HICHROM. Chromatography Columns and Supplies. HPLC Columns & Materials Partisil & Partisphere. Catalogue 8. Hichrom Limited

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1 HI Chromatography Columns and Supplies & Materials & Partisphere Catalogue 8 Hichrom Limited 1 The Markham Centre, Station Road Theale, Reading, Berks, RG7 4PE, UK Tel: +44 (0) Fax: +44 (0) sales@hichrom.co.uk

2 and Partisphere Acquired by Hichrom from Whatman, a division of GE Healthcare in March 2012 Choice of spherical or irregular silica Wide range of surface chemistries available and Partisphere Acquisition Hichrom acquired the and Partisphere HPLC column range from Whatman, a division of GE Healthcare in March Based in the UK, Hichrom are a leading European manufacturer and distributor of HPLC columns, consumables and media, with manufacturing facilities accredited to both ISO9001 (Quality) and ISO14001 (Environmental) standards. Hichrom have worked closely with GE Healthcare during the manufacture of these products for a number of years, and as such manufacturing protocols, product specifications and part numbers will remain unaffected by the acquisition. Availability The complete range of and Partisphere products is now widely available from Hichrom and our global distributor network. To further assist customers, the majority of GE Healthcare s previous distributors have also been retained, although and Partisphere columns themselves are no longer available directly from GE Healthcare after 16th April To order columns directly or to request details of your nearest distributor, please contact Hichrom by one of the following options: Phone: +44 (0) Fax: +44 (0) sales@hichrom.co.uk Web: The availability of Hichrom manufactured and branded columns remains unaffected by this acquisition. These columns are available in a wider range of dimensions than those previously offered by Whatman/GE Healthcare, and are additionally detailed on pages 173c-173d. Pricing Unchanged for 2012 Hichrom are pleased to confirm that and Partisphere column pricing for 2012 will remain unchanged from the 2012 GE Healthcare US List Price, as detailed on the following pages. All columns are available from stock for immediate delivery. Please contact Hichrom to request availability of any column dimension not listed. s Irregular porous silica µm and 10µm particle sizes Hichrom high efficiency was one of the first commercially available irregular silicas, with a large surface area giving it a high loading capacity. ion exchange materials are widely referenced and remain one of the most popular choices for analysts. ODS3 shows similar performance to Waters µbondapak. Functional Group Particle Size (µm) Silica -,10 C8 Octyl,10 ODS Octadecyl 10 ODS2 Octadecyl 10 ODS3 Octadecyl,10 PAC Amino-cyano,10 SAX Tetramethyl,10 Ammonium SCX Sulphonic Acid, HI LTD

3 3743hic_P abcd:3183hic_P169-p176 27/3/12 09:20 Page 173 and Partisphere Partisphere s Partisphere Spherical µm porous silica Unique void-sealing cartridge hardware Convenient and easy to use - hand tightened fittings Increased column lifetime Functional Group Silica C8 Particle Size (µm) - Octyl C18 Octadecyl PAC Amino-cyano Tetramethyl SAX Ammonium Partisphere was one of the first commercially available spherical silicas, and continues to provide reproducible, high efficiency separations. Partisphere columns are available in a wide range of surface chemistries, including a PFP pentafluorophenyl (TAC-1) column. SCX TAC-1 (PFP) Sulphonic Acid Pentafluorophenyl Proprietary WAX (MAX-1) Partisphere Guard Cartridge System Partisphere columns are available in a unique void sealing (WVS) hardware. If a void eventually forms at the top of the column bed, simply hand tighten the inlet fitting to move the frit assembly downwards and recompress the packed bed, thus removing the void and restoring column efficiency. Large knurled end fittings allow ready hand tightening of the system. All WVS cartridge columns are shipped without end fittings and require end fitting kit p/n this item can be interchanged with additional WVS columns. Additional installation of the unique WVS guard cartridge holder (p/n ) allows the use of guard cartridges (see Figure 1). Figure 1. Partisphere Cartridge System Guard cartridge holder ( ) Unique Void-Sealing Cartridge System End fittings ( ) and Partisphere 2012 International Price List Part Number Description $ USD GBP EUR 80 µm Analytical Columns: µm ODS3 column, 100 x 4.6mm (WCS hardware - RAC-II) µm SAX column, 100 x 4.6mm (WCS hardware - RAC-II) µm SCX column, 100 x 4.6mm (WCS hardware - RAC-II) µm C8 column, 100 x 4.6mm (WCS hardware - RAC-II) µm SIL column, 20 x 4.6mm (WCS hardware) µm PAC column, 20 x 4.6mm (WCS hardware) µm ODS3 column, 20 x 4.6mm (WCS hardware) µm C8 column, 20 x 4.6mm (WCS hardware) µm Analytical Columns: µm SIL column, 20 x 4.6mm (WCS hardware) µm ODS column, 20 x 4.6mm (WCS hardware) µm ODS2 column, 20 x 4.6mm (WCS hardware) µm PAC column, 20 x 4.6mm (WCS hardware) µm SAX column, 20 x 4.6mm (WCS hardware) µm SCX column, 20 x 4.6mm (WCS hardware) µm ODS3 column, 20 x 4.6mm (WCS hardware) µm C8 column, 20 x 4.6mm (WCS hardware) µm SCX column (S-P), 20 x 4.6mm (WCS hardware) HI LTD 173a

4 and Partisphere and Partisphere 2012 International Price List Part Number Description $ USD GBP EUR 10µm Semi-Prep Columns: µm SIL column, 20 x 9.4mm (Magnum-9 hardware) $ µm ODS2 column, 20 x 9.4mm (Magnum-9 hardware) $ µm ODS3 column, 20 x 9.4mm (Magnum-9 hardware) $ µm PAC column, 20 x 9.4mm (Magnum-9 hardware) $ µm SIL column, 00 x 9.4mm (Magnum-9 hardware) $ µm ODS2 column, 00 x 9.4mm (Magnum-9 hardware) $ µm ODS3 column, 00 x 9.4mm (Magnum-9 hardware) $ µm ODS column, 20 x 20mm (Magnum-20 hardware) $ µm SIL column, 00 x 20mm (Magnum-20 hardware) $ Partisphere µm Analytical Columns: (note: WVS cartridge columns require end fitting kit p/n ) Partisphere µm WAX (MAX-1) column, 20 x 4.6mm (WCS hardware) $ Partisphere µm TAC-1 (PFP) cartridge column, 26 x 4.6mm (WVS hardware) $ Partisphere µm SIL cartridge column, 12 x 4.6mm (WVS hardware) $ Partisphere µm C18 cartridge column, 12 x 4.6mm (WVS hardware) $ Partisphere µm C8 cartridge column, 12 x 4.6mm (WVS hardware) $ Partisphere µm SAX cartridge column, 12 x 4.6mm (WVS hardware) $ Partisphere µm SCX cartridge column, 12 x 4.6mm (WVS hardware) $ Partisphere µm PAC cartridge column, 12 x 4.6mm (WVS hardware) $ Partisphere µm SIL cartridge column, 20 x 4.6mm (WVS hardware) $ Partisphere µm C18 cartridge column, 20 x 4.6mm (WVS hardware) $ Partisphere µm SAX cartridge column, 20 x 4.6mm (WVS hardware) $ Partisphere µm SCX cartridge column, 20 x 4.6mm (WVS hardware) $ Partisphere µm PAC cartridge column, 20 x 4.6mm (WVS hardware) $ Analytical Cartridge Columns: (note: WVS cartridge columns require end fitting kit p/n ) µm ODS3 cartridge column, 12 x 4.6mm (WVS hardware) $ µm SAX cartridge column, 12 x 4.6mm (WVS hardware) $ µm SIL cartridge column, 20 x 4.6mm (WVS hardware) $ µm ODS3 cartridge column, 20 x 4.6mm (WVS hardware) $ µm SAX cartridge column, 20 x 4.6mm (WVS hardware) $ µm SCX cartridge column, 20 x 4.6mm (WVS hardware) $ µm ODS2 cartridge column, 20 x 4.6mm (WVS hardware) $ µm ODS3 cartridge column, 20 x 4.6mm (WVS hardware) $ µm SAX cartridge column, 20 x 4.6mm (WVS hardware) $ µm SCX cartridge column, 20 x 4.6mm (WVS hardware) $ Accessories: µm SAX column with Solvecon Kit $ µm SCX column with Solvecon Kit $ Wrenchless WVS End Fitting Kit $ WVS Guard Cartridge Holder $ WCS Guard Cartridge Holder $ Silica Guard Cartridge ( pack) $ Reverse Guard Cartridge ( pack) $ Anion Guard Cartridge ( pack) $ Cation Guard Cartridge ( pack) $ PAC Guard Cartridge ( pack) $ HI-00X PEEK Fingertight Fitting (10 pack) suitable for connection of all and Partisphere columns to all 1/16 o.d. tubing types slip free to 6000psi. $70 4 Please contact Hichrom for any or Partisphere columns not listed above 173b HI LTD

5 and Partisphere Hichrom Columns non standard Whatman/GE Healthcare dimensions Hichrom have a deserved reputation for the ability to manufacture and supply high efficiency columns packed into non standard dimensions not offered by the silica manufacturer. columns are no exception, as for many years Hichrom have manufactured columns into a wider range of column dimensions than available from Whatman/GE Healthcare. These additional dimensions will continue to be supported following the acquisition. 2.1mm i.d. Columns: 0 x x x x 2.1 (For 2.1mm i.d. Columns) (/pk) 1 SIL P-0AM P-100AM P-10AM P-20AM P-10CM C8 PC8-0AM PC8-100AM PC8-10AM PC8-20AM PC8-10CM ODS3 PODS3-0AM PODS3-100AM PODS3-10AM PODS3-20AM PODS3-10CM PAC PPAC-0AM PPAC-100AM PPAC-10AM PPAC-20AM PPAC-10CM SAX PSAX-0AM PSAX-100AM PSAX-10AM PSAX-20AM PSAX-10CM SCX PSCX-0AM PSCX-100AM PSCX-10AM PSCX-20AM PSCX-10CM (/pk) 1 10 SIL P10-0AM P10-100AM P10-10AM P10-20AM P10-10CM 10 C8 P10C8-0AM P10C8-100AM P10C8-10AM P10C8-20AM P10C8-10CM 10 ODS P10ODS-0AM P10ODS-100AM P10ODS-10AM P10ODS-20AM P10ODS-10CM 10 ODS2 P10ODS2-0AM P10ODS2-100AM P10ODS2-10AM P10ODS2-20AM P10ODS2-10CM 10 ODS3 P10ODS3-0AM P10ODS3-100AM P10ODS3-10AM P10ODS3-20AM P10ODS3-10CM 10 PAC P10PAC-0AM P10PAC-100AM P10PAC-10AM P10PAC-20AM P10PAC-10CM 10 SAX P10SAX-0AM P10SAX-100AM P10SAX-10AM P10SAX-20AM P10SAX-10CM 10 SCX P10SCX-0AM P10SCX-100AM P10SCX-10AM P10SCX-20AM P10SCX-10CM 3.2mm i.d. Columns: 0 x x x x 3.2 (for mm i.d. columns) (/pk) 1 SIL P-0AS P-100AS P-10AS P-20AS P-10C C8 PC8-0AS PC8-100AS PC8-10AS PC8-20AS PC8-10C ODS3 PODS3-0AS PODS3-100AS PODS3-10AS PODS3-20AS PODS3-10C PAC PPAC-0AS PPAC-100AS PPAC-10AS PPAC-20AS PPAC-10C SAX PSAX-0AS PSAX-100AS PSAX-10AS PSAX-20AS PSAX-10C SCX PSCX-0AS PSCX-100AS PSCX-10AS PSCX-20AS PSCX-10C (/pk) 1 10 SIL P10-0AS P10-100AS P10-10AS P10-20AS P10-10C 10 C8 P10C8-0AS P10C8-100AS P10C8-10AS P10C8-20AS P10C8-10C 10 ODS P10ODS-0AS P10ODS-100AS P10ODS-10AS P10ODS-20AS P10ODS-10C 10 ODS2 P10ODS2-0AS P10ODS2-100AS P10ODS2-10AS P10ODS2-20AS P10ODS2-10C 10 ODS3 P10ODS3-0AS P10ODS3-100AS P10ODS3-10AS P10ODS3-20AS P10ODS3-10C 10 PAC P10PAC-0AS P10PAC-100AS P10PAC-10AS P10PAC-20AS P10PAC-10C 10 SAX P10SAX-0AS P10SAX-100AS P10SAX-10AS P10SAX-20AS P10SAX-10C 10 SCX P10SCX-0AS P10SCX-100AS P10SCX-10AS P10SCX-20AS P10SCX-10C 1 Use with holder HI-161 ( 4) and column coupler HI-081 ( 23) HI LTD 173c

6 and Partisphere Hichrom Columns non standard Whatman/GE Healthcare dimensions 4.0mm i.d. Columns 0 x x x x 4.0 (for mm i.d. columns) (/pk) 1 SIL P-0AF P-100AF P-10AF P-20AF P-10C C8 PC8-0AF PC8-100AF PC8-10AF PC8-20AF PC8-10C ODS3 PODS3-0AF PODS3-100AF PODS3-10AF PODS3-20AF PODS3-10C PAC PPAC-0AF PPAC-100AF PPAC-10AF PPAC-20AF PPAC-10C SAX PSAX-0AF PSAX-100AF PSAX-10AF PSAX-20AF PSAX-10C SCX PSCX-0AF PSCX-100AF PSCX-10AF PSCX-20AF PSCX-10C (/pk) 1 10 SIL P10-0AF P10-100AF P10-10AF P10-20AF P10-10C 10 C8 P10C8-0AF P10C8-100AF P10C8-10AF P10C8-20AF P10C8-10C 10 ODS P10ODS-0AF P10ODS-100AF P10ODS-10AF P10ODS-20AF P10ODS-10C 10 ODS2 P10ODS2-0AF P10ODS2-100AF P10ODS2-10AF P10ODS2-20AF P10ODS2-10C 10 ODS3 P10ODS3-0AF P10ODS3-100AF P10ODS3-10AF P10ODS3-20AF P10ODS3-10C 10 PAC P10PAC-0AF P10PAC-100AF P10PAC-10AF P10PAC-20AF P10PAC-10C 10 SAX P10SAX-0AF P10SAX-100AF P10SAX-10AF P10SAX-20AF P10SAX-10C 10 SCX P10SCX-0AF P10SCX-100AF P10SCX-10AF P10SCX-20AF P10SCX-10C 4.6mm i.d. Columns 0 x x x x 4.6 (for mm i.d. columns) (/pk) 1 SIL P-0A P-100A P-10A P-20A P-10C C8 PC8-0A PC8-100A PC8-10A PC8-20A PC8-10C ODS3 PODS3-0A PODS3-100A PODS3-10A PODS3-20A PODS3-10C PAC PPAC-0A PPAC-100A PPAC-10A PPAC-20A PPAC-10C SAX PSAX-0A PSAX-100A PSAX-10A PSAX-20A PSAX-10C SCX PSCX-0A PSCX-100A PSCX-10A PSCX-20A PSCX-10C (/pk) 1 10 SIL P10-0A P10-100A P10-10A P10-20A P10-10C 10 C8 P10C8-0A P10C8-100A P10C8-10A P10C8-20A P10C8-10C 10 ODS P10ODS-0A P10ODS-100A P10ODS-10A P10ODS-20A P10ODS-10C 10 ODS2 P10ODS2-0A P10ODS2-100A P10ODS2-10A P10ODS2-20A P10ODS2-10C 10 ODS3 P10ODS3-0A P10ODS3-100A P10ODS3-10A P10ODS3-20A P10ODS3-10C 10 PAC P10PAC-0A P10PAC-100A P10PAC-10A P10PAC-20A P10PAC-10C 10 SAX P10SAX-0A P10SAX-100A P10SAX-10A P10SAX-20A P10SAX-10C 10 SCX P10SCX-0A P10SCX-100A P10SCX-10A P10SCX-20A P10SCX-10C 1 Use with holder HI-161 ( 4) and column coupler HI-081 ( 23) Please contact Hichrom for ordering information on any column dimension not listed 173d HI LTD