Haematology/Blood Transfusion. Uncertainty of Measurement values. Haemoglobin g/l / g/l

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1 Haematology/Blood Transfusion Uncertainty of Measurement values Full Blood Count WBC < /L / /L WBC /L / /L WBC > /L / /L RBC /L / /L Haemoglobin g/l / g/l Haemoglobin <80 g/l / g/l Haemoglobin >190 g/l / g/l Haematocrit L/L / L/L Haematocrit >0.56 L/L / L/L MCV fl / fl MCV <75 fl / fl MCV fl / fl MCHC >105 g/l / g/l MCHC >370 g/l / g/l Platelets /L / /L Platelets < /L / /L Neutrophils /L / /L Neutrophils < /L / /L Lymphocytes /L / /L Lymphocyte > /L / /L Monocytes /L / /L Eosinophils /L / /L Basophils /L / /L

2 Coagulation Normal PT secs / secs Raised PT > 30 secs / secs Normal INR ratio / INR ratio / INR ratio / INR >5 >5.0 ratio / Normal APTT (Actin FS) secs / secs Raise APTT (Actin FS) > 50 secs / secs Raise APTT (Actin FS) > 40 secs / secs APTT (synthasil) secs / secs Raised APTT (synthasil) >40 secs / secs Fbg low g/l / g/l Fbg norm g/l / g/l Thrombin Time secs / secs Thrombin Time > 20 secs / D-Dimer norm Ug/mIFEU / Ug/mIFEU Raised D Dimer > 0.5 Ug/mIFEU / Ug/mIFEU Reptilase secs / secs Erythrocyte Sedimentation Rate Gateshead Autocompact Normal ESR 1-10 mm / mm Raised ESR mm / mm Gateshead Interrliner Normal ESR 1-10 mm / mm 0.12 Raised ESR mm / mm 0.234

3 South Tyneside Autocompact Normal ESR 1-10 mm / mm Raised ESR mm / mm Sunderland Interrliner Normal ESR 1-10 mm /- mm Raised ESR mm /- mm Rapid Diagnostic Testing Malaria The laboratory has found the value of an uncertainty of measurement of 0.57% probability that a Rapid Diagnostic Test for Malaria is misinterpreted by a Biomedical Scientist within the department. critical limit Negative, Positive (P.Falciparum) Positive (P.Vivax/ Ovale/ Malarie) Positive (P.Falciparum + Mixed Infection) Expected range/ critical limit Case dependent Digital Morphology DI-60 Measurement based on % comparability with cell count. 100% > 97% / % Digital Morphology DM-96 (QEH site) 100% > 97% / %

4 Digital Morphology DM-96 (SRH site) 100% > 97% /-1.02 % Digital Morphology DM-1200 (STH site) 100% > 97% /- 0.45% Infectious Mononucleosis The laboratory has found the value of an uncertainty of measurement of 0.0 % probability that a Rapid Diagnostic Test for Malaria is misinterpreted by a Biomedical Scientist within the department. Positive/ Negative As expected Sebia Minicap Flex Hb A % / % Hb A % / % HbA % / % HbF % / % HbS % / % HbC % / % Group and Antibody Screen The laboratory has found the value of an uncertainty of measurement of 0.0 % for performance of group and antibody screen on the IH1000. All results and grade of reaction strength were as expected during verification studies. Crossmatch

5 The laboratory has found the value of an uncertainty of measurement of 0.0 % for inter-laboratory crossmatch. Kleihauer Mean SD Uncertainty FMH 2ml 2ml /-0.96 mls FMH 4ml 4ml / mls FMH>12 >12ml / mls 0.377