MALDI Biotyper Troubleshooting Guide

Transcription

1 Serial Number: MALDI Biotyper Troubleshooting Guide Revision B

2 Calibration/Validation Issues If failed Validation, run Manual Calibration using flexcontrol software in RUO mode (shown in image below) Check the expiration dates of the Standard Solvent (formerly known as Organic Solvent), BTS and Matrix Ensure proper preparation of reagents utilizing Bruker's Instructions for Use Spot newly prepared BTS and Matrix Perform a Source Cleaning (if using a microflex LT/SH) If issues persist - Call Service to request a routine optimization

3 Vacuum Issues If in CA mode, run will abort. Log onto RUO mode and check vacuum pressure Vacuum Pressure Go to flexcontrol>status tab>details>vacuum tab>gauges> Source High>Pressure (red light indicates a failure) RED LIGHT: Eject the target to OUT position Open the lid and remove all debris by cleaning the O-ring area, the black O-ring, and the lid with a lint-free Kim Wipe (do NOT use alcohol or any other chemicals!)

4 Performing an Automated Ion Source Cleaning Start an automated Source Cleaning to clean Ion Source (recommended weekly) flexcontrol>status tab>details>processor Subsystem tab>service Information>Start NOTE: Target may be in IN or OUT position to begin the cleaning Wait until Source Cleaning is complete (~20 minutes); once complete a message will appear on the screen If a Source Cleaning error message appears check your vacuum HINT: See Vacuum Issues

5 "Error Call Service" Message Before contacting Service, try the following steps: Close MBT CA Software Close flexcontrol Software (If logged in as TOF-User) Re-Open flexcontrol Software (If logged in as TOF-User) Re-Open MBT CA Software Re-Start the Run

6 Reboot the Microflex May be necessary in order to re-establish PC connection or to fix software freezes Close all Bruker software windows Shut down the PC using "Start" menu => "Shutdown" Turn the key in the front of the instrument to vertical position OR toggle the orange switch in the back Wait 15 seconds or until all of the lights on the front panel have turned OFF Turn the key to horizontal position or toggle the orange switch and wait until all the lights on the front panel stop flashing Turn on PC using power button located on the PC tower Re-launch Bruker software

7 Shut Down the Microflex May be necessary in order to prepare for a scheduled power outage at your facility Close all Bruker software windows Shut down the PC using "Start" menu => "Shutdown" Turn the key in the front of the instrument to vertical position OR toggle the orange switch in the back Once power is restored, turn the key to horizontal position or toggle the orange switch and wait until all the lights on the front panel stop flashing Turn on PC using power button located on the PC tower HINT: Time to restore vacuum is dependent on how long the system was shut down

8 Software Freeze Try to close the Biotyper software and flexcontrol software by clicking the X on the top right corner and then reopen the programs If the software is still frozen open Task Manager HINT: Move mouse to the bottom of the screen and right click OR press Ctrl+Alt+Delete. Select Start Task Manager Select the Processes tab Close the following programs (this will depend on what programs are currently open and frozen) and then reopen the programs BiotyperRTC.exe / BiotyperCA.exe (depending on mode) TOFControl.exe FlexHal.exe

9 Local Host Error Contact your IT department and ask them to unblock Port 8080, 8180, 8280 and on the network s Firewall Problem persists - Call Service

10 WebEx Setup Open Internet Explorer Go to bruker.webex.com (do not enter If website is NOT reached, contact IT department to check internet connection Answer the Preference questions according to your time and location Enter the meeting number you receive from your Support Engineer and click JOIN HINT: save to Favorites

11 Misaligned Spot Position Reteach the target flexcontrol>sample Carrier tab>click Advanced>click Teach Click Go for First position By clicking the mouse in the camera view, center the cross hair on the target spot Continue to Second Position and Third Position Click Save as Default Teaching and OK

12 High Temperature Error Close all the Bruker software on the PC Reboot the microflex HINT: See "Reboot the microflex" Problem persists- Call Service

13 Detector Check Message Clear the message by pressing "Clear"

14 UPS Blinking Lights or Beeping Check main power to UPS Note: UPS required 110V Make sure ONLY the Computer and the microflex are plugged into the UPS The Printer should NOT be plugged into the UPS

15 Bruker Daltonics Inc. Biotyper Hotline: Access code: 724 (after hours only) ms.support.us@bruker.com