Microscopy Specs. Media refractive index
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1 Zeiss AxioImager A2 EC Plan-Neofluar ( ) EC Plan-Neofluar ( ) Plan-Apochromat ( ) Plan-Apochromat ( ) Plan-Apochromat Korr ( ) Plan-Apochromat DIC Oil ( ) Microscopy Specs Mag NA Immersion n Working Condenser Turret Numerical aperture Media refractive index Distance Bright Field DIC 2.5x AIR mm H no 5x 0.16 AIR mm H no 10x 0.45 AIR mm H II 20x 0.80 AIR mm H II 40x 0.95 AIR mm H III 63x 1.40 OIL mm H III Camera: AxioCam 512 Color Software: Zen Blue Pro with Multichannel Fluorescent Light Source: Lumencor SOLA-365-SE light engine Filters: Position 1: DAPI narrow - Chroma Custom Set Excitation: 365/10x; dichroic mirror: T 387 LP; Emission filter: 400 LP Position 2: FITC AF488/FITC/EGFP plus chlorophyll auto-fluorescence - Chroma Filter set Excitation: BP 480/40; Beamsplitter: FT 510; Emission: 510 LP MICROSCOPE SPECS Axioimager
2 Position 3: GFP - AF488/FITC/EGFP minus chlorophyll auto-fluorescence - Zeiss Filter set 38 ( ) Excitation: BP 470/40; Beamsplitter: FT 495; Emission: BP 525/50 Position 4: Rhodamine Zeiss Filter set 20 ( ) Excitation: BP 546/12; beamsplitter: FT 560; emission: BP Position 5: DIC Analyzer for DIC imaging with transmitted light Position 6: Empty for bright-field and dark-field imaging with transmitted light Previous specs: Before March 22, 2018: Camera: AxioCam HRc Software: Zen Blue light Fluorescent Light Source and filters: NONE Before May 4, 2017: Software: AxioVision 4 MICROSCOPE SPECS Axioimager
3 Zeiss Axiophot Plan-Neofluar (440320) Plan-Neofluar Pol (440333) Plan-Neofluar Pol (440343) Plan-Neofluar Pol (440353) Plan-Apochromat DIC Oil (440762) Mag NA Numerical aperture Immersion Media n refractiv e index Working Distance Condenser Turret Bright DIC Field 5x 0.15 AIR mm H no 10x 0.30 AIR mm H DIC x 0.50 AIR mm H DIC x 0.85 AIR mm H DIC x 1.40 OIL mm H DIC Camera: AxioCam 512 Color Software: Zen Blue with multichannel module Fluorescent Light Source: Lumencor SOLA-365-SE light engine Fluorescent Filters: Position I: DAPI narrow - Zeiss Filter set 1 (48702) Excitation: 365/12; dichroic mirror: FT 395; Emission filter: LP397 Position II: DAPI wide - Chroma Filter Set Excitation: 350/50; dichroic mirror: 400; Emission filter: 460/50 MICROSCOPE SPECS Axiophot
4 Position III: FITC - AF488/FITC/EGFP plus chlorophyll auto-fluorescence - Zeiss Filter Set 9 (488009) Excitation: ; dichroic mirror: 510; Emission filter: LP515 Position IV: Rhodamine - Zeiss Filter Set 15 (488015) Excitation: 546/12; FT580; Emission filter: LP590 Previous specs: Before May 4, 2017: Camera: AxioCam HRc Software: AxioVision 4 Before March 21, 2015: Light Source: HBO-100W Fluorescent Filter Position II: DAPI wide - Zeiss Filter Set 2 (487902) Excitation: 365; dichroic mirror: FT 395; Emission filter: LP420 MICROSCOPE SPECS Axiophot
5 Zeiss Axioskop Plan-Neofluar (440310) Plan-Neofluar Ph1 (440330) Plan-Neofluar Ph2 (440341) Plan-Neofluar Ph2 (440351) Mag NA Numerical aperture Immersion Media n refractive index Working Distance Condenser Turret Bright DIC Field 2.5x AIR mm H/DIC no 10x 0.30 AIR mm H/DIC DIC 20x 0.50 AIR mm H/DIC DIC 40x 0.75 AIR mm H/DIC DIC Camera: AxioCam HRc Software: Zen Blue with multichannel module Fluorescent Light Source: Lumencor SOLA-365-SM light engine Fluorescent Filters: Position I: DAPI narrow - Zeiss Filter set 1 (48702) Excitation: 365/12; dichroic mirror: FT 395; Emission filter: LP397 Position II: Rhodamine - Zeiss Filter Set 15 (488015) Excitation: 546/12; FT580; Emission filter: LP590 MICROSCOPE SPECS Axioskop
6 Position III: AF488 - AF488/FITC/EGFP plus chlorophyll auto-fluorescence - Zeiss Filter Set 9 (488009) Excitation: ; dichroic mirror: 510; Emission filter: LP515 Previous specs: Before March 22, 2018: Camera: Axiocam MRc Before May 4, 2017: Camera: None Software: None Before March 21, 2015: Light source: HBO-50W Fluorescent MICROSCOPE SPECS Axioskop
7 Zeiss LSM 700 confocal Plan-Apochromat ( ) Plan-Apochromat ( ) Plan-Apochromat Korr ( ) Plan-Apochromat Oil ( ) Plan-Apochromat DIC Oil ( ) Alpha Plan-Apochromat DIC Oil ( ) W N-Achroplan ( ) Mag NA Numerical aperture Immersion Media n refractive index Working Distance Condenser Turret Bright DIC Field 10x 0.45 AIR mm BF DIC II 20x 0.80 AIR mm BF DIC II 40x 0.95 AIR mm BF DIC III 40x 1.3 OIL mm BF DIC III 63x 1.40 OIL mm BF DIC III 100x 1.46 OIL mm BF DIC III 20x 0.5 WATER mm BF The 20x/0.5 WD 2.6 M27 W N-Achroplan objective is for dipping directly into the water/medium on the slide or petri dish. It is not generally installed on the microscope. Please see Faye Rosin if you would like to use this objective. Microscope: Upright AxioImager.Z2 Confocal Imaging: Confocal Software: Zen Black (2.3) Laser Lines: nm (e.g. DAPI) nm (e.g. GFP, FITC, CY2) nm (e.g. DsRed, CY3, mcherry) nm (e.g. CY5) Wide field imaging (Brightfield/DIC/fluorescence): Software: Zen Blue (2.3) with Z-stack, time-lapse, multichannel, tiles modules Camera: AxioCam 512 Color Fluorescent Light Source: Lumencor SOLA-365-SE light engine Filters: Position 1: DAPI narrow - Chroma Custom Set Excitation: 365/10x; dichroic mirror: T 387 LP; Emission filter: 400 LP MICROSCOPE SPECS Confocal
8 Postion 2: DAPI wide - Zeiss Filter set 49 ( ) Excitation: G 365; Beamsplitter: FT 395; Emission: BP 445/50 Position 3: GFP - AF488/FITC/EGFP minus chlorophyll auto-fluorescence - Chroma filter set Excitation: BP 470/40; Beamsplitter: T 495; Emission: BP 525/50 Position 4: Rhodamine - Zeiss Filter set 20 ( ) Excitation: BP 546/12; beamsplitter: FT 560; emission: BP Position 5: DIC Analyzer for DIC imaging with transmitted light Position 6: Empty for bright-field and dark-field imaging with transmitted light Previous specs: Before March 1, 2018 Confocal Software: Zen Black 2010 Wide field imaging Software: AxioVision 4 software, mosaix and Z-Stack modules Before May 4, 2017: Camera: AxioCam HRc MICROSCOPE SPECS Confocal
9 Before March 21, 2015: Fluorescent light source: HBO 100-W Before April 1, 2015: Filters: GFP - Zeiss Filter set 38 ( ) Excitation: BP 470/40; Beamsplitter: FT 495; Emission: BP 525/50 MICROSCOPE SPECS Confocal
10 Zeiss Microspectrophotometer Plan-Neofluar (440330) Plan-Neofluar (440340) Plan-Neofluar Pol (44 ) Plan-Neofluar Oil (440480) Plan-Neofluar Oil Iris (440486) Mag NA Numerical aperture Immersion Media n refractive index Working Distance mm Condenser Turret Bright DIC Field 10x 0.30 AIR H DIC x 0.50 AIR H DIC x 0.75 AIR H DIC x 1.30 OIL H DIC x 1.30 OIL H Microspectrophotometer: MSP 20 Fluorescent Light Source: HBO 100-W burner Fluorescent Filters: Position I: DAPI narrow - Zeiss Filter set (48702) Excitation: G 365/12 nm dichroic mirror: FT 395; emission: LP397 MICROSCOPE SPECS Microspectrophotometer
11 Zeiss Discovery v12 Dissecting microscope PlanApo S ( ) PlanApo S ( ) PlanApo S ( ) Immersion Working Mag Media Distance 0.63x AIR 81 mm 1.5x AIR 30 mm 2.3x AIR 10 mm Camera: AxioCam 512 Color Software: Zen Blue Pro with extended focus and time lapse modules Previous specs: Before May 4, 2017: Camera: AxioCam HRc Software: AxioVision 4 with extended focus and time lapse modules Zeiss Stemi SV 11 Plan Apochromat S ( ) Plan S ( ) Camera: AxioCam HRc Software: Zen Blue Mag Immersion Media 1.0x AIR 1.6x AIR Previous specs: Before May 4, 2017: Software: AxioVision 4 NightSea Fluorescence Adapters for dissecting scopes Filter Set Excitation Emission Fluorophores Green Only (RB-GO) nm nm BP EGFP, fluorescein Ultraviolet (UV) nm 415nm LP DAPI, Aniline Blue MICROSCOPE SPECS Dissecting Scopes
12 JEOL JSM-6010 LV Scanning Electron Microscope Resolution: 4.0 nm in HV mode; 5.0 nm in LV mode Magnification: 80X to 300kX Accelerating Voltage: 500 V to 20 kv Detectors: 1. Everhart Thornley 2. Backscatter with COMPO, TOPO, and Shadow 3. Low Vacuum Secondary Electron detector Operating Pressure: 10-4 Pa order to 100Pa Other: motorized 2 axis stage, chamberscope, stage navigation system MICROSCOPE SPECS SEM
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