Achieve FASTERLC. with Onyx Monolithic Silica HPLC Columns. Featuring: New Onyx HD Columns for Increased Efficiency!

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1 Achieve FASTERLC with Onyx Monolithic Silica HPLC Columns Reduce run times Increase throughput Rapidly screen dirty or complex samples Featuring: New Onyx HD Columns for Increased Efficiency!

2 Onyx Monolithic Silica HPLC Columns Designed for high speed analysis, the monolithic nature of Onyx columns allows for dilute-and-shoot applications, saving scientists valuable sample preparation time. Onyx columns feature a dense network of macropores that allows viscous samples and mobile phase to flow through the column less restricted than compared to particle-based columns. Monolithic Technology vs. Particle-Based Technology Onyx: Monolithic porous silica rod Significantly shorter run times Cut methods by more than half Low backpressures Less stress on system and column High flow rates Due to high porosity No inlet bed settling Increased reliability, reproducibility, and lifetime Particle-Based Columns: Individual silica particles High flow resistance Limits ability to shorten run times Increased backpressure Limits life of pumps, seals, and column Reduced throughput Long run times Bed splitting possible Shortens column life & lessens reproducibility Interested in learning about other Phenomenex HPLC columns and their benefits? Visit ColumnMatch.com A reversed phase column screening application that instantly sorts through the available selectivities and finds the right columns for your application. Visit to find the perfect column for your needs. Phenomenex l WEB:

3 Increase Sample Throughput and Reduce Analysis Time Cut run times by more than half! Results in /4 the time! ml/min (30 bar) App ID min min App ID 55 ml/min (6 bar) 0 3 min 3 ml/min (95 bar) 0 3 min App ID 5507 App ID ml/min (30 bar) 0 3 min App ID 55 ADME / DMPK Wide macropores reduce intereference from proteins allowing samples to easily pass through Monolithic structure enables rapid gradients with very short re-equilbration times, resulting in methods less than 4 min Improved flow characteristics of monoliths results in lower sample carryover for complex matrices like urine plasma Food and Beverage Flow restrictions and overpressures, due to salts, precipitated proteins, and lipids in the sample matrix, are highly unlikely Increase resolving power of very complex food extracts by column coupling Analyze very dilute or low-level analytes by a direct, high-flow injection onto the column Intens. x0 5 mau AppID AppID min min Sample Prep: Human urine sample diluted : with water. 50 μl injected Column: Onyx Monolithic C Dimensions: 00 x 4.6 mm Part No.: CH Mobile Phase: A: 0. % Formic acid in Water B: 0. % Formic acid in Acetonitrile Gradient: 5-90 % B in 4.0 min Flow Rate: 4.0 ml/min Temperature: 30 C Detection: LC/MS ESI + (ambient) Injection:. Nordiazepam (Diazepam metabolite) (m/z = 7). Diazepam (m/z = 5) Column: Onyx Monolithic C Dimensions: 00 x 4.6 mm ( - 00 x 4.6 mm columns coupled in series) Part No.: CH Mobile Phase: A: 0. % TFA in Water B: 0.0 % TFA in Acetonitrile Gradient: 5-70 % B in 5 minutes Flow Rate:.0 ml/min Temperature: 30 C Detection: 0 nm Sample: Multi-grain cereal

4 5 4 3 Onyx HD A Higher Efficiency Monolithic Column Increased Resolution of Steroids with Onyx HD-C Onyx C Onyx HD-C Higher Resolution! ,0 3, App ID 9994 App ID min min Conditions for all columns: Column: Onyx C Onyx HD-C Dimension: 00 x 4.6 mm Mobile Phase: A: Water B: Methanol Gradient: Time (min) % B Flow Rate:. ml/min Temperature: 5 C Detection: 30 nm Sample:. Triamcinolone. Esteriol 3. Prednisone 4. Hydrocortisone 5. Cortisone 6. Corticosterone 7. -α-hydroxyprogesterone. ß-Estradiol 9. -α-hydroxyprogesterone 0. α-estradiol. 7-α-Hydroxyprogesterone. Deoxycorticosterone 3. Progesterone 4. Betamethasone Valerate Increased Performance with Onyx HD-C Excellent Batch-to-Batch and Column-to-Column Reproducibility Onyx HD-C, 00 x.0 mm Onyx C, 00 x.0 mm Plate H (µm) 0 mau Linear Velocity (mm/s) min Column: Onyx HD-C Dimension: 00 x 4.6 mm Part No.: CH0-6 Mobile Phase: A: Acetonitrile B: 0 mm NaH PO 4 Buffer (ph 7.6) Gradient: Time (min) % B Flow Rate:. ml/min Temperature: Ambient Detection: 54 nm Sample:. Caffeine. Aniline 3. N-Methylaniline 4. -Ethylaniline 5. 4-Nitranisole 6. N,N-Dimethylaniline Phenomenex l WEB:

5 Ordering Information Material Characteristics Macropore Size (μm) Mesopore Size (Å) Pore Volume (ml/g) Surface Area (m /g) Carbon Load % Calculated Bonded Phase Coverage End (µmole/m ) Capping Packing Material Onyx Silica No Onyx C Yes Onyx C Yes Onyx C* Yes Onyx HD-C Yes Maximum Pressure: 00 Bar; ph Range: *50 x.0 mm ID only; enhanced.5 μm macropre size for higher efficiencies If Onyx analytical columns do not provide at least an equivalent separation as compared to a competing column of the same monolithic characteristics, similar phase, and dimensions, return the column with comparative data within 45 days for a FULL REFUND. Ordering Information Part No. Description Size (mm) Price Capillary Columns CH0-3 Onyx Monolithic C 50 x 0.05 CH Onyx Monolithic C 50 x 0. CH0-39 Onyx Monolithic HD-C 50 x 0. CH0-390 Onyx Monolithic C 50 x 0. CH0-39 Onyx Monolithic HD-C 50 x 0. CH0-39 Onyx Monolithic C Trapping Column 50 x 0. CH0-393 Onyx Monolithic C 50 x 0. Analytical Columns CH0-373 Onyx Monolithic C 50 x.0 CH0-467 Onyx Monolithic C 00 x.0 CH0-464 Onyx Monolithic C 5 x 3.0 CH0-463 Onyx Monolithic C 50 x 3.0 CH0-5 Onyx Monolithic C 00 x 3.0 CH Onyx Monolithic C 00 x 4.6 CH Onyx Monolithic C 50 x 4.6 CH Onyx Monolithic C 5 x 4.6 CH0-6 Onyx Monolithic HD-C 00 x 4.6 CH0-6 Onyx Monolithic HD-C 50 x 4.6 CH0-63 Onyx Monolithic HD-C 5 x 4.6 CH Onyx Monolithic C 00 x 4.6 CH0-764 Onyx Monolithic Si 00 x 4.6 SemiPrep Columns CH0-77 Onyx Monolithic C 00 x 0.0 Guard Cartridge System KJ0-46 Onyx Monolithic C Guard Cartridge Kit (3/pk cartridges + holder) 5 x.0 CH0-469 Onyx Monolithic C Guard Cartridges (3/pk) 5 x.0 KJ0-465 Onyx Monolithic C Guard Cartridge Kit (3/pk cartridges + holder) 5 x 3.0 CH0-466 Onyx Monolithic C Guard Cartridges (3/pk) 5 x 3.0 KJ0-765 Onyx Monolithic C Guard Cartridge Kit (3/pk cartridges + holder + wrench) 5 x 4.6 CH Onyx Monolithic C Guard Cartridges (3/pk) 5 x 4.6 KJ0-65 Onyx Monolithic HD-C Guard Cartridge Kit 5 x 4.6 (3/pk cartridges + holder + wrench) CH0-66 Onyx Monolithic HD-C Guard Cartridge (3/pk) 5 x 4.6 KJ0-765 Onyx Monolithic C Guard Cartridge Kit (3/pk cartridges + holder + wrench) 0 x 4.6 CH Onyx Monolithic C Guard Cartridges (3/pk) 0 x 4.6 Method Validation Kit KH Onyx Monolithic C Method Validation Kit (3 columns from different batches) 00 x 4.6 Column Coupler AQ Onyx Column Coupler, 0.00 in. ID Phenomenex l WEB:

6 Australia t: f: au Austria t: f: Belgium t: (French) t: (Dutch) f: +3 (0) be Canada t: (00) f: (30) Denmark t: f: nordic Finland t: f: nordic France t: f: france Germany t: f: anfrage@phenomenex.com India t: f: india Ireland t: f: eire Italy t: f: italia Luxembourg t: +3 (0) f: +3 (0) nl Mexico t: f: tecnicomx@phenomenex.com The Netherlands t: f: nl New Zealand t: f: nz Norway t: f: nordic Puerto Rico t: (00) 54-HPLC f: (30) Sweden t: f: nordic United Kingdom t: f: uk United States t: (30) f: (30) All other countries: Corporate Office USA t: (30) f: (30) Phenomenex products are available worldwide. For the distributor in your country, contact Phenomenex USA, International Department at international@phenomenex.com Terms and Conditions Subject to Phenomenex Standard Terms and Conditions, which may be viewed at Trademarks Onyx and ColumnMatch.com are trademarks of Phenomenex. Disclaimer Comparative separations may not be representative of all applications. Onyx is a Phenomenex product based on monolithic technology under license from Merck KGaA, Darmstadt, Germany. 03 Phenomenex, Inc. All rights reserved. BR97703_W