ( mm) UTAC-LP Ultra Trace Anion Concentrator-Low Pressure ( 5 mm)... 9 UTAC-ULP Ultra Trace Anion Concentrator-Ultra Low Pressure (5 mm)... UTAC-XLP Ultra Trace Anion Concentrator-Extremely Low Pressure ( mm)... Suppressors ORDERING INFORMATION ASRS ( mm) Anion Self-Regenerating Suppressor ACES Anion Capillary Electrolytic Suppressor... 5 (For use with the anion capillary columns) ACES, IonSwift, and Reagent-Free are trademarks and ASRS, AutoSuppression, and IonPac are registered trademarks of Dionex Corporation. PEEK is a trademark of Victrex PLC. Dionex Corporation Titan Way P.O. Box Sunnyvale, CA North America U.S./Canada () 95-5 South America Europe Austria () 5 5 Benelux () 9 () 5 9 Denmark (5) 9 9 France () 9 Germany (9) 99 Ireland (5) Italy (9) 5 Sweden () Asia Pacific Australia () 9 5 China (5) India (9) 5 Japan () 5 Korea () 5 5 Singapore (5) 9 9 Taiwan () 5 55 Brazil (55) 5 LPN 5- M / 9- Switzerland () 5 99 United Kingdom () 9 () - Dionex Corporation