VertiSep TM GES. HPLC Columns. VertiSep TM GES HPLC Columns
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1 High purity Silica offer high stability, efficiency, reproducibility Moderate pore size and surface area are perfect for general purpose High carbon loading C18 offers high degree of hydrophobicity for high resolution of organic compounds that have similar structures Fully endcapped to improve peak symmetry Polymeric bonding offers high stability and longer column lifetime Economic Price packings are high purity Silica which contains less amounts of metal ions and sulfate contaminants offer high stability at ph *, efficiency, column-to-column reproducibility. packings are moderate pore size and surface area proving general purpose separations of variety of compounds. reverse phase C18 packings are high carbon loading of 17% offerring the high degree of hydrophobicity for high resolution of organic compounds that have similar structures such as Polynuclear Aromatic Hydrocarbons, Benzidines, Vitamins D2, D3, Amino acids. reverse phase packings are fully endcapped an show selectivity as conventional C18 phases. packings are polymeric bonding resulting high stability and longer column lifetime. are manufactured by statistic process control of silica synthesis, bonding and column packing. The reproducible column packing method control provides exceptional efficiency, symmetry and reproducible capacity factor. Specifications Packing Carbon Pore Pore Surface End Load Volume Area Capped (mm) (%) (Å) (ml/g) (m 2 /g) C18 3,5, Yes C8 3,5, Yes Ph 3,5, Yes C4 3,5, Yes CN 3,5, Yes NH2 3,5, No Si 3,5, No SAX No SCX No Reproducible Column Packing Method Controls 54 * CN is stable at ph packing Test Conditions C18, 5μm, 4.6x250mm 60:40 Acetonitrile/Water Detector: Sample: Reversed phase test mix
2 Aromatic Bases Aromatic Acid 1. Aniline 2. Dimethylaniline 3. Diethylaniline 1. Homovanilic Acid 2. Benzoic Acid 3. Salicylic Acid C18 5μm 150x4.6mm Acetonitrile:Water:TEA (65:34:1) Alkyl Bensoic Acids 1. 4-Butylbenzoic Acid 2. 3,5-Di-tert-Butylbenzoic Acid 3. 4-Hexylbenzoic Acid 4. 4-Octylbenzoic Acid C18 5μm 150x4.6mm 0.05M KH 2 (ph3.2):methanol (60:40) Steroids 1. Hydrocortisone 2. Testosterone 3. Progesterone C18 5μm 150x4.6mm Acetonitrile:Water:Acetic (65:34:1) C18 5μm 150x4.6mm Methanol:Water (70:30) Steroids Acids and Bases 1. Cortisone Degradant 2. Hydrocortisone-21- acetate Degradant 3. Cortisone Degradant 4. Hydrocortisone-21- acetate Degradant 5. Cortisone 6. Hydrocortisone-21- acetate 1. N,N-Diethylaniline 2. Phenylacetic Acid 3. N,N-Dimethylaniline 4. Phenylvaleric Acid C18 5μm 150x4.6mm 50mM KH 2 (ph2.75):acetonitrile (65:35) UV252nm C18 5μm 150x4.6mm Acetonitrile:0.05M Potassium Phosphate Buffer (ph3.0), (45:55) 55
3 Aflatoxins Aromatic Ketone 1. Aflatoxin G2 0.3μg/mL 2. Aflatoxin G1 1.0μg/mL 3. Aflatoxin B2 0.3μg/mL 4. Aflatoxin B1 1.0μg/mL 1. 1-hydroxy-7-azabenzotriazole 2. 4-methylbenzene sulfonamide 3. methyl 3-amino-2- thiophenecarboxylate 4. 4-aminobenzophenone Gradient: C18 5μm 4.6x250mm Water:Acetonitrile:Methanol (60:20:20) UV365nm Coenzyme A 1. Coenzyme A 2. (R)-3-Hydroxybutyryl-Coenzyme A 3. Crotonoyl-Coenzyme A C18 5μm 4.6x250mm A: Methanol:10mM KH 2 (ph6.5), (0:100) B: Methanol:10mM KH 2 (ph6.5), (40:60) Time/%B: 0/30, 10/80 0.8mL/min UV260nm Gradient: C18 5μm 4.6x100mm A: 0.1%TFA in water B: 0.08%TFA in acetonitrile Time:%B: 0:0, 4:60 FAMEs 1. C8 2. C10 3. C12:0 + C18:3 5. C14:0 6. C18:1 8. C18:0 C18 5μm 4.6x250mm Acetonitrile:Acetone (60:40) R401 refractometer Furosine Isocyanate Derivatives Mixture 1. furosine 1. ACN/DMSO 2. 2,6-TDI 3. HDI 4. 2,4-TDI 5. MDI C8 5μm 4.6x250mm 0.06M sodium acetate (ph 4.3) with acetic acid 2.0mL/min UV280nm, 0.1 AUFS CN 5μm 4.6x250mm 0.05M Ammonium acetate in Water:Acetonitrile (70:30), (ph 6.0) 0 min - 1mL/min 15 min - 2mL/min Fluorescence, 240nm excitation, 370nm emission 56
4 Nucleotides 1. ATP 2. ADP 3. AMP 4. NADP 5. NAD Saccharide 1. Fructose 2. Glucose 3. Sucrose 4. Maltose 5. Lactose Gradient: C18 5μm 250x4.6mm A: 0.1M KH 2 (ph 6) B: A:Methanol (90:10) Time:B: 0:0, 15:25, 17.5:90, 19:100, 24:0 1.3mL/min Patulin in Apple Juice 1. Patulin NH2 5μm 250x4.6mm Acetonitrile:Water (80:20) 1.5mL/min RI Porphyrin Isomers 1. Uroporphyrin I 2. Uroporphyrin III 3. Coproporphyrin I 4. Coproporphyrin III C18 5μm 250x4.6mm Acetonitrile:Water (5:95) 1.5mL/min UV275nm C18 3μm 250x4.6mm Methanol:1M Ammonium acetate (ph 5.2) 2.0mL/min UV406nm Salicylic Acid White Wine 1. 4-Hydroxybenzoic Acid 2. 4-Hydroxyisophthalic Acid 3. Phenol 4. Salicylic Acid 1. Maleic acid 2. Malic acid 3. Tartaric acid 4. Fumaric acid C18 5μm 100x4.6mm Methanol:Water:Acetic acid (40:59:1) 0.5mL/min UV270nm SAX 5μm 250x4.6mm Acetonitrile:50mM KH2PO4 (ph 5.50), (35:65) UV210nm 57
5 Alkyl Benzoic Acids Base/Neutral Mix 1. 4-Butylbenzoic Acid 2. 3,5,-di-tert-Butylbenzoic Acid 3. 4-Hexylbenzoic Acid 4. 4-Octylbenzoic Acid 1. Uracil 2. Phenol 3. Benzaldehyde 4. N,N-Diethyl-m-toluamide 5. Toluene 6. Ethylbenzene C8 5μm 4.6x150mm Acetonitrile:Water:Acetic Acid (65:34:1) Carbohydrates from Wine 1. Ethanol 2. Glucose 3. Sucrose NH2 10μm 4.6x250mm Acetonitrile:Water (75:25) 3.0mL/min RI Gradient: C18 5μm 4.6x250mm Acetonitrile:Water (65:35) Nucleotides 1. CMP 2. AMP 3. UMP 4. GMP 5. CDP 6. ADP 7. UDP 8. CTP 9. GDP 10. ATP 11. UTP 12. GTP SAX 5μm 4.6x250mm A: 5mM NH 4 H 2 (ph2.8) B: 750mM NH 4 H 2 (ph 3.7) Time:%B: 0:0, 3:100 Aromatic Hydrocarbons Aromatic Ketones 1. Toluene 2. Nitrobenzene 3. 2,6-Dinitrotoluene 1. Benzamide 2. Benzyl Alcohol 3. Acetophenone 4. Methyl Benzoate 5. Phenetole 6. Naphthalene 7. Benzophenone 8. Biphenyl CN 5μm 4.6x100mm 1% Ethyl Acetate in Hexane 2.0mL/min PH 5μm 4.6x100mm Methanol:Water (60:40) 58
6 C18 3 Ultra-Fast 2.1 x AA-B111 3 Ultra-Fast 2.1 x AA-B611 3 Ultra-Fast 2.1 x AA-B211 3 LC/MS 2.1 x AA-B311 3 LC/MS 2.1 x AA-B411 3 LC/MS 3.2 x AA-C311 3 LC/MS 3.2 x AA-C411 3 Hi-Speed 4.6 x AA-E211 3 Analytical 4.6 x AA-E311 3 Analytical 4.6 x AA-E411 3 Hi-Speed 7.8 x AA-G211 3 Analytical 7.8 x AA-G311 5 LC/MS 2.1 x AA-B321 5 LC/MS 2.1 x AA-B421 5 LC/MS 2.1 x AA-B521 5 LC/MS 3.2 x AA-C321 5 LC/MS 3.2 x AA-C421 5 LC/MS 3.2 x AA-C521 5 Hi-Speed 4.6 x AA-E221 5 Analytical 4.6 x AA-E321 5 Analytical 4.6 x AA-E421 5 Analytical 4.6 x AA-E521 5 Prep 10.0 x AA-H421 5 Prep 10.0 x AA-H521 5 Prep 21.2 x AA-I421 5 Prep 21.2 x AA-I Analytical 4.6 x AA-E Prep 10.0 x AA-H Prep 10.0 x AA-H Prep 21.2 x AA-I Prep 21.2 x AA-I Prep 30.0 x AA-J Prep 30.0 x AA-J Prep 50.0 x AA-K Prep 50.0 x AA-K531 C8 3 Ultra-Fast 2.1 x AB-B111 3 Ultra-Fast 2.1 x AB-B611 3 Ultra-Fast 2.1 x AB-B211 3 LC/MS 2.1 x AB-B311 3 LC/MS 2.1 x AB-B411 3 LC/MS 3.2 x AB-C311 3 LC/MS 3.2 x AB-C411 3 Hi-Speed 4.6 x AB-E211 3 Analytical 4.6 x AB-E311 3 Analytical 4.6 x AB-E411 3 Hi-Speed 7.8 x AB-G211 3 Analytical 7.8 x AB-G311 5 LC/MS 2.1 x AB-B321 5 LC/MS 2.1 x AB-B421 5 LC/MS 2.1 x AB-B521 5 LC/MS 3.2 x AB-C321 5 LC/MS 3.2 x AB-C421 5 LC/MS 3.2 x AB-C521 5 Hi-Speed 4.6 x AB-E221 5 Analytical 4.6 x AB-E321 5 Analytical 4.6 x AB-E421 5 Analytical 4.6 x AB-E521 5 Prep 10.0 x AB-H421 5 Prep 10.0 x AB-H521 5 Prep 21.2 x AB-I421 5 Prep 21.2 x AB-I Analytical 4.6 x AB-E Prep 10.0 x AB-H Prep 10.0 x AB-H Prep 21.2 x AB-I Prep 21.2 x AB-I Prep 30.0 x AB-J Prep 30.0 x AB-J Prep 50.0 x AB-K Prep 50.0 x AB-K531 59
7 Ve rtisep TM GES Ph 3 Ultra-Fast 2.1 x AD-B111 3 Ultra-Fast 2.1 x AD-B611 3 Ultra-Fast 2.1 x AD-B211 3 LC/MS 2.1 x AD-B311 3 LC/MS 2.1 x AD-B411 3 LC/MS 3.2 x AD-C311 3 LC/MS 3.2 x AD-C411 3 Hi-Speed 4.6 x AD-E211 3 Analytical 4.6 x AD-E311 3 Analytical 4.6 x AD-E411 3 Hi-Speed 7.8 x AD-G211 3 Analytical 7.8 x AD-G311 5 LC/MS 2.1 x AD-B321 5 LC/MS 2.1 x AD-B421 5 LC/MS 2.1 x AD-B521 5 LC/MS 3.2 x AD-C321 5 LC/MS 3.2 x AD-C421 5 LC/MS 3.2 x AD-C521 5 Hi-Speed 4.6 x AD-E221 5 Analytical 4.6 x AD-E321 5 Analytical 4.6 x AD-E421 5 Analytical 4.6 x AD-E521 5 Prep 10.0 x AD-H421 5 Prep 10.0 x AD-H521 5 Prep 21.2 x AD-I421 5 Prep 21.2 x AD-I Analytical 4.6 x AD-E Prep 10.0 x AD-H Prep 10.0 x AD-H Prep 21.2 x AD-I Prep 21.2 x AD-I Prep 30.0 x AD-J Prep 30.0 x AD-J Prep 50.0 x AD-K Prep 50.0 x AD-K531 C4 3 Ultra-Fast 2.1 x AC-B111 3 Ultra-Fast 2.1 x AC-B611 3 Ultra-Fast 2.1 x AC-B211 3 LC/MS 2.1 x AC-B311 3 LC/MS 2.1 x AC-B411 3 LC/MS 3.2 x AC-C311 3 LC/MS 3.2 x AC-C411 3 Hi-Speed 4.6 x AC-E211 3 Analytical 4.6 x AC-E311 3 Analytical 4.6 x AC-E411 3 Hi-Speed 7.8 x AC-G211 3 Analytical 7.8 x AC-G311 5 LC/MS 2.1 x AC-B321 5 LC/MS 2.1 x AC-B421 5 LC/MS 2.1 x AC-B521 5 LC/MS 3.2 x AC-C321 5 LC/MS 3.2 x AC-C421 5 LC/MS 3.2 x AC-C521 5 Hi-Speed 4.6 x AC-E221 5 Analytical 4.6 x AC-E321 5 Analytical 4.6 x AC-E421 5 Analytical 4.6 x AC-E521 5 Prep 10.0 x AC-H421 5 Prep 10.0 x AC-H521 5 Prep 21.2 x AC-I421 5 Prep 21.2 x AC-I Analytical 4.6 x AC-E Prep 10.0 x AC-H Prep 10.0 x AC-H Prep 21.2 x AC-I Prep 21.2 x AC-I Prep 30.0 x AC-J Prep 30.0 x AC-J Prep 50.0 x AC-K Prep 50.0 x AC-K531 60
8 CN 3 Ultra-Fast 2.1 x AE-B111 3 Ultra-Fast 2.1 x AE-B611 3 Ultra-Fast 2.1 x AE-B211 3 LC/MS 2.1 x AE-B311 3 LC/MS 2.1 x AE-B411 3 LC/MS 3.2 x AE-C311 3 LC/MS 3.2 x AE-C411 3 Hi-Speed 4.6 x AE-E211 3 Analytical 4.6 x AE-E311 3 Analytical 4.6 x AE-E411 3 Hi-Speed 7.8 x AE-G211 3 Analytical 7.8 x AE-G311 5 LC/MS 2.1 x AE-B321 5 LC/MS 2.1 x AE-B421 5 LC/MS 2.1 x AE-B521 5 LC/MS 3.2 x AE-C321 5 LC/MS 3.2 x AE-C421 5 LC/MS 3.2 x AE-C521 5 Hi-Speed 4.6 x AE-E221 5 Analytical 4.6 x AE-E321 5 Analytical 4.6 x AE-E421 5 Analytical 4.6 x AE-E521 5 Prep 10.0 x AE-H421 5 Prep 10.0 x AE-H521 5 Prep 21.2 x AE-I421 5 Prep 21.2 x AE-I Analytical 4.6 x AE-E Prep 10.0 x AE-H Prep 10.0 x AE-H Prep 21.2 x AE-I Prep 21.2 x AE-I Prep 30.0 x AE-J Prep 30.0 x AE-J Prep 50.0 x AE-K Prep 50.0 x AE-K531 NH2 3 Ultra-Fast 2.1 x AF-B111 3 Ultra-Fast 2.1 x AF-B611 3 Ultra-Fast 2.1 x AF-B211 3 LC/MS 2.1 x AF-B311 3 LC/MS 2.1 x AF-B411 3 LC/MS 3.2 x AF-C311 3 LC/MS 3.2 x AF-C411 3 Hi-Speed 4.6 x AF-E211 3 Analytical 4.6 x AF-E311 3 Analytical 4.6 x AF-E411 3 Hi-Speed 7.8 x AF-G211 3 Analytical 7.8 x AF-G311 5 LC/MS 2.1 x AF-B321 5 LC/MS 2.1 x AF-B421 5 LC/MS 2.1 x AF-B521 5 LC/MS 3.2 x AF-C321 5 LC/MS 3.2 x AF-C421 5 LC/MS 3.2 x AF-C521 5 Hi-Speed 4.6 x AF-E221 5 Analytical 4.6 x AF-E321 5 Analytical 4.6 x AF-E421 5 Analytical 4.6 x AF-E521 5 Prep 10.0 x AF-H421 5 Prep 10.0 x AF-H521 5 Prep 21.2 x AF-I421 5 Prep 21.2 x AF-I Analytical 4.6 x AF-E Prep 10.0 x AF-H Prep 10.0 x AF-H Prep 21.2 x AF-I Prep 21.2 x AF-I Prep 30.0 x AF-J Prep 30.0 x AF-J Prep 50.0 x AF-K Prep 50.0 x AF-K531 61
9 Silica 3 Ultra-Fast 2.1 x AG-B111 3 Ultra-Fast 2.1 x AG-B611 3 Ultra-Fast 2.1 x AG-B211 3 LC/MS 2.1 x AG-B311 3 LC/MS 2.1 x AG-B411 3 LC/MS 3.2 x AG-C311 3 LC/MS 3.2 x AG-C411 3 Analytical 4.0 x AG-D311 3 Analytical 4.0 x AG-D411 3 Analytical 4.6 x AG-E311 3 Analytical 4.6 x AG-E411 3 Hi-Speed 7.8 x AG-G211 3 Analytical 7.8 x AG-G311 5 LC/MS 2.1 x AG-B321 5 LC/MS 2.1 x AG-B421 5 LC/MS 3.2 x AG-C321 5 LC/MS 3.2 x AG-C421 5 Analytical 4.0 x AG-D321 5 Analytical 4.0 x AG-D421 5 Analytical 4.0 x AG-D521 5 Analytical 4.6 x AG-E321 5 Analytical 4.6 x AG-EC21 5 Analytical 4.6 x AG-E421 5 Analytical 4.6 x AG-E521 5 Analytical 7.8 x AG-G321 5 Prep 10.0 x AG-H421 5 Prep 10.0 x AG-H521 5 Prep 21.2 x AG-I421 5 Prep 21.2 x AG-I Prep 10.0 x AG-H Prep 10.0 x AG-H Prep 21.2 x AG-I Prep 21.2 x AG-I Prep 30.0 x AG-J Prep 30.0 x AG-J Prep 50.0 x AG-K Prep 50.0 x AG-K531 SCX 5 Analytical 4.6 x AI-E421 5 Analytical 4.6 x AI-E521 SAX 5 Analytical 4.6 x AH-E421 5 Analytical 4.6 x AH-E521 62
10 Guard Cartridge* C18 3 Guard 2.1 x AA-B113 3 Guard 3.2 x AA-C113 3 Guard 4.0 x AA-D113 3 Guard 4.6 x AA-E113 5 Guard 2.1 x AA-B123 5 Guard 3.2 x AA-C123 5 Guard 4.0 x AA-D123 5 Guard 4.6 x AA-E123 5 Guard 10.0 x AA-H123 5 Guard 21.2 x AA-I Guard 10.0 x AA-H Guard 21.2 x AA-I133 C8 3 Guard 2.1 x AB-B113 3 Guard 3.2 x AB-C113 3 Guard 4.0 x AB-D113 3 Guard 4.6 x AB-E113 5 Guard 2.1 x AB-B123 5 Guard 3.2 x AB-C123 5 Guard 4.0 x AB-D123 5 Guard 4.6 x AB-E123 5 Guard 10.0 x AB-H123 5 Guard 21.2 x AB-I Guard 10.0 x AB-H Guard 21.2 x AB-I133 Ph 3 Guard 2.1 x AD-B113 3 Guard 3.2 x AD-C113 3 Guard 4.0 x AD-D113 3 Guard 4.6 x AD-E113 5 Guard 2.1 x AD-B123 5 Guard 3.2 x AD-C123 5 Guard 4.0 x AD-D123 5 Guard 4.6 x AD-E123 5 Guard 10.0 x AD-H123 5 Guard 21.2 x AD-I Guard 10.0 x AD-H Guard 21.2 x AD-I133 C4 3 Guard 2.1 x AC-B113 3 Guard 3.2 x AC-C113 3 Guard 4.0 x AC-D113 3 Guard 4.6 x AC-E113 5 Guard 2.1 x AC-B123 5 Guard 3.2 x AC-C123 5 Guard 4.0 x AC-D123 5 Guard 4.6 x AC-E123 5 Guard 10.0 x AC-H123 5 Guard 21.2 x AC-I Guard 10.0 x AC-H Guard 21.2 x AC-I133 Guard Cartridge* CN 3 Guard 2.1 x AE-B113 3 Guard 3.2 x AE-C113 3 Guard 4.0 x AE-D113 3 Guard 4.6 x AE-E113 5 Guard 2.1 x AE-B123 5 Guard 3.2 x AE-C123 5 Guard 4.0 x AE-D123 5 Guard 4.6 x AE-E123 5 Guard 10.0 x AE-H123 5 Guard 21.2 x AE-I Guard 10.0 x AE-H Guard 21.2 x AE-I133 NH2 3 Guard 2.1 x AF-B113 3 Guard 3.2 x AF-C113 3 Guard 4.0 x AF-D113 3 Guard 4.6 x AF-E113 5 Guard 2.1 x AF-B123 5 Guard 3.2 x AF-C123 5 Guard 4.0 x AF-D123 5 Guard 4.6 x AF-E123 5 Guard 10.0 x AF-H123 5 Guard 21.2 x AF-I Guard 10.0 x AF-H Guard 21.2 x AF-I133 Si 3 Guard 2.1 x AG-B113 3 Guard 3.2 x AG-C113 3 Guard 4.0 x AG-D113 3 Guard 4.6 x AG-E113 5 Guard 2.1 x AG-B123 5 Guard 3.2 x AG-C123 5 Guard 4.0 x AG-D123 5 Guard 4.6 x AG-E123 5 Guard 10.0 x AG-H123 5 Guard 21.2 x AG-I Guard 10.0 x AG-H Guard 21.2 x AG-I133 SCX 5 Guard 4.6 x AI-B113 SAX 5 Guard 4.6 x AI-C113 *Guard holder required Description QTY Part No. Guard Holder with Coupler For column mm For column 10 mm For column 21.2 mm
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